CN106750399B - A kind of selfreparing, photaesthesia aquagel and preparation method thereof - Google Patents
A kind of selfreparing, photaesthesia aquagel and preparation method thereof Download PDFInfo
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- CN106750399B CN106750399B CN201611102643.2A CN201611102643A CN106750399B CN 106750399 B CN106750399 B CN 106750399B CN 201611102643 A CN201611102643 A CN 201611102643A CN 106750399 B CN106750399 B CN 106750399B
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
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- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0031—Hydrogels or hydrocolloids
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
Abstract
The present invention discloses a kind of selfreparing, photaesthesia aquagel and preparation method thereof; the preparation method of the aquagel includes: (1) using carboxymethyl chitosan and nitro dopamine as raw material progress acylation reaction, obtains the chitosan derivatives of the amine groups of DOPA containing nitro；(2) by the aqueous solution and Fe of the chitosan derivatives of the amine groups of DOPA containing nitro3+Solution mixing, adjusts the pH to 6.5~9.5 of mixed solution, obtains selfreparing, photaesthesia aquagel.The present invention uses mussel biomimetic chemistry method, and by crosslinking the chitosan derivatives for containing nitro DOPA amine groups with metallic ion coordination, dopamine has fine biocompatibility, reduces toxicity of the hydrogel to organism of conventional method preparation；Selfreparing obtained, photaesthesia aquagel adhesion is strong, compliance is good, space-time controllability is strong, as wound dressing, can maintain best moist environment for a long time on wound face, promote wound epithelialization, accelerating wound healing.
Description
Technical field
The present invention relates to a kind of hydrogel and preparation method thereof, in particular to a kind of selfreparing, photaesthesia chitosan water-setting
Glue and preparation method thereof.
Background technique
Chitosan is that unique positive linear polysaccharide, basic unit are gucosamine in nature, has bio-compatible
Property it is good, have no toxic side effect, abundance the features such as, oneself food industry, synthesis of pharmaceutical preparation, medical macromolecular materials etc. lead
Domain obtains extensive development and application.Based on different mechanism (covalently, ion, hydrogen bond), chitosan can form hydrogel, this is
There are many relevant reports.Chitosan and glutaraldehyde etc. can form chemical gel, can also be with thioacetic acid, acrylic acid, grass
Acid etc. forms physical gel.In addition chitosan and metal ion or other biological polymer (such as: going back virgin rubber, carragheen etc.)
Forming ionic cross-linked bond by electrostatic interaction becomes gel.However, the formation of these aquagels is both needed to additionally incorporate
Crosslinking agent such as glutaraldehyde etc., the use of crosslinking agent have the following deficiencies: that (1) is crosslinked due to glutaraldehyde and amino on chitosan
It reacts extremely rapid, glutaraldehyde is caused to be difficult to be uniformly mixed with chitosan aqueous solution；(2) since solution viscosity is very big, it is difficult to
System cross-linking reaction removes a large amount of bubble in solution before occurring, and causes inside gel there are a large amount of stomata, gel it is equal
Even property is very poor, easily occurs in swelling process broken；(3) many drugs all have amino, if implement it is in situ carry medicine, easily with penta
Dialdehyde reacts；(4) glutaraldehyde is toxic, and residual is harmful to the human body.
In order to eliminate using chemical cross-linking agent and many drawbacks of bring, the metal for not needing chemical cross-linking agent is coordinated
The research of hydrogel has caused the very big concern of people.Coordination can make to form coordinate bond between macromolecule and metal ion
And obtain hydrogel, this crosslinked action can rapidly and efficiently crosslink polymer molecules, and be formed by hydrogel tool
There is self-repairability.Recently, mussel biomimetic chemistry causes the great interest of people, i.e., introduces catechu phenolic group in the side chain of polymer
It rolls into a ball to obtain to occur the derivative of metal coordination, solution and metal ion (Fe3+、Ca2+、Ag+) coordination formed
Hydrogel, but this hydrogel does not have light sensitivity, as bio-adhesive material, organizational project and controllable in drug release carrier
Aspect application has significant limitation.
Summary of the invention
Goal of the invention: aiming at the problems existing in the prior art, the first object of the present invention is to provide a kind of selfreparing, light
Sensitive aquagel, the hydrogel are free of any crosslinking agent, are provided simultaneously with self-repairability and light sensitivity, can be in ultraviolet lighting
It penetrates down and is changed into the hydrosol；The second object of the present invention is to provide the preparation method of the aquagel.
Technical solution: selfreparing of the present invention, photaesthesia aquagel, molecular structural formula are as follows:
A kind of selfreparing of the present invention, photaesthesia aquagel preparation method, include the following steps:
(1) acylation reaction is carried out using carboxymethyl chitosan and nitro dopamine as raw material, obtains the amine groups of DOPA containing nitro
Chitosan derivatives；
(2) by the aqueous solution and Fe of the chitosan derivatives of the amine groups of DOPA containing nitro3+Solution mixing, adjusts mixed solution
PH to 6.5~9.5, obtain selfreparing, photaesthesia aquagel.
Specifically, carboxymethyl chitosan is dissolved in the water in step (1), it is more that nitro is added under inert gas protection
Bar amine carries out acylation reaction, and dialysis, vacuum freeze drying, the chitosan for obtaining the amine groups of DOPA containing nitro spread out in water after reaction
Biology.
Preferably, the ratio between molal quantity of the quality of the carboxymethyl chitosan of addition and nitro dopamine is 100:0.5~5.
The molecular weight M of above-mentioned carboxymethyl chitosannFor 10kDa~100kDa, degree of substitution DsIt is 0.3~0.5.
Preferably, combination catalyst is added in acylation reaction, which is N- hydroxysuccinimide and 1- second
Base-(3- dimethylamino-propyl) carbodiimide.
Further, N- hydroxysuccinimide, 1- ethyl-(3- dimethylamino-propyl) carbodiimide and nitro dopamine
Molar ratio be 0.5~5:0.5~5:0.5~5.
Above-mentioned acylation reaction carries out at room temperature, and the reaction time is 5~50h.
In above-mentioned steps (2), the concentration of the chitosan derivatives aqueous solution of the amine groups of DOPA containing nitro is 10~80mg/
ML, Fe3+Fe in solution3+Concentration be 5~150mmol/L.
Further, Fe3+Solution is Fe3+Acid solution, when reaction, make sol pH variation slowly, gel can be prevented
It is too fast, influence its mechanical performance.
Inventive principle: firstly, amidation process occurs by the carboxyl of carboxymethyl chitosan and the amino of nitro dopamine
The nitro DOPA amine groups with fine metal coordination ability and photaesthesia are introduced, reaction process such as following formula:
Then the chitosan derivatives of obtained DOPA containing nitro amine groups are crosslinked with metallic ion coordination, makes to make
The aquagel obtained realizes self-repairability, meanwhile, dopamine has fine biocompatibility, so as to reduce hydrogel pair
The toxicity of organism, finally obtained aquagel have bioadhesive, self-repairability and light sensitivity.
The utility model has the advantages that compared with the prior art, the advantages of the present invention are as follows: (1) selfreparing of the invention, photaesthesia shell are poly-
Syrup gel has the characteristics that adhesion is strong, compliance is good, space-time controllability is strong, can be in wound face as wound dressing
On maintain a best moist environment for a long time, promote cell migration, so as to promote the epithelialization of wound, accelerate wound
The healing of mouth；(2) selfreparing of the invention, photaesthesia aquagel not only have self-repairability, also have light sensitivity,
Under wavelength 365nm ultraviolet light, which can be changed into the hydrosol, as wound care dressings, organizational project
Binder, drug controlled release carrier material have broad application prospects；(3) present invention change over by be chemically crosslinked come
The method for preparing hydrogel, and the method for using mussel biomimetic chemistry, dopamine have fine biocompatibility, by the way that nitre will be contained
The chitosan derivatives of Quito bar amine groups are crosslinked with metallic ion coordination, reduce the hydrogel pair of conventional method preparation
The toxicity of organism.
Detailed description of the invention
Fig. 1 is the molecular structural formula of selfreparing of the invention, photaesthesia aquagel；
Fig. 2 is selfreparing of the invention, the metamorphosis figure of photaesthesia aquagel during the preparation process；
Fig. 3 is the metamorphosis figure of selfreparing of the invention, photaesthesia aquagel in self-repair procedure；
Fig. 4 is the metamorphosis figure of selfreparing of the invention, photaesthesia aquagel in the case where UV illumination is penetrated.
Specific embodiment
Technical solution of the present invention is described further with reference to the accompanying drawing.
Selfreparing of the invention, photaesthesia aquagel, molecular structural formula such as Fig. 1.
The selfreparing, photaesthesia aquagel preparation method include the following steps:
(1) using carboxymethyl chitosan and nitro dopamine as raw material, pass through the carboxyl and nitro DOPA of carboxymethyl chitosan
It is that chitosan introduces the nitro DOPA amido with fine metal coordination ability and photaesthesia that amidation process, which occurs, for the amino of amine
Group, obtains the chitosan derivatives of the amine groups of DOPA containing nitro；
(2) by the aqueous solution of the chitosan derivatives of the certain density amine groups of DOPA containing nitro and certain density Fe3+
Solution mixing, Fe3+Monodentate, bidentate and three tooth complexs, while Fe can be formed with dopamine3+Oxidation is also had both, so as to
Obtain the aquagel with self-repairability and bioadhesive, such as Fig. 2.
Chitosan is native biopolymer, and source is extremely wide, safe and non-toxic, cheap, with synthesis macromolecule phase
Than, there is good biocompatibility and biodegradability, therefore, using chitosan as raw material, aquagel obtained
There is very high environment friendly and biological safety as wound dressing.
Embodiment 1
(1) chitosan derivatives of the amine groups of DOPA containing nitro are prepared
Take 100mg carboxymethyl chitosan (Mn=1.0 × 104Da, DS=0.3) it is dissolved in the deionized water of 50mL, logical
5mL hydroxysuccinimide Han 58mg (0.5mmol) N- (NHS) and 99mg (0.5mmol) nitro DOPA is added under nitrogen protection
It is sub- to add 96mg (0.5mmol) 1- ethyl-(3- dimethylamino-propyl) carbon two for the DMF solution of amine disulfate (Mn=198)
Amine (EDC), is stirred to react for 24 hours under room temperature.Two days, vacuum freeze drying of dialysis in deionized water obtain more containing nitro
The chitosan derivatives of bar amine groups.
(2) selfreparing, photaesthesia aquagel are prepared
The chitosan derivatives of the amine groups of DOPA containing nitro of step (1) preparation are dissolved in 20mL water and obtain concentration and are
The solution of 10mg/mL, with 5mL5mmol/L FeCl3Acetum is mixed, and the NaOH solution that 1mol/L is slowly added dropwise is adjusted
The pH to 6.5 of solution, obtains filemot aquagel.
The performance of aquagel made from the present embodiment is detected, specific experiment method and test result is as follows
One is tested to experiment four.
Experiment one: the self-repairability experiment of aquagel
Two parts of aquagels are made using step (1)~(2) method, then are close to place by them, it can by Fig. 3
To find out, after five minutes find two gels connect together, it is seen then that aquagel produced by the present invention have it is good from
Prosthetic.
Experiment two: the photosensitivity experiment of aquagel
By aquagel made from step (2) with wavelength be 365nm, the UV illumination that power is 20W is penetrated, and has figure
4 as can be seen that irradiate after ten minutes, which becomes the hydrosol.
Experiment three: the water absorption rate experiment of aquagel
The aquagel being completely dried is cut into diamond type sample and is weighed.Then, sample is immersed in phosphoric acid
Salt buffer (pH value 7.4；PBS), in specific time interval, i.e., sample is taken out into solution rapidly after 12 hours.With
After paper handkerchief wipes sample, its weight is weighed, until stopping measurement after sample water suction saturation.The water absorption rate of aquagel is pressed
Following formula are calculated:
Water absorption rate (%)=(Ws-Wd) × 100/Wd；
In formula, Wd represents weight when hydrogel drying, and Ws represents weight when hydrogel water suction is saturated.
The water absorption rate that experiment measures aquagel made from the present embodiment is 102%.
Experiment four: the adhesion strength experiment of aquagel
The fresh back normal skin tissue of pig is taken to cut into strips (4.0cm × 2.0cm, thick 1.5mm), by two pieces of skins
Organize 2.0cm closely smooth on one side；The chitosan derivatives of the amine groups of DOPA containing nitro made from step (1) are dissolved to obtain
Its aqueous solution (10mg/mL) takes 10 μ L chitosan aqueous solutions and 2 μ L Fe3+Solution mixing instills the adjacent sky of two skin histologies
Gap, then the NaOH solution of 1mol/L is slowly instilled, it can be observed that gap gradually forms filemot aquagel.5
After minute, its adhesion strength is tested with mechanics tester.
The adhesion strength that experiment measures the aquagel of gap formation is 135.8kPa.
Embodiment 2
(1) chitosan derivatives of the amine groups of DOPA containing nitro are prepared
Take 100mg carboxymethyl chitosan (Mn=3.6 × 104Da, DS=0.4) it is dissolved in the deionized water of 50mL, logical
It is more that 5mL hydroxysuccinimide Han 230mg (2.0mmol) N- (NHS) and 792mg (4.0mmol) nitro is added under nitrogen protection
The DMF solution of bar amine disulfate (Mn=198), adds 384mg (2.0mmol) 1- ethyl-(3- dimethylamino-propyl) carbon
Diimine (EDC), is stirred to react 5h under room temperature.Then in deionized water dialyse two days, vacuum freeze drying, contained
The chitosan derivatives of nitro DOPA amine groups.
(2) selfreparing, the preparation of photaesthesia chitosan gel rubber
The chitosan derivatives of the amine groups of DOPA containing nitro of step (1) preparation are dissolved in 20mL water and obtain concentration and are
40mg/mL solution, the FeCl with 5mL80mmol/L3Acetum is mixed, and the NaOH solution that 1mol/L is slowly added dropwise is adjusted
The pH of solution is 7.5, obtains filemot aquagel.
Experiment one: the self-repairability experiment of aquagel
Two parts of aquagels are made using step (1)~(2) method, then are close to place by them, after 7 minutes
It was found that two gels connect together.
Experiment two: the photosensitivity experiment of aquagel
By aquagel made from step (2) with wavelength be 365nm, the UV illumination that power is 20W is penetrated, irradiation
After 8 minutes, which becomes the hydrosol.
Experiment three: the water absorption rate experiment of aquagel
Referring to the water absorption rate test method in embodiment 1, the water absorption rate of aquagel made from the present embodiment is measured
It is 96.8%.
Experiment four: the adhesion strength experiment of aquagel
Referring to the adhesion strength testing method of embodiment 1, with the amido of DOPA containing nitro made from step in the present embodiment (1)
The chitosan derivatives of group form aquagel close to gap in two skin histologies for raw material, and the shell for measuring formation is poly-
The adhesion strength of syrup gel, testing the adhesion strength measured is 141.5kPa.
Embodiment 3
(1) chitosan derivatives of the amine groups of DOPA containing nitro are prepared
Take 100mg carboxymethyl chitosan (Mn=5 × 104Da, DS=0.4) it is dissolved in the deionized water of 50mL, in logical nitrogen
10mL hydroxysuccinimide Han 345mg (3.0mmol) N- (NHS) and 990mg (5.0mmol) nitro DOPA is added under gas shielded
The DMF solution of amine disulfate adds 576mg (3.0mmol) 1- ethyl-(3- dimethylamino-propyl) carbodiimide (EDC),
It is stirred to react 50h under room temperature.Then in deionized water dialyse two days, vacuum freeze drying, obtain dopamine containing nitro
The chitosan derivatives of group.
(2) selfreparing, the preparation of photaesthesia chitosan gel rubber
The chitosan derivatives of the amine groups of DOPA containing nitro of step (1) preparation are dissolved in 20mL water and obtain concentration and are
80mg/mL solution, the FeCl with 5mL100mmol/L3Acetum is mixed, and the NaOH solution tune of 1mol/L is slowly added dropwise
The pH for saving solution is 8.5, obtains filemot aquagel.
Self-repairability experiment and photosensitivity experiment, experimental result and embodiment 1 are carried out to aquagel obtained
Close, the aquagel obtained has good self-repairability and photosensitivity.
Referring to the water absorption rate test method and adhesion strength testing method in embodiment 1, shell made from the present embodiment is measured
The water absorption rate of glycan hydrogel is 113.7%, adhesion strength 121.4kPa.
Embodiment 4
(1) chitosan derivatives of the amine groups of DOPA containing nitro are prepared
Take 100mg carboxymethyl chitosan (Mn=10 × 104Da, DS=0.5) it is dissolved in the deionized water of 50mL, logical
5mL hydroxysuccinimide Han 575mg (5mmol) N- (NHS) and 990mg (5mmol) nitro dopamine is added under nitrogen protection
The DMF solution of disulfate (Mn=198) adds 910mg (5mmol) 1- ethyl-(3- dimethylamino-propyl) carbodiimide
(EDC), it is stirred to react 50h under room temperature.Then it dialyses two days, vacuum freeze drying, is obtained containing nitro in deionized water
The chitosan derivatives of DOPA amine groups.
(2) selfreparing, the preparation of photaesthesia chitosan gel rubber
The chitosan derivatives of the amine groups of DOPA containing nitro of step (1) preparation are dissolved in 25mL water and obtain concentration and are
80mg/mL solution, the FeCl with 5mL150mmol/L3Acetum is mixed, and the NaOH solution tune of 1mol/L is slowly added dropwise
The pH for saving solution is 9.5, obtains filemot aquagel.
Self-repairability experiment and photosensitivity experiment, experimental result and embodiment 1 are carried out to aquagel obtained
Close, the aquagel obtained has good self-repairability and photosensitivity.
Referring to the water absorption rate test method and adhesion strength testing method in embodiment 1, shell made from the present embodiment is measured
The water absorption rate of glycan hydrogel is 116.3%, adhesion strength 137.5kPa.
Influence of the amount of 5 nitro dopamine of embodiment to aquagel performance obtained
Referring to the method for embodiment 1,5 groups of parallel laboratory tests are carried out, difference is the nitro dopamine being added in step (1)
Amount is respectively 0.1mmol, 0.5mmol, 3mmol, 5mmol and 7mmol, the performance such as following table of final aquagel obtained
1。
The performance of aquagel made from different amounts of nitro dopamine is added in table 1
As can be seen from Table 1, the amount for the nitro dopamine being added in acylation reaction directly affects the chitosan water-setting to be formed
Self-healing properties, water imbibition and the adhesiveness of glue, when the quality of the carboxymethyl chitosan of addition and the molal quantity of nitro dopamine
The ratio between be 100:0.5~5 when, the selfreparing gelation time of aquagel is obviously shortened, only 5~6min, meanwhile,
Water imbibition and adhesiveness are obviously improved, and water absorption rate reaches 102%~107%, and adhesion strength reaches 135.8~141kpa, can be with
It is preferably attached on the surface of a wound, and maintains a best moist environment for a long time on wound face, accelerate the healing of wound.
Influence of the degree of substitution of 6 carboxymethyl chitosan of embodiment to aquagel performance obtained
Referring to the method for embodiment 1,5 groups of parallel laboratory tests are carried out, difference is the substitution of carboxymethyl chitosan in step (1)
Degree is respectively 0.2,0.3,0.4,0.5 and 0.6, performance such as the following table 2 of final aquagel obtained.
Table 2 is using the carboxymethyl chitosan of different degree of substitution as the performance of aquagel made from raw material
As shown in Table 2, the degree of substitution of carboxymethyl chitosan is also an important factor for influencing aquagel performance, to be elected to
When the degree of substitution of the carboxymethyl chitosan taken is 0.3~0.5, the self-repairability of the aquagel of formation, water imbibition and viscous
Attached property reaches most strong, and selfreparing gelation time is only 5~6min, meanwhile, water absorption rate is 102%~112%, is sticked
Intensity is 135.8~142kpa, is had excellent performance as wound care dressings, organizational project binder etc.；When carboxymethyl shell
The degree of substitution of glycan is greater than 0.5 or when less than 0.3, and the self-repairability of the aquagel of formation, water imbibition and adhesiveness are equal
Substantially weaken.
Claims (8)
1. a kind of selfreparing, photaesthesia aquagel, which is characterized in that the molecular formula of the aquagel is
The preparation method of the aquagel includes the following steps:
(1) acylation reaction is carried out using carboxymethyl chitosan and nitro dopamine as raw material, obtains the shell of the amine groups of DOPA containing nitro
Polysaccharid derivative；The molecular weight M of the carboxymethyl chitosannFor 10kDa~100kDa, degree of substitution DsIt is 0.3~0.5；
(2) by the aqueous solution and Fe of the chitosan derivatives of the amine groups of DOPA containing nitro3+Solution mixing, adjusts the pH of mixed solution
To 6.5~9.5, selfreparing, photaesthesia aquagel are obtained.
2. selfreparing according to claim 1, photaesthesia aquagel, which is characterized in that in step (1), by carboxylic
Methyl chitosan is dissolved in the water, and nitro dopamine is added under inert gas protection and carries out acylation reaction, after reaction in water
Dialysis, vacuum freeze drying, obtain the chitosan derivatives of the amine groups of DOPA containing nitro.
3. selfreparing according to claim 1 or 2, photaesthesia aquagel, which is characterized in that the carboxymethyl shell
The ratio between the quality of glycan and the molal quantity of nitro dopamine are 100:0.5~5.
4. selfreparing according to claim 1 or 2, photaesthesia aquagel, which is characterized in that the acylation reaction
Middle addition combination catalyst, the combination catalyst are N- hydroxysuccinimide and 1- ethyl-(3- dimethylamino-propyl) carbon two
Imines.
5. selfreparing according to claim 4, photaesthesia aquagel, which is characterized in that the N- hydroxyl fourth two
The molar ratio of acid imide, 1- ethyl-(3- dimethylamino-propyl) carbodiimide and nitro dopamine is 0.5~5:0.5~5:0.5
~5.
6. selfreparing according to claim 1, photaesthesia aquagel, which is characterized in that the acylation reaction exists
It carries out under room temperature, the reaction time is 5~50h.
7. selfreparing according to claim 1, photaesthesia aquagel, which is characterized in that described in step (2)
The concentration of the chitosan derivatives aqueous solution of the amine groups of DOPA containing nitro is 10~80mg/mL, Fe3+Fe in solution3+Concentration be
5~150mmol/L.
8. selfreparing according to claim 1, photaesthesia aquagel, which is characterized in that the Fe3+Solution is
Fe3+Acid solution.
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| CN110343194B (en) * | 2018-04-03 | 2021-07-16 | 中国科学院苏州纳米技术与纳米仿生研究所 | Chitosan sulfhydrylation derivative and preparation method and application thereof |
| CN109054051B (en) * | 2018-06-25 | 2020-08-28 | 浙江大学 | High-toughness self-repairing physical hydrogel based on dual ion coordination and preparation method thereof |
| CN109758675A (en) * | 2019-01-29 | 2019-05-17 | 青岛中腾生物技术有限公司 | A kind of self-healing medical gel |
| US20220347665A1 (en) * | 2019-06-19 | 2022-11-03 | Mcmaster University | Photoactive product comprising a catechol-containing compound and a photoelectrode comprising same |
| CN111588900B (en) * | 2020-05-21 | 2021-06-18 | 东华大学 | Waterproof breathable high-elastic self-repairing double-layer nanofiber membrane for skin dressing and preparation method thereof |
| CN113185714B (en) * | 2021-04-16 | 2022-08-09 | 江南大学 | High-adhesion antibacterial healing-promoting hydrogel and preparation method thereof |
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