CN106754250B - Inoculating loop placement frame and use method thereof - Google Patents
Inoculating loop placement frame and use method thereof Download PDFInfo
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- CN106754250B CN106754250B CN201710034025.7A CN201710034025A CN106754250B CN 106754250 B CN106754250 B CN 106754250B CN 201710034025 A CN201710034025 A CN 201710034025A CN 106754250 B CN106754250 B CN 106754250B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- General Engineering & Computer Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention relates to an inoculating loop placing frame and a using method thereof, wherein the inoculating loop placing frame comprises a horizontal placing frame used for horizontally placing an inoculating loop, a horizontal frame lifting mechanism is arranged at the bottom of the horizontal placing frame, a through hole is formed in the horizontal placing frame, an inclined placing frame used for obliquely placing the inoculating loop is arranged on one side of the horizontal placing frame, when the inoculating loop is placed on the inclined placing frame, an extension line of a rod body part of the inoculating loop is intersected with the axis of the through hole, and an inclined frame lifting mechanism is arranged at the bottom of the inclined placing frame.
Description
Technical Field
The invention relates to an inoculating loop placing frame and a using method thereof, belonging to the field of bacterial culture.
Background
Before inoculation is carried out by the inoculating loop, a large amount of time is needed to be spent on the burning sterilization of the inoculating loop and the airing after the sterilization is finished, so the whole inoculating period is long and the efficiency is low. Moreover, the incomplete burning sterilization operation can not thoroughly eliminate the sterilization body. When the bacteria on the inoculating loop are more, the direct high-temperature burning can lead the bacteria to be heated and deformed rapidly, aerosol splashing is easy to be caused, the operating platform can be polluted to damage the aseptic operating environment, and even the health of operators is endangered, so that the existing facilities have the defects of unsafe and easy environmental pollution when used for inoculating loop sterilization.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide an inoculating loop placement frame capable of safely and cleanly performing a burning sterilization process and a method of using the same.
In order to achieve the above purpose, the present invention adopts the following technical scheme: an inoculating loop rack, its characterized in that: the inoculating loop comprises a horizontal placing frame used for horizontally placing an inoculating loop, a horizontal frame lifting mechanism is arranged at the bottom of the horizontal placing frame, a through hole is formed in the horizontal placing frame, an inclined placing frame used for obliquely placing the inoculating loop is arranged on one side of the horizontal placing frame, when the inoculating loop is placed on the inclined placing frame, an extension line of a rod body part of the inoculating loop is intersected with an axis of the through hole, and an inclined frame lifting mechanism is arranged at the bottom of the inclined placing frame.
The horizontal shelf comprises a horizontal frame body which is formed by combining three horizontal cross beams and three horizontal longitudinal beams and is shaped like a Chinese character 'tian', two grooves are formed in each horizontal cross beam at intervals, and the grooves on different horizontal cross beams are aligned in the longitudinal direction.
The inclined shelf comprises a frame body which is formed by combining two horizontal longitudinal beams and two inclined beams and is shaped like a Chinese character 'kou', an adjusting longitudinal beam parallel to the horizontal longitudinal beams is arranged on the frame body, two ends of the adjusting longitudinal beam are connected with the inclined beams through nuts, a groove is respectively formed in the adjusting longitudinal beam and the two horizontal longitudinal beams, and the three grooves are located on the same straight line.
The horizontal rack lifting mechanism positioned at the bottom of the horizontal rack and the inclined rack lifting mechanism positioned at the bottom of the inclined rack are telescopic supporting feet, and the horizontal rack and the inclined rack share the two supporting feet.
The inclined shelf is arranged at an included angle of 45 degrees with the horizontal plane, and one side of the inclined shelf, which is close to the horizontal shelf, is lower than the plane where the horizontal shelf is located.
The through hole is arranged at the right center of the horizontal shelf, the inoculating loop is placed in the through hole, and the diameter of the through hole is smaller than that of the inoculating loop handle.
The application method of the inoculating loop placing frame comprises the following steps of:
1) Placing the alcohol lamp right below the through hole in the horizontal shelf;
2) Placing an inoculating loop on the inclined shelf, and adjusting the lifting mechanism of the horizontal shelf to enable the inoculating loop to be positioned near the inner flame of the alcohol lamp for heating;
3) After the thalli on the inoculating loop are heated and dried, the inoculating loop is taken down from the inclined shelf and placed in a through hole of the horizontal shelf, and the lifting mechanism of the horizontal shelf is regulated to enable the inoculating loop to be positioned at the outer flame of the alcohol lamp for burning;
4) Placing the burnt inoculating loop on a horizontal shelf for cooling.
Due to the adoption of the technical scheme, the invention has the following advantages: 1. according to the invention, the inoculating loop is firstly placed on the inclined shelf and the thalli on the inoculating loop is burned by using the inner flame of the alcohol lamp with low temperature, the inoculating loop is placed in the through hole on the horizontal shelf after the thalli are heated and dried, and the thalli on the inoculating loop is burned and sterilized by using the outer flame of the alcohol lamp with high temperature, so that the two steps are sequentially carried out, the thalli are not heated rapidly to form aerosol splash to pollute the operating platform, and the physical health of experimental staff is not damaged. 2. The inoculating loop placement frame provided by the invention is simple in structure and convenient to operate. 3. The horizontal shelf lifting mechanism and the inclined shelf lifting mechanism are telescopic supporting feet, so that the heights of the horizontal shelf and the inclined shelf can be conveniently adjusted according to actual needs. 4. The adjusting longitudinal beam is arranged on the inclined shelf through the nuts, and the position of the adjusting longitudinal beam on the frame body can be adjusted according to the requirements, so that various inoculating loops with different specifications can be placed on the inclined shelf more conveniently. 5. According to the invention, the fungus bodies on the inoculating loop are firstly burned on the inclined shelf by using the inner flame of the alcohol lamp with low temperature, the inoculating loop is placed in the through hole on the horizontal shelf after the fungus bodies are heated and dried, and the fungus bodies on the inoculating loop are burned and sterilized by using the outer flame of the alcohol lamp with high temperature, so that the probability of aerosol splashing and pollution to the workbench is reduced, the success rate of sterilization is improved, and the working efficiency of operators is improved.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples.
As shown in fig. 1, the invention comprises a horizontal shelf 1 for horizontally placing an inoculating loop, a horizontal shelf lifting mechanism is arranged at the bottom of the horizontal shelf 1, a through hole 2 is arranged on the horizontal shelf 1, and an alcohol lamp is placed under the through hole 2 when the burning operation is performed. An inclined shelf 4 for obliquely placing the inoculating loop is arranged on one side of the horizontal shelf 1, when the inoculating loop is placed on the inclined shelf 4, an extension line of a rod body part of the inoculating loop is intersected with the axis of the through hole 2, and an inclined shelf lifting mechanism is arranged at the bottom of the inclined shelf 4.
In the above embodiment, the horizontal shelf 1 includes a horizontal frame body formed by combining three horizontal cross beams 1-1 and three horizontal longitudinal beams 1-2 in a shape of a "field", two grooves 3 are provided at intervals on each horizontal cross beam, and the grooves 3 on different horizontal cross beams are aligned in the longitudinal direction. Three recesses 3 in the same longitudinal direction are used for placing one inoculating loop, so that two inoculating loops can be placed by the horizontal rest 1.
In the above embodiment, the inclined rest 4 includes a frame body formed by combining two horizontal longitudinal beams 4-1 and two inclined beams 4-2 in a shape of a "mouth", an adjusting longitudinal beam parallel to the horizontal longitudinal beam 4-1 is arranged on the frame body, two ends of the adjusting longitudinal beam 4-2 are connected with the inclined beams through nuts, after the nuts are loosened, the position of the adjusting longitudinal beam 4-3 on the frame body can be adjusted, and after the nuts are adjusted, the nuts are screwed. The adjusting longitudinal beam 4-3 and the two horizontal longitudinal beams 4-1 are respectively provided with a groove 3, and the three grooves 3 are positioned on the same straight line and are used for placing an inoculating loop.
In the above embodiment, the horizontal rack lifting mechanism located at the bottom of the horizontal rack 1 and the inclined rack lifting mechanism located at the bottom of the inclined rack 4 are telescopic supporting feet 5, wherein the horizontal rack 1 and the inclined rack 4 share two supporting feet 5.
In the above embodiment, the inclined rest 4 is disposed at an included angle of 45 ° with the horizontal plane, and one side of the inclined rest 4 close to the horizontal rest 1 is lower than the plane on which the horizontal rest 1 is located, and the inoculating loop is placed on the inclined rest 4 when the inoculating loop is subjected to inclined burning.
In the above embodiment, the through hole 2 is arranged at the center of the horizontal shelf 1, the inoculating loop is placed in the through hole 2, the diameter of the through hole 2 is smaller than that of the inoculating loop handle, the inoculating loop handle is clamped in the through hole 2, and the alcohol lamp is arranged below the through hole 2 to burn the inoculating loop.
In the above embodiment, the adjusting longitudinal beam is slidably fixed on the oblique beam through nuts at two ends, and when the nuts are loosened, the position of the adjusting longitudinal beam 4-3 on the frame body can be adjusted, and after the adjustment, the nuts are screwed.
Based on the inoculating loop placing frame, the invention also provides a using method of the inoculating loop placing frame, which comprises the following steps:
1) Placing the alcohol lamp right below the through hole 2 in the horizontal shelf 1;
2) An inoculating loop is placed on the inclined shelf 4, and the lifting mechanism of the inclined shelf is adjusted to enable the inoculating loop to be positioned near the inner flame of the alcohol lamp for heating;
3) After the thalli on the inoculating loop is heated and dried, the inoculating loop is taken down from the inclined shelf 4 and placed in the through hole 2 of the horizontal shelf 1, the lifting mechanism of the horizontal shelf is regulated to enable the inoculating loop to be positioned at the outer flame of the alcohol lamp for burning, and the high temperature is utilized to thoroughly eliminate the thalli;
4) And placing the burnt inoculating loop on a horizontal shelf 1 for cooling.
After step 4) is completed, the second inoculating loop can be placed on the inclined shelf 4 and heated by using the inner flame of the alcohol lamp, and steps 2) to 4) are repeated.
The working principle of the invention is as follows:
the inoculating loop placing frame comprises the inclined placing frame 4, the horizontal placing frame 1 and the through holes 2 on the horizontal placing frame 1, when the inoculating loop is placed on the inclined placing frame 4, the inoculating loop is heated in the inner flame of the alcohol lamp, which is an insufficient burning layer, the temperature is relatively low, and the situation that the thalli are heated rapidly to form aerosol splash so as to pollute the operating platform and harm the health of experimental staff is avoided. After the thalli on the inoculating loop is heated and dried, the inoculating loop is placed in a through hole 2 in a horizontal shelf 1, and the inoculating loop is burnt at high temperature in an outer flame of an alcohol lamp to thoroughly sterilize. The operation accords with the burning and sterilizing standard of the microbial inoculation ring, effectively prevents aerosol from generating, and ensures the operation safety and the aseptic operation effect of a laboratory.
The invention has been described with reference to the above embodiments, but the structure, arrangement and connection of the components are all varied, and on the basis of the technical solutions of the invention, modifications and equivalent changes to the individual components according to the principles of the invention should not be excluded from the scope of the invention.
Claims (5)
1. An inoculating loop placing rack is characterized by comprising a horizontal placing rack for horizontally placing an inoculating loop; the horizontal shelf is provided with a through hole, the through hole is arranged at the right center of the horizontal shelf, and an alcohol lamp can be placed under the through hole; the inoculating loop can be placed in the through hole, and the diameter of the through hole is smaller than that of a handle of the inoculating loop; the bottom of the horizontal shelf is provided with a horizontal shelf lifting mechanism, and the inoculating loop can be positioned near the inner flame of the alcohol lamp for heating by adjusting the horizontal shelf lifting mechanism;
an inclined shelf for obliquely placing the inoculating loop is arranged on one side of the horizontal shelf; the inclined shelf is arranged at an included angle of 45 degrees with the horizontal plane, and one side of the inclined shelf, which is close to the horizontal shelf, is lower than the plane where the horizontal shelf is located; when the inoculating loop is arranged on the inclined shelf, an extension line of a rod body part of the inoculating loop is intersected with the axis of the through hole; the bottom of the inclined shelf is provided with an inclined shelf lifting mechanism, and the inoculating loop can be positioned at the outer flame of the alcohol lamp for burning by adjusting the inclined shelf lifting mechanism. .
2. An inoculating loop holder as defined in claim 1, wherein: the horizontal shelf comprises a horizontal frame body which is formed by combining three horizontal cross beams and three horizontal longitudinal beams and is shaped like a Chinese character 'tian', two grooves are formed in each horizontal cross beam at intervals, and the grooves on different horizontal cross beams are aligned in the longitudinal direction.
3. An inoculating loop holder as defined in claim 1, wherein: the inclined shelf comprises a frame body which is formed by combining two horizontal longitudinal beams and two inclined beams and is shaped like a Chinese character 'kou', an adjusting longitudinal beam parallel to the horizontal longitudinal beams is arranged on the frame body, two ends of the adjusting longitudinal beam are connected with the inclined beams through nuts, a groove is respectively formed in the adjusting longitudinal beam and the two horizontal longitudinal beams, and the three grooves are located on the same straight line.
4. An inoculating loop holder as defined in claim 1, wherein: the horizontal rack lifting mechanism positioned at the bottom of the horizontal rack and the inclined rack lifting mechanism positioned at the bottom of the inclined rack are telescopic supporting feet, and the horizontal rack and the inclined rack share the two supporting feet.
5. A method of using the inoculating loop holder of any of claims 1 to 4, comprising the steps of:
1) Placing the alcohol lamp right below the through hole in the horizontal shelf;
2) Placing an inoculating loop on the inclined shelf, and adjusting the lifting mechanism of the inclined shelf to enable the inoculating loop to be positioned near the inner flame of the alcohol lamp for heating;
3) After the thalli on the inoculating loop are heated and dried, the inoculating loop is taken down from the inclined shelf and placed in a through hole of the horizontal shelf, and the lifting mechanism of the horizontal shelf is regulated to enable the inoculating loop to be positioned at the outer flame of the alcohol lamp for burning;
4) Placing the burnt inoculating loop on a horizontal shelf for cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710034025.7A CN106754250B (en) | 2017-01-18 | 2017-01-18 | Inoculating loop placement frame and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710034025.7A CN106754250B (en) | 2017-01-18 | 2017-01-18 | Inoculating loop placement frame and use method thereof |
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| Publication Number | Publication Date |
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| CN106754250A CN106754250A (en) | 2017-05-31 |
| CN106754250B true CN106754250B (en) | 2023-07-21 |
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| CN201710034025.7A Active CN106754250B (en) | 2017-01-18 | 2017-01-18 | Inoculating loop placement frame and use method thereof |
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| CN106754250A (en) | 2017-05-31 |
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