CN106806886B

CN106806886B - Breath freshener for quickly eliminating halitosis and medical application thereof

Info

Publication number: CN106806886B
Application number: CN201510870376.2A
Authority: CN
Inventors: 陈建国; 吴鹏飞; 王芳; 关鑫磊; 胡壮丽; 龙利红
Original assignee: Huazhong University of Science and Technology
Current assignee: Huazhong University of Science and Technology
Priority date: 2015-12-01
Filing date: 2015-12-01
Publication date: 2021-07-16
Anticipated expiration: 2035-12-01
Also published as: CN106806886A

Abstract

The invention provides a biotechnology breath freshener which eliminates main odor-causing substances in halitosis, namely dimethyl sulfide and the like through enzymatic reaction, thereby quickly eliminating halitosis. The additive has mild and quick action, can be added into products such as mouthwash, toothpaste or chewing gum and the like, can also be added into medicinal preparations or oral medical materials, and has important significance for treating and nursing halitosis.

Description

Breath freshener for quickly eliminating halitosis and medical application thereof

Technical Field

The invention relates to a biotechnology breath freshener, which eliminates main odor-causing substances in halitosis, namely dimethyl sulfide and the like through an enzymatic reaction, can be added into products such as mouthwash, toothpaste or chewing gum and the like, can also be added into medicinal preparations or oral medical materials, and has important significance for treating and nursing halitosis.

Background

Halitosis is an unpleasant gas emitted from the oral cavity or other physiological cavities such as the nose, sinuses, pharynx, etc., and seriously affects social interaction and mental health of people, and WHO has reported halitosis as a disease. The survey shows that the prevalence rate of the Chinese halitosis is about 27.5%. While chronic halitosis is found in 25% of the people worldwide, most adults occasionally have halitosis when the mouth is dry. 80-90% of the bad breath originates from the oral cavity. Gingivitis, periodontitis and oral mucosa diseases in the oral cavity, a large amount of saliva is reduced, and halitosis can be caused by eating certain medicines or irritant foods such as onion, garlic and the like. Scientific data indicate that odorous gas sulfides such as hydrogen sulfide, dimethyl sulfide and the like are the direct causes of halitosis.

Currently, there are two main types of products on the market for breath: firstly, the breath freshener mostly shields the taste of the breath through various odor masking agents with aromatic odor, and the odor masking agents cannot effectively eliminate odor causing halitosis, so that the action time is short; and oral cleaning products, such as mouthwash products, can be used for removing oral bacteria and maintaining oral hygiene. The invention obtains a brand-new high-efficiency breath freshener through a biological enzyme technology and a scientific formula, can quickly remove odor-causing components (dimethyl sulfide and hydrogen sulfide) in breath, eliminate peculiar smell of oral cavity, and can also inhibit oral bacteria for a long time.

Disclosure of Invention

The task of the invention is to provide a biotechnology breath freshener for quickly eliminating halitosis and a medical application thereof.

The technical scheme for realizing the invention is as follows: the biotechnology breath freshener for quickly eliminating the ozostomia provided by the invention comprises 0.1-0.5% of hydrogen peroxide in the proportion; recombinant humanized methionine sulfoxide reductase A0.0001-0.0005%; 0.0001 percent of cysteine; the rest part is mixed with sterile distilled water. The biotechnology breath freshener for preventing and treating halitosis can be directly used, and also can be used for preparing mouthwash, chewing gum, medical products or medical appliances and the like. The biotechnology breath freshener for quickly eliminating the ozostomia can quickly eliminate sulfur-containing organic matters contained in the oral cavity, thereby effectively relieving the ozostomia.

The invention is mainly based on two biochemical reactions discovered in the inventor laboratory, and two main substances causing bad breath odor are respectively eliminated: hydrogen sulfide and dimethyl sulfide.

The biochemical reaction for removing hydrogen sulfide is as follows: under certain physiological conditions and pH environment, hydrogen sulfide generates polythioether under the catalysis of hydrogen peroxide, and then reacts with cysteine to generate cysteine in a sulfhydrylation state.

nH2S+n H2O2→H2(S)n+2n H2O

H2(S)n+Cystein-SH→H2(S)n-1+Cystein-SSH

The biochemical reaction for removing dimethyl sulfide is as follows: under certain physiological conditions and pH environment, dimethyl sulfide can react with hydrogen peroxide with lower concentration rapidly under the catalysis of methionine sulfoxide reductase A to generate odorless dimethyl sulfoxide.

Many mouthwash products have been used to disinfect ulcer surfaces and maintain oral hygiene by adding high concentrations of hydrogen peroxide (1% -5%). However, the high concentration of hydrogen peroxide has great irritation and oxidative damage to oral mucosa, and the single use of hydrogen peroxide has no effect of removing odor-causing components (dimethyl sulfide and hydrogen sulfide) in the oral cavity. The concentration of the hydrogen peroxide used in the invention is less than one tenth of the concentration of the hydrogen peroxide, so that the side effects of the hydrogen peroxide on the oral mucosa, such as irritation, oxidation and the like are effectively avoided. The hydrogen peroxide used by the invention realizes the removal effect of DMS in the breath mainly through the catalytic oxidation effect of methionine sulfoxide reductase A.

Drawings

FIG. 1: the elimination effect of the breath freshener on dimethyl sulfide, a main odor causing substance in halitosis, is detected by using a gas chromatography-mass spectrometry combined technology. As can be seen from fig. 1, the content of dimethyl sulfide (with a peak time of about 1.9 minutes) was significantly reduced after 1 hour of the reaction at 37 c with the addition of the breath freshener.

Detailed Description

The features of the present invention may be further described with reference to the following examples, which should not be construed as in any way limiting the scope of the invention.

Example 1:

a biotech breath freshener comprising: hydrogen peroxide (0.1%); recombinant human methionine sulfoxide reductase A (0.0005%); cysteine (0.0001%); sterile distilled water. The preparation method comprises the following steps: 1 part of medical hydrogen peroxide solution (30%) and 300 parts of sterile distilled water were mixed to prepare a solution, and 0.0005g of recombinant human-derived methionine sulfoxide reductase A and 0.0001g of cysteine were added per 100ml of the solution volume. Filtering under aseptic condition, and packaging into spray bottles. And (5) pressurizing and packaging.

Example 2:

an enzymatic halitosis removing mouthwash. The method comprises the following steps: 1% of peppermint oil, 2% of xylitol, 10% of ethanol, 0.5% of hydrogen peroxide, 1% of calcium hydrophosphate, 86% of distilled water and 0.0005% of methionine sulfoxide reductase A; cysteine (0.0001%). The preparation method comprises the following steps: 5 parts of medical hydrogen peroxide solution (30 percent), 30 parts of ethanol and 300 parts of sterile distilled water are mixed to prepare solution, and 1g of peppermint oil, 2g of xylitol, 1g of calcium hydrophosphate, 0.0005g of recombinant humanized methionine sulfoxide reductase A and 0.0001g of cysteine are added into each 100ml of the solution according to the volume. Filtering under aseptic condition, and bottling.

Example 3:

the scavenging effect of hydrogen peroxide and methionine sulfoxide reductase A on dimethyl sulfide was observed in the proportions written in the patent of the present invention. Dimethyl sulfide in breath is the main odorous substance in healthy people. 50 ml of dimethyl sulfide solution (0.5%) was prepared, and the same volume of hydrogen peroxide (0.1%) and the same volume of methionine sulfoxide reductase A solution (0.0005%) were added and reacted at 37 ℃ for 1 hour. Similarly, 50 ml of dimethyl sulfide solution (0.5%) was prepared, and the same volume of hydrogen peroxide (0.1%) was added as a control, and reacted at 37 ℃ for 1 hour. After the reaction is finished, detecting the content of dimethyl sulfide by gas chromatography-mass spectrometry. It was found that the content of dimethyl sulfide was significantly reduced in the system in which methionine sulfoxide reductase A was added. See fig. 1.

Claims

1. The breath freshener for preventing and treating halitosis is characterized by comprising 0.1 mass percent of hydrogen peroxide, 0.0005 mass percent of recombinant humanized methionine sulfoxide reductase A, 0.0001 mass percent of cysteine and the balance of sterile distilled water.

2. Use of the breath freshener for preventing and treating halitosis according to claim 1 in the preparation of mouth wash and chewing gum.

3. A mouthwash comprising the breath freshener for preventing halitosis according to claim 1.