CN106770220A - A kind of antibiotics leftover detection kit - Google Patents

A kind of antibiotics leftover detection kit Download PDF

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Publication number
CN106770220A
CN106770220A CN201611020680.9A CN201611020680A CN106770220A CN 106770220 A CN106770220 A CN 106770220A CN 201611020680 A CN201611020680 A CN 201611020680A CN 106770220 A CN106770220 A CN 106770220A
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CN
China
Prior art keywords
detection
paper
culture medium
test paper
antibiotic
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Pending
Application number
CN201611020680.9A
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Chinese (zh)
Inventor
周朱晨
张根义
胡彬
张进
吴念绮
周合
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
100 Olson Jiangsu Food Safety Technology Co Ltd
Original Assignee
100 Olson Jiangsu Food Safety Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 100 Olson Jiangsu Food Safety Technology Co Ltd filed Critical 100 Olson Jiangsu Food Safety Technology Co Ltd
Priority to CN201611020680.9A priority Critical patent/CN106770220A/en
Publication of CN106770220A publication Critical patent/CN106770220A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of antibiotics leftover detection kit, including the detection culture medium control antibiotic standard scraps of paper, detection filter paper, the detection includes antibiotic culture medium dry powder with culture medium, antibiotic culture medium dry powder, detection bacterium bag bacteria suspension containing micrococcus luteus, Bacillus subtillis spore solution, Bacillus cereus gill fungus shape mutation spore solution, the control antibiotic standard scraps of paper are divided into four kinds of Test papers of mycin respectively terramycin Test paper, erythromycin Test paper, penicillin Test paper, kanamycins Test paper, detection filter paper is additionally provided with the kit, the detection filter paper left side is provided with yin and yang attribute detection unit.This kind of antibiotics leftover detection kit can be directed to the Antimicrobial method of the antibiotic formulation rapid screening that this four class is commonly used, and the concentration of detection residual microbial that can be in batches can be detected with various test paper, determine its accuracy.

Description

A kind of antibiotics leftover detection kit
Technical field
The present invention relates to detection kit technical field, specially a kind of antibiotics leftover detection kit.
Background technology
In food security, medicament residue receives much attention.Over nearly 30 years, veterinary drug animal husbandry, culture fishery should With increasingly extensive, its effect in terms of reducing incidence and mortality, growth promotion, improving efficiency of feed utilization and product quality is It is very significant.Beta-lactam, macrolides, aminoglycoside and Tetracyclines are exactly four classes with a long history, important Conventional antimicrobial agents.But, the use of veterinary drug can undoubtedly cause the delay of medicine in animal body to be accumulated, and with residual Mode enters human body and the ecosystem.Harm of the residue of veterinary drug to the mankind and environment is mainly chronic, at a specified future date and cumulative, such as Carcinogenic, development toxicity, in vivo accumulation, immunosupress, sensitization and antibody-resistant bacterium etc..In recent years, with China joined WTO, technology Property trade barrier day aobvious protrude.It is due in breeding process, still suffering from drug abuse or existing using not clear composition medicine etc. As, and careless omission is just easily caused with the pattern that specific aim project carries out detection.At the beginning of 2003, day is originally in China's export roast eel Detection Enrofloxacin, and it is further right by the regulation that " synthetic antibiotic must not be contained in animal derived food " in food hygiene law China's export animal derived food sets threshold.This event undoubtedly detection of veterinary drugs in food work is proposed it is new it is higher will Ask.
Conventional antibiotics leftover detection kit only has a kind of detection method so that testing result is not necessarily accurate, needs To verify again, and conventional antibiotics leftover detection kit intelligence is by dropper instillation reagent drop by drop of trading, Can not once substantial amounts of addition, sometimes seeming fairly time consuming.
The content of the invention
It is an object of the invention to provide a kind of antibiotics leftover detection kit, possesses the work(of available various detection papers Can, solve the problems, such as that a kind of Test paper is inaccurate.
To achieve the above object, the present invention provides following technical scheme:
A kind of antibiotics leftover detection kit, including the detection culture medium control antibiotic standard scraps of paper, detection filter paper Piece, the detection includes antibiotic culture medium dry powder, antibiotic culture medium dry powder with culture medium, and the detection is contained with bacterium bag Micrococcus luteus bacteria suspension, Bacillus subtillis spore solution, Bacillus cereus gill fungus shape mutation spore solution, the control are used The antibiotic standard scraps of paper are divided into four kinds of Test papers of mycin respectively terramycin Test paper, erythromycin Test paper, mould Plain Test paper, kanamycins Test paper, are additionally provided with detection filter paper in the kit, the detection is left with filter paper Side is provided with yin and yang attribute detection unit.
Preferably, liquid droping port is provided with directly over the detection culture medium, liquid droping port top is provided with the liquid cover that inclines, and incline liquid cover It is funnel-form, test paper insertion groove is provided with inside detection culture medium.
Preferably, the antibiotic standard scraps of paper are divided into and are divided into seven concentration cells and are tested.
Preferably, the detection filter paper left side is provided with the fixed bonding die of viscosity.
Compared with prior art, beneficial effects of the present invention are as follows:
The concentration of detection residual microbial that the 1st, can be in batches, can be detected with various test paper, determine its accuracy.
2nd, by transforming liquid droping port, reagent can not only be toppled over, it is also possible to which glue head dropper instills reagent.
Brief description of the drawings
Fig. 1 is the structural representation of detection culture medium in a kind of antibiotics leftover detection kit of the invention;
Fig. 2 is the structural representation of detection filter paper in a kind of antibiotics leftover detection kit of the invention;
Fig. 3 is the structural representation of the control antibiotic standard scraps of paper in a kind of antibiotics leftover detection kit of the invention.
In figure:1- inclines liquid cover, and 2- liquid droping ports, 3- detection culture mediums, 4- insertion grooves, 5- terramycin Test papers, 6- is red Mycin Test paper, 7- penicillin Test papers, 8- kanamycins Test papers, 9- detection filter papers, 10- viscosity is fixed viscous Piece, 11- yin and yang attribute detection units, the 12- control antibiotic standard scraps of paper.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made Embodiment, belongs to the scope of protection of the invention.
Fig. 1-3 are referred to, the present invention provides a kind of technical scheme:A kind of antibiotics leftover detection kit, including detection It is described to detect that use culture medium 3 includes antibiotic 3 with the control of the culture medium 3 antibiotic standard scraps of paper 12, detection filter paper 9 Culture medium dry powder, No. 4 culture medium dry powders of antibiotic, detection bacterium bag bacteria suspension containing micrococcus luteus, Bacillus subtillis Spore solution, Bacillus cereus gill fungus shape mutation spore solution, 12 points of the control antibiotic standard scraps of paper are four kinds of mycins Test paper be respectively terramycin Test paper 5, erythromycin Test paper 6, penicillin Test paper 7, kanamycins detection Test paper 8, is additionally provided with detection filter paper 9 in the kit, the left side of the detection filter paper 9 is provided with yin and yang attribute detection unit 11。
Operation principle:Trained by the way that detection filter paper 9 or the control antibiotic standard scraps of paper 12 are put into detection Support in the insertion groove in base 3, corresponding detection reagent is dripped the change that color is observed into different concentration columns from liquid droping port afterwards Change.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention And modification, the scope of the present invention be defined by the appended.

Claims (4)

1. a kind of antibiotics leftover detection kit, including detection culture medium(3), control use the antibiotic standard scraps of paper(12)With Detection filter paper(9), it is characterised in that the detection culture medium(3)Comprising No. 3 culture medium dry powders of antibiotic, antibiotic 4 Number culture medium dry powder, detection bacterium bag bacteria suspension containing micrococcus luteus, Bacillus subtillis spore solution, waxy brood cell's bar Bacterium gill fungus shape mutation spore solution, the control antibiotic standard scraps of paper(12)The Test paper for being divided into four kinds of mycins is respectively soil Mycin Test paper(5), erythromycin Test paper(6), penicillin Test paper(7), kanamycins Test paper(8), it is described Detection filter paper is additionally provided with kit(9), the detection filter paper(9)Left side is provided with yin and yang attribute detection unit(11).
2. a kind of antibiotics leftover detection kit according to claim 1, it is characterised in that:The detection culture medium (3)Surface be provided with liquid droping port(2), liquid droping port(2)Top is provided with the liquid cover that inclines(1), incline liquid cover(1)It is funnel-form, detection is used Culture medium(3)Inside is provided with test paper insertion groove(4).
3. a kind of antibiotics leftover detection kit according to claim 1, it is characterised in that:The antibiotic standard paper Piece(12)It is divided into and is divided into seven concentration cells and is tested.
4. a kind of antibiotics leftover detection kit according to claim 1, it is characterised in that:The detection filter paper (9)Left side is provided with the fixed bonding die of viscosity(10).
CN201611020680.9A 2016-11-21 2016-11-21 A kind of antibiotics leftover detection kit Pending CN106770220A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611020680.9A CN106770220A (en) 2016-11-21 2016-11-21 A kind of antibiotics leftover detection kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611020680.9A CN106770220A (en) 2016-11-21 2016-11-21 A kind of antibiotics leftover detection kit

Publications (1)

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CN106770220A true CN106770220A (en) 2017-05-31

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107607532A (en) * 2017-09-20 2018-01-19 凤阳高科动物保健品厂 A kind of method of quick detection veterinary antibiotic
CN117129471A (en) * 2023-10-27 2023-11-28 深圳市明鉴检测专业技术有限公司 Antibiotic residue detection kit and detection method thereof

Citations (4)

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Publication number Priority date Publication date Assignee Title
CN203164184U (en) * 2013-04-08 2013-08-28 季一权 Test paper insert card
CN103820310A (en) * 2014-02-15 2014-05-28 江苏中新医药有限公司 Concentration gradient detection kit for drug sensitivity tests and measuring method thereof
CN104561234A (en) * 2015-01-24 2015-04-29 福建出入境检验检疫局检验检疫技术中心 Antibiotic residue detection kit and application thereof
CN205103059U (en) * 2015-11-19 2016-03-23 李建勋 Water sample sampling bag

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN203164184U (en) * 2013-04-08 2013-08-28 季一权 Test paper insert card
CN103820310A (en) * 2014-02-15 2014-05-28 江苏中新医药有限公司 Concentration gradient detection kit for drug sensitivity tests and measuring method thereof
CN104561234A (en) * 2015-01-24 2015-04-29 福建出入境检验检疫局检验检疫技术中心 Antibiotic residue detection kit and application thereof
CN205103059U (en) * 2015-11-19 2016-03-23 李建勋 Water sample sampling bag

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Title
周庭银,倪语星等: "《临床微生物检验标准化操作》", 31 May 2015, 上海科学技术出版社 *
窦红,申宏丹等: "食品中总抗生素快速检测试剂盒性能的评价", 《动物医学进展》 *
黄晓蓉,郑晶等: "食品中多种抗生素残留的微生物筛检方法研究", 《食品科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107607532A (en) * 2017-09-20 2018-01-19 凤阳高科动物保健品厂 A kind of method of quick detection veterinary antibiotic
CN117129471A (en) * 2023-10-27 2023-11-28 深圳市明鉴检测专业技术有限公司 Antibiotic residue detection kit and detection method thereof
CN117129471B (en) * 2023-10-27 2023-12-26 深圳市明鉴检测专业技术有限公司 Antibiotic residue detection kit and detection method thereof

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Application publication date: 20170531