CN106783511A - Esi ion source and its operating method are received based on polarity inversion voltage strategy - Google Patents
Esi ion source and its operating method are received based on polarity inversion voltage strategy Download PDFInfo
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- CN106783511A CN106783511A CN201710111027.1A CN201710111027A CN106783511A CN 106783511 A CN106783511 A CN 106783511A CN 201710111027 A CN201710111027 A CN 201710111027A CN 106783511 A CN106783511 A CN 106783511A
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/10—Ion sources; Ion guns
- H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
- H01J49/165—Electrospray ionisation
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0031—Step by step routines describing the use of the apparatus
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Abstract
The invention discloses a kind of esi ion source of receiving based on polarity inversion voltage strategy, including spraying nozzle needle is received, for loading sample solution；Metal electrode, is inserted into and receives in spraying nozzle needle, with sample solution directly contact；Insulated end cover, closure receive spraying nozzle needle tail end, prevent electric leakage；High voltage power supply, with positive and negative bidirectional output function, is connected with metal electrode, and the electric wire for connecting metal electrode passes through insulated end cover.The present invention is pre-processed first by anti-phase negative high voltage to sample solution, is then produced using normal phase high pressure and is received spraying, and for common nano ESI, with signal intensity higher, signal to noise ratio higher, the tolerance to buffering base status strengthens.In detection process, the expansion of protein molecule foldable structure will not be caused.Device and simple to operate, without additional additive and other preprocessing means.
Description
Technical field
Electrospray mass spectrometry ion gun, more particularly to a kind of spray of receiving based on type reversal voltage strategy are received the present invention relates to one kind
Mist ion gun and its operating method.
Background technology
In the prior art, mass spectrum (mass spectrometry, MS) be one kind by different compounds by mass-to-charge ratio (m/
Z) device for being separated and being detected.Mass spectrum has the ability and chemical specialty of high sensitivity, many materials detection simultaneously, because
And favor is enjoyed in the qualitative and quantitative analysis of compound.In recent years, with life science, especially cell biology is emerging
Rise, mass spectrum is widely used in the detection of trace biomolecular.Many biomolecule have that content is low, sample is difficult to prepare,
And the characteristics of needing to be stored in buffer salt system, this tolerance to mass spectrographic sensitivity and its to buffering base status is proposed
Requirement higher.
Ion gun is the indispensable part of mass spectrum, is also to directly affect Mass Spectrometer Method sensitivity and matrix tolerance
One of critical component.What is proposed in recent years receives esi ion source (nano-electrospray ionization, nano-
ESI) it is a kind of more successful biological sample ion gun.Esi ion source of receiving needs to receive spraying nozzle needle in special drawing
(nano-tip) realized on.Nano-tip is generally hollow glass or quartz ampoule.Through drawing, one end of pipe is formed to
With the 1-10 μm of tip of internal diameter.Before test sample, solution example is injected into nano-tip.Sample size is 5 μ L or so.During detection,
Sample solution is applied in 1-2kV DC high-voltages.Under the driving of high voltage electric field, sample solution forms electricity from nano-tip tips
Spraying, realizes the ionization of biomolecule.
For relative to common electron spray (electrospray ionization, ESI), nano-ESI has relatively low
Flow rate of mobile phase (nL/min ranks) and Ionization Efficiency higher, and there is certain tolerance to buffering base status, more have
Beneficial to the detection of trace biomolecular.Additionally, nano-ESI need not aid in the assistance of gas, device is more simple.These advantages
So that nano-ESI is more widely applied in biochemistry.
In common ESI or nano-ESI, the signal enhancing of positive ion mode is commonly relied on adds additionally in solution
Organic acid (such as 0.1% formic acid or acetic acid).High concentration buffering base status in sample needs to carry out extra pretreatment drop
Low its concentration is thoroughly removed, to prevent from treating the signal generation interference for surveying molecule.
With deepening continuously for application study, people need further to improve the detection sensitivity of nano-ESI and its to slow
Rush the tolerance of base status.
The content of the invention
For above-mentioned shortcoming and defect of the prior art, it is an object of the invention to provide a kind of high detection sensitivity with
And there is height endurability to buffering base status esi ion source and its operating method are received based on polarity inversion voltage strategy.
The purpose of the present invention is achieved through the following technical solutions：
A kind of esi ion source of receiving based on polarity inversion voltage strategy, including
Spraying nozzle needle is received, for loading sample solution；
Metal electrode, is inserted into and receives in spraying nozzle needle, with sample solution directly contact；
Insulated end cover, closure receive spraying nozzle needle tail end, prevent electric leakage；
High voltage power supply, with positive and negative bidirectional output function, is connected with metal electrode, connects the electric wire of metal electrode through absolutely
Acies lid.
Preferably, the positive output voltage range of the high voltage power supply be 0 to+3kV, reverse output voltage range be 0 to-
5kV。
Preferably, the metal electrode is inert electrode.
Preferably, the inert electrode is platinum filament.
A kind of operating method for receiving esi ion source based on polarity inversion voltage strategy, comprises the following steps：
S1, from receive spraying nozzle needle tail end inject sample solution；
S2, by metal electrode from receive spraying nozzle needle tail end be inserted into receive spraying nozzle needle in, until metal electrode is molten with sample
Liquid is touched；
S3, sealed with insulated end cover receive spraying nozzle needle tail end and connect high voltage power supply and metal electrode；
S4, opens high voltage power supply and first exports -2.5kV to 5.0kV voltages to metal electrode, persistently exports 3s to 12s；Then
To metal electrode output+1.5kV to+2.0kV voltages, spraying is received for producing.
Preferably, in step s 4, after high voltage power supply is opened, -3kV voltages, lasting output are first exported to metal electrode
6s；Then+1.75kV voltages are exported to metal electrode.
Compared with prior art, the embodiment of the present invention at least has advantages below：
The present invention is pre-processed first by anti-phase negative high voltage to sample solution, is then produced using normal phase high pressure and is received spray
Mist, for common nano-ESI, the present invention has the advantage that on Detection results：
(1) signal intensity higher：The signal intensity of biomolecule to be measured is strengthened (the 1-2 order of magnitude).
(2) signal to noise ratio higher：The mass spectrogram of gained biomolecule to be measured has signal to noise ratio (1-2 quantity higher
Level).While producing signal enhancing to testing molecule, the signal intensity of noise is restrained effectively, so that testing molecule
Signal to noise ratio significantly increased.
(3) tolerance for buffering base status is strengthened：The buffering base status of higher concentration can be born.For common
Nano-ESI cannot complete the high concentration buffer salt matrix sample (mM grades of concentration) of detection, and the present invention can be examined successfully
Survey.
(4) in detection process, the expansion of protein molecule foldable structure will not be caused.
(5) device and simple to operate, without additional additive and other preprocessing means.In the present invention, without extra
Addition organic acid, it becomes possible to realize significantly increasing for signal.Also, the present invention has to the high concentration buffering base status of mM ranks
Stronger tolerance, without additional pre-treatment means, can be directly realized by the detection to testing molecule.
Brief description of the drawings
Fig. 1 be the present invention based on polarity inversion voltage strategy receive esi ion source structure and voltage apply strategy show
It is intended to；
Fig. 2 a are that the testing results of 10 μM of cytochrome cs are illustrated by commonly receiving the spraying of receiving that esi ion source produces
Figure；
Fig. 2 b are the enlarged drawing of main peak (peak with 8+ charge numbers) in Fig. 2 a；
Fig. 2 c are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to 10 μM by the present invention
The testing result schematic diagram of cytochrome c；
Fig. 2 d are the enlarged drawing of main peak (peak with 8+ charge numbers) in Fig. 2 c；
Fig. 3 a are by commonly receiving testing result schematic diagram of the esi ion source to 100nM cytochrome c samples；
Fig. 3 b are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to 100nM by the present invention
The testing result schematic diagram of cytochrome c；
Fig. 3 c are by commonly receiving testing result schematic diagram of the esi ion source to 10nM cytochrome c samples；
Fig. 3 d are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to 10nM by the present invention
The testing result schematic diagram of cytochrome c；
Fig. 3 e are by commonly receiving testing result schematic diagram of the esi ion source to 1nM cytochrome c samples；
Fig. 3 f be by the present invention based on polarity inversion voltage strategy receive esi ion source produce receive spraying it is thin to 1nM
The testing result schematic diagram of born of the same parents' pigment c；
Fig. 4 a are by commonly receiving testing result schematic diagram of the esi ion source to insulin；
Fig. 4 b are the enlarged drawing of main peak (peak with 5+ charge numbers) in Fig. 4 a；
Fig. 4 c are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to pancreas islet by the present invention
The testing result schematic diagram of element；
Fig. 4 d are the enlarged drawing of main peak (peak with 5+ charge numbers) in Fig. 4 c；
Fig. 5 a are by commonly receiving testing result schematic diagram of the esi ion source to the insulin sample containing NaCl matrix；
Fig. 5 b are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to containing by the present invention
The testing result schematic diagram of the insulin sample of NaCl matrix；
Fig. 6 a are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to concentration by the present invention
It is the cytochrome c ion [M+8H] of the protonation with 8+ electric charges of generation after 10 μM of cytochrome c sample detections8+Extraction
Chromatography of ions figure；
Fig. 6 b are that the receiving for esi ion source generation of receiving based on polarity inversion voltage strategy is sprayed to concentration by the present invention
It is the Na with 8+ electric charges generated after 10 μM of cytochrome c sample detections+Adduct ion [M+5H+3Na]8+Extraction ion color
Spectrogram；
Fig. 6 c are Fig. 6 a and Fig. 6 the b mass spectrograms resulting when first area is on the time；
Fig. 6 d are Fig. 6 a and Fig. 6 the b mass spectrograms resulting when second area is on the time.
Specific embodiment
With reference to embodiment and its accompanying drawing, the invention will be further described, following examples be it is descriptive, no
It is limited, it is impossible to which protection scope of the present invention is limited with this.
A kind of esi ion source of receiving based on polarity inversion voltage strategy, including
Spraying nozzle needle is received, for loading sample solution；The material of spraying nozzle needle received is not limited, generally glass or quartz；Point
End internal diameter is consistent with the common required internal diameter of spraying of receiving, and is 1 to 10 μm.The size of Nano-tip tail ends is not limited.
Metal electrode, is inserted into and receives in spraying nozzle needle, with sample solution directly contact；Electrode material is not limited, but should be had
Chemical inertness, is difficult that chemical reaction causing corrosion and dissolving occur with sample solution.Generally platinum filament.
Insulated end cover, closure receive spraying nozzle needle tail end, prevent electric leakage；
High voltage power supply, with positive and negative bidirectional output function, is connected with metal electrode, connects the electric wire of metal electrode through absolutely
Acies lid.
The positive output voltage range of the high voltage power supply is 0 to+3kV, and reverse output voltage range is 0 to -5kV.
A kind of operating method for receiving esi ion source based on polarity inversion voltage strategy, comprises the following steps：
S1, from receive spraying nozzle needle tail end inject sample solution；
S2, by metal electrode from receive spraying nozzle needle tail end be inserted into receive spraying nozzle needle in, until metal electrode is molten with sample
Liquid is touched；
S3, sealed with insulated end cover receive spraying nozzle needle tail end and connect high voltage power supply and metal electrode；
S4, opens high voltage power supply and first exports -2.5kV to 5.0kV voltages to metal electrode, persistently exports 3s to 12s；Then
To metal electrode output+1.5kV to+2.0kV voltages, spraying is received for producing.More specifically, after high voltage power supply is opened, first
- 3kV voltages are exported to metal electrode, 6s is persistently exported；Then+1.75kV voltages are exported to metal electrode.At the same time, open
Mass detector carries out data acquisition.
When using receive spraying nozzle needle tip align mass spectrometric mass spectrum injection port.
Below by the Detection results of receiving esi ion source of the specific embodiment checking based on polarity inversion voltage strategy.
(1) detection of albuminous cell pigment c
Cytochrome c sterling (derive from horse heart, molecular weight be about 12.4kDa) is dissolved in ultra-pure water and obtains concentration and be
10 μM of testing sample solutions.The solution is detected using common nano-ESI and the method for the present invention respectively, as a result such as Fig. 2
It is shown.
From the testing result (Fig. 2 a) of common nano-ESI as can be seen that gained mass spectrogram to show cytochrome c peculiar
Peak cluster, main peak carry 8+ charge numbers, show cytochrome c molecule be in folded state.To wherein carrying 8+ charge numbers
Main peak is amplified (Fig. 2 b) it can be seen that obvious Na+Ion adducts peak, and adduct peak intensity much stronger than proton
The peak intensity of chemoattractant molecule.Additionally, it is observed that adduction difference Na from figure+The adduct peak of number, including 1 to 6 Na+Ion.
Wherein, with 3 Na+The adduct molecules of ion have highest signal intensity.
In order to quantify to Detection results, hereby to the signal to noise ratio of the protonation cytochrome c molecule with 8+ electric charges
(S/N) calculated, specific calculation is as follows：
S1, the noise signal to mass range in m/z=1480 to 1500 is averaged, and is obtained echo signal peak left end and is made an uproar
The average value of sound.
S2, the noise signal to mass range in m/z=1690 to 1710 is averaged, and is obtained echo signal peak right-hand member and is made an uproar
The average value of sound.
S3, carries out averagely, obtaining the average value of overall noise to left and right two ends noise.
S4, by the signal intensity at echo signal peak divided by the average value of overall noise, obtains final S/N.
In Fig. 2 b, the S/N at the protonation peak with 8+ electric charges obtained by common nano-ESI is 111.
Detection results of the invention are as shown in Figure 2 c.Gained mass spectrogram shows the distinctive peak cluster of cytochrome c, main peak band
There are 8+ charge numbers, show the folding of cytochrome c molecule and be not affected by destruction.Main peak to wherein carrying 8+ charge numbers is put
Greatly after (Fig. 2 d), obvious Na is not observed+The peak of adduct.This shows that the present invention can effectively suppress salt ion adduct
The interference come to detection band, has tolerance higher to base status.From quantitative result it can be seen that (Fig. 2 d), present invention gained
With 8+ electric charges protonation peak S/N be 2180.Compared with common nano-ESI, the S/N obtained by the present invention improves 20
Times.
(2) detection of low concentration cytochrome c
Under low concentration, the present invention is more significantly to the humidification of molecular signal to be measured.We use low concentration
Cytochrome c sample is investigated to this characteristic.Result is as shown in Figure 3.
From the testing result of common nano-ESI (Fig. 3 a, c and e) as can be seen that when the concentration of cytochrome c is 100nM
When, common nano-ESI can reluctantly complete detection, and S/N is 3.And there is obvious Na+The peak of ion adducts occurs.Work as cell
When the concentration of pigment c is reduced to 10nM, the signal of cytochrome c cannot be detected.Further reduce the concentration of cytochrome c
During to 1nM, also without detecting signal.
For the cytochrome c sample of above-mentioned three kinds of concentration, the present invention can favorably accomplish detection (Fig. 3 b, d and f).
When the concentration of cytochrome c is 100nM, the S/N of gained signal is 156, and 53 are enhanced compared with the S/N of common nano-ESI
Times.There is no Na+The peak of adduct occurs.When the concentration of cytochrome c is reduced to 10nM, the S/N of gained signal is 58.No
Na+The peak of adduct occurs.When the concentration of cytochrome c is further reduced to 1nM, the present invention remains to complete detection.Gained
The S/N of signal is 5.Still without Na+The peak of adduct occurs.
(3) detection of proteins insulin
Insulin sterling (deriving from ox pancreas, molecular weight is about 5.6kDa) obtains concentration for 10 μM in being dissolved in ultra-pure water
Testing sample solution.The solution is detected using common nano-ESI and the method for the present invention respectively, as a result such as Fig. 4 institutes
Show.
Be can be seen that (Fig. 4 a) from the testing result of common nano-ESI, gained mass spectrogram shows the distinctive peak of insulin
Cluster, main peak carries 5+ charge numbers.From the enlarged drawing (Fig. 4 b) of main peak it can be seen that obvious Na+Ion adducts peak.Adduction
Different Na+The adduct peak of number can be identified out, including 1 to 9 Na+Ion.Wherein 5 Na of adduction+The spectral peak of ion
With maximum intensity.Due to not observing protonation peak in the main peak peak cluster with 5+ charge numbers, Na is all+Ion adduction
The peak of thing, so cannot be compared with testing result of the invention.This also embodies common nano-ESI and is carrying out insulin
Detection when, the antijamming capability to base status is relatively limited.In view of in the peak cluster with 4+ electric charges, it may be observed that certain
The protonation spectral peak of intensity, thus S/N is calculated from the protonation peak with 4+ electric charges, and carried out with testing result of the invention
Compare.Specific calculation is as follows：
S1, the noise signal to mass range in m/z=1400 to 1420 is averaged, and is obtained echo signal peak left end and is made an uproar
The average value of sound.
S2, the noise signal to mass range in m/z=1560 to 1580 is averaged, and is obtained echo signal peak right-hand member and is made an uproar
The average value of sound.
S3, carries out averagely, obtaining the average value of overall noise to left and right two ends noise.
S4, by the signal intensity at echo signal peak divided by the average value of overall noise, obtains final S/N.With 4+ electricity
The S/N at the protonation peak of lotus is 6.
The present invention effectively improves the testing result to insulin.From testing result it can be seen that (Fig. 4 c), gained matter
Spectrogram shows the distinctive peak cluster of insulin, and main peak carries 5+ charge numbers.(Fig. 4 d) is almost observed not in the enlarged drawing of main peak
To Na+Ion adducts peak.This explanation present invention has stronger volume tolerance to base status.Protonation peak with 4+ electric charges
S/N be 2252.With the testing result of common nano-ESI Comparatively speaking, S/N enhances 375 times.
(4) to the investigation of base status tolerance
Further to investigate tolerance of the present invention to base status, respectively using common nano-ESI and the present invention to containing
The insulin sample of NaCl is detected, and testing result is contrasted.First, NaCl solid dissolvings are obtained in ultra-pure water
It is the NaCl matrix solutions of 1mM to concentration.Afterwards, insulin solid dissolving is obtained into concentration for 10 μM in NaCl matrix solutions
Insulin sample solution, for detecting.
(Fig. 5 a) is it can be seen that obvious NaCl cluster ions from the testing result of common nano-ESI.These clusters from
The appearance of son, causes serious ion depression effect so that the signal of insulin is greatly inhibited.Although the letter of insulin
Number can reluctantly be recognized from figure, but signal intensity is weaker, and have serious Na+Adduction peak occurs.
Detection results of the invention then improve significantly.(Fig. 5 b) is it is observed that pancreas from testing result of the invention
The island distinctive peak cluster of element, main peak carries 5+ charge numbers.There is no the appearance of NaCl cluster ions, this explanation was detected of the invention
Cheng Zhong, the ion depression effect that high concentration NaCl is brought is effectively canceled.This also demonstrates the present invention to mM ranks
Base status has stronger tolerance.
(5) mechanism is investigated
To further appreciate that characteristic of the invention, we have carried out deep spy to signal enhancing of the invention and desalination mechanism
Beg for.Mechanism is investigated using cytochrome c as detection object.It is 10 μM that cytochrome c solid dissolving obtains concentration in ultra-pure water
Sample solution, is directly used in detection.After the completion of detection, the cytochrome c ion [M+ of the protonation with 8+ electric charges is investigated respectively
8H]8+EIC (Extracted ion chromatograms extract chromatography of ions figure) and the Na with 8+ electric charges+Adduct ion
[M+5H+3Na]8+EIC (Fig. 6).
It should be noted that the vertical line left side in the coordinate diagram of Fig. 6 a and Fig. 6 b is first area, vertical line the right is second
Region.
In detection process of the invention, solution first pass around the -3.0kV of 6s negative high voltage treatment (time opens from 0min
Begin).Then, voltage is brought to+1.75kV and receives spraying producing.From now on, [M+8H]8+The signal of ion strengthens rapidly,
Until reaching peak value (Fig. 6 a).Afterwards, [M+8H] 8+The signal of ion gradually weakens.
Na with 8+ electric charges+Adduct ion [M+5H+3Na]8+EIC then have different rules.The ions representative
Na in solution+Behavior.Be brought to+1.75kV when voltage with produce receive spraying after, [M+5H+3Na]8+The signal of ion is more long
More slow growth is maintained in time, until after reaching red area in time, [M+5H+3Na]8+The signal of ion is met
More rapidly growth is carried out.In figure a [M+8H]8+The EIC of ion is compared to as can be seen that the peak value of two kinds of ions is appeared in not
When same.Protonated ion [M+8H]8+Peak value occur when earlier, and Na+ adducts [M+5H+3Na]8+Letter
Number then being in reduced levels at the beginning, entering on the time after second area just gradually strengthens.
From the above it can be seen that inherent mechanism of the invention.Because the testing molecule in solution and matrix salt ion exist
There is different electromigration speed in solution, thus the distance migrated under the driving of the negative high voltage electric field of 6s times also differs
Sample.Matrix salt ion with migration rate higher has migrated larger distance (to the direction away from nano-tip tips), and
Testing molecule has then migrated less distance.After normal electron spray is triggered using positive high voltage, testing molecule just elder generation and matrix
Salt ion produces stronger signal.This just realizes the removal to matrix salt ion, enhances the present invention to sample mesostroma ion
Tolerance.Simultaneously as matrix salt ion is separated with testing molecule so that testing molecule is subject to during ionization
Ion suppress corresponding and reduce, so as to also enhance the Ionization Efficiency of testing molecule, and then enhance the detection of testing molecule
Signal.
The mass spectrogram (Fig. 6 c) that first time period is collected from Fig. 6 is as can be seen that testing molecule has stronger letter
Number intensity, spectral peak has signal to noise ratio higher.The adduct peak of base status ion is not observed in figure.And in the second time
The mass spectrogram (Fig. 6 d) that section is collected is it can be seen that the signal of testing molecule receives obvious interference.There is obvious salt in figure
The peak of ion Na+ ion adducts, and the overall signal of cytochrome c reduces, and spectral peak has relatively low signal to noise ratio.Fig. 6 c
Further proved in the above-mentioned investigation to EIC with two significant differences of mass spectrogram of Fig. 6 d it is concluded that.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in,
Should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Enclose and be defined.
Claims (6)
1. a kind of esi ion source of receiving based on polarity inversion voltage strategy, it is characterised in that including
Spraying nozzle needle is received, for loading sample solution；
Metal electrode, is inserted into and receives in spraying nozzle needle, with sample solution directly contact；
Insulated end cover, closure receive spraying nozzle needle tail end, prevent electric leakage；
High voltage power supply, with positive and negative bidirectional output function, is connected with metal electrode, and the electric wire for connecting metal electrode passes through insulating end
Lid.
2. the esi ion source of receiving based on polarity inversion voltage strategy according to claim 1, it is characterised in that the height
The positive output voltage range of voltage source is 0 to+3kV, and reverse output voltage range is 0 to -5kV.
3. the esi ion source of receiving based on polarity inversion voltage strategy according to claim 1 and 2, it is characterised in that institute
Metal electrode is stated for inert electrode.
4. the esi ion source of receiving based on polarity inversion voltage strategy according to claim 3, it is characterised in that described lazy
Property electrode be platinum filament.
5. a kind of operating method for receiving esi ion source based on polarity inversion voltage strategy, it is characterised in that including following step
Suddenly：
S1, from receive spraying nozzle needle tail end inject sample solution；
S2, by metal electrode from receive spraying nozzle needle tail end be inserted into receive spraying nozzle needle in, until metal electrode connects with sample solution
Contact；
S3, sealed with insulated end cover receive spraying nozzle needle tail end and connect high voltage power supply and metal electrode；
S4, opens high voltage power supply and first exports -2.5kV to 5.0kV voltages to metal electrode, persistently exports 3s to 12s；Then to gold
+ 1.5kV is to+2.0kV voltages for category electrode output, and spraying is received for producing.
6. the operating method for receiving esi ion source based on polarity inversion voltage strategy according to claim 5, its feature
It is in step s 4, after high voltage power supply is opened, -3kV voltages first to be exported to metal electrode, persistently exports 6s；Then to gold
Category electrode output+1.75kV voltages.
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| CN108666201A (en) * | 2017-12-31 | 2018-10-16 | 宁波华仪宁创智能科技有限公司 | Incude Nanoliter electrospray ion source and its working method |
| WO2019129276A1 (en) * | 2017-12-31 | 2019-07-04 | 宁波华仪宁创智能科技有限公司 | Induction nanoliter electrospray ion source and method of operation thereof |
| CN109243965A (en) * | 2018-08-02 | 2019-01-18 | 中国计量科学研究院 | The protein highly charged ion production method of esi ion source is received based on polarity reversion |
| WO2021212920A1 (en) * | 2020-12-08 | 2021-10-28 | 广东省科学院测试分析研究所(中国广州分析测试中心) | Nanoliter spray-fticr-ms analysis method and device for organic matter dissolved in environmental solid sample |
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