CN106770251A - A kind of urinalysis test paper and preparation method thereof - Google Patents
A kind of urinalysis test paper and preparation method thereof Download PDFInfo
- Publication number
- CN106770251A CN106770251A CN201611252827.7A CN201611252827A CN106770251A CN 106770251 A CN106770251 A CN 106770251A CN 201611252827 A CN201611252827 A CN 201611252827A CN 106770251 A CN106770251 A CN 106770251A
- Authority
- CN
- China
- Prior art keywords
- sample
- detection block
- prepared
- detection
- completely dissolved
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The present invention is a kind of urinalysis test paper and preparation method thereof, test paper includes multiple detection blocks and multiple spacing block compositions, imbibition area is made up of absorbent material, spacing block is made up of hydrophobic material, there is the chemical reagent for detecting projects in detection block, substrate is padded on test paper lower section, and substrate center is provided with penetrating sample holes.The invention provides a kind of urinalysis test paper and preparation method thereof, each detection block is spaced by hydrophobic barrier, urine is dropped to by each detection block by open-work, make the reagent in urine and each detection block while reacting, multiple test modules are integrated on same miniature test paper, not using only amount of urine it is few, and whole detection process can complete in moment, without the careful dropwise addition urine successively as conventional Urine test paper bar, testing cost can be greatly reduced while raising detection efficiency.
Description
Technical field
The present invention relates to in-vitro diagnosis field, more particularly to a kind of urinalysis test paper and preparation method thereof.
Background technology
Dry analysis technology refer to fluid test sample is applied directly to it is commercialized dry for the specific production of disparity items
On dry reagent strip, specific chemical reaction is caused as solvent using the moisture of sample, be with enzyme so as to carry out chemical analysis
An alanysis method based on method, is widely used in urine detection and analysis, by the phase in various professional test paper and urine
Answer composition to chemically react, make the color of test paper change to determine whether urine contains certain composition or component content.
In existing test strips can integrated various detection projects reagent so that test strips include multiple item detection blocks, but
Existing test strips volume is larger, and generally long narrow strip in the block of particular detection project, it is necessary to drip by several times, accurately
Plus urine to be picked up, the urine sample amount for not only needing is larger, and detection efficiency is relatively low.
The content of the invention
Present invention seek to address that the deficiencies in the prior art, and a kind of urinalysis test paper and preparation method thereof is provided.
The present invention to achieve the above object, using following technical scheme：A kind of urinalysis test paper, it is characterised in that bag
Include test paper and substrate, the test paper includes imbibition area and the detection zone of multiple rectangular strips, multiple imbibition areas with it is many
The individual detection zone is spaced, and each detection zone is made up of multiple detection blocks and multiple spacing blocks, multiple spacing blocks and
Multiple detection blocks are spaced,
The imbibition area is made up of absorbent material, and the spacing block is made up of hydrophobic material,
The substrate is padded on the test paper lower section, and the substrate center is provided with penetrating sample holes.
Particularly, the sample holes have three, and three sample holes are corresponded respectively at the different imbibition areas.
Particularly, the substrate edge is provided with baffle plate upward, and the test paper is fixed on the base by the baffle plate
On plate.
Particularly, it is in blotting paper, glass fibre membrane and polyester fiber film to prepare the absorbent material in the imbibition area
Kind；It is the one kind in curable polymer and photoresist to prepare the hydrophobic material of the spacing block.
The present invention also provides a kind of preparation method of urinalysis test paper, it is characterised in that its step is as follows：
1. absorbent material is cut to size 10mm × 7mm as test paper base material, is dripped using hydrophobic material and is dipped into test paper base
On material and it is allowed to polymerizing curable and is formed act as the spacing block of hydrophobic barrier, test paper base material is separated into multiple detection blocks, makes every
The surrounding of individual detection block is surrounded by hydrophobic barrier；
2. chemical reagent dropwise addition and treatment are carried out in multiple detection blocks respectively, multiple detection blocks is prepared into respectively and is used to
Detect the item detection block of disparity items；
Described item detection block is selected from glucose detection block, acid-base value detection block, protein detection block, microalbumin
Detection block, proportion detection block, ketoboidies detection block, bilirubin detection block, UBG detection block, nitrite detection block, leucocyte
Detection block, ascorbic acid detection block, occult blood detection block, uric acid detection block, creatinine detection block, calcium detection block, projects detection block
Preparation method be specially：
It is prepared by glucose detection block
Weigh 0.19g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena adjusts solution PH to 6.0, takes 0.025g tetramethyl benzidines, and 920u glucose oxidases, 19u peroxidase is added
To in above-mentioned cushioning liquid, stirring is to being completely dissolved；Solution will be prepared to glucose detection using pipettor or auto sample applicator
Region carries out point sample, and sample size is 0.5~1 μ l, 25~35 DEG C of drying after the completion of point sample；
It is prepared by acid-base value detection block
1.2g methyl reds are weighed, 0.8g bromothymol blues, 0.8ml ethanol is added into the ultra-pure water of 10ml, fully stirred
Mix to being completely dissolved；Point sample, sample size are carried out to acid-base value detection zone by solution is prepared using pipettor or auto sample applicator
It is 0.5~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by protein detection block
Weigh 0.095g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L
NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.8ml ethanol, 0.025g polyvinyl alcohol, 0.033g to 2.0~3.0
Tetrabromophenol Blue is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution will be prepared using pipettor or auto sample applicator
Point sample is carried out to protein detection region, sample size is 0.5~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by microalbumin detection block
Weigh 1.9g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and 1mol/L hydrochloric acid conditioning solutions PH to 2.0~3.0, take 0.01g sulfonephthalein dyestuffs and add into above-mentioned cushioning liquid, stir
To being completely dissolved；Point sample, sample are carried out to microalbumin detection zone by solution is prepared using pipettor or auto sample applicator
It is 0.5~1 μ l to measure, 50~70 DEG C of drying after the completion of point sample；
It is prepared by proportion detection block
The metaphosphoric acids of 0.012g tri- are weighed, 0.018g bromothymol blues and 0.05g tetramethylammonium hydroxides add surpassing to 10ml
In pure water, it is stirred well to and is completely dissolved, will prepare solution contrast re-detection region using pipettor or auto sample applicator is carried out
Point sample, sample size is 0.5~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by ketoboidies detection block
0.5g NaOH is weighed, 0.125g sodium nitroprussides are added into the ultra-pure water of 10ml, are stirred well to
It is completely dissolved, will prepare solution using pipettor or auto sample applicator carries out point sample to ketoboidies detection zone, sample size is 0.5~
1 μ l, 35~50 DEG C of drying after the completion of point sample；
It is prepared by bilirubin detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take the dichloro diazols of 0.35g 2,6 fluorination borate and add into above-mentioned cushioning liquid, stir
To being completely dissolved；Point sample is carried out to bilirubin detection zone by solution is prepared using pipettor or auto sample applicator, sample size is
0.5~1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by UBG detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take 0.35g paraxylene diazonium tetrafluoride boron and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using pipettor or auto sample applicator carries out point sample to UBG detection zone, and sample size is 0.5~
1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by nitrite detection block
0.08g arsanilic acids are weighed, 0.09gN- (naphthalidine)-ethylenediamine, 0.01g sodium metaperiodates are added to 10ml
Ultra-pure water in, be stirred well to and be completely dissolved；Solution will be prepared using pipettor or auto sample applicator to detect nitrite
Region carries out point sample, and sample size is 0.5~1 μ l, 30~40 DEG C of drying after the completion of point sample；
It is prepared by white blood cell detection block
0.25g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L sodium hydrate regulator solutions PH to 9.0, takes 0.04g pyrrole esters, 0.02g weights
Nitrogen salt, 0.075g imidazoles is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；To be matched somebody with somebody using pipettor or auto sample applicator
Solution processed carries out point sample to white blood cell detection region, and sample size is 0.5~1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by ascorbic acid detection block
0.08g2 is weighed, 6- dichloropheno-lindophenols add into the ultra-pure water of 10ml, are stirred well to and are completely dissolved；Use shifting
Liquid device or auto sample applicator will prepare solution Ascorbic Acid detection zone and carry out point sample, and sample size is 0.5~1 μ l, and point sample is complete
Into latter 40~60 DEG C drying；
It is prepared by occult blood detection block
0.17g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 6.5, takes 0.012g
Urea peroxide, 0.08g o-tolidines, 0.05g 2- acetic acid amino-thiazols, 2.6g Potassiumiodates are added into above-mentioned cushioning liquid,
Stirring is to being completely dissolved；Point sample, sample size are carried out to occult blood detection zone by solution is prepared using pipettor or auto sample applicator
It is 0.5~1 μ l, 30~45 DEG C of drying after the completion of point sample；
It is prepared by uric acid detection block
0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g
4-AA, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned
In cushioning liquid, stirring is to being completely dissolved；Uric acid detection zone is carried out by solution is prepared using pipettor or auto sample applicator
Point sample, sample size is 0.5~1 μ l, 25~35 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 12, take 0.05g potassium chloride, 0.02g polyvinylpyrrolidones, 0.001g is orange
G, 0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Use pipettor or automatic
Point sample instrument will prepare solution and point sample carried out to creatinine detection zone, and sample size is 0.5~1 μ l, 40~60 DEG C of bakings after the completion of point sample
It is dry；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs, 0.1ml Triton x- to 9.5
100,0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；To be matched somebody with somebody using pipettor or auto sample applicator
Solution processed carries out point sample to creatinine detection zone, and sample size is 0.5~1 μ l, 60~80 DEG C of drying after the completion of point sample；
3. the test paper that will be prepared is fixed on bottom with three substrates in hole.
The beneficial effects of the invention are as follows：The invention provides a kind of urinalysis test paper that can simultaneously detect multiple projects
And preparation method, each detection block by hydrophobic barrier be spaced, urine is dropped to by each detection block by open-work, make urine with
Reagent in each detection block is reacted simultaneously, and multiple test modules are integrated on same miniature test paper, not using only urine
Liquid measure is few, and whole detection process can be completed in moment, without the careful dropwise addition urine successively as routine Urine test paper bar,
Testing cost can be greatly reduced while improving detection efficiency.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the structural representation of bottom of the invention；
In figure：1- imbibitions area；2- detection blocks；3- spacing blocks；4- substrates；5- baffle plates；6- sample holes；
It is described in detail referring to the drawings below with reference to embodiments of the invention.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and examples：
Embodiment 1
As shown in Figure 1, 2, a kind of urinalysis test paper, including test paper and substrate 4, the test paper include three rectangular bars
The imbibition area 1 of shape and four rectangular strip detection zones, four imbibition areas 1 and three detection intervals are every row
Row, each detection zone is made up of four detection blocks 2 and five spacing blocks 3, five spacing blocks 3 and four detection blocks 2
It is spaced,
The imbibition area 1 is made up of polyester fiber film, and the spacing block 3 is made up of photoresist,
The substrate 4 is padded on the test paper lower section, and the center of the substrate 4 is provided with three penetrating sample holes 6, described in three
Sample holes 6 are corresponded respectively at the different imbibition areas 1,
The edge of the substrate 4 is provided with baffle plate 5 upward, and the test paper is fixed on the substrate 4 by the baffle plate 5
On.
A kind of preparation method of urinalysis test paper, its step is as follows：
1. absorbent material is cut to size 10mm × 7mm as test paper base material, is dripped using curable polymer and is dipped into examination
On paper base material and it is allowed to polymerizing curable and is formed act as the spacing block 3 of hydrophobic barrier, test paper base material is separated into three rows, four are often arranged
Individual detection block 2, makes the surrounding of each detection block 2 be surrounded by hydrophobic barrier；
2. chemical reagent dropwise addition and treatment are carried out in 12 detection blocks 2 respectively, this 12 detection blocks 2 is made respectively
It is standby into the item detection block for being used to detect disparity items；
Described item detection block is glucose detection block, acid-base value detection block, protein detection block, microalbumin inspection
Survey block, proportion detection block, ketoboidies detection block, white blood cell detection block, ascorbic acid detection block, occult blood detection block, uric acid detection block,
Creatinine detection block, calcium detection block, the preparation method of projects detection block are specially：
It is prepared by glucose detection block
Weigh 0.19g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena adjusts solution PH to 6.0, takes 0.025g tetramethyl benzidines, and 920u glucose oxidases, 19u peroxidase is added
To in above-mentioned cushioning liquid, stirring is to being completely dissolved；Point sample is carried out to glucose detection region by solution is prepared using pipettor,
Sample size is 0.5 μ l, 25 DEG C of drying after the completion of point sample；
It is prepared by acid-base value detection block
1.2g methyl reds are weighed, 0.8g bromothymol blues, 0.8ml ethanol is added into the ultra-pure water of 10ml, fully stirred
Mix to being completely dissolved；To prepare solution using pipettor carries out point sample to acid-base value detection zone, and sample size is 0.5 μ l, point sample
After the completion of 50 DEG C drying；
It is prepared by protein detection block
Weigh 0.095g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L
NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.8ml ethanol, 0.025g polyvinyl alcohol, 0.033g tetrabromo phenol to 2.0
Indigo plant is added in above-mentioned cushioning liquid, and stirring is to being completely dissolved；Protein detection region is entered by solution is prepared using pipettor
Row point sample, sample size is 0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by microalbumin detection block
Weigh 1.9g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and 1mol/L hydrochloric acid conditioning solutions PH to 2.0, take 0.01g sulfonephthalein dyestuffs and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using pipettor carries out point sample to microalbumin detection zone, and sample size is 0.5 μ l, and point sample is complete
Into latter 50 DEG C drying；
It is prepared by proportion detection block
The metaphosphoric acids of 0.012g tri- are weighed, 0.018g bromothymol blues and 0.05g tetramethylammonium hydroxides add surpassing to 10ml
In pure water, it is stirred well to and is completely dissolved, will prepare solution contrast re-detection region using pipettor carries out point sample, and sample size is
0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by ketoboidies detection block
0.5g NaOH is weighed, 0.125g sodium nitroprussides are added into the ultra-pure water of 10ml, are stirred well to
It is completely dissolved, will prepare solution using pipettor carries out point sample to ketoboidies detection zone, and sample size is 0.5 μ l, after the completion of point sample
35 DEG C of drying；
It is prepared by white blood cell detection block
0.25g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L sodium hydrate regulator solutions PH to 9.0, takes 0.04g pyrrole esters, 0.02g weights
Nitrogen salt, 0.075g imidazoles is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；It will be thin solution dialogue will to be prepared using pipettor
Born of the same parents' detection zone carries out point sample, and sample size is 0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by ascorbic acid detection block
0.08g2 is weighed, 6- dichloropheno-lindophenols add into the ultra-pure water of 10ml, are stirred well to and are completely dissolved；Use shifting
Liquid device will prepare solution Ascorbic Acid detection zone and carry out point sample, and sample size is 0.5 μ l, 40 DEG C of drying after the completion of point sample；
It is prepared by occult blood detection block
0.17g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 6.5, takes 0.012g
Urea peroxide, 0.08g o-tolidines, 0.05g 2- acetic acid amino-thiazols, 2.6g Potassiumiodates are added into above-mentioned cushioning liquid,
Stirring is to being completely dissolved；To prepare solution using pipettor carries out point sample to occult blood detection zone, and sample size is 0.5 μ l, point sample
After the completion of 30 DEG C drying；
It is prepared by uric acid detection block
0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g
4-AA, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned
In cushioning liquid, stirring is to being completely dissolved；Point sample, sample are carried out to uric acid detection zone by solution is prepared using auto sample applicator
It is 1 μ l to measure, 35 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 12, take 0.05g potassium chloride, 0.02g polyvinylpyrrolidones, 0.001g is orange
G, 0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Will using auto sample applicator
Preparing solution carries out point sample to creatinine detection zone, and sample size is 1 μ l, 60 DEG C of drying after the completion of point sample；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs, 0.1ml Triton x- to 9.5
100,0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution pair will be prepared using auto sample applicator
Creatinine detection zone carries out point sample, and sample size is 1 μ l, 80 DEG C of drying after the completion of point sample；
3. the test paper that will be prepared is fixed on bottom with three substrates 4 of sample holes 6.
Embodiment 2
A kind of urinalysis test paper, including test paper and substrate 4, the test paper include three imbibition areas of rectangular strip
1 and four rectangular strip detection zones, four imbibition areas 1 are spaced with three detection zones, each detection zone
It is made up of five detection blocks 2 and six spacing blocks 3, five spacing blocks 3 and six detection blocks 2 are spaced,
The imbibition area 1 is made up of glass fibre membrane, and the spacing block 3 is made up of curable polymer,
The substrate 4 is padded on the test paper lower section, and the center of the substrate 4 is provided with three penetrating sample holes 6, described in three
Sample holes 6 are corresponded respectively at the different imbibition areas 1,
The edge of the substrate 4 is provided with baffle plate 5 upward, and the test paper is fixed on the substrate 4 by the baffle plate 5
On.
A kind of preparation method of urinalysis test paper, its step is as follows：
1. glass fibre membrane is cut to size 10mm × 7mm as test paper base material, is dripped using curable polymer and is dipped into
On test paper base material and it is allowed to polymerizing curable and is formed act as the spacing block 3 of hydrophobic barrier, test paper base material is separated into three rows, is often arranged
The detection block 2 of five, makes the surrounding of each detection block 2 be surrounded by hydrophobic barrier；
2. chemical reagent dropwise addition and treatment are carried out in 15 detection blocks 2 respectively, this 15 detection blocks 2 is made respectively
It is standby into the item detection block for being used to detect disparity items；
Described item detection block is glucose detection block, acid-base value detection block, protein detection block, microalbumin inspection
Survey block, proportion detection block, ketoboidies detection block, bilirubin detection block, UBG detection block, nitrite detection block, leucocyte inspection
Block, ascorbic acid detection block, occult blood detection block, uric acid detection block, creatinine detection block, calcium detection block are surveyed, projects detection block
Preparation method is specially：
It is prepared by glucose detection block
Weigh 0.19g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena adjusts solution PH to 6.0, takes 0.025g tetramethyl benzidines, and 920u glucose oxidases, 19u peroxidase is added
To in above-mentioned cushioning liquid, stirring is to being completely dissolved；Point sample is carried out to glucose detection region by solution is prepared using pipettor,
Sample size is 0.5 μ l, 25 DEG C of drying after the completion of point sample；
It is prepared by acid-base value detection block
1.2g methyl reds are weighed, 0.8g bromothymol blues, 0.8ml ethanol is added into the ultra-pure water of 10ml, fully stirred
Mix to being completely dissolved；To prepare solution using pipettor carries out point sample to acid-base value detection zone, and sample size is 0.5 μ l, point sample
After the completion of 50 DEG C drying；
It is prepared by protein detection block
Weigh 0.095g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L
NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.8ml ethanol, 0.025g polyvinyl alcohol, 0.033g tetrabromo phenol to 2.0
Indigo plant is added in above-mentioned cushioning liquid, and stirring is to being completely dissolved；Protein detection region is entered by solution is prepared using pipettor
Row point sample, sample size is 0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by microalbumin detection block
Weigh 1.9g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and 1mol/L hydrochloric acid conditioning solutions PH to 2.0, take 0.01g sulfonephthalein dyestuffs and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using pipettor carries out point sample to microalbumin detection zone, and sample size is 0.5 μ l, and point sample is complete
Into latter 50 DEG C drying；
It is prepared by proportion detection block
The metaphosphoric acids of 0.012g tri- are weighed, 0.018g bromothymol blues and 0.05g tetramethylammonium hydroxides add surpassing to 10ml
In pure water, it is stirred well to and is completely dissolved, will prepare solution contrast re-detection region using pipettor carries out point sample, and sample size is
0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by ketoboidies detection block
0.5g NaOH is weighed, 0.125g sodium nitroprussides are added into the ultra-pure water of 10ml, are stirred well to
It is completely dissolved, will prepare solution using pipettor carries out point sample to ketoboidies detection zone, and sample size is 0.5 μ l, after the completion of point sample
35 DEG C of drying；
It is prepared by bilirubin detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take the dichloro diazols of 0.35g 2,6 fluorination borate and add into above-mentioned cushioning liquid, stir
To being completely dissolved；To prepare solution using pipettor carries out point sample to bilirubin detection zone, and sample size is 0.5 μ l, and point sample is complete
Into latter 50 DEG C drying；
It is prepared by UBG detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take 0.35g paraxylene diazonium tetrafluoride boron and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using pipettor carries out point sample to UBG detection zone, and sample size is 0.5 μ l, after the completion of point sample
50 DEG C of drying；
It is prepared by nitrite detection block
0.08g arsanilic acids are weighed, 0.09gN- (naphthalidine)-ethylenediamine, 0.01g sodium metaperiodates are added to 10ml
Ultra-pure water in, be stirred well to and be completely dissolved；Point sample is carried out to nitrite detection zone by solution is prepared using pipettor,
Sample size is 0.5 μ l, 30 DEG C of drying after the completion of point sample；
It is prepared by white blood cell detection block
0.25g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L sodium hydrate regulator solutions PH to 9.0, takes 0.04g pyrrole esters, 0.02g weights
Nitrogen salt, 0.075g imidazoles is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；It will be thin solution dialogue will to be prepared using pipettor
Born of the same parents' detection zone carries out point sample, and sample size is 0.5 μ l, 50 DEG C of drying after the completion of point sample；
It is prepared by ascorbic acid detection block
0.08g2 is weighed, 6- dichloropheno-lindophenols add into the ultra-pure water of 10ml, are stirred well to and are completely dissolved；Use shifting
Liquid device will prepare solution Ascorbic Acid detection zone and carry out point sample, and sample size is 0.5 μ l, 40 DEG C of drying after the completion of point sample；
It is prepared by occult blood detection block
0.17g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 6.5, takes 0.012g
Urea peroxide, 0.08g o-tolidines, 0.05g 2- acetic acid amino-thiazols, 2.6g Potassiumiodates are added into above-mentioned cushioning liquid,
Stirring is to being completely dissolved；To prepare solution using pipettor carries out point sample to occult blood detection zone, and sample size is 0.5 μ l, point sample
After the completion of 30 DEG C drying；
It is prepared by uric acid detection block
0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g
4-AA, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned
In cushioning liquid, stirring is to being completely dissolved；Uric acid detection zone is carried out by solution is prepared using pipettor or auto sample applicator
Point sample, sample size is 0.5 μ l, 25 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 12, take 0.05g potassium chloride, 0.02g polyvinylpyrrolidones, 0.001g is orange
G, 0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；To be prepared using pipettor
Solution carries out point sample to creatinine detection zone, and sample size is 0.5 μ l, 40 DEG C of drying after the completion of point sample；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs, 0.1ml Triton x- to 9.5
100,0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution will be prepared to creatinine using pipettor
Detection zone carries out point sample, and sample size is 0.5 μ l, 60 DEG C of drying after the completion of point sample；
3. the test paper that will be prepared is fixed on bottom with three substrates 4 of sample holes 6；
Embodiment 3
As shown in Figure 1, 2, a kind of urinalysis test paper, including test paper and substrate 4, the test paper include three rectangular bars
The imbibition area 1 of shape and four rectangular strip detection zones, four imbibition areas 1 and three detection intervals are every row
Row, each detection zone is made up of five detection blocks 2 and six spacing blocks 3, five spacing blocks 3 and six detection blocks 2
It is spaced,
The imbibition area 1 is made up of glass fibre membrane, and the spacing block 3 is made up of curable polymer,
The substrate 4 is padded on the test paper lower section, and the center of the substrate 4 is provided with three penetrating sample holes 6, described in three
Sample holes 6 are corresponded respectively at the different imbibition areas 1,
The edge of the substrate 4 is provided with baffle plate 5 upward, and the test paper is fixed on the substrate 4 by the baffle plate 5
On.
A kind of preparation method of urinalysis test paper, its step is as follows：
1. absorbent material is cut to size 10mm × 7mm as test paper base material, is dripped using hydrophobic material and is dipped into test paper base
On material and it is allowed to polymerizing curable and is formed act as the spacing block 3 of hydrophobic barrier, test paper base material is separated into three rows, four are often arranged
Detection block 2, makes the surrounding of each detection block 2 be surrounded by hydrophobic barrier；
2. chemical reagent dropwise addition and treatment are carried out in 15 detection blocks 2 respectively, this 15 detection blocks 2 is made respectively
It is standby into the item detection block for being used to detect disparity items；
Described item detection block is glucose detection block, acid-base value detection block, protein detection block, microalbumin inspection
Survey block, proportion detection block, ketoboidies detection block, bilirubin detection block, UBG detection block, nitrite detection block, leucocyte inspection
Block, ascorbic acid detection block, occult blood detection block, uric acid detection block, creatinine detection block, calcium detection block are surveyed, projects detection block
Preparation method is specially：
It is prepared by glucose detection block
Weigh 0.19g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena adjusts solution PH to 6.0, takes 0.025g tetramethyl benzidines, and 920u glucose oxidases, 19u peroxidase is added
To in above-mentioned cushioning liquid, stirring is to being completely dissolved；Glucose detection region is carried out by solution is prepared using auto sample applicator
Point sample, sample size is 1 μ l, 35 DEG C of drying after the completion of point sample；
It is prepared by acid-base value detection block
1.2g methyl reds are weighed, 0.8g bromothymol blues, 0.8ml ethanol is added into the ultra-pure water of 10ml, fully stirred
Mix to being completely dissolved；To prepare solution using auto sample applicator carries out point sample to acid-base value detection zone, and sample size is 1 μ l, point
80 DEG C of drying after the completion of sample；
It is prepared by protein detection block
Weigh 0.095g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L
NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.8ml ethanol, 0.025g polyvinyl alcohol, 0.033g tetrabromo phenol to 3.0
Indigo plant is added in above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution will be prepared to protein detection area using auto sample applicator
Domain carries out point sample, and sample size is 1 μ l, 80 DEG C of drying after the completion of point sample；
It is prepared by microalbumin detection block
Weigh 1.9g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and 1mol/L hydrochloric acid conditioning solutions PH to 3.0, take 0.01g sulfonephthalein dyestuffs and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using auto sample applicator carries out point sample to microalbumin detection zone, and sample size is 1 μ l, point sample
After the completion of 70 DEG C drying；
It is prepared by proportion detection block
The metaphosphoric acids of 0.012g tri- are weighed, 0.018g bromothymol blues and 0.05g tetramethylammonium hydroxides add surpassing to 10ml
In pure water, it is stirred well to and is completely dissolved, will prepare solution contrast re-detection region using auto sample applicator carries out point sample, sample
It is 1 μ l to measure, 80 DEG C of drying after the completion of point sample；
It is prepared by ketoboidies detection block
0.5g NaOH is weighed, 0.125g sodium nitroprussides are added into the ultra-pure water of 10ml, are stirred well to
It is completely dissolved, will prepare solution using auto sample applicator carries out point sample to ketoboidies detection zone, and sample size is 1 μ l, and point sample is completed
50 DEG C of drying afterwards；
It is prepared by bilirubin detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take 0.35g 2,6- dichloros diazol fluorination borate is added into above-mentioned cushioning liquid, stirred
To being completely dissolved；To prepare solution using auto sample applicator carries out point sample to bilirubin detection zone, and sample size is 1 μ l, point sample
After the completion of 70 DEG C drying；
It is prepared by UBG detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L hydrogen-oxygens
Change sodium and adjust solution PH to 5.0, take 0.35g paraxylene diazonium tetrafluoride boron and add into above-mentioned cushioning liquid, stirring is to complete
CL；To prepare solution using auto sample applicator carries out point sample to UBG detection zone, and sample size is 1 μ l, and point sample is completed
70 DEG C of drying afterwards；
It is prepared by nitrite detection block
0.08g arsanilic acids are weighed, 0.09gN- (naphthalidine)-ethylenediamine, 0.01g sodium metaperiodates are added to 10ml
Ultra-pure water in, be stirred well to and be completely dissolved；Nitrite detection zone is carried out by solution is prepared using auto sample applicator
Point sample, sample size is 1 μ l, 40 DEG C of drying after the completion of point sample；
It is prepared by white blood cell detection block
0.25g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L sodium hydrate regulator solutions PH to 9.0, takes 0.04g pyrrole esters, 0.02g weights
Nitrogen salt, 0.075g imidazoles is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution pair will be prepared using auto sample applicator
White blood cell detection region carries out point sample, and sample size is 1 μ l, 70 DEG C of drying after the completion of point sample；
It is prepared by ascorbic acid detection block
0.08g2 is weighed, 6- dichloropheno-lindophenols add into the ultra-pure water of 10ml, are stirred well to and are completely dissolved；Using certainly
Dynamic point sample instrument will prepare solution Ascorbic Acid detection zone and carry out point sample, and sample size is 1 μ l, 60 DEG C of drying after the completion of point sample；
It is prepared by occult blood detection block
0.17g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 6.5, takes 0.012g
Urea peroxide, 0.08g o-tolidines, 0.05g 2- acetic acid amino-thiazols, 2.6g Potassiumiodates are added into above-mentioned cushioning liquid,
Stirring is to being completely dissolved；To prepare solution using auto sample applicator carries out point sample to occult blood detection zone, and sample size is 1 μ l, point
45 DEG C of drying after the completion of sample；
It is prepared by uric acid detection block
0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g
4-AA, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned
In cushioning liquid, stirring is to being completely dissolved；Point sample, sample are carried out to uric acid detection zone by solution is prepared using auto sample applicator
It is 1 μ l to measure, 35 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 12, take 0.05g potassium chloride, 0.02g polyvinylpyrrolidones, 0.001g is orange
G, 0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Will using auto sample applicator
Preparing solution carries out point sample to creatinine detection zone, and sample size is 1 μ l, 60 DEG C of drying after the completion of point sample；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs, 0.1ml Triton x- to 9.5
100,0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution pair will be prepared using auto sample applicator
Creatinine detection zone carries out point sample, and sample size is 1 μ l, 80 DEG C of drying after the completion of point sample；
It is prepared by uric acid detection block
0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride is weighed to add into the ultra-pure water of 10ml,
It is stirred well to and is completely dissolved, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g
4-AA, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned
In cushioning liquid, stirring is to being completely dissolved；Point sample, sample are carried out to uric acid detection zone by solution is prepared using auto sample applicator
It is 1 μ l to measure, 35 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 12, take 0.05g potassium chloride, 0.02g polyvinylpyrrolidones, 0.001g is orange
G, 0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Will using auto sample applicator
Preparing solution carries out point sample to creatinine detection zone, and sample size is 1 μ l, 60 DEG C of drying after the completion of point sample；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen
Sodium oxide molybdena and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs, 0.1ml Triton x- to 9.5
100,0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution pair will be prepared using auto sample applicator
Creatinine detection zone carries out point sample, and sample size is 1 μ l, 80 DEG C of drying after the completion of point sample；
3. the test paper that will be prepared is fixed on bottom with three substrates 4 of sample holes 6.
When the present invention is used, urine to be measured is added in the sample holes 6 at the back of substrate 4, by the capillarity in imbibition area 1
Sample is distributed to whole test paper, testing liquid is immersed in each test block 2, by chemistry in urine to be measured and test block 2
The reaction of reagent, makes the surface of test block 2 show different pattern and colors.
The present invention is exemplarily described above in conjunction with accompanying drawing, it is clear that the present invention is implemented and do not receive aforesaid way
Limitation, as long as employing method of the present invention design and the various improvement that carry out of technical scheme, or not improved direct application
In other occasions, within protection scope of the present invention.
Claims (5)
1. a kind of urinalysis test paper, it is characterised in that including test paper and substrate (4), the test paper includes multiple rectangular bars
The imbibition area (1) of shape and detection zone, multiple imbibition areas (1) are spaced with multiple detection zones, each detection zone
It is made up of multiple detection blocks (2) and multiple spacing blocks (3), multiple spacing blocks (3) and multiple detection blocks (2) interval are arranged
Row,
The imbibition area (1) is made up of absorbent material, and the spacing block (3) is made up of hydrophobic material,
The substrate (4) is padded on the test paper lower section, and substrate (4) center is provided with penetrating sample holes (6).
2. a kind of urinalysis test paper according to claim 1, it is characterised in that the sample holes (6) have three, three
The sample holes (6) correspond respectively to different imbibition area (1) places.
3. a kind of urinalysis test paper according to claim 1, it is characterised in that substrate (4) the edge court
Baffle plate (5) is provided with, the test paper is fixed on the substrate (4) by the baffle plate (5).
4. a kind of urinalysis test paper according to claim 1, it is characterised in that prepare the water suction of the imbibition area (1)
Material is the one kind in blotting paper, glass fibre membrane and polyester fiber film；The hydrophobic material of the spacing block (3) is prepared for that can consolidate
One kind in fluidized polymer and photoresist.
5. a kind of preparation method of urinalysis test paper as claimed in claim 1, it is characterised in that its step is as follows：
1. absorbent material is cut to size 10mm × 7mm as test paper base material, is dripped using hydrophobic material and is dipped on test paper base material
And the spacing block (3) that polymerizing curable formation act as hydrophobic barrier is allowed to, and test paper base material is separated into multiple detection blocks (2), make
The surrounding of each detection block (2) is surrounded by hydrophobic barrier；
2. chemical reagent dropwise addition and treatment are carried out in multiple detection blocks (2) respectively, multiple detection blocks (2) is prepared into use respectively
To detect the item detection block of disparity items；
Described item detection block is selected from glucose detection block, acid-base value detection block, protein detection block, microalbumin detection
Block, proportion detection block, ketoboidies detection block, bilirubin detection block, UBG detection block, nitrite detection block, white blood cell detection
Block, ascorbic acid detection block, occult blood detection block, uric acid detection block, creatinine detection block, calcium detection block, the system of projects detection block
Preparation Method is specially：
It is prepared by glucose detection block
Weigh 0.19g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydroxides
Sodium adjusts solution PH to 6.0, takes 0.025g tetramethyl benzidines, and 920u glucose oxidases, 19u peroxidase adds supreme
In stating cushioning liquid, stirring is to being completely dissolved；Solution will be prepared to glucose detection region using pipettor or auto sample applicator
Point sample is carried out, sample size is 0.5~1 μ l, 25~35 DEG C of drying after the completion of point sample；
It is prepared by acid-base value detection block
1.2g methyl reds are weighed, 0.8g bromothymol blues, 0.8ml ethanol is added into the ultra-pure water of 10ml, is stirred well to
It is completely dissolved；Point sample is carried out to acid-base value detection zone by solution is prepared using pipettor or auto sample applicator, sample size is 0.5
~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by protein detection block
Weigh 0.095g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydrogen-oxygens
Change sodium and 0.1mol/L hydrochloric acid conditioning solutions PH to 2.0~3.0, take 0.8ml ethanol, 0.025g polyvinyl alcohol, 0.033g tetrabromos
Phenol is blue to be added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Solution will be prepared to egg using pipettor or auto sample applicator
White matter detection zone carries out point sample, and sample size is 0.5~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by microalbumin detection block
Weigh 1.9g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L NaOH
With 1mol/L hydrochloric acid conditioning solutions PH to 2.0~3.0, take 0.01g sulfonephthalein dyestuffs and add into above-mentioned cushioning liquid, stirring is to complete
CL；Point sample is carried out to microalbumin detection zone by solution is prepared using pipettor or auto sample applicator, sample size is
0.5~1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by proportion detection block
The metaphosphoric acids of 0.012g tri- are weighed, 0.018g bromothymol blues and 0.05g tetramethylammonium hydroxides are added to the ultra-pure water of 10ml
In, it is stirred well to and is completely dissolved, will prepare solution contrast re-detection region using pipettor or auto sample applicator carries out point sample,
Sample size is 0.5~1 μ l, 50~80 DEG C of drying after the completion of point sample；
It is prepared by ketoboidies detection block
0.5g NaOH is weighed, 0.125g sodium nitroprussides are added into the ultra-pure water of 10ml, be stirred well to completely
Dissolving, will prepare solution using pipettor or auto sample applicator carries out point sample to ketoboidies detection zone, and sample size is 0.5~1 μ l,
35~50 DEG C of drying after the completion of point sample；
It is prepared by bilirubin detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L NaOH
Regulation solution PH takes the dichloro diazols of 0.35g 2,6 fluorination borate and adds into above-mentioned cushioning liquid to 5.0, and stirring is to complete
CL；To prepare solution using pipettor or auto sample applicator carries out point sample to bilirubin detection zone, and sample size is 0.5~
1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by UBG detection block
Weigh 0.95g citric acids to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 1mol/L NaOH
Regulation solution PH to 5.0, take 0.35g paraxylene diazonium tetrafluoride boron and add into above-mentioned cushioning liquid, stirring to completely it is molten
Solution；To prepare solution using pipettor or auto sample applicator carries out point sample to UBG detection zone, and sample size is 0.5~1 μ l,
50~70 DEG C of drying after the completion of point sample；
It is prepared by nitrite detection block
0.08g arsanilic acids, 0.09gN- (naphthalidine)-ethylenediamine are weighed, 0.01g sodium metaperiodates add surpassing to 10ml
In pure water, it is stirred well to and is completely dissolved；Solution will be prepared to nitrite detection zone using pipettor or auto sample applicator
Point sample is carried out, sample size is 0.5~1 μ l, 30~40 DEG C of drying after the completion of point sample；
It is prepared by white blood cell detection block
Weigh 0.25g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride to add into the ultra-pure water of 10ml, fully
Stirring, using 0.1mol/L sodium hydrate regulator solutions PH to 9.0, takes 0.04g pyrrole esters to being completely dissolved, 0.02g diazols,
0.075g imidazoles is added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；To prepare molten using pipettor or auto sample applicator
Liquid carries out point sample to white blood cell detection region, and sample size is 0.5~1 μ l, 50~70 DEG C of drying after the completion of point sample；
It is prepared by ascorbic acid detection block
0.08g2 is weighed, 6- dichloropheno-lindophenols add into the ultra-pure water of 10ml, are stirred well to and are completely dissolved；Use pipettor
Or auto sample applicator will prepare solution Ascorbic Acid detection zone and carry out point sample, sample size is 0.5~1ul, after the completion of point sample
40~60 DEG C of drying；
It is prepared by occult blood detection block
Weigh 0.17g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride to add into the ultra-pure water of 10ml, fully
Stirring, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 6.5, takes 0.012g peroxides to being completely dissolved
Change urea, 0.08g o-tolidines, 0.05g 2- acetic acid amino-thiazols, 2.6g Potassiumiodates are added into above-mentioned cushioning liquid, stir
To being completely dissolved；Point sample is carried out to occult blood detection zone by solution is prepared using pipettor or auto sample applicator, sample size is 0.5
~1 μ l, 30~45 DEG C of drying after the completion of point sample；
It is prepared by uric acid detection block
Weigh 0.20g disodium hydrogen phosphates, 0.03g sodium dihydrogen phosphates, 0.09g sodium chloride to add into the ultra-pure water of 10ml, fully
Stirring, using 0.1mol/L NaOH and 0.1mol/L hydrochloric acid conditioning solutions PH to 7.2, takes 0.06g 4- ammonia to being completely dissolved
Base antipyrine, 0.04g 2- hydroxyl -3,5- dichloro benzosulfonic acids, 15u uricases, 25u peroxidase is added to above-mentioned buffering
In solution, stirring is to being completely dissolved；Point sample is carried out to uric acid detection zone by solution is prepared using pipettor or auto sample applicator,
Sample size is 0.5~1 μ l, 25~35 DEG C of drying after the completion of point sample；
It is prepared by creatinine detection block
Weigh 0.12g boric acid to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L NaOH
With 0.1mol/L hydrochloric acid conditioning solutions PH to 12,0.05g potassium chloride is taken, 0.02g polyvinylpyrrolidones, 0.001g orange Gs,
0.005g 3.5- dinitrobenzoic acids are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；Using pipettor or from moving point
Sample instrument will prepare solution and point sample carried out to creatinine detection zone, and sample size is 0.5~1 μ l, 40~60 DEG C of drying after the completion of point sample；
It is prepared by calcium detection block
Weigh 0.19g lemon yellows to add into the ultra-pure water of 10ml, be stirred well to and be completely dissolved, use 0.1mol/L hydroxides
Sodium and 0.1mol/L hydrochloric acid conditioning solutions PH take 0.035g OCPCs to 9.5,0.1ml Triton x-100,
0.035g EGTA are added into above-mentioned cushioning liquid, and stirring is to being completely dissolved；To prepare molten using pipettor or auto sample applicator
Liquid carries out point sample to creatinine detection zone, and sample size is 0.5~1 μ l, 60~80 DEG C of drying after the completion of point sample；
3. the test paper that will be prepared is fixed on bottom with three substrates in hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611252827.7A CN106770251B (en) | 2016-12-30 | 2016-12-30 | Urine analysis test paper and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611252827.7A CN106770251B (en) | 2016-12-30 | 2016-12-30 | Urine analysis test paper and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106770251A true CN106770251A (en) | 2017-05-31 |
| CN106770251B CN106770251B (en) | 2023-08-18 |
Family
ID=58928544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611252827.7A Active CN106770251B (en) | 2016-12-30 | 2016-12-30 | Urine analysis test paper and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106770251B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187946A (en) * | 2018-10-22 | 2019-01-11 | 湖南达道生物工程有限公司 | A kind of foldable colloidal gold/immunofluorescence test device and test method |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| CN110514820A (en) * | 2019-08-27 | 2019-11-29 | 黄连生 | A kind of portable urine test paper reducing interference |
| CN110521613A (en) * | 2019-09-29 | 2019-12-03 | 山东猫寵宠物有限责任公司 | Cat litter and preparation method thereof |
| CN112014389A (en) * | 2020-09-10 | 2020-12-01 | 吉林基蛋生物科技有限公司 | Ascorbic acid interference-based urine occult blood test paper and preparation method thereof |
| CN112285367A (en) * | 2020-12-24 | 2021-01-29 | 中生北控生物科技股份有限公司 | Kit for detecting calcium ions and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1319762A (en) * | 2000-01-21 | 2001-10-31 | 和光纯药工业株式会社 | Method for making multi-item tester and the tester |
| CN202614759U (en) * | 2012-05-08 | 2012-12-19 | 艾康生物技术(杭州)有限公司 | Detection device (III) |
| CN103808714A (en) * | 2012-11-05 | 2014-05-21 | 郑兆珉 | Biomedical detection device |
| CN205388575U (en) * | 2016-03-02 | 2016-07-20 | 长沙展讯信息科技有限公司 | Test paper detects card |
| CN206479459U (en) * | 2016-12-30 | 2017-09-08 | 天津果实科技有限公司 | A kind of urinalysis test paper |
-
2016
- 2016-12-30 CN CN201611252827.7A patent/CN106770251B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1319762A (en) * | 2000-01-21 | 2001-10-31 | 和光纯药工业株式会社 | Method for making multi-item tester and the tester |
| CN202614759U (en) * | 2012-05-08 | 2012-12-19 | 艾康生物技术(杭州)有限公司 | Detection device (III) |
| CN103808714A (en) * | 2012-11-05 | 2014-05-21 | 郑兆珉 | Biomedical detection device |
| CN205388575U (en) * | 2016-03-02 | 2016-07-20 | 长沙展讯信息科技有限公司 | Test paper detects card |
| CN206479459U (en) * | 2016-12-30 | 2017-09-08 | 天津果实科技有限公司 | A kind of urinalysis test paper |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187946A (en) * | 2018-10-22 | 2019-01-11 | 湖南达道生物工程有限公司 | A kind of foldable colloidal gold/immunofluorescence test device and test method |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| CN110514820A (en) * | 2019-08-27 | 2019-11-29 | 黄连生 | A kind of portable urine test paper reducing interference |
| CN110521613A (en) * | 2019-09-29 | 2019-12-03 | 山东猫寵宠物有限责任公司 | Cat litter and preparation method thereof |
| CN110521613B (en) * | 2019-09-29 | 2021-09-10 | 山东猫寵宠物有限责任公司 | Cat litter |
| CN112014389A (en) * | 2020-09-10 | 2020-12-01 | 吉林基蛋生物科技有限公司 | Ascorbic acid interference-based urine occult blood test paper and preparation method thereof |
| CN112285367A (en) * | 2020-12-24 | 2021-01-29 | 中生北控生物科技股份有限公司 | Kit for detecting calcium ions and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106770251B (en) | 2023-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106770251A (en) | A kind of urinalysis test paper and preparation method thereof | |
| AU2004200506B2 (en) | Method for Reducing Effect of Hematocrit on Measurement of an Analyte in Whole Blood, and Test Kit and Test Article Useful in the Method | |
| CN108745428B (en) | Multi-channel three-dimensional microfluidic paper chip and preparation method thereof | |
| US6511814B1 (en) | Method and device for detecting analytes in fluids | |
| CN101128599A (en) | Diffusion and enzyme layer for an enzyme-based sensor application | |
| CN105021596A (en) | Concentration gradient based dry chemical test strip with multiple layers of films | |
| US9670524B2 (en) | Test elements for determining an analyte concentration that include correction information for at least one interfering variable | |
| CN101865853B (en) | Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof | |
| WO2009152373A1 (en) | Hybrid strip | |
| US20160245811A1 (en) | Systems and methods for monitoring biological fluids | |
| GB2528930A (en) | Water sample analysis kit | |
| CN110777191A (en) | Dry tablet reagent for quantitative detection of uric acid | |
| JP2005253412A (en) | Microwell array chip, method for producing the same and method for assaying specimen | |
| CN103217521B (en) | The manufacture method of dry reagent, dry reagent and use its analyzer | |
| US20150147769A1 (en) | Method of testing sample and microfluidic device | |
| CN102419366A (en) | Dry chemical quantitative test strip with interference removal, alanine transaminase or aspartate transaminase quantitative test strip and test method | |
| He et al. | based microfluidic devices based on 3D network polymer hydrogel for the determination of glucose in human whole blood | |
| CN206479459U (en) | A kind of urinalysis test paper | |
| US20050186109A1 (en) | Multilayer analysis element | |
| AU2018200697A1 (en) | Method and device for calibration of biological flux | |
| CN107271500A (en) | A kind of uric acid biochemical analysis dry plate and preparation method thereof and reagent disc | |
| KR101506147B1 (en) | Reagent set, test method of sample, microfluidic device and test apparatus | |
| CN103091264B (en) | Analyzer and analytical approach | |
| JP7025398B2 (en) | Manufacturing method of Uni and No-code test strips | |
| Sieben | Characterization of the permeability of sealing tapes and development of a viscosity measuring technique in shaken reactors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |