CN106829903A - The method that one step prepares flower-shaped calcium phosphate microsphere - Google Patents
The method that one step prepares flower-shaped calcium phosphate microsphere Download PDFInfo
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- CN106829903A CN106829903A CN201710069528.8A CN201710069528A CN106829903A CN 106829903 A CN106829903 A CN 106829903A CN 201710069528 A CN201710069528 A CN 201710069528A CN 106829903 A CN106829903 A CN 106829903A
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- calcium phosphate
- gelatin
- phosphate microsphere
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B25/00—Phosphorus; Compounds thereof
- C01B25/16—Oxyacids of phosphorus; Salts thereof
- C01B25/26—Phosphates
- C01B25/32—Phosphates of magnesium, calcium, strontium, or barium
- C01B25/325—Preparation by double decomposition
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B25/00—Phosphorus; Compounds thereof
- C01B25/16—Oxyacids of phosphorus; Salts thereof
- C01B25/26—Phosphates
- C01B25/38—Condensed phosphates
- C01B25/42—Pyrophosphates
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- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2002/00—Crystal-structural characteristics
- C01P2002/70—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data
- C01P2002/72—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data by d-values or two theta-values, e.g. as X-ray diagram
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/01—Particle morphology depicted by an image
- C01P2004/03—Particle morphology depicted by an image obtained by SEM
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/01—Particle morphology depicted by an image
- C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/30—Particle morphology extending in three dimensions
- C01P2004/32—Spheres
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/60—Particles characterised by their size
- C01P2004/61—Micrometer sized, i.e. from 1-100 micrometer
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Abstract
The invention discloses a kind of method that a step prepares flower-shaped calcium phosphate microsphere, comprise the following steps：(1) gelatin is added to the water dissolving, is stirred in 26 DEG C of oil bath pan, and sodium pyrophosphate is added in whipping process, be further continued for stirring, obtain gelatin sodium pyrophosphate nano-complex；(2) 30 DEG C are warming up to calcium chloride is added in the gelatin sodium pyrophosphate nano-complex in step (1), 15min is stirred, sediment are collected in centrifugation, then with pure water is resuspended, washing precipitate 2~3 times；(3) it is step (2) products therefrom is lyophilized, obtain style calcium phosphate microsphere.Just reaction can be quickly completed under normal temperature and pressure of the present invention, and without the product of pollution is produced to environment, raw material is cheap and easy to get, low production cost.
Description
Technical field
The present invention relates to biological medicine technical field of material, and in particular to an a kind of step prepares flower-shaped calcium phosphate microsphere
Method.
Background technology
Wound, tumour, inflammation etc. can result in different degrees of bone tissue and lose or defect, and bone tissue defect is very
Society, motion and the mental functioning of patient are influenceed in big degree；Even if only a small amount of bone tissue lose also can in function and
Patient is impacted in two aspects attractive in appearance.
And the treatment of bone tissue defect is the common disease and difficult treatment of the departments such as orthopaedics, the department of stomatology, in clinic, we use
Various bone alternate materials carry out the treatment of Cranial defect, and conventional method has autologous bone transplanting and bone xenograft.
Autologous bone transplanting has preferable curative effect and reliability in the treatment of various bone tissue defects, is considered as a long time bone
" goldstandard " of implantation technique, however, the risk of various complication limits the use of this technology；Bone xenograft technology is returned
The a variety of unfavorable of autologous bone transplanting technology is kept away, but has been the increase in viral communication equivalent risk；Therefore, various artificial materials are used for
The reparation of bone tissue defect, calcium phosphate is the Typical Representative with excellent osteogenic action material, and various calcium phosphate materials are proved
With good biocompatibility and osteoinductive, and commodity are converted into, are widely used in the fields such as the department of stomatology, orthopaedics；With
The development that becomes more meticulous of material preparation process, people start to probe into nanoscale structures under different calcium phosphate patterns for osteogenic action
Influence, this also proposes requirement to building the calcium phosphate material with specific morphology.
The content of the invention
For above-mentioned deficiency of the prior art, the present invention provides a kind of method that a step prepares flower-shaped calcium phosphate microsphere,
Form is undesirable during it efficiently solves style calcium phosphate material preparation at this stage, and reaction condition requirement is high, and the reaction time is long, produces
The low problem of rate.
The technical solution adopted by the present invention is：
A kind of method that a step prepares flower-shaped calcium phosphate microsphere, comprises the following steps：
(1) gelatin is added to the water dissolving, regulation solution concentration is 1~2mg/mL, is stirred in 26 DEG C of oil bath pan, and
The sodium pyrophosphate that concentration is 90~120mg/mL is added in whipping process, is further continued for stirring 10min, obtain gelatin-sodium pyrophosphate
Nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 15~20:1~3；
(2) to the calcium chloride that addition concentration in the gelatin in step (1)-sodium pyrophosphate nano-complex is 90~100mM,
It is warming up to 30 DEG C, stirs 15min, then in 764G, 5min is centrifuged, collects sediment, then with pure water is resuspended, washing precipitate 2
~3 times；Wherein, calcium chloride and the mass ratio of gelatin are 5~8:1~3；
(3) it is step (2) products therefrom is lyophilized, obtain style calcium phosphate microsphere.
Further, gelatin solution concentration is 2mg/mL in step (1).
Further, sodium pyrophosphate solution concentration is 100~120mg/mL in step (1).
Further, pyrophosphate and the mass ratio of gelatin are 20 in step (1):1.
Further, calcium chloride concentration is 100mM in step (2).
Further, calcium chloride and the mass ratio of gelatin are 7.78 in step (2):1.
Further, style calcium phosphate microsphere particle diameter is 2~6 μm in step (3).
The style calcium phosphate microsphere that above-mentioned preparation method is prepared.
Beneficial effects of the present invention are：
Nano-complex is cross-linked to form by gelatin and calcium phosphate ion, and adds calcium salt on this basis, using subdivision
Solution reaction forms calcium phosphate crystal on nano-complex, so as to guide and form the calcium phosphate microsphere with style pattern.
The calcium phosphate microsphere particle diameter that this method is obtained is between 2~6 microns, and good dispersion is reproducible, has regular
Petal-like stratiform secondary structure, style structure is the porous layering of a class, the structure type of 3 D stereo, with high ratio table
Area.
The present invention it is raw materials used it is cheap, be easy to get, course of reaction is gentle, is reacted using the ionomer of gelatin, pyrophosphate
And the metathesis reaction of phosphate and calcium salt, reaction just can be quickly completed under normal temperature and pressure, reduce production cost, it is easy to work
Industryization is promoted.
It is not related to any link and product for being produced to environment and being polluted in process of the present invention, is a kind of green and cheap conjunction
Into method.
Brief description of the drawings
Fig. 1 is the SEM figures of calcium phosphate microsphere prepared by the present invention；
Fig. 2 is the HRTEM figures of calcium phosphate microsphere prepared by the present invention；
Fig. 3 is the XRD of calcium phosphate microsphere prepared by the present invention；
Fig. 4 is the BET figures of calcium phosphate microsphere prepared by the present invention；
Fig. 5 is CCK8 kit toxicity test result figures；
Fig. 6 is ALP genes the 3rd day and the 14th day PCR data figure；
Fig. 7 is the 3rd day PCR data figure of ON genes；
Fig. 8 is the 14th day PCR data figure of ON genes；
Fig. 9 is the 3rd day PCR data figure of RUNX2 genes；
Figure 10 is the 14th day PCR data figure of RUNX2 genes；
Figure 11 is OCN genes the 3rd day and the 14th day PCR data figure；
Figure 12 is BMP4 genes the 3rd day and the 14th day PCR data figure；
Figure 13 is BMP7 genes the 3rd day and the 14th day PCR data figure.
Specific embodiment
Specific embodiment of the invention is described below, this hair is understood in order to those skilled in the art
It is bright, it should be apparent that the invention is not restricted to the scope of specific embodiment, for those skilled in the art,
As long as in appended claim restriction and the spirit and scope of the present invention for determining, these changes are aobvious and easy to various change
See, all are using the innovation and creation of present inventive concept in the row of protection.
Embodiment 1
A kind of method that a step prepares style calcium phosphate microsphere powder, comprises the following steps：
(1) take 3mL pigs source gelatin and be added to the water dissolving, regulation solution concentration is 1mg/mL, in 26 DEG C of oil bath pan
Temperature constant magnetic stirring, and 1.2mL is added in whipping process, concentration is the sodium pyrophosphate of 100mg/mL, is further continued for stirring
10min, obtains gelatin-sodium pyrophosphate nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 15:1；
(2) 4.2mL is added to the gelatin in step (1)-sodium pyrophosphate nano-complex, concentration is the calcium chloride of 90mM,
It is warming up to 30 DEG C, magnetic agitation 15min, then in 764G, is centrifuged 5min, collects sediment, then with pure water is resuspended, washing is precipitated
Thing 2 times；Wherein, calcium chloride and the mass ratio of gelatin are 5:1；
(3) step (2) products therefrom is freezed in vacuum freeze drier, obtains style calcium phosphate microsphere.
Embodiment 2
(1) take 3mL pigs source gelatin and be added to the water dissolving, regulation solution concentration is 2mg/mL, in 26 DEG C of oil bath pan
Temperature constant magnetic stirring 10min, and 1.2mL is added in whipping process, concentration is the sodium pyrophosphate of 100mg/mL, obtains gelatin-Jiao
Sodium phosphate nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 20:1；
(2) 4.2mL is added to the gelatin in rapid (1)-sodium pyrophosphate nano-complex, concentration is the calcium chloride of 100mM, is risen
To 30 DEG C, magnetic agitation 15min, then in 764G, is centrifuged 5min to temperature, collects sediment, then with pure water is resuspended, washing precipitate
3 times；Wherein, calcium chloride and the mass ratio of gelatin are 8:1；
(3) step (2) products therefrom is freezed in vacuum refrigerating machine, obtains style calcium phosphate microsphere.
Embodiment 3
(1) take 3mL calves source gelatin and be added to the water dissolving, regulation solution concentration is 2mg/mL, in 26 DEG C of oil bath pan
Middle temperature constant magnetic stirring 10min, and 1.2mL is added in whipping process, concentration is the sodium pyrophosphate of 100mg/mL, obtain gelatin-
Sodium pyrophosphate nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 20:1；
(2) 4.2mL is added to the gelatin in step (1)-sodium pyrophosphate nano-complex, concentration is the calcium chloride of 100mM,
It is warming up to 30 DEG C, magnetic agitation 15min, then in 764G, is centrifuged 5min, collects sediment, then with pure water is resuspended, washing is precipitated
Thing 3 times；Wherein, calcium chloride and the mass ratio of gelatin are 7.78:1；
(3) step (2) products therefrom is freezed in vacuum refrigerating machine, obtains style calcium phosphate microsphere.
Embodiment 4
(1) take 3mL pigs source gelatin and be added to the water dissolving, regulation solution concentration is 2mg/mL, in 26 DEG C of oil bath pan
Temperature constant magnetic stirring 10min, and 1.2mL is added in whipping process, concentration is the sodium pyrophosphate of 100mg/mL, obtains gelatin-Jiao
Sodium phosphate nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 20:1；
(2) 4.2mL is added to the gelatin in step (1)-sodium pyrophosphate nano-complex, concentration is the calcium chloride of 100mM,
It is warming up to 30 DEG C, magnetic agitation 15min, then in 764G, is centrifuged 5min, collects sediment, then with pure water is resuspended, washing is precipitated
Thing 3 times；Wherein, calcium chloride and the mass ratio of gelatin are 7.78:1；
(3) step (2) products therefrom is freezed in vacuum refrigerating machine, obtains style calcium phosphate microsphere.
Embodiment 5
(1) take 3mL pigs source gelatin and be added to the water dissolving, regulation solution concentration is 2mg/mL, in 26 DEG C of oil bath pan
Temperature constant magnetic stirring 10min, and 1.2mL is added in whipping process, concentration is the sodium pyrophosphate of 120mg/mL, obtains gelatin-Jiao
Sodium phosphate nano-complex；Wherein, pyrophosphate and the mass ratio of gelatin are 20:1；
(2) 4.2mL is added to the gelatin in step (1)-sodium pyrophosphate nano-complex, concentration is the calcium chloride of 100mM,
It is warming up to 30 DEG C, magnetic agitation 15min, then in 764G, is centrifuged 5min, collects sediment, then with pure water is resuspended, washing is precipitated
Thing 3 times；Wherein, calcium chloride and the mass ratio of gelatin are 8:1；
(3) step (2) products therefrom is freezed in vacuum refrigerating machine, obtains style calcium phosphate microsphere.
Experimental example
1st, the calcium phosphate microsphere prepared using the electron microscope scanning embodiment of the present invention 3, its result is shown in Fig. 1.
2nd, the calcium phosphate microsphere prepared using the transmission electron microscopy embodiment of the present invention 3, its result is shown in Fig. 2.
3rd, X-ray diffraction is carried out to the calcium phosphate microsphere that the embodiment of the present invention 3 is prepared, its result is shown in Fig. 3.
4th, the calcium phosphate microsphere that the embodiment of the present invention 3 is prepared is detected using Fourier infrared spectrograph, its
Detection collection of illustrative plates is shown in Fig. 4.
5th, the cell toxicant of calcium phosphate microsphere that the embodiment of the present invention 3 prepares to BMSC is probed into using CCK8 kits
Property, it the results are shown in Table 1 and Fig. 5.
The calcium phosphate microsphere material tests data of table 1
| μg/mL | O.D. | RGR (%) |
| 0 | 1.061~1.100 | 100.00±1.84 |
| 1 | 1.006~1.139 | 97.72±6.55 |
| 5 | 1.019~1.123 | 99.75±4.33 |
| 10 | 1.060~1.107 | 100.28±1.80 |
| 15 | 1.023~1.068 | 96.67±1.80 |
| 20 | 0.977~1.102 | 94.41±6.46 |
| 50 | 0.876~1.095 | 95.13±0.37 |
| 100 | 0.621~0.936 | 74.64±11.11 |
| 200 | 0.275~0.434 | 35.64±6.05 |
| 400 | 0.203~0.342 | 27.92±5.35 |
| 600 | 0.289~0.329 | 28.42±1.51 |
6th, the calcium phosphate microsphere of the 10 μ g/mL concentration that the embodiment of the present invention 3 is prepared is probed into alkaline phosphorus using qPCR
The shadow of sour enzyme (ALP), Integrated implant element (ON and RUNX2), BGP (OCN) and bone morphogenetic protein (BMP4 and BMP7)
Ring, its result is shown in Fig. 6~13.
Interpretation of result
As shown in figure 1, material by multiple lamellas stacking form, overall pattern be in porous style, measure its a diameter of 3~5
Micron.
As shown in Fig. 2 calcium phosphate microsphere material is formed by stacking by laminar structured, there is a hollow knot inside microballoon
Structure.
Fig. 3 and Fig. 4 show the mixture of phosphoric acid the calcium component predominantly calcium pyrophosphate and calcium triphosphate of material, and both
Calcium phosphate is good biocompatibility, and thus the preferable material of bone formation performance, improves the life of the product that the present invention is prepared
Thing compatibility.
Can be drawn by Fig. 5 and Biao 1, when material concentration is less than 50 μ g/mL, it has preferable bio-compatible for BMSC
Property.
Can be drawn by Fig. 6~13, alkaline phosphatase (ALP), Integrated implant element (ON and RUNX2), BGP (OCN) and bone
Morphogenetic proteins (BMP4 and BMP7) gene had different degrees of rise at the 3rd day of the observation period and the 14th day.
Claims (8)
1. a kind of method that a step prepares flower-shaped calcium phosphate microsphere, it is characterised in that comprise the following steps：
(1) gelatin is added to the water dissolving, regulation solution concentration is 1~2mg/mL, is stirred in 26 DEG C of oil bath pan, and stirring
The sodium pyrophosphate that concentration is 90~120mg/mL is added during mixing, is further continued for stirring 10min, obtain gelatin-sodium pyrophosphate nanometer
Compound；Wherein, pyrophosphate and the mass ratio of gelatin are 15~20:1~3；
(2) to the calcium chloride that concentration is 90~100mM is added in the gelatin in step (1)-sodium pyrophosphate nano-complex, heat up
To 30 DEG C, 15min is stirred, then in 764G, 5min is centrifuged, collect sediment, then with pure water is resuspended, washing precipitate 2~3
It is secondary；Wherein, calcium chloride and the mass ratio of gelatin are 5~8:1~3；
(3) it is step (2) products therefrom is lyophilized, obtain style calcium phosphate microsphere.
2. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (1)
Gelatine solution concentration is 2mg/mL.
3. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (1)
Sodium pyrophosphate solution concentration is stated for 100~120mg/mL.
4. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (1)
It is 20 that pyrophosphate is stated with the mass ratio of gelatin:1.
5. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (2)
Calcium chloride concentration is stated for 100mM.
6. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (2)
It is 7.78 that calcium chloride is stated with the mass ratio of gelatin:1.
7. the method that a step according to claim 1 prepares flower-shaped calcium phosphate microsphere, it is characterised in that institute in step (3)
Style calcium phosphate microsphere particle diameter is stated for 2~6 μm.
8. style calcium phosphate microsphere obtained in the preparation method described in any one of claim 1~7.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759169A (en) * | 2009-11-13 | 2010-06-30 | 中国科学院上海硅酸盐研究所 | Calcium phosphate nano-structure hollow microsphere and preparation method thereof |
| CN101850133A (en) * | 2010-06-01 | 2010-10-06 | 华南理工大学 | Self-setting calcium phosphate micro spheres, method for preparing same and application thereof |
| CN105858629A (en) * | 2016-03-30 | 2016-08-17 | 武汉亚洲生物材料有限公司 | Preparation method for inorganic microspheres, inorganic microspheres prepared thereby, and application of inorganic microspheres |
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- 2017-03-01 CN CN201710069528.8A patent/CN106829903A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759169A (en) * | 2009-11-13 | 2010-06-30 | 中国科学院上海硅酸盐研究所 | Calcium phosphate nano-structure hollow microsphere and preparation method thereof |
| CN101850133A (en) * | 2010-06-01 | 2010-10-06 | 华南理工大学 | Self-setting calcium phosphate micro spheres, method for preparing same and application thereof |
| CN105858629A (en) * | 2016-03-30 | 2016-08-17 | 武汉亚洲生物材料有限公司 | Preparation method for inorganic microspheres, inorganic microspheres prepared thereby, and application of inorganic microspheres |
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Application publication date: 20170613 |