CN106824315A - The device and method of micro- liquid heating in a kind of detection zone of array chip - Google Patents
The device and method of micro- liquid heating in a kind of detection zone of array chip Download PDFInfo
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- CN106824315A CN106824315A CN201710101719.8A CN201710101719A CN106824315A CN 106824315 A CN106824315 A CN 106824315A CN 201710101719 A CN201710101719 A CN 201710101719A CN 106824315 A CN106824315 A CN 106824315A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
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Abstract
The invention discloses a kind of device and method of micro- liquid heating in detection zone of array chip,The device includes piezoelectric substrate,Each making has row's interdigital transducer in four side regions of the working surface of piezoelectric substrate,Middle section restocking is provided with an array chip and is placed with oil phase microfluid,Array chip includes PDMS condensates,The vertical through hole of multiple arranged with array way is offered on PDMS condensates,Metal heat transfer piece is sealed with its lower port and forms detection zone,Metal heat transfer piece is connected with metal heat transfer post,PDMS cushion blocks are respectively housed on the corner of the polymeric bottoms of PDMS,The bottom of metal heat transfer post is not in contact with each other with the working surface of piezoelectric substrate,The surface acoustic wave that interdigital transducer is excited is acted on oil phase microfluid,The heat of generation heats the micro- reaction solution in detection zone after metal heat transfer post and metal heat transfer piece；Advantage is micro- liquid that surface acoustic wave is heated in detection zone indirectly, it is to avoid active influence of the micro- liquid of surface acoustic wave direct radiation to biomolecule in micro- liquid.
Description
Technical field
The present invention relates to a kind of micro- liquid heating technique, the detection of array chip is realized more particularly, to a kind of surface acoustic wave
The device and method of micro- liquid heating in area.
Background technology
Biochip is an emerging technology, batch that it can on a single chip realize biomass and chemical quantity, fast
Speed, Accurate Analysis, its advantage for protruding is that device volume is small, reagent consumption is few, can avoid the mistake that is artificially introduced in analysis
Difference etc., it is paid attention to by numerous experts, scholar, and has turned into an important research focus of analysis field, in bioengineering, ring
The fields such as border monitoring, illicit drugs inspection and food security are applied widely.Array chip is a kind of important of biochip
Structure type, its detection zone that array structure is built on substrate, to realize batch detection.In the exploration of biochemical reaction condition
In research, different biochemical reaction conditions are often adjusted respectively in the detection zone of array, to study extraneous biochemical reaction condition
Influence to detecting performance.Temperature is an important factor in order of biochemical reaction, therefore, to detection zone in micro- reaction solution
Temperature control is an emphasis research topic of biochip.For this reason, it may be necessary to study adding for the particular detection area of array chip
Thermal means and its temperature characterisitic.
The heating means of the micro- reaction solution in the detection zone of biochip have various.The experts such as Xi Wenzhu use microelectronics work
Skill makes PCR biochips, and NiCr metallic film microheaters are made on the reaction microchamber of PCR biochips, with full
The requirement of sufficient PCR biochip temperatures characteristic.Dai Jing is proposed using transparent indium tin oxide film glass as heating element heater, is realized
The heating of the micro- reaction solution on transparent indium tin oxide film glass, makes the temperature of micro- reaction solution rise 50 DEG C.Also expert adopts
With RTD and Ti electrode as micro- heating unit, and it is integrated on miniflow substrate, realizes the heating of micro- reaction solution, and successfully
It is applied to RT-polymerase chain reaction quantitative determination tumour virus.On this basis, scholar proposes with platinum as material, answer
Array type micro-heater is made on a glass substrate of microelectronic technique so that heat endurance and uniformity are obtained in detection zone
Very big improvement.These integrated micro-heaters on microflow analysis chip can well realize that micro- reaction solution is to reaction temperature on piece
The requirement of degree, thus, applied in different occasions, but they cannot be applied to the piezoelectricity microflow analysis with critical role
On chip.
Piezoelectric device is because with process is simple, maturation, low cost and other advantages are widely used to the communications field.In recent years
Come, surface acoustic wave has powerful miniflow manipulation ability gradually by micro-fluidic expert fully realizes and gradually obtains weight
Depending on, and realized on piezoelectric substrate microfluid transport, mix, extracting, a series of microfluidic manipulations such as biomolecule fast enriching
And miniflow biochemical analysis.But the heating means of micro- reaction solution are all difficult in piezoelectric micromotor flow point on the above-mentioned microflow analysis chip for referring to
Realized on analysis chip, cannot also be realized using the above method micro- anti-in any detection zone of surface acoustic wave heating electrodes formula chip
Liquid is answered, it is therefore desirable to solve the consistency problem of the heating unit and piezoelectric substrate in current microflow analysis chip, to carry simultaneously
Heat affected zone high is heated accuracy and flexibility.
Although having document report using the micro- reaction solution on surface acoustic wave heating piezoelectric substrate.Such as periodical《Ultrasound, ferroelectricity and
FREQUENCY CONTROL IEEE proceedings》The 10th 1881-1883 pages of phase (IEEE, Transactions on of volume 52 in 2005
ultrasonics,Ferroelectrics and Frequency control,Vol.52(10),2005:It is 1881-1883) public
Open《The liquid fuel factor that surface acoustic wave acoustic streaming causes on piezoelectric substrate》(《Liquid heating effects by SAW
streaming on the piezoelectric substrate》), it makes one on piezoelectric substrate using microelectronic technique
To interdigital transducer, electric signal is added on interdigital transducer after amplifying through power amplifier, surface acoustic wave is excited, on acoustic propagation road
Fitted on footpath a filter paper bar, the micro- liquid of sample introduction on filter paper bar, surface acoustic wave uploads sowing time in piezoelectric substrate, runs into micro- on filter paper bar
Liquid, energy radiation liquid in a subtle way, heats micro- liquid, improves micro- liquid temperature degree, and the micro- liquid temperature degree on filter paper bar increases with added electric signal power
Increase greatly, and reach dynamic equilibrium within a certain period of time, i.e., after the temperature of micro- liquid rises to uniform temperature, surface acoustic wave spoke
Inject the energy that the energy of micro- liquid outwardly discharges with micro- liquid and reach balance, the temperature of micro- liquid does not continue to rise.The method can
Effectively to realize that the micro- liquid on piezoelectric substrate is heated, can make full use of piezoelectric substrate that the advantage of ability, but office are manipulated to micro- liquid
It is limited to micro- liquid to be close on piezoelectric substrate, and surface acoustic wave continues direct radiation liquid in a subtle way, biomolecule is (such as in reducing micro- liquid
Enzyme, protein etc.) activity；And micro- liquid cannot be flexibly heated using single drop transfer ways, limited flexibility needs to be changed
Enter.
The content of the invention
The technical problems to be solved by the invention be to provide a kind of surface acoustic wave realize it is micro- in the detection zone of array chip
The device and method of liquid heating, the apparatus structure is simple, easy to use, small volume, be easily integrated, and surface acoustic wave indirectly, essence
The micro- liquid in detection zone is heated accurately, it is to avoid active shadow of the micro- liquid of surface acoustic wave direct radiation to biomolecule in micro- liquid
Ring.
The present invention solve the technical scheme that is used of above-mentioned technical problem for：Micro- liquid in a kind of detection zone of array chip
The device of heating, it is characterised in that including piezoelectric substrate, the upper surface of described piezoelectric substrate is working surface, described piezoelectricity
It is each in four side regions of the working surface of substrate to make the interdigital transducer for having a row for exciting surface acoustic wave, described piezoelectricity
The middle section restocking of the working surface of substrate is provided with an array chip, and described array chip is polymerized including PDMS
Body, offers the multiple arranged with array way through holes, the lower end of described vertical through hole vertically on described PDMS condensates
The detection zone for placing micro- reaction solution that metal heat transfer piece forms upper end open and lower end closed, described gold are sealed with mouth
The bottom for belonging to heat transfer sheet is connected with metal heat transfer post, and a PDMS is respectively provided with the corner of the polymeric bottoms of described PDMS
Cushion block, described PDMS condensates are by the padded worksheet to be set up in described piezoelectric substrate of the PDMS cushion blocks described in four
On the middle section in face, and the bottom of described metal heat transfer post is set to be not in contact with each other with the working surface of described piezoelectric substrate,
Oil phase microfluid, the sound that described interdigital transducer is excited are placed with the middle section of the working surface of described piezoelectric substrate
Surface wave is acted on described oil phase microfluid, the heat that described oil phase microfluid is produced through described metal heat transfer post and
Micro- reaction solution in the described detection zone of described metal heat transfer piece heat conduction heating.
It is adjacent in the interdigital transducer described in a row made in every side region of the working surface of described piezoelectric substrate
The spacing distance between interdigital transducer described in two is 0.4~0.6 millimeter.Here, limiting two neighboring interdigital transducer
Between spacing distance, be that the surface acoustic wave excited in order to ensure interdigital transducer can act on worksheet positioned at piezoelectric substrate
On oil phase microfluid on the middle section in face, oil phase microfluid is set to move to any one detection zone of array chip
Underface such that it is able to the heating to the micro- reaction solution in the detection zone is realized using surface acoustic wave and oil phase microfluid；If
Spacing distance between two neighboring interdigital transducer is too big, then do not reach above-mentioned purpose very likely；If two neighboring fork
Spacing distance between finger transducer is too small, then the quantity of interdigital transducer can increase, and technology difficulty can also increase, so as to will lead
Cause cost increase.
The polymeric thickness of described PDMS is that the height of described vertical through hole is 2~3 millimeters.Due to vertical through hole
Height is relevant with the amount of micro- reaction solution, thus PDMS condensates too it is thick need not, and it is too thin if to reach one in detection zone
Fixed capacity, then require being relatively large in diameter for vertical through hole, and must so increase in the case of same detection number in array chip
Plus the area of array chip, the area of piezoelectric substrate is also increased, substantially increase the device cost of the device.
The size of described metal heat transfer piece more than described vertical through hole aperture, it is described vertical to cover all
The lower port of through hole.Can be by the milli of aperture 1~3 being designed and sized to more than vertical through hole of metal heat transfer piece in actual design
Rice, to ensure that metal heat transfer piece can cover all the lower port of vertical through hole；Under the vertical through hole of metal heat transfer piece sealing-in
The mode of port is：In the uncured PDMS of the top coating of metal heat transfer piece, metal heat transfer piece is then affixed on vertical through hole
Lower port on, then in 100 DEG C of constant temperature oven solidify, form the detection zone of upper end open and lower end closed.
To make the bottom of metal heat transfer post not contacted with the working surface of piezoelectric substrate, i.e., need to ensure the bottom of metal heat transfer post
Portion is slightly above the working surface of piezoelectric substrate, need to so ensure that the height of metal heat transfer pole is omited with the thickness sum of metal heat transfer piece
Less than the height of PDMS cushion blocks, piezoelectric substrate described in the distance from bottom of described metal heat transfer post can be made in actual design
0.2~0.5 millimeter of working surface.
The device also includes signal generation apparatus, multichannel toggle switch, the pcb board for producing RF electric signals, described
Signal generation apparatus are by for producing the signal generator of RF electric signals and being connected with the output end of described signal generator
Power amplifier is constituted, and described pcb board is connected on the lower surface of described piezoelectric substrate, is provided with described pcb board
Terminal pin corresponding with the interdigital transducer described in each, the remittance of the corresponding described interdigital transducer of described terminal pin
Stream bar connection, described terminal pin is connected by described multichannel toggle switch with the output end of described power amplifier, institute
RF electric signals loading after the amplification that the multichannel toggle switch stated selectively exports the output end of described power amplifier
In on the interdigital transducer described in or two it is symmetrical described in interdigital transducer on.Here, power amplifier with draw
One multichannel toggle switch is set between stitch, using after the amplification that multichannel toggle switch selectively exports power amplifier
RF electric signals are loaded on any one interdigital transducer that oil phase microfluid is transported to the lower section of any detection zone, or are loaded into
Any oil phase mirco-fluidic radiation sound on symmetrical interdigital transducer to be pointed between two symmetrical interdigital transducers
Surface wave makes oil phase microfluid produce heat.
A kind of method of micro- liquid heating in detection zone of array chip, it is characterised in that in a kind of above-mentioned array core
Realized on the device of micro- liquid heating in the detection zone of piece, it is comprised the following steps：
1. signal generator and power amplifier, connection power amplifier and multichannel toggle switch are connected, connection multichannel is dialled
Code switch and terminal pin, the busbar of the corresponding interdigital transducer of connecting lead wire pin；
2. oil phase microfluid is positioned on the middle section of the working surface of piezoelectric substrate；Micro- reaction solution sample introduction is arrived it
In in one or more detection zones；
3. enabling signal generator and power amplifier, signal generator output RF electric signals, and transmit RF electric signals to
Power amplifier；
4. successively it is loaded into by the RF electric signals after the amplification that multichannel toggle switch exports power amplifier different
On interdigital transducer, the surface acoustic wave that different interdigital transducers is successively excited is acted on and makes oil phase microfluid on oil phase microfluid
Move at the metal heat transfer post being placed with below the detection zone of micro- reaction solution；Then multichannel toggle switch is passed through by power amplification
RF electric signals after the amplification of device output are loaded on the interdigital transducer of the symmetrical both sides of oil phase microfluid, and the two are right
The surface acoustic wave that the interdigital transducer of title is excited makes oil phase microfluid produce heat while acting on oil phase microfluid, oil phase is micro-
The heat that fluid is produced reaches the detection zone for being placed with micro- reaction solution after the metal heat transfer post heat conduction that oil phase microfluid is contacted, real
Now in the detection zone micro- reaction solution heating；
If 5. having sample introduction in multiple detection zones has micro- reaction solution, repeat step process 4., to complete each detection zone
The heating of interior micro- reaction solution；
6. shutdown signal generator and power amplifier.
Compared with prior art, the advantage of the invention is that：
1) device is acted on oil phase microfluid first with the surface acoustic wave that different interdigital transducers is successively excited, and is made
Oil phase microfluid is moved on the working surface of piezoelectric substrate, until arrival sample introduction has the metal below the detection zone of micro- reaction solution
At heat transfer pole；The surface acoustic wave that the interdigital transducer positioned at the symmetrical both sides of oil phase microfluid is excited is recycled while acting on oil
On phase microfluid, oil phase microfluid is set to produce heat, heat is through metal heat transfer post and metal heat transfer piece heat conduction in detection zone
Micro- reaction solution heating；The heating of micro- reaction solution in any one detection zone in array chip can be realized using surface acoustic wave,
The flexibility of micro- reaction solution heating and accuracy in the particular detection area of array chip are improve, and it is direct without surface acoustic wave
Radiate into the micro- reaction solution for being heated, it is to avoid active shadow of the surface acoustic wave direct radiation to biomolecule in micro- reaction solution
Ring.
2) device is each in the peripheral regions of the working surface of piezoelectric substrate makes row's interdigital transducer, in central area
An array chip is set up on domain, and oil phase microfluid is also positioned on middle section, is not only simple in structure, and volume
Small, it is easy to integrated, can be used for piezoelectricity microflow analysis chip carries out miniflow biochemical analysis.
Brief description of the drawings
Fig. 1 is the structural representation of the device of micro- liquid heating in the detection zone of array chip of the invention.
Specific embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
Embodiment one：
The device of micro- liquid heating in a kind of detection zone of array chip that the present embodiment is proposed, as illustrated, it includes
Piezoelectric substrate 1, the upper surface of piezoelectric substrate 1 is working surface, using integrated in four side regions of the working surface of piezoelectric substrate 1
Circuit technology respectively makes the interdigital transducer 2 for having a row for exciting surface acoustic wave, the central area of the working surface of piezoelectric substrate 1
Domain restocking is provided with an array chip 3, and array chip 3 is included in the PDMS condensates 31 of rectangular structure, PDMS polymerizations
The vertical through hole 32 of multiple arranged with array way, sealing-in in the lower port of vertical through hole 32 are offered by puncher on body 31
There is metal heat transfer piece 33 to form the detection zone for placing micro- reaction solution of upper end open and lower end closed, due under detection zone
End seal is closed, therefore micro- reaction solution will not flow out detection zone, and the bottom of metal heat transfer piece 33 is connected with metal heat transfer with welding manner
Post 34, is respectively provided with a PDMS cushion block 4 on the corner of the bottom of PDMS condensates 31, PDMS condensates 31 pass through four PDMS
Cushion block 4 is padded to be set up on the middle section of the working surface of piezoelectric substrate 1, and makes bottom and the piezoelectricity of metal heat transfer post 34
The working surface of substrate 1 is not in contact with each other, and oil phase microfluid 9 is placed with the middle section of the working surface of piezoelectric substrate 1, interdigital
The surface acoustic wave that transducer 2 is excited is acted on oil phase microfluid 9, and the heat that oil phase microfluid 9 is produced is through metal heat transfer post 34
With the micro- reaction solution in the heat conduction of metal heat transfer piece 33 heating detection zone.
In the present embodiment, in the row's interdigital transducer 2 for being made in every side region of the working surface of piezoelectric substrate 1,
Spacing distance between two neighboring interdigital transducer 2 is 0.4~0.6 millimeter, such as in practice by two neighboring interdigital transducer 2
Between spacing distance be 0.5 millimeter, limit the spacing distance between two neighboring interdigital transducer 2, be in order to ensure interdigital
The surface acoustic wave that transducer 2 is excited can act on the oil phase microfluid 9 on the middle section of the working surface of piezoelectric substrate 1
On, oil phase microfluid 9 is moved to the underface of any one detection zone of array chip 3 such that it is able to utilize sound table
Face ripple and oil phase microfluid 9 realize the heating to the micro- reaction solution in the detection zone；If between two neighboring interdigital transducer 2
Spacing distance it is too big, then do not reach above-mentioned purpose very likely；If the spacing distance between two neighboring interdigital transducer 2
Too small, then the quantity of interdigital transducer 2 can increase, and technology difficulty can also increase, so as to cost will be caused to increase.
In the present embodiment, the thickness of PDMS condensates 31 is that the height of vertical through hole 32 is 2~3 millimeters, is such as designed as
2.5 millimeters.Because the height of vertical through hole 32 is relevant with the amount of micro- reaction solution, thus PDMS condensates 31 too it is thick need not, and
To reach capacity certain in detection zone if too thin, being then relatively large in diameter for vertical through hole 32 is required, and so in array
The area of array chip must be increased in chip in the case of same detection number, the area of piezoelectric substrate is also increased, carried significantly
The device cost of the device high.
In the present embodiment, the size of metal heat transfer piece 33 is more than the aperture of vertical through hole 32, to cover all vertically
The lower port of through hole 32.Can be by the aperture 1 being designed and sized to more than vertical through hole 32 of metal heat transfer piece 33 in actual design
~3 millimeters such as 2 millimeters, to ensure that metal heat transfer piece 33 can cover all the lower port of vertical through hole 32；Metal heat transfer piece
The mode of the lower port of the vertical through hole 32 of 33 sealing-ins is：In the uncured PDMS of the top coating of metal heat transfer piece 33, then will
Metal heat transfer piece 33 is affixed in the lower port of vertical through hole 32, then is solidified in 100 DEG C of constant temperature oven, form upper end open and
The detection zone of lower end closed.
In the present embodiment, for the bottom for making metal heat transfer post 34 does not contact with the working surface of piezoelectric substrate 1, that is, need really
Insurance money belongs to the working surface of the bottom slightly above piezoelectric substrate 1 of heat transfer pole 34, need to so ensure the height of metal heat transfer pole 34 with
The thickness sum of metal heat transfer piece 33 is slightly less than the height of PDMS cushion blocks 4, and the bottom of metal heat transfer post 34 can be made in actual design
Portion is such as designed as 0.3 millimeter apart from 0.2~0.5 millimeter of the working surface of piezoelectric substrate 1.
In the present embodiment, the device also includes signal generation apparatus 8, the multichannel toggle switch for producing RF electric signals
7th, pcb board 6, signal generation apparatus 8 are by for producing the signal generator 81 of RF electric signals and output with signal generator 81
The power amplifier 82 of connection is held to constitute, pcb board 6 is connected on the lower surface of piezoelectric substrate 1, is provided with and each on pcb board 6
The corresponding terminal pin 61 of interdigital transducer 2, the remittance that terminal pin 61 passes through conductive silver glue and the corresponding interdigital transducer 2 of filamentary silver
Stream bar 21 is connected, and terminal pin 61 is connected by multichannel toggle switch 7 with the output end of power amplifier 82, multichannel toggle switch 7
RF electric signals after the amplification that selectively output end of power amplifier 82 is exported load on an interdigital transducer 2 or
On two symmetrical interdigital transducers 2.Here, setting a multichannel toggle switch between power amplifier 82 and terminal pin 61
7, it is loaded into any one using the RF electric signals after the amplification that multichannel toggle switch 7 selectively exports power amplifier 82 interdigital
So that oil phase microfluid 9 to be transported to the lower section of any detection zone on transducer 2, or it is loaded into any to symmetrical interdigital transducer
Oil phase microfluid 9 is made with the radiation surface acoustic wave of oil phase microfluid 9 being pointed between two symmetrical interdigital transducers 2 on 2
Produce heat.
In the present embodiment, piezoelectric substrate 1 can be using the slightly larger piezoelectric substrate of electromechanical coupling factor, basic desirable electromechanics coupling
Piezoelectric substrate of the syzygy number more than 5.5%, such as 1280-YX LiNbO3Piezoelectric substrate；Interdigital transducer 2 uses prior art；
PDMS condensates 31 and PDMS cushion blocks 4 are made using dimethyl silicone polymer (PDMS)；Oil phase microfluid 9 can be paraffin
Oil etc.；Signal generator 81, power amplifier 82, multichannel toggle switch 7, pcb board 6 use prior art, and pcb board 6 also may be used
Substituted by other existing substrates that can fix wire；The bottom of PDMS cushion blocks 4 can be affixed on piezoelectricity by uncured PDMS
On the working surface of substrate 1, and it solidify to form one.
Embodiment two：
The method of micro- liquid heating in a kind of detection zone of array chip that the present embodiment is proposed, it is given in embodiment one
A kind of array chip detection zone in the heating of micro- liquid device on realize, it is comprised the following steps：
1. connection signal generator 81 and power amplifier 82, connect power amplifier 82 and multichannel toggle switch 7, connection
Multichannel toggle switch 7 and terminal pin 61, the busbar 21 of the corresponding interdigital transducer 2 of connecting lead wire pin 61.
2. oil phase microfluid 9 is positioned on the middle section of the working surface of piezoelectric substrate 1；Micro- reaction solution sample introduction is arrived
In wherein one or more detection zones.
3. enabling signal generator 81 and power amplifier 82, the output RF electric signals of signal generator 81, and transmit RF electricity
Signal is to power amplifier 82.
4. by RF electric signals after the amplification that multichannel toggle switch 7 exports power amplifier 82 (power is 31~
33dBm) successively it is loaded on different interdigital transducers 2, the surface acoustic wave that the different priorities of interdigital transducer 2 is excited is acted on
Oil phase microfluid 9 is set to move at the metal heat transfer post 34 being placed with below the detection zone of micro- reaction solution on oil phase microfluid 9；So
It is loaded into positioned at oil phase microfluid 9 by the RF electric signals after the amplification that multichannel toggle switch 7 exports power amplifier 82 afterwards
Symmetrical both sides interdigital transducer 2 on, the surface acoustic wave that the two symmetrical interdigital transducers 2 are excited is while act on oil phase
Oil phase microfluid 9 is set to produce heat, the heat that oil phase microfluid 9 is produced to be passed through the metal that oil phase microfluid 9 is contacted on microfluid 9
The detection zone for being placed with micro- reaction solution is reached after the heat conduction of plume 34, the heating of micro- reaction solution in the detection zone is realized.
The surface acoustic wave that interdigital transducer 2 positioned at the symmetrical both sides of oil phase microfluid 9 is excited need to be while act on oil phase
On microfluid 9, so while oil phase microfluid 9 is produced heat, oil phase microfluid 9 is avoided well and is moved；Certainly
If the RF electric signals after the amplification being loaded on interdigital transducer 2 are smaller, even if the sound surface that an interdigital transducer 2 is excited
Ripple acts on and will not also make on oil phase microfluid 9 oil phase microfluid 9 and move, but this also results in the temperature rise of micro- reaction solution
Less.
If 5. having sample introduction in multiple detection zones has micro- reaction solution, repeat step process 4., to complete each detection zone
The heating of interior micro- reaction solution.
6. shutdown signal generator 81 and power amplifier 82.
Claims (7)
1. the device that micro- liquid is heated in a kind of detection zone of array chip, it is characterised in that including piezoelectric substrate, described pressure
The upper surface of electric substrate is working surface, and each making has the row to be used in four side regions of the working surface of described piezoelectric substrate
Excite the interdigital transducer of surface acoustic wave, the middle section restocking of the working surface of described piezoelectric substrate is provided with an array
Chip, described array chip includes PDMS condensates, offers what is arranged with array way on described PDMS condensates
Multiple through holes vertically, are sealed with metal heat transfer piece and form upper end open and lower end closed in the lower port of described vertical through hole
Detection zone for placing micro- reaction solution, the bottom of described metal heat transfer piece is connected with metal heat transfer post, and described PDMS gathers
One PDMS cushion block is respectively installed, described PDMS condensates are by the PDMS cushion blocks described in four on the corner of fit bottom
It is padded to be set up on the middle section of the working surface of described piezoelectric substrate, and make the bottom of described metal heat transfer post with
The working surface of described piezoelectric substrate is not in contact with each other, and oil is placed with the middle section of the working surface of described piezoelectric substrate
Phase microfluid, the surface acoustic wave that described interdigital transducer is excited is acted on described oil phase microfluid, and described oil phase is micro-
The heat that fluid is produced is through micro- in described metal heat transfer post and the described detection zone of described metal heat transfer piece heat conduction heating
Reaction solution.
2. the device that micro- liquid is heated in a kind of detection zone of array chip according to claim 1, it is characterised in that institute
It is two neighboring described in the interdigital transducer described in a row made in every side region of the working surface of the piezoelectric substrate stated
Spacing distance between interdigital transducer is 0.4~0.6 millimeter.
3. the device that micro- liquid is heated in a kind of detection zone of array chip according to claim 1 and 2, it is characterised in that
The polymeric thickness of described PDMS is that the height of described vertical through hole is 2~3 millimeters.
4. the device that micro- liquid is heated in a kind of detection zone of array chip according to claim 3, it is characterised in that institute
The aperture of vertical through hole of the size of the metal heat transfer piece stated more than described in, to cover all the lower end of described vertical through hole
Mouthful.
5. the device that micro- liquid is heated in a kind of detection zone of array chip according to claim 4, it is characterised in that institute
0.2~0.5 millimeter of the working surface of the piezoelectric substrate described in the distance from bottom of the metal heat transfer post stated.
6. the device that micro- liquid is heated in a kind of detection zone of array chip according to claim 1 and 2, it is characterised in that
The device also includes signal generation apparatus, multichannel toggle switch, the pcb board for producing RF electric signals, and described signal occurs
Device is by the power amplification for producing the signal generator of RF electric signals and be connected with the output end of described signal generator
Device is constituted, and described pcb board is connected on the lower surface of described piezoelectric substrate, is provided with described pcb board and each institute
The corresponding terminal pin of interdigital transducer stated, the busbar of the corresponding described interdigital transducer of described terminal pin connects
Connect, described terminal pin is connected by described multichannel toggle switch with the output end of described power amplifier, and described is more
RF electric signals after the amplification that road toggle switch selectively exports the output end of described power amplifier load on one
On described interdigital transducer or on two symmetrical described interdigital transducers.
7. a kind of method that micro- liquid is heated in detection zone of array chip, it is characterised in that in the one kind described in claim 6
Realized on the device of micro- liquid heating in the detection zone of array chip, it is comprised the following steps：
1. signal generator and power amplifier, connection power amplifier and multichannel toggle switch are connected, connection multichannel dial-up is opened
Close and terminal pin, the busbar of the corresponding interdigital transducer of connecting lead wire pin；
2. oil phase microfluid is positioned on the middle section of the working surface of piezoelectric substrate；By micro- reaction solution sample introduction to wherein
In individual or multiple detection zones；
3. enabling signal generator and power amplifier, signal generator output RF electric signals, and RF electric signals are transmitted to power
Amplifier；
4. successively it is loaded into by RF electric signals after the amplification that multichannel toggle switch exports power amplifier different interdigital
On transducer, the surface acoustic wave that different interdigital transducers is successively excited is acted on and makes oil phase micro-fluid movement on oil phase microfluid
To the metal heat transfer post being placed with below the detection zone of micro- reaction solution；Then it is by multichannel toggle switch that power amplifier is defeated
RF electric signals after the amplification for going out are loaded on the interdigital transducer of the symmetrical both sides of oil phase microfluid, and the two are symmetrical
The surface acoustic wave that interdigital transducer is excited makes oil phase microfluid produce heat, oil phase microfluid while acting on oil phase microfluid
The heat of generation reaches the detection zone for being placed with micro- reaction solution after the metal heat transfer post heat conduction that oil phase microfluid is contacted, and realizing should
The heating of micro- reaction solution in detection zone；
If 5. having sample introduction in multiple detection zones has micro- reaction solution, repeat step process 4. is micro- in each detection zone to complete
The heating of reaction solution；
6. shutdown signal generator and power amplifier.
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