CN106771138A - A kind of anti-SS A IgG antibody chemiluminescence immune detection reagent kits and preparation method thereof - Google Patents
A kind of anti-SS A IgG antibody chemiluminescence immune detection reagent kits and preparation method thereof Download PDFInfo
- Publication number
- CN106771138A CN106771138A CN201611018735.2A CN201611018735A CN106771138A CN 106771138 A CN106771138 A CN 106771138A CN 201611018735 A CN201611018735 A CN 201611018735A CN 106771138 A CN106771138 A CN 106771138A
- Authority
- CN
- China
- Prior art keywords
- preparation
- acridinium ester
- igg
- antibody
- kit according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The invention discloses a kind of anti-SS A IgG antibody chemiluminescence immune detection reagent kits, the kit includes：The coated magnetic particle of SS Staphylococal Protein As, the coated acridinium ester of anti-human igg monoclonal antibody, anti-SS A IgG antibodies calibration product, preexciting liquid, exciting liquid.In addition the invention also discloses a kind of preparation method of anti-SS A IgG antibody chemiluminescence immune detection reagent kits.Kit of the present invention is easy to operate compared with available reagent box, and sensitivity is high, the advantages of detection range is wide.
Description
Technical field
The present invention relates to in-vitro diagnosis field of immunodetection, specifically, the invention provides a kind of inspection of chemiluminescence immunoassay
Survey Anti SS-A antibody IgG kits and preparation method thereof.
Background technology
SS-A IgG（Also referred to as Anti-Ro, or Anti SS-A antibody IgG）It is a kind of ribonucleoprotein, is distributed widely in all
The RNA protein bodies of tissue, it is made up of two kinds of albumen（60kDa and 52kDa）.Anti- SS-A antibody is found in drying first
In the serum of syndrome patient.
SS-A may occur in which in different CTDs, such as systemic loupus erythematosus（30%-50%）, Sjogren syndrome（50-
80%）, PBC (20%) and neonatal lupus syndrome（NLE）（95%-100%）, Subacute Cutaneous Lupus are red
Yabbi sore（SCLE）（60%）, rheumatoid arthritis（3%-5%）Deng.And it is closely related with some special clinical manifestations, for example
Photosensitivity rash, xerosis, hypergammaglobulinemia purpura etc..If there is SS-A in the pregnant woman for suffering from systemic loupus erythematosus
IgG is exceeded, then it represents that the possibility that baby suffers from neonatal erythema lupus increases.
The main method of clinical detection Anti SS-A antibody IgG be enzyme linked immunosorbent assay, but the method exist it is following
Weak point：
（1）Using 12 × 8 types, 6 × 8 types, 8 × 12 types or the hole Special micro porous plate of complete plate 96 as antigen coat apparatus and instead
Container is answered, 12 batches, 6 batches, 8 batches or whole plate first use can only be divided into when in use, it is impossible to carry out independent, single part
Detection；
（2）Quantitative determination reagent type used is more, and each detection reagent will be contained with reagent bottle, and often be made
It is required for changing imbibition nozzle during with a kind of reagent being filled into the micropore of microwell plate respectively, not only reagent bottle species is more, filling
The operation of reagent is also extremely cumbersome；
（3）Lack the corresponding mark to detection information, can only just will appreciate that or know by checking the mark of kit external packing box
The product batch number and term of validity information of detection reagent are known, and the information known is uncontrolled in detection process, with very big
Randomness；
（4）Detection reagent, in open space, easily causes the cross pollution between various reagents and shadow in detection process
Ring the accuracy of testing result；
（5）Using manual operations more than detection process, the dosage of reagent or sample is not bery accurate, and operating process is extremely cumbersome and multiple
It is miscellaneous, bust is susceptible to, the degree of accuracy of testing result and precision are poor；
（6）In the quantity configuration of detection project reagent set and using item number × 48/96 person-portion is above, if necessary to examine
10 projects are surveyed, then the configuration of reagent and the use of number must be 10 × 48/96 person-portions, if only a sample needs detection 10
Different project, it is also desirable to configure the reagent of 10 × 48/96 person-portions, haves the shortcomings that inadequate economical rationality.
The content of the invention
Current Anti SS-A antibody IgG detection techniques have the following disadvantages：Testing cost is high, detection sensitivity is low, detection line
Property narrow range, reappearance is low, can not quantify, complex operation etc..
The present invention discloses that a kind of testing cost is low, sensitivity is high, detection is linear precisely in order to overcome the above shortcoming
Scope is wide, reappearance is high, can quantify, Anti SS-A antibody IgG kits simple to operate and preparation method thereof.It is of the invention first
Chemical luminescence immune analysis reagent box is first prepared, is mainly included：It is the coated magnetic particle of Anti SS-A antibody IgG monoclonal antibody, anti-
The coated acridinium ester of SS-A IgG antibody monoclonal antibodies and Anti SS-A antibody IgG calibration product；Then full-automatic chemical is utilized
Luminescence immunoassay instrument detects that drafting standard curve is built in computer software, tests actual sample to calibration product, according to
Sample luminous value calculates concentration of specimens；Finally confrontation SS-A IgG antibody automatic chemiluminescence immunoassay systems carry out performance
（Sensitivity, linear, precision, interference）Evaluation.
It is of the invention compared with current technology, with advantages below：
1st, present invention selection acridinium ester is used as marker material, and is applied to chemiluminescence immunoassay system, and the luminescence system is
Direct chemiluminescence, compared with traditional enzyme-catalyzed chemical luminescence, the reaction does not need the participation of enzyme, more cost-effective；
2nd, the acridinium ester chemiluminescent immunoassay system detection sensitivity that the present invention is selected is high, can reach 0.2 AU/mL, phase
10 times are at least improve than the sensitivity of other Anti SS-A antibody IgG detection methods；
3rd, the acridinium ester chemiluminescent immunoassay system range of linearity that the present invention is selected is wide, can reach 3-800 AU/mL, other
Anti SS-A antibody IgG chemistry hair detection method the inspection range of linearity be 20-150 AU/mL；
4th, the acridinium ester chemiluminescent immunoassay system repeatability that the present invention is selected is high, in batch and difference between batch is within 5%,
This is that other chemiluminescence immunoassay systems are unapproachable；
5th, chemiluminescence immunoassay system of the invention has realized quantifying for sample, soft to testing by built-in standard curve
Part, only needs test sample to directly obtain the concentration value of sample；
6th, chemiluminescence immunoassay system of the invention has realized full-automation, and the addition of reagent and sample has instrument complete entirely
Into operation is easier, reduces artificial error.
Brief description of the drawings
Fig. 1 is the Anti SS-A antibody IgG canonical plottings that embodiment 3 is obtained.
Specific embodiment
Embodiment 1：Anti SS-A antibody IgG chemiluminescence immune detection reagent kit preparation methods
（1）It is prepared by the nanometer magnetic bead of SS-A antigen coats：
Take the magnetic particle of 50mg carboxylated（Particle diameter is 0.05-1um）Suspension, Magneto separate removes supernatant, and it is 5.5 to use 0.02 M, pH
MES buffer solutions are resuspended, add the EDC aqueous solution of 10 mg/mL of the new configurations of 0.5-2mL, activated magnetic beads surface carboxyl groups to add 3-5
Mg SS-A antigens, suspension 2-10 h, Magneto separate, remove supernatant, with the Tris that the 0.1 M pH containing 2% BSA are 8.0 at room temperature
Buffer solution is resuspended to 1mg/mL, obtains the magnetic particle of SS-A antigen coats, every bottle of 5mL packing be stored in 4 DEG C it is standby.
（2）Anti-human igg derives the preparation of the acridinium ester of substance markers：
The anti-human igg derivative of 50 uL 25mg/mL is taken, adds the carbonate of 150 uL 0.1-0.2 M pH 9.0-9.5 to delay
Fliud flushing, is mixed, and the acridinium ester for being subsequently adding the mg/mL of 1-2 uL 5 is mixed, at room temperature lucifuge reaction, is taken out after 1-2 h, with 2
The zeba centrifugation desalting column desalting processings of mL, are processed, finally with pure water and TBS buffer solutions respectively first in desalination processes
The acridine ester solution of the anti-human igg derivative mark that addition is obtained, liquid to the preservation collected in centrifuge tube is in control
The acridinium ester of anti-human igg derivative mark, every bottle of 5 mL packing be stored in 4 DEG C it is standby.
（3）Anti SS-A antibody IgG calibrates the preparation of product：
Use standard items buffer solution（40 mM Tris-HCl, 0.5% BSA, 1% NaCl, pH 8.0）Anti SS-A antibody IgG is matched somebody with somebody
Concentration is set to for 0 AU/mL, 5 AU/mL, 20 AU/mL, 100 AU/mL, 400 AU/mL, 800 AU/mL, every bottle 0.5 mL points
Dress is lyophilized, and 4 DEG C save backup.
(4) preparation of chemiluminescence preexciting liquid：
1.0 liters of purified waters are measured, the hydrogen peroxide (H that 80uL mass fractions are 20% is sequentially added2O2), 1.0 grams of sodium azide, 1.5
Gram polysorbas20, shakes up rear lucifuge storage.
(5) preparation of chemiluminescence exciting liquid：
1.0 liters of purified waters are measured, 0.6 gram of NaOH, 0.5 gram of PC300,0.5g sodium azide, 1.5 grams of Triton are sequentially added
405, shake up rear lucifuge storage.
Embodiment 2：Anti SS-A antibody IgG chemical luminous immune detection methods：
The present invention is detection instrument with Full-automatic chemiluminescence immunoassay analysis meter, and method of the present invention pattern is indirect method, i.e.,
Instrument sequentially adds the anti-human igg derivative mark of the sample of 20 uL, the magnetic particle of the SS-A antigen coats of 50 uL and 50 uL
Remember coated acridinium ester, after 10 min of reaction, carry out Magneto separate, reactant mixture is sent into darkroom by instrument, sequentially adds 50uL
Chemiluminescence preexciting liquid, 50uL chemiluminescences exciting liquid carry out luminescence-producing reaction, finally record luminous intensity, from standard curve meter
Calculate the Anti SS-A antibody IgG content of sample.
Embodiment 3：Anti SS-A antibody IgG chemiluminescence immune detection reagent kit performance evaluations
Detection curve is shown in accompanying drawing 1.
The detection of sensitivity：
With reference to CLSI EP17-A file recommendation experimental programs, Anti SS-A antibody IgG chemiluminescence immunoassays chromatography kit is calculated
Sensitivity, the sensitivity tried to achieve be 0.2 AU/mL.
Linear detection：
It is that 0 AU/mL, 5 AU/mL, 20 AU/mL, 100 AU/mL, 400 AU/mL, 800 AU/mL standard items make line to concentration
Property analysis, calculate linearly dependent coefficient, r=0.9998, in addition, the kit confrontation SS-A IgG antibody sample detections it is linear
Scope is 3-800 AU/mL.
Precision is determined：
Concentration is taken for two Anti SS-A antibody IgG samples of 2 AU/mL and 200 AU/mL, each sample each concentration respectively does 3
It is parallel, detected with three batches of kits, calculate in kit batch and difference between batch, as a result show in the kit batch and difference between batch
Respectively less than 5%.
Interference is tested：
Taking pooled serum and adding chaff interference respectively includes：It is combined with bilirubin, unconjugated bilirubin, hemoglobin, ascorbic acid, sweet
Grease, adding proportion is according to 1:20 are carried out, and are determined pooled serum respectively and be with the addition of the survey of pooled serum after various chaff interferences
Value, calculates deviation therebetween, with ± 10% for tolerance interval.Result shows that interference reaches the file of NCCLS
Standard, can be used for the accurate evaluation of clinical labororatory's Anti SS-A antibody IgG situations.
Embodiment 4:The Sensitivity comparison experiment of Anti SS-A antibody IgG chemiluminescence immune detection reagent kits
It is respectively the calibration object or sample of 0 AU/mL to concentration with chemical luminescence detection method and traditional enzyme linked immunosorbent assay
Dilution is detected that replication 20 times draws 20 RLU values of measurement result for sample（Relative light unit）, calculate it
Average value（M）And standard deviation（SD）, M+2SD is drawn, luminous value substitution calibration curve is calculated corresponding concentration value.Adopt
The concentration value obtained with chemical luminescence detection method is 0.2 AU/mL, relative to traditional enzyme linked immunosorbent assay lowest detection
2.0 AU/mL are limited, about 10 times are improve.
Claims (10)
1. a kind of Anti SS-A antibody IgG chemiluminescence immune detection reagent kits, the kit includes：SS-A antigen coats-
Nanometer magnetic microsphere, chemiluminescent labels, Chemoluminescent substrate, Anti SS-A antibody IgG calibration product.
2. kit according to claim 1, it is characterised in that the solid phase carrier of the SS-A antigen coats is that magnetic is micro-
Grain.
3. kit according to claim 1, it is characterised in that the solid phase carrier of the SS-A antigen coats is carboxylated
Particle diameter be 0.05-1um magnetic particles.
4. kit according to claim 1, it is characterised in that the chemiluminescent labels are acridinium ester, acridinium ester
Sulfonamide, acridinium ester toluenesulfonamide, acridinium ester are to methylsulfonamides, acridinium ester trimethyl fluoride sulfonyl amine.
5. kit according to claim 1, it is characterised in that the preferred acridinium ester of chemiluminescent labels.
6. kit according to claim 1, it is characterised in that the Chemoluminescent substrate is excited including chemiluminescence
Liquid, chemiluminescence preexciting liquid.
7. kit according to claim 1, it is characterised in that the chemiluminescence preexciting liquid is mass fraction
0.005% ~ 0.5% hydrogen peroxide (H2O2) solution, exciting liquid is the NaOH of 0.005mol/L ~ 0.025mol/L
(NaOH) solution.
8. kit according to claim 1, it is characterised in that the Anti SS-A antibody IgG calibrations product are to use standard items
Anti SS-A antibody IgG is configured to concentration for 0 AU/mL, 5 AU/mL, 20 AU/mL, 100 AU/mL, 400 AU/ by buffer solution
ML, 800 AU/mL, 4 DEG C save backup.
9. kit according to claim 1, it is characterised in that the preparation method of the kit, it is characterised in that bag
Include preparation, chemistry hair that the preparation of the magnetic particle of SS-A antigen coats, anti-human igg monoclonal antibody derive the acridinium ester of substance markers
The preparation of product is calibrated in the preparation of light substrate solution, Anti SS-A antibody IgG.
10. according to claim 1 and claim 9 kit preparation method, it is characterised in that comprise the following steps：
1）The preparation of the magnetic particle of SS-A antigen coats：
The nanometer magnetic bead suspension of carboxylated is taken, Magneto separate removes supernatant, and MES buffer solutions are resuspended, add the EDC aqueous solution, activate magnetic
Bead surface carboxyl, adds SS-A antigens, at room temperature suspension 2-10 h, and Magneto separate removes supernatant, and Tris buffer solutions are resuspended, obtain
The magnetic particle of SS-A antigen coats；Optionally, a diameter of 0.1 μm ~ 2.0 μm of carboxylated nanometer magnetic bead；
MES buffer concentrations are 10mM ~ 100mM, pH 5.5 ~ 8.5；
2）The preparation of the acridinium ester of anti-human igg monoclonal antibody：
Anti-human igg monoclonal antibody derivative is taken, carbonate buffer solution is added, mixed, be subsequently adding acridinium ester mixing, at room temperature
Lucifuge is reacted, and is taken out after 1-2 h, and desalting column desalting processing is centrifuged, and uses pure water and TBS buffer solutions in desalination processes respectively first
Processed, the acridine ester solution that the anti-human igg monoclonal antibody for obtaining derives substance markers is eventually adding, in collection centrifuge tube
Liquid is in control the acridinium ester of anti-human igg monoclonal antibody derivative substance markers to preserving；
3）Anti SS-A antibody IgG calibrates the preparation of product：
Anti SS-A antibody IgG is configured to concentration for 0 AU/mL, 5 AU/mL, 20 AU/mL, 100 with standard items buffer solution
AU/mL, 400 AU/mL, 800 AU/mL, packing are lyophilized, and 4 DEG C save backup；
4）The preparation of chemiluminescence preexciting liquid：
1.0 liters of purified waters are measured, the hydrogen peroxide (H that 0.5 ~ 100uL mass fractions are 20% is sequentially added2O2), 0.5 ~ 5 gram prevent
Rotten agent, 0.5 ~ 5 gram of surfactant, shake up rear lucifuge storage；Optionally, preservative be commercialization sodium azide, PC300,
Surfactant is polysorbas20, Tween 80, Triton X100, Triton 405；
5）The preparation of chemiluminescence exciting liquid：
1.0 liters of purified waters are measured, 0.2 ~ 1 gram of NaOH, 0.5 ~ 5 gram of preservative, 0.5 ~ 5 gram of surface is sequentially added and is lived
Property agent, shake up the storage of rear lucifuge；Optionally, preservative is commercialization sodium azide, PC300, and surfactant is polysorbas20, tells
Temperature 80, Triton X100, Triton 405.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620676845 | 2016-06-30 | ||
| CN2016206768457 | 2016-06-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106771138A true CN106771138A (en) | 2017-05-31 |
Family
ID=58969658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611018735.2A Pending CN106771138A (en) | 2016-06-30 | 2016-11-21 | A kind of anti-SS A IgG antibody chemiluminescence immune detection reagent kits and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106771138A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101750492A (en) * | 2008-12-04 | 2010-06-23 | 上海裕隆生物科技有限公司 | Self-immunity hepatitis detection protein chip and kit thereof |
| CN103954779A (en) * | 2014-03-12 | 2014-07-30 | 长春迪瑞医疗科技股份有限公司 | Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology |
| CN104897901A (en) * | 2015-05-12 | 2015-09-09 | 西安金磁纳米生物技术有限公司 | Goldmag particle-based acridinium ester chemiluminescence immunological detection method of HE4 |
| CN105445474A (en) * | 2015-11-17 | 2016-03-30 | 苏州浩欧博生物医药有限公司 | Anti-SS-A(Ro60) antibody kit and detection method thereof |
| CN105445456A (en) * | 2015-11-16 | 2016-03-30 | 北京中航赛维生物科技有限公司 | Kit for quantitatively detecting anti-SS-A antibody IgG by utilizing magnetic particle chemiluminescence, preparation method and detection method thereof |
-
2016
- 2016-11-21 CN CN201611018735.2A patent/CN106771138A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101750492A (en) * | 2008-12-04 | 2010-06-23 | 上海裕隆生物科技有限公司 | Self-immunity hepatitis detection protein chip and kit thereof |
| CN103954779A (en) * | 2014-03-12 | 2014-07-30 | 长春迪瑞医疗科技股份有限公司 | Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology |
| CN104897901A (en) * | 2015-05-12 | 2015-09-09 | 西安金磁纳米生物技术有限公司 | Goldmag particle-based acridinium ester chemiluminescence immunological detection method of HE4 |
| CN105445456A (en) * | 2015-11-16 | 2016-03-30 | 北京中航赛维生物科技有限公司 | Kit for quantitatively detecting anti-SS-A antibody IgG by utilizing magnetic particle chemiluminescence, preparation method and detection method thereof |
| CN105445474A (en) * | 2015-11-17 | 2016-03-30 | 苏州浩欧博生物医药有限公司 | Anti-SS-A(Ro60) antibody kit and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106855572A (en) | A kind of gastrin-releasing peptide precursor chemiluminescence immune detection reagent kit and preparation method thereof | |
| WO2018000898A1 (en) | Zinc transporter protein 8 antibody chemiluminescence immunoassay kit and preparation method therefor | |
| JPH0664059B2 (en) | Method for measuring analyte concentration | |
| CN106442970A (en) | Anti-double-stranded DNA antibodies IgG chemiluminescence immunoassay kit and preparation method thereof | |
| CN107044977A (en) | A kind of tyrosine phosphatase antibody chemical luminescence immunity detection reagent and preparation method thereof | |
| CN108508001A (en) | Chemiluminescence detection kit | |
| CN109239367A (en) | Measure the method and kit of small molecule compound | |
| CN106645738A (en) | Anti-cyclic citrullinated peptide antibody chemiluminescence immune detection kit and preparation method thereof | |
| CN106645711A (en) | Anti-Sm antibody IgG determining kit (chemiluminiscence method) and preparation method thereof | |
| CN106053440A (en) | Mycoplasma pneumoniae IgG chemiluminescence immunoassay kit and preparation method thereof | |
| CN106645689A (en) | Thyroid-stimulating hormone receptor antibody chemiluminescent immunoassay kit and preparation method thereof | |
| CN106248944A (en) | A kind of CPn IgG chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN105954509A (en) | Renin chemiluminescence immunoassay kit and preparation method thereof | |
| CN106596919A (en) | Kit (chemiluminiscence method) for determining anti-ribonucleoprotein 70 antibody IgG and manufacturing method thereof | |
| CN107966563A (en) | A kind of antimyeloperoxidase antibody IgG chemiluminescence immunoassay kits and preparation method thereof | |
| CN105911296A (en) | IV-type collagen chemiluminescence immunoassay kit and preparation method thereof | |
| US5721105A (en) | Method for the immunological determination of proteins and kit for carrying out the method | |
| CN107102140A (en) | A kind of glutamic acid decarboxylase antibody chemical luminescence immunity detection reagent and preparation method thereof | |
| CN109073641A (en) | Use the Radioimmunoassay of vascular endothelial growth of sulfated polysaccharides class | |
| CN106645759A (en) | Aldosterone chemiluminescent immunodetection kit and preparation method thereof | |
| CN106404754A (en) | A chlamydiae pneumoniae IgM chemiluminescent immunoassay kit and a preparing method thereof | |
| CN106248943A (en) | Antinuclear antibody chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN106198998A (en) | Human a-fetoprotein heteroplasmon 3 chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN106855574A (en) | A kind of III procollagen type N-terminal peptide chemiluminescence immunity detection reagent and preparation method thereof | |
| CN106199012A (en) | Inhibin B chemiluminescence immune detection reagent kit and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |