CN106770910A - A kind of assay method of organic acid content - Google Patents
A kind of assay method of organic acid content Download PDFInfo
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- CN106770910A CN106770910A CN201611055238.XA CN201611055238A CN106770910A CN 106770910 A CN106770910 A CN 106770910A CN 201611055238 A CN201611055238 A CN 201611055238A CN 106770910 A CN106770910 A CN 106770910A
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- organic acid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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Abstract
The invention belongs to analytical chemistry field, more particularly to a kind of assay method of organic acid content.A kind of assay method of organic acid content, comprises the following steps：(1) 0.30g of benzoic acid 0.24 is weighed, is added into 250mL conical flasks；(2) 20 30mL originally water dissolves are subsequently adding；(3) phenolphthalein indicator 12 is added to drip；(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；NaOH consumptions are write down, parallel titration 3 times calculates organic acid mass fraction w.The detection method that the present invention is provided is capable of the content of Accurate Determining organic acid；The detection method that the present invention is provided is workable, with low cost.
Description
Technical field
The invention belongs to analytical chemistry field, more particularly to a kind of assay method of organic acid content.
Background technology
Organic acid (Organic acids) is the compound containing carboxyl (- COOH) in molecular structure.In Chinese herbal medicine
It is widely distributed in leaf, particularly root, fruit, such as dark plum, the fruit of Chinese magnoliavine, raspberry etc..Organic acid in common plant has fat
Unitary, binary, the polybasic carboxylic acid of race such as tartaric acid, oxalic acid, malic acid, citric acid, ascorbic acid (i.e. vitamin C) etc., also have
Aromatic organic acid such as benzoic acid, salicylic acid, caffeic acid (Caffelc acid) etc..In addition to minority exists with free state, one
As be all combined into salt with potassium, sodium, calcium etc., some are combined into salt with alkaloids.Aliphatic acid is more and glycerine be combined into ester or with height
Level alcohol is combined into wax.Some organic acids are the constituents of volatile oil and resin.
Most of organic acids are weak acid, if certain organic acid is soluble in water, dissociation constant >=10-7, can be straight with standard base (SB)
Connect and survey its content, product is strong base-weak acid salt.
The information for being disclosed in the background section is merely intended to increase the understanding to general background of the invention, without answering
In being considered as recognizing or imply in any form that the information structure has been the prior art well known to persons skilled in the art.
The content of the invention
In order to overcome the defect of prior art, the invention provides a kind of assay method of organic acid content, the method inspection
Degree of testing the speed is fast, easy to operate.
To achieve these goals, the present invention is realized using following technical scheme：
A kind of assay method of organic acid content, comprises the following steps：
(1) benzoic acid 0.24-0.30g is weighed, is added into 250mL conical flasks；
(2) 20-30mL originally water dissolves are subsequently adding；
(3) phenolphthalein indicator 1-2 drops are added；
(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；Write down NaOH use
Amount, parallel titration 3 times, calculates organic acid mass fraction w.
Preferably, phenolphthalein indicator is 0.2% ethanolic phenolphthalein solution in step (3).
Preferably, the concentration of NaOH standard liquids is 0.1mol/L in step (4).
Compared with prior art, the present invention has the advantages that：
(1) detection method that the present invention is provided is capable of the content of Accurate Determining organic acid.
(2) detection method that the present invention is provided is workable, with low cost.
Specific embodiment
Specific embodiment of the invention is described in detail with reference to specific embodiment, it is to be understood that of the invention
Protection domain do not limited by specific embodiment.
Embodiment 1：
A kind of assay method of organic acid content, comprises the following steps：
(1) benzoic acid 0.24g is weighed, is added into 250mL conical flasks；
(2) 20mL originally water dissolves are subsequently adding；
(3) phenolphthalein indicator 1 is added to drip；Described phenolphthalein indicator is 0.2% ethanolic phenolphthalein solution；
(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；Write down NaOH use
Amount, parallel titration 3 times, calculates organic acid mass fraction w；The concentration of NaOH standard liquids is 0.1mol/L in step (4).
Embodiment 2：
A kind of assay method of organic acid content, comprises the following steps：
(1) benzoic acid 0.30g is weighed, is added into 250mL conical flasks；
(2) 30mL originally water dissolves are subsequently adding；
(3) phenolphthalein indicator 2 is added to drip；Described phenolphthalein indicator is 0.2% ethanolic phenolphthalein solution；
(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；Write down NaOH use
Amount, parallel titration 3 times, calculates organic acid mass fraction w；The concentration of NaOH standard liquids is 0.1mol/L in step (4).
Embodiment 3：
A kind of assay method of organic acid content, comprises the following steps：
(1) benzoic acid 0.26g is weighed, is added into 250mL conical flasks；
(2) 25mL originally water dissolves are subsequently adding；
(3) phenolphthalein indicator 1 is added to drip；Described phenolphthalein indicator is 0.2% ethanolic phenolphthalein solution；
(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；Write down NaOH use
Amount, parallel titration 3 times, calculates organic acid mass fraction w；The concentration of NaOH standard liquids is 0.1mol/L in step (4).
The foregoing description to specific illustrative embodiment of the invention be in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explaining that certain principles of the invention and its reality should
With so that those skilled in the art can realize and using a variety of exemplaries of the invention and
A variety of selections and change.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (3)
1. a kind of assay method of organic acid content, it is characterised in that comprise the following steps：
(1) benzoic acid 0.24-0.30g is weighed, is added into 250mL conical flasks；
(2) 20-30mL originally water dissolves are subsequently adding；
(3) phenolphthalein indicator 1-2 drops are added；
(4) solution is titrated in blush with NaOH standard liquids, if colour-fast in 30s, as terminal；NaOH consumptions are write down,
Parallel titration 3 times, calculates organic acid mass fraction w.
2. the assay method of organic acid content according to claim 1, it is characterised in that phenolphthalein indicator in step (3)
It is 0.2% ethanolic phenolphthalein solution.
3. the assay method of organic acid content according to claim 1, it is characterised in that NaOH standards are molten in step (4)
The concentration of liquid is 0.1mol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611055238.XA CN106770910A (en) | 2016-11-25 | 2016-11-25 | A kind of assay method of organic acid content |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611055238.XA CN106770910A (en) | 2016-11-25 | 2016-11-25 | A kind of assay method of organic acid content |
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| CN106770910A true CN106770910A (en) | 2017-05-31 |
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| CN201611055238.XA Pending CN106770910A (en) | 2016-11-25 | 2016-11-25 | A kind of assay method of organic acid content |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858275A (en) * | 2021-01-25 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Method for rapidly detecting sodium glutamate in monosodium glutamate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940947A (en) * | 2014-04-30 | 2014-07-23 | 江苏国泰环境监测有限公司太仓分公司 | Detection method of sulfides in wastewater |
| CN104215737A (en) * | 2014-09-01 | 2014-12-17 | 梁山正大菱花生物科技有限公司 | Revertose detection method |
-
2016
- 2016-11-25 CN CN201611055238.XA patent/CN106770910A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940947A (en) * | 2014-04-30 | 2014-07-23 | 江苏国泰环境监测有限公司太仓分公司 | Detection method of sulfides in wastewater |
| CN104215737A (en) * | 2014-09-01 | 2014-12-17 | 梁山正大菱花生物科技有限公司 | Revertose detection method |
Non-Patent Citations (3)
| Title |
|---|
| 张秀梅等: "《涂料工业用原材料技术标准手册》", 31 December 2004 * |
| 李莉等: "《分析化学实验》", 30 April 2016 * |
| 马东平等: "饮料中苯甲酸含量的测定", 《陇东学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858275A (en) * | 2021-01-25 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Method for rapidly detecting sodium glutamate in monosodium glutamate |
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Application publication date: 20170531 |