CN106754863A - Improve the dry processing method of xylanase activity - Google Patents
Improve the dry processing method of xylanase activity Download PDFInfo
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- CN106754863A CN106754863A CN201611219464.7A CN201611219464A CN106754863A CN 106754863 A CN106754863 A CN 106754863A CN 201611219464 A CN201611219464 A CN 201611219464A CN 106754863 A CN106754863 A CN 106754863A
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
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Abstract
The present invention relates to a kind of dry processing method for improving xylanase activity, comprise the following steps：Trestle table is set in the processing chamber housing of apparatus for low-temperature plasma treatment, and trestle table is provided with porous material, and zytase is put on the porous material；Processing chamber housing is emptied, working gas is passed through, and the 12min of discharge process 2, wherein gas pressure intensity are 20 95Pa, discharge power is 50 300W.The method of the present invention can effectively improve the activity of zytase, improve its application effect in fields such as weavings, and Low Temperature Plasma Treating process belongs to dry state treatment again, do not use chemicals, environmental protection, energy-saving and emission-reduction, with boundless market prospects.
Description
Technical field
The present invention relates to enzyme modified working process technical field, more particularly to a kind of improve the dry of xylanase activity
State processing method.
Background technology
Zytase is can be by xylan degrading into xylo-oligosaccharide and the hydrolase of xylose.One xylan molecule it is complete
Enzymolysis needs a few step enzymatic reactions, wherein the enzyme for acting on main chain has two kinds：β-Isosorbide-5-Nitrae-zytase and xylobiase.Typically
For,, from main chain internal action in xylose glycosidic bond, by xylanolitic into xylo-oligosaccharide, and the latter then acts on oligomeric wood for the former
Sugar end and discharge xylose.
Zytase is widely used the fields such as weaving, food, papermaking, the energy, feed and bioconversion, such as,
Zytase is used as biological degumming enzyme, and the colloid in energy enzyme degradation of ramie is decomposed into water-soluble small-molecule substance, is divided
From fibre bundle；Zytase reduces bleaching chlorine as new association with pulp bleaching auxiliary agent, and the environment solved in pulp industry is dirty
Dye problem；Zytase is used as feed enzyme preparation, and livestock and poultry can be promoted to digest and assimilate roughage；In xylanase hydrolysis plant
Hemicellulose, the pentose of formation is further used for the products such as production xylitol, alcohol, organic acid, biological in renewable resource
Play an important roll in trans-utilization.
Early in the sixties, people just have started to the research to zytase, and are separated to from the microorganism of separate sources big
The different type zytase of different nature of amount.In use, typically require zytase have catalytic efficiency higher, preferably
Resistance, wider pH and Acclimation temperature scope etc..In recent years, it is a large amount of continuous with the research of function about zytase structure
Launch, on the one hand deepened the understanding of the molecule mechanism to zytase effect, be on the other hand by genetic engineering and albumen
Matter engineering improves the property of zytase, develops the zytase product for meeting the requirement of different application field there is provided guidance.
Low temperature plasma is the state of material the 4th after solid-state, liquid, gaseous state, when applied voltage reach gas
When thermoelectricity is pressed, gas molecule is breakdown, produces including electronics, ion, atom and mixture etc. free radical.Low temperature plasma belongs to
In the upper state for exciting, ionizing, the negative electrical charge of its electronics and the positive charge sum of ion are equal, do not show electricity externally macroscopically
Property, in neutrality.Although discharge of plasma in low temperature process electron temperature is very high, heavy particle temperature is very low, and whole system is presented
Low-temperature condition, so referred to as low temperature plasma, is also nonequilibrium condition plasma.
The content of the invention
In order to solve the above technical problems, it is an object of the invention to provide a kind of dry state treatment side for improving xylanase activity
Method, the method that the present invention is provided can effectively improve the activity of zytase, improve its application effect in fields such as weavings,
And the method belongs to dry processing method again, chemicals, environmental protection, energy-saving and emission-reduction, with boundless city are not used
Field prospect.
A kind of dry processing method for improving xylanase activity of the invention, comprises the following steps：
(1) about one penetrating horizontal stand platform, support are set in the processing chamber housing of apparatus for low-temperature plasma treatment
Platform is provided with the porous material in thin, planar shape, and zytase is put on the porous material；
(2) processing chamber housing is emptied, is passed through working gas, and discharge process 2-10min, wherein gas pressure intensity are
20-95Pa, discharge power is 50-250W.
Further, in step (1), porous material is perforated web or porous membrane.
Further, in step (1), the material of porous material is macromolecular fibre or metallic fiber.Macromolecular fibre
It is organic polymer fiber or inorganic polymer fiber.
Further, in step (1), zytase is solid-state zytase.
Further, in step (1), zytase thinly, is equably laid on the porous material.
Further, in step (1), in step (2), discharge type is glow discharge.
Further, in step (2), working gas elder generation gas washing 2-3 times is used.After gas washing terminates, intake valve is opened, treated
After steady air current, electric discharge device is opened.
Further, in step (2), working gas is one or more in oxygen, nitrogen and argon gas.
Further, in step (2), when working gas is oxygen, gas pressure intensity is 50-95Pa, and discharge power is
100-200W, the discharge process time is 2-6min.
Further, in step (2), when working gas is nitrogen, gas pressure intensity is 50-65Pa, and discharge power is
100-200W, the discharge process time is 6-8min.
Further, in step (2), when working gas is argon gas, gas pressure intensity is 20-95Pa, and discharge power is
100-250W, the discharge process time is 2-10min.
Further, it is further comprising the steps of after step (2)：
Collection step (2) treatment after zytase, by it at 2-8 DEG C sealing preserve.
The present invention is entered using conditions such as different experiments atmosphere, gas pressure intensity, process time and discharge powers to zytase
Row low temperature plasma dry state treatment, using serial energetic ion, electronics isoreactivity material and xylan in low temperature plasma
Enzyme effect, is capable of achieving to modify the macromolecular of zytase and its Lian Shang functional groups, or even can play the generation such as space conformation
Change, from its activity and stability can be caused to be obviously improved, be conducive to further developing zytase in fields such as weavings
Application potential.
By such scheme, the present invention at least has advantages below：
1st, the invention provides a kind of Low Temperature Plasma Treating method for improving xylanase activity and stability, pass through
Contrast low temperature plasma before processing after xylanase activity, find Low Temperature Plasma Treating after xylanase activity and
Stability is significantly improved；
2nd, the inventive method is the modified working process to standard biologic enzyme, corona treatment cycle is short, it is simple to operate,
Convenient and swift, processing cost is low；
3rd, the inventive method belongs to dry state treatment, and use chemicals useless is environmentally safe, efficiently avoid biography
The chemicals consumption that is brought of system chemical Degumming is big, and energy consumption water consume is high, the problems such as environmental pollution is serious.
Brief description of the drawings
Fig. 1 is the standard curve of xylanase activity test of the present invention；
Fig. 2 is xylanase treatment result figure in the embodiment of the present invention 1,2,3；
Fig. 3 is xylanase treatment result figure in the embodiment of the present invention 4,5,6；
Fig. 4 is xylanase treatment result figure in the embodiment of the present invention 7,8,9.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the invention is described in further detail.Hereinafter implement
Example is not limited to the scope of the present invention for illustrating the present invention.
The reagent and its compound method that the present invention is used are as follows：
DNS reagents：Weigh 3,5- dinitrosalicylic acids 3.15g and be dissolved in 500mL water, 45 DEG C of water-bath is placed in after stirring 5s
In.The sodium hydroxide solution of 100mL 0.2g/mL is then gradually adding, while being stirred continuously, it is ensured that temperature is not higher than 48 DEG C, directly
It is as clear as crystal to solution.Rochelle salt 91.0g, phenol 2.50g and anhydrous sodium sulfite 2.50g are gradually added again.After
Continue 45 DEG C of heating water baths, while adding water 300mL, be stirred continuously, until the material for adding is completely dissolved.Stop heating, cooling
To room temperature, add water constant volume 1000mL.Filtered with fritted glass filter.Filtrate is taken, is stored in brown bottle, kept in dark place.
Can be used after depositing 7 days at room temperature, the term of validity is 6 months.
Buffer solution (pH=4.8,0.1mol/L)：Take 0.1mol/L citric acid solutions 460mL and 0.1mol/L sodium citrates
Solution 540mL is well mixed, and adjusts pH value to 4.8 standby.
D- xylose solutions (0.5mg/mL)：Weigh and the D- xylose 0.05000g until constant weight are dried at 105 DEG C, be accurate to
0.0001g, with the citric acid-trisodium citrate buffer solution of pH=4.8, is settled to 100mL.
Xylan solution (1mg/mL)：Weigh xylan 0.1000g, be accurate to 0.0001g, with the citric acid of pH=4.8-
Trisodium citrate buffer solution, is settled to 100mL.
Fig. 1 is the standard curve of xylanase activity test of the present invention, and its preparation method is as follows：
7 25mL color-comparison tube number consecutivelies are taken, corresponding 0.5mg/mL D- xyloses and buffering are separately added into by table 1
Liquid, is well mixed, with D- xylose solution concentration be followed successively by 0.00,0.05,0.10,0.15,0.20,0.25,0.30,
0.35mg/mL.DNS reagent 3mL are separately added into, are well mixed, be put into accurate response 5min in boiling water bath, immediately with cold after taking-up
Water cooling, plus deionized water constant volume 25mL, shake up.It is blank, school baseline, with the cuvette of lcm in 200- with No. 1 test tube
No. 6 test tubes of METHOD FOR CONTINUOUS DETERMINATION in the range of 800nm, determine that maximum absorption wavelength λ max are 492nm.It is again blank with No. 1 test tube, successively
Absorbance of other test tubes at 492nm is determined, and records each sample absorbance for measuring.Sat so that D- Xylose Contents C is vertical
Mark, corresponding absorbance A is abscissa, sets up standard curve.Table 1 is each solution used when each sample liquid of standard curve is prepared
Volume.
Each sample liquid of the standard curve of table 1 is prepared
Embodiment 1
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using argon gas reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From argon gas as working gas, condition of work is for this implementation：Gas pressure intensity 20Pa, discharge power
100W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Enzymatic activity is carried out using following methods and enzymatic activity retention rate is tested：
Xylanase solution (1.0mg/mL)：The zytase 0.05000g after above method treatment is weighed, is accurate to
0.0001g, with the citric acid-trisodium citrate buffer solution of pH=4.8, is settled to 50mL.
1mg/mL xylan solution 1mL are added in color-comparison tube, are put into 50 DEG C of thermostat water baths and are balanced 10min,
Same 1.0mg/mL xylanase solution 1mL equilibrated in 50 DEG C of water-baths are added, is shaken up, while stopwatch gets started
Timing.Colorimetric cylinder is taken out after accurate reaction 10min, 3mLDNS reagents are rapidly joined, is put into boiling water to boil at once after shaking up and is boiled
5min.Cooled down with cold water after taking-up, plus deionized water constant volume 25mL, shake up.Zytase in said process is replaced by slow
Fliud flushing, blank sample is prepared using same method.2mL buffer solutions and 3mLDNS boilings are boiled into the solution after 5min constant volumes 25mL for base
Line, determines the absorbance that sample and blank sample are tested at 492nm.
Enzymatic activity is calculated as follows：
Enzymatic activity is defined：Under the conditions of 50 DEG C, pH are for 4.8,1g zytase 1min hydrolyzed xylans generation 1ugD- xyloses
It is 1 xylanase activity unit of force, is represented with U/g.Simultaneously according to the fit equation of above-mentioned experiment gained standard working curve, its
The computing formula of catalysis activity is as follows：
In formula, X represents the xylanase activity in sample, U/g；AEIt is the absorbance of enzymatic hydrolysis reaction liquid in test sample；AB
It is the absorbance of correspondence blank sample；K and b are respectively the slope and intercept of standard curve；V is the volume of enzyme digestion reaction liquid, unit
ml；T is the zytase catalytic hydrolysis reaction time, and unit is min；The zytase quality added in m reactions, unit g.
After Low Temperature Plasma Treating, zytase catalysis activity retention rate R can be calculated according to equation below：
In formula, R is enzymatic activity retention rate, X0And X1Refer to the activity of zytase before and after Low Temperature Plasma Treating respectively, it is single
Position U/g.
Use the above method measure before Low Temperature Plasma Treating xylanase activity for 20549.01U/g the present embodiment
Treated xylanase activity is 21741.06U/g, and enzymatic activity retention rate is 105.80%.Result is as shown in a in Fig. 2.Can
To find out that enzymatic activity retention rate is more than 100% under the treatment conditions of embodiment 1, illustrate that the enzymatic activity of zytase is better than before processing
Enzymatic activity, the activity of enzyme is improved, and enzymatic activity is improved.
Embodiment 2
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using nitrogen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From nitrogen as working gas, condition of work is for this implementation：Gas pressure intensity 50Pa, discharge power
100W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 2
Shown in b.Result shows that the present embodiment xylanase activity is 22072.19U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 109.17%.As can be seen that the enzymatic activity of zytase and enzymatic activity retention rate be all under the treatment conditions of the present embodiment
It is significantly improved.
Embodiment 3
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using oxygen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From oxygen as working gas, condition of work is for this implementation：Gas pressure intensity 95Pa, discharge power
100W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 2
Shown in c.Result shows that the present embodiment xylanase activity is 21608.61U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 105.16%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the treatment conditions of the present embodiment
Raising is arrived.
Embodiment 4
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using oxygen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From oxygen as working gas, condition of work is for this implementation：Gas pressure intensity 65Pa, discharge power
100W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 3
Shown in a.Result shows that the present embodiment xylanase activity is 21145.03U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 102.90%.As can be seen that the enzymatic activity of zytase and enzymatic activity retention rate be all under the treatment conditions of the present embodiment
It is improved.
Embodiment 5
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using argon gas reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From argon gas as working gas, condition of work is for this implementation：Gas pressure intensity 80Pa, discharge power
150W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 3
Shown in b.Result shows that the present embodiment xylanase activity is 21211.26U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 103.22%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the treatment conditions of the present embodiment
Raising is arrived.
Embodiment 6
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using oxygen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From oxygen as working gas, condition of work is for this implementation：Gas pressure intensity 80Pa, discharge power
200W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 3
Shown in c.Result shows that the present embodiment xylanase activity is 21409.93U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 104.19%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the present embodiment treatment conditions
Improve.
Embodiment 7
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using oxygen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From oxygen as working gas, condition of work is for this implementation：Gas pressure intensity 65Pa, discharge power
200W, process time 2min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 4
Shown in a.Result shows that the present embodiment xylanase activity is 21741.06U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 105.80%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the treatment conditions of the present embodiment
Raising is arrived.
Embodiment 8
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using argon gas reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From argon gas as working gas, condition of work is for this implementation：Gas pressure intensity 80Pa, discharge power
200W, process time 4min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 4
Shown in a.Result shows that the present embodiment xylanase activity is 21873.51U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 106.45%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the present embodiment treatment conditions
Improve.
Embodiment 9
Zytase 0.200g is weighed, about one penetrating horizontal stand platform is set in low-temperature plasma cavity, at this
On trestle table set one launch thin, planar perforated web, then by zytase thinly, equably carry out dispersed placement,
It is then shut off the cavity door of apparatus for low-temperature plasma treatment.
Above-mentioned cavity is emptied using the vavuum pump of system configuration, and using nitrogen reaction cavity is carried out gas washing or
Gas displacement 2-3 times.From nitrogen as working gas, condition of work is for this implementation：Gas pressure intensity 50Pa, discharge power
150W, process time 8min.After gas scrubbing terminates, intake valve is opened, after pending atmosphere pressure and steady air current, unlatching is put
Electric switch carries out glow discharge, and countdown is started while opening discharge power.After the completion for the treatment of, discharge switch, vacuum are closed
Valve, is then shut off intake valve, after question response cavity air pressure returns to atmospheric pressure, opens cavity door, and collects the wood after treatment and gather
Carbohydrase, the sealing preserve at 2-8 DEG C.
Tested using the enzymatic activity before and after the method test processes in embodiment 1 and enzymatic activity retention rate, as a result as in Fig. 4
Shown in a.Result shows that the present embodiment xylanase activity is 21476.16U/g after Low Temperature Plasma Treating, and enzymatic activity retains
Rate is 104.51%.It can be seen that the enzymatic activity of zytase and enzymatic activity retention rate are all obtained under the treatment conditions of the present embodiment
Raising is arrived.
The above is only the preferred embodiment of the present invention, is not intended to limit the invention, it is noted that for this skill
For the those of ordinary skill in art field, on the premise of the technology of the present invention principle is not departed from, can also make it is some improvement and
Modification, these are improved and modification also should be regarded as protection scope of the present invention.
Claims (9)
1. it is a kind of improve xylanase activity dry processing method, it is characterised in that comprise the following steps：
(1) trestle table is set in the processing chamber housing of apparatus for low-temperature plasma treatment, and the trestle table is provided with porous material,
Zytase is placed on the porous material；
(2) processing chamber housing is emptied, is passed through working gas, and discharge process 2-12min, wherein gas pressure intensity are
20-95Pa, discharge power is 50-300W.
2. it is according to claim 1 improve xylanase activity dry processing method, it is characterised in that：In step (1)
In, the porous material is perforated web or porous membrane.
3. according to claim 1 or 3 raising xylanase activity dry processing method, it is characterised in that：In step
(1) in, the material of the porous material is macromolecular fibre or metallic fiber.
4. it is according to claim 1 improve xylanase activity dry processing method, it is characterised in that：In step (1)
In, the zytase is solid-state zytase.
5. it is according to claim 1 improve xylanase activity dry processing method, it is characterised in that：In step (1)
In, in step (2), discharge type is glow discharge.
6. it is according to claim 1 improve xylanase activity dry processing method, it is characterised in that：In step (2)
In, the working gas is one or more in oxygen, nitrogen and argon gas.
7. it is according to claim 6 improve xylanase activity dry processing method, it is characterised in that：In step (2)
In, when the working gas is oxygen, gas pressure intensity is 50-95Pa, and discharge power is 100-200W, and the discharge process time is 2-
6min。
8. it is according to claim 6 improve xylanase activity dry processing method, it is characterised in that：In step (2)
In, when the working gas is nitrogen, gas pressure intensity is 50-65Pa, and discharge power is 100-200W, and the discharge process time is 6-
8min。
9. it is according to claim 6 improve xylanase activity dry processing method, it is characterised in that：In step (2)
In, when the working gas is argon gas, gas pressure intensity is 20-95Pa, and discharge power is 100-250W, and the discharge process time is 2-
10min。
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| CN101701212A (en) * | 2009-11-13 | 2010-05-05 | 清华大学 | Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma |
| CN106191083A (en) * | 2016-07-19 | 2016-12-07 | 湖北大学 | Xylanase mutant that a kind of specific enzyme activity improves and encoding gene and application |
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| CN101701212A (en) * | 2009-11-13 | 2010-05-05 | 清华大学 | Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma |
| CN106191083A (en) * | 2016-07-19 | 2016-12-07 | 湖北大学 | Xylanase mutant that a kind of specific enzyme activity improves and encoding gene and application |
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