CN106770214A - Terbutaline detection method and detection box in a kind of poultry - Google Patents
Terbutaline detection method and detection box in a kind of poultry Download PDFInfo
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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Abstract
The invention discloses Terbutaline detection method in a kind of poultry and detection box, the detection method is comprised the following steps：S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, and poultry sample is crushed；S2, take the centrifuge tube of 1 50ml test tube and 2 50ml, be respectively labeled as pipe A, pipe B and pipe C, from S1 crush after poultry sample in take out 3 5g place with pipe A in, to adding 4 7ml concentration to be the hydrochloric acid solution of 1 1.5mol/L in pipe A and being mixed, ultrasonic wave extraction 20min is used；The invention, the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument, obtains the concentration data of Terbutaline in poultry, testing result is more directly perceived compared to other detection methods, the popularization of a large amount of selective mechanisms is suitable as, it is time saving and energy saving to have reached, cost-effective effect.
Description
Technical field
The present invention relates to technical field, Terbutaline detection method and detection box in specially a kind of poultry.
Background technology
Terbutaline belongs to β-excitant, the phenolethanolamine analog derivative of one class chemical synthesis, β-emerging during β-excitant
It is identical with adrenal gland, norepinephrine to put forth energy the agent mechanism of action, can influence nutriment flow direction in animal body and divide again
Match somebody with somebody, effectively facilitate muscular tissue growing, reduce content of fat in body, improve lean meat percentage and increase lean meat yield, but β-excitement
Agent has similar adrenergic chemical constitution, and this kind of compound is general higher as the consumption of growth accelerator, generally
5-10 times for the treatment of consumption, it turns out that such medicine is survivable, when being applied to the feed addictive of birds, if butchered
It is preceding not by the body medicine phase of certain hour, then the medicine of higher concentration can be remained in muscle and internal organs, if people is long-term
The edible poultry containing these left drugs, will produce serious toxic and side effect, such as palpitaition, headache, dizzy, Nausea and vomiting,
Tremble, neuroticism, blood vessel dilatation, increased heart rate etc., especially to hypertension, heart disease, diabetes, hyperthyroid patient harm more
Greatly, serious caused death, although China has forbidden the use of clenbuterol hydrochloride, but regularly carries out Te Bu to birds meat products
He is still a necessary monitoring means at the detection of woods residual.
At present, the method for detection Terbutaline mainly has：Microbial method, thin-layered chromatography (TLC), radioimmunology are high
Effect liquid phase chromatogram method (HPLC), color/matter combination analysis method (LC-MS), liquid/matter combination analysis method, the defect of microbial method are：
Time-consuming and shortage specificity.The defect of thin-layered chromatography is：Operating process is complicated, and the time is long；Operating personnel are needed by special
Industry training；The disturbing factor of impact analysis is more, as a result poor repeatability.Thin-layered chromatography, high performance liquid chromatography, color/matter connect
It is expensive instrument and equipment with analytic approach, the defect of liquid/matter combination analysis method these physico-chemical methods, it is time-consuming, arduously, it is not suitable as
The popularization of a large amount of selective mechanisms, testing cost is high, and the testing result for obtaining is not directly perceived.
The content of the invention
Based on the technical problem that background technology is present, the present invention proposes Terbutaline detection method and inspection in a kind of poultry
Survey box.
The present invention proposes Terbutaline detection method in a kind of poultry, and the detection method is comprised the following steps：
S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, and poultry sample is crushed；
S2, takes the centrifuge tube of 1 50ml test tube and 2 50ml, is respectively labeled as pipe A, pipe B and pipe C, the fowl after being crushed from S1
3-5g is taken out in meat sample product to place with pipe A, is the hydrochloric acid solution of 1-1.5mol/L and is carried out to addition 4-7ml concentration in pipe A
Mixing, using ultrasonic wave extraction 20min, then stands and treats its natural sedimentation, takes supernatant 2-4ml stand-by to pipe B；
S3, to adding 3-5ml concentration that supernatant pH value in pipe B is adjusted into 9.0 for the NaON solution of 8-11mol/L in pipe B, manually
Concussion 1min, centrifuge 6000r/min centrifugation 10min are put into by pipe B, take 3-5ml supernatants to stand-by in pipe C；
S4, is 1- for the wash solution and 100-300 μ L concentration of 1-1.5mol/L to 300-500 μ L concentration is added in pipe C
The calcium chloride solution of 1.5mmol/L, gently blows and beats liquid rinse pipe C inwalls, should avoid producing bubble during piping and druming, and pipe C is put into
Centrifuge, 1min is centrifuged under 4000r/min, isolates organic phase, and water phase concentration is 3-5mol/L isobutanols extraction two
It is secondary（Each 5ml）, extract is placed in 55-60 DEG C of water-bath evaporated under reduced pressure, residue is dissolved with 0.5-1mol/L cleaning solutions and is made into
1：8 solution, obtains testing sample solution；
S5, it is then a to add the μ of Terbutaline antibody-solutions 60 to two part of 60 μ L sample solution is separately added into ELISA Plate micropore
L, 2h is cultivated in 25 DEG C of environment；
S6, the liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, each
Micropore adds the μ L of luminescent solution 120, and the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument；
The luminous value of the addition Terbutaline antibody-solutions sample that will be measured is divided by no addition Terbutaline antibody-solutions sample
Luminous value be multiplied by 100% obtain sample in Terbutaline concentration.
Preferably, the supersonic frequency in the S2 selected by ultrasonic wave extraction is 40KHZ.
Preferably, the poultry after being crushed in the S1 is sterilized 2min in hydrogen peroxide.
Terbutaline detection box in a kind of poultry, the inside of the detection box is placed with ELISA Plate and reagent, wherein enzyme mark
Each micropore endoperidium of plate has antigen, and antigen is that Terbutaline and ovalbumin coupling are made, reagent include calcium chloride solution,
Luminescent solution and wash solution.
Preferably, the wash solution is KCL, 44.3mmol/L of NaOH, 2.7mmol/L containing 137mmol/L
The KH2PO of Na2HPO and 41.4mmol/L.
Preferably, the luminescent solution is to use the 0.0001mol/L p-cresols and Trisaminomethane solution that pH value is 9
Be configured to the luminol solution of 0.01mol/L, then with H2O2According to 3：10000 volume mixture.
Preferably, the Terbutaline antibody-solutions use the Anti-TNF-α obtained by the immune animal preparation of artificial immunizing antigen
Body, is diluted using wash solution and obtains 1：500 concentration.
Preferably, the concentration of the calcium chloride solution is 130mmol/L.
Beneficial effect
The invention provides Terbutaline detection method in a kind of poultry and detection box, possesses following beneficial effect：
Terbutaline detection method in the poultry, by molten to the washing for adding 300-500 μ L concentration to be 1-1.5mol/L in pipe C
Liquid and 100-300 μ L concentration are the calcium chloride solution of 1-1.5mmol/L, and pipe C is put into centrifuge, are centrifuged under 4000r/min
1min, isolates organic phase, and water phase concentration is extracted twice for 3-5mol/L isobutanols（Each 5ml）, extract is placed in
55-60 DEG C of water-bath evaporated under reduced pressure, residue is dissolved with 0.5-1mol/L cleaning solutions and is made into 1：8 solution, obtains testing sample molten
Liquid, easy to operate, testing cost also relative reduction relatively low to technical staff's level requirement, can obtain purity sample higher
Solution, the effective success rate for improving detection, it is time saving and energy saving to have reached, cost-effective effect, by the liquid in ELISA Plate micropore
Body is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, and each micropore adds the μ L of luminescent solution 120, makes
The luminous intensity of each micropore is determined with chemical illumination immunity analysis instrument, the concentration data of Terbutaline in poultry is obtained, detected
Result is more directly perceived compared to other detection methods, is suitable as the popularization of a large amount of selective mechanisms.
Specific embodiment
With reference to specific embodiment, the present invention will be further described, based on the embodiment in the present invention, this area
The every other embodiment that those of ordinary skill is obtained under the premise of creative work is not made, belongs to protection of the present invention
Scope.
The invention provides a kind of technical scheme, Terbutaline detection box in a kind of poultry detects that the inside of box is placed with
Each micropore endoperidium of ELISA Plate and reagent, wherein ELISA Plate has antigen, and antigen is Terbutaline and ovalbumin coupling system
Include calcium chloride solution, luminescent solution and wash solution into, reagent, wash solution be the NaOH containing 137mmol/L,
The KH2PO of the Na2HPO and 41.4mmol/L of KCL, 44.3mmol/L of 2.7mmol/L, it using pH value is 9 that luminescent solution is
The Trisaminomethane solution of 0.0001mol/L p-cresols is configured to the luminol solution of 0.01mol/L, then with H2O2According to 3：
The polyclonal antibody obtained by animal preparation is immunized using artificial immunizing antigen for 10000 volume mixture, Terbutaline antibody-solutions,
Diluted using wash solution and obtain 1：500 concentration, the concentration of calcium chloride solution is 130mmol/L.
Embodiment one
The invention provides a kind of technical scheme, a kind of Terbutaline detection method in poultry, detection method is comprised the following steps：
S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, poultry sample is crushed, poultry
Take out stand-by after the 2min that sterilizes is placed in hydrogen peroxide after crushing, kill the bacterium that poultry in crushing process may be infected with, it is to avoid
Its influence result of the test；
S2, takes the centrifuge tube of 1 50ml test tube and 2 50ml, is respectively labeled as pipe A, pipe B and pipe C, is not used in experimentation
Test tube and centrifuge tube to deposit stand-by in gnotobasis, from S1 crush after poultry sample in take out 3g place with pipe A
In, to adding 4ml concentration in pipe A for the hydrochloric acid solution of 1mol/L and being mixed, use ultrasonic wave extraction 20min, Ran Houjing
Put and treat its natural sedimentation, take supernatant 2ml stand-by to pipe B；
S3, to adding 3ml concentration that supernatant pH value in pipe B is adjusted into 9.0 for the NaON solution of 8mol/L in pipe B, shakes manually
1min, centrifuge 6000r/min centrifugation 10min are put into by pipe B, take 3ml supernatants to stand-by in pipe C；
S4, to the wash solution and 100 μ L concentration for adding 300 μ L concentration to be 1mol/L in pipe C for the calcium chloride of 1mmol/L is molten
Liquid, gently blows and beats liquid rinse pipe C inwalls, should avoid producing bubble during piping and druming, pipe C is put into centrifuge, under 4000r/min
Centrifugation 1min, isolates organic phase, and water phase concentration is extracted twice for 3mol/L isobutanols（Each 5ml）, extract is put
In 55-60 DEG C of water-bath evaporated under reduced pressure, residue is dissolved with 0.5mol/L cleaning solutions and is made into 1：8 solution, obtains testing sample molten
Liquid；
S5, it is then a to add the μ of Terbutaline antibody-solutions 60 to two part of 60 μ L sample solution is separately added into ELISA Plate micropore
L, 2h is cultivated in 25 DEG C of environment；
S6, the liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, each
Micropore adds the μ L of luminescent solution 120, and the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument；
The luminous value of the addition Terbutaline antibody-solutions sample that will be measured is divided by no addition Terbutaline antibody-solutions sample
Luminous value be multiplied by 100% obtain sample in Terbutaline concentration.
The embodiment one has the characteristics that compared with embodiment two and embodiment three：
The degree of accuracy it is high, it is necessary to sample size it is less.
Embodiment two
The invention provides a kind of technical scheme, a kind of Terbutaline detection method in poultry, detection method is comprised the following steps：
S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, poultry sample is crushed, poultry
Take out stand-by after the 2min that sterilizes is placed in hydrogen peroxide after crushing, kill the bacterium that poultry in crushing process may be infected with, it is to avoid
Its influence result of the test；
S2, takes the centrifuge tube of 1 50ml test tube and 2 50ml, is respectively labeled as pipe A, pipe B and pipe C, the fowl after being crushed from S1
4g is taken out in meat sample product to place with pipe A, to adding 5ml concentration in pipe A for the hydrochloric acid solution of 1.3mol/L and being mixed, is made
Ultrasonic wave extraction 20min is used, is then stood and is treated its natural sedimentation, take supernatant 3ml stand-by to pipe B；
S3, to adding 4ml concentration that supernatant pH value in pipe B is adjusted into 9.0 for the NaON solution of 9mol/L in pipe B, shakes manually
1min, centrifuge 6000r/min centrifugation 10min are put into by pipe B, take 4ml supernatants to stand-by in pipe C；
S4, is the calcium chloride of 1.2mmol/L to the wash solution and 200 μ L concentration that add 400 μ L concentration to be 1.3mol/L in pipe C
Solution, gently blows and beats liquid rinse pipe C inwalls, should avoid producing bubble during piping and druming, pipe C is put into centrifuge, in 4000r/min
Lower centrifugation 1min, isolates organic phase, and water phase concentration is extracted twice for 4mol/L isobutanols（Each 5ml）, by extract
55-60 DEG C of water-bath evaporated under reduced pressure is placed in, residue is dissolved with 0.7mol/L cleaning solutions and is made into 1：8 solution, obtains testing sample
Solution；
S5, it is then a to add the μ of Terbutaline antibody-solutions 60 to two part of 60 μ L sample solution is separately added into ELISA Plate micropore
L, 2h is cultivated in 25 DEG C of environment；
S6, the liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, each
Micropore adds the μ L of luminescent solution 120, and the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument；
The luminous value of the addition Terbutaline antibody-solutions sample that will be measured is divided by no addition Terbutaline antibody-solutions sample
Luminous value be multiplied by 100% obtain sample in Terbutaline concentration.
The embodiment two has the characteristics that compared with embodiment one and embodiment three：
The testing result stand-by period is short, and the degree of accuracy is relatively low.
Embodiment three
The invention provides a kind of technical scheme, a kind of Terbutaline detection method in poultry, detection method is comprised the following steps：
S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, poultry sample is crushed, poultry
Take out stand-by after the 2min that sterilizes is placed in hydrogen peroxide after crushing, kill the bacterium that poultry in crushing process may be infected with, it is to avoid
Its influence result of the test；
S2, takes the centrifuge tube of 1 50ml test tube and 2 50ml, is respectively labeled as pipe A, pipe B and pipe C, the fowl after being crushed from S1
5g is taken out in meat sample product to place with pipe A, to adding 7ml concentration in pipe A for the hydrochloric acid solution of 1.5mol/L and being mixed, is made
Ultrasonic wave extraction 20min is used, is then stood and is treated its natural sedimentation, take supernatant 4ml stand-by to pipe B；
S3, to adding 5ml concentration that supernatant pH value in pipe B is adjusted into 9.0 for the NaON solution of 11mol/L in pipe B, shakes manually
1min, centrifuge 6000r/min centrifugation 10min are put into by pipe B, take 5ml supernatants to stand-by in pipe C；
S4, is the calcium chloride of 1.5mmol/L to the wash solution and 300 μ L concentration that add 500 μ L concentration to be 1.5mol/L in pipe C
Solution, gently blows and beats liquid rinse pipe C inwalls, should avoid producing bubble during piping and druming, pipe C is put into centrifuge, in 4000r/min
Lower centrifugation 1min, isolates organic phase, and water phase concentration is extracted twice for 5mol/L isobutanols（Each 5ml）, by extract
55-60 DEG C of water-bath evaporated under reduced pressure is placed in, residue is dissolved with 1mol/L cleaning solutions and is made into 1：8 solution, obtains testing sample molten
Liquid；
S5, it is then a to add the μ of Terbutaline antibody-solutions 60 to two part of 60 μ L sample solution is separately added into ELISA Plate micropore
L, 2h is cultivated in 25 DEG C of environment；
S6, the liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, each
Micropore adds the μ L of luminescent solution 120, and the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument；
The luminous value of the addition Terbutaline antibody-solutions sample that will be measured is divided by no addition Terbutaline antibody-solutions sample
Luminous value be multiplied by 100% obtain sample in Terbutaline concentration.
The embodiment three has the characteristics that compared with embodiment one and embodiment two：
The testing result stand-by period is short, and the degree of accuracy is high, it is necessary to sample size is big.
In sum, Terbutaline detection method in the poultry, is 1- by 300-500 μ L concentration is added in pipe C
The wash solution and 100-300 μ L concentration of 1.5mol/L are the calcium chloride solution of 1-1.5mmol/L, and pipe C is put into centrifuge,
1min is centrifuged under 4000r/min, organic phase is isolated, water phase concentration is extracted twice for 3-5mol/L isobutanols（Every time
5ml）, extract is placed in 55-60。C water-bath evaporated under reduced pressure, residue is dissolved with 0.5-1mol/L cleaning solutions and is made into 1：8 it is molten
Liquid, obtains testing sample solution, easy to operate, relatively low to technical staff's level requirement, testing cost also relative reduction, can obtain
To purity sample solution higher, the effective success rate for improving detection, it is time saving and energy saving to have reached, cost-effective effect, will
Liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, and each micropore is added
The μ L of luminescent solution 120, the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument, obtains Terbutaline in poultry
Concentration data, testing result compared to other detection methods it is more directly perceived, be suitable as the popularization of a large amount of selective mechanisms.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposited between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant be intended to
Nonexcludability is included, so that process, method, article or equipment including a series of key elements not only will including those
Element, but also other key elements including being not expressly set out, or also include being this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions.By sentence " including one ... the key element for limiting, it is not excluded that
Also there is other identical element in the process including the key element, method, article or equipment ".
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (8)
1. Terbutaline detection method in a kind of poultry, it is characterised in that the detection method is comprised the following steps：
S1, takes fresh poultry, and the fat wiped out in poultry sample obtains musculature, and poultry sample is crushed；
S2, takes the centrifuge tube of 1 50ml test tube and 2 50ml, is respectively labeled as pipe A, pipe B and pipe C, the fowl after being crushed from S1
3-5g is taken out in meat sample product to place with pipe A, is the hydrochloric acid solution of 1-1.5mol/L and is carried out to addition 4-7ml concentration in pipe A
Mixing, using ultrasonic wave extraction 20min, then stands and treats its natural sedimentation, takes supernatant 2-4ml stand-by to pipe B；
S3, to adding 3-5ml concentration that supernatant pH value in pipe B is adjusted into 9.0 for the NaON solution of 8-11mol/L in pipe B, manually
Concussion 1min, centrifuge 6000r/min centrifugation 10min are put into by pipe B, take 3-5ml supernatants to stand-by in pipe C；
S4, is 1- for the wash solution and 100-300 μ L concentration of 1-1.5mol/L to 300-500 μ L concentration is added in pipe C
The calcium chloride solution of 1.5mmol/L, gently blows and beats liquid rinse pipe C inwalls, should avoid producing bubble during piping and druming, and pipe C is put into
Centrifuge, 1min is centrifuged under 4000r/min, isolates organic phase, and water phase concentration is 3-5mol/L isobutanols extraction two
It is secondary（Each 5ml）, extract is placed in 55-60 DEG C of water-bath evaporated under reduced pressure, residue is dissolved with 0.5-1mol/L cleaning solutions and is made into
1：8 solution, obtains testing sample solution；
S5, it is then a to add the μ of Terbutaline antibody-solutions 60 to two part of 60 μ L sample solution is separately added into ELISA Plate micropore
L, 2h is cultivated in 25 DEG C of environment；
S6, the liquid in ELISA Plate micropore is poured out, and the wash solution of 260 μ L is added in each micropore, is patted dry after washing, each
Micropore adds the μ L of luminescent solution 120, and the luminous intensity of each micropore is determined using chemical illumination immunity analysis instrument；By adding for measuring
The luminous value for entering Terbutaline antibody-solutions sample is being multiplied by divided by the luminous value of no addition Terbutaline antibody-solutions sample
100% obtains Terbutaline concentration in sample.
2. Terbutaline detection method in a kind of poultry according to claim 1, it is characterised in that：Ultrasonic wave in the S2
It is 40KHZ to extract selected supersonic frequency.
3. Terbutaline detection method in a kind of poultry according to claim 1, it is characterised in that：After being crushed in the S1
Poultry sterilized in hydrogen peroxide 2min.
4. Terbutaline detects box in a kind of poultry, it is characterised in that：The inside of the detection box is placed with ELISA Plate and reagent,
Each micropore endoperidium of wherein ELISA Plate has antigen, and antigen is that Terbutaline and ovalbumin coupling are made, and reagent includes chlorine
Change calcium solution, luminescent solution and wash solution.
5. Terbutaline detects box in a kind of poultry according to claim 4, it is characterised in that：The wash solution be containing
There is the KH2PO of the Na2HPO and 41.4mmol/L of KCL, 44.3mmol/L of NaOH, 2.7mmol/L of 137mmol/L.
6. Terbutaline detects box in a kind of poultry according to claim 4, it is characterised in that：The luminescent solution is to make
The luminol solution of 0.01mol/L is configured to the 0.0001mol/L p-cresols and Trisaminomethane solution that pH value is 9, then
With H2O2According to 3：10000 volume mixture.
7. Terbutaline detects box in a kind of poultry according to claim 4, it is characterised in that：The Terbutaline antibody
Solution is diluted using wash solution and is obtained 1 using the polyclonal antibody obtained by the immune animal preparation of artificial immunizing antigen：500
Concentration.
8. Terbutaline detects box in a kind of poultry according to claim 4, it is characterised in that：The calcium chloride solution
Concentration is 130mmol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611112525.XA CN106770214A (en) | 2016-12-07 | 2016-12-07 | Terbutaline detection method and detection box in a kind of poultry |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611112525.XA CN106770214A (en) | 2016-12-07 | 2016-12-07 | Terbutaline detection method and detection box in a kind of poultry |
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| CN106770214A true CN106770214A (en) | 2017-05-31 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078725A (en) * | 2007-06-28 | 2007-11-28 | 山东大学 | Terbutaline conjugate and its preparation method and uses |
| CN101393210A (en) * | 2008-10-24 | 2009-03-25 | 山东大学 | Chemiluminescence ELISA detection kit of terbutaline |
| CN103592434A (en) * | 2013-03-21 | 2014-02-19 | 杭州迪恩科技有限公司 | One-step method enzyme-linked immunosorbent assay method and kit for detection of beta-excitants |
| CN104849377A (en) * | 2015-01-29 | 2015-08-19 | 衢州市质量技术监督检测中心 | Pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography/mass spectrometry |
-
2016
- 2016-12-07 CN CN201611112525.XA patent/CN106770214A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078725A (en) * | 2007-06-28 | 2007-11-28 | 山东大学 | Terbutaline conjugate and its preparation method and uses |
| CN101393210A (en) * | 2008-10-24 | 2009-03-25 | 山东大学 | Chemiluminescence ELISA detection kit of terbutaline |
| CN103592434A (en) * | 2013-03-21 | 2014-02-19 | 杭州迪恩科技有限公司 | One-step method enzyme-linked immunosorbent assay method and kit for detection of beta-excitants |
| CN104849377A (en) * | 2015-01-29 | 2015-08-19 | 衢州市质量技术监督检测中心 | Pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography/mass spectrometry |
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