CN106770607A - A kind of method that utilization HS-IMR-MS differentiates genuine-fake cigarette - Google Patents
A kind of method that utilization HS-IMR-MS differentiates genuine-fake cigarette Download PDFInfo
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Abstract
The invention discloses a kind of method that utilization HS IMR MS differentiate genuine-fake cigarette, using head-space sampler and IMR MS Quick Acquisition cigarette volatile ingredient and be analyzed, obtain the volatile ingredient mass spectrum fingerprint pattern of genuine piece cigarette and same trade mark cigarette to be measured, with reference to similarity analysis, the contrast qualified threshold value of genuine piece cigarette, realizes the quick discriminating to genuine-fake cigarette to be measured.The beneficial effects of the invention are as follows：Head-space sampler is used in series with ion molecule reaction mass spectrograph, become a kind of analysis of volatile components technology for integrating sample collection, sample introduction, analysis, with reference to mass spectrometric data similarity analysis, a kind of easy to operate, objective and fair, the accurate rapid analysis method of science can be provided for genuine-fake cigarette differentiates, fundamentally improve the scientific and authority of genuine-fake cigarette diagnostic test work, to resist the personation cigarette for being all the more difficult to differentiate, country and consumer's interests are safeguarded.
Description
Technical field
It is particularly a kind of to utilize HS-IMR-MS (head spaces-ion molecule reaction the present invention relates to cigarette identification technology field
Mass spectrum) differentiate genuine-fake cigarette method.
Background technology
China is a production of cigarettes big country, and cigarette output is high, and the production trade mark is more.Although country adopts various measures, no
It is disconnected to strengthen tobacco monopoly administration, but still have some lawless persons to seek exorbitant profit, manufactured using various means and sale personation
Cigarette, and, with the high development of modern science and technology, the technology of its false making and counterfeit is also increasingly superb.Now, personation volume
The producer of cigarette uses more advanced volume bag equipment, there is the technical recipe of oneself, rolls crudy with " genuine products " without bright
Not, cause false smoke and true cigarette is increasingly difficult to differentiate between significant difference in external packing, has reached the degree mixed the spurious with the genuine, and consumer is past
It is past to have dust thrown into the eyes.Substantial amounts of personation cigarette comes into the market, and very disruptive market economy compromises country, business and consumer
Interests.
《Cigarette product diagnostic test management method》Clear stipulaties, the method for genuine-fake cigarette diagnostic test mainly has three kinds：
Sense organ differential method, smoke panel test differential method and Instrumental Analysis differential method.Wherein, the sense organ differential method for being aided with the method for smokeing panel test is the current true and false
The discrimination method commonly used in discrimination process.However, this for standards such as sample raw material, production technology feature, anti-counterfeiting technologies
Based on discrimination method, the authentication technique of related appraiser is depended on higher to a certain extent, for emulator
For cigarette product higher, adopt this method and realize that the true and false of cigarette differentiates there is certain limitation.
Differentiate the limitation for existing based on sense organ, with the development of instrument analysis technology, genuine-fake cigarette authentication technique is gradually
It is combined with instrument analysis technology.Sense organ authentication technique is different from, instrument analysis technology has broken away from the subjective factor of identification person, should
With accurate instrument, by data processing, the discriminating to genuine-fake cigarette is realized.At present, in genuine-fake cigarette discriminating, using most
Instrument analysis technology be Electronic Nose Technology and near-infrared spectrum technique, SPME-gas chromatography-mass spectrum technology, head space-
Gas chromatography-mass spectrum technology etc. is also appeared in the newspapers.
However, the measure of the index of correlation of instrument diagnostic test is generally required carries out pre-treatment, minute is more long, and cigarette
The complex chemical composition of grass itself, causes the reduction of the mathematical modeling degree of accuracy, constrains related application.
Thus for subjective factor present in existing genuine-fake cigarette discrimination method substantially, complex pretreatment, analysis time
Long the problems such as, it is necessary to utilize new instrument analytical technology, research and develop more objective science genuine-fake cigarette authentication technique and
Method.
The content of the invention
It is an object of the invention to overcome genuine-fake cigarette to differentiate the shortcoming of prior art, there is provided a kind of easy to operate, objective
The method that just, science is accurate, differentiate genuine-fake cigarette using HS-IMR-MS.
The purpose of the present invention is achieved through the following technical solutions：A kind of utilization HS-IMR-MS differentiates the side of genuine-fake cigarette
Method, using head-space sampler and IMR-MS (ion molecule reaction mass spectrum) Quick Acquisition cigarette volatile ingredient and divided
Analysis, obtains the volatile ingredient mass spectrum fingerprint pattern of genuine piece cigarette and same trade mark cigarette to be measured, with reference to similarity analysis, contrast
The qualified threshold value of genuine piece cigarette, realizes the quick discriminating to genuine-fake cigarette to be measured.
The method that a kind of described utilization HS-IMR-MS differentiates genuine-fake cigarette, comprises the following steps：
The preparation of S1, sample；
Prepare at least 10 each one of the same trade mark genuine piece cigarette of batch, randomly selecting 1 cigarette for every carries out reality
Test；Prepare the cigarette to be identified of the correspondence trade mark in addition, randomly select 3 as cigarette to be measured；
Above-mentioned genuine piece cigarette and cigarette to be measured are placed in the 20mL ml headspace bottles of sealing respectively, in order by blank
Bottle, genuine piece cigarette bottle and cigarette to be measured bottle are positioned on head-space sampler sample injection disc, and in each two sample room insertion blank top
Empty bottle, for being spaced suction, removes pipeline residual；
S2, HS-IMR-MS instrumental conditions are set：
S21, headspace autosampler analysis condition are as follows：
Sample equilibration temperature：50 DEG C -150 DEG C, quantitative loop temperature：50 DEG C -150 DEG C, transmission line temperature：50℃‐150℃；
Quantitative loop volume：1mL；Sample equilibration time：2min‐30min；Pressing time：0.2min；Loop fills the time：
0.2min；The loop balance time：0.05min；Sample injection time：1min；
S22, IMR-MS analysis condition are as follows：
Open IMR-MS systems and preheat more than 48 hours, Single Masses methods are set in IMR-MS work stations,
And following parameter setting is carried out in the method：
It is each scanning ion of 16-300 that mass number is added in ion inventory is scanned, and sets every kind of ion：During scanning
Between：50ms‐300ms；MS acquisition pressure：35mbar；Ionization reagents：Xe, reagent ionization energy：12.13eV, or ionization reagents：
Kr, reagent ionization energy：14.00eV；Resolution ratio：1amu；Cycles samples scanning times：5 times；
The average mass spectrum fingerprint pattern of S3, genuine piece cigarette is obtained；
Each five average values of scanning result of ion of genuine piece cigarette are calculated, and it is average to deduct correspondence ion in blank
Value, obtains final product the response (cps) of each ion of genuine piece cigarette.Mapped with the response of each ion and respective quality number, you can obtain
The mass spectrum fingerprint pattern of genuine piece cigarette；By multiple batches of (>=10 batches) genuine piece cigarette retest, the flat of the genuine piece cigarette is determined
Equal mass spectrum fingerprint pattern；The mass number situation, matter contained by genuine piece cigarette can intuitively be understood by average mass spectrum fingerprint pattern
The response of each ion is strong at the mass number from the molecular ion or fragment ion of different compounds in spectrogram
Degree sum；
S4, determine the qualified threshold value of genuine piece cigarette；
The mass spectrum fingerprint pattern of each genuine piece cigarette and average mass spectrum fingerprint pattern data are imported into SPSS statistical analyses soft
Part, using Bivariate analysis, each genuine piece cigarette mass spectrum fingerprint pattern and average matter is calculated using Pearson correlation coefficient method
Similarity between spectrum finger-print, the qualified threshold value of the trade mark genuine piece cigarette is determined according to wherein minimum similarity, and definition is closed
Lattice threshold value is less than minimum similarity 3%；
S5, cigarette to be measured identification；
According to step S3, the mass spectrum fingerprint pattern of cigarette to be measured is obtained, calculate cigarette mass spectrum fingerprint pattern peace to be measured
The similarity of equal mass spectrum fingerprint pattern, compares qualified threshold value, judges that such as similarity value calculation is less than to genuine-fake cigarette to be measured
Qualified threshold value, then can determine that to be personation cigarette.
In described step S21, Sample equilibration temperature：80 DEG C, quantitative loop temperature：100 DEG C, transmission line temperature：120℃.
In described step S21, Sample equilibration time is 5min.
In described step S22, sweep time is 300ms.
In described step S22, it is each scanning ion of 16-200 that mass number is added in ion inventory is scanned.
Described use Pearson correlation coefficient method calculates each genuine piece cigarette mass spectrum fingerprint pattern and average mass spectrum fingerprint image
The computational methods of the similarity between spectrum are as shown in Equation 1：
R in formula (1)xyThat is the coefficient correlation of two cigarette collection of illustrative plates, x and y are represented and are carried out two cigarette mass spectrums of similarity-rough set and refer to
Line collection of illustrative plates, wherein xiAnd yiThe response of i-th data point of x and y is represented,WithRepresent the average of x and y mass spectrometric datas.R is retouched
What is stated is the strong and weak degree of linear correlation between two cigarette collection of illustrative plates, and its absolute value is bigger and closer to the similar of 1, two collection of illustrative plates
Degree is higher, i.e., two cigarette samples are more consistent；Its absolute value is smaller and closer to 0, and two collection of illustrative plates similarity degrees are smaller, i.e., and two
Cigarette sample is more inconsistent.
The application field of this method includes that true and false cigarette differentiates, the lot stability inspection of same cigarette production line and difference are rolled up
The uniform quality sex determination of cigarette production line.
Ion molecule reaction mass spectrum (IMR-MS) has been widely used in breathing as a kind of new soft ionization mass-spectrometric technique
Qi leel is analysed, analysing tail gas of vehicle and ambient air pollutant analysis etc., fast with analyze speed, without advantages such as pre-treatments.
In the present invention, head-space sampler is connected with IMR-MS, becomes and a kind of integrate sample collection, sample introduction, analysis
Analysis of volatile components technology, with reference to mass spectrometric data similarity analysis, so that it may for genuine-fake cigarette differentiate provides one kind it is easy to operate,
The accurate rapid analysis method of objective and fair, science.
The present invention has advantages below：
1st, it is easy to operate without carrying out any pre-treatment to cigarette from headspace sampling.
2nd, it is analyzed using IMR-MS, the measure to cigarette, analyze speed can be completed within a few minutes even several seconds
Hurry up.
3rd, the subjective factor of identification person has been broken away from, whole discrimination process, as foundation, is realized more with the data of objective science
Equitably judge genuine-fake cigarette.
4th, HS-IMR-MS carries out identification using qualified threshold value, and decision method is simple and easy to apply, analysis process repeatability
Good, it is accurate as a result to stablize, and can make and sell fake and forged cigarette product illegal activity for strike and provide scientific basis.
Brief description of the drawings
Fig. 1 is genuine piece cloud and mist (soft treasure) average mass spectrum fingerprint pattern of cigarette in the embodiment of the present invention 1.
Fig. 2 is genuine piece Yuxi (soft) average mass spectrum fingerprint pattern of cigarette in the embodiment of the present invention 2.
Fig. 3 is genuine piece Hongta (hard classical) average mass spectrum fingerprint pattern of cigarette in the embodiment of the present invention 3.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention will be further described：
Embodiment 1
A kind of method that utilization HS-IMR-MS differentiates genuine-fake cigarette, is rolled up using head-space sampler and IMR-MS Quick Acquisitions
The volatile ingredient of cigarette is simultaneously analyzed, and obtains the volatile ingredient mass spectrum fingerprint image of genuine piece cigarette and same trade mark cigarette to be measured
Spectrum, with reference to similarity analysis, contrasts the qualified threshold value of genuine piece cigarette, realizes the quick discriminating to genuine-fake cigarette to be measured.
Specifically include following steps：
The preparation of S1, sample,
Prepare 10 each one of batch genuine piece cloud and mist (soft treasure) cigarette of in March, 2016-July production, every each random
1 cigarette is extracted to be tested；Prepare personation cloud and mist (soft treasure) cigarette one being collected into addition, randomly select 3 conducts
Cigarette to be measured.
Above-mentioned 10 genuine piece clouds and mists (soft treasure) and 3 clouds and mists (soft treasure) to be measured are placed in the 20mL head spaces of sealing respectively
In bottle, blank bottle, genuine piece bottle and sample bottle to be tested are positioned on head-space sampler sample injection disc in order, and in every two
Individual sample room inserts blank ml headspace bottle.
S2, HS-IMR-MS instrumental conditions are set：
S21, headspace autosampler analysis condition are as follows：
Sample equilibration temperature：80 DEG C, quantitative loop temperature：100 DEG C, transmission line temperature：120℃；In, three above temperature
Degree can as needed in 50 DEG C of -150 DEG C of scopes selections；
Quantitative loop volume：1mL；Sample equilibration time：5min, in, can as needed in the range of 2min-30min
Selection；Pressing time：0.2min；Loop fills the time：0.2min；The loop balance time：0.05min；Sample injection time：1min.
S22, IMR-MS analysis condition are as follows：
Open IMR-MS systems and preheat more than 48 hours, Single Masses methods are set in IMR-MS work stations,
And following parameter setting is carried out in the method：
It is each scanning ion of 16-200 that mass number is added in ion inventory is scanned, and in, can be existed as needed
Scanning Section is selected in the range of 16-300 mass numbers；
Every kind of ion is set：Sweep time：300ms, in, can select in the range of 50ms-300ms as needed；
MS acquisition pressure：35mbar；Ionization reagents：Xe, reagent ionization energy：12.13eV, also dependent on the selection ionization examination of the demand of measure
Agent：Kr, reagent ionization energy：14.00eV；Resolution ratio：1amu；Cycles samples scanning times：5 times.
The average mass spectrum fingerprint pattern of S3, genuine piece cigarette is obtained
Calculate genuine piece cloud and mist (soft treasure) five average values of scanning result of each ion, and deduct in blank correspondence from
Sub- average value, obtains final product the response of genuine piece cloud and mist (soft treasure) each ion.Mapped with the response of ion and respective quality number, obtained
To the mass spectrum fingerprint pattern of genuine piece cloud and mist (soft treasure).By the retest of multiple batches of (10 batches) genuine piece cloud and mist (soft treasure), really
Determine the average mass spectrum fingerprint pattern of genuine piece cloud and mist (soft treasure), as shown in Figure 1.
S4, determine the qualified threshold value of genuine piece cigarette
The mass spectrum fingerprint pattern of 10 batches of genuine piece clouds and mists (soft treasure) and average mass spectrum fingerprint pattern data are imported into SPSS
19.0 statistical analysis softwares, using Bivariate analysis, calculate each genuine piece cloud and mist (soft treasure) mass spectrum fingerprint pattern with it is average
Similarity between mass spectrum fingerprint pattern, and the qualified threshold value of genuine piece cloud and mist (soft treasure) is determined according to wherein minimum similarity.
Similarity Measure result is as shown in table 1.As shown in Table 1, genuine piece cloud and mist (soft treasure) mass spectrum fingerprint pattern and average mass spectrum fingerprint
The minimum similarity of collection of illustrative plates is 98.12%, therefore the minimum qualified threshold value of determination genuine piece cloud and mist (soft treasure) is 95.2%.
The similarity of the genuine piece cloud and mist of table 1 (soft treasure) mass spectrum fingerprint pattern and average mass spectrum fingerprint pattern
S5, cigarette to be measured identification
According to step S3, the mass spectrum fingerprint pattern of cloud and mist to be measured (soft treasure) is obtained, calculate cloud and mist to be measured (soft treasure)
The similarity of mass spectrum fingerprint pattern peace mass spectrum fingerprint pattern, compares the qualified threshold value of genuine piece cigarette, and genuine-fake cigarette to be measured is entered
Row is evaluated, and evaluation result is as shown in table 2.As known from Table 2,3 for thus being randomly selected in bar cloud and mist to be identified (soft treasure)
The mass spectrum fingerprint pattern of cigarette is respectively 92.21%, 93.50%, 93.14% with the similarity of average mass spectrum fingerprint pattern,
Less than genuine piece cloud and mist (soft treasure) qualified threshold value 95.2%, it is judged to cloud and mist (soft treasure) cigarette of personation.The result and sense organ
The identification result that differentiates and smoke panel test is consistent.
Table 2 cloud and mist to be measured (soft treasure) genuine-fake cigarette result of determination
| Cigarette sample number to be measured | Similarity (%) | Minimum qualified threshold value (%) | Result of determination |
| 1 | 92.21 | 95.2 | Adulterant |
| 2 | 93.50 | 95.2 | Adulterant |
| 3 | 93.14 | 95.2 | Adulterant |
The application field of this method includes that true and false cigarette differentiates, the lot stability inspection of same cigarette production line and difference are rolled up
The uniform quality sex determination of cigarette production line.
To investigate applicability of the present invention to other trade mark cigarette, embodiment 2~3 is carried out according to embodiment 1, and following
Aspect is adjusted accordingly：
Embodiment 2
In embodiment 2,10 batch genuine piece Yuxi (soft) cigarette each, every of in April, 2016-July production are chosen
1 cigarette is respectively randomly selected to be tested；Prepare personation Yuxi (soft) cigarette one being collected into addition, randomly select 3 flags
It is cigarette to be measured, remaining is implemented according to embodiment 1.
The average mass spectrum fingerprint pattern in genuine piece Yuxi (soft) as shown in Fig. 2 10 batches of genuine piece Yuxi (soft) mass spectrum fingerprint patterns with
As shown in table 3, cigarette result of determination to be measured is as shown in table 4 for similarity between average mass spectrum fingerprint pattern.
As shown in Table 3, genuine piece Yuxi (soft) and the minimum similarity of average mass spectrum fingerprint pattern are 97.70%, therefore are determined
The qualified threshold value in genuine piece Yuxi (soft) is 94.8%.As known from Table 4, by randomly selected in this Yuxi to be identified (soft) 3
The mass spectrum fingerprint pattern of branch cigarette is respectively 89.81%, 90.78%, 91.26% with the similarity of average mass spectrum fingerprint pattern,
Respectively less than genuine piece Yuxi (soft) qualified threshold value 94.8%, therefore it is judged to Yuxi (soft) cigarette of personation.This result differentiates with sense organ
And identification result of smokeing panel test is consistent.
The similarity of genuine piece Yuxi (soft) mass spectrum fingerprint pattern of table 3 and average mass spectrum fingerprint pattern
Table 4 Yuxi to be measured (soft) genuine-fake cigarette result of determination
| Cigarette sample number to be measured | Similarity (%) | Minimum qualified threshold value (%) | Result of determination |
| 1 | 89.81 | 94.8 | Adulterant |
| 2 | 90.78 | 94.8 | Adulterant |
| 3 | 91.26 | 94.8 | Adulterant |
Embodiment 3
In embodiment 3,10 batch genuine piece Hongtas (hard classical) cigarette each of the production of 2 months-June in 2016 is chosen
Bar, randomly selects 1 cigarette for every and is tested；Prepare Hongta to be identified (hard classical) cigarette collected on the market in addition
, 3 are randomly selected as cigarette to be measured, remaining is implemented according to embodiment 1 by one.
Genuine piece Hongta (hard classical) average mass spectrum fingerprint pattern is as shown in figure 3,10 batches of genuine piece Hongta (hard classical) matter
As shown in table 5, cigarette result of determination to be measured is as shown in table 6 for similarity between spectrum finger-print and average mass spectrum fingerprint pattern.
As shown in Table 5, genuine piece Hongta (hard classical) and the minimum similarity of average mass spectrum fingerprint pattern are 97.12%,
Therefore the qualified threshold value of determination genuine piece Hongta (hard classical) is 94.2%.As known from Table 6, it is (hard by this Hongta to be identified
It is classical) in 3 mass spectrum fingerprint patterns of cigarette randomly selecting be respectively with the similarity of average mass spectrum fingerprint pattern
98.86%, 99.01%, 98.88%, qualified threshold value 94.2% is all higher than, therefore be judged to genuine piece Hongta (hard classical) cigarette.
The result is consistent with sense organ identification result.
The similarity of the genuine piece Hongta of table 5 (hard classical) mass spectrum fingerprint pattern and average mass spectrum fingerprint pattern
The Hongta to be measured of table 6 (hard classical) genuine-fake cigarette result of determination
| Cigarette sample number to be measured | Similarity (%) | Minimum qualified threshold value (%) | Result of determination |
| 1 | 98.86 | 94.2 | Genuine piece |
| 2 | 99.01 | 94.2 | Genuine piece |
| 3 | 98.88 | 94.2 | Genuine piece |
Claims (7)
1. a kind of method that utilization HS-IMR-MS differentiates genuine-fake cigarette, it is characterised in that：It is fast with IMR-MS using head-space sampler
The volatile ingredient of speed collection cigarette is simultaneously analyzed, and obtains the volatile ingredient matter of genuine piece cigarette and same trade mark cigarette to be measured
Spectrum finger-print, with reference to similarity analysis, contrasts the qualified threshold value of genuine piece cigarette, realizes the quick discriminating to genuine-fake cigarette to be measured.
2. the method that a kind of utilization HS-IMR-MS according to claim 1 differentiates genuine-fake cigarette, it is characterised in that：Including
Following steps：
The preparation of S1, sample；
Prepare at least 10 each one of the same trade mark genuine piece cigarette of batch, randomly select 1 cigarette for every and tested；Separately
The outer cigarette to be identified for preparing the correspondence trade mark, randomly selects 3 as cigarette to be measured；
Above-mentioned genuine piece cigarette and cigarette to be measured are placed in the 20mL ml headspace bottles of sealing respectively, in order by blank bottle, true
Product cigarette bottle and cigarette to be measured bottle are positioned on head-space sampler sample injection disc, and insert blank ml headspace bottle in each two sample room,
For being spaced suction, pipeline residual is removed；
S2, HS-IMR-MS instrumental conditions are set：
S21, headspace autosampler analysis condition are as follows：
Sample equilibration temperature：50 DEG C -150 DEG C, quantitative loop temperature：50 DEG C -150 DEG C, transmission line temperature：50℃‐150℃；
Quantitative loop volume：1mL；Sample equilibration time：2min‐30min；Pressing time：0.2min；Loop fills the time：
0.2min；The loop balance time：0.05min；Sample injection time：1min；
S22, IMR-MS analysis condition are as follows：
Open IMR-MS systems and preheat more than 48 hours, the setting Single Masses methods in IMR-MS work stations, and
Following parameter setting is carried out in the method：
It is each scanning ion of 16-300 that mass number is added in ion inventory is scanned, and sets every kind of ion：Sweep time：
50ms‐300ms；MS acquisition pressure：35mbar；Ionization reagents：Xe, reagent ionization energy：12.13eV, or ionization reagents：Kr,
Reagent ionization energy：14.00eV；Resolution ratio：1amu；Cycles samples scanning times：5 times；
The average mass spectrum fingerprint pattern of S3, genuine piece cigarette is obtained；
Each five average values of scanning result of ion of genuine piece cigarette are calculated, and deducts correspondence ion average value in blank, i.e.,
Obtain the response of each ion of genuine piece cigarette；Mapped with the response of each ion and respective quality number, you can obtain genuine piece cigarette
Mass spectrum fingerprint pattern；By no less than 10 batch genuine piece cigarette retests, determining the average mass spectrum fingerprint image of the genuine piece cigarette
Spectrum；
S4, determine the qualified threshold value of genuine piece cigarette；
The mass spectrum fingerprint pattern of each genuine piece cigarette and average mass spectrum fingerprint pattern data are imported into SPSS statistical analysis softwares, profit
With Bivariate analysis, each genuine piece cigarette mass spectrum fingerprint pattern and average mass spectrum fingerprint are calculated using Pearson correlation coefficient method
Similarity between collection of illustrative plates, the qualified threshold value of the trade mark genuine piece cigarette is determined according to wherein minimum similarity, defines qualified threshold value
It is less than minimum similarity 3%；
S5, cigarette to be measured identification；
According to step S3, the mass spectrum fingerprint pattern of cigarette to be measured is obtained, calculate cigarette mass spectrum fingerprint pattern peace homogeneous to be measured
The similarity of finger-print is composed, qualified threshold value is compareed, genuine-fake cigarette to be measured is judged, such as similarity value calculation is less than qualified
Threshold value, then can determine that to be personation cigarette.
3. the method that a kind of utilization HS-IMR-MS according to claim 2 differentiates genuine-fake cigarette, it is characterised in that：It is described
The step of S21 in, Sample equilibration temperature：80 DEG C, quantitative loop temperature：100 DEG C, transmission line temperature：120℃.
4. the method that a kind of utilization HS-IMR-MS according to claim 2 differentiates genuine-fake cigarette, it is characterised in that：It is described
The step of S21 in, Sample equilibration time is 5min.
5. the method that a kind of utilization HS-IMR-MS according to claim 2 differentiates genuine-fake cigarette, it is characterised in that：It is described
The step of S22 in, sweep time is 300ms.
6. the method that a kind of utilization HS-IMR-MS according to claim 2 differentiates genuine-fake cigarette, it is characterised in that：It is described
In step S22, it is each scanning ion of 16-200 that mass number is added in ion inventory is scanned.
7. the method that a kind of utilization HS-IMR-MS according to claim 1 differentiates genuine-fake cigarette, it is characterised in that：It is commented
Valency applicable object includes the discriminating of true and false cigarette, the lot stability inspection of same cigarette production line and the quality of kinds of cigarettes production line
Consistency checking.
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| CN109655529A (en) * | 2017-10-12 | 2019-04-19 | 贵州中烟工业有限责任公司 | A method of identifying genuine-fake cigarette |
| CN109738569A (en) * | 2019-02-28 | 2019-05-10 | 云南中烟工业有限责任公司 | A kind of imitative identification of cigarettes method of the height based on SPME-GC-MS |
| CN110763794A (en) * | 2019-11-12 | 2020-02-07 | 云南中烟工业有限责任公司 | Method for measuring volatile organic compounds in tobacco material |
| CN113655110A (en) * | 2021-07-28 | 2021-11-16 | 中国科学院合肥物质科学研究院 | Mass spectrum detection method and device for rapidly discriminating different brands of cigarettes on line |
| CN118094467A (en) * | 2024-02-19 | 2024-05-28 | 江苏朗沁科技有限公司 | Method for identifying authenticity of source tracing production place of functional skin care product |
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| CN109738569A (en) * | 2019-02-28 | 2019-05-10 | 云南中烟工业有限责任公司 | A kind of imitative identification of cigarettes method of the height based on SPME-GC-MS |
| CN109738569B (en) * | 2019-02-28 | 2022-02-01 | 云南中烟工业有限责任公司 | High-imitation cigarette identification method based on SPME-GC-MS |
| CN110763794A (en) * | 2019-11-12 | 2020-02-07 | 云南中烟工业有限责任公司 | Method for measuring volatile organic compounds in tobacco material |
| CN113655110A (en) * | 2021-07-28 | 2021-11-16 | 中国科学院合肥物质科学研究院 | Mass spectrum detection method and device for rapidly discriminating different brands of cigarettes on line |
| CN113655110B (en) * | 2021-07-28 | 2024-08-02 | 中国科学院合肥物质科学研究院 | Mass spectrum detection method and device for rapidly screening cigarettes of different brands on line |
| CN118094467A (en) * | 2024-02-19 | 2024-05-28 | 江苏朗沁科技有限公司 | Method for identifying authenticity of source tracing production place of functional skin care product |
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