CN106770870A - The detection method of reductive glutathione content in a kind of wheat embryo - Google Patents
The detection method of reductive glutathione content in a kind of wheat embryo Download PDFInfo
- Publication number
- CN106770870A CN106770870A CN201611107105.2A CN201611107105A CN106770870A CN 106770870 A CN106770870 A CN 106770870A CN 201611107105 A CN201611107105 A CN 201611107105A CN 106770870 A CN106770870 A CN 106770870A
- Authority
- CN
- China
- Prior art keywords
- detection method
- sample
- high performance
- content
- liquid chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/884—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample organic compounds
Landscapes
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention provides a kind of detection method of reductive glutathione content in wheat embryo.The present invention obtains the content of reductive glutathione content in wheat embryo using the high performance liquid chromatography with UV-detector using calibration curve method, so as to obtain measuring accuracy higher, may be adapted to the detection of reductive glutathione content in Food and hygienical food.
Description
Technical field
The present invention relates to the technical field of glutathione content detection, reproducibility paddy Guang in a kind of wheat embryo is particularly related to
The detection method of sweet peptide content.
Background technology
At present, for reduced glutathione detection method AAS, there are enzyme process, fluorescence method, electrochemical gaging
Method, infrared detection analytic approach etc..More conventional method is exactly AAS, but spectrophotometry out be paddy
The total amount of the sweet peptide of Guang, detection is not reductive glutathione content, although many people are improving this technology and are being born
Many Patents, but this problem is unable to essence solution.Other more common methods be based on Ellman reagents (5,5 '-
Two thiobis (2- nitrobenzoic acids), DTNB) with sulfydryl reaction DTNB detection methods, but the method is made due to evaluated error
Accuracy is very limited, and the glutathione reductase round-robin method set up based on the method needs to use expensive auxiliary
Enzyme, using also having limited.The reaction speed of fluorescence method is fast, sensitivity is high, but being easily disturbed also has it to limit to；Electrochemistry is surveyed
Determining method also has relatively costly grade of electrode fabrication to limit.
In the prior art, although established the method for many measure reduced glutathione, but the base for detecting
This is the total amount of glutathione content, detects that inaccurate or precision is relatively low.
The content of the invention
In view of this, the present invention provides a kind of detection method of reductive glutathione content in wheat embryo, the detection
Method precision is higher.
The detection method of reductive glutathione content in a kind of wheat embryo, uses the efficient liquid phase with UV-detector
Chromatogram obtains the content of reductive glutathione content in wheat embryo using calibration curve method.
Above-mentioned high performance liquid chromatography (HPLC) generally includes reservoir, infusion pump, blender, sampling device, separation chromatography
Post, the component of detection means five.The above-mentioned high performance liquid chromatography with UV-detector refers to that the species of detection means is ultraviolet inspection
Survey device (UV detectors or referred to as UVD, and using the PDAD or referred to as DAD of ultraviolet detection principle)
HPLC.UV-detector is based on the detector that solute molecule absorbs the principle design of ultraviolet light, and its operation principle is Lambert-
Beer laws, i.e., when a monochromic beam passes through flow cell, if mobile phase does not absorb light, trap A is dense with extinction component
The optical path length L of degree C and flow cell is directly proportional compared to other types of detection means, and UV detectors have sensitivity higher,
The range of linearity is wide, and noise is low, and wavelength is optional, and the flow velocity and temperature change to mobile phase are insensitive, it is adaptable to gradient elution, to strong
Absorbing material detectable limit does not destroy sample up to below 10-9g/ml after detection.
The technology that above-mentioned standard curve method is well known to those skilled in the art.Calibration curve method calibration curve method is also referred to as
External standard method or direct comparison method, draw standard working curve with the standard sample of component to be surveyed first.Specifically the practice is：Use standard
Sample preparation into the standard series of various concentrations, under with component identical chromatographic condition to be surveyed, survey by isometric correct amount sample introduction
The peak area or peak height at each peak are measured, standard working curve, this standard working curve are drawn to sample concentration with peak area or peak height
Should be by the straight line of origin.If standard working curve by origin, does not illustrate that assay method has systematic error.Standard works
Slope of a curve is the Absolute Calibration factor.During constituent content in determination sample, with to draw standard working curve complete
Exactly the same chromatographic condition makes chromatogram, measures the peak area or peak height of chromatographic peak, then according to peak area and peak height in mark
The concentration into sample component in chromatographic column is directly found on quasi- working curve, also this can be calculated by formula (2-3-19) dense
Degree.
pi/ %=fiAi(hi) (2-3-19)
A in formulai(hi) --- the peak area (peak height) of i component peaks；
fi--- the slope of i Component Standard working curves.
Know into after the concentration of sample component in chromatographic column, so that it may calculate original according to sample processing conditions and sample size
The content of the component in sample.When constituent content to be surveyed change is little, and during the approximate content of known this component, it is also possible to
Standard working curve need not be drawn, and uses Single Point Correction Method, i.e., direct comparison method is quantified.First prepare one and constituent content to be measured
The standard liquid of close concentration known, under identical chromatographic condition, respectively by testing sample solution and standard sample solution
Isometric sample introduction, makes chromatogram, measures the peak area or peak height of component to be measured and standard sample, then straight by formula (2-3-20)
Connect the content for calculating component to be measured in sample solution：
W in formulas--- standard sample liquid quality fraction, %：
wi--- constituent mass fraction to be measured, % in sample solution：
As(hs) --- the peak area (peak height) of standard sample：
Ai(hi) --- the peak area (peak height) of i components in sample.
Single Point Correction Method be actually by the use of origin as standard working curve on another point.Therefore, when method is deposited
In systematic error (standard working curve does not pass through origin), the error of Single Point Correction Method is larger.The advantage of calibration curve method
It is：Work is determined after drawn standard working curve just very simple, can directly be read from standard working curve during calculating and contained
Amount, this is very suitable to a large amount of sample analysis.Particularly standard working curve can be using a period of time, at this section after drawing
It is interior single point correction to be carried out to standard working curve through commonly using a standard sample, to determine the standard working curve whether also
Can be used.
There is no particular limitation for the model and specification of the chromatographic column used as HPLC of the invention, for example its model and
Specification is 185 μm of 3.9 × 150mm of symmetryC.
Used as the mobile phase of the high performance liquid chromatography of invention, it can be 2~6 comprising volume ratio：94~98 it is organic molten
Agent and by mass ratio be 2.5~3.5：The cushioning liquid that 1 sodium dihydrogen phosphate, perfluorooctane sulfonate are constituted.Such as organic solvent
Can be 2 with the volume ratio of cushioning liquid：98、2.5：97.5、3：97、4：96、5：95、5.5：94.5、6：94 etc., preferably 4：
96。
Herein, organic solvent can include a kind of in methyl alcohol, acetonitrile or two kinds, more preferably acetonitrile, higher to obtain
Detection sensitivity.
The effect of cushioning liquid is the pH value for maintaining mobile phase, to cause that tested component keeps the stability of molecular state.
Sodium dihydrogen phosphate, the mass ratio of perfluorooctane sulfonate can be 2.5 in cushioning liquid：1、2.8：1、3：1、3.2：1、3.3：1、3.4：
1、3.5：1 etc., preferably 3：1.
The pH of above-mentioned mobile phase with 3.0~3.5, such as 3.0,3.2,3.5.Preferably 3.0, to keep reductive glutathione
High-absorbility.
The flow velocity of the chromatographic column of above-mentioned high performance liquid chromatography is 0.8~1.2mL/min, such as 0.8mL/min, 0.85mL/
Min, 0.90mL/min, 1.0mL/min, 1.1mL/min, 1.15mL/min or 1.2mL/min,；Preferably 1.0mL/min.
Used as high performance liquid chromatography of the invention, the temperature of its chromatographic column is advisable with 20~40 DEG C, preferably 30 DEG C.
The ultraviolet wavelength of above-mentioned UV-detector is preferred with 190~250nm, preferably 210nm.
It is the referring to property of concentration of the reference material of high performance liquid chromatography 150~250 μ g/ml, preferably 200 μ g/ml.
As mobile phase of the invention, deaerated using preceding ultrasonic wave, to avoid the bubble in mobile phase from increasing baseline
Noise, cause sensitivity to decline, or even cannot analyze.Meanwhile, some components that can also avoid the oxygen of dissolving also caused
It is oxidized, fixing phase occurs the separating property degraded and change post in post.
The present invention does not address part and is applied to prior art.
Unless otherwise defined, all technologies used herein and scientific terminology have and the common skill of art of the present invention
The identical implication that art personnel are generally understood that.When there is contradiction, the definition in this specification is defined.
Term as used herein：
" by ... prepare " it is synonymous with "comprising".Term "comprising" used herein, " including ", " having ", " containing "
Or its any other deformation, it is intended that cover including for non-exclusionism.For example, composition, step, method comprising listed elements,
Product or device are not necessarily limited to those key elements, and can be including not expressly listed other key elements or this kind of composition, step
Suddenly, method, product or the intrinsic key element of device.
Conjunction " by ... constitute " exclude any key element do not pointed out, step or component.If be used in claim,
This phrase will make claim be closed, it is not included the material in addition to the material that those are described, but relative
Except customary impurities.When phrase " by ... constitute " be rather than immediately following after theme in the clause that appears in claim main body
When, it is only limited to the key element described in the clause；Other key elements be not excluded as the overall claim it
Outward.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit
During the Range Representation that choosing value is limited, this is appreciated that and specifically discloses by any range limit or preferred value and any scope
All scopes that any pairing of lower limit or preferred value is formed, regardless of whether whether the scope separately discloses.For example, when open
During scope " 1~5 ", described scope should be interpreted as including scope " 1~4 ", " 1~3 ", " 1~2 ", " 1~2 and 4~
5 ", " 1~3 and 5 " etc..When number range is described herein, unless otherwise indicated, otherwise the scope is intended to include its end
Value and all integers and fraction within the range.
" mass parts " refer to the basic measurement unit of the mass ratio relation for representing multiple components, and 1 part can represent arbitrary list
Position quality, can such as be expressed as 1g, may also indicate that 2.689g etc..If we say that the mass parts of component A are a parts, the matter of B component
Amount part is b parts, then it represents that the quality of component A and the mass ratio a of B component：b.Or, the quality for representing component A is aK, B groups
The quality divided is bK (K is Arbitrary Digit, represents multiplying factor).Can not misread, and unlike mass fraction, all components
Mass parts sum be not limited to 100 parts of limitation.
"and/or" is used to represent that one of illustrated situation or both may to occur, for example, A and/or B includes (A
And B) and (A or B)；
Additionally, the indefinite article " one kind " and " one " before key element of the present invention or component are to key element or the quantitative requirement of component
(i.e. occurrence number) unrestriction.Therefore " one " or " one kind " should be read as including one or at least one, and odd number
The key element or component of form also include plural form, unless the obvious purport of the quantity refers to singulative.
Above-mentioned technical proposal of the invention has the beneficial effect that：
In such scheme, using the high performance liquid chromatography with UV-detector using in calibration curve method acquisition wheat embryo
The content of reductive glutathione content, so as to obtain measuring accuracy higher, may be adapted to reproducibility in Food and hygienical food
The detection of glutathione content.
Specific embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, below in conjunction with specific implementation
Example is described in detail.
Embodiment 1
Step one, according to following condition set chromatographic parameter：
Chromatogram column type number：symmetryC185μm 3.9×150mm；
Mobile phase：It is 2 comprising volume ratio：98 acetonitrile and by mass ratio be 2.5：1 sodium dihydrogen phosphate, perfluorooctane sulfonate
The cushioning liquid for being constituted；The pH of mobile phase is 3.0；
Chromatographic column：Flow velocity is 0.8mL/min；Temperature is 20 DEG C；Sample size is 10 μ l；
Ultraviolet light is detected：Wavelength is 190nm.
It is prepared by step 2, standard liquid：10.0mg glutathione reference substances accurately are weighed, high-purity water dissolves, and constant volume is used
To 50ml scales, concentration is 200 μ g/ml, is mixed standby.
Glutathione reference substance storing solution 0.0,0.5,2.5,5.0,7.5,12.5ml (200 μ g/ml) is taken respectively in 50ml
In volumetric flask, add water and be settled to scale mixing, equivalent to 0.0,2.0,10.0,20.0,30.0,50.0 μ g/ml, as standard system
Row apply liquid.
Step 3, sample preparation.After fluid sample is diluted or concentrates according to content height, 0.45 μm of filter membrane conduct is crossed
Sample analysis liquid.For solid sample, appropriate amount of sample (wheat embryo), plus distilled water are accurately weighed to 25ml scales, mixed, surpass
Sound wave extracts 5min, and centrifugation, supernatant crosses 0.45 μm of filter membrane as sample analysis liquid.
Step 4, Drawing of Curve.The sample analysis liquid and the μ l of standard control solution 10 for having prepared are taken respectively, by above-mentioned choosing
Fixed chromatographic condition, the standard liquid series application liquid that will be prepared is analyzed, and each concentration is repeated 3 times, with concentration as horizontal
Coordinate, peak area average is ordinate, draws standard working curve, and regression equation Y=5871C+ is tried to achieve by returning calculating
3949, correlation coefficient r=0.9999.
Embodiment 2
Step one, according to following condition set chromatographic parameter：
Chromatogram column type number：symmetryC185μm 3.9×150mm；
Mobile phase：It is 6 comprising volume ratio：94 methyl alcohol and by mass ratio be 3.5：1 sodium dihydrogen phosphate, perfluorooctane sulfonate
The cushioning liquid for being constituted；The pH of mobile phase is 3.2；
Chromatographic column：Flow velocity is 1.2mL/min；Temperature is 40 DEG C；Sample size is 10 μ l；
Ultraviolet light is detected：Wavelength is 200nm.
It is prepared by step 2, standard liquid：10.0mg glutathione reference substances accurately are weighed, high-purity water dissolves, and constant volume is used
To 50ml scales, concentration is 200 μ g/ml, is mixed standby.
Glutathione reference substance storing solution 0.0,0.5,2.5,5.0,7.5,12.5ml (200 μ g/ml) is taken respectively in 50ml
In volumetric flask, add water and be settled to scale mixing, equivalent to 0.0,2.0,10.0,20.0,30.0,50.0 μ g/ml, as standard system
Row apply liquid.
Step 3, sample preparation.After fluid sample is diluted or concentrates according to content height, 0.45 μm of filter membrane conduct is crossed
Sample analysis liquid.For solid sample, appropriate amount of sample (wheat embryo), plus distilled water are accurately weighed to 25ml scales, mixed, surpass
Sound wave extracts 5min, and centrifugation, supernatant crosses 0.45 μm of filter membrane as sample analysis liquid.
Step 4, Drawing of Curve.The sample analysis liquid and the μ l of standard control solution 10 for having prepared are taken respectively, by above-mentioned choosing
Fixed chromatographic condition, the standard liquid series application liquid that will be prepared is analyzed, and each concentration is repeated 3 times, with concentration as horizontal
Coordinate, peak area average is ordinate, draws standard working curve, and regression equation Y=5871C+ is tried to achieve by returning calculating
3949, correlation coefficient r=0.9999.
Embodiment 3
Step one, according to following condition set chromatographic parameter：
Chromatogram column type number：symmetryC185μm 3.9×150mm；
Mobile phase：It is 5 comprising volume ratio：95 methyl alcohol and by mass ratio be 3：1 sodium dihydrogen phosphate, perfluorooctane sulfonate institute
The cushioning liquid of composition；The pH of mobile phase is 3.5；
Chromatographic column：Flow velocity is 1mL/min；Temperature is 30 DEG C；Sample size is 10 μ l；
Ultraviolet light is detected：Wavelength is 250nm.
It is prepared by step 2, standard liquid：10.0mg glutathione reference substances accurately are weighed, high-purity water dissolves, and constant volume is used
To 50ml scales, concentration is 200 μ g/ml, is mixed standby.
Glutathione reference substance storing solution 0.0,0.5,2.5,5.0,7.5,12.5ml (200 μ g/ml) is taken respectively in 50ml
In volumetric flask, add water and be settled to scale mixing, equivalent to 0.0,2.0,10.0,20.0,30.0,50.0 μ g/ml, as standard system
Row apply liquid.
Step 3, sample preparation.After fluid sample is diluted or concentrates according to content height, 0.45 μm of filter membrane conduct is crossed
Sample analysis liquid.For solid sample, appropriate amount of sample (wheat embryo), plus distilled water are accurately weighed to 25ml scales, mixed, surpass
Sound wave extracts 5min, and centrifugation, supernatant crosses 0.45 μm of filter membrane as sample analysis liquid.
Step 4, Drawing of Curve.The sample analysis liquid and the μ l of standard control solution 10 for having prepared are taken respectively, by above-mentioned choosing
Fixed chromatographic condition, the standard liquid series application liquid that will be prepared is analyzed, and each concentration is repeated 3 times, with concentration as horizontal
Coordinate, peak area average is ordinate, draws standard working curve, and regression equation Y=5871C+ is tried to achieve by returning calculating
3949, correlation coefficient r=0.9999.
Embodiment 4
Step one, according to following condition set chromatographic parameter：
Chromatogram column type number：symmetryC185μm 3.9×150mm；
Mobile phase：It is 4 comprising volume ratio：96 acetonitrile and by mass ratio be 3：1 sodium dihydrogen phosphate, perfluorooctane sulfonate institute
The cushioning liquid of composition；The pH of mobile phase is 3.0；
Chromatographic column：Flow velocity is 1mL/min；Temperature is 30 DEG C；Sample size is 10 μ l；
Ultraviolet light is detected：Wavelength is 210nm.
It is prepared by step 2, standard liquid：10.0mg glutathione reference substances accurately are weighed, high-purity water dissolves, and constant volume is used
To 50ml scales, concentration is 200 μ g/ml, is mixed standby.
Glutathione reference substance storing solution 0.0,0.5,2.5,5.0,7.5,12.5ml (200 μ g/ml) is taken respectively in 50ml
In volumetric flask, add water and be settled to scale mixing, equivalent to 0.0,2.0,10.0,20.0,30.0,50.0 μ g/ml, as standard system
Row apply liquid.
Step 3, sample preparation.After fluid sample is diluted or concentrates according to content height, 0.45 μm of filter membrane conduct is crossed
Sample analysis liquid.For solid sample, appropriate amount of sample (wheat embryo), plus distilled water are accurately weighed to 25ml scales, mixed, surpass
Sound wave extracts 5min, and centrifugation, supernatant crosses 0.45 μm of filter membrane as sample analysis liquid.
Step 4, Drawing of Curve.The sample analysis liquid and the μ l of standard control solution 10 for having prepared are taken respectively, by above-mentioned choosing
Fixed chromatographic condition, the standard liquid series application liquid that will be prepared is analyzed, and each concentration is repeated 3 times, with concentration as horizontal
Coordinate, peak area average is ordinate, draws standard working curve, and regression equation Y=5871C+ is tried to achieve by returning calculating
3949, correlation coefficient r=0.9999.
Because the number range of each technological parameter involved in the present invention can not possibly all embody in the above-described embodiments,
As long as but those skilled in the art's envisioned any numerical value fallen into the above-mentioned number range completely can implement this
Invention, also includes any combination of occurrence in the range of some numerical value certainly.Herein, for the consideration of length, eliminate to
Go out the embodiment of occurrence in certain one or more number range, this disclosure for being not to be construed as technical scheme is not filled
Point.
Applicant states that the present invention illustrates detailed process equipment of the invention and technological process by above-described embodiment,
But the invention is not limited in above-mentioned detailed process equipment and technological process, that is, do not mean that the present invention has to rely on above-mentioned detailed
Process equipment and technological process could be implemented.Person of ordinary skill in the field it will be clearly understood that any improvement in the present invention,
Addition, the selection of concrete mode to the equivalence replacement and auxiliary element of each raw material of product of the present invention etc., all fall within of the invention
Within the scope of protection domain and disclosure.
Claims (8)
1. in a kind of wheat embryo reductive glutathione content detection method, it is characterised in that using band UV-detector
High performance liquid chromatography using calibration curve method obtain wheat embryo in reductive glutathione content content.
2. detection method according to claim 1, it is characterised in that the mobile phase of the high performance liquid chromatography includes volume
Than being 2~6：94~98 organic solvent and by mass ratio be 2.5~3.5：1 sodium dihydrogen phosphate, perfluorooctane sulfonate are constituted
Cushioning liquid.
3. detection method according to claim 1, it is characterised in that the organic solvent is methyl alcohol and/or acetonitrile, preferably
It is acetonitrile.
4. detection method according to claim 1, it is characterised in that the pH of the mobile phase is 3.0~3.5, preferably
3.0。
5. detection method according to claim 1, it is characterised in that the flow velocity of the chromatographic column of the high performance liquid chromatography is
0.8~1.2mL/min, preferably 1.0mL/min.
6. detection method according to claim 1, it is characterised in that the temperature of the chromatographic column of the high performance liquid chromatography is
20~40 DEG C.
7. detection method according to claim 1, it is characterised in that the ultraviolet light wave a length of 190 of the UV-detector
~250nm, preferably 210nm.
8. detection method according to claim 1, it is characterised in that use the reference material with the high performance liquid chromatography
Concentration is 150~250 μ g/ml, preferably 200 μ g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611107105.2A CN106770870A (en) | 2016-12-06 | 2016-12-06 | The detection method of reductive glutathione content in a kind of wheat embryo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611107105.2A CN106770870A (en) | 2016-12-06 | 2016-12-06 | The detection method of reductive glutathione content in a kind of wheat embryo |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106770870A true CN106770870A (en) | 2017-05-31 |
Family
ID=58878857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611107105.2A Pending CN106770870A (en) | 2016-12-06 | 2016-12-06 | The detection method of reductive glutathione content in a kind of wheat embryo |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106770870A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342610A (en) * | 2018-12-24 | 2019-02-15 | 无锡福祈制药有限公司 | The analysis method of glutathione content |
| CN111912926A (en) * | 2020-09-03 | 2020-11-10 | 克明面业股份有限公司 | Method for determining reduced glutathione content in rice by ultra-high performance liquid chromatography-tandem mass spectrometry |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827244A (en) * | 2012-08-20 | 2012-12-19 | 广东肇庆星湖生物科技股份有限公司 | Method for refining glutathione fermentation broth |
| CN105572234A (en) * | 2014-10-08 | 2016-05-11 | 江苏省中国科学院植物研究所 | Method for rapidly and efficiently determining cysteine content and glutathione content in iris lactea var. chinensis |
-
2016
- 2016-12-06 CN CN201611107105.2A patent/CN106770870A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827244A (en) * | 2012-08-20 | 2012-12-19 | 广东肇庆星湖生物科技股份有限公司 | Method for refining glutathione fermentation broth |
| CN105572234A (en) * | 2014-10-08 | 2016-05-11 | 江苏省中国科学院植物研究所 | Method for rapidly and efficiently determining cysteine content and glutathione content in iris lactea var. chinensis |
Non-Patent Citations (5)
| Title |
|---|
| 刘千 等: "麦胚谷胱甘肽提取与含量测定方法研究", 《中国粮油学报》 * |
| 方敬伟 等: "HPLC法测定不同厂家注射用还原型谷胱甘肽的含量", 《解放军药学学报》 * |
| 李敏: "HPLC法测定注射用硫酸头孢匹罗中还原型谷胱甘肽的含量", 《齐鲁药事》 * |
| 李鑫: "HPLC法检测酶法合成中还原型/氧化型谷胱甘肽", 《广东化工》 * |
| 王爱月 等: "高效液相色谱法测定保健食品中谷胱甘肽含量的方法研究", 《中国卫生检验杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342610A (en) * | 2018-12-24 | 2019-02-15 | 无锡福祈制药有限公司 | The analysis method of glutathione content |
| CN111912926A (en) * | 2020-09-03 | 2020-11-10 | 克明面业股份有限公司 | Method for determining reduced glutathione content in rice by ultra-high performance liquid chromatography-tandem mass spectrometry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Takahashi et al. | Quantitative comparison of a corona-charged aerosol detector and an evaporative light-scattering detector for the analysis of a synthetic polymer by supercritical fluid chromatography | |
| CN106770870A (en) | The detection method of reductive glutathione content in a kind of wheat embryo | |
| CN106198772A (en) | Measure the method for low free toluene diisocyanate content in base polyurethane prepolymer for use as | |
| CN109324135B (en) | Method for simultaneously detecting 3 emulsifiers in composite emulsifier | |
| Ceyhan et al. | LC determination of atropine sulfate and scopolamine hydrobromide in pharmaceuticals | |
| Desai et al. | High Performance Liquid Chromatography-A Validation View | |
| Murugan et al. | A review on method development and validation by using HPLC | |
| Zong et al. | Optimization and validation of an HPLC-photodiode array detector method for determination of organic acids in vinegar | |
| CN107271600A (en) | The detection method of isomer impurities content in a kind of 4 (3 fluorine benzyloxy) benzaldehydes | |
| CN105158348A (en) | Method for determining five effective components in zedoary oil by using gas chromatography | |
| CN102507776B (en) | Method for determining phenol content in water | |
| CN105301122A (en) | Method for determination of contents of oleanolic acid and ursolic acid in kiwi fruit oil through high performance liquid chromatography | |
| CN109298092A (en) | Detect the HPLC method of sulfonyloxy methyl chlorinity in industrial wastes | |
| Priya et al. | Method development and validation for quantification of propranolol HCl in pharmaceutical dosage form by RP-UPLC | |
| CN110441437A (en) | A kind of measuring method of Michaelis acid content | |
| CN106404948A (en) | POPS content analysis method | |
| Taleuzzaman et al. | Bio-Analytical Method Validation-A Review | |
| CN104897797A (en) | Method for determining oleanolic acid content and ursolic acid content in perilla frutescens oil through high performance liquid chromatography method | |
| Vuković et al. | One-step solid-phase UV spectrophotometric method for phenol determination in vaccines: Development and quality assessment | |
| Khan et al. | Development and validation of zero and first order spectrophotometric method for determination of opipramol in bulk and pharmaceutical dosage form | |
| WO2020261321A1 (en) | Analysis method | |
| Kumar et al. | A Review on Validation of RP-HPLC Method for the Simultaneous Estimation | |
| CN104897798A (en) | Method for determining oleanolic acid content and ursolic acid content in peanut oil through high performance liquid chromatography method | |
| Abdali et al. | Development of UV Spectrophotometric Methods for the Determination of Risedronate Sodium in Different Solutions | |
| Padmavathi et al. | Recent Development and Validation of RP-HPLC Method for Simultaneous Estimation of Grazoprevir and Elbasvir in Bulk and Pharmaceutical Dosage Form |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |