CN106818465A - The method and proliferated culture medium of a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm - Google Patents
The method and proliferated culture medium of a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm Download PDFInfo
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- CN106818465A CN106818465A CN201710060992.0A CN201710060992A CN106818465A CN 106818465 A CN106818465 A CN 106818465A CN 201710060992 A CN201710060992 A CN 201710060992A CN 106818465 A CN106818465 A CN 106818465A
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- chinese toon
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- crow chinese
- mutation breeding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The method and proliferated culture medium of a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm, are related to plant breeding.It is carrying material with ripe open country crow Chinese toon seed, space flight is carried out by carrying recoverable spacecraft, after mutagenesis terminates, culture seedling, tissue cultures are carried out from plant axillary bud, gemmule point directly forms the raw seedling of children without callus process, and the new germ plasm of beneficial variation is obtained by cutting tissue-cultured seedling top branch culture plant regeneration seedling, after artificial screening.The composition of Space Mutation Breeding culture open country crow Chinese toon proliferated culture medium is as follows：Modified MS medium；The BA of (0.5~1.5) mg/L 6；(0.1~0.3) mg/L NAA；(25~30) g/L white sugar；(7~9) g/L agar；PH value is 5.8~6.2.The composition of the modified MS medium includes main nutrient elements, micronutrient element and organic reagent.
Description
Technical field
The present invention relates to plant breeding, method more particularly, to a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm and
Proliferated culture medium.
Background technology
Wild crow Chinese toon (Euscaphis japonica (Thunb.) Kanitz) is Staphyleaceae (Staphyleaceae) open country crow
Toona dungarunga.The four seasons are evergreen, bark canescence, tree-like grace；Between spring and summer, flower yellow-white, collection is born in Zhi Ding, Man Shuyin
Flower；9~October of fruit is ripe, cerise Fruit pod cracking, bears fruit to April in next year, for up to 7~8 months, in being gardens
Leaf is seen well, see flower, fruit viewing trees.Wild crow Chinese toon seed, Chinese medicine is referred to as corn eyeball, chicken gizzard, has middle benefit gas qi-regulating, detumescence to stop
Bitterly, treat for stomachache, cold hernia, rush down dysentery, prolapse of the anus, metroptosis, testiclar gall the effects such as；The effects such as its root has removing toxic substances, heat-clearing, dampness removing,
For treating cold headache, dysentery, enteritis etc.；The effects such as it really has expelling wind and clearing away cold, promoting qi circulation and relieving pain, for irregular menstruation, colic,
Stomachache.Timber can be utensil material；Seed oil content 25%~38%, seed oil can soapmaking；Bark can extract tannin extract.Therefore,
Wild crow Chinese toon is a kind of very big seeds of development potentiality.Wild crow Chinese toon seed lasts generally 3 years, and emergence rate from harvesting to sowing
It is low, and excellent variation property can not be kept.The shortcoming of these seeding and seedling raisings, the nursery production and utilization of the wild crow Chinese toon of restriction.
Plant Tissue Breeding, also known as cultured in vitro, is the theory for having totipotency according to plant cell, using plant from
Organ, tissue, cell of body etc. carry out vegetative propagation, with breeding efficiently, growth cycle is short, keep maternal characteristic, can factory
The advantages of changing nursery.It is that a kind of space technology is mutually tied with modern biotechnology and Space Mutation Breeding is also known as Breeding by Space Mutagenesis
Close, beneficial variation, and then the method for screening new germ plasm are produced come inducing plant by the change of space environment.
Chinese patent CN101263789A discloses a kind of method for tissue culture of stem segment of euscaphis konishii, and its selection is sprouted then
Send out sprouting full or be outside shade with the full stem section of giving birth to then of bud, by Fiber differentiation, can sprout birth within 20~25 days
Healthy and strong sprouting long；Again by the amplification cultivation of 20~30 days or so, after root induction culture in 15~20 days or so can seedling,
Transplant, transplanting survival rate is up to more than 90%；Growing fine after surviving, plant shape stalwartness are tall and straight, expand numerous coefficient high.
The content of the invention
It is an object of the invention in order to overcome the above-mentioned deficiency of prior art, there is provided a kind of Space Mutation Breeding culture is wild
The method of crow Chinese toon new germ plasm.
One kind that space mutagenesis open country crow Chinese toon Multiplying culture effect can be effectively improved another object of the present invention is to provide is empty
Between mutation breeding culture open country crow Chinese toon proliferated culture medium.
The method of Space Mutation Breeding culture open country crow Chinese toon new germ plasm is comprised the following steps that：
With ripe open country crow Chinese toon seed as material is carried, space flight is carried out by carrying recoverable spacecraft, mutagenesis terminates
Afterwards, seedling is cultivated, tissue cultures is carried out from plant axillary bud, gemmule point directly forms the raw seedling of children without callus process, and
By cutting tissue-cultured seedling top branch culture plant regeneration seedling, the new germ plasm of beneficial variation is obtained after artificial screening.
The composition of Space Mutation Breeding culture open country crow Chinese toon proliferated culture medium is as follows：
Modified MS medium；
(0.5~1.5) mg/L 6-BA；
(0.1~0.3) mg/L NAA；
(25~30) g/L white sugar；
(7~9) g/L agar；
PH value is 5.8~6.2.
The composition of the modified MS medium includes main nutrient elements, micronutrient element and organic reagent；
The component of the main nutrient elements and its corresponding concentration include：
The component of the micronutrient element and its corresponding concentration include：
The component of the organic reagent and its corresponding concentration include：
The present invention provides a kind of modified MS medium for being more suitable for the crow Chinese toon propagation growth of space mutagenesis open country, changes therein
The composition and content of main nutrient elements, suitably reduce the proportion contained by main nutrient elements in culture medium, effectively overcome
The defect that xylophyta does not tolerate to culture medium salt content, and the nutrition needed for ensure that plant strain growth.Propagation training of the invention
Foster base can increase substantially space mutagenesis open country crow Chinese toon somatic embryo occur frequency and regeneration frequencies, improve breeding efficiency,
It is exaggerated space mutagenesis effect.
The present invention is with the difference of China CN101263789 (the hereinafter referred to as invention)：
1) object of research is different.Of the invention pair as if space mutagenesis open country crow Chinese toon, the invention pair as if knuckle-tooth open country crow
Chinese toon.Space mutagenesis open country crow Chinese toon is that wild crow Chinese toon seed carrying " divine boat ten " airship enters space 15 days, by space intense radiation, height
The particular surroundings mutagenic treatment of vacuum, microgravity and other not clear factors, the mutagenic effect for the generation of wild crow Chinese toon is unclear,
But different from common wild crow Chinese toon, more different from another plant -- euscaphis konishii.
2) culture medium is different.The inducing culture of the invention is：MS+BA(2mg/L)+NAA(0.3mg/L)+Su(20g/L)
+ Ag (7g/L)+Ac (2~2.5g/L) or MS+Zu (2~5mg/L)+IAA (1mg/L)+NAA (0.3mg/L)+Su (39g/L)+Ag
(0.7g/L)；Root media is：1/2MS+IBA (0.5mg/L)+Su (20g/L)+Ag (7g/L), with induction training of the invention
Support base, root media different.
3) condition of culture is different：The condition of culture of the invention is：12~16h/d of light application time, intensity of illumination 1500~
2000 1x, 24 ± 1 DEG C of temperature.Condition of culture of the invention is：Light application time 12h/d, the 1x of intensity of illumination 2000, temperature 25 ±
2℃。
The beneficial effects of the invention are as follows：
1) ease for operation：The present invention is being ensured by selecting improvement MS as the basal medium of space mutagenesis open country crow Chinese toon
Under the supply conditions of calcium constituent, the proportion contained by macronutrient in culture medium is suitably reduced, effectively overcome woody plant
The defect that thing is not tolerated to culture medium salt content, and the nutrition needed for ensure that plant strain growth.
2) high efficiency：Culture medium of the present invention can increase substantially wild crow Chinese toon somatic embryo occur frequency and regeneration plant frequently
Rate, improves breeding efficiency, is exaggerated space mutagenesis effect.
Specific embodiment
The present invention is further illustrated for following examples.
Embodiment 1
A kind of method of Space Mutation Breeding culture open country crow Chinese toon new germ plasm, chooses the wild crow Chinese toon seed of mature and plump, carries
" Divine Land ten " airship enters space 15 days, carries out Space Mutation Breeding, after seeding and seedling raising, obtains annual seedling, choosing
The second side shoot eye is taken, plus liquid detergent is cleaned up, and 10min is rinsed in flowing water, sterilize 30s, sterilized water in 75% ethanol of immersion
Rinse 3 times, sterilize 8min in 0.1% mercuric chloride of immersion, aseptic water washing 5 times drains away the water, 03~0.5cm of excision branch section minor details
Part is impregnated with by medicine, blade is cut off, be seeded in improvement MS+6-BA (1.0mg/L)+NAA (0.2mg/L)+white sugar (25g/L)+
Evoked callus on the culture medium of agar (7g/L), same medium of transferring again is shifted after 30 days, wait plant strain growth to 3~
4cm is high, chooses being inoculated into root media 1/2WPM+NAA (0.3mg/L)+IBA (0.3mg/L) without offspring for robust growth,
After culture 25~30 days, seedling grows root system and normal growth.
Embodiment 2
A kind of method of Space Mutation Breeding culture open country crow Chinese toon new germ plasm, chooses the wild crow Chinese toon seed of mature and plump, carries
" Divine Land ten " airship enters space 15 days, carries out Space Mutation Breeding, after seeding and seedling raising, obtains annual seedling, cuts
The second side shoot eye is taken, flowing water rinses 15min, and sterilize 30s in 75% ethanol of immersion, and aseptic water washing 3 times immerses 0.1% liter
Sterilize 8min in mercury, aseptic water washing 5 times, drains away the water, and excision branch section 03~0.5cm of minor details is impregnated with part by medicine, cuts off
Blade, is seeded in be induced on the culture medium of improvement MS+0.5mg/L 6-BA+0.1mg/L NAA+25g/L white sugar+7g/L agar and heals
Injured tissue, is transferred to the culture medium of improvement MS+1.0mg/L 6-BA+0.2mg/L NAA+25g/L white sugar+8g/L agar after 20 days
Upper acquisition regrowth, screens after 25~30 days to growth of seedling situation and qualitative character, is transferred to root media 1/3WPM+
After IBA (0.3mg/L)+NAA (0.3mg/L) is cultivated 25~30 days, tissue-cultured seedling band bottle moves on to hardening 7 days in horticultural facility, then
Move in the canopy room that sunshade net is set, be transplanted in the cultivation cup containing bactericidal nurishing soil and cultivate 7~10 days, the tissue culture that will be survived
Seedling is placed in outdoor normal sunshine environment lower refining seedling 7 days, is then transplanted to field in, and build sunshade net, it is to avoid just the dusk time-division
Noon direct sunlight.Adequate water is kept after transplanting in 7 days, sunshade net is removed after 30 days, carry out normal field planting management.
Claims (5)
1. the method for a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm, it is characterised in that it is comprised the following steps that：
With ripe open country crow Chinese toon seed as material is carried, space flight is carried out by carrying recoverable spacecraft, after mutagenesis terminates, training
Seedling is supported, tissue cultures are carried out from plant axillary bud, gemmule point directly forms the raw seedling of children without callus process, and by cutting
Tissue-cultured seedling top branch culture plant regeneration seedling is taken, the new germ plasm of beneficial variation is obtained after artificial screening.
2. Space Mutation Breeding culture open country crow Chinese toon proliferated culture medium, it is characterised in that its composition is as follows：
Modified MS medium；
(0.5~1.5) mg/L 6-BA；
(0.1~0.3) mg/L NAA；
(25~30) g/L white sugar；
(7~9) g/L agar；
PH value is 5.8~6.2；
The composition of the modified MS medium includes main nutrient elements, micronutrient element and organic reagent.
3. Space Mutation Breeding culture as claimed in claim 2 open country crow Chinese toon proliferated culture medium, it is characterised in that the Major Nutrient
The component of element and its corresponding concentration include：
4. Space Mutation Breeding culture as claimed in claim 2 open country crow Chinese toon proliferated culture medium, it is characterised in that the micronutrient
The component of element and its corresponding concentration include：
5. Space Mutation Breeding culture as claimed in claim 2 open country crow Chinese toon proliferated culture medium, it is characterised in that the organic reagent
Component and its corresponding concentration include：
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| CN201710060992.0A CN106818465A (en) | 2017-01-25 | 2017-01-25 | The method and proliferated culture medium of a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm |
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| CN201710060992.0A CN106818465A (en) | 2017-01-25 | 2017-01-25 | The method and proliferated culture medium of a kind of Space Mutation Breeding culture open country crow Chinese toon new germ plasm |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632694A (en) * | 2021-09-15 | 2021-11-12 | 中航量子(北京)健康科技有限公司 | Space vegetable cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263789A (en) * | 2008-05-08 | 2008-09-17 | 福建农林大学 | Tissue culture method for stem segment of euscaphis konishii |
| CN101595845A (en) * | 2009-07-21 | 2009-12-09 | 福建农林大学 | Stripped embryo culture of euscaphis konishii and plant regeneration method |
| CN105660365A (en) * | 2016-01-26 | 2016-06-15 | 吉林省蔬菜花卉科学研究院 | Method for breeding new variety of cucumbers with space technology |
-
2017
- 2017-01-25 CN CN201710060992.0A patent/CN106818465A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263789A (en) * | 2008-05-08 | 2008-09-17 | 福建农林大学 | Tissue culture method for stem segment of euscaphis konishii |
| CN101595845A (en) * | 2009-07-21 | 2009-12-09 | 福建农林大学 | Stripped embryo culture of euscaphis konishii and plant regeneration method |
| CN105660365A (en) * | 2016-01-26 | 2016-06-15 | 吉林省蔬菜花卉科学研究院 | Method for breeding new variety of cucumbers with space technology |
Non-Patent Citations (1)
| Title |
|---|
| 张启翔: "《中国观赏园艺研究进展2016》", 31 July 2016 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632694A (en) * | 2021-09-15 | 2021-11-12 | 中航量子(北京)健康科技有限公司 | Space vegetable cultivation method |
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Application publication date: 20170613 |