CN106822998A - Promote jawbone regrown material and application - Google Patents
Promote jawbone regrown material and application Download PDFInfo
- Publication number
- CN106822998A CN106822998A CN201710095495.4A CN201710095495A CN106822998A CN 106822998 A CN106822998 A CN 106822998A CN 201710095495 A CN201710095495 A CN 201710095495A CN 106822998 A CN106822998 A CN 106822998A
- Authority
- CN
- China
- Prior art keywords
- bone
- jawbone
- regrown material
- antler polypeptide
- regrown
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3608—Bone, e.g. demineralised bone matrix [DBM], bone powder
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
Abstract
A kind of promotion jawbone regrown material and application of the invention, the regrown material is antler polypeptide composite inorganic ox bone, using this kind of material, can accelerate osteanagenesis, so as to shorten the therapeutic process of Dental implantion.
Description
Technical field
The present invention relates to teeth repairing material technical field, jawbone regrown material and application are promoted more particularly, to one kind.
Background technology
Tooth-planting is in the jawbone of the artificial tooth root implantation missing tooth patient for making titanium alloy or other materials
And a kind of technology of artificial tooth is made thereon.Tooth-planting can well recover the masticatory function of patient, increasingly by tooth
The welcome of deletion patients.In order to promote Osseointegrated implants and improve the success rate of plantation, there is many modified to planting body at present
Method.
First it is physical modification, refers mainly to the change of implant surface structure, physical modification can increases the hydrophilic of planting body
Property and surface area, the surface that is formed is micro-coarse structured, and to be conducive to being formed between implant surface and bone tissue machinery sealed, but
Physical modification is limited to the facilitation of Osseointegrated implants, is only suitable for the basis being modified as implant surface.
Next to that planting body biological modification is more and more interested to researchers, it is mainly by wrapping up or spraying
Specific cell, albumen or siRNA etc. are applied into implant surface etc. mode to change the micro-loop of Osseointegrated implants
Border, promotes cell proliferation and differentiation and the sclerotin secretion with osteogenic action, with progress of research, it has also been realised that being given birth to
Thing is modified the problems such as there is also shorter bioactivator half-life period, somewhat expensive.
It is chemical modification to have again, refers mainly to change the chemical composition of implant surface, and bone biomimetic material is applied to
Implant surface, makes planting body have good biocompatibility and then preferably promotes Osseointegrated implants, but in planting body bone
With reference to after, the catabolite that chemical modification there is also biomimetic material may have a negative impact to Osseointegrated implants
Shortcoming.
Constantly improve and the development of theoretical and technology are prepared with material surface coating, titanium implantses process for modifying surface has
Fast development, this makes titanium implantses biocompatibility obtain further raising.However, Long-term clinical tracking and research hair
Existing, the main cause that implant failure is caused at this stage is that implant surfaces bioactivity is not ideal enough, causes sclerous tissues
Implant is more long with the Integrated implant time, makes the course for the treatment of elongated.
Thus the synosteosis of planting body needs to be further improved by other approach.
The content of the invention
It is an object of the invention to provide one kind promotion jawbone regrown material, the regrown material can accelerate the bone of planting body
With reference to shortening treatment time.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that：
One kind promotes jawbone regrown material, and the regrown material is antler polypeptide composite inorganic ox bone.
Preferably, the amino acid sequence of the antler polypeptide is E-P-T-V-L-D-E-V-C-L-A-H-G-P.
Preferably, the regrown material wraps up inorganic bone meal and is obtained by the aqueous solution of antler polypeptide；The antler polypeptide water
The concentration of solution is 100 μ g/mL；The Bio-oss is graininess, and the granularity of the particle is 0.25-1mm, the particle
Average quality is 0.25g.
Preferably, the antler polypeptide aqueous solution and the amount ratio of the inorganic bone are the antler polypeptide aqueous solution and institute
It is the 1ml antler polypeptides aqueous solution and the inorganic bone meal mixing of 0.25g-0.5g to state the amount ratio of inorganic bone.
Above-mentioned promotes application of the jawbone regrown material in dental implant surface is modified.
Application of the above-mentioned promotion jawbone regrown material in Dental implantion bone defect healing.
Preferably, the regrown material is integrated with the bone tissue around tooth implant.
Preferably, the tooth implant is metal material, ceramic material or macromolecular material.
Preferably, the tooth implant is metal material.
Preferably, the metal material is titanium or titanium alloy.
Beneficial effects of the present invention are as follows：
Antler polypeptide has the effect that inducing osteoblast grows, with reference to the support effect of the guiding regeneration of Bio-oss,
The invention can very well solve the problems, such as the Cranial defect in Dental implantion reparation.
Brief description of the drawings
In order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art, below will be to specific
The accompanying drawing to be used needed for implementation method or description of the prior art is briefly described, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 a are the amount that 48h OS-732 cells (Saos-2 cells) secrete ALP；
Fig. 1 b are the propagation of CCK8 detection method 12h OS-732 cells (Saos-2 cells)；
The migration of cell when Fig. 2 a are blank group OS-732 cells (Saos-2 cells) 0h；
Fig. 2 b are cell migration situation of the blank group OS-732 cells (Saos-2 cells) after 48h is cultivated；
Cell migration when Fig. 3 a are experimental group OS-732 cells (Saos-2 cells) 0h；
Fig. 3 b are cell migration situation of the experimental group OS-732 cells (Saos-2 cells) after 48h is cultivated；
Fig. 4 a are calcium tubercle formational situation after blank group 48h；
Fig. 4 b are calcium tubercle formational situation after experimental group 48h；
Fig. 5 a are experimental group Oesteoblast growth situation；
Fig. 5 b are control group Oesteoblast growth situation.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Tooth-implanting is one of best recovery technique of present absence of tooth, and main material is two parts of planting body and corona
Composition.Tooth-implanting is advantageous in that：Tooth-implanting will not be made as that may be fallen off when normal activity artificial tooth is at table or is spoken
Into the danger for blocking tracheae or esophagus；Artificial tooth root is rooted in alveolar bone, to the functional sexual stimulus of alveolar bone, can protect alveolus
Bone structure, it is to avoid its atrophy；The tooth of health can be fully retained, it is not necessary to grind off the tooth of neighbouring health；Implant denture leads to
The fixing device crossed on foundation pile, top dentures are fixed, and have good maintenance and stabilization to increase comfort, are had well
Masticatory force；Implant tooth can have the life-span very long；It is aesthetic in appearance in addition, when speaking facial expression general as natural teeth
As originally equally natural.
Tooth-planting material refer to be implanted in oromaxillo-facial region tissue substitute the tooth of missing, the bone tissue of defect and
The correction of bone tissue deformity, to recover the physiology profile of patient and the biomaterial of function.In planting body implantation human body, material list
The physicochemical properties and surface texture in face can significant impact cell stick, stretch, growing, breeding and cell activity
And guiding.Titanium or titanium alloy have good mechanical performance and certain biocompatibility cause its in clinical practice extensively, but
Be that titanium is bio-inert material, itself still do not possess biological function, and in vivo after long service surface is corroded, trip
Separating out the metal ion for coming can make it go bad into organizing.In order to improve the corrosion resistance and bioactivity of planting body, induction with
Bone tissue forms biological combination, surface is carried out to planting body and is modified, and is a kind of further mode for improving planting body effect.
After tooth is lacked because of reasons such as wound, inflammation, tumour, periodontosis, often with the taken in excess of alveolar bone, cause to plant
The Bone mineral change of growing area, is extremely restricted Dental implantion reparation.How to solve the problems, such as that growing area Bone mineral change is always one
Individual focus and difficult point.At present, the method that bone regeneration around implant Bone mineral change is clinically solved both at home and abroad is typically in Cranial defect area
It is interior to fill bone grafting material and improve implant surface treatment technology to guide new bone growth.But, in terms of induced osteogenesis
There is no perfect method, the inventors found that using antler polypeptide composite inorganic ox bone as induced osteogenesis the factor, can
Accelerate the regeneration of bone, better method and thinking are found to solve dental implant bone defect.
Ox bone based on inorganic constituents, about 86%, the Mg3 (P04) 2 about 1% of wherein Ca3 (PO4) 2, other calcium salts about 7%,
Chlorine about 0.2%, fluorine about 0.3%.Organic principle is multiple proteins, and one of which name ossein (Ossein) person constitutes network
Shape is distributed in bone, is such as dissolved inorganic matter with hydrochloric acid, then ossein keeps original bone shape in spongy.Ossein one
Such as the collagen in skin, boiled altogether with water, then give birth to gelatin.The fat content of ox bone, it is different because of the species of bone, about 12~20%.Structure
Into the aliphatic acid of fat, mainly palmitic acid, stearic acid and oleic acid, but also containing a small amount of linoleic acid etc..
Antler polypeptide can promote transmission to heal, the propagation of antler polypeptide energy obvious stimulation cartilage cell and osteoblast,
Its proliferation function has obvious dose-effect relationship and without species specificity, and antler polypeptide has acceleration repair to defect of skin, right
Fracture be obviously promoted Healing, it be by promote bone, chondrocyte proliferation and promote poroma in ossein accumulation and
Calcium deposition and accelerating union of bone fracture.Antler polypeptide has obvious facilitation, energy to epidermal cell and fibroblast proliferation
Accelerate skin wound healing；Antler polypeptide is cut by promoting the influence of hepatocyte growth and strengthen immunity come Acceleration study liver
Except the liver regeneration of mouse.Antler polypeptide extract can accelerate the healing of skin wound.
Antler polypeptide has obvious inhibitory action to various Acute and chronic inflammations, and mechanism is antler polypeptide to capillary
The increase of permeability has obvious inhibitory action.
Embodiment 1
Antler polypeptide composite inorganic bovine bone powder compares the influence of cell proliferation and cytoactive with blank group.
Experiment in vitro Secondary Culture Saos-2 OS-732 cells, are done with the compound Bio-Oss bone meal of antler polypeptide
In advance, using the specific activity of CCK-8 methods, alkaline phosphatase (ALP) method such as determining detects the propagation and activity of Gegenbaur's cell, and
Compare with blank group, as a result such as Fig. 1 a, shown in 1b.
It is that Saos-2 OS-732 cells are carried out with antler polypeptide composite inorganic bone meal (PAP+Bio-oss) in Fig. 1 a
Intervene the content of the intracellular alkaline phosphatase that 24h compares with blank group (control).
Fig. 1 b are after Saos-2 OS-732 cells antler polypeptide composite inorganic bone meal (PAP+Bio-oss) is intervened
24h blank groups (control), alkaline phosphatase in the supernatant of antler polypeptide (pap) composite inorganic bone meal group (bio-oss)
Content situation.
Comparative result shows that the compound bio-oss bone meal of antler polypeptide can promote Osteoblast migration, breed.
Embodiment 2
Promote the synthesis and application of jawbone regrown material
Promotion jawbone regrown material in this implementation is antler polypeptide composite inorganic ox bone,
The antler polypeptide for using is a kind of active small peptide, and its amino acid sequence is E-P-T-V-L-D-E-V-C-L-
A-H-G-P；By Shanghai gill biochemistry, Co., Ltd synthesizes.
Bio-oss is ordinary commercial products；Selected Bio-oss is graininess, and the granularity of particle is in 0.25mm-
1mm, the average quality of particle is 0.25g, and the average external volume of particle is 0.5mL；
Promote the preparation process of jawbone regrown material as follows：
Antler polypeptide (PAP) is dissolved in tri-distilled water, adjustment concentration is 100 μ g/mL, and stirs mixing with Bio-oss bone meal；
Calculate by volume, the volume energy of antler polypeptide liquid wraps up inorganic bone meal.The antler polypeptide that will be prepared and inorganic ox
The step of composite of bone covers implant surface is as follows：
Accumulated according to dental implant surface, calculate the mixeding liquid volume for needing, the mixed liquor that step 1 is prepared is smeared and is covered in
Implant surface；Tooth implant chooses the planting body of titanium matter.
Embodiment 3
To the cell scratch experiment of the promotion jawbone regrown material in embodiment 2
Experimental cell：Saos-2 cells；
Complete medium：10%FBS, 90%McCoy's 5A culture mediums, 100 μ g/mL penicillin, 100 μ g/mL strepto-s
Element；
Experimental technique：By Saos-2 cell culture in the McCoy's 5A culture mediums containing 10% hyclone, 100 are added
The streptomysin of the penicillin and 100 μ of μ/mL/mL, is placed in and contains 5%CO2, cultivated in 37 DEG C of incubator；
Take the logarithm growth period each group cell, distinguish after conventional digestion during equivalent adds 12 orifice plates equipped with titanium sheet, add per hole
Enter about 1.1*105Individual cell；
Cell is randomly divided into 2 groups：Blank control group, antler polypeptide and Bio-oss complex group；
It is put into 37 DEG C of 5%CO2Incubator, is paved with bottom hole after 24h, ruler is compared with pipette tips, perpendicular to bottom hole cut, uses
PBS washes cell 3 times, the cell under removal stroke, and antler polypeptide is added with Bio-oss complex group and promotes jawbone regrown material, blank
Control group then adds completely isometric culture medium；
It is put into 37 DEG C of 5%CO2Incubator continues to cultivate；
Sampled in the 0th and 48 hour after intervention, taken pictures；
Result such as Fig. 2 a, 2b, 3a, shown in 3b,
This experiment with Saos-2 Gegenbaur's cells and titanium sheet, in simulating oral implant rehabilitation, by the tooth of titaniferous material matrix
Planting body plants the process in alveolar bone.Experimental group can be seen that by Fig. 3 b and intervene 48h through antler polypeptide composite inorganic ox bone
Afterwards, cell has obvious migration, and migrating cell has been completely covered scored area substantially；And the cell after the control group 48h of Fig. 2 b
Then migrate slow.
Embodiment 4
To the cytoactive ALP test experiences of the promotion jawbone composite recycled material in embodiment 2
Experimental cell：Saos-2 cells；
Complete medium：10%FBS, 90%McCoy's 5A culture mediums, 100 μ g/mL penicillin, 100 μ g/mL strepto-s
Element；
Experimental technique：By Saos-2 cell culture in the McCoy's 5A culture mediums containing 10% hyclone, add
The penicillin of 100u/ml and the streptomysin of 100u/ml, are placed in and contain 5%CO2, cultivated in 37 DEG C of incubator；
Take the logarithm growth period each group cell, be divided in after conventional digestion in 2 blake bottles, 2 blake bottles are respectively blank
Control group and antler polypeptide composite inorganic ox bone group；
Antler polypeptide composite inorganic ox bone group is added and promotes jawbone regrown material, and blank control group then adds completely isometric
Culture medium；
Continue 5%CO2, after 37 DEG C of culture 24h, conventional digestion；
It is respectively charged into 15mL centrifuge tubes, takes culture medium supernatant and be separately added into 96 orifice plates, adds ALP reagents, thermostatted water
ELIASA mensuration absorbance value is used after bath；Understood after antler polypeptide is processed by experimental result, the propagation of Saos-2 cells
Activity is significantly improved.
Experiment above shows that antler polypeptide can promote activity and the migration of Gegenbaur's cell, imply that antler polypeptide can
Promote in oral implant rehabilitation, the synosteosis speed of tooth implant shortens treatment course.
Embodiment 5
Gegenbaur's cell Alizarin red staining method experiment to the promotion jawbone regrown material in embodiment 2
Experimental cell：Saos-2 cells；
Complete medium：10%FBS, 90%McCoy's 5A culture mediums, 100 μ g/mL penicillin, 100 μ g/mL strepto-s
Element；
Experimental technique：By Saos-2 cell culture in the McCoy's 5A culture mediums containing 10% hyclone, add
The penicillin of 100u/ml and the streptomysin of 100u/ml, are placed in and contain 5%CO2, cultivated in 37 DEG C of incubator；
Take the logarithm growth period each group cell, be inoculated with after conventional digestion into 12 orifice plates, cell is randomly divided into 2 in 12 orifice plates
Group：Blank control group, antler polypeptide composite inorganic ox bone group；
Antler polypeptide composite inorganic ox bone group is added and promotes jawbone regrown material, and blank control group then adds completely isometric
Culture medium；
After 48h, culture medium, 95% alcohol fixation 15min are suctioned out；Alizarin red staining 5min, and in electric Microscopic observation calcium knot
Section formational situation.
Result is as shown in Figs. 4a and 4b,
Fig. 4 a are calcium tubercle formational situation after blank control group 48h；
Fig. 4 b are calcium tubercle formational situation after experimental group 48h.
Embodiment 6
Zoopery
12 Xishuangbanna miniature pigs are randomly divided into experimental group and control group, wherein experimental group 6, control group 6,
1cmx1cm wounds are manufactured on the alveoli dentales mandibulae bone of miniature pig；The Bio-oss bone meal of the compound PAP of experimental group implantation, control group exists
Add tri-distilled water in wound；Experimental group and control group miniature pig are put to death respectively after being implanted into 6 weeks, and cuts mandibular at wound, cut
Piece, Toluidine blue staining；Fig. 5 a are experimental group Oesteoblast growth situation, and Fig. 5 b are control group Oesteoblast growth situation, from
It can be seen that osteoblast number is high compared with control group at experimental group Cranial defect in figure.
Embodiment 7
Promotion jawbone regrown material prepared by embodiment 3 is covered on the tooth implant of titanium matter and by tooth implant kind
Plant the process in human mouth
The principle of tooth-implanting is to be implanted into tooth-implanting body (emulation is at all) in alveolar bone for tooth-implanting, is then pacified at an upper portion thereof
Dress base station and porcelain corona.No matter be implanted into one or several teeth, in addition upper lower jaw whole teeth, plant tooth process and be substantially all
It is the same.
Tooth-implanting includes seven processes,
1. Preoperative Method
Doctor patient communication before treatment, understands expectation and the hope of patient；
Exclude pathological state, infection；
To the tooth without therapeutic value, the time of pulling out is drafted as the case may be；
Provisional prosthesis is adjust and reform or made, makes it that there is appropriate vertical range, Arch width and jaw plane, one can be completed
Fixed function and the certain aesthetic requirement of maintenance；
2. examination of mouth
Doing tooth-implanting operation will first check whether periodontal has disease, if it find that periodontal has disease, it is necessary to first treat, it is impossible to
The tooth for the treatment of is then pulled out；
Before tooth-implanting operation, it will usually do operation before a plantation, including：Soft tissue plasty, alveoloplasty, tooth
The operation that groove bone increment, Maxillary Sinus etc. cannot be carried out with the planting body implantation same period
3. tooth-implanting operation guide plate is made
According to plantation body position, design determines the scheme of plantation nest；
4. plantation nest is prepared
By the template for being pre-designed making, the planting body and corresponding series of the bone amount selection suitable length according to alveolar bone
Bore, using the quick drill of Dental implantion machine, with a large amount of normal saline flushings, first positioned with round bur and drilled, followed by with splitting brills, navigate brill
Progressively reaming, then expands suitable for reading, rinses wound；
5. screw thread is prepared
Use instead and bore at a slow speed, equally lowered the temperature with a large amount of normal saline flushings, the screw thread on plantation nest bone wall is prepared with screw tap；
6. Implant
Planting body is slowly implanted into the plantation nest got ready and is carefully reinforced with special instrument and screwed, make planting body top
Edge is equal with bone face.
7. sewing-up cut；
8. corona is taken
After planting nail and the tight healing of alveolar bone, the full mistake of whole tooth-implanting operation is just completed after installation porcelain corona
Journey.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
<110>Kunming Medical University
<120>Promote jawbone regrown material and application
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 14
<212> PRT
<213>Artificial sequence
<400> 1
Glu Pro Thr Val Leu Asp Glu Val Cys Leu Ala His Gly Pro
Claims (9)
1. it is a kind of to promote jawbone regrown material, it is characterised in that the regrown material is antler polypeptide composite inorganic ox bone.
2. it is according to claim 1 to promote jawbone regrown material, it is characterised in that the amino acid sequence of the antler polypeptide
It is E-P-T-V-L-D-E-V-C-L-A-H-G-P.
3. it is according to claim 1 to promote jawbone regrown material, it is characterised in that the regrown material is by antler polypeptide
The aqueous solution wraps up inorganic bone meal and is obtained；The concentration of the antler polypeptide aqueous solution is 100 μ g/mL；The Bio-oss is particle
Shape, the granularity of the particle is 0.25-1mm, and the average quality of the particle is 0.25g.
4. it is according to claim 3 promotion jawbone regrown material, it is characterised in that the antler polypeptide aqueous solution with it is described
The amount ratio of inorganic bone is the 1ml antler polypeptides aqueous solution and the inorganic bone meal mixing of 0.25g-0.5g.
5. the application for promoting jawbone regrown material in Dental implantion bone defect healing as claimed in claim 1.
6. application according to claim 5, it is characterised in that the regrown material promotes repairing for Dental implantion repairing bone defect
Multiple and quickening planting body is combined with surrounding bone.
7. the application according to claim 5 or 6, it is characterised in that the tooth implant be metal material, ceramic material or
Macromolecular material.
8. application according to claim 7, it is characterised in that the tooth implant is metal material.
9. application according to claim 8, it is characterised in that the metal material is titanium or titanium alloy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710095495.4A CN106822998A (en) | 2017-02-22 | 2017-02-22 | Promote jawbone regrown material and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710095495.4A CN106822998A (en) | 2017-02-22 | 2017-02-22 | Promote jawbone regrown material and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106822998A true CN106822998A (en) | 2017-06-13 |
Family
ID=59133191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710095495.4A Pending CN106822998A (en) | 2017-02-22 | 2017-02-22 | Promote jawbone regrown material and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106822998A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147809A (en) * | 2007-10-30 | 2008-03-26 | 王喜文 | Method for preparing bone grafting material with pilose antler and deer bone as material and the bone grafting material prepared by the method |
| CN102225967A (en) * | 2011-04-25 | 2011-10-26 | 广州皇莎化妆品有限公司 | Method of preparing pilose antler polypeptide with pure natural, high activity and high purity |
| CN103041452A (en) * | 2011-10-13 | 2013-04-17 | 中国科学院理化技术研究所 | Porous bone repair framework material of gelatin/nano bone-like apatite, and preparation method and application thereof |
| CN103463677A (en) * | 2013-06-04 | 2013-12-25 | 吉林大学 | Magnetic hierarchically-porous nanometer sustained-release bone restoration material, and preparation method and application thereof |
| CN105722536A (en) * | 2013-10-03 | 2016-06-29 | 叉骨公司 | Bone regeneration material and manufacture method thereof |
-
2017
- 2017-02-22 CN CN201710095495.4A patent/CN106822998A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147809A (en) * | 2007-10-30 | 2008-03-26 | 王喜文 | Method for preparing bone grafting material with pilose antler and deer bone as material and the bone grafting material prepared by the method |
| CN102225967A (en) * | 2011-04-25 | 2011-10-26 | 广州皇莎化妆品有限公司 | Method of preparing pilose antler polypeptide with pure natural, high activity and high purity |
| CN103041452A (en) * | 2011-10-13 | 2013-04-17 | 中国科学院理化技术研究所 | Porous bone repair framework material of gelatin/nano bone-like apatite, and preparation method and application thereof |
| CN103463677A (en) * | 2013-06-04 | 2013-12-25 | 吉林大学 | Magnetic hierarchically-porous nanometer sustained-release bone restoration material, and preparation method and application thereof |
| CN105722536A (en) * | 2013-10-03 | 2016-06-29 | 叉骨公司 | Bone regeneration material and manufacture method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Angelo et al. | Biomechanical stability of dental implants in augmented maxillary sites: results of a randomized clinical study with four different biomaterials and PRF and a biological view on guided bone regeneration | |
| Del Corso et al. | The use of leukocyte-and platelet-rich fibrin during immediate postextractive implantation and loading for the esthetic replacement of a fractured maxillary central incisor | |
| Anitua et al. | A lateral approach for sinus elevation using PRGF technology | |
| US20130164707A1 (en) | Devices and methods for enhancing bone growth | |
| TW425279B (en) | Method and device for preparing a dental implant by immersion in a mesenchymal cell culture | |
| CN101579537B (en) | Surface treatment method of tissue-engineered bioactive implant | |
| US10603410B2 (en) | Complex of implant and cultured periodontal ligament cell sheet, method for manufacturing the same, and method for using the same | |
| CN106333753B (en) | The dental implant and preparation method thereof of individuation bionic | |
| EP1106145A2 (en) | Method and apparatus for performing jawbone ridge augmentation | |
| Hao et al. | Effect of umbilical cord mesenchymal stem cell in peri-implant bone defect after immediate implant: an experiment study in beagle dogs | |
| Gu et al. | Effect of low intensity pulsed ultrasound on repairing the periodontal bone of Beagle canines | |
| CN107260340A (en) | The protection structure of Alveolar Bone Defect bone graft area | |
| CN204542414U (en) | A kind of Absorbable rod tooth-planting bone ring being applicable to cheek side or tongue side Alveolar Bone Defect | |
| CN106822998A (en) | Promote jawbone regrown material and application | |
| US20160302892A1 (en) | Supraosseous dental implant | |
| CN207286182U (en) | Repair titanium net-titanium plate complex of large-scale mandibular defect | |
| Huang et al. | Periodontal Ligament Cell Sheet Engineering: A new Possible Strategy to Promote Periodontal Regeneration of Dental Implants. | |
| Horowitz | Current State of Bone Replacement Grafting Materials for Dental Implants. | |
| RU2320285C2 (en) | Method for restoring alveolar jaw bone crest and periodontium tissues having reduced recovery potential | |
| RU177734U1 (en) | RECONSTRUCTIVE IMPLANT FROM LYOPHILIZED ALLOGENIC MATERIAL | |
| Al-Sadah et al. | Osseous Integration After Exenteration | |
| Lazić et al. | Contouring the emergence profile of peri-implant soft tissue by provisionals on implants: Case report | |
| CN105105861B (en) | A kind of bioengineered tooth wears gum system | |
| US11872105B1 (en) | Dental implant device for regeneration of dental pulp and dentin | |
| CN110236710A (en) | A method of promote bone-grafting material to promote postoperative skeletonization outside Dental implantion maxillary sinus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170613 |
|
| RJ01 | Rejection of invention patent application after publication |