CN106804704A - A kind of liquid diary product preparation method by adding newborn catalase Shelf-life - Google Patents
A kind of liquid diary product preparation method by adding newborn catalase Shelf-life Download PDFInfo
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- CN106804704A CN106804704A CN201510857001.2A CN201510857001A CN106804704A CN 106804704 A CN106804704 A CN 106804704A CN 201510857001 A CN201510857001 A CN 201510857001A CN 106804704 A CN106804704 A CN 106804704A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C3/00—Preservation of milk or milk preparations
- A23C3/08—Preservation of milk or milk preparations by addition of preservatives
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Abstract
The present invention discloses a kind of liquid diary product preparation method by adding newborn catalase Shelf-life.After methods described includes raw material milk through sterilization processing and cools down, the step of add newborn catalase by aseptic online add-on system, to prepare the liquid diary product containing breast catalase；The addition of the newborn catalase is 1.50g/t-1.8g/t；By electromagnetic radiation sterilization treatment before the newborn catalase addition.The present invention in liquid diary product by adding genuine milk catalase, improve the defense system that liquid diary product itself suppresses microbial reproduction, the liquid diary product for obtaining is set not only to have nutritious effect, also there is unique local flavor and mouthfeel, and with good stability, decline for product storage, logistics and sale middle-end temperature requirement, reduced the rotten risk of product because of each link caused by temperature control is not good, had broad application prospects.
Description
Technical field
The present invention relates to dairy product processing field.More particularly, to one kind by adding newborn hydrogen peroxide
Enzyme suppresses the liquid diary product preparation method of total number of bacteria breeding, Shelf-life to reach.
Background technology
Milk is from mammary gland secretion one kind white out or slightly with yellowish opaque after cow calving
Colloidality liquid, is frequent edible a kind of food in people's daily life.Modern medicine confirms that milk contains
Have various to eugenic beneficial function factor, contained vitamin A is development of fetus and children's life in milk
One of key element long, contributes to pregnant good, placenta normal from infringement, early development.Institute in milk
The vitamin B2 for containing can help the effective replenishing vitamins of pregnancy women, it is to avoid fetal bone deformity.Milk
In contained folic acid, fetal neural tube developmental deformity and brain spina bifida can be prevented, it is to avoid NTD,
Anencephalia youngster and brain Rachischisis are born.Moreover, milk contains rich in protein, fat
The mineral matter such as fat, lactose, vitamin and calcium, iron, sodium, it is considered to be the one kind for being found so far
More satisfactory nutrition equilibrium food.Additionally, milk also has the function of preventing hypertension.Although hypertension
It is a kind of common disease of the elderly.But research shows that this disease can be from fetus in mother's belly
In when start prevention, if in mother of baby be pregnant at her last 3 months, adhering to drinking daily
A certain amount of milk, can just reduce the blood pressure of child, if childhood blood pressure exceed arm's length standard, adult
Often develop into hypertension afterwards, and allow them to have a drink daily milk, so that it may so that systolic pressure is reduced.
Therefore, milk is called the natural enemy of hypertension by people.
At present, the liquid diary product that in the market is sold in addition to Yoghourt and milk beverage, from Technology for Heating Processing form
On, can be largely classified into two kinds of pasteurize and ultra high temperature sterilization.Research shows, with liquid diary product
The lifting of sterilization processing intensity, the destroyed ratio of effective functional component is also improved therewith in product, product
Trophism reduction.But more low intensive process for sterilizing treatment, for the microorganism fatal rate in product and simultaneously
It is undesirable, the shortening for directly resulting in shelf life and the raising for quality risk probability occur.
Accordingly, it is desirable to provide the liquid breast system of the good Shelf-life of a kind of simple and easy to operate and security
Product preparation method.
The content of the invention
It is an object of the present invention to provide a kind of liquid by adding newborn catalase Shelf-life
State dairy product preparation method.Newborn catalase is added in liquid diary product, makes peroxidating in product
Hydrogen, the thiocyanate radical of rhodanate are oxidized, and its compound for producing can suppress the microorganism in milk
Growth, extends shelf life of products；Further, the generation of above-mentioned chemical reaction can also improve liquid diary product
The sense organ local flavor of product itself.
Contain breast according to what method of the present invention production was obtained it is another object of the present invention to provide a kind of
The liquid diary product of catalase.It is prepared into reference to additional newborn catalase using pasteurization methods
To the prolongation effect of shelf life of the liquid diary product become apparent from, while product storage, thing can be reduced
The storage temperature requirement of stream and sales section, and lift the stability of product.
Research shows that newborn catalase is naturally occurring a kind of enzyme in fresh milk, in the whole lactation of ox
Phase, it is 30mg/L that the concentration of newborn catalase is constant always, and newborn catalase and milk are carried itself
Hydrogen peroxide, rhodanate chemically reacted, aoxidize thiocyanate radical, its compound for producing can be with
Suppress the growth of microorganism in milk, and kill some Gram-negative bacterias.
But, in the prior art for newborn hydrogen oxide enzyme in the case of hydrogen peroxide, rhodanate are present
The research of bacteriostasis is only limitted in former breast.At present, also not by newborn catalase added to liquid breast
Correlative study in product to reach the breeding of suppression total number of bacteria, extend shelf life.The applicant grinds
Study carefully discovery, although the content of the newborn catalase in former breast can reach 30mg/L, but, by original
, it is necessary to a series of by pasteurization or UHTS etc. during breast is produced into liquid diary product
Processing procedure, these processing procedures can be damaged to enzyme present in former breast or even produce irreversible shadow
Ring, while also can be to the newborn catalase in former breast, hydrogen peroxide and rhodanate three's system
Balance is damaged, so as to newborn catalase remaining in causing the original after treatment newborn can not be real
Play antibacterial effect.
In order to solve the above problems, the applicant is had found by studying, by the liquid after sterilization processing
Additional appropriate genuine milk catalase promotes additional newborn catalase and dairy in dairy produce
Hydrogen peroxide present in product and rhodanate are chemically reacted, and aoxidize thiocyanate radical, produce suppression
The compound of bacterial reproduction, can reach good fungistatic effect, so as to extend the purpose of shelf life.
To reach above-mentioned first purpose, the present invention uses following technical proposals：
A kind of liquid diary product preparation method by adding newborn catalase Shelf-life, the side
After method includes raw material milk through sterilization processing and cooling, newborn hydrogen peroxide is added by aseptic online add-on system
The step of enzyme, to prepare the liquid diary product containing newborn catalase；The newborn catalase adds
Dosage is 1.5g/t-1.8g/t；By electromagnetic radiation sterilization treatment before the newborn catalase addition.
Preferably, the addition of the newborn catalase is 1.6g/t-1.75g/t.
In this method, the determination of optimal breast catalase addition, is to ensure that it produces fungistatic effect
Key.In this regard, the applicant has carried out substantial amounts of research, the result of study is as shown in Figure 1.This
Applicants have found that, additional newborn catalase 1.5g/t-1.8g/t can peroxidating completely in decomposing milk
Hydrogen and rhodanate.At present, the reaction mechanism of newborn catalase breaks hydrogen peroxide and rhodanate is also
Do not confirmed completely, it is impossible to which the method calculated by numerator equation formula determines optimal newborn hydrogen peroxide
Enzyme addition.The content of hydrogen peroxide and rhodanate in general cow's milk be respectively 30mg/L and 2.90~
34.85mg/L, applicant was respectively by RNA isolation kit and spectrophotometer method to adding newborn catalase
Liquid diary product detected.Research shows that the liquid of addition 1.5g/t-1.8g/t breast catalases is newborn
In product, hydrogen peroxide and rhodanate content are equal<0.1mg/L, basically reaches the effect decomposed completely；
When newborn hydrogen peroxide addition exists<In the range of 1.5g/t, hydrogen peroxide and rhodanate content are in 0.1mg/L
More than, it is not reaching to the effect decomposed completely；When newborn hydrogen peroxide addition is in the range of 1.8-2.2g/t,
Hydrogen peroxide and rhodanate content are also equal<0.1mg/L, but consider the reason such as product cost, be not suitable for by
The numerical value as newborn hydrogen peroxide optimum addition；When newborn hydrogen peroxide addition is in more than 2.2g/t,
Hydrogen peroxide and rhodanate residual content occur again substantially bounce-back, effect compared with<1.5g/t additions are similar to,
Discomposing effect is poor.Therefore Comprehensive Experiment conclusion, add 1.5-1.8g/t newborn catalase enter liquid breast system
Best results in product product, can reach the effect for producing and suppressing product microbial reproduction.
Preferably, the electromagnetic radiation sterilization treatment be by newborn catalase apart from ultraviolet source 25~
In the range of 60cm, wave-length coverage is used to irradiate 20~30min for the ultraviolet of 250~270nm.
Preferably, immobilization treatment is first passed through before the newborn catalase addition, then carries out electromagnetic radiation
Sterilization treatment.
Preferably, the immobilization treatment comprises the following steps：
1) newborn hydrogen peroxide enzyme liquid is well mixed with the acidic alumina of 220~350 mesh, ice bath stirring,
Centrifugation is precipitated thing；
2) sediment is scattered in and suspension is formed in distilled water, suspension is mixed with sodium alginate aqueous solution
Merging is uniformly mixing to obtain mixed liquor；
3) mixed liquor is instilled into CaCl2In solution, mixed liquor drops in CaCl2Shaped in solution and separated out,
Shaped particles are collected, is vacuum dried at room temperature, that is, obtain the newborn catalase of immobilization.
Preferably, the immobilization process step 1) in, it is mixed into 720~780 in every gram of acidic alumina
The newborn hydrogen peroxide enzyme liquid of the 25-40mg/ml of international unit；Step 2) in, every gram of sediment is scattered in
Suspension, the volume that suspension mixes with sodium alginate aqueous solution are formed in 6.05~6.86 milliliters of distilled water
Than being 1：1.5-3, the mass concentration of sodium alginate aqueous solution is 8%~9%；Step 3) in, CaCl2It is molten
The mass concentration of liquid is 25~30%, the load of newborn catalase in the newborn catalase of the immobilization
It is 1-1.5wt% to measure.
Further, in order to ensure the aseptic addition of whole preparation process so that additional newborn hydrogen peroxide
Enzyme will not introduce new bacterium, while consider to ensure the activity of newborn catalase, the invention is characterized in that
Enzyme immobilization technology, embedding treatment is carried out by the lactoperoxidase more sensitive to environment, obtains optimal
Embedding treatment condition, while carrying out electromagnetic radiation sterilization treatment to the newborn catalase of immobilization, add
In adding to liquid diary product so that the newborn catalase after addition immobilization is ensureing itself cleanliness factor
Under the premise of, the hydrogen peroxide for being carried with cow's milk in the product of maximum efficiency, rhodanate carry out chemical anti-
Should, to suppress to remain microbial reproduction in product, reach the purpose of extension shelf life.
The applicant has carried out correlative study to newborn hydrogen peroxide enzyme immobilizatio optimum condition, according to experiment
Result shows, 720~780 international units are mixed into by every gram of aluminum oxide using 220~350 mesh acidic aluminas
Ratio be well mixed with newborn hydrogen peroxide enzyme liquid, and stirring 20 minutes in ice bath are then centrifuged for (1000
Rev/min, 2 DEG C, 15 minutes).Sediment is added 6.05 by every gram of sediment with distilled water~6.86 milliliters
Ratio dispersed with stirring into suspension.Suspension is mixed and stirred for 8%~9% sodium alginate of same volume
Uniformly.Mixed liquor is instilled into 25~30%CaCl2Shaped particles are leached steaming by slow agitation shaping in solution
Distilled water is washed, and vacuum drying is placed in 40 DEG C of storages of drier, gained immobilization at room temperature after 48 hours
Newborn hydrogen peroxide enzyme stability highest.
The applicant also to immobilization after the electromagnetic radiation sterilization treatment condition of newborn catalase grind
Study carefully, result of the test shows, using wave-length coverage be 250~270nm ultraviolet it is effective in 25~60cm
In distance, the newborn catalase surface that irradiation time 20~30 minutes can be effectively to immobilization is present
Microorganism killed.
Applicant also found under study for action, the addition of the newborn catalase of the immobilization, moreover it is possible to improve
The sense organ local flavor of liquid diary product product, bad flavour in removal cow's milk makes in milk good in itself
Milk fragrance release more protrude, preferably cater to consumer taste demand.
Preferably, raw material milk is through sterilization processing and is cooled in the range of 2-4 DEG C, then carries out newborn catalase
Addition.
Preferably, the raw material milk is the fresh milk or recombined milk for meeting China's Fresh Milk acquisition criteria, institute
It is the milk of whole milk, the lowfat milk of partially skimmed or whole degreasings to state fresh milk or recombined milk.
Preferably, the sterilization processing technique of raw material milk include pasteurize treatment, UHTS treatment,
Sterilization treatment or microfiltration of ceramic membrane bacteria removing when low temperature is long.
It is highly preferred that the pasteurize treatment refers to the 10-15s that sterilized at 72-76 DEG C, or at 80-85 DEG C；
The UHTS treatment refers to short period sterilizing of being heated more than 132 DEG C；Sterilized when the low temperature is long
Treatment refers to the 20-35min that sterilized at 63-65 DEG C；The microfiltration of ceramic membrane bacteria removing refers to use
Bacterium and gemma in 0.8-1.4 um pore size ceramic membranes removal raw material milk.
Preferably, the newborn catalase is genuine milk catalase, in extracting from crude milk.
In a certain specific embodiment of the invention, the liquid diary product of the addition lactoperoxidase
Preparation method mainly comprise the following steps：
First, newborn hydrogen peroxide enzyme immobilizatio
1st, every gram is mixed into through 220~350 mesh acidic oxidations with the hydrogen peroxide enzyme liquid of 720~780 international units
In aluminium, and stirred 20 minutes in ice bath, be then centrifuged for (1000 revs/min, 2 DEG C, 15 minutes).
2nd, sediment is added 6.05 in every gram of sediment with distilled water~6.86 milliliters of ratio dispersed with stirring into
Suspension.8%~9% sodium alginate of suspension and same volume is mixed and stirred for uniformly.
3rd, mixed liquor is instilled into 25~30%CaCl2Shaped particles are leached steaming by slow agitation shaping in solution
Distilled water is washed, and to be placed in 40 DEG C of storages of drier standby after being vacuum dried 48 hours at room temperature.
2nd, the newborn catalase electromagnetism sterilizing of immobilization
Newborn catalase after by immobilization is carried out in the case where wave-length coverage is the uviol lamp of 250~270nm
Radio sterilization, newborn catalase after immobilization in the range of 25~60cm of uviol lamp, during irradiation
Between be 20~30 minutes.
3rd, the preparation of the liquid diary product of newborn catalase is added
1st, qualified former milk will be checked by the quiet breast of milk clarifier, the impurity in the former milk of removal.
2nd, according to finished product quality criteria requirements, the former milk after quiet breast is standardized from falling film evaporator.
3rd, by standardization after former milk be warming up to 55-75 DEG C, be de-gassed.
4th, the material after deaerating carries out homogeneous under the conditions of 160-180bar.
5th, the material after homogeneous is by 63 DEG C, 30min, 72-74 DEG C, 15s or 82-55 DEG C, 15s conditions
Under carry out pasteurize, it is desirable to material outlet temperature is in the range of 2-4 DEG C
6th, in the pasteurised milk material after newborn catalase to the sterilization cooling after the aseptic addition treatment of line.
7th, material is cooled into 2-4 DEG C to be stored, obtains the liquid diary product containing lactoperoxidase.
In the present invention, homogeneous is carried out under 55~65 DEG C, 160-180bar to material, can stabilizing it property
More preferably.Feed liquid after homogeneous is under the conditions of 63 DEG C, 30min, 72-74 DEG C, 15s or 82-55 DEG C, 15s
Carry out sterilization, it is ensured that certain sterilizing efficiency.
Specific implementation case of the invention, in the present invention by aseptic online add-on system by immobilization and
In the material cooled down after the lactoperoxidase of sterilization treatment is added to sterilization.
In the present invention, each raw material is commercially available, and each material performance index meets related quality criterion will
Ask.
Packaged form in the present invention to product does not have particular requirement, can use common in the market
The packaged form of low temperature liquid breast.For example, the present invention can be packed using rooftop box, or use PET,
The plastic bottles such as HDPE, BOPP are packed.
Equipment used can be using the known device in this area and related skill in production technology of the invention
Art, no longer repeats specific production equipment and filling process herein.
Present invention also offers the liquid containing newborn catalase prepared according to the method for the present invention
Dairy products.Liquid diary product of the invention, pure flavor has good stability.Specifically, using Pasteur
The liquid diary product product that method for disinfection is prepared with reference to additional newborn catalase, its shelf life
Prolongation effect becomes apparent from.Liquid diary product containing newborn catalase, indices meet national bar
The sterilized breast relevant laws and regulations regulation of family name, can preserve 8-10 days not under low temperature (2-6 degrees Celsius) refrigerated condition
There are the phenomenons such as rotten, fat floating.
Beneficial effects of the present invention are as follows：
In sum, the present invention is improve by adding genuine milk catalase in liquid diary product
Liquid diary product itself suppresses the defense system of microbial reproduction, and contained genuine milk catalase is
Crude milk extracts active component, safe to the human body, have no side effect, and additive is without regulation risk.This
Invention pass through appropriate process meanses, so as to get liquid diary product not only have nutritious effect, also have
There are the local flavor and mouthfeel of uniqueness, and with good stability.Also, specified quantitative is added in the present invention
Genuine milk catalase, moreover it is possible to improve the local flavor of liquid diary product to a certain extent.Simultaneously for
Liquid diary product prepared by the present invention, under product storage, logistics and selling middle-end temperature requirement
Drop, reduces the rotten risk of product because of each link caused by temperature control is not good.It is of the invention to contain day
The liquid diary product of right lactoperoxidase, is the healthy dairy produce for meeting modern's needs, with wide
Application prospect.
Brief description of the drawings
Specific embodiment of the invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 shows adding for additional newborn catalase in the liquid diary product that the inventive method is prepared
The graph of relation of dosage and rhodanate content.
Specific embodiment
In order to illustrate more clearly of the present invention, the present invention is done into one with reference to preferred embodiments and drawings
The explanation of step.Similar part is indicated with identical reference in accompanying drawing.Those skilled in the art
It should be appreciated that following specifically described content is illustrative and be not restrictive, should not be limited with this
Protection scope of the present invention.
In the present invention, in addition to especially indicating, the ratio and content are part by weight and content.
Embodiment 1
Pasteurize liquid diary product preparation method containing genuine milk catalase
First, newborn hydrogen peroxide enzyme immobilizatio
1st, the hydrogen peroxide enzyme liquid of 760 international units is mixed into every gram of 280 mesh acidic aluminas, and in ice
Stirred 20 minutes in bath, be then centrifuged for (1000 revs/min, 2 DEG C, 15 minutes).
2nd, sediment is added into 6.56 milliliters of ratio dispersed with stirring into suspension with distilled water in every gram of sediment
Liquid.8.5% sodium alginate of suspension and same volume is mixed and stirred for uniformly.
3rd, mixed liquor is instilled into 28%CaCl2Shaped particles are leached distillation by slow agitation shaping in solution
Water washing, and to be placed in 40 DEG C of storages of drier standby after being vacuum dried 48 hours at room temperature.Gained is fixed
The load capacity of enzyme is 1.5wt% in the newborn catalase of change.
2nd, the newborn catalase electromagnetism sterilizing of immobilization
Newborn catalase after by immobilization carries out radiation and kills in the case where wave-length coverage is the uviol lamp of 260nm
Bacterium, in the range of uviol lamp 30cm, irradiation time is 25 points to the newborn catalase after immobilization
Clock.
It is prepared by the liquid diary product for the 3rd, adding newborn catalase
1st, qualified former milk will be checked by the quiet breast of milk clarifier, the impurity in the former milk of removal.
2nd, according to finished product quality criteria requirements, the former milk after quiet breast is standardized from falling film evaporator.
3rd, by standardization after former milk be warming up to 65 DEG C, be de-gassed.
4th, the material after deaerating carries out homogeneous under the conditions of 170bar.
5th, the material after homogeneous is killed by carrying out Pasteur under the conditions of 72-74 DEG C, 15s or 82-85 DEG C, 15s
Bacterium, it is desirable to which material outlet temperature is cooled in the range of 2-4 DEG C.
6th, the liquid diary product material after newborn catalase to the sterilization cooling after the aseptic addition treatment of line
In, the addition of the newborn catalase after the immobilization is 120g/t.
7th, material is cooled into 2-4 DEG C to be stored, obtains the liquid diary product containing lactoperoxidase.
Embodiment 2
Sterilize liquid diary product preparation method when low temperature containing genuine milk catalase is long
First, newborn hydrogen peroxide enzyme immobilizatio
1st, the hydrogen peroxide enzyme liquid of 780 international units is mixed into every gram of 350 mesh acidic aluminas, and in ice
Stirred 20 minutes in bath, be then centrifuged for (1000 revs/min, 2 DEG C, 15 minutes).
2nd, sediment is added into 6.86 milliliters of ratio dispersed with stirring into suspension with distilled water in every gram of sediment
Liquid.9% sodium alginate of suspension and same volume is mixed and stirred for uniformly.
3rd, mixed liquor is instilled into 30%CaCl2Shaped particles are leached distillation by slow agitation shaping in solution
Water washing, and to be placed in 40 DEG C of storages of drier standby after being vacuum dried 48 hours at room temperature.Gained is fixed
The load capacity of enzyme is 1.5wt% in the newborn catalase of change.
2nd, the newborn catalase electromagnetism sterilizing of immobilization
Newborn catalase after by immobilization carries out radiation and kills in the case where wave-length coverage is the uviol lamp of 270nm
Bacterium, in the range of uviol lamp 60cm, irradiation time is 30 points to the newborn catalase after immobilization
Clock.
It is prepared by the liquid diary product for the 3rd, adding newborn catalase
1st, qualified former milk will be checked by the quiet breast of milk clarifier, the impurity in the former milk of removal.
2nd, according to finished product quality criteria requirements, the former milk after quiet breast is standardized from falling film evaporator.
3rd, by standardization after former milk be warming up to 75 DEG C, be de-gassed.
4th, the material after deaerating carries out homogeneous under the conditions of 180bar.
5th, the material after homogeneous by carried out under the conditions of 63 DEG C, 30min low temperature it is long when pasteurize, it is desirable to
Material outlet temperature is in the range of 2-4 DEG C.
6th, the liquid diary product material after newborn catalase to the sterilization cooling after the aseptic addition treatment of line
In.The addition of the newborn catalase after the immobilization is 100g/t.
7th, material is cooled into 2-4 DEG C to be stored, obtains the liquid diary product containing lactoperoxidase.
Embodiment 3
The degerming liquid diary product preparation method of microfiltration of ceramic membrane containing genuine milk catalase
First, newborn hydrogen peroxide enzyme immobilizatio
1st, the hydrogen peroxide enzyme liquid of 720 international units is mixed into every gram of 220~350 mesh acidic aluminas, and
Stirred 20 minutes in ice bath, be then centrifuged for (1000 revs/min, 2 DEG C, 15 minutes).
2nd, sediment is added into 6.05 milliliters of ratio dispersed with stirring into suspension with distilled water in every gram of sediment
Liquid.8% sodium alginate of suspension and same volume is mixed and stirred for uniformly.
3rd, mixed liquor is instilled into 25%CaCl2Shaped particles are leached distillation by slow agitation shaping in solution
Water washing, and to be placed in 40 DEG C of storages of drier standby after being vacuum dried 48 hours at room temperature.Gained is fixed
The load capacity of enzyme is 1.3wt% in the newborn catalase of change.
2nd, the newborn catalase electromagnetism sterilizing of immobilization
Newborn catalase after by immobilization carries out radiation and kills in the case where wave-length coverage is the uviol lamp of 250nm
Bacterium, in the range of uviol lamp 25cm, irradiation time is 20 points to the newborn catalase after immobilization
Clock.
It is prepared by the liquid diary product for the 3rd, adding newborn catalase
1st, qualified former milk will be checked by the quiet breast of milk clarifier, the impurity in the former milk of removal.
2nd, according to finished product quality criteria requirements, the former milk after quiet breast is standardized from falling film evaporator.
3rd, by standardization after former milk be warming up to 55 DEG C, be de-gassed.
4th, by standardization after former milk carry out degreasing separation, it is desirable to dilute cream content is less than in defatted milk
0.06%.
5th, defatted milk is carried out into microfiltration of ceramic membrane using the ceramic membrane of 1.0 micron pore sizes under the conditions of 55 DEG C to remove
Bacterium.
6th, the dilute cream isolated sterilizes under the conditions of 125 degrees Celsius, 4 seconds.
7th, the defatted milk on-line mixing after dilute cream and microfiltration of ceramic membrane after sterilizing are degerming, and in 160bar
Under the conditions of carry out homogeneous.
8th, the material after homogeneous under the conditions of 72 DEG C, 15s by carrying out pasteurize, it is desirable to material outlet temperature
Degree is in the range of 2-4 DEG C.
6th, the liquid diary product material after newborn catalase to the sterilization cooling after the aseptic addition treatment of line
In.The addition of the newborn catalase after the immobilization is 118g/t.
7th, material is cooled into 2-4 DEG C to be stored, obtains the liquid diary product containing lactoperoxidase.
Embodiment 4
UHTS liquid diary product preparation method containing genuine milk catalase
First, newborn hydrogen peroxide enzyme immobilizatio
1st, the hydrogen peroxide enzyme liquid of 720 international units is mixed into every gram of 220~350 mesh acidic aluminas, and
Stirred 20 minutes in ice bath, be then centrifuged for (1000 revs/min, 2 DEG C, 15 minutes).
2nd, sediment is added into 6.05 milliliters of ratio dispersed with stirring into suspension with distilled water in every gram of sediment
Liquid.8% sodium alginate of suspension and same volume is mixed and stirred for uniformly.
3rd, mixed liquor is instilled into 25%CaCl2Shaped particles are leached distillation by slow agitation shaping in solution
Water washing, and to be placed in 40 DEG C of storages of drier standby after being vacuum dried 48 hours at room temperature.Gained is fixed
The load capacity of enzyme is 1.4wt% in the newborn catalase of change.
2nd, the newborn catalase electromagnetism sterilizing of immobilization
Newborn catalase after by immobilization carries out radiation and kills in the case where wave-length coverage is the uviol lamp of 250nm
Bacterium, in the range of uviol lamp 25cm, irradiation time is 20 points to the newborn catalase after immobilization
Clock.
It is prepared by the liquid diary product for the 3rd, adding newborn catalase
1st, qualified former milk will be checked by the quiet breast of milk clarifier, the impurity in the former milk of removal.
2nd, according to finished product quality criteria requirements, the former milk after quiet breast is standardized from falling film evaporator.
3rd, standardized material is carried out into homogeneous under the conditions of 180bar.
4th, the material after homogeneous kills bacterium by carrying out superhigh temperature under the conditions of 137 DEG C, 4s, it is desirable to which material goes out
Mouth temperature is in the range of 2-4 DEG C.
6th, the liquid diary product material after newborn catalase to the sterilization cooling after the aseptic addition treatment of line
In.The addition of the newborn catalase after the immobilization is 114g/t.
7th, material is cooled into 2-4 DEG C to be stored, obtains the liquid diary product containing lactoperoxidase.
Comparative example 1
In this comparative example, genuine milk catalase is not added with, but directly carried out by raw material of raw material milk
Processing, other process conditions prepare the liquid diary product of sample 1 as a comparison with embodiment 1.
Comparative example 2
In this comparative example, the newborn catalase of immobilization, but the newborn hydrogen peroxide after immobilization are with the addition of
Enzyme sterilizes without electromagnetic radiation.Other process conditions prepare sample 2 as a comparison with embodiment 1
Liquid diary product.
Comparative example 3
In this comparative example, the newborn catalase without immobilization is with the addition of, the newborn peroxide without immobilization
Electromagnetic radiation sterilizing is have passed through before changing the addition of hydrogen enzyme.Other process conditions prepare conduct with embodiment 1
The liquid diary product of control sample 3.
Liquid diary product sensory evaluation checking containing genuine milk catalase
Producing the product for obtaining to the embodiment of the present invention 1 to embodiment 4 and comparative example 1,2 and 3 carries out mouth
Sense and the Blind Test of local flavor are tested.Primary sensory's inspection project：Structural state, color and luster, mouthfeel, local flavor etc..
Sense organ and flavour mark standard such as table 1.Experiment number totally 30 people is participated in, total score is counted, average mark is calculated；
Average mark is higher, represents effect better；And the overall fancy grade to product provides opinion, it is right to count
The hobby number of each single product；Statistics is recorded in table 1.
The taste and flavor standards of grading of liquid diary product of the table 1 containing natural lactoperoxidase
Evaluation result is recorded in table 2：
The appraisal result of the embodiment of table 2 and comparative example
It is of the invention to add the genuine milk peroxide sterilized by electromagnetic radiation by this sense organ evaluating meter
Change the liquid diary product of hydrogen enzyme compared with control sample, taste and flavor score value is higher.Illustrate this kind of liquid breast
Product is well received, has broad application prospects.Comparative example 1 does not add native catalase, product
There is significant gap compared with embodiment 1-4 in local flavor.
Stability of the liquid diary product containing genuine milk catalase within storage period
In order to detect whether addition genuine milk catalase has an impact to the stability of liquid diary product, send out
The stability of the product in product storage period that a person of good sense obtains to embodiment 1~4 and comparative example 1,2,3 is entered
Go observation, the results are shown in Table 3：
Stability of the product of table 3 within storage period
It can be seen from table 3, the genuine milk peroxide of the immobilization that addition of the invention sterilizes by electromagnetic radiation
Change the liquid diary product of hydrogen enzyme compared with the sample of comparative example, product stability is substantially improved, gained
The product for arriving has preferable stability within storage period, is adapted to commodity production.In comparative example 1, do not add
Enter the liquid diary product of genuine milk catalase, it is right due to the breeding of product microorganism in shelf life
There is obvious negative effect in product quality, stabilising system breakoff phenomenon occurs in shelf life in 7 days.It is right
In ratio 2, the liquid diary product of the genuine milk catalase sterilized without electromagnetic radiation is added,
There is certain effect for suppressing growth of microorganism in product system, but due to adding the genuine milk of immobilization
Catalase sterilizes without electromagnetic radiation, and raw material itself has certain microorganism pollution so that product
Shelf life stability is worse than embodiment, but contrast comparative example 1, still with some superiority.In comparative example 3,
The product of uncured but by radiation sterilization newborn catalase is added, although newborn mistake in comparative example 3
Hydrogen oxide enzyme is without immobilization treatment, but it is sterilized by electromagnetic radiation, and cleanliness factor is higher, estimates and implementation
Example is relatively similar.
Liquid diary product shelf life microbiology turbidity situation containing genuine milk catalase
To (6 DEG C of liquid diary product shelf life in the embodiment of the present invention 1 to embodiment 4 and comparative example 1,2,3
Storage) total number of bacteria is measured, and investigates whether liquid diary product of the invention has extension product shelf
The effect of phase.
Liquid diary product shelf life microbiology turbidity situation of the table 4 containing genuine milk catalase
As can be seen from Table 4, the embodiment 1-3 by the sterilized newborn catalase of electromagnetic radiation is added
Liquid diary product product, microbial reproduction speed is substantially relatively not added with the right of newborn catalase in shelf life
Ratio 1 will weaken, shelf life the 10th day, and product total number of bacteria situation still conforms to pasteurised milk GB
<The laws and regulations requirement of 50000cfu/ml, especially embodiment 4 is due to taking super high temperature sterilizing technology, product
Close to commercial sterilization, therefore total number of bacteria is 0, illustrates that the newborn catalase that with the addition of present invention treatment is effective
The microbial growth inhibited in shelf life.In table 4, comparative example 2 is by the newborn mistake added
, not by electromagnetism sterilization processing, itself microorganism cleanliness factor is weaker, there is pollution sources risk for hydrogen oxide enzyme,
Therefore added in liquid diary product product, though the hydrogen peroxide carried with milk itself, rhodanate are carried out
Chemical reaction, aoxidizes thiocyanate radical, it is certain in the case of inhibit growth of microorganism in milk, but suppress
Effect does not have embodiment 1-3 notable.Though comparative example 3 is sterilized by electromagnetism, itself cleanliness factor is higher,
It is processed without enzyme immobilization, estimates its extension shelf life effect weaker.
Liquid diary product difference storage temperature shelf life microorganism situation containing genuine milk catalase
To liquid diary product different temperatures in the embodiment of the present invention 1 to embodiment 4 and comparative example 1,2,3
Under storage, shelf life (the 7th day, the tenth day) total number of bacteria is measured, and investigates of the invention
Requirement situation of the liquid diary product to shelf life storage temperature.
Shelf life microorganism under liquid diary product difference storage temperature of the table 5 containing genuine milk catalase
Situation
As can be seen from Table 5, influence of the storage temperature to microbial reproduction in shelf life, by adding
The embodiment 1-4 liquid diary products of the sterilized newborn catalase of power-up magnetic radiation do not have comparative example 1,2 and
3 is sensitive.Under 4 DEG C, 6 DEG C, 10 DEG C of storage temperatures, shelf life all can at least reach embodiment 1-4
10 days, and each work-in-process microbial status difference scope is smaller.Although and comparative example 1 is in three temperature
Shelf life is all within 7 days under storage gradient, but product is significantly different in microbial status residing for the 7th day,
Under different storage temperatures, product total number of bacteria is presented significant difference, illustrates addition by addition electromagnetism spoke
The liquid diary product product of newborn catalase of bacterium is shot to death to the shelf life storage temperature more normal Pasteur of requirement
Dairy products relative reduction, in product storage, transports and sells link and can effectively reduce operating cost and product
Quality security risk.Comparative example 2 is poor due to the newborn catalase cleanliness factor of addition, therefore to shelf life
Cause certain harmful effect；Comparative example 3 is processed due to newborn catalase without immobilization, enzymatic activity
It is relatively low, estimate and also directly affect shelf life effect.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and
It is not the restriction to embodiments of the present invention, for those of ordinary skill in the field,
Can also be made other changes in different forms on the basis of described above, here cannot be to all
Implementation method be exhaustive, it is every to belong to the obvious change that technical scheme is extended out
Change or change the row still in protection scope of the present invention.
Claims (10)
1. a kind of liquid diary product preparation method by adding newborn catalase Shelf-life, it is special
Levy and be：After methods described includes raw material milk through sterilization processing and cooling, by aseptic online add-on system
The step of addition breast catalase, to prepare the liquid diary product containing newborn catalase；The breast
The addition of catalase is 1.50g/t-1.8g/t；By electromagnetic radiation before the newborn catalase addition
Sterilization treatment.
2. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：Preferably, the addition of the newborn catalase is
1.6g/t-1.75g/t。
3. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：The electromagnetic radiation sterilization treatment is in distance by newborn catalase
In the range of 25~60cm of ultraviolet source, use wave-length coverage for 250~270nm ultraviolet irradiation 20~
30min。
4. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：Immobilization treatment is first passed through before the newborn catalase addition, then
Carry out electromagnetic radiation sterilization treatment.
5. it is according to claim 4 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：The immobilization treatment comprises the following steps：
1) newborn hydrogen peroxide enzyme liquid is well mixed with the acidic alumina of 220~350 mesh, ice bath stirring,
Centrifugation is precipitated thing；
2) sediment is scattered in and suspension is formed in distilled water, suspension is mixed with sodium alginate aqueous solution
Merging is uniformly mixing to obtain mixed liquor；
3) mixed liquor is instilled into CaCl2In solution, mixed liquor drops in CaCl2Shaped in solution and separated out,
Shaped particles are collected, is vacuum dried at room temperature, that is, obtain the newborn catalase of immobilization.
6. it is according to claim 5 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：The immobilization process step 1) in, mixed in every gram of acidic alumina
Enter the newborn hydrogen peroxide enzyme liquid of the 25-40mg/ml of 720~780 international units；Step 2) in, every gram is sunk
Starch forms suspension, suspension and sodium alginate aqueous solution in being scattered in 6.05~6.86 milliliters of distilled water
The volume ratio of mixing is 1：1.5-3, the mass concentration of sodium alginate aqueous solution is 8%~9%；Step 3)
In, CaCl2The mass concentration of solution is 25~30%, newborn peroxide in the newborn catalase of the immobilization
The load capacity for changing hydrogen enzyme is 1-1.5wt%.
7. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：Raw material milk is through sterilization processing and is cooled in the range of 2-4 DEG C, then carries out
The addition of newborn catalase.
8. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：The sterilization processing technique of raw material milk includes pasteurize treatment, superelevation
Sterilization treatment or microfiltration of ceramic membrane bacteria removing when warm sterilization treatment, low temperature are long.
9. it is according to claim 1 to be made by adding the liquid breast of newborn catalase Shelf-life
Product preparation method, it is characterised in that：The raw material milk is the fresh milk for meeting China's Fresh Milk acquisition criteria
Or recombined milk, the fresh milk or recombined milk are whole milk, the lowfat milk of partially skimmed or whole degreasings
Milk；The newborn catalase is genuine milk catalase, in extracting from crude milk.
10. prepared using the preparation method as described in claim 1-9 is any and contain newborn hydrogen peroxide
The liquid diary product of enzyme.
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Application publication date: 20170609 |