CN106772981A - The stereoscopic microscopic imaging device of polarization type dynamic realtime and method - Google Patents
The stereoscopic microscopic imaging device of polarization type dynamic realtime and method Download PDFInfo
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- CN106772981A CN106772981A CN201510811945.6A CN201510811945A CN106772981A CN 106772981 A CN106772981 A CN 106772981A CN 201510811945 A CN201510811945 A CN 201510811945A CN 106772981 A CN106772981 A CN 106772981A
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- illumination light
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
- G02B21/20—Binocular arrangements
- G02B21/22—Stereoscopic arrangements
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/368—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements details of associated display arrangements, e.g. mounting of LCD monitor
Abstract
The invention discloses a kind of stereoscopic microscopic imaging device of polarization type dynamic realtime and method.The device includes the LED array, sample stage, microcobjective, tube lens, the video camera that set gradually from bottom to up, also including polarization type 3D glasses, computer and polarization type 3D displays;Wherein LED array is centrally located on the central axis of microcobjective, tube lens;LED array and video camera are connected with computer.Method is:Sample is placed in sample stage, computer controls LED array is alternately produced two circular illumination light, collected by microcobjective through sample stage, and carry out being incident to tube lens after zooming into picture, computer drives video camera is sampled to the sample image through tube lens, and input polarization formula 3D displays, observer carries out dynamic realtime stereomicroscopy viewing to sample image by wearing polarization type 3D glasses.Apparatus of the present invention are simple, color effects are good, and depth of focus and stereoscopic angle can be adjusted flexibly, and realize the dynamic realtime viewing to sample.
Description
Technical field
The invention belongs to optical microphotograph stereoscopic imaging technology field, particularly a kind of stereoscopic micro-imaging of polarization type dynamic realtime
Device and method.
Background introduction
Human eye has the effect of stereoscopic vision, can distinguish front and rear, the distance of object, and this is because human perception is naturally deep
Degree is because images of left and right eyes viewing real world has slight difference to cause.Stereoscopic vision derives from " parallax ", people's
Eyes have the distance of 4-6cm, and during viewing object, left eye sees that the composition of object left surface is more, and right eye sees object
The composition of right flank is more, so actually we have seen that image during object in two eyes is differentiated, this is poor
It is not referred to as " parallax ".Brain has the ability for understanding parallax, and the two discrepant images are after brain synthesis
The depth hierarchical relationship of object can just be distinguished, so as to produce stereoscopic vision, it is seen that be the image for having the depth of field, this is just
It is the principle of human-eye stereoscopic vision.In general, the parallax value of image is bigger, and third dimension is stronger, that is, showing same thing
During body, two camera simulation human eye distances are bigger, and third dimension is stronger.But distance can not infinitely increase, because when apart from nothing
During limit increase, the condition for obtaining stereoscopic visual effect is no longer met, can not be provided with certain depth information for eyes
Parallax, so as to stereoscopic vision can not be formed.Conventional stereo microscope by a shared primary object lens to image objects after,
The middle objective lens that can be adjusted by two groups of multiplying powers are separated, and are then imaged through the eyepiece group of 12-15 ° of over-angle, such
Words just can respectively provide angle different images for right and left eyes, seen by person to be exactly by after the integrated treatment of brain
One width has the 3 D visual image of the depth of field.
Traditional stereoscope advantage is apparent:It is easy to use, it is easy to operate.Cannot but the problem that it is present is also
Make up:(1) depth of focus and stereoscopic angle are nonadjustable, and due to human eye interpupillary distance individual difference, the stereoscopic angle of human eye is different,
The undesirable of personal viewing effect can so be caused;(2) binary channels light path not only makes instrument heaviness but also cost can also be increased;
(3) visual field is small, and image quality is poor, it is not possible to which dynamic realtime watches the details of object.
The content of the invention
It is an object of the invention to provide a kind of device it is simple, color effects are good, depth of focus and stereoscopic angle can be adjusted flexibly
The stereoscopic microscopic imaging device of polarization type dynamic realtime and method, realize the dynamic realtime viewing to sample, lift observer's body
Degree of testing.
The technical solution for realizing the object of the invention is:A kind of stereoscopic microscopic imaging device of polarization type dynamic realtime, including
P4 series LEDs array, sample stage, microcobjective, tube lens, video camera, polarization type 3D displays,
Computer and polarization type 3D glasses;Wherein P4 series LEDs array, sample stage, microcobjective, tube lens,
Video camera sets gradually from bottom to up, and P4 series LED arrays are centrally located at the center of microcobjective, tube lens
On axis;The control end of P4 series LEDs array and video camera is connected with computer, and the output end of video camera is accessed partially
Formula of shaking 3D displays;
Sample to be imaged is placed in sample stage, computer sends trigger signal, makes to P4 series LEDs array
P4 series LED arrays are alternately produced two circular illumination light, and illumination light is collected through sample stage by microcobjective,
Be amplified for the illumination light of collection tube lens be incident to after imaging by microcobjective, and computer sends to actuated camera and touches
Signalling is sampled with actuated camera to the sample image through tube lens, and video camera is defeated by the sample image for gathering
Enter polarization type 3D displays to be shown, observer watches polarization type 3D displays i.e. by wearing polarization type 3D glasses
The stereomicroscopy structure of sample image can in real time be watched.
A kind of stereoscopic micro imaging method of polarization type dynamic realtime based on described device, step is as follows:
Step 1, sample stage is placed in by sample to be imaged, and computer sends triggering letter to P4 series LEDs array
Number, P4 series LED arrays is alternately produced two circular illumination light;
Step 2, step 1 produce illumination light collected by microcobjective through sample stage, microcobjective by collection
Illumination light is incident to tube lens after being amplified imaging;
Step 3, computer sends trigger signal with actuated camera to the sample drawing through tube lens to actuated camera
As being sampled, obtain corresponding respectively to two microobject images of circular illumination light;
Step 4, video camera makees two microobject image input polarization formula 3D displays that step 3 is photographed respectively
For left-and right-eye images are shown;
Step 5, observer by wear polarization type 3D glasses watch polarization type 3D displays by watch sample in real time
The stereomicroscopy structure of image.
Compared with prior art, its remarkable advantage is the present invention:(1) use of P4 series LEDs array, instead of
The microscopical double light path of conventional stereo, simplifies system design, not only makes imaging system easy but also effective reduces cost;
(2) it is used as lighting source by using LED array, smaller by external interference with system light source in itself, observation
The restricted reduction of condition, can be watched under dark background;(3) by controlling the circle on P4 series LED arrays
The radius r of shape lighting source and the center of circle are realized to the flexible adjustable of depth of focus and stereoscopic angle respectively apart from d;(4) by video camera
The image for collecting is passed on polarization type 3D displays, clear to the dynamic realtime of sample stereochemical structure such that it is able to realize
Viewing, lifts observer's Experience Degree.
Brief description of the drawings
Fig. 1 is the structural representation of the stereoscopic microscopic imaging device of polarization type dynamic realtime of the present invention.
Fig. 2 is the white circle illumination light schematic diagram of P4 series LEDs array generation in the present invention, and (a) figure is initialization
When the white circle illumination light schematic diagram that produces of P4 series LEDs arrays be intended to, (b) figure be a certain moment P4 serial
The white circle illumination light schematic diagram that LED array is produced, (c) figure is the white of subsequent time P4 series LEDs array generation
Color circular illumination light schematic diagram.
Fig. 3 is the principle schematic of the stereoscopic angle of P4 series LED antenna array controls and depth of focus in the present invention.
Fig. 4 is the sample drawing that shot by camera is arrived in the embodiment of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawings and specific embodiment is described in further detail to the present invention.
With reference to Fig. 1, the stereoscopic microscopic imaging device of polarization type dynamic realtime of the present invention, including P4 series LEDs array 1,
Sample stage 2, microcobjective 3, tube lens 4, video camera 5, polarization type 3D displays 6, the and of computer 7
Polarization type 3D glasses 8;Wherein P4 series LEDs array 1, sample stage 2, microcobjective 3, tube lens 4,
Video camera 5 sets gradually from bottom to up, and P4 series LEDs array 1 is centrally located at microcobjective 3, tube lens
On 4 central axis;The control end of P4 series LEDs array 1 and video camera 5 is connected with computer 7, video camera 5
Output end access polarization type 3D displays 6;
Sample to be imaged is placed in sample stage 2, computer 7 sends trigger signal to P4 series LEDs array 1,
P4 series LEDs array 1 is set to be alternately produced two circular illumination light, illumination light is through sample stage 2 by microcobjective
3 collect, and microcobjective 3 is incident to tube lens 4 after the illumination light of collection is amplified into imaging, and computer 7 is to drive
Dynamic video camera 5 is sent trigger signal and is sampled with 5 pairs of sample images through tube lens 4 of actuated camera, is imaged
The sample image input polarization formula 3D displays 6 of collection are shown that observer is by wearing polarization type 3D by machine 5
The viewing polarization type 3D of glasses 8 displays 6 can in real time watch the stereomicroscopy structure of sample image.
Preferably, the P4 series LEDs array 1 provides red, green, blueness, cyan, pink colour, yellow, white
Seven kinds of illumination lights of color of light, the present apparatus uses random color illumination light, and two circular illumination light source colours are identical i.e.
Can;The unit board size of P4 series LED arrays is 128mm*128mm, number of pixels is 32*32, pel spacing
4mm, each pixel can be lighted individually.
Preferably, distance of the P4 series LEDs array 1 apart from the upper surface of sample stage 2 is 75~85mm.
Further, the computer 7 sends trigger signal to P4 series LEDs array 1, makes P4 series LEDs battle array
Row 1 are alternately produced two radiuses for r, center of circle spacing are d, color identical circular illumination light source, and wherein r, d is picture
Vegetarian refreshments number.
Preferably, the CCD camera lenses of the video camera 5 are located on the back focal plane of tube lens 4.
Further, the computer 7 sends to the trigger signal that video camera 5 sends with to P4 series LEDs array 1
Trigger signal keep synchronous.
Polarization type dynamic realtime stereoscopic micro imaging method of the present invention based on claim 1 described device, step is as follows:
Step 1, sample stage 2 is placed in by sample to be imaged, and computer 7 sends to P4 series LEDs array 1
Trigger signal, makes P4 series LEDs array 1 be alternately produced two circular illumination light;
Step 2, the illumination light that step 1 is produced is collected through sample stage 2 by microcobjective 3, and microcobjective 3 will
The illumination light of collection is incident to tube lens 4 after being amplified imaging;
Step 3, computer 7 sends trigger signal and passes through tube lens 4 for 5 pairs with actuated camera to actuated camera 5
Sample image sampled, obtain corresponding respectively to two microobject images of circular illumination light;
Step 4, two 6 points of microobject image input polarization formula 3D displays that video camera 5 photographs step 3
Not shown as left-and right-eye images;
Step 5, observer watches in real time by watching polarization type 3D displays 6 by wearing polarization type 3D glasses 8
The stereomicroscopy structure of sample image.
Further, P4 series LEDs array 1 is alternately produced two circular illumination light described in step 1, two circles
The radius of illumination light is r, center of circle spacing is d, color is identical, and wherein r, d are the number of pixel.
Further, the trigger signal that computer 7 described in step 3 is sent to video camera 5 and step 1 Computer 7 to
The trigger signal that P4 series LEDs array 1 sends keeps synchronous.
Embodiment 1
With reference to Fig. 1, the stereoscopic microscopic imaging device of polarization type dynamic realtime of the present invention, including P4 series LEDs array 1,
Sample stage 2, microcobjective 3, tube lens 4, video camera 5, polarization type 3D displays 6, the and of computer 7
Polarization type 3D glasses 8;Wherein P4 series LEDs array 1, sample stage 2, microcobjective 3, tube lens 4,
Video camera 5 sets gradually from bottom to up, and P4 series LEDs array 1 is centrally located at microcobjective 3, tube lens
On 4 central axis;The control end of P4 series LEDs array 1 and video camera 5 is connected with computer 7, video camera 5
Output end access polarization type 3D displays 6;
Sample to be imaged is placed in sample stage 2, computer 7 sends trigger signal to P4 series LEDs array 1,
P4 series LEDs array 1 is set to be alternately produced two circular illumination light, color is any, as long as ensureing two circular illumination light
Color is identical, as shown in Fig. 2 the white circle illumination light that P4 series LEDs array is produced when (a) is initialization
Schematic diagram is intended to, and (b) is the white circle illumination light schematic diagram that a certain moment P4 series LEDs array is produced, (c)
For the white circle illumination light schematic diagram that subsequent time P4 series LEDs array is produced.Illumination light passes through sample stage 2
Collected by microcobjective 3, microcobjective 3 is incident to tube lens 4 after the illumination light of collection is amplified into imaging, counted
Calculation machine 7 is sent trigger signal and is carried out with 5 pairs of sample images through tube lens 4 of actuated camera to actuated camera 5
The sample image input polarization formula 3D displays 6 of collection are shown sampling, video camera 5 observer is by wearing
The viewing polarization type 3D of polarization type 3D glasses 8 displays 6 can in real time watch the stereomicroscopy structure of sample image.
Preferably, the P4 series LEDs array 1 provides red, green, blueness, cyan, pink colour, yellow, white
Seven kinds of illumination lights of color of light, the present apparatus uses random color illumination light, as long as ensureing two circular illumination light source face
Color is identical;The unit board size of P4 series LEDs array 1 is 128mm*128mm, number of pixels is 32*32,
Pel spacing 4mm, each pixel can be lighted individually.
Preferably, distance of the P4 series LEDs array 1 apart from the upper surface of sample stage 2 is 75~85mm.
Further, the computer 7 sends trigger signal to P4 series LEDs array 1, makes P4 series LEDs battle array
Row 1 are alternately produced two radiuses for r, center of circle spacing are d, color identical circular illumination light source, and wherein r, d is picture
Vegetarian refreshments number.As shown in figure 3, the radius r and center of circle spacing of two white circle lighting sources of control LED array 1
D is to be capable of achieving the flexible adjustable of deep and stereoscopic angle of focusing.Assuming that the radius of left side white circle lighting source is r, the center of circle
At A points;The radius of the right white circle lighting source is r, and the center of circle is located at B points, and O points are the midpoint of AB,
Positioned at microcobjective, the central axis of lens.OC is the distance of P4 series LEDs array 1 and sample stage 2,
Represented with h, generally 80mm or so.Can be realized to body view angle theta for d by controlling two horizontal intervals in the center of circle
Control:θ=arctand/2h;Can be realized for illumination numerical aperture angle θ by controlling the radius r in two centers of circleNAControl
System:θNA≈ θ '=arctan r/h, numerical aperture angle θNAIt is inversely proportional to depth of focus.
Preferably, the CCD camera lenses of video camera 5 are located on the back focal plane of tube lens 4.
Further, the computer 7 sends to the trigger signal that video camera 5 sends with to P4 series LEDs array 1
Trigger signal keep synchronous.
The stereoscopic micro imaging method of polarization type dynamic realtime of the present invention, step is as follows:
Step 1, sample stage 2 is placed in by sample to be imaged, and computer 7 sends to P4 series LEDs array 1
Trigger signal, makes P4 series LEDs array 1 be alternately produced two circular illumination light;Two radiuses of circular illumination light
For r, center of circle spacing are that d, color are identical, wherein r, d are the number of pixel.
Step 2, the illumination light that step 1 is produced is collected through sample stage 2 by microcobjective 3, and microcobjective 3 will
The illumination light of collection is incident to tube lens 4 after being amplified imaging.
Step 3, computer 7 sends trigger signal and passes through tube lens 4 for 5 pairs with actuated camera to actuated camera 5
Sample image sampled, obtain corresponding respectively to two microobject images of circular illumination light;The computer 7
The trigger signal that the trigger signal sent to video camera 5 sends with step 1 Computer 7 to P4 series LEDs array 1
Keep synchronous.
Step 4, two 6 points of microobject image input polarization formula 3D displays that video camera 5 photographs step 3
Not shown as left-and right-eye images;
Step 5, observer watches in real time by watching polarization type 3D displays 6 by wearing polarization type 3D glasses 8
The stereomicroscopy structure of sample image.Fig. 4 is the sample drawing that shot by camera is arrived in the present embodiment.
The use of P4 series LEDs array in the present invention, instead of the microscopical double light path of conventional stereo, simplify system
Design, not only makes imaging system easy but also effective reduces cost;It is used as lighting source by using LED array, has
There is system light source in itself, the restricted reduction of observation condition smaller by external interference can be seen under dark background
See;Focusing is realized apart from d by the radius r and the center of circle that control the circular illumination light source on P4 series LED arrays respectively
Deep and stereoscopic angle it is flexible adjustable;By camera acquisition to image pass on polarization type 3D displays, such that it is able to reality
Now the dynamic realtime to sample stereochemical structure is clearly watched, and overcomes the shortcoming of the color effects difference of red blue three-dimensional imaging, lifting
Observer's Experience Degree.
Claims (9)
1. a kind of stereoscopic microscopic imaging device of polarization type dynamic realtime, it is characterised in that including P4 series LED arrays
(1), sample stage (2), microcobjective (3), tube lens (4), video camera (5), polarization type 3D show
Device (6), computer (7) and polarization type 3D glasses (8);Wherein P4 series LEDs array (1), sample stage
(2), microcobjective (3), tube lens (4), video camera (5) set gradually from bottom to up, and P4 series LEDs
Array (1) is centrally located on microcobjective (3), the central axis of tube lens (4);P4 series LED arrays
(1) it is connected with computer (7) with the control end of video camera (5), the output end of video camera (5) accesses polarization type
3D displays (6);
Sample to be imaged is placed in sample stage (2), computer (7) sends to P4 series LEDs array (1)
Trigger signal, makes P4 series LEDs array (1) be alternately produced two circular illumination light, and illumination light passes through sample loading
Platform (2) is collected by microcobjective (3), and microcobjective (3) is incident to after the illumination light of collection is amplified into imaging
Tube lens (4), computer (7) sends trigger signal with actuated camera (5) to wearing to actuated camera (5)
The sample image for crossing tube lens (4) is sampled, the sample image input polarization formula 3D that video camera (5) will be gathered
Display (6) is shown that observer watches polarization type 3D displays (6) by wearing polarization type 3D glasses (8)
The stereomicroscopy structure of sample image can in real time be watched.
2. the stereoscopic microscopic imaging device of polarization type dynamic realtime according to claim 1, it is characterised in that described
P4 series LEDs array (1) provide red, green, blueness, cyan, pink colour, yellow, seven kinds of photographs of color of white light
Mingguang City, the present apparatus uses random color illumination light, and two circular illumination light source colours are identical;P4 series LEDs
The unit board size of array is 128mm*128mm, number of pixels is 32*32, pel spacing 4mm, each pixel
Can individually light.
3. the stereoscopic microscopic imaging device of polarization type dynamic realtime according to claim 1, it is characterised in that described
Distance of P4 series LEDs array (1) apart from sample stage (2) upper surface is 75~85mm.
4. the stereoscopic microscopic imaging device of polarization type dynamic realtime according to claim 1, it is characterised in that described
Computer (7) sends trigger signal to P4 series LEDs array (1), makes P4 series LEDs array (1) alternately
It is d, color identical circular illumination light source for r, center of circle spacing to produce two radiuses, and wherein r, d is pixel number.
5. the stereoscopic microscopic imaging device of polarization type dynamic realtime according to claim 1, it is characterised in that described
The CCD camera lenses of video camera (5) are located on the back focal plane of tube lens (4).
6. the stereoscopic microscopic imaging device of polarization type dynamic realtime according to claim 1, it is characterised in that described
Computer (7) is believed to video camera (5) trigger signal for sending and the triggering sent to P4 series LEDs array (1)
Number keep synchronous.
7. a kind of stereoscopic micro imaging method of polarization type dynamic realtime based on claim 1 described device, its feature exists
In step is as follows:
Step 1, sample stage (2) is placed in by sample to be imaged, and computer (7) is to P4 series LEDs array (1)
Trigger signal is sent, P4 series LEDs array (1) is alternately produced two circular illumination light;
Step 2, the illumination light that step 1 is produced is collected through sample stage (2) by microcobjective (3), micro- thing
Mirror (3) is incident to tube lens (4) after the illumination light of collection is amplified into imaging;
Step 3, computer (7) sends trigger signal with actuated camera (5) to through mirror to actuated camera (5)
The sample image of cylinder lens (4) is sampled, and obtains corresponding respectively to two microobject images of circular illumination light;
Step 4, two microobjects image input polarization formula 3D displays (6) that video camera (5) photographs step 3
Shown respectively as left-and right-eye images;
Step 5, observer by wear polarization type 3D glasses (8) watch polarization type 3D displays (6) can be real-time
Watch the stereomicroscopy structure of sample image.
8. the stereoscopic micro imaging method of polarization type dynamic realtime according to claim 7, it is characterised in that step 1
The P4 series LEDs array (1) is alternately produced two circular illumination light, two radiuses of circular illumination light be r,
Center of circle spacing is d, color is identical, and wherein r, d are the number of pixel.
9. the stereoscopic micro imaging method of polarization type dynamic realtime according to claim 7, it is characterised in that step 3
The trigger signal that the computer (7) is sent to video camera (5) is with step 1 Computer (7) to P4 series LEDs
The trigger signal that array (1) sends keeps synchronous.
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Cited By (1)
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CN110955039A (en) * | 2019-11-15 | 2020-04-03 | 上海安翰医疗技术有限公司 | Phase contrast microscopic imaging system and imaging method thereof |
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