CN106754369A - Blake bottle - Google Patents
Blake bottle Download PDFInfo
- Publication number
- CN106754369A CN106754369A CN201611195455.9A CN201611195455A CN106754369A CN 106754369 A CN106754369 A CN 106754369A CN 201611195455 A CN201611195455 A CN 201611195455A CN 106754369 A CN106754369 A CN 106754369A
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- CN
- China
- Prior art keywords
- sleeve
- support tube
- blake bottle
- action bars
- annulus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Mixers Of The Rotary Stirring Type (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of blake bottle, including body and bottom, bottom are removably fastened on body lower end, and body bottom is provided with annulus, and bottom includes bottom wall and side wall, and side wall is provided with the tightening device overlapped with annulus, and sidewall upper is provided with the first sealing gasket with annulus contact site；Inside bottom wall face is installed by the first support tube, first support tube upper end is installed by the first sleeve, first sleeve outside is provided for washing away the scouring mechanism of attached cell, first sleeve upper end is installed by second sleeve, second sleeve lower outside is provided for the current stabilization stirring mechanism of nutrient solution mixing, second sleeve upper end is installed by the second support tube, rotor plate is installed after passing through bottle cap in the second support tube upper end, rotor plate can drive second sleeve and current stabilization stirring mechanism to rotate by the second support tube, and the action bars for driving the first sleeve and scouring mechanism rotation is installed in second sleeve.Current stabilization stirring mechanism and scouring mechanism can be operated individually in the present invention, it is also possible to which be connected action simultaneously.
Description
Technical field
The present invention relates to a kind of labware, more particularly, to a kind of blake bottle of cell and tissue in vitro culture.
Background technology
In teaching and research, the in vitro culture of cell and tissue is often carried out, the cell type of culture is various, from disease
Poison to bacterium and fungi, from human body cell to zooblast and plant cell.It is different with research purpose according to cell characteristics, can use
In provide cell culture container including blake bottle, culture plate and culture dish etc..Some cells and organization need are in suspended state
Lower growth, but most of mammalian cell needs surface adherent, therefore from applicable performance, can simply by blake bottle point
It is suspension blake bottle and adhere-wall culture bottle.
The larger blake bottle of existing volume, in order to the growth for ensureing cell and tissue is typically provided with stir plate in bottle,
Stir plate is connected by swingle with bottle cap, stir plate mixed-culture medium is driven by bottle cap, to keep the equal of the growths such as cell
Weighing apparatus property.But existing stir plate structure is more single, limited to the effect of nutrient solution mixing, it is difficult to meet making for different blake bottles
With requiring；In addition, the size of stir plate and bottleneck size will be adapted to, also therefore, it will usually do smaller of stir plate or
The bottleneck of blake bottle is opened greatly, stir plate is smaller, mixed effect can be reduced；And that bottleneck is opened up is larger, be not easy to it is actual plus
Liquid, take liquid etc. operation.
The content of the invention
The present invention provides a kind of blake bottle, single for solving stir plate structure in the prior art, nutrient solution is mixed and is imitated
Really poor problem.
The present invention is achieved by the following technical programs：
Blake bottle, including body and bottom, bottom are removably fastened on body lower end, and the body top is necking down structure
Shoulder, shoulder upper end forms bottleneck, and bottle cap is installed on bottleneck, and the body lower outside is provided with annulus, and the bottom includes bottom
Wall and side wall, on the wall of side at least provided with a pair with the tightening device of annulus overlap joint, sidewall upper and annulus contact site are provided with the
One sealing gasket；The first vertical support tube is installed, the first sleeve is installed in the first support tube upper end in the middle of the inside bottom wall face, the
One sleeve outside is provided for washing away the scouring mechanism of attached cell, and the first sleeve and the first support tube are connected by clutch shaft bearing
Connect, clutch shaft bearing inner ring is connected with the first sleeve lower end, its outer ring is fixed with the first support tube upper end；The first sleeve upper end
Second sleeve is installed, second sleeve lower outside is provided for the current stabilization stirring mechanism of nutrient solution mixing, the first sleeve and second
Sleeve is connected by second bearing, and second bearing inner ring is connected with second sleeve lower end, and its outer ring is fixed with the first sleeve upper end,
The second support tube is installed in the second sleeve upper end, the second support tube upper end through installing rotor plate after bottle cap, its lower end and the
Two sleeve upper ends are connected by fixture block, notch, and rotor plate can drive second sleeve and current stabilization to stir by the second support tube
Motivation structure rotates, and the action bars for driving the first sleeve and scouring mechanism rotation is installed in the second sleeve, on action bars
End is higher by rotation plate top surface.
In order to realize the purpose of the present invention, can also adopt the following technical scheme that：
Blake bottle as described above, the current stabilization stirring mechanism includes short sleeve and outer ring, symmetrically pacifies between outer ring and short sleeve
Multiple inclined swirl vanes are filled, the upper edge of swirl vane is located at the tangential direction of short sleeve horizontal cross-section, and the short sleeve is consolidated
It is scheduled on second sleeve periphery.
Blake bottle as described above, the scouring mechanism includes helical blade, and helical blade is provided with drawing for multiple transverse directions
Chute, the bottom land of drainage trough outwards arches upward in the flexure plane of helical blade.
Blake bottle as described above, the vertical range of the helical blade outermost point to the first sleeve axis is body diameter
1/2 ~ 4/5 times；The drainage trough is provided with more than 3 roads.
Blake bottle as described above, the first support tube inside upper part is provided with spacing ring, and the downside of spacing ring is installed and lived
Dynamic sealing plate, sets second spring, the lower end of the action bars and movable sealing in the first support tube below movable sealing plate
Plate is contacted.
Blake bottle as described above, the rotor plate is provided with symmetrical link slot, action bars in the outside left of action bars
It is provided with the contiguous block coordinated with link slot；The action bars top is installed by bar blocks.
Blake bottle as described above, the position that the action bars is located in the first sleeve is provided with the deep hole of radial direction, in deep hole
The first spring and ball are provided with, the first both ends of the spring is connected with deep hole bottom, ball respectively, the semi-circular section of ball is more than deep hole
Outermost openings of sizes, first sleeve lining is provided with locating slot, and locating slot and ball engagement are by action bars and first set
Cylinder links into an integrated entity action.
Blake bottle as described above, the width of the annulus is 1 ~ 1.5cm, and the body lower end contacts and be provided with bottom wall
Second sealing gasket.
Blake bottle as described above, the bottleneck inner side is provided with the sealing-plug with bottle cap one, be provided with the middle of sealing-plug with
The passage that second support tube coordinates.
Blake bottle as described above, the inside bottom wall face is provided with installs fixed block in groove, groove, be provided with fixed block
Axis hole, under first support tube end closure and be provided with insertion axis hole rotating shaft.
It is an advantage of the invention that：
1st, body and bottom are arranged to Split type structure by the present invention, and bottom can closely be fastened on body lower end, therefore can set
Larger nutrient solution stirring mechanism, without being limited by the limitation of bottleneck size, is easy to stirring mechanism installation, body and bottom to clean
Operated Deng using.
2nd, because bottom is fastened on body lower end, outside raised structures are formed relative to body, increases available connecing
Contacting surface is accumulated, therefore can improve overall stability, prevents from toppling over damage.
3rd, current stabilization stirring mechanism and scouring mechanism are provided with the inside of body, current stabilization stirring mechanism can be formed in zone line
Eddy flow, makes bottom culture, middle part nutrient solution and top nutrient solution form complete mixing and is produced without the cell to adhere-wall culture
Influence；Scouring mechanism can form preferable peeling effect to attached cell, remove scraper plate operation from, be easy to sample and use.Surely
Stream stirring mechanism and scouring mechanism can be operated individually, it is also possible to which be connected action simultaneously, thus can be according to difference
Purpose and requirement are adjusted.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described.
Fig. 1 is structural representation of the invention.
Fig. 2 is the A-A of Fig. 1 to amplification view.
Fig. 3 is the B-B direction amplification view of Fig. 1（The helical blade not shown in figure）.
Fig. 4 is the C of Fig. 1 to schematic diagram（The body and bottom not shown in figure）
Fig. 5 is the internal structure schematic diagram of the first support tube and the first sleeve connecting portion described in Fig. 1.
Reference：1- bodies；11- annulus；2- bottoms；21- bottom walls, 22- sides wall, 23- tightening devices, 24- first is close
Packing, the sealing gaskets of 25- second, the support tubes of 3- first, 31- clutch shaft bearings, 32- spacing rings, 33- movable sealing plates, the bullets of 34- second
Spring, the sleeves of 4- first, 41- helical blades, 42- drainage troughs, 43- locating slots, 5- second sleeves, 51- short sleeves, 52- outer rings,
53- swirl vanes, the support tubes of 6- second, 7- rotor plates, 71- link slots, 8- action bars, 81- deep holes, the springs of 82- first, 83-
Ball, 84- contiguous blocks, 85- bar blocks, 9- fixed blocks, 10- bottle caps.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
A part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
As Figure 1-Figure 5, a kind of blake bottle of the present embodiment, including body 1 and bottom 2, bottom 2 are removably fastened on
The lower end of body 1, the top of body 1 is the shoulder of necking down structure, and shoulder upper end forms bottleneck, bottle cap 10 is installed on bottleneck, under body 1
Portion outside is provided with annulus 11, and bottom 2 includes bottom wall 21 and side wall 22, at least provided with a pair and the button of the overlap joint of annulus 11 on side wall 22
Tight device 23, the upper end of side wall 22 is provided with the first sealing gasket 24 with the contact site of annulus 11；Installed in the middle of the medial surface of bottom wall 21 vertical
The first support tube 3, the upper end of the first support tube 3 installs the first sleeve 4, and the outside of the first sleeve 4 is provided for washing away attached cell
Scouring mechanism, the first sleeve 4 is connected with the first support tube 3 by clutch shaft bearing 31, the inner ring of clutch shaft bearing 31 and the first sleeve 4
Lower end connects, and its outer ring 52 is fixed with the upper end of the first support tube 3；Second sleeve 5 is installed in the upper end of first sleeve 4, under second sleeve 5
Portion outside is provided for the current stabilization stirring mechanism of nutrient solution mixing, and the first sleeve 4 and second sleeve 5 are connected by second bearing,
Second bearing inner ring is connected with the lower end of second sleeve 5, and its outer ring 52 is fixed with the upper end of the first sleeve 4, and the upper end of second sleeve 5 is installed
Second support tube 6, through installation rotor plate 7 after bottle cap 10, its lower end passes through the upper end of the second support tube 6 with the upper end of second sleeve 5
Fixture block, notch connection, rotor plate 7 can drive second sleeve 5 and current stabilization stirring mechanism to rotate by the second support tube 6,
Action bars 8 for driving the first sleeve 4 and scouring mechanism rotation is installed, the upper end of action bars 8 is higher by rotor plate in second sleeve 5
7 top surfaces.
Specifically, blake bottle disclosed in the present embodiment, Split type structure is arranged to by body 1 and bottom 2, and bottom 2 can be tight
It is close to be fastened on the lower end of body 1, therefore larger nutrient solution stirring mechanism can be set, without being limited by the limitation of bottleneck size,
It is easy to stirring mechanism installation, body 1 and the cleaning of bottom 2 etc. to use operation.As shown in figure 1, the width of annulus 11 is 1cm, body 1
Lower end contacts with bottom wall 21 and is provided with the second sealing gasket 25, because bottom 2 is fastened on the lower end of body 1, relative to body 1 formed to
Outer raised structures, increase available contact area, therefore can improve overall stability, prevent from toppling over damage.In bottle
The inner side of body 1 is provided with current stabilization stirring mechanism and scouring mechanism, and current stabilization stirring mechanism can form eddy flow, train bottom in zone line
Support, middle part nutrient solution and top nutrient solution are formed and completely mixed without the affecting cells to adhere-wall culture；Scouring mechanism
Preferable peeling effect can be formed to attached cell, removes scraper plate operation from, be easy to sample and use.Current stabilization stirring mechanism and punching
Brush mechanism can individually operate, it is also possible to which be connected action simultaneously, thus can be carried out according to different purposes and requirement
Regulation.Wherein, as shown in figure 5, the connection of the first sleeve and second sleeve, with the first support tube 3 and the connection knot of the first sleeve
Structure is similar to, and the lower end of clutch shaft bearing 31, second bearing inner ring respectively with the first sleeve, second sleeve can use chute and sliding block
Structure, is easy to grafting and installation, is supported in order to avoid nutrient solution enters the first sleeve, second sleeve, the first support tube 3, second
In pipe 6, sealing device should be set at the interconnection position of above-mentioned part.
When using, the first support tube 3 is fixedly connected with bottom wall 21 first, then successively in the top of the first support tube 3 peace
The first sleeve and scouring mechanism, second sleeve and current stabilization stirring mechanism are filled, bottom 2 is then fastened on the lower end of body 1, by button
Tight device 23 is realized fixing, and then by bottleneck to adding nutrient solution in body 1, then makes in the second support tube 6 and second sleeve 5
End connection, covers bottle cap 10 and can be used.During cell culture, rotor plate or action bars is operated to drive as needed steady
Stream stirring mechanism or scouring mechanism action.
As shown in figure 3, the current stabilization stirring mechanism in the present embodiment includes short sleeve 51 and outer ring 52, outer ring 52 and short sleeve
Multiple inclined swirl vanes 53 are symmetrically installed between 51, the upper edge of swirl vane 53 is located at the tangent line of the horizontal cross-section of short sleeve 51
Direction, short sleeve 51 is fixed on the periphery of second sleeve 5.Nutrient solution from the upper and lower between adjacent swirl vane 53 to flowing simultaneously
Eddy flow effect is formed, relative to the inwall of body 1 due to progressive, its declines of eddy flow, is conducive to the growth of attached cell, and
Can be good at realizing the mixing regulation of nutrient solution simultaneously.Current stabilization stirring mechanism may be used also in addition to using the structure of swirl vane 53
Constituted with the eddy flow line using spring-like.
As shown in figure 1, the scouring mechanism in the present embodiment includes helical blade 41, helical blade 41 is provided with multiple horizontal
Drainage trough 42, the bottom land of drainage trough 42 outwards arches upward in the flexure plane of helical blade 41.Helical blade 41 is stirred relative to current stabilization
Dynamic component, its width is larger, simultaneously because the effect of drainage trough 42, helical blade 41 can produce horizontal cutting stream in action
Effect, thus preferable scouring effect can be formed to the inwall of body 1.
Further, the outermost point of helical blade 41 to the vertical range of the axis of the first sleeve 4 is that body 1 is straight in the present embodiment
3/4 times of footpath；Drainage trough 42 is provided with 3 roads.By the calculating and sunykatuib analysis of Fluid Mechanics, now by helical blade 41
Optimal scouring effect can be formed to the inwall of body 1.
As shown in figure 4, the present embodiment is provided with symmetrical link slot 71 in rotor plate 7 in the outside left of action bars 8, operation
Bar 8 is provided with the contiguous block 84 coordinated with link slot 71；The top of action bars 8 is installed by bar blocks 85.Contiguous block on action bars 8
84 with rotor plate 7 on link slot 71 coordinate when, rotor plate 7 and action bars 8 are formed and are connected integrally, while rotary motion plate 7,
The also concomitant rotation of action bars 8, now, current stabilization stirring mechanism and scouring mechanism are acted together；And work as the contiguous block 84 on action bars 8
When being separated with link slot 71, because scouring mechanism and action bars 8 form one, current stabilization stirring mechanism by the second support tube 6 with
Rotor plate 7 is connected, and scouring mechanism is located at the lower section of current stabilization stirring mechanism, therefore scouring mechanism and current stabilization stirring mechanism can be divided
It is not individually operated, it is easy to use.
As shown in figure 4, the present embodiment is provided with the deep hole 81 of radial direction, deep hole at the position that action bars 8 is located in the first sleeve 4
The first spring 82 and ball 83 are provided with 81, the two ends of the first spring 82 are connected with the bottom of deep hole 81, ball 83 respectively, ball 83
Semi-circular section is more than the outermost openings of sizes of deep hole 81, and the inwall of the first sleeve 4 is provided with locating slot 43, locating slot 43 and ball 83
Coordinate and the sleeve 4 of action bars 8 and first links into an integrated entity action.Action bars 8 can be used with the attachment structure of the first sleeve 41
Ball 83 and locating slot 43 coordinate, it would however also be possible to employ other to be realized as sliding block and chute coordinate, and are connected using ball 83, are easy to
Action bars 8 is installed, and sets locating slot 43, can improve 43 pairs of extruding forces of the first sleeve 4 of ball, it is ensured that action bars 8 and first set
The stability of the connection of cylinder 4.
Further, as shown in figure 5, the inside upper part of the first support tube of the present embodiment 3 is provided with spacing ring 32, spacing ring 32
Downside installation activity sealing plate 33, second spring 34 is set in the first support tube 3 of the lower section of movable sealing plate 33, action bars 8
Lower end contacts with movable sealing plate 33.Moved down in action bars 8 and realize that contiguous block 84 is matched somebody with somebody with link slot 71, ball 83 and locating slot 43
Close, rotor plate 7 and action bars 8 are formed after being connected entirety, and action bars 8 can continue downwards to press when needing to separate with rotor plate 7
Press operation bar, second spring 34 in the first support tube 3 upwardly movable sealing plate 33, movable sealing plate 33 promotes action bars
8 is upward, it is ensured that ball 83 more smoothly departs from from locating slot 43；Movable sealing plate 33 is set and second spring 34 also can customer service
There is misoperation in the influence of the Action of Gravity Field of action bars 8, be easy to use and operate.
As shown in figure 1, the present embodiment is provided with the sealing-plug with the one of bottle cap 10 on the inside of bottleneck, be provided with the middle of sealing-plug with
The passage that second support tube 6 coordinates, provides relatively stable support can to the second support tube 6 by sealing-plug.Further, it is
Preferable fixation is improved to the first support tube 3, the present embodiment is provided with bottom wall 21 medial surface and fixed block is installed in groove, groove
9, axis hole is provided with fixed block 9, the first end closure of support tube 3 times is simultaneously provided with the rotating shaft for inserting axis hole.
The technology contents of the not detailed description of the present invention are known technology.
Claims (10)
1. blake bottle, including body and bottom, bottom are removably fastened on body lower end, and the body top is necking down structure
Shoulder, shoulder upper end formed bottleneck, bottle cap is installed, it is characterised in that the body lower outside is provided with annulus, institute on bottleneck
Stating bottom includes bottom wall and side wall, and at least provided with a pair and the tightening device of annulus overlap joint on the wall of side, sidewall upper connects with annulus
Contact portion position is provided with the first sealing gasket；The first vertical support tube, the first support tube upper end peace are installed in the middle of the inside bottom wall face
The first sleeve is filled, the first sleeve outside is provided for washing away the scouring mechanism of attached cell, and the first sleeve and the first support tube are logical
Clutch shaft bearing connection is crossed, clutch shaft bearing inner ring is connected with the first sleeve lower end, and its outer ring is fixed with the first support tube upper end；It is described
Second sleeve is installed in first sleeve upper end, and second sleeve lower outside is provided for the current stabilization stirring mechanism of nutrient solution mixing, the
One sleeve and second sleeve are connected by second bearing, and second bearing inner ring is connected with second sleeve lower end, its outer ring and first
Sleeve upper end is fixed, and the second support tube is installed in the second sleeve upper end, and the second support tube upper end rotates through installation after bottle cap
Plate, its lower end is connected with second sleeve upper end by fixture block, notch, and rotor plate can drive second by the second support tube
Sleeve and current stabilization stirring mechanism are rotated, and the operation for driving the first sleeve and scouring mechanism rotation is installed in the second sleeve
Bar, action bars upper end is higher by rotation plate top surface.
2. blake bottle according to claim 1, it is characterised in that the current stabilization stirring mechanism includes short sleeve and outer ring,
Multiple inclined swirl vanes are symmetrically installed between outer ring and short sleeve, the upper edge of swirl vane is located at short sleeve horizontal cross-section
Tangential direction, the short sleeve is fixed on second sleeve periphery.
3. blake bottle according to claim 1 and 2, it is characterised in that the scouring mechanism includes helical blade, helical-blade
Piece is provided with the drainage trough of multiple transverse directions, and the bottom land of drainage trough outwards arches upward in the flexure plane of helical blade.
4. blake bottle according to claim 3, it is characterised in that the helical blade outermost point to the first sleeve axis
Vertical range is 1/2 ~ 4/5 times of body diameter；The drainage trough is provided with more than 3 roads.
5. blake bottle according to claim 4, it is characterised in that the first support tube inside upper part is provided with spacing ring,
The downside installation activity sealing plate of spacing ring, sets second spring, the operation in the first support tube below movable sealing plate
The lower end of bar contacts with movable sealing plate.
6. blake bottle according to claim 1, it is characterised in that it is right that the rotor plate is provided with the outside left of action bars
The link slot of title, action bars is provided with the contiguous block coordinated with link slot；The action bars top is installed by bar blocks.
7. blake bottle according to claim 1, it is characterised in that the position that the action bars is located in the first sleeve is provided with
The deep hole of radial direction, is provided with the first spring and ball in deep hole, the first both ends of the spring is connected with deep hole bottom, ball respectively, ball
Semi-circular section be more than the outermost openings of sizes of deep hole, first sleeve lining is provided with locating slot, and locating slot is matched somebody with somebody with ball
Close and action bars and the first sleeve link into an integrated entity action.
8. blake bottle according to claim 1, it is characterised in that the width of the annulus is 1 ~ 1.5cm, under the body
End contacts with bottom wall and is provided with the second sealing gasket.
9. blake bottle according to claim 1, it is characterised in that the bottleneck inner side is provided with the sealing with bottle cap one
Plug, is provided with the passage coordinated with the second support tube in the middle of sealing-plug.
10. blake bottle according to claim 1, it is characterised in that the inside bottom wall face is provided with groove, groove and installs
Fixed block, is provided with axis hole on fixed block, under first support tube end closure and be provided with insertion axis hole rotating shaft.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611195455.9A CN106754369B (en) | 2016-12-22 | 2016-12-22 | Culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611195455.9A CN106754369B (en) | 2016-12-22 | 2016-12-22 | Culture bottle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106754369A true CN106754369A (en) | 2017-05-31 |
| CN106754369B CN106754369B (en) | 2023-06-09 |
Family
ID=58900383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611195455.9A Active CN106754369B (en) | 2016-12-22 | 2016-12-22 | Culture bottle |
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| Country | Link |
|---|---|
| CN (1) | CN106754369B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024754A (en) * | 2019-05-30 | 2019-07-19 | 黑龙江省农业科学院齐齐哈尔分院 | Callosobruchus chinensis supports worm and changes beans device |
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| US5375372A (en) * | 1993-10-22 | 1994-12-27 | Lee; Tso-Cheng | Bicomponent tissue and seedling culture bottle |
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| CN203937942U (en) * | 2014-07-09 | 2014-11-12 | 浙江台州市双瑞塑业有限公司 | Lotion pump |
| CN203999636U (en) * | 2014-07-23 | 2014-12-10 | 中国人民解放军第四军医大学 | A kind of zooblast adherent culture device |
| CN104962460A (en) * | 2015-06-24 | 2015-10-07 | 中山安荞生物科技有限公司 | Biological culture bottle |
| CN205329058U (en) * | 2016-01-22 | 2016-06-22 | 信阳农林学院 | Prevention and cure tea tree antagonistic microorganism culture apparatus for disease |
| CN206319015U (en) * | 2016-12-22 | 2017-07-11 | 青岛农业大学 | Blake bottle |
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| CN110024754A (en) * | 2019-05-30 | 2019-07-19 | 黑龙江省农业科学院齐齐哈尔分院 | Callosobruchus chinensis supports worm and changes beans device |
| CN110024754B (en) * | 2019-05-30 | 2024-02-27 | 黑龙江省农业科学院齐齐哈尔分院 | Green bean weevil insect-raising and bean-changing device |
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