CN106754544A - One plant of streptomyces lydicus for preventing and treating droop and its application - Google Patents
One plant of streptomyces lydicus for preventing and treating droop and its application Download PDFInfo
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- CN106754544A CN106754544A CN201710022672.6A CN201710022672A CN106754544A CN 106754544 A CN106754544 A CN 106754544A CN 201710022672 A CN201710022672 A CN 201710022672A CN 106754544 A CN106754544 A CN 106754544A
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- streptomyces lydicus
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- fusarium wilt
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The present invention relates to microbial manure technical field, more particularly, to one plant of streptomyces lydicus for preventing and treating droop and its application, the strain classification is named as streptomyces lydicus (Streptomyces lydicus), in the preservation of October 12 in 2016 to Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation number：CGMCC No.13106.Streptomyces lydicus of the invention has obvious antagonistic effect to Muskmelon Fusarium wilt, is one plant of biocontrol bacterial strain having a extensive future, can be used as the function stem for preparing microbial manure.
Description
Technical field
The present invention relates to microbial manure technical field, more particularly, to one plant of streptomyces lydicus for preventing and treating droop and its
Using.
Background technology
Muskmelon is one of big fruit in the world ten, and its fruit is delicious fragrant and sweet, deep to be liked by the people of various countries.With Muskmelon Planting
Area, planting density are continuously increased, and muskmelon is faced with while progressively increasing production, also the danger that pest and disease damage is constantly aggravated, its
Middle Muskmelon Fusarium wilt is one of most serious disease of influence muskmelon production.Muskmelon Fusarium wilt is a kind of soil-borne disease, for the first time quilt
The U.S. SouthCarolina in 1894 is the discovery that, different degrees of wilt disease is all occurred in that all over the world afterwards.1949
Year, U.S. Florida is because the muskmelon underproduction is more than 40% (Booth, C.Fusarium caused by droop:laboratory
guide to the identification of the major species[J].Commonwealth Mycological
Institute,1977).(Zhang Shumei, Zhao Xiaoyu, Zhang Xiancheng wait .3 kind Fusarium oxysporums Fusarium to Zhang Shumei etc.
Molecular Detection [J] Plant Pathologies of oxysporum, 2010,40 (6):636-641) article delivered for 2010 is just carried
Go out, Melon fusarium Wilt F.oxysporum in the form of spore due to existing, and vitality is more long, once existing sweet in the earth of native land
Cucurbit wilt bacterium F.oxysporum is general to be difficult to be uprooted.
At present, the preventing and treating of Muskmelon Fusarium wilt is mainly chemical agent, although chemical prevention can quickly eliminate pathogen, easily
Muskmelon Plants drug resistance is caused, melon fruit residues of pesticides are exceeded, environmental pollution, and then have influence on the safety and Health of people.Cause
This, seeks green, the droop prevention and controls of environmental protection, is a vital task during Development of Muskmelon Industry.Biological control
It is the safe prevention method of a kind of " fungus treatment ", it is nontoxic to environment and people, and economic input is relatively low, is now subjected to
Increasing people's concern.
My laboratory filters out one plant to Muskmelon Fusarium wilt with good anti-from the Muskmelon Planting soil of the cities and counties of Hainan 11
The streptomyces lydicus of effect, the fermented liquid is to Muskmelon Fusarium wilt prevention effect up to 71.56%.Muskmelon Fusarium wilt evil generation with
Weather, soil property, agricultural irrigation measure etc. have a great impact, and the streptomyces lydicus of my laboratory screening to a certain extent can be with rich
The biocontrol microorganisms species of rich droop, for the biological control of torrid areas Muskmelon Fusarium wilt provides certain guidance foundation.
The content of the invention
For the deficiencies in the prior art, it is an object of the invention to provide one plant of sharp enlightening chain of preventing and treating Muskmelon Fusarium wilt
Mould, and its application in preventing and treating Muskmelon Fusarium wilt evil.
To achieve the above object, the technical solution adopted by the present invention is：One plant of streptomyces lydicus for preventing and treating droop, the bacterium
Strain Classification And Nomenclature is streptomyces lydicus (Streptomyces lydicus), is exposed to the sun in the preservation of October 12 in 2016 to Beijing
The institute 3 of area North Star West Road 1 China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation number：
CGMCC No.13106。
Streptomyces lydicus microbial inoculum and streptomyces lydicus bacterium powder that those skilled in the art are prepared by conventional biological method
Also it is within the scope of the present invention.
The present invention also provides a kind of application of streptomyces lydicus in preventing and treating Muskmelon Fusarium wilt evil.
The streptomyces lydicus is in mid-September, 2011 from 11, Hainan Province cities and counties (Sanya, Lingshui, Bao Ting, Le Dong, the five fingers
Mountain, Danzhou, white sand, Lingao, Chengmai, Wenchang, Qionghai) screen separating obtained in melonry soil, its main biological property：Bacterium
Plant shape is into aerial hyphae and fibrillae of spores, fibrillae of spores spirality, steamed stuffed bun oval, ellipse.Major physiological biochemical characteristic：In yeast
Well-grown on the culture mediums such as smart malt extract agar, sucrose nitrate agar.
Using BLAST (http:/ www.Ncbi.nlm.nih.gov) by streptomyces lydicus bacterial strain 16S rRNA gene orders
Compare with sequence in GenBank, and select representational type sepecies phylogenetic tree construction.Result shows, the bacterial strain with
The homology of Streptomyces lydicus NBRC 13058T (AB184281) determines that the bacterial strain is sharp enlightening chain up to 99.6%
Mould.
The method have the benefit that：Streptomyces lydicus of the invention has obvious antagonism to imitate to Muskmelon Fusarium wilt
Really, it is one plant of biocontrol bacterial strain having a extensive future, can be used as the function stem for preparing microbial manure.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
Other accompanying drawings are obtained with according to these accompanying drawings.
Fig. 1 is the colonial morphology figure of streptomyces lydicus；
Fig. 2 is streptomyces lydicus antagonizing pathogenic fungi experimental result picture；A upper left side：Streptomyces lydicus is to muskmelon pathogen
FJAT-9230；It is upper right：Muskmelon pathogen FJAT-9230；Bottom left：Streptomyces lydicus is to muskmelon pathogen FJAT-9062；Bottom right：
Muskmelon pathogen FJAT-9062；
Fig. 3 is streptomyces lydicus genetic stability experimental result picture.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Embodiment 1：The activation culture of streptomyces lydicus
After using inserted sheet method culture streptomyces lydicus 5,7,10d, observe its aerial hyphae, fibrillae of spores and spore filament shapes,
Spore shapes (Fig. 1)：Bacterial strain forms aerial hyphae and fibrillae of spores, fibrillae of spores spirality, steamed stuffed bun oval, ellipse.
Streptomyces lydicus is seeded in Gause I agar, glycerine asparagus fern element agar, inorganic salts starch agar, yeast malt
On the culture mediums such as smart agar, oatmeal agar, sucrose nitrate agar, glucose-asparagine agar, tyrosine agar, in
After 28 DEG C of culture 7d, start to observe the cultural characteristic on various culture mediums, be specifically shown in Table 1.Its physio-biochemical characteristics is detected, specifically
It is shown in Table 2.
Feature of the streptomyces lydicus of table 1 on different culture media
The physiological and biochemical property of the streptomyces lydicus bacterial strain of table 2
Note："+" table is positive, and "-" table is negative.
Embodiment 2：Methylotrophic bacillus antagonizing pathogenic fungi is tested
Flat board face-off experiment：With Melon fusarium Wilt FJAT-9062, FJAT-9230 as indicator bacteria, -4 DEG C of rings will be stored in
Bacterial strain under border, is beaten with card punch and takes 5mm bacteria cakes point and be connected in PDA culture medium (potato 200g, glucose 20g, agar
15g, deionized water 1000ml) culture 3d pathogen flat board surrounding, point meet place apart from pathogen center 2cm, each flat board point 4
Place, its antagonism index is determined after culture 4d.Statistics shows that streptomyces lydicus is to Muskmelon Fusarium wilt FJAT-9062, FJAT-
9230 antagonism index is respectively 3.46,2.41, and its antagonistic effect is shown in Fig. 2.
, as Antagonistic Fungi to a kind of representation of pathogen antagonistic effect, antagonism index is bigger, Antagonistic Fungi for antagonism index
Antagonistic effect to pathogen is better, conversely, then antagonistic effect is poorer.Antagonism of this experiment streptomyces lydicus to Muskmelon Fusarium wilt
Index is all higher than 2, and antagonistic effect is fine, and streptomyces lydicus is higher than FJAT- to the antagonistic effect of Muskmelon Fusarium wilt FJAT-9062
9230。
Embodiment 3：Methylotrophic bacillus genetic stability is tested
Squamous subculture experiment is carried out to streptomyces lydicus according to flat board face-off experimental technique, its antagonism index is observed by generation
Change, so that it is determined that the genetic stability of strain.This experiment is to the streptomyces lydicus first generation, the 5th generation, the tenth generation, the 15th
Generation, the 20th generation antagonism index are determined, and as a result see Fig. 3.From the figure 3, it may be seen that Methylotrophic bacillus is the 20th
Dai Shi, still has antagonistic effect to Muskmelon Fusarium wilt FJAT-9062, FJAT-9230, and streptomyces lydicus is illustrated to a certain extent
Antagonistic effect to Muskmelon Fusarium wilt FJAT-9062, FJAT-9230 is more lasting, is conducive to the bacterium in a long time to this
Pathogen plays inhibitory action.
Embodiment 4：The influence that Methylotrophic bacillus sprouts to seed
Inoculation under -4 DEG C of environment to PDB culture mediums (potato 200g, glucose 20g, deionized water will be stored in
In 1000mL), shaking table shaken cultivation 24h obtains final product Antagonistic Fungi bacteria suspension；The full South Sea honey muskmelon seedses of picking, use 10%H2O2
Surface sterilization 5min is carried out to seed, afterwards with sterile water wash 3-4 times, then seed 2h in Antagonistic Fungi bacteria suspension is soaked in, together
Shi Yiwei adds the PDB culture mediums of Antagonistic Fungi to compare, and often processes 30 muskmelon seedses, three repetitions；After immersion is finished, will be sweet
On the moistening aseptic filter paper that melon seed is placed in sterilizing culture dish, one layer of wet gauze is added a cover on seed, 30 DEG C of vernalization 36h are calculated
The germination percentage of seed, statistics is shown in Table 3.
The influence that the streptomyces lydicus of table 3 is sprouted to seed
Test result indicate that, streptomyces lydicus does not influence the germination of muskmelon seedses, and can improve muskmelon to a certain extent
Percentage of seedgermination, illustrates that streptomyces lydicus is nontoxic to muskmelon seedses, and its bacteria suspension has certain promotion to make to muskmelon seedses
With.
Embodiment 5：Streptomyces lydicus antagonism Muskmelon Fusarium wilt potted plant experiment
Choose 3 consistent leaf Muskmelon Seedlings of growing way to be transplanted in basin alms bowl, after transplanting 7 days, muskmelon seedling carried out to connect bacterium treatment,
Each 3 repetition for the treatment of, each repeats 10 basins, per 1 plant of muskmelon seedling of basin.Connect bacterium 7 days afterwards, record muskmelon incidence, calculate
Its disease index and relative prevention effect, statistics are shown in Table 3, table 4.Each treatment is as follows：
Treatment 1：First it is inoculated with 107Cfu/mL Pathogen of Fusarium Wilt suspension FJAT-906210mL, inoculated every 1 day
107Cfu/mL streptomyces lydicus suspensions 10mL；
Treatment 2：First it is inoculated with 107Cfu/mL streptomyces lydicus suspension 10mL, 10 were inoculated every 1 day7Cfu/mL droops disease
Opportunistic pathogen suspension FJAT-906210mL；
Control treatment (CK)：Pathogen of Fusarium Wilt suspension is only inoculated with, labeled as CK.
Muskmelon Fusarium wilt incidence is divided into 5 grades by standard：0 grade：Complete stool is disease-free；1 grade：Plant blade face table below 1/4
Now wilt symptom；2 grades：The blade face of plant 1/4 to 1/2 shows wilting symptom；3 grades：The blade face of plant more than 1/2 shows wilting symptom：4
Level：Complete stool is wilted dead.
Disease index=[Σ (the sick level strain number × disease series)/(representative of the treatment Potted orchard summation × morbidity most heavy duty
Numerical value)] × 100.
With respect to prevention effect=[(control disease index-treatment disease index)/control disease index] × 100%.
The Muskmelon Fusarium wilt incidence of table 4 is counted
Prevention effect of the streptomyces lydicus of table 5 to Muskmelon Fusarium wilt
Result above shows that first the muskmelon disease index of access pathogen treatment is less than and first connects disease after access streptomyces lydicus
The treatment of bacterial strain is accessed after opportunistic pathogen, illustrates that streptomyces lydicus has certain prevention to Muskmelon Fusarium wilt to a certain extent
Effect.In addition, accessing before either first pathogen or afterwards streptomyces lydicus, its prevention effect is above control, wherein
Its prevention effect of the treatment of streptomyces lydicus is first connect up to 71.56%, the treatment of streptomyces lydicus its prevention effect is accessed afterwards is
63.68%.It can be seen that, streptomyces lydicus of the invention has certain prevention and prevention effect to Muskmelon Fusarium wilt.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, all terms used herein (including technology art
Language and scientific terminology) have with art of the present invention in those of ordinary skill general understanding identical meaning.Should also
Understand, those terms defined in such as general dictionary should be understood that the meaning having with the context of prior art
The consistent meaning of justice, and unless defined as here, will not be with idealizing or excessively formal implication be explained.
It should be noted last that：Above example is only used to illustrative and not limiting technical scheme, although ginseng
The present invention has been described in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that：Still can be to this
Invention is modified or equivalent, any modification or partial replacement without departing from the spirit and scope of the present invention, and its is equal
Should cover in the middle of scope of the presently claimed invention.
Claims (4)
1. one plant of streptomyces lydicus for preventing and treating droop, it is characterised in that the strain classification is named as streptomyces lydicus
(Streptomyces lydicus), in the preservation of October 12 in 2016 to Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China
Microbiological Culture Collection administration committee common micro-organisms center (CGMCC), preservation number：CGMCC No.13106.
2. it is a kind of to be prepared into microbial inoculum using streptomyces lydicus described in claim 1.
3. it is a kind of to be prepared into bacterium powder using streptomyces lydicus described in claim 1.
4. application of the streptomyces lydicus in preventing and treating Muskmelon Fusarium wilt evil according to claim any one of 1-3.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109837228A (en) * | 2019-04-03 | 2019-06-04 | 南京工业大学 | One plant of streptomyces lydicus and its application in prevention and treatment fire blast |
| CN112746038A (en) * | 2020-12-16 | 2021-05-04 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W02 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611283A (en) * | 2012-12-27 | 2015-05-13 | 北京市农林科学院 | Recombinational streptomyces lydicus and application thereof |
-
2017
- 2017-01-12 CN CN201710022672.6A patent/CN106754544A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611283A (en) * | 2012-12-27 | 2015-05-13 | 北京市农林科学院 | Recombinational streptomyces lydicus and application thereof |
Non-Patent Citations (2)
| Title |
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| 卢彩鸽 等: "拮抗放线菌A02活性产物的分离和鉴别特性研究", 《华北农学报》 * |
| 邹立飞: "甜瓜枯萎病病原拮抗菌的筛选及利用响应面法优化发酵条件", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109837228A (en) * | 2019-04-03 | 2019-06-04 | 南京工业大学 | One plant of streptomyces lydicus and its application in prevention and treatment fire blast |
| CN109837228B (en) * | 2019-04-03 | 2020-03-03 | 南京工业大学 | Streptomyces lydicus and application thereof in prevention and treatment of fire blight |
| CN112746038A (en) * | 2020-12-16 | 2021-05-04 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W02 and application thereof |
| CN112746038B (en) * | 2020-12-16 | 2022-11-29 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W02 and application thereof |
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