CN106806966A - A kind of autologous fat transplantation equipment for increasing fat cell survival rate - Google Patents
A kind of autologous fat transplantation equipment for increasing fat cell survival rate Download PDFInfo
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- CN106806966A CN106806966A CN201710055207.2A CN201710055207A CN106806966A CN 106806966 A CN106806966 A CN 106806966A CN 201710055207 A CN201710055207 A CN 201710055207A CN 106806966 A CN106806966 A CN 106806966A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/64—Containers with integrated suction means
- A61M1/67—Containers incorporating a piston-type member to create suction, e.g. syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Abstract
The invention discloses a kind of autologous fat transplantation equipment for increasing fat cell survival rate, the step of liposuction and injection being carried out using multiple liposuction chambers and injecting cavity, so as to disperse in human body to take the position of fat and injection fat, prevent from taking fat position and excessively concentrate the problem and injection fat location of caused cutis laxa excessively to concentrate the low problem of caused fat cell survival rate；And multiple liposuction chambers and injecting cavity are arranged on support using sliding block and slide rail, the syringe needle that then can control each liposuction chamber and injecting cavity by stopping means is in different working faces, so as to meet the structure for taking fat or injection site of human body；Also with processing unit fat cell and fat stem cell are isolated from the adipose tissue for taking out, trained altogether after cultivating respectively, induced lipolysis stem cell breaks up in fat cell to capillary, the survival rate of fat cell is further improved, so as to reduce the number of times of operation.
Description
Technical field
The present invention relates to medical instruments field, and in particular to a kind of autologous fat transplantation of increase fat cell survival rate sets
It is standby.
Background technology
Autologous fat transplantation is that patient's own fat is transplanted into soft tissue defects position, so that being reached plentiful modeling by area
The effect of shape.Chinese patent CN103598901A discloses a kind of fat transplantation device, using the structure of integration, by what is taken out
Fat directly carries out fatty implantation again after carrying out filtering purification, and lipolytic activity is unaffected, and it also avoid fat
Pollution；The fat for extracting in addition is purified through screen pack, and the fat implantation human body after purification, compatibility is good.Simple structure, it is practical
Property is strong, it is easy to industrialization.
But it was found that the fatty survival rate after transplanting is relatively low in real work, generally 30%~60%.It is closed
Key reason is fat cell poor to Ischemic Tolerance power, and transplant Zhou Bianqu adipose tissues recover very fast due to blood fortune, survival
Preferably, and central area is more because can not in time obtain sufficient blood supply, fat cell necrosis.So generally requiring in operative region warp
Cross multiple injection and can be only achieved expected effect, the interval time of multiple injection is generally 3-6 month, and on the one hand multiple injection adds
Operative cycle is grown and has been unfavorable for resuming production for operative site, on the other hand also increased the workload for applying patient, also allowed operation
Person will bear the pain of Repeated Operation, and take the position of fat and occur due to comparing concentration and take cutis laxa corrugation etc. after fat
Symptom.
The content of the invention
Have that fat cell survival rate is not high, it is easy to take fat position to solve above-mentioned existing autologous fat transplantation equipment
Produce cutis laxa the problems such as, the invention provides it is a kind of increase fat cell survival rate autologous fat transplantation equipment, specifically
Scheme is as follows：
A kind of autologous fat transplantation equipment for increasing fat cell survival rate, including collection device, processing unit and injection
Device, it is characterised in that：
The collection device includes taking fat device and blood sampling device, and the fat device that takes includes taking fat support and some liposuctions
Chamber, the fat support that takes is provided with some first slide rails, and the liposuction chamber is provided with some first sliding blocks, and the liposuction chamber passes through
First sliding block is arranged on first slide rail, and the liposuction chamber can move on first slide rail；The liposuction
Piston is provided with chamber, the liposuction chamber is divided into working chamber and negative pressure cavity by the piston, and one end of the negative pressure cavity is provided with negative pressure
Pipeline, one end of the working chamber is provided with removable needle, and it is single that the working chamber is provided with first with the junction of the syringe needle
To valve；Grease outlet is offered on the wall of liposuction chamber side, the grease outlet is connected with and removably goes out fat pipeline, the grease outlet
With it is described go out fat pipeline between be provided with the second check valve；The blood sampling device is injector for medical purpose；The processing unit includes carrying
Device and culture apparatus are taken, the extraction element includes cleaning part, centrifugation portion and digestion portion, and the cleaning part is used for acquirement
Fat cell is cleaned, and the centrifugation portion and the digestion portion are used for fractionation of fatty cell and fat stem cell；The culture
Device includes fat stem cell culture systems and co-culture system, is respectively used to the culture of fat stem cell and by fat stem cell
It is inoculated in the culture after fat cell；The injection device includes the injection support and some note relative with fat apparatus structure is taken
Chamber is penetrated, the injection support is provided with some second slide rails, and the injecting cavity is provided with some second sliding blocks, and the injecting cavity leads to
Cross second sliding block to be arranged on second slide rail, the injecting cavity can be moved on second slide rail；The note
Penetrate and offered on the wall of chamber side into fat mouthful, it is described enter fat mouth be connected with and removably enter fat pipeline, it is described enter fat mouthful with it is described enter fat
The 3rd check valve is provided between pipeline.
Further, it is all provided between the liposuction chamber and first slide rail and the injecting cavity and second slide rail
Limited location device, it is ensured that liposuction chamber and injecting cavity can be fixed on positions different on slide rail, so as to ensure liposuction chamber and injection
The syringe needle in chamber may be on different working faces.
Further, the stopping means include be arranged at the caging bolt of the liposuction chamber and described injecting cavity one end with
And some spacing holes opened up on first slide rail and second slide rail, reached by the way that whether caging bolt is stretched into spacing hole
To the purpose whether position of liposuction chamber and injecting cavity limits.
Further, the cleaning part includes being loaded with the cell ware of cleaning fluid, for the adipose tissue of taking-up to be carried out
Cleaning.
Further, the centrifugation portion includes centrifuge tube and centrifuge, for the adipose tissue after cleaning to be centrifuged
So as to obtain the fat cell for going for.
Further, the digestion portion includes being loaded with the test tube of digestive juice, for the adipose tissue after cleaning to be carried out
Digestion, for obtaining fat stem cell.
Further, the fat stem cell culture systems include that culture area is 25cm2Tissue Culture Flask, for will
The fat stem cell for obtaining is cultivated.
Further, the co-culture system includes plug-in type Tissue Culture Dish, and the fat that will cover with Tissue Culture Flask is done
Cell and fat cell are respectively placed in the upper chamber and lower floor of plug-in type Tissue Culture Dish, are then trained altogether.
Further, the nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 15-20mg/mL, described
The concentration of the DMEME culture medium aqueous solution is 5-7.5mg/mL, and the culture additive includes the composition of following parts by weight：1-3
Part baicalein, 5-7.5 parts of Choline Chloride and 2.5-3 parts of sorbierite.
Further, the culture additive also each composition including following parts by weight：1-3 parts of ammonium nitrate, 2-5 parts gather
Methyl methacrylate, 0.5-1 part biotin, 5-10 parts of glutathione, 1-3 parts of sodium lauroyl glutamate.
The beneficial effects of the present invention are：A kind of autologous fat transplantation for increasing fat cell survival rate proposed by the present invention
Equipment, the step of carrying out liposuction and inject using multiple liposuction chambers and injecting cavity, so as to disperse in human body to take fat and injection fat
Position, prevent from taking caused by fat position excessively concentrates the problem and injection fat location of caused cutis laxa excessively to concentrate
The low problem of fat cell survival rate；And multiple liposuction chambers and injecting cavity are arranged on support using sliding block and slide rail, so
The syringe needle that each liposuction chamber and injecting cavity can be controlled by stopping means afterwards is in different working faces, so as to meet human body
The structure for taking fat or injection site；Also with processing unit fat cell and fat are isolated from the adipose tissue for taking out
Fat stem cell, trains altogether after cultivating respectively, and induced lipolysis stem cell breaks up in fat cell to capillary, further improves fat
The survival rate of fat cell, so as to reduce the number of times of operation.
Brief description of the drawings
Fig. 1 are of the invention to take fat apparatus structure schematic diagram,
Fig. 2 are of the invention to take fat cavity configuration enlarged drawing,
Fig. 3 attachment structure schematic diagrams for taking fat chamber and the first slide rail of the invention,
Fig. 4 co-culture system structural representations of the invention,
Fig. 5 co-culture system culturing room structural representations of the invention,
Fig. 6 injection device structural representations of the invention,
Fig. 7 injecting cavity schematic enlarged-scale views of the invention,
The attachment structure schematic diagram of Fig. 8 injecting cavities of the invention and the second slide rail,
Fig. 9 workflow diagrams of the invention.
Figure number and title：1st, fat support is taken, 2, liposuction chamber, the 201, second check valve, 202, go out fat pipeline, 203, pin
Head, the 204, first check valve, 205, working chamber, 206, piston, 207, negative pressure cavity, 208, negative pressure line, the 3, first slide rail, 31,
First sliding block, 4, spacing hole, 5, caging bolt, 6, plug-in type Tissue Culture Dish, 601, upper chamber, 602, lower floor, 7, injecting cavity,
701st, the 3rd check valve, 8, injection support, the 9, second slide rail, the 91, second sliding block.
Specific embodiment
To describe technology contents, structural feature, institute's reached purpose and effect of the present invention in detail, embodiment is hereby enumerated below
And coordinate accompanying drawing to be explained in detail.
Embodiment 1
As shown in figures 1-8, a kind of autologous fat transplantation equipment for increasing fat cell survival rate, including collection device, place
Reason device and injection device, collection device include taking fat device and blood sampling device, and taking fat device includes taking fat support 1 and some suctions
Fat chamber 2, takes fat support 1 and is provided with some first slide rails 3, and liposuction chamber 2 is provided with some first sliding blocks 31, and liposuction chamber 2 is by the
One sliding block 31 is arranged on the first slide rail 3, and liposuction chamber 2 can move on the first slide rail 3；Piston 206 is provided with liposuction chamber 2,
Liposuction chamber 2 is divided into working chamber 205 and negative pressure cavity 207 by piston 206, and one end of negative pressure cavity 207 is provided with negative pressure line 208, work
The one end in chamber 205 is provided with removable needle 203, and working chamber 205 is provided with the first check valve 204 with the junction of syringe needle 203；Inhale
Offer grease outlet on the side wall of fat chamber 2, grease outlet is connected with and removably goes out fat pipeline 202, grease outlet with go out fat pipeline 202 it
Between be provided with the second check valve 201；Blood sampling device is injector for medical purpose；Processing unit includes extraction element and culture apparatus, extracts
Device includes cleaning part, centrifugation portion and digestion portion, and cleaning part is used to be cleaned the fat cell of acquirement, centrifugation portion and digestion
Portion is used for fractionation of fatty cell and fat stem cell；Culture apparatus includes fat stem cell culture systems and co-culture system, point
Not Yong Yu fat stem cell culture and the culture that is inoculated in fat stem cell after fat cell；The injection device includes
The injection support 8 relative with fat apparatus structure is taken and some injecting cavities 7, the injection support 8 are provided with some second slide rails 9,
The injecting cavity 7 is provided with some second sliding blocks 91, and the injecting cavity 7 is arranged at described second and slides by second sliding block 91
On rail 9, the injecting cavity 7 can be moved on second slide rail 9；Offered on the side wall of the injecting cavity 7 into fat mouthful, it is described
Enter fat mouth and be connected with removably to enter fat pipeline, it is described enter fat mouthful and it is described enter fat pipeline between be provided with the 3rd check valve 701.Inhale
Stopping means is equipped between the slide rail 3 of fat chamber 2 and first and the slide rail 9 of injecting cavity 7 and second, it is ensured that liposuction chamber 2 and injecting cavity 7
Positions different on slide rail can be fixed on, so as to ensure that the syringe needle of liposuction chamber 2 and injecting cavity may be at different work and put down
On face.Stopping means includes being arranged at the slide rail 3 of caging bolt 5 and first and the second slide rail of liposuction chamber 2 and the one end of injecting cavity 7
On some spacing holes 4 for opening up, whether the position that liposuction chamber 2 and injecting cavity 7 are reached in spacing hole 4 is stretched into by caging bolt 5
The purpose for whether limiting.
Cleaning part includes cell ware and cleaning fluid, for the adipose tissue of taking-up to be cleaned.Centrifugation portion includes centrifugation
Pipe and centrifuge, for the adipose tissue after cleaning to be centrifuged so as to obtain the fat cell for going for.Wrap in digestion portion
Test tube and digestive juice are included, for the adipose tissue after cleaning to be digested, for obtaining fat stem cell.Fat stem cell is trained
The system of supporting includes that culture area is 25cm2Tissue Culture Flask, the fat stem cell for that will obtain cultivated.Co-culture system
System includes plug-in type Tissue Culture Dish 6, and the fat stem cell and fat cell that will cover with Tissue Culture Flask are respectively placed in plug-in type
In the upper chamber 601 and lower floor 602 of Tissue Culture Dish, then train altogether.
As shown in figure 9, the adipose tissue that liposuction chamber is got is divided into two groups, first group is rinsed with cleaning fluid, removal residual
Haemocyte and fragment of tissue, adipose tissue is put into centrifuge tube, initial volume is recorded, to adding isometric disappearing in tissue
Change liquid, be put into 37 DEG C of gas bath oscillators and digest 30min, the tissue standing 5min that then will digest, after it is layered, with suction
Pipe draws the fat cell positioned at suspension upper strata, after the suspension containing fat cell is centrifuged into 2min with 1000r/min (167g)
Discard lower floor's suspension and obtain fat cell；Second group of adipose tissue is washed down with cleaning fluid, is digested after weighing, when digestion is completed
Afterwards, the indigested adipose tissue in upper strata is discarded, is abandoned after the cell suspension of lower floor is centrifuged into 10min with 1500r/min (375g)
Clearly, obtain being centrifuged the fat stem cell group of bottom of the tube, its is resuspended, and density is adjusted to 1 × 105mL-1, is then seeded in cell
In blake bottle；Afterwards by ready fat stem cell and fat cell be respectively placed in the upper chamber of plug-in type Tissue Culture Dish and under
In layer, common training is carried out using ordinary culture medium；Finally the fat stem cell and fat cell after common training are injected using injecting cavity
Target location.Above-mentioned cleaning fluid is D-hanks solution, and digestive juice is 0.1% NTx isometric with digestion tissue is treated
Enzyme, nutrient solution is the DMEM culture medium aqueous solution of 15mg/mL for the concentration of L-DMEM culture mediums.
Embodiment 2
Embodiment 2 is with the difference of embodiment 1：The nutrient solution is the DMEM trainings containing 15mg/mL culture additives
The base aqueous solution is supported, the concentration of the DMEME culture mediums aqueous solution is 5mg/mL, and the culture additive includes following parts by weight
Composition：1 part of baicalein, 5 parts of Choline Chlorides and 2.5 parts of sorbierites.
Embodiment 3
Embodiment 3 is with the difference of embodiment 1：The nutrient solution is the DMEM trainings containing 20mg/mL culture additives
The base aqueous solution is supported, the concentration of the DMEME culture mediums aqueous solution is 7.5mg/mL, and the culture additive includes following weight portion
Several compositions：3 parts of baicaleins, 7.5 parts of Choline Chlorides and 3 parts of sorbierites.
Embodiment 4
Embodiment 4 is with the difference of embodiment 1：The nutrient solution is the DMEM trainings containing 20mg/mL culture additives
The base aqueous solution is supported, the concentration of the DMEME culture mediums aqueous solution is 5mg/mL, and the culture additive includes following parts by weight
Composition：2 parts of baicaleins, 6 parts of Choline Chlorides, 2.5 parts of sorbierites, 1 part of ammonium nitrate, 2 parts of polymethyl methacrylates, 0.5 part
Biotin, 5 parts of glutathione, 1 part of sodium lauroyl glutamate.
Embodiment 5
The nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 17.5mg/mL, the DMEME culture mediums
The concentration of the aqueous solution is 6mg/mL, and the culture additive includes the composition of following parts by weight：2.5 parts of baicaleins, 7 parts of chlorinations
Choline, 3 parts of sorbierites, 2 parts of ammonium nitrate, 3 parts of polymethyl methacrylates, 0.75 part of biotin, 7.5 parts of glutathione, 2 parts months
Osmanthus acyl sodium glutamate.
Embodiment 6
The nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 18mg/mL, the DMEME culture mediums water
The concentration of solution is 5.5mg/mL, and the culture additive includes the composition of following parts by weight：2.5 parts of baicaleins, 7 parts of chlorinations
Choline, 2.7 portions of sorbierites, 3 portions of ammonium nitrate, 5 portions of polymethyl methacrylates, 1 portion of biotin, 10 portions of glutathione, 3 portions of bays
Acyl sodium glutamate.
Reference examples 1
Difference with embodiment 1 is：The nutrient solution is that the DMEM culture mediums containing 20mg/mL culture additives are water-soluble
Liquid, the concentration of the DMEME culture mediums aqueous solution is 7.5mg/mL, the culture additive include following parts by weight into
Point：7.5 parts of Choline Chlorides and 3 parts of sorbierites.
Reference examples 2
Difference with embodiment 1 is：The nutrient solution is that the DMEM culture mediums containing 20mg/mL culture additives are water-soluble
Liquid, the concentration of the DMEME culture mediums aqueous solution is 7.5mg/mL, the culture additive include following parts by weight into
Point：3 parts of baicaleins and 3 parts of sorbierites.
Reference examples 3
The nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 18mg/mL, the DMEME culture mediums water
The concentration of solution is 5.5mg/mL, and the culture additive includes the composition of following parts by weight：2.5 parts of baicaleins, 7 parts of chlorinations
Choline, 2.7 parts of sorbierites, 3 parts of ammonium nitrate, 1 part of biotin, 10 parts of glutathione, 3 parts of sodium lauroyl glutamates.
Reference examples 4
The nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 18mg/mL, the DMEME culture mediums water
The concentration of solution is 5.5mg/mL, and the culture additive includes the composition of following parts by weight：2.5 parts of baicaleins, 7 parts of chlorinations
Choline, 2.7 parts of sorbierites, 3 parts of ammonium nitrate, 5 parts of polymethyl methacrylates, 1 part of biotin, 3 parts of sodium glutamates
Using the embodiment of the present invention 2,3 and a kind of autologous fat transplantation of increase fat cell survival rate of reference examples 1-2
Equipment carries out fat transfer and culture, at -4 DEG C ± 2 DEG C of temperature, relative humidity be 60% ± 5% under conditions of place 1 month
Afterwards, bacterium situation in detection blake bottle, testing result is shown in Table 1.
The autologous fat transplantation equipment anti-microbial property evaluation result of table 1
Bacterial number considerably less than reference examples 1 in the blake bottle of embodiment 2 and embodiment 3 can be drawn by the data of table 1
There is preferable antibiotic property with reference examples 2, i.e. embodiment 2 and embodiment 3.
Evaluation of the autologous fat transplantation equipment to lipocyte proliferation ability：
Example 4, embodiment 6, reference examples 3, the autologous fat transplantation equipment of reference examples 4, cultivate fat cell, use
Trypan blue classics decoration method is counted to cell, and quantity primary, that the 3rd generation lived fat cell is counted respectively, the results are shown in Table 2.
The fat cell cultured and amplified in vitro effect assessment of table 2
The adipocyte number quantity significantly more than reference examples 4 of embodiment 4 and embodiment 6 can be drawn by the data of table 2,
I.e. embodiment 4 and embodiment 6 are better than reference examples 4 for the culture effect of fat cell.
Evaluation of the autologous fat transplantation equipment to fat cell survival rate：
Example 2, embodiment 4, the autologous fat transplantation equipment of reference examples 2 and 4, cultivate fat cell, are protected at 4 DEG C
Deposit 240 hours, period observes fat cell form when 12h, 24h, 48h, 96h, 240h, and expects blue dyeing meter with platform
Number fat cell survival rate, result of the test is shown in Table 3.
The fat cell survival results of table 3
(wherein：A represents that cell good dispersion degree, uniform in size, form are constant, clear-cut；B represent cell volume become it is big,
Differ in size, soft edge, the abnormal morphology such as oval or irregular occur.)
The fat cell survival rate of embodiment 2 and embodiment 4 apparently higher than the He of reference examples 2 can be drawn by the data of table 3
Reference examples 4, i.e. embodiment 2 and embodiment 4 are better than reference examples 2 and reference examples 4 for the culture effect of fat cell.
In sum, only the preferred embodiments of the invention, does not limit protection scope of the present invention with this, all according to the present invention
Equivalence changes that the scope of the claims and description are made and modification, are all within the scope of patent of the present invention covers.
Claims (10)
1. a kind of autologous fat transplantation equipment for increasing fat cell survival rate, including collection device, processing unit and injection dress
Put, it is characterised in that：
The collection device includes taking fat device and blood sampling device, and the fat device that takes includes taking fat support (1) and some liposuctions
Chamber (2), the fat support (1) that takes is provided with some first slide rails (3), and the liposuction chamber (2) is provided with some first sliding blocks
(31), the liposuction chamber (2) is arranged on first slide rail (3) by first sliding block (31), and the liposuction chamber (2) can
Moved with first slide rail (3)；Piston (206) is provided with the liposuction chamber (2), the liposuction chamber (2) is by the work
Plug (206) is divided into working chamber (205) and negative pressure cavity (207), and one end of the negative pressure cavity (207) is provided with negative pressure line (208), institute
The one end for stating working chamber (205) is provided with removable needle (203), the connection of the working chamber (205) and the syringe needle (203)
Place is provided with the first check valve (204)；Grease outlet is offered on the wall of liposuction chamber (2) side, the grease outlet is connected with detachably
Go out fat pipeline (202), the grease outlet and it is described go out fat pipeline between be provided with the second check valve (201)；The blood sampling device
It is injector for medical purpose；
The processing unit includes extraction element and culture apparatus, and the extraction element includes cleaning part, centrifugation portion and digestion portion,
The cleaning part is used to be cleaned the fat cell of acquirement, and the centrifugation portion and the digestion portion are used for fractionation of fatty cell
And fat stem cell；The culture apparatus includes fat stem cell culture systems and co-culture system, is respectively used to fat dry thin
The culture of born of the same parents and the culture being inoculated in fat stem cell after fat cell；
The injection device includes injection support (8) relative with fat apparatus structure is taken and some injecting cavities (7), the injection branch
Frame (8) is provided with some second slide rails (9), and the injecting cavity (7) is provided with some second sliding blocks (91), the injecting cavity (7)
It is arranged on second slide rail (9) by second sliding block (91), the injecting cavity (7) can be in second slide rail
(9) moved on；Offered on the wall of injecting cavity (7) side into fat mouthful, it is described enter fat mouth be connected with and removably enter fat pipeline, institute
State fat mouthful and it is described enter fat pipeline between be provided with the 3rd check valve (701).
2. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
It is all provided between the liposuction chamber (2) and first slide rail (3) and the injecting cavity (7) and second slide rail (9) limited
Position device.
3. it is according to claim 2 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The stopping means includes being arranged at the caging bolt (5) of the liposuction chamber (2) and the injecting cavity (7) one end and described the
The some spacing holes (4) opened up on one slide rail (3) and second slide rail (9).
4. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The cleaning part includes being loaded with the cell ware of cleaning fluid.
5. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The centrifugation portion includes centrifuge tube and centrifuge.
6. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The digestion portion includes being loaded with the test tube of digestive juice.
7. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The fat stem cell culture systems include that culture area is 25cm2And it is loaded with the Tissue Culture Flask of nutrient solution.
8. it is according to claim 1 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The co-culture system includes plug-in type Tissue Culture Dish (6).
9. it is according to claim 7 it is a kind of increase fat cell survival rate autologous fat transplantation equipment, it is characterised in that：
The nutrient solution is the DMEM culture medium aqueous solution that additive is cultivated containing 15-20mg/mL, the DMEME culture mediums aqueous solution
Concentration be 5-7.5mg/mL, the composition of the culture additive including following parts by weight：1-3 parts of baicalein, 5-7.5 parts of chlorine
Change choline and 2.5-3 parts of sorbierite.
10. a kind of autologous fat transplantation equipment for increasing fat cell survival rate according to claim 9, its feature exists
In：The culture additive also each composition including following parts by weight：1-3 parts of ammonium nitrate, 2-5 parts of poly-methyl methacrylate
Ester, 0.5-1 part biotin, 5-10 parts of glutathione, 1-3 parts of sodium lauroyl glutamate.
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| CN201710055207.2A CN106806966A (en) | 2017-01-24 | 2017-01-24 | A kind of autologous fat transplantation equipment for increasing fat cell survival rate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118944A (en) * | 2017-06-19 | 2017-09-01 | 方育峰 | A kind of device for improving fat cell survival rate in autologous fat DaphniaCarinat a |
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