CN1067531C - Health-care chitosan-chitin food - Google Patents
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Abstract
The present invention relates to a health-care food, particularly to an application of chitosan-chitin on a functional health-care food, and a health-care chitosan-chitin food is provided. The health-care chitosan-chitin food comprises chitosan-chitin powder produced by a method of the present invention and comprises a composition of the chitosan-chitin powder and pumpkin powder tartarian buckwheat crystal and also comprises a composition of chitosan-chitin powder, lecithin, phyteral powder and carotene.
Description
The present invention relates to protective foods, specifically be meant the application of chitosan-chitin on function Sexual health promotion food, health-care chitosan-chitin food promptly is provided.
Protective foods is of a great variety on the market at present, and summarize and get up to mainly contain five big classes: the first kind is general heath food, as green alga, royal jelly, genseng, soya-bean milk, healthy tea, honey, wheat tooth oil, rolled oats etc.Second class is a nature food, as coarse food grain, organic vegetable, sea grass class, fruits, mineral substance.The 3rd class is the hypoglycemic based food, as salt-poor diet, food with low sugar content, low-cholesterol food, newly lose weight the sweet taste material, incubate phosphatide.The 4th class is a nourishing reinforced food, as vitamin-E, Ca and multivitamin, micro-nutrient fortified food.The 5th class is a new type health food, as sports beverages, bacillus bifidus beverage, lactobacillus drink.Above product major part is used for replenishing the shortage of human body to certain nutrition, has only the minority protective foods to belong to functional foodstuff, as ginseng royal jelly and bacillus bifidus breast bacteria beverage etc.Royal jelly has the function that delays senility, but contain certain female hormone, and it is edible to be not suitable for children, and active substance needs preserve in-18 ℃ refrigerator, otherwise volatility.With bacillus bifidus, milk-acid bacteria is the beverages such as SANZHU KOUFUYE of raw material, and the adjusting function of human body is arranged, and improves the function of inside of human body environment, but the main active substances bacillus bifidus, its active being difficult to keeps, and owing to above reason, makes its effect be subjected to very big influence.Therefore being badly in need of the new function Sexual health food resource of exploitation, for this series products, requiring the physicochemical character of its active substance to stablize, do not contain or contain hormone substance less as far as possible, do not have extreme chemical reaction, is pure natural, multi-functional protective foods.Chitosan-chitin can be described as a kind of ideal protective foods resource, the condition that its characteristic conforms is above-mentioned.
The object of the invention is to provide the application of chitosan-chitin on function Sexual health promotion food, and health-care chitosan-chitin food promptly is provided.
Health-care chitosan-chitin food provided by the present invention can be the chitosan-chitin powder that adopts method of the present invention to produce.
Health-care chitosan-chitin food provided by the present invention also can be the chitosan-chitin powder that method of the present invention is produced and the composition of the bitter buckwheat crystalline substance of pumpkin powder, and specifically each composition proportion is (weight percent is represented):
Chitosan-chitin 30%
Pumpkin powder 10%
Bitter buckwheat crystalline substance 60%
The brilliant raw material of wherein bitter buckwheat is the Radix Et Rhizoma Fagopyri Tatarici of height above sea level more than 1800 meters, wears into powder, shells and embryo is separated, and again embryo is worn into powder.
Health-care chitosan-chitin food provided by the present invention can also be chitosan-chitin powder and Yelkin TTS that method of the present invention is produced, and plants the matter end, the composition of carotene, and specifically each composition proportion is (weight percent is represented):
Chitosan-chitin 90%
Yelkin TTS 5%
Plant matter end 3%
Carotene 2%
The production method of chitosan provided by the present invention-chitin powder comprises: select ordorlessly, the crab leg in the red deep-sea of do not have rotting cleans up, oven dry.At room temperature handled 22 hours with 4%HCl solution then, make inorganic salt such as contained lime carbonate and calcium phosphate be converted into calcium chloride and remove.After the filtration, until raffinate acid, it is neutral that the alkali detection paper is with the soft water washing.NaOH solution-treated with excessive 10% 4 hours, temperature is controlled at 93 ± 2 ℃, removes deproteinize, fat.Filtration to neutral, afterwards with an amount of absolute ethanol washing, is drying to obtain the elaboration chitin with the soft water washing., survey its deacetylation by acid base titration and see whether reach more than 80%, generally be controlled at 90% 143 ℃ ± 2 ℃ heating 15 hours with 50%NaOH, reaction terminating filters, and is washed till neutrality with soft water, can get chitosan-chitin after the drying.Pulverize (on the stainless steel mill) and sieve and make it 100% by 80 orders, viscosity controller is at 70 ± 5mpa.s, sterilization.
Chitin and chitosan are that chitosan is to belong to the polyose animal fibre, are a kind of rising natural macromolecule amyloses, use very extensively, have showed wide application prospect in fields such as doctor, farming, light industry, chemical industry, weaving, environmental protection.China has also obtained some achievements at present, and " Hai Fukang " artificial skin of developing as Qingdao Marine University has obtained 37 eureka world inventions gold medals, country's 91 annual invention third prizes.The Chinese Academy of Sciences and the cooperation of The 2nd Army Medical College of PLA successfully extract this material from silkworm chrysalis, develop medical sewing thread, wound hemostasis film etc." the chitin artificial skin " of China Textile Science Inst. obtains national inventing patent.But chitosan-chitin directly is processed into protective foods still belongs to blank.The experiment proved that this material has no side effect, good with the tissue intermiscibility, physico-chemical property is stable, pharmaceutical use is preferably arranged, have enhance immunity power, broad-spectrum is antibacterial, promotes organization healing, hemostatic analgesia suppresses the tumour cell diffusion, and plurality of health care functions such as delay senility.Therefore pass through animal and human's body repetition test among the present invention, determine the best physical and chemical index scope of chitosan-chitin, and develop serial health-care chitosan-chitin food on this basis as function Sexual health promotion food.
1, the main physical and chemical of chitin-chitosan is:
1. viscosity: pure chitosan is a kind of white or translucent sheet of canescence or pulverulent solids; nothing is smelt, nontoxicity; the chitin molecular weight is about 1,000,000-2,000,000 in the organism; the back chitin molecular weight of purifying is about 300,000-700,000; chitosan molecular weight by chitin after deacetylated is then lower, is about 20-50 ten thousand.The molecular weight size of chitin and chitosan is often represented with their viscosity numerical value just.
2. degree of deacetylation: have strong hydrogen bond action between stable ring texture and the macromole owing to have in the chitin macromole, make them have stable physics, chemical property.Not fusion (200 ℃ of decomposition) is water insoluble again, and in diluted acid, diluted alkaline and the common organic solvents, chitin can dissolve in strong acid (vitriol oil, hydrochloric acid, nitric acid, 85% phosphoric acid etc.), but the degraded that chain takes place simultaneously obviously reduces molecular weight.The chitosan macromole is owing to ethanoyl has removed in a large number-NH
2Exist, make its solubility property be better than chitin greatly.It can be dissolved in most diluted acid, in (as formic acid, acetate, naphthenic acid, phenylformic acid etc.) and make uniform chitosan solution.Also active big because of chitosan, thus its dilute acid soln at room temperature viscosity reduce gradually, complete hydrolysis becomes glucosamine at last.
On chitin and chitosan macromolecular structure, they have the structure similar to vegetable fibre, all have an acetamido or amino to make chitin-chitosan have the structure of similar human body osso-albumin tissue on their macromolecular each repeating units.Their this dual structure has been given their fabulous biological medicine characteristics.Make they and human body have fabulous Bc.Can be decomposed by the intravital N,O-Diacetylmuramidase of biology and absorb, say that therefore the degree of deacetylation (being amino content) in the chitosan chitin is the main foundation of its health-care effect of decision.
3. granularity: the chitosan-chitin after the purification is fibrous should the pulverizing and grinds to form certain tiny particle, experiment showed, the more little absorption of human body that helps more of granularity repeatedly through us.
2, chitosan-chitinous extraction process
A kind of polysaccharide that chitin (Chitine) is a kind of straight-chain polymer of like fibrous element, formed by the N-acetylglucosamine monomer polymerization of similar glucose.The N-acetylglucosamine, chitin, chitosan, Mierocrystalline cellulose, the molecular configuration of glucose is as follows.Chitin is poly-smart as can be known is the product of chitin lose ethanoyl after the deacetylation effect after from molecular structure; it goes the acetyl degree is 65%-99%; because it is deacetylated fully still to have no idea in the world so far, so the product after deacetylated is commonly referred to chitosan-chitin (Chitosan-Chitin) (because of there are two kinds of compositions simultaneously in product) with chitin.
Chitin is commonly called as chitin, chitin, be that crustacean (as crab, shrimp) shell, insect (as cockroach, xylotrupes dichotomus) epidermis, mollusk (as cuttlefish, shellfish) organ, mushroom are (as mushroom, lichens) important composition of cell walls, nearly 1,000 hundred million tons at occurring in nature
The abundant Mierocrystalline cellulose that is only second to the land of resource.Chitin extracts more convenient from shrimp, crab shell in general, mainly contain three kinds of compositions in their crust, inorganic salt such as lime carbonate and calcium phosphate account for 45%, crude protein and fat account for 27%, chitin accounts for about 20%, chitinous content in all kinds of crusts, in the shrimp shell, account for 15-30%, account for 15-20% in the crab shell, mainly form by producing chitin in shrimp, the crab shell by two portions technology, remove lime carbonate and remove protein with hot diluted alkaline with dilute hydrochloric acid, the chitin of making has just been made chitosan again after concentrated base takes off acetyl.Conventional at present extracting method is: add rare NaOH in the crab shell to remove deproteinize, grind, add sulfurous acid with deliming, filter washing, drying.But, this preparation method's product is technical grade product roughing preparation method, can not be edible as food grade products, because the product ash oontent height of preparing like this (general ash oontent all＞5%), and heavy metal content is also higher, problem according to above existence, a large amount of research work have been done among the present invention, slave unit is to preparation technology, all improve, test of many times is groped the colory chitin of preparation food grade, chitosan best equipment and technology finally, we prepare chitin, the chitosan raw material mainly is a deep-sea crab shell, and its technical process is as follows:
Select the crab shell, add soft water and wash, add HCl and carry out acid treatment, add soft water and wash, add the NaOH soda boiling, add KMnO
4And the oxalic acid decolouring, drying adds dense NaOH and boils to take off acetyl, adds soft water and washes, and drying obtains chitosan and chitin mixture.
Concrete grammar is: the crab shell is selected cleaned up, oven dry, with the HCl (the reagent utmost point) of excessive 2mol solution reaction 4-5 hour, filter, at room temperature handled 20-24 hour with the HCl solution of 2mol again, make contained lime carbonate be converted into calcium chloride, remove, filter, the soft water washing is to neutral, with the NaOH solution-treated of excessive 5-10% 3-4 hour, temperature 90-95 ℃, remove deproteinize, can remove for several times remaining inorganic salt and protein such as lime carbonate repeatedly, 0.5%, stirred dipping one hour in the potassium permanganate again, soft water is washed the back and stir decolouring in 30-40 minute in 60-70 ℃ in 1% oxalic acid, through fully washing, with an amount of absolute ethanol washing, drying just can make yellow elaboration chitin at last, the chitin that last method makes heats 10-18 hour twice in 140 ℃ with 50% NaOH, filter then, to neutral, can get the white solid chitosan after drying with the soft water washing.Chitosan with method for preparing is superior in quality, meets food grade fully, and through small white mouse toxicological experiment active substance stability test, hygiology detects and the nourishing function test, all meets the standard of the new protective foods of Ministry of Health's formulation.
On above basis, we add other pure natural substance in chitosan-chitin, as pumpkin powder, Yelkin TTS, Radix Et Rhizoma Fagopyri Tatarici etc., and preparation chitosan chitin protective foods.
3, chitosan-chitinous main physical and chemical index and the relation of regulating immunologic function:
Nineteen sixty Nowell finds phytohaemagglutinin (Phytohemagylutin, PHA) and lymphocyte mix, carry out vitro culture, can see after a few days that the vigorous phenomenon of division appears in significantly large-scale parent cell, again find that the synthetic of lymphocytic protein and nucleic acid also increased when following morphological change thereafter.The lymph of PHA stimulation that Here it is transforms phenomenon.After prove tuberculin again in succession, the reacting positive body is a lymphocyte adding tuberculin, also occurs transforming phenomenon during vitro culture.When in the organ transplantation research work, finding the lymphocyte mixed culture of donor and acceptor, the combination that transformation efficiency is low, then the internal organs survival time of Yi Zhiing is long.All can the lymphocyte transformation phenomenon appear external from tuberculin reaction and immunclogical response of transplantation, so think that the cellular immunity of LTR and body has substantial connection.Lymphocyte transformation test at present has been widely used in the mensuration of body cell immunologic function.This test is tested with this method:
(1) material:
1. heparin preservative-free heparin injection 50 μ g/ml blood, or 0.4% rough hepar siccatum immersion liquid 0.1ml/ml blood.
2. 199 nutritive mediums contain foetal calf serum 15%, penicillin 100 μ g/ml, Streptomycin sulphate 100 μ g/ml.
3. chitosan-chitin: 0.5% acetum.
A. viscosity is 200mpa.s, 180mpa.s, 160mpa.s, 140mpa.s, 120mpa.s, 100mpa.s, 80mpa.s, 60mpa.s, 40mpa.s, (0.5%) 20 ℃
B. degree of deacetylation is 60% 70% 80% 90%
C. granularity is 60 orders, 70 orders, 80 orders, 90 orders
4. Hanks liquid
2, method
1. it is heavy even that white corpuscle culture method, extracting vein blood 1ml are put into calparine pipe, puts in 37 ℃ of incubators and leave standstill half an hour, treat that red corpuscle sinks, the partly about 0.5ml of leukocytic blood plasma is rich on the sucking-off upper strata, puts into the culturing bottle that the 3ml199 nutritive medium is housed, and adds corresponding chitosan-chitin solution again.Shake up back jam-pack soft rubber ball, put 37 ℃ of incubators, cultivated 72 hours.
2. smear staining method: cultivate when finishing, cell hang, move in the test tube, centrifugal 1000rpm 10min is sink to the pipe end with cell, and supernatant liquor is to the greatest extent treated that the residual small amount of liquid of tube wall is back to manage at the end, makes push jack with sharp suction pipe absorption cell.Dry back methyl alcohol is fixed, and dyes with auspiciousization staining.
3. sentence read result method: the size of the outstanding cell of the morphological criteria of interpretation parent cell, the ratio of nuclear and cell space, the dyeability of born of the same parents' dress, having or not of the structure of nuclear and kernel is divided into following four kinds of cells.
A. sophisticated small lymphocyte: the same 6-8 μ of lymphocyte when not cultivating, the karyomit(e) densification of nuclear, nuclear is big with the ratio of cell space, and endochylema dyeing is slight basophilia.
B. the lymphocyte of: bigger than small lymphocyte, about 8-12 μ, caryoplasm are fine and close too, and kernel occurs, and the endochylema dyeability is the same with above-mentioned cell.
C. lymphoblast: cell individual increases, about 12-20 μ, and form is irregular, and pimple is often arranged, and it is big that nuclear becomes, and kernel obviously appears in chromatin mercapto pine, and endochylema broadens, and nuclear diminishes with the cell space ratio, and endochylema is obviously basophilia by force, the endochylema cavity often occurs.
D. other cell: neutrophil leucocyte is after cultivating 72 hours, and overwhelming majority decay or death are fragment, however eosinophilic granulocyte after cultivating 72 hours, the overwhelming majority decays into death and is fragment.
Above-mentioned a, b item are no transformed cells, and the c item is a transformant, differentiate in the classification that main points are: the having or not of the structure of nuclear, particularly kernel.
4. the calculating of lymphocyte transformation rate: 200 lymphocytes of counting under high power or oily mirror
(3) result:
1. viscosity is to the influence (seeing Table 1) of lymphocyte transformation rate
Table 1 chitosan-chitinous viscosity is to the influence of lymphocyte transformation rate
| Viscosity mpa.s | 200 | 180 | 160 | 140 | 120 | 100 | 80 | 60 | 40 | CK |
| Transformation efficiency % | 13.4 | 19.5 | 30.2 | 47.8 | 48.1 | 51.3 | 71.9 | 80.5 | 80 | 11.5 |
Annotate: 1. data are three revision test average datas in the table
2. chitosan-chitinous deacetylation is 91% in the table, and granularity is 80 orders.
(2) degree of deacetylation sees Table 2 to the influence of mouse spleen lymphocyte transformation efficiency
Table 2 chitosan-chitin deacetylation is to the influence of lymphocyte transformation rate
| Degree of deacetylation % | 60 | 70 | 80 | 90 | CK |
| Transformation efficiency % | 34.9 | 41.2 | 68.9 | 74.3 | 10.5 |
Annotate: 1. data are three repetition average datas in the table
2. the chitinous viscosity of chitosan is 80 orders for the 75mpa.s granularity in the table
(3) granularity is to lymphocyte transformation rate influence (seeing Table 3)
Table 3 granularity influences lymphocyte transformation rate
| Granularity (order) | 60 | 70 | 80 | 100 | CK |
| Transformation efficiency % | 51.4 | 53.7 | 72.1 | 79.7 | 11.4 |
Annotate: 1. data are three revision test average datas in the table
2. chitosan-chitinous deacetylation is 91% in the table, and viscosity is 75mpa.s
More than in three tables CK be blank, promptly add chitosan-chitin.
Show from above data
(1) chitosan-chitinous viscosity transformation efficiency less than 100mpa.s the time obviously improves, this be explanation to be tried the thing molecular weight more little, its effect of regulating immunity function is good more.
(2) chitosan-chitinous deacetylation is 80% when above, and lymphocyte transformation rate is significantly improved, and illustrates that free amine group plays an important role regulating on the immunity function.
(3) chitosan-chitinous granularity is when 80 orders are above, and lymphocyte transformation rate improves, and illustrates that granularity is more little, is convenient to animal body and absorbs, and its immunomodulatory effect is good more.
4, the relation between chitosan-chitin and the macrophage phagocytic function
The intravital small lymphocyte of scavenger cell and machine all is to participate in immunoreactive main cell composition, and scavenger cell is being handled antigen and at important anti-infective of specific antibody and the collaborative performance down of lymphocyte, antitumor action.Some immunostimulant in addition, the function of the stimulation scavenger cell that immunological adjuvant is special, observation is in the particularly variation of malignant tumor patient of various diseases, objective indicator to therapeutic response, in human trial, be difficult for obtaining the scavenger cell for test of capacity, adopt the foaming of Chinese medicine Chinese blister beetle alcohol leach liquor can obtain result preferably at present, suitcase liquid and convenient sources, the chicken hemocyte that is easy to observe are at incubated in vitro, get cell and make smear, microscopy calculates to be engulfed percentage ratio and compares with normal value with phagocytic index, can estimate the phagocytic function of patient's scavenger cell.
(1) material
1. 10% Chinese blister beetle leach liquor, 10g Chinese medicine Chinese blister beetle are dipped in 100ml 95% alcohol port grinding bottle jam-pack, and to put refrigerator standby.
2. chicken cell suspension, the wing vein is got blood, and heparin 50ug/ml anti-freezing is put into Alsere ' preservation liquid with 1/10 ratio and was preserved one month, gives a baby a bath on the third day after its birth inferiorly with physiological saline with preceding, and it is standby to be made into 5% concentration.
3. tried thing: chitosan-chitin (for this case is extracted, viscosity: 75mpa.s, deacetylation: 91%, granularity 80 orders).
4. the animal subject small white mouse is divided four groups
I group: make it suffer from skin cancer, the feed (T that feeds and add chitosan
+)
II group: make it suffer from skin cancer, the feed (T that feeds and add chitosan
-)
III group: normal mice, the feed (CK that feeds and add chitosan
+)
IV group: normal mice, the feed (CK that feeds and do not add chitosan
-)
(2) method:
Get the Intraabdominal scavenger cell of animal subject, add the mixing that vibrates gently, put into 37 ℃ of water-baths through 5% chicken red blood cell 0.02ml of washing.Incubation 30 minutes, shook once in per therebetween 10 minutes, centrifugal, getting the sediment smear fixes, also can in the incubation process, add the cover plate bar, two opening and closing also, put into above-mentioned chicken red blood cell and expanding foam solution mixed solution test tube, scavenger cell is swashed along slide, after incubation finishes, the tegillum slide is taken out fixing, with the dyeing of Ji's nurse Sa (Ji's nurse Sa dye liquor is done 1/10 dilution with PH6 or 9, phosphoric acid buffer), dying 4-5 minute is flushable mounting with smear or the cover plate that is attached with scavenger cell, engulf the result with high power or oily spectroscopy, with following two index expression activate the phagocytic capacity:
A. percentage phagocytosis: the scavenger cell number that promptly gulps down chicken red blood cell in each hundred scavenger cell.
B. the number difference of each macrophage phagocytic chicken red blood cell (from zero to tens) 100 scavenger cells are made the chicken red blood cell that gulps down summation divided by 100, promptly get the scavenger cell index, i.e. the mean number of each macrophage phagocytic chicken red blood cell.Think that according to treatment and prevention of tumour institute of Chinese Academy of Medical Sciences test-results above-mentioned binomial index often is parallel, so only can reflect its activate the phagocytic capacity with percentage phagocytosis.
(3) result's (seeing Table 4)
The relation of table 4 chitosan and macrophage phagocytic function
| Group | Sample number | Activate the phagocytic capacity (%) |
| T + | 30 | 56.5 |
| T - | 30 | 39.3 |
| CK + | 30 | 69.4 |
| CK - | 30 | 62.7 |
Annotate: T
+Suffer from the knurl mouse and feed chitosan
T
-Suffer from the knurl mouse and do not feed chitosan
CK
+Normal mice is fed chitosan
CK
-Normal mice is not fed chitosan
From interpretation of result, the huge cytophagy activity of biting of trouble cancer mouse is minimum to be 39.3%.Bring up to 56.3% but suffer from the cancer mouse activate the phagocytic capacity of having fed behind the chitosan, normal mice is fed and has also been improved than the normal mice of not feeding chitosan behind the chitosan.Illustrate that chitosan-chitin has the activated effect to the phagocytic activity of scavenger cell.Thereby infer that the chitosan chitin can be regulated immunity function, have the function that suppresses tumour simultaneously.Result of study can be inferred DAC-70 via T-cell system activated macrophage (M φ), and may be by the B-cell system by the CM-chitin.
Protective foods provided by the present invention has been determined the best-of-breed technology indication range of chitosan chitin as new functional food, and its health-care effect will be enhanced.Except the function of above-mentioned adjusting immunity function, also will improve people's functions of intestines and stomach reducing fat sterol, hypotensive, hypoglycemic, remove harmful toxins in the human body, as heavy metal, agricultural chemicals etc., certain effect will be arranged.Below we are analyzed integral part one by one
1, regulates immunologic function (top existing detailed description)
(1) material
1. tried thing: chitosan-chitin
2. animal subject: exempt from son and divide two groups
The I high cholesterol diet of feeding does not add chitosan in the feed
The II high cholesterol diet of feeding, chitosan (2%) in addition in the feed
(2) method
After feeding one month, content of cholesterol in the test blood.
(3) result and analysis
Group I serum cholesterol value is up to 629mg;
Group II serum cholesterol value is 287mg.
This result illustrates that chitosan-chitin has the function that suppresses cholesterol.We know that cholesterol is the essential substance that constitutes human body midbrain, nerve, sexual hormoue, cytolemma etc., being used to digest and assimilate fatty bile acide also is that cholesterol is transformed, but when cholesterol surpasses normal value, can hoard in vessel wall, blood circulation is worsened, cause coronary heart disease, arteriosclerotic diseases such as myocardial infarction.Chitosan-chitin is to the inhibiting mechanism of cholesterol, tentatively be inferred as and prevent that 1. cholesterol from absorbing in vivo, chitosan is easy to the bile acide combination and all excretes, owing to lack the effect that bile acid has just suppressed enzyme, so cholesterol can't be transformed into the cholesterol ester that is easily absorbed by intestinal tube, thereby reduced cholesterol; 2. the absorption of overslaugh fat, chitosan contains the positively charged ion of positively charged, accumulates in vivo around the electronegative oil droplet, form barrier and the overslaugh absorption, fat in the blood is present in cholesterol and the proteinic particle, so along with the minimizing of blood fat fat, cholesterol level also can reduce thereupon.3. impel cholesterol to transform, cholesterol is an important component in the Digestive system that generated of liver inner cholesterol, certain reserves are arranged in gall-bladder, bile acide digests in perfect aspect usually, after absorbing the task of fat, absorb again and get back in the gall-bladder by small intestine, because of chitosan easily and bile acide in conjunction with also all excreting, in order to keep that a certain amount of bile acide deposit is arranged in the gall-bladder, cholesterol level must descend in the bile acide blood thereby just must be in liver cholesterol be changed into.
2, hypotensive
Vasoconstriction conversion enzyme (ACE) causes hypertensive major cause, the present invention is to contain high sodium-chlor, high sodium salt and high chlorine feed are fed normal mouse, found that, only the feed mouse increased blood pressure of feed of high sodium-chlor adds chitosan (deacetylation 91%, granularity 80 orders of our preparation then at feed, viscosity 70mpa.s), because the NH that dissociates in the chitosan
2 +Effect, impel the Cl ionic to drain, because activity and the Cl ionic relationships of ACE are close.Make the active reduction of ACE so the Cl ionic reduces, suppressed the rising of blood pressure.
3, hypoglycemic
Have in the human body and make blood glucose value keep certain robot control system(RCS), this is that many hormones all can make blood glucose value rise because the participation of various hormones is arranged, but has only Regular Insulin that blood glucose value is descended.If therefore pancreatic secretion Regular Insulin ability drop, or the situation of soma worsens, in the time of can't making full use of Regular Insulin, blood glucose value all can raise gradually.As everyone knows, in the prevention and treatment for diabetes, foodstuff fibre is very effective.And chitosan-chitin is the polycation fiber, and hypoglycemic function is arranged equally, and experimentation on animals has confirmed this point.
Respectively with containing chitosan-chitinous feed, the feed of general vegetable fibre and the feed that does not contain any fiber, feed the mouse that has suffered from diabetes, the result eats the urine sugar value of the mouse that contains chitosan-chitin feed and blood glucose value is all low than other two groups on an empty stomach.Illustrate that chitosan-chitin that we prepare has the effect that suppresses rising to blood sugar.
4, improve gastrointestinal function, improve constipation
Foodstuff fibre has the promotion enterogastric peristalsis, improve the effect of Digestive tract, have the function of general foodstuff fibre equally, also find in the experiment as the chitosan-chitin of polycation fiber, youngster's fourth glycan also can promote the propagation of beneficial bacteria in the intestines, and this is that general foodstuff fibre is unexistent.Fact proved, after a lot of people have eaten the chitosan chitin of our preparation, gastrointestinal system improves greatly, the stool of long-term diarrhea is shaped, the defecation of constipation is unobstructed, the people who suffers from various chronic gastropathies has obtained improvement in various degree, generally feels better than the SANZHU KOUFUYE of selling on the market now.This mainly is that the active ingredient stability of chitosan is fine, and the activity of the main active substances-bacillus bifidus in the SANZHU KOUFUYE is difficult to keep.
5, absorption objectionable impurities such as heavy metal and discharge the body place
Along with the progress of science and technology, also bring a lot of public hazards, cause human a lot of diseases that take place, what wherein be widely known by the people most is the heavy metal public hazards.Experiment shows that chitosan-chitin is owing to its specific molecule structure, and adsorbable heavy metal adds mercuric chloride solution (flow velocity 0.5ml/min) in filling up the pipe of chitosan.Test via the SP-9 atomic absorption spectrum, find that beginning absorption speed is higher, after about 2 hours, reach an equilibrium value, chitosan-chitin can be considered to be poromerics, and when the heterogeneous body polymkeric substance was lower than T8, the thermal motion meeting of key section was restricted and forms the molecule irregular stacking, so form micropore small molecules or ion are passed through, adsorption process roughly was divided into for three steps.Be that adsorbate moves on the adsorbent, and then diffusion, finally reach absorption.Find that in addition take off more height of acetyl degree, then adsorptive power is stronger, this is the decision of metal ion-chelant process kinetics.The mankind of this function under living in the environmental pollution serious condition now is very important.
6, improvement is had a pain in the back
The traditional Chinese medical science is paid attention to tip round-robin physiology very much.So hemostasis is arranged, the notion of water poison.Hemostasis is exactly the not smooth morbid state of gas, blood, water, during hemostasis tip circulation bad, shell temperature reduces, and becomes the disease of being afraid of cold because pair cell, muscle oxygen, the nutrient supply deficiency is so can cause and have a pain in the back.Just can improve the symptom of having a pain in the back as long as can expand the volume of blood flow of arteriole.But chitosan is the degradation production vagus nerve stimulation in vivo, makes the arteriole expansion via the excitement of autonomic nerve maincenter, has a pain in the back thereby improve.
Embodiment 1
Select ordorlessly, the crab leg in the red deep-sea of do not have rotting cleans up, oven dry.At room temperature handled 22 hours with 4%HCl solution then, make inorganic salt such as contained lime carbonate and calcium phosphate be converted into calcium chloride and remove.After the filtration, until raffinate acid, it is neutral that the alkali detection paper is with the soft water washing.Na0H solution-treated with excessive 10% 4 hours, temperature is controlled at 93 ± 2 ℃, removes deproteinize, fat.Filtration to neutral, afterwards with an amount of absolute ethanol washing, is drying to obtain the elaboration chitin with the soft water washing.Whether, survey its deacetylation by acid base titration and reach more than 80% 143 ℃ ± 2 ℃ heating 15 hours with 50%NaOH, generally be controlled at 90%, reaction terminating filters, and is washed till neutrality with soft water, can get chitosan-chitin after the drying.Pulverize (on the stainless steel mill) and sieve and make it 100% by 80 orders, viscosity controller is at 70 ± 5mpa.s.Sterilization.
With these product to animal experiment and human experiment.All demonstrate great health care function.
Embodiment 2
With the chitosan-chitin powder of preparation among the embodiment 1, mix the bitter growing the grain of pumpkin powder, specifically each composition proportion is (weight percent is represented):
Chitosan-chitin 30%
Pumpkin powder 10%
Bitter buckwheat crystalline substance 60%
* the brilliant raw material of bitter buckwheat is the Radix Et Rhizoma Fagopyri Tatarici of height above sea level more than 1800 meters, wears into powder, shells and embryo is separated, and again embryo is worn into powder.
The functional food major function of making of more than filling a prescription is hypoglycemic, and reducing blood-fat is the special-purpose functional food of diabetics.
Embodiment 3
With the chitosan-chitin powder of preparation among the embodiment 1, additional Yelkin TTS is planted the matter end, carotene.Concrete each composition proportion is (weight percent is represented):
Chitosan-chitin 90%
Yelkin TTS 5%
Plant matter end 3%
Carotene 2%
The functional food of this formulated will have better curative effect to cardiovascular and cerebrovascular patients such as hypertension, hyperlipidemia, hypercholesterolemia, arteriosclerosis, thrombus.
Give nearly 50 people's test-meals with made chitosan-chitin, generally reflection is good, lifts several examples below:
1. certain woman: 35 years old, it was swollen to suffer from the ovary bundle, edible chitosan-chitin powder, and every day, 6g after two months, constipation occurred.Continue to eat, week back constipation disappears, and has reduced about 1cm to the examination in hospital Ovarian Tumor.
2. certain woman is 47 years old, suffers from chronic gastritis for a long time, and eat chitosan chitin powder 2g every day, and after two months, stomach is flatulence no longer, pain, and appetite increases.
3. certain man is 57 years old, suffers from hypertension, hyperlipidemia, takes chitosan-chitin, every day 6g, after three months, blood pressure is basicly stable, and cholesterol is reduced to 195mg% by 238mg%, beta lipoprotein is reduced to 728mg% by 928mg%, and triglyceride is reduced to 269mg% by 445mg%.
4. certain man is 71 years old, suffers from late gastric cancer, hand formula not, and the doctor says maximum work 3 months.Edible chitosan-chitin, 6g-10g/ day every day, in high spirits after two months, appetite increases, and competent slight housework has repeatedly during this, but patient does not feel pain, survives half a year.
5. in addition, there are 5 examination trenchermans to find on one's body, face, the thrust of the mole of under-arm and monkey and so on, hardening is before this dwindled, and comes off then.
6. certain man, 51 years old, suffer from diabetes, edible chitosan chitin 6g every day, after two months, fasting plasma glucose drops to 104mg% by 146.2mg%, and urine becomes (-) by+1, glucose in urine by+4 become+2, after four months, spirit is clearly better, and continuous three months fasting plasma glucoses are at 74-86mg%, glucose in urine (-).
Claims (2)
1. health-care chitosan-chitin food, it is characterized in that adopting following method to produce chitosan-chitin powder: to select in the deep-sea ordorless, do not have and rot, red crab leg cleans up, oven dry, at room temperature handled 22 hours with 4%HCl solution then, making contained lime carbonate and calcium phosphate inorganic salt be converted into calcium chloride removes, filtering the back uses sour with the soft water washing until raffinate, it is neutral that the alkali detection paper is, NaOH solution-treated with excessive 10% 4 hours, temperature is controlled at 93 ± 2 ℃, remove deproteinize, fat, filtration is extremely neutral with the soft water washing, afterwards with an amount of absolute ethanol washing, be drying to obtain the elaboration chitin, heated 15 hours at 143 ℃ ± 2 ℃ with 50%NaOH, survey its deacetylation by acid base titration and see whether reach more than 80%, generally be controlled at 90%, reaction terminating filters, be washed till neutrality with soft water, can get chitosan-chitin after the drying, pulverizing on stainless steel mill and sieving makes it 100% by 80 orders, viscosity controller is at 70 ± 5mpa.s, after sterilization promptly obtains required chitosan-chitin powder.
2. the described health-care chitosan-chitin food of claim 1, it is characterized in that forming by chitosan-chitin powder and pumpkin powder, bitter buckwheat crystalline substance, each composition weight percentage: chitosan-chitin 30%, pumpkin powder 10%, bitter buckwheat crystalline substance 60%, the brilliant raw material of described bitter buckwheat is the Radix Et Rhizoma Fagopyri Tatarici of height above sea level more than 1800 meters, wears into powder, shell and embryo is separated, again embryo is worn into powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100243A CN1067531C (en) | 1997-01-13 | 1997-01-13 | Health-care chitosan-chitin food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100243A CN1067531C (en) | 1997-01-13 | 1997-01-13 | Health-care chitosan-chitin food |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1187948A CN1187948A (en) | 1998-07-22 |
| CN1067531C true CN1067531C (en) | 2001-06-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97100243A Expired - Fee Related CN1067531C (en) | 1997-01-13 | 1997-01-13 | Health-care chitosan-chitin food |
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| Country | Link |
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| CN (1) | CN1067531C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1247819A1 (en) * | 2001-04-04 | 2002-10-09 | Dainichiseika Color & Chemicals Mfg. Co. Ltd. | Purified chitins and production process thereof |
| CN100491406C (en) * | 2006-11-24 | 2009-05-27 | 暨南大学 | Phosphoryl choline chitosan derivative synthesis method |
| CN104256246A (en) * | 2014-07-25 | 2015-01-07 | 湖北亿龙源食品有限公司 | Donkey-hide gelatin brown sugar and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU1821471C (en) * | 1990-12-06 | 1993-06-15 | Дальневосточный технический институт рыбной промышленности и хозяйства | Process for preparing chitosan |
| JPH05279401A (en) * | 1992-03-31 | 1993-10-26 | Nippon Kayaku Co Ltd | Production of powdery chitosan |
| JPH06181725A (en) * | 1992-12-17 | 1994-07-05 | Kiteii:Kk | Agent for solidifying easily oxidizable oily substance |
| JPH07135923A (en) * | 1993-11-19 | 1995-05-30 | Takeo Tanabe | Composition for functional food |
-
1997
- 1997-01-13 CN CN97100243A patent/CN1067531C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU1821471C (en) * | 1990-12-06 | 1993-06-15 | Дальневосточный технический институт рыбной промышленности и хозяйства | Process for preparing chitosan |
| JPH05279401A (en) * | 1992-03-31 | 1993-10-26 | Nippon Kayaku Co Ltd | Production of powdery chitosan |
| JPH06181725A (en) * | 1992-12-17 | 1994-07-05 | Kiteii:Kk | Agent for solidifying easily oxidizable oily substance |
| JPH07135923A (en) * | 1993-11-19 | 1995-05-30 | Takeo Tanabe | Composition for functional food |
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| Publication number | Publication date |
|---|---|
| CN1187948A (en) | 1998-07-22 |
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