CN106754567A - A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS for efficiently preventing and treating various crop droop - Google Patents
A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS for efficiently preventing and treating various crop droop Download PDFInfo
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Abstract
The invention discloses a kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS for efficiently preventing and treating various crop droop, it is made up of bacillus JC65, Bacillus cercus CH01, Bacillus subtillis SM16.By 3 kinds of bacterium single doses according to each bacterium number of viable 1:1:1 is made into mix preparation, and viable bacteria total concentration is 1 × 10 in mixture8~1010CFU/ml.LAS mixture is to the prevention effect of crop droop up to more than 70%.To the growth-promoting effect of disease-free plant up to 20%, the composite bacteria agent needs to carry out sowing and transplant two links.
Description
Technical field
The present invention relates to a kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS for efficiently preventing and treating various crop droop, belong to agro-ecology and prevent
Control technical field.
Background technology
Occupy very important status in watermelon, cucumber, muskmelon and pumpkin agricultural crops yield in the world, its
Middle wilt disease is one of influence 4 kinds of Major Diseases of agricultural crops yield and quality of appeal.Droop is by Fusarium oxysporum
(Fusarium oxysporum f.sp.) causes a kind of destructive soil-borne disease, is commonly called as stem wilt, dead seedling disease etc., alive
There is generation in the range of boundary.The generation of droop has more been aggravated with the expansion of cultivated area and continuous cropping plantation.
At present, wilt disease is prevented and treated main measure and still uses chemical agent.Although chemical agent can be in certain journey
Droop is prevented and treated on degree, but at the same time, many beneficial microorganisms can also be subject to different degrees of injury in soil.Some
The use of chemical agent can cause the serious problem such as soil pollution and chemical pesticide residual.In addition, breeding resistant variety
Controlling disease is also had certain effect with enhancement of field management measure.But the seed selection cycle of disease-resistant variety is long, and droop
Bacterium Variation of Virulence is big, and resistance easily disappears foster.
Relatively effective in agricultural measures is crop rotation, and especially rice field-upland field rotation can effectively reduce bacterium in soil
Amount, reduces disease and occurs, but there is certain difficulty in actual applications.One is that watermelon, cucumber, pumpkin and muskmelon belong to high economical
Value crop；Two is that short ratation does not reach expected purpose because Fusarium oxysporum bacterium can survive for more than ten to decades in soil；Three
That the most of non-host plants of plantation can not reduce quantity of the Microsclerotia in soil, and non-host root secretion remain to for
Microsclerotia survival provides nutrition.
Under this development, many sight begin to transfer to biological control means.Biological control and other method phase
Than, with safely, effectively, it is lasting the characteristics of, be especially that of avoiding a series of problems that chemical prevention brings.Biological prevention and control agent is to disease
Evil prevention effect is good, free from environmental pollution, noresidue nontoxic to people and animals；To the killing high specificity of pest and disease damage, natural enemy is not injured,
Beneficial organism, can keep ecological balance；Raw materials for production and active ingredient are that natural products is degradable, can be gone back to nature, it is ensured that
Sustainable development；Microorganism can be changed with the method for biotechnology and genetic engineering；Many factors and composition play a role,
Pathogen is difficult to develop immunity to drugs.It is reported that the biocontrol fungi of droop mainly has Trichoderma, viscous broom mould, but above-mentioned biocontrol microorganisms
Prevention effect is not ideal enough, and main cause is these biocontrol microorganisms poor in host's rhizospere competition.Compare in droop biocontrol bacteria
More promising species is bacillus, bacillus subtilis, Pseudomonas fluorescens, bacillus etc..It is, in general, that various lifes
It is fungi-proofing that to be used in mixed way its effect better than single biocontrol microorganisms.In addition, biocontrol agent and agricultural cultivation measure are used in combination with,
A good border of dwelling can be provided for biocontrol microorganisms, the preventing and treating potential of itself can be played as far as possible.
The content of the invention
The microbial composite of various crop droop and prepared therefrom is efficiently prevented and treated it is an object of the present invention to provide a kind of
Complex microbial inoculum.
It is a further object of the present invention to provide the application of the microbial composite and complex microbial inoculum prepared therefrom.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of microbial composite for efficiently preventing and treating various crop droop, is made up of following 3 kinds of bacteriums, is respectively：Bud
Born of the same parents bacillus JC65 (Bacillus sp.), was preserved in China Committee for Culture Collection of Microorganisms general on December 15th, 2010
Logical microorganism center, culture presevation number is CGMCC No.4475；Bacillus cercus CH01 (Bacillus cereuse), in
On November 17th, 2015 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and culture presevation number is
CGMCC NO.11677；Bacillus subtillis SM16 is (Bacillus subtilis), in being preserved on 06 18th, 2009
State's Microbiological Culture Collection administration committee common micro-organisms center, culture presevation number is CGMCC No.3127.
Application of the microbial composite of the present invention in terms of preventing and treating droop and promoting plant growth.
It is preferred that the preservative fluid for thallus of the wet bacterium and this laboratory after described three kinds of bacterial strains JC65, CH01 and SM16 are fermented
(belong to this laboratory granted patent, patent No. ZL02112559.7, publication CN1358838, publication date：In July, 2002
17 days) press 1：40 (g/ml) are made into microbial inoculum, can room temperature preservation 2~3 years.The composite bacteria agent can be diluted when watermelon transplantation of seedlings
Pour and apply.
Microbial composite of the present invention prevents and treats the microbial composite bacteria of droop and promotion plant growth preparing
Application in agent.
Complex microbial inoculum prepared by microbial composite of the present invention.
Complex microbial inoculum of the present invention, is preferably prepared by the following method and obtains：By described bacterial strain JC65,
CH01 and SM16 are inoculated into the flat lining outs of LB respectively, and 14-16h is cultivated in 28 DEG C of biochemical cultivation cases, after single bacterium colony is grown, choose
Take in the test tube that single bacterium colony accesses the LB nutrient solutions containing 5mL, be placed in 28 DEG C, cultivated in the shaking table of 200r/min, be made seed
Liquid；Seed liquor is inoculated in the triangular flask of the LB nutrient solutions equipped with 500mL with 1% inoculum concentration, is placed in 28 DEG C, 200r/min
Shaking table in cultivate 48h, 3 kinds of bacterial concentrations are diluted to 5 × 107According to 1 after CFU/mL:1:1 ratio is well mixed, as
Described complex microbial inoculum.
The preparation method of complex microbial inoculum of the present invention, including：By described bacterial strain JC65, CH01 and SM16
The flat lining outs of LB being inoculated into respectively, 14-16h being cultivated in 28 DEG C of biochemical cultivation cases, after single bacterium colony is grown, picking single bacterium colony connects
Enter in the test tube of the LB nutrient solutions containing 5mL, be placed in 28 DEG C, cultivated in the shaking table of 200r/min, be made seed liquor；With 1%
Be inoculated in seed liquor in the triangular flask of the LB nutrient solutions equipped with 500mL by inoculum concentration, is placed in 28 DEG C, is trained in the shaking table of 200r/min
48h is supported, 3 kinds of bacterial concentrations are diluted to 5 × 107According to 1 after CFU/mL:1:1 ratio is well mixed.
Application of the complex microbial inoculum of the present invention in terms of preventing and treating droop and promoting plant growth.
Described application is preferably diluted pouring root and uses when crop is transplanted and sows.
Beneficial effect
Advantages and positive effects of the present invention show：The present invention is specific to the biological and ecological methods to prevent plant disease, pests, and erosion system of droop disease exploitation
Agent.Because it belongs to biological agent, do not use brought a series of problems because of chemical pesticide completely, beneficial to watermelon, cucumber,
The No-harmful apple orchard of pumpkin and muskmelon, peasant can without or reduce the consumption of other chemical pesticides, this can not only be saved for peasant
Spending is saved, and is conducive to the export trade of these crops.
Greenhouse test shows that LAS mixture can effectively control droop to infect watermelon, reaches the effect of symptom management generation
Really, protection effect has 86.7%, and growth-promoting effect has 33.3%；Field experiment shows that LAS mixture on the one hand can symptom management hair
Raw, preventive effect is maintained at 75.0%, while 4 kinds of growths of crop can be promoted to appeal, increasing both production and income, and growth-promoting effect is up to 66.6%；
Comprehensive greenhouse and the experiment of crop field growth-promoting find that LAS mixture can promote to appeal 4 kinds of growths of crop, improve yield.
Brief description of the drawings
The antagonistic effect figure of Fig. 1 difference biocontrol microorganisms and wilt
The suppression figure that Fig. 2 difference biocontrol microorganisms grow to wilt
The growth-promoting design sketch of 30 days after the transplanting of Fig. 3 watermelons
Biomaterial preservation information
JC65, bacillus Bacillus sp., Chinese microorganism strain preservation is preserved on December 15th, 2010
Administration committee's common micro-organisms center, preservation address is the institute of BeiChen West Road, Chaoyang District, BeiJing City No. 13, and the Chinese Academy of Sciences is micro-
Biological study institute, culture presevation number is CGMCC No.4475.
CH01, Bacillus cercus Bacillus cereuse, China Microbiological is preserved on November 17th, 2015
Culture presevation administration committee common micro-organisms center, preservation address is the institute of BeiChen West Road, Chaoyang District, BeiJing City No. 13, China
Institute of microbiology of the academy of sciences, culture presevation number is CGMCC NO.11677.
SM16, Bacillus subtillis Bacillus subtilis, Chinese micro- life was preserved on 06 18th, 2009
Thing culture presevation administration committee common micro-organisms center, preservation address is the institute of BeiChen West Road, Chaoyang District, BeiJing City No. 13, in
Institute of microbiology of the academy of sciences of state, culture presevation number is CGMCC No.3127.
Specific embodiment
Embodiment 1
1. bacterium source：JC65 is by isolated in Cucumber rhizosphere soil；SM16 and CH01 is by tomato healthy tree root soil
In it is isolated.
2. strain separation method：It is (micro- with reference to microbial room of Chinese Academy of Sciences Nanjing soil institute soil using plate dilution method
Biological study method [M] Beijing：Scientific publication is shut out, and 1985.).
3. bacterial strain screening foundation：Watermelon blight germ determines (opposite culture method, Lin Fu with the antagonism of biocontrol microorganisms
It is in, bacteriolysis [J] the plant pathologies of Li De forts bacillus subtilises (Bacillus subtilis) to plant pathogenic fungi
Journal, 2003,33 (2)：174~177.), biocontrol microorganisms are prevented and treated watermelon blight and growth-promoting functions greenhouse, field experiment.
4. identification of strains method：Identified using prokaryotes 16S rRNA encoding gene segment sequences method.Identification
The results are shown in Table 1.
Table 1：Sequencing comparison result
5. flat board antagonistic experiment method
Method one：The withered germ of water-melon that will be stored in 4 DEG C is inoculated on PDA plate and activates, and treats that fungi covers with flat board
Uniformly break into the circular bacterium block of a diameter of 8mm from bacterium colony outward flange with the card punch of sterilizing afterwards.By inoculated by hypha block new
The center of PDA plate, is inoculated with one plant of biocontrol microorganisms, as shown in Figure 1 respectively in its surrounding away from center equidistant.25 DEG C of cultures, often
Fungi growing state was observed every two days, the size of inhibition zone is recorded.Each three repetition for the treatment of.Record data and treatment group information
It is shown in Table 2 and Fig. 1.
Inhibiting rate of the different biocontrol microorganisms of table 2 to wilt
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. inhibiting rate (%)=(R-r)/R*100%
Method two：Take 5.0 × 107It is treatment 1 that the biocontrol microorganisms 1mL of CFU/ml mixes a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices in the PDA of 99mL, takes 5.0
×107It is treatment 2 that the biocontrol microorganisms 5mL of CFU/ml mixes a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices in the PDA of 95mL.It is with the PDA plates for being not added with any biocontrol microorganisms
Control.The bacterium dish that will be accomplished fluently is as flat board center.25 DEG C of cultures, observe fungi growing state and record fungi growth every three days
Situation.Each treatment is in triplicate.Treatment group information is shown in Table 3 and Fig. 2.
The suppression that the different biocontrol microorganisms of table 3 grow to wilt
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. inhibiting rate (%)=(R-r)/R*100%
Embodiment 2
Bacterial strain JC65, CH01 and SM16 are inoculated into the flat lining outs of LB respectively, 14- is cultivated in 28 DEG C of biochemical cultivation cases
16h, after single bacterium colony is grown, picking single bacterium colony is accessed in the test tube of the LB nutrient solutions containing 5mL, is placed in 28 DEG C, 200r/min
Shaking table in cultivate, be made seed liquor；Seed liquor is inoculated in the triangle of the LB nutrient solutions equipped with 500mL with 1% inoculum concentration
In bottle, 28 DEG C are placed in, 48h is cultivated in the shaking table of 200r/min, 3 kinds of bacterial concentrations are diluted to 5 × 107According to 1 after CFU/mL:
1:1 ratio is well mixed, as described complex microbial inoculum.
The greenhouse Biological control of embodiment 3
The consistent watermelon transplantation of seedlings of growing way is selected when watermelon seedling grows to 3-4 leaf phases in the basin alms bowl containing Nutrition Soil, often
10 watermelon seedlings are transplanted in individual treatment.The same day is transplanted, the 5 × 10 of 20mL have been poured with root-pouring method7The corresponding biocontrol microorganisms of CFU/ml, move
30 days after cultivation biocontrol microorganisms of teeming again.It is control with clear water.Each group growth-promoting situation is observed daily.
The results are shown in Table 4 and Fig. 3.
Total (average) fresh weight=underground part fresh weight+overground part fresh weight
Growth-promoting effect=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] × 100%
Growth-promoting functions (transplant 30d) of the composite bacteria agent LAS of table 4 to watermelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. biomass increase=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] ×
100%
The greenhouse diseases prevention experiment of embodiment 4
When watermelon seedling grows to 3-4 leaf phases, the consistent watermelon transplantation of seedlings of growing way is selected in the basin alms bowl containing Nutrition Soil, often
10 watermelon seedlings are transplanted in individual treatment.The same day is transplanted, 5 × 10 have been poured to the watermelon seedling for the treatment of group with root-pouring method7The phase of CFU/ml
Biocontrol microorganisms are answered, 5 × 10 are poured to the watermelon seedling for the treatment of group with root-pouring method again immediately6The spore suspension 20mL of individual spore/mL.With
Clear water is control.Observation each group diseases prevention situation, is observed with the control group incidence of disease up to stopping after more than 95% daily.Diseases prevention information is shown in
Table 5.
Droop disease level standard：
0 grade：Without illness；
1 grade：Yellowing leaf or wilting area ＜ 50%；
2 grades：Yellowing leaf or wilting area ＞ 50%；
3 grades：Blade is wilted or withered, only growing point survival；
4 grades：Whole pearl is withered.
Disease severity (%)=100% × Σ (sick plant number × disease series)/(total plant number × highest disease series)
Protection effect (%)=100% × (control disease severity-treatment disease severity)/control disease severity
Prophylaxis effects (transplant 30d) of the composite bacteria agent LAS of table 5 to watermelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. prevention effect %=(control disease severity-treatment disease severity)/compare disease severity × 100
The crop field diseases prevention experiment of embodiment 5
Field experiment is carried out in southern agriculture decorated archway Experimental Base.Variety of watermelon is early good (84-24)
Experiment process is：Biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS, clear water control.Processing method：Transplant same day treatment, 15d, 30d, 75d
Processed in the way of pouring root respectively.
Each 3 repetitions for the treatment of, each plot area 24m2(6m*4m), line-spacing is 100cm, and spacing in the rows is 40cm.Each
60 watermelons of cell.Specifying information is shown in Table 6.
Prophylaxis effects (transplant 105d) of the big Tanaka's composite bacteria agent LAS of table 6 to watermelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. prevention effect=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] × 100%
The crop field diseases prevention experiment of cucumber, muskmelon and pumpkin is tested with the diseases prevention of watermelon crop field, and specifying information is shown in Table 6,7,8.
Prophylaxis effects (transplant 105d) of the big Tanaka's composite bacteria agent LAS of table 7 to cucumber
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. prevention effect=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] × 100%
Prophylaxis effects (transplant 105d) of the big Tanaka's composite bacteria agent LA of table 8 to muskmelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. prevention effect=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] × 100%
Prophylaxis effects (transplant 105d) of the big Tanaka's composite bacteria agent LA of table 9 to pumpkin
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. prevention effect=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] × 100%
The crop field growth-promoting effect of embodiment 7
Field experiment is carried out in southern agriculture decorated archway Experimental Base.Variety of watermelon is early good (84-24)
Experiment process is：Biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS, clear water control.Processing method：Transplant same day treatment, 15d, 30d, 75d
Processed in the way of pouring root respectively.
Each 3 repetitions for the treatment of, each plot area 24m2(6m*4m), line-spacing is 100cm, and spacing in the rows is 40cm.Each
60 watermelons of cell.Specifying information is shown in Table 10.
The crop field Biological control of cucumber, muskmelon and pumpkin is shown in Table 11,12,13 with watermelon crop field Biological control, specifying information.
Growth-promoting functions (transplant 35d) of the big Tanaka's composite bacteria agent LAS of table 10 to watermelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. biomass increase=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] ×
100%
Growth-promoting functions (transplant 35d) of the big Tanaka's composite bacteria agent LAS of table 11 to cucumber
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. biomass increase=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] ×
100%
Growth-promoting functions (transplant 35d) of the big Tanaka's composite bacteria agent LAS of table 12 to muskmelon
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. biomass increase=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] ×
100%
Growth-promoting functions (transplant 35d) of the big Tanaka's composite bacteria agent LAS of table 13 to pumpkin
Note：1. numerical value is average value, and between different letter expression treatment under the level of signifiance of P=0.05, otherness is notable.
2. biomass increase=[(treatment mean fresh-clear water control mean fresh)/clear water control mean fresh] ×
100%.
Claims (8)
1. it is a kind of efficiently to prevent and treat the microbial composite of various crop droop, it is characterised in that to be made up of following 3 kinds of bacteriums, point
It is not：Bacillus JC65 (Bacillus sp.), Chinese microorganism strain preservation management is preserved on December 15th, 2010
Committee's common micro-organisms center, culture presevation number is CGMCC No.4475；Bacillus cercus CH01 (Bacillus
Cereuse), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, bacterium on November 17th, 2015
It is CGMCC NO.11677 to plant preserving number；Bacillus subtillis SM16 was (Bacillus subtilis), in 06 month 2009 18
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center day, culture presevation number is CGMCC No.3127.
2. application of the microbial composite described in claim 1 in terms of preventing and treating droop and promoting plant growth.
3. the microbial composite described in claim 1 prevents and treats droop and promotes the microbial composite bacteria of plant growth in preparation
Application in agent.
4. the complex microbial inoculum that prepared by the microbial composite described in claim 1.
5. the complex microbial inoculum described in claim 4, it is characterised in that be prepared by the following method and obtain：By claim
Bacterial strain JC65, CH01 and SM16 described in 1 are inoculated into the flat lining outs of LB respectively, and 14-16h is cultivated in 28 DEG C of biochemical cultivation cases,
After single bacterium colony is grown, picking single bacterium colony is accessed in the test tube of the LB nutrient solutions containing 5mL, is placed in 28 DEG C, and 200r/min's shakes
Cultivated in bed, be made seed liquor；Seed liquor is inoculated in the triangular flask of the LB nutrient solutions equipped with 500mL with 1% inoculum concentration,
28 DEG C are placed in, 48h is cultivated in the shaking table of 200r/min, 3 kinds of bacterial concentrations are diluted to 5 × 107According to 1 after CFU/mL:1:1
Ratio is well mixed, as described complex microbial inoculum.
6. the preparation method of the complex microbial inoculum described in claim 4, it is characterised in that including：By described in claim 1
Bacterial strain JC65, CH01 and SM16 be inoculated into the flat lining outs of LB respectively, cultivate 14-16h in 28 DEG C of biochemical cultivation cases, wait to grow
After single bacterium colony, picking single bacterium colony is accessed in the test tube of the LB nutrient solutions containing 5mL, is placed in 28 DEG C, is trained in the shaking table of 200r/min
Support, be made seed liquor；Seed liquor is inoculated in the triangular flask of the LB nutrient solutions equipped with 500mL with 1% inoculum concentration, is placed in 28
DEG C, 48h is cultivated in the shaking table of 200r/min, 3 kinds of bacterial concentrations are diluted to 5 × 107According to 1 after CFU/mL:1:1 ratio is mixed
Close uniform.
7. application of the complex microbial inoculum described in claim 4 in terms of preventing and treating droop and promoting plant growth.
8. application according to claim 7, it is characterised in that being diluted pouring root when crop is transplanted and sows makes
With.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107586735A (en) * | 2017-08-01 | 2018-01-16 | 环境保护部南京环境科学研究所 | A kind of biocontrol bacterial strain CH01 of efficiently preventing and treating citrus bacterial canker disease and its application |
| CN107646873A (en) * | 2017-10-12 | 2018-02-02 | 泰克美生物科技（喀左）有限公司 | A kind of microbial bacterial agent for preventing and treating bacterial plant disease and application |
| CN108396002A (en) * | 2018-03-07 | 2018-08-14 | 山东新禾丰作物营养有限公司 | One bacillus licheniformis and its application in preventing Muskmelon Fusarium wilt |
| US11555946B2 (en) * | 2019-12-19 | 2023-01-17 | Basf Corporation | Systems and methods for modeling disease severity |
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| CN102174431A (en) * | 2011-01-12 | 2011-09-07 | 南京农业大学 | Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon |
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| CN102524303A (en) * | 2011-08-31 | 2012-07-04 | 南京农业大学 | Mixture for preventing blight and verticillium wilt of cotton |
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| CN107586735A (en) * | 2017-08-01 | 2018-01-16 | 环境保护部南京环境科学研究所 | A kind of biocontrol bacterial strain CH01 of efficiently preventing and treating citrus bacterial canker disease and its application |
| CN107586735B (en) * | 2017-08-01 | 2020-04-28 | 环境保护部南京环境科学研究所 | Biocontrol strain CH01 for efficiently preventing and treating citrus canker and application thereof |
| CN107646873A (en) * | 2017-10-12 | 2018-02-02 | 泰克美生物科技（喀左）有限公司 | A kind of microbial bacterial agent for preventing and treating bacterial plant disease and application |
| CN108396002A (en) * | 2018-03-07 | 2018-08-14 | 山东新禾丰作物营养有限公司 | One bacillus licheniformis and its application in preventing Muskmelon Fusarium wilt |
| CN108396002B (en) * | 2018-03-07 | 2019-12-06 | 山东新禾丰作物营养有限公司 | Bacillus licheniformis and application thereof in preventing and treating sweet melon fusarium wilt |
| US11555946B2 (en) * | 2019-12-19 | 2023-01-17 | Basf Corporation | Systems and methods for modeling disease severity |
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