CN106811439A - Lactobacillus plantarum, Lactobacillus plantarum bacterium powder and its application as pickle starter - Google Patents
Lactobacillus plantarum, Lactobacillus plantarum bacterium powder and its application as pickle starter Download PDFInfo
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- CN106811439A CN106811439A CN201710230913.6A CN201710230913A CN106811439A CN 106811439 A CN106811439 A CN 106811439A CN 201710230913 A CN201710230913 A CN 201710230913A CN 106811439 A CN106811439 A CN 106811439A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Abstract
The present invention relates to a kind of Lactobacillus plantarum, Lactobacillus plantarum bacterium powder and its application as pickle starter, belong to field of food.The biological deposits of the Lactobacillus plantarum are numbered：CGMCC No.13755.Lactobacillus plantarum bacterium powder is by above-mentioned Lactobacillus plantarum and protective agent by weight 1:Obtained through freeze-drying after 50 80 mixing, wherein protective agent includes that weight ratio is 13:2‑3:1.5‑2.5:23 sorbierite, dodecyl sodium sulfate, eight sulfuric acid sucrose and skimmed milk powder.The Lactobacillus plantarum bacterium powder can be used for fermentation pickled vegetable as pickle starter, significantly reduce the content of Nitrite in Pickles, shorten the maturation time of pickle fermentation, improve pickles color and luster and local flavor, while improving the foodsafety of pickles.
Description
Technical field
The present invention relates to field of food, and more particularly to a kind of Lactobacillus plantarum, Lactobacillus plantarum bacterium powder and its conduct bubble
The application of dish leavening.
Background technology
Pickles are a kind of deep cure foods liked by Chinese people, delicious flavour, it is tasty and refreshing can people.From ancient times to the present, no matter
It is that on New Year's Day or other festivals, or in the middle of daily diet, pickles are the delicious food on dining table all the time.Pickles can not only be carried
The appetite of people high, moreover it is possible to the lactic acid bacteria beneficial to human body is produced during fermentation, suppresses the growth of spoilage organisms in enteron aisle,
Promote the digestion of human body.But in the curing process of pickles, the nitrite unfavorable to health can be produced.Nitrous acid
The salt nitrate higher to mostly come from content in vegetables, vegetables absorb the nitrogen in nitrogenous fertilizer or soil, accumulate nontoxic nitre
Hydrochlorate, but in curing process nitrate by some nitrate reductase bacilli shifts into nitrite, so as to produce toxicity.From
The article that Zhang little Hong etc. is delivered for 2006《Harm and preventing and treating of the nitrite in food to human body》, we learn that nitrite is
Strong oxidizer, into blood after combined with hemoglobin, oxyhemoglobin is changed into ferrihemoglobin, so as to lose oxygen carrying
Ability, causes histanoxia.Additionally, nitrite easily synthesizes N- nitrosamine in nature with amine, nitrosamine is a kind of cause
Cancer material.Therefore the nitrite for being produced in the curing process of pickles is unfavorable for the health of people, there is food-safety problem.
So, find a kind of effective method for reducing Nitrite in Pickles content most important.
In national standard《GB2762-2012》Middle clear stipulaties, vegetables and its product, pickless nitrite contain
Amount limitation is 20mg/kg.At present, also it is related to reduce the report of Nitrite in Pickles content.Chinese patent
CN102018154A inoculating lactic acid bacteriums reduce Nitrite in Pickles content, but in pickle fermentation process nitrite
It is of a relatively high；Chinese patent ZL96119451.0 pickled vegetable produced by industrial salt-less fermentation and preparation method thereof is through blanching to vegetable
The nutrition of dish has been destroyed, and is only applicable to the industrial production of fermentation vat.
Therefore, gradually stepped up in people's material life quality, under the background that food-safe problem is increasingly paid attention to, invention
A kind of simple, quick, safety pickle starter, while the method that can effectively reduce Nitrite in Pickles content has important
Meaning.
The content of the invention
The first object of the present invention is to provide a kind of Lactobacillus plantarum, and lactobacillus activity is higher and growth is very fast, energy
Promptly fermenting and producing pickles, while suppressing the growth and breeding of nitrate reductase bacterium.
The second object of the present invention is to provide a kind of Lactobacillus plantarum bacterium powder, the Lactobacillus plantarum bacterium powder viable bacteria content
Height, preserves stabilization, it is adaptable to family and industrial production pickles, while significantly reducing the content of Nitrite in Pickles.
The third object of the present invention is to provide above-mentioned Lactobacillus plantarum bacterium powder to be steeped in Rapid Fermentation as pickle starter
Dish, the application reduced in pickles nitrite.
The present invention is solved its technical problem and is realized using following technical scheme：
The present invention proposes a kind of Lactobacillus plantarum, and its biological deposits numbering is：CGMCC No.13755, the Lactobacillus plantarum
China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) is stored on March 15th, 2017, address is
The Institute of Microorganism, Academia Sinica of Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
The present invention also proposes a kind of Lactobacillus plantarum bacterium powder, and it is by above-mentioned Lactobacillus plantarum and protective agent by weight 1:
It is freeze-dried and obtain after 50-80 mixing.Wherein, protective agent includes that weight ratio is 1-3:2-3:1.5-2.5:The sorb of 2-3
Alcohol, dodecyl sodium sulfate, eight sulfuric acid sucrose and skimmed milk powder.
The present invention also proposes a kind of application of Lactobacillus plantarum bacterium powder as pickle starter.
The beneficial effect of Lactobacillus plantarum bacterium powder is in present pre-ferred embodiments：In Lactobacillus plantarum bacterium powder preparation process
Middle addition protective agent, can change physics, the chemical environment when Lactobacillus plantarum is freezed, and prevent infringement of the water sublimed to cell,
And produce affinity to stablize cell in water and cell by the hydrogen bond and ionic bond contained by protective solute in protective agent
The configuration of composition, mitigates or prevents freezing or rehydration to mycelial infringement, makes strain as keep its original biological living as possible
Property.It is 1-3 to contain weight ratio in protective agent:2-3:1.5-2.5:The sorbierite of 2-3, dodecyl sodium sulfate, eight sulfuric acid sucrose
And skimmed milk powder, not only make protectant cost more cheap, and acted synergistically by said ratio by four kinds of materials, make guarantor
Shield agent is optimal to the protecting effect of Lactobacillus plantarum.Therefore, the Lactobacillus plantarum bacterium powder viable bacteria in present pre-ferred embodiments contains
Amount is high, can be used for quick fermentation production pickles, while significantly reducing the content of Nitrite in Pickles, and preserves stabilization.Its institute
The active component Lactobacillus plantarum for containing has preferably suppress nitrite to act, using the Lactobacillus plantarum bacterium powder as bubble
Dish leavening is used in pickle production, can significantly reduce Nitrite in Pickles content, shortens the maturation time of pickle fermentation, changes
Kind pickles color and luster and local flavor, while improving the foodsafety of pickles.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, below will be attached to what is used needed for embodiment
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, thus be not construed as it is right
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is that the biological deposits information of Lactobacillus plantarum provided in an embodiment of the present invention and survival are proved；
The pictorial diagram of the pickles that Fig. 2 is provided for test example of the present invention.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below will be in the embodiment of the present invention
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional product that can be obtained by commercially available purchase
Product.
Lactobacillus plantarum following to the embodiment of the present invention, Lactobacillus plantarum bacterium powder and its application are specifically described.
It is provided in an embodiment of the present invention for pickle fermentation and reduce nitrite Lactobacillus plantarum bacterium powder, with plant breast
It is after bacillus and protective agent mixing then freeze-dried and obtain.
Wherein, Lactobacillus plantarum is located away from the pickle juice of spontaneous fermentation.Identified, the strain in the embodiment of the present invention is really
Belong to Lactobacillus plantarum category, and its Classification And Nomenclature is Lactobacillus plantarum (Lactobacillus plantarum), and in 2017
March 15 is positioned at No. 3 Chinese microorganism strains of Institute of Microorganism, Academia Sinica of the institute of Chaoyang District Beijing North Star West Road 1
Preservation administration committee common micro-organisms center preservation, deposit number is CGMCC No.13755.Plant used by the present embodiment
The biological deposits information and survival proof of lactobacillus refer to Fig. 1.
The specific screening process of the Lactobacillus plantarum is as follows：In order to improve screening efficiency, screening can for example include primary dcreening operation and
Secondary screening.Wherein primary dcreening operation be typically from substantial amounts of bacterium colony random picking part bacterium colony tested and by detection obtain some compared with
Excellent bacterial strain, leaves out clearly undesirable most of bacterial strain, the bacterial strain that production shape is similar to is tried one's best and is remained, and makes excellent
Strain is unlikely to slip through the net, and the screening type is main based on amount, and accuracy is secondary；Secondary screening is then the more excellent bacterial strain for obtaining primary dcreening operation
Test of many times is carried out again, verifies its potency and mitotic stability, so that therefrom obtain one or two or a few optimal bacterial strain,
The screening type is main based on matter.
Primary dcreening operation for example can be in the pickle juice using MRS agar mediums to spontaneous fermentation lactic acid bacteria screen.Tool
Body ground, gradient dilution is carried out by above-mentioned pickle juice, is then respectively coated in the MRS agar plates containing calcium carbonate, and in
36-54h is cultivated under conditions of 32-38 DEG C.Wherein, the addition of calcium carbonate is preferably the 1-3% of MRS agar medium weight,
Adding the MRS culture mediums after calcium carbonate can make lactic acid bacteria periphery of bacterial colonies form transparent circle (also instant calcium circle), be easy to and remaining bacterium
Plant and make a distinction.Treat that media surface grows bacterium colony, the single bacterium colony with obvious transparent circle in picking culture dish.More preferably, root
According to the growth circle and transparent circle size of strain, the larger single bacterium colony conduct of the ratio between picking 3-5 plants of transparent loop diameter and colony diameter
Primary dcreening operation bacterium.Wherein, MRS agar mediums can for example include that mass ratio is 8-12:3-7:3-5:1.5-2.5:18-22:4-6:
1.5-2.5:0.1-0.3:0.03-0.07:The peptone of 12-18, powdered beef, dusty yeast, Triammonium citrate, glucose, acetic acid
Sodium, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and agar powder, and volume ratio are 7-9:The tween and water of 80-120.By MRS fine jades
The pH of fat culture medium is preferably controlled in the range of 6-6.4, is beneficial to strain growth breeding.Prepare new MRS agar mediums, example
Above-mentioned primary dcreening operation bacterium such as can be inoculated with using streak inoculation mode to be isolated and purified, the primary dcreening operation bacterium for being purified.
Then, the primary dcreening operation bacterium obtained by primary dcreening operation is configured to primary dcreening operation bacterium bacteria suspension respectively, and makes an addition to and contain nitrite
It is blank with the above-mentioned MRS agar mediums containing nitrite without bacteria suspension in MRS agar mediums, determines training
The content of base nitrite is supported, choosing unit cell makes the most bacterial strains of content of nitrite reduction as secondary screening bacterium namely sheet
Lactobacillus plantarum in embodiment, saves backup.After measured, the viable bacteria of the Lactobacillus plantarum that the embodiment of the present invention is filtered out
Quantity is 2.8-3.2 × 109cfu/mL。
Optionally, Lactobacillus plantarum can be by weight 1 with protective agent in the present embodiment:50-80 mixes.Wherein, protect
Shield agent can for example include that weight ratio is 1-3:2-3:1.5-2.5:The sorbierite of 2-3, dodecyl sodium sulfate, eight sulfuric acid sucrose
And skimmed milk powder.It is to prevent strain in freezing dry process because ambient temperature is relatively low to add protectant purpose
And loss of activity, cause viable count to be greatly reduced.Its protection mechanism is：Protective agent can change thing when Lactobacillus plantarum is freezed
Reason, chemical environment, prevent infringement of the water sublimed to cell, and by the hydrogen bond contained by protective solute in protective agent and
Ionic bond produces affinity to stablize the configuration of cell component to water and cell, mitigates or prevent freezing or rehydration to mycelial
Infringement, makes strain keep its original bioactivity as far as possible.
Wherein, sorbierite is used as polyol, with it is more more stable than mannitol the characteristics of, as protective agent into
/ mono-, the mannitol that can be used more in the prior art is lower as protectant scheme cost, and effect is suitable.Dodecyl
Sodium sulfonate is surfactant, can carry out suitably protecting to the protein contained by thalline in freezing dry process.Eight sulfuric acid sugarcanes
Sugar, as glucide, is the non-specific stabilizer of protein, and protein material can be played in each lyophilized stage
Certain protective effect.According to the above ratio by sorbierite, dodecyl sodium sulfate, eight sulfuric acid sucrose and skimmed milk powder collectively as
Plant lactobacillus can be played optimal protecting effect by protectant composition by the synergy between each material.In order that
Protective agent can mix fully and uniform with Lactobacillus plantarum, can before combination, by above-mentioned mannitol, sorbierite, glucose and de-
Fat milk powder carries out heating for dissolving, mixes with Lactobacillus plantarum again after cooling.
The death rate of Lactobacillus plantarum is relatively low in Lactobacillus plantarum bacterium powder after being protected through protective agent, and survival rate is 85-
92%, viable count is 2.5-3 × 109cfu/mL.What deserves to be explained is, after the lactobacillus bacterium powder in the present embodiment can also be by mixing
Lactobacillus plantarum and protective agent be spray-dried or other drying modes and obtain.
Above-mentioned Lactobacillus plantarum bacterium powder can for example be used to produce pickles as pickle starter.Specifically, choose infused
Vegetables, such as cowpea, radish, Chinese cabbage and cabbage etc., clean after remove vegetable surface moisture, be then charged into pickles appearance
In device.Pickled vegetable brine of the configuration containing Lactobacillus plantarum bacterium powder, and is added into container for pickle, also will vegetables be soaked in
In pickled vegetable brine.The ratio of contained edible salt and water can be 1g in the pickled vegetable brine:10-20mL.Because in conventional Kimchi
Nitrite mainly due to vegetables in itself have nitrate higher, in its curing process, nitrate by nitrite also
Opportunistic pathogen changes into poisonous nitrite, so as to produce toxicity.In order that Lactobacillus plantarum can effectively suppress nitrite also
The growth and breeding of opportunistic pathogen, and then the content of pickles nitrite is reduced, preferably by weight 0.07-0.28 in the present embodiment:
100 add into pickled vegetable brine Lactobacillus plantarum bacterium powder.In order to shorten brew time, above-mentioned vegetables can be carried out at cutting
Reason, to increase its contact area with pickled vegetable brine, is more easy to tasty.Additionally, during fermentation, the addition of pickled vegetable brine in container for pickle
Amount is preferably controlled in floods vegetables completely, to avoid brewed effect uneven.
Because Lactobacillus plantarum is anaerobism or facultative anaerobic bacteria, therefore the pickles brew process in the present embodiment is preferable over closed bar
Fermented under part, fermentation temperature for example can be controlled in 15-30 DEG C.In brew process, respectively to contained by the pickles of different brew times
Content of nitrite be measured, as a result show that the content of whole brew process nitrite is no more than 0.253mg/
Kg, well below national standard《GB2714-2003》Specified in content of nitrite (20mg/kg).
What deserves to be explained is, in the present embodiment during fermentation in the proportioning of protectant species and each composition, brew process
Between and temperature be not limited to foregoing description content, related personnel can suitably be adjusted according to actual conditions.
Feature of the invention and performance are described in further detail with reference to embodiments.
Embodiment 1
8 pH of preparation are 6 MRS agar mediums, and each MRS agar medium contains 8g peptones, 3g powdered beefs, 3g
Dusty yeast, 1.5g Triammonium citrates, 18g glucose, 4g sodium acetates, 1.5g dipotassium hydrogen phosphates, 0.1g magnesium sulfate, 0.03g sulfuric acid
Manganese, 12g agar powders and 7mL tweens and 80mL water, add by the 1% of MRS agar medium weight in MRS agar mediums
Calcium carbonate.
The pickle juice of spontaneous fermentation is carried out double dilution of 5 gradients, then by dilution after 5 pickle juices of concentration
It is respectively coated in 5 MRS agar mediums containing calcium carbonate, 54h is cultivated under conditions of 32 DEG C.Treat that media surface is long
Go out bacterium colony, the larger bacterium colony of the ratio between 3 plants of transparent loop diameters of picking and colony diameter is primary dcreening operation bacterium, then by above-mentioned 3 plants of primary dcreening operation bacterium
Streak inoculation respectively is isolated and purified in the remaining 3 MRS agar mediums containing calcium carbonate, obtain after purification just
Sieve bacterium.
4 MRS agar mediums are separately prepared according to the method described above, and 3 culture mediums are separately added into the Asia of equivalent thereto
Nitrate.Then primary dcreening operation bacterium after purification be each configured to the bacteria suspension of same concentrations, and made an addition to above-mentioned contain Asia respectively
In 3 MRS agar mediums of nitrate.It is blank with the MRS agar mediums without bacteria suspension, determines each culture medium
The content of nitrite, choosing unit cell makes the most bacterial strain of content of nitrite reduction as Lactobacillus plantarum.The plant
The number of viable of thing lactobacillus is 2.8 × 109cfu/mL。
By weight it is 1 by Lactobacillus plantarum and protective agent:50 mixing, it is then freeze-dried after obtain Lactobacillus plantarum
Bacterium powder.Wherein, it is 1 that protective agent contains weight ratio:2:1.5:2 sorbierite, dialkyl sulfonates, eight sulfuric acid sucrose and skimmed milk
Powder.The survival rate of Lactobacillus plantarum is 85% in the Lactobacillus plantarum bacterium powder, and viable count is 2.5 × 109cfu/mL。
Embodiment 2
10 pH of preparation are 6.4 MRS agar mediums, and each MRS agar medium contains 12g peptones, 7g beef
Powder, 5g dusty yeasts, 2.5g Triammonium citrates, 22g glucose, 6g sodium acetates, 2.5g dipotassium hydrogen phosphates, 0.3g magnesium sulfate, 0.07g
Manganese sulfate, 18g agar powders and 9mL tweens and 120mL water, by MRS agar medium weight 3% to MRS agar mediums
Middle addition calcium carbonate.
The pickle juice of spontaneous fermentation is carried out double dilution of 5 gradients, then by dilution after 5 pickle juices of concentration
It is respectively coated in 5 MRS agar mediums containing calcium carbonate, 36h is cultivated under conditions of 38 DEG C.Treat that media surface is long
Go out bacterium colony, the larger bacterium colony of the ratio between 5 plants of transparent loop diameters of picking and colony diameter is primary dcreening operation bacterium, then by above-mentioned 5 plants of primary dcreening operation bacterium
Streak inoculation respectively is isolated and purified in the remaining 5 MRS agar mediums containing calcium carbonate, obtain after purification just
Sieve bacterium.
6 MRS agar mediums are separately prepared according to the method described above, and 5 culture mediums are separately added into the Asia of equivalent thereto
Nitrate.Then primary dcreening operation bacterium after purification be each configured to the bacteria suspension of same concentrations, and made an addition to above-mentioned contain Asia respectively
In 5 MRS agar mediums of nitrate.It is blank with the MRS agar mediums without bacteria suspension, determines each culture medium
The content of nitrite, choosing unit cell makes the most bacterial strain of content of nitrite reduction as Lactobacillus plantarum.The plant
The number of viable of thing lactobacillus is 3.2 × 109cfu/mL。
Protective agent is carried out into heating for dissolving, after cooling with Lactobacillus plantarum by weight be 80:1 mixing, then through spraying
Lactobacillus plantarum bacterium powder is obtained after drying.Wherein, it is 3 that protective agent contains weight ratio:3:2.5:3 sorbierite, dialkyl group sulfonic acid
Sodium, eight sulfuric acid sucrose and skimmed milk powder.The survival rate of Lactobacillus plantarum is 92% in the Lactobacillus plantarum bacterium powder, and viable count is 3
×109cfu/mL。
Embodiment 3
12 pH of preparation are 6.2 MRS agar mediums, and each MRS agar medium contains 10g peptones, 5g beef
Powder, 4g dusty yeasts, 2g Triammonium citrates, 20g glucose, 5g sodium acetates, 2g dipotassium hydrogen phosphates, 0.2g magnesium sulfate, 0.05g sulfuric acid
Manganese, 15g agar powders and 8mL tweens and 100mL water, add by the 2% of MRS agar medium weight in MRS agar mediums
Enter calcium carbonate.
The pickle juice of spontaneous fermentation is carried out double dilution of 8 gradients, then by dilution after 8 pickle juices of concentration
It is respectively coated in 8 MRS agar mediums containing calcium carbonate, 36h is cultivated under conditions of 38 DEG C.Treat that media surface is long
Go out bacterium colony, the larger bacterium colony of the ratio between 4 plants of transparent loop diameters of picking and colony diameter is primary dcreening operation bacterium, then by above-mentioned 4 plants of primary dcreening operation bacterium
Streak inoculation respectively is isolated and purified in the remaining 4 MRS agar mediums containing calcium carbonate, obtain after purification just
Sieve bacterium.
5 MRS agar mediums are separately prepared according to the method described above, and 4 culture mediums are separately added into the Asia of equivalent thereto
Nitrate.Then primary dcreening operation bacterium after purification be each configured to the bacteria suspension of same concentrations, and made an addition to above-mentioned contain Asia respectively
In 4 MRS agar mediums of nitrate.It is blank with the MRS agar mediums without bacteria suspension, determines each culture medium
The content of nitrite, choosing unit cell makes the most bacterial strain of content of nitrite reduction as Lactobacillus plantarum.The plant
The number of viable of thing lactobacillus is 3 × 109cfu/mL。
Protective agent is carried out into heating for dissolving, after cooling with Lactobacillus plantarum by weight be 65:1 mixing, then through spraying
Lactobacillus plantarum bacterium powder is obtained after drying.Wherein, it is 2 that protective agent contains weight ratio:2.5:2:2.5 sorbierite, dialkyl group sulphur
Sour sodium, eight sulfuric acid sucrose and skimmed milk powder.The survival rate of Lactobacillus plantarum is 88% in the Lactobacillus plantarum bacterium powder, and viable count is
2.7×109cfu/mL。
Embodiment 4
The cowpea that will be cleaned and remove surface moisture is added into container for pickle.By 1g:10mL prepares edible salt and water
Into pickled vegetable brine, and by weight 0.07:100 add to above-mentioned pickle salt the Lactobacillus plantarum bacterium powder obtained by embodiment 1
In water.Cowpea is flooded completely to it to the pickled vegetable brine containing Lactobacillus plantarum bacterium powder is added in container for pickle.Then in 15
DEG C airtight condition under ferment 10 days.
Embodiment 5
Added into container for pickle after the Chinese cabbage stripping and slicing that will clean and remove surface moisture.By 1g:20mL is by edible salt
Pickled vegetable brine is configured to water, and by weight 0.28:100 is supreme by the Lactobacillus plantarum bacterium powder addition obtained by embodiment 2
In stating pickled vegetable brine.Chinese cabbage is flooded completely to it to the pickled vegetable brine containing Lactobacillus plantarum bacterium powder is added in container for pickle
Not yet.Then in fermentation 10 days under 30 DEG C of airtight condition.
Embodiment 6
Added into container for pickle after the cabbage chopping that will clean and remove surface moisture.By 1g:15mL is by edible salt
Pickled vegetable brine is configured to water, and by weight 0.175:100 is supreme by the Lactobacillus plantarum bacterium powder addition obtained by embodiment 3
In stating pickled vegetable brine.Cabbage is flooded completely to it to the pickled vegetable brine containing Lactobacillus plantarum bacterium powder is added in container for pickle
Not yet.Then in fermentation 10 days under 22.5 DEG C of airtight condition.
Embodiment 7
Added into container for pickle after the radish stripping and slicing that will clean and remove surface moisture.By 1g:18mL is by edible salt and water
Pickled vegetable brine is configured to, and by weight 0.2:100 add to above-mentioned pickles the Lactobacillus plantarum bacterium powder obtained by embodiment 3
In salt solution.Radish is flooded completely to it to the pickled vegetable brine containing Lactobacillus plantarum bacterium powder is added in container for pickle.Then in 25
DEG C airtight condition under ferment 10 days.
Test example
Repeat implement above-described embodiment 4-7, and using application of the Lactobacillus plantarum bacterium powder in pickles in embodiment 4-7 as
Test group 1-4, is respectively provided with the control group 1-4 for being not added with Lactobacillus plantarum bacterium powder.Remaining brewing conditions of above-mentioned control group are equal
It is identical with test group 1-4 respectively.Weighed respectively at the 2nd day in brewed fermentation process, the 4th day, the 6th day, the 8th day and the 10th day
Pickles in equivalent test group and control group, using AAS according to《GB5009.33-2010》In《Food nitrite nitric acid
The measure of salt and nitrate》Standard test Nitrite in Pickles content；In addition, the 1st day in brewed fermentation process,
2 days, the 4th day, the 6th day, the 8th day and the 10th natural gift you can well imagine the pickled vegetable liquid for taking equivalent test group and control group, determined with pH meter and steeped
The pH of dish liquid, its result is as shown in Table 1 and Table 2.
The content of nitrite of table 1 (mg/kg)
| 2nd day | 4th day | 6th day | 8th day | 10th day | |
| Test group 1 | 0.27 | 0.52 | 0.09 | 0.12 | 0.11 |
| Control group 1 | 35.73 | 43.8 | 33.25 | 9.46 | 2.2 |
| Test group 2 | 0.13 | 0.21 | 0.06 | 0.07 | 0.05 |
| Control group 2 | 33.15 | 39.41 | 31.52 | 10.07 | 2.66 |
| Test group 3 | 0.15 | 0.12 | 0.03 | 0.17 | 0.11 |
| Control group 3 | 34.55 | 40.61 | 30.75 | 9.11 | 2.49 |
| Test group 4 | 0.22 | 0.31 | 0.06 | 0.11 | 0.10 |
| Control group 4 | 31.72 | 38.64 | 30.44 | 9.85 | 2.27 |
As can be seen from Table 1, under same fermentation condition, pilot scale in the 2nd day, the 4th day, the 6th day, the 8th day and the 10th day of fermenting
The content of nitrite control group 1-4 corresponding well below its of group 1-4 is tested, while the nitrite in all fermentation process contains
Amount is also well below national standard 20mg/kg, the plant in illustrating by adding the embodiment of the present invention in pickles brew process
Lactobacillus bacterium powder, can significantly reduce the content of nitrite of pickles.
The change of pH value during the pickle fermentation of table 2
As can be seen from Table 2, under same fermentation condition, ferment the 1st day, the 2nd day, the 4th day, the 6th day, the 8th day and the 10th
Test group 1-4 is considerably lower compared with the pH value that pickled vegetable liquid in corresponding control group 1-4 is distinguished with it in it, illustrates to be steeped by pickles
The Lactobacillus plantarum bacterium powder in the embodiment of the present invention is added during system, the pH value of pickled vegetable liquid can be made to be reduced to 3 at second day
Left and right.And the pH variation tendencies by pickled vegetable liquid can be seen that the pH for reaching same sample value, the fermentation time needed for test group will show
The fermentation time needed for being shorter than control group is write, therefore, by the plant in the addition embodiment of the present invention in pickles brew process
Lactobacillus bacterium powder, can substantially shorten the fermenting-ripening time of pickles.
Additionally, refer to Fig. 2, first bottle is obtained by the Lactobacillus plantarum bacterium powder being not added with the embodiment of the present invention in figure
Pickles, second bottle to the 4th bottle for the addition of in the embodiment of the present invention Lactobacillus plantarum bacterium powder obtained by pickles, can be with by figure
Find out, during the Lactobacillus plantarum bacterium powder in adding the embodiment of the present invention in pickles brew process is relatively not added with the embodiment of the present invention
Lactobacillus plantarum bacterium powder obtained by pickles color and luster update vivid pool, and due to reducing the pollution of miscellaneous bacteria, local flavor is also more
Fresh perfume is sharp and clear.
In sum, the Lactobacillus plantarum bacterium powder viable bacteria content of the embodiment of the present invention is high, energy quick fermentation production pickles, together
When significantly reduce the content of Nitrite in Pickles, and preserve stabilization.Active component plant breast bar in Lactobacillus plantarum bacterium powder
Bacterium activity is higher and growth is very fast, so as to promptly inhibit the growth and breeding of nitrate reductase bacterium, significantly reduces pickles
Nitrite.Above-mentioned Lactobacillus plantarum bacterium powder is applied in pickle production as pickle starter, can be significantly reduced
Nitrite in Pickles content, shortens the maturation time of pickle fermentation, improves pickles color and luster and local flavor, while improving the food of pickles
Product security.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of Lactobacillus plantarum, it is characterised in that its biological deposits numbering is：CGMCC No.13755.
2. a kind of Lactobacillus plantarum bacterium powder, as the Lactobacillus plantarum described in claim 1 and protective agent by weight 1:50-80 is mixed
Freeze-dried and obtain after conjunction, the protective agent includes that weight ratio is 1-3:2-3:1.5-2.5:The sorbierite of 2-3, dodecane
Base sodium sulfonate, eight sulfuric acid sucrose and skimmed milk powder.
3. Lactobacillus plantarum bacterium powder according to claim 2, it is characterised in that planted described in the Lactobacillus plantarum bacterium powder
The viable count of thing lactobacillus is 2.5-3 × 109cfu/mL。
4. Lactobacillus plantarum bacterium powder as claimed in claim 2 as pickle starter application.
5. Lactobacillus plantarum bacterium powder according to claim 4 as pickle starter application, it is characterised in that preparation contain
There is the pickled vegetable brine of the Lactobacillus plantarum bacterium powder, vegetables are soaked in the pickled vegetable brine, in fermentation under airtight condition.
6. Lactobacillus plantarum bacterium powder according to claim 5 as pickle starter application, it is characterised in that the bubble
Contain 1g in dish salt solution:The edible salt and water of 10-20mL.
7. Lactobacillus plantarum bacterium powder according to claim 5 as pickle starter application, it is characterised in that the plant
Thing lactobacillus bacterium powder is 0.07-0.28 with the weight ratio of the vegetables:100.
8. Lactobacillus plantarum bacterium powder according to claim 5 as pickle starter application, it is characterised in that fermentation
When, the pickled vegetable brine floods the vegetables completely.
9. Lactobacillus plantarum bacterium powder according to claim 5 as pickle starter application, it is characterised in that fermentation temperature
Spend is 15-30 DEG C.
10. Lactobacillus plantarum bacterium powder according to claim 5 as pickle starter application, it is characterised in that fermentation
During the pickles content of nitrite be no more than 0.253mg/kg.
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