CN1068198C - Production of composite preparation of bone morphogenesis protein and collagen particle - Google Patents
Production of composite preparation of bone morphogenesis protein and collagen particle Download PDFInfo
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- CN1068198C CN1068198C CN98125300A CN98125300A CN1068198C CN 1068198 C CN1068198 C CN 1068198C CN 98125300 A CN98125300 A CN 98125300A CN 98125300 A CN98125300 A CN 98125300A CN 1068198 C CN1068198 C CN 1068198C
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- buffer solution
- acetate buffer
- collagen
- morphogenetic protein
- bone morphogenetic
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Abstract
The present invention belongs to the field of biomedical materials, which mainly relates to preparation of a composite preparation form of bone morphogenetic protein and collagen particles. In the method, firstly, collagen is dissolved in an acetic acid buffer solution; in addition, bone morphogenetic protein is added to a mixed solution of guanidine hydrochloride and the acetic acid buffer solution; the two solutions are mixed; after the mixture is stirred and stands for certain time, ethanol is added; immediately, the mixture is centrifugally precipitated; after precipitates are washed and aired, the composite preparation form of bone morphogenetic protein and collagen, which needs to be prepared by the present invention, can be obtained.
Description
The invention belongs to field of biomedical materials.Mainly be applicable to the artificial bone implant into body, promote new bone development growth, quicken physiology and fix.
Bone morphogenetic protein (bone morphogenefic protein, be called for short BMP) be a kind of protein in body factor with unique induced osteogenesis function, in body osteanagenesis and repair process, bring into play pivotal role, knochenbruch is connect fast again, in the orthopaedics and the department of stomatology, have extensively and application prospect.
At present, preparation BMP has two kinds of methods: a kind of is to utilize biochemical extraction method directly to extract mixed type BMP from beast bone such as cattle, horse, pig, sheep, rabbit, Mus or osteosarcoma; Another kind is the people BMP that utilizes the synthetic unitary type of method of gene recombination.Gene recombinant human BMP lures bone or bone active strong, and is higher by 10 than the BMP of biochemical extraction method preparation
3-10
6Doubly.Though BMP has very strong induced osteogenesis activity, during implant into body, it is fast to absorb metabolism, and action time is short, is difficult to give full play to its induced osteogenesis effect; In addition, BMP does not have the support effect when reparation large scale bone is damaged.Therefore, BMP also needs with a certain carrier material compound when clinical practice, just so that himself obtain slow release and support.
Collagen (collagen) is the maximum protein of a kind of content that constitutes the bio-tissue structure; account for the 25-30% of human body protein total amount; its function mainly is to bear load, conjunctive tissue and organ, protection body etc., the effect of assisting transmitting tissue's differentiation and organ to form in the body development process in addition.Difference, collagen according to the polypeptide chain type can divide 5 types, are based on the I Collagen Type VI in vivo.Medical collagen is to extract from Corii Bovis seu Bubali, cattle tendon or Corii Sus domestica, pig tendon.Collagen has good biocompatibility, to a little less than organizing avirulence, non-stimulated, antigenicity, platelet is had higher affinity, can promote cell proliferation, accelerating wound, is one of ideal carrier material of BMP.BMP and collagen is compound, both helped the performance of the induced osteogenesis effect of BMP, the collagen tissue repair is strengthened.
In the prior art, document (" The Journal of Biologi cal Chemistry " vol.265, No.22, PP13198-13205) each bone morphogenetic protein and collagen composite methods are provided, this method is that bone morphogenetic protein is joined in about 6M guanidine hydrochloride, make the guanidine hydrochloride solution that contains bone morphogenetic protein, and then collagen is placed in one, through stirring, mixing, add the ethanol low-temperature precipitation, use alcohol flushing at last, nontoxic airing down, lyophilization is preserved stand-by.The major defect of this method is: (1) does not add acetate buffer solution in preparation process, therefore, can not make BMP and collagen compound effectively; (2) do not add gelatin, granular collagen and BMP precipitate are difficult to modulate molding; (3) do not emphasize the collagen granularity, to increase its bone conduction effect.
Order of the present invention is to provide the manufacture method of simple and effective bone morphogenetic protein of a kind of method and the compound dosage form of collagen particle, obtained compound dosage form can make bone morphogenetic protein obtain slow release in vivo effectively, can give full play to the egg type attitude proteic induced osteogenesis effect takes place.
According to above-mentioned purpose, the present invention is that medium prepares above-mentioned required bone morphogenetic protein and the compound dosage form of collagen particle with guanidine hydrochloride solution and acetate buffer solution.The concrete processing step of its manufacture method is as follows:
(1) preparation collagen acetate buffer solution
Granulous collagen is dissolved in the 50-60 ℃ of acetate buffer solution, soaked 0.3-1.0 hour, the addition of collagen is 0.1-0.6mg/ml in the acetate buffer solution,
The acid-base value PH of acetate buffer solution is 4.5-5.0, and acetate concentration 15-25mM gets the collagen acetate buffer solution thus;
(2) preparation bone morphogenetic protein guanidine hydrochloride-acetate buffer solution adds bone morphogenetic protein in the mixed liquor of guanidine hydrochloride and acetate buffer solution, and the addition of bone morphogenetic protein is the 0.3-0.7mg/ml of guanidine hydrochloride-acetate buffer solution mixed liquor,
The concentration of guanidine hydrochloride is 5-7M,
The acid-base value PH of acetate buffer solution is 4.5-5.0, and concentration is 12-25mM,
The proportioning of guanidine hydrochloride and acetate buffer solution (ml/ml) is (0.5-1.5): (1.5-2.5);
(3) the above-mentioned collagen acetate buffer solution of preparation is respectively mixed with bone morphogenetic protein guanidine hydrochloride-acetate buffer solution mixed liquor, and under 20-30 ℃ of temperature, stir, left standstill afterwards 0.5-2 hour; Both mixed proportions are 1: 1;
(4) ethanol is added in the above-mentioned mixed liquor, behind the vibration mixing, under-50--80 ℃ temperature, preserved 0.5-1.5 hour, carry out centrifugation immediately,
Alcoholic acid addition (ml) is 1.5-2.5 a times of mixed liquor;
(5) with 85% and 100% ethanol precipitate is washed respectively, with 85% alcohol flushing twice, once with 100% alcohol flushing, the nontoxic airing down in flushing back behind the airing, adds 10% gelatin, under 30 ℃ of temperature, granular sludge is modulated molding, after lyophilization, preserve stand-by.
Compared with prior art, the present invention has following advantage:
(1) used medium guanidine hydrochloride solution and acetate buffer solution wide material sources, the cost of raw material is low.
(2) the present invention adopts acetate buffer solution that BMP and collagen are adsorbed mutually, improves its combined efficiency.
(3) the present invention adopts with 50-500 μ m collagen particle and the made compound dosage form of getting of 10% gelatin modulation molding and can make BMP obtain slow release in vivo effectively, gives full play to the induced osteogenesis effect of BMP, strengthens the conduction of area of new bone; In addition, the tissue repair effect of collagen is strengthened, the knitting process is obviously accelerated.Experiment shows, BMP and collagen particle dosage form are put into opposite hole type implantation body, implant in the alveolar bone just had in one month a large amount of bone trabecula tooth implant to the hole in form, and merge with alveolar bone, can prevent the loosening of Dental Implant effectively.
Embodiment
According to 3 batches of bone morphogenetic proteins of manufacture method preparation of the present invention and the compound dosage form of collagen particle.
After getting the raw materials ready, at first a grain collagen being put into temperature is 50-60 ℃ acetate buffer solution, and soaks certain hour, makes it to obtain the collagen acetate buffer solution, and the addition of collagen and acetate buffer solution, concentration and acid-base value are as shown in table 1.
In addition, bone morphogenetic protein is added in the mixed liquor of guanidine hydrochloride and acetate buffer solution.The concentration of guanidine hydrochloride and acetate buffer solution, proportioning, and the addition of bone morphogenetic protein is as shown in table 2.
Then the above-mentioned collagen acetate buffer solution for preparing is respectively mixed with bone morphogenetic protein guanidine hydrochloride-acetate buffer solution, stir at a certain temperature, and leave standstill a period of time.Both mix whipping temp, time of repose, and both mixed proportions are as shown in table 2.
In above-mentioned both mixed liquors, add ethanol subsequently, behind the vibration mix homogeneously, under-50--80 ℃ temperature, preserve certain hour, carry out centrifugation immediately.Storage temperature and time are as shown in table 3 behind amount of alcohol added, the mixing.
With 85% and 100% ethanol precipitate is cleaned respectively at last, clean airing after, add 10% gelatin, under 30 ℃ of temperature, granular sludge is modulated molding.
Bone morphogenetic protein and the compound dosage form of collagen that the embodiment of the invention 2 is produced are put into opposite hole type implantation body, in the implantation alveolar bone, just have after one month a large amount of bone trabecula tooth implant to the hole in form, and merge with alveolar bone, can prevent the loosening of Dental Implant effectively.
The addition of table 1 embodiment collagen and acetate buffer solution, concentration and acid-base value
The addition of the concentration of table 2 embodiment guanidine hydrochloride and acetate buffer solution, proportioning and bone morphogenetic protein
Table 3 embodiment amount of alcohol added, storage temperature and time behind the mixing
Claims (2)
1, the manufacture method of a kind of bone morphogenetic protein and the compound dosage form of collagen particle is characterized in that this method comprises following processing step:
(1) preparation collagen acetate buffer solution
Granulous collagen is dissolved in the 50-60 ℃ of acetate buffer solution, soaked 0.3-1.0 hour, the addition of collagen is 0.1-0.6mg/ml in the acetate buffer solution,
The acid-base value PH of acetate buffer solution is 4.5-5.0, and acetate concentration 15-25mM gets the collagen acetate buffer solution thus;
(2) preparation bone morphogenetic protein guanidine hydrochloride-acetate buffer solution adds bone morphogenetic protein in the mixed liquor of guanidine hydrochloride and acetate buffer solution, and the addition of bone morphogenetic protein is the 0.3-0.7mg/ml of guanidine hydrochloride-acetate buffer solution mixed liquor,
The concentration of guanidine hydrochloride is 5-7M,
The acid-base value PH of acetate buffer solution is 4.5-5.0, and concentration is 12-25mM,
The proportioning of guanidine hydrochloride and acetate buffer solution (ml/ml) is (0.5-1.5): (1.5-2.5);
(3) the above-mentioned collagen acetate buffer solution of preparation is respectively mixed with bone morphogenetic protein guanidine hydrochloride-acetate buffer solution mixed liquor, and under 20-30 ℃ of temperature, stir, left standstill afterwards 0.5-2 hour; Both mixed proportions are 1: 1;
(4) ethanol is added in the above-mentioned mixed liquor, behind the vibration mixing, under-50--80 ℃ temperature, preserved 0.5-1.5 hour, carry out centrifugation immediately,
Alcoholic acid addition (ml) is 1.5-2.5 a times of mixed liquor;
(5) with 85% and 100% ethanol precipitate is washed respectively, with 85% alcohol flushing twice, once with 100% alcohol flushing, the nontoxic airing down in flushing back behind the airing, adds 10% gelatin, under 30 ℃ of temperature, granular sludge is modulated molding, after lyophilization, preserve stand-by.
2, method according to claim 1, the granularity that it is characterized in that collagen are 50-500 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125300A CN1068198C (en) | 1998-12-21 | 1998-12-21 | Production of composite preparation of bone morphogenesis protein and collagen particle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125300A CN1068198C (en) | 1998-12-21 | 1998-12-21 | Production of composite preparation of bone morphogenesis protein and collagen particle |
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| Publication Number | Publication Date |
|---|---|
| CN1230402A CN1230402A (en) | 1999-10-06 |
| CN1068198C true CN1068198C (en) | 2001-07-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN98125300A Expired - Fee Related CN1068198C (en) | 1998-12-21 | 1998-12-21 | Production of composite preparation of bone morphogenesis protein and collagen particle |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100355787C (en) * | 2005-12-26 | 2007-12-19 | 烟台正海生物技术有限公司 | Activated collagen bone repair material and special fusion active bone repair factor thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4394370A (en) * | 1981-09-21 | 1983-07-19 | Jefferies Steven R | Bone graft material for osseous defects and method of making same |
| EP0784985A1 (en) * | 1994-09-30 | 1997-07-23 | Yamanouchi Pharmaceutical Co. Ltd. | Osteoplastic graft |
| WO1997031661A1 (en) * | 1996-02-29 | 1997-09-04 | Lindholm T Sam | An osteogenic device and a method for preparing the device |
-
1998
- 1998-12-21 CN CN98125300A patent/CN1068198C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4394370A (en) * | 1981-09-21 | 1983-07-19 | Jefferies Steven R | Bone graft material for osseous defects and method of making same |
| EP0784985A1 (en) * | 1994-09-30 | 1997-07-23 | Yamanouchi Pharmaceutical Co. Ltd. | Osteoplastic graft |
| WO1997031661A1 (en) * | 1996-02-29 | 1997-09-04 | Lindholm T Sam | An osteogenic device and a method for preparing the device |
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| CN1230402A (en) | 1999-10-06 |
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