CN106749628A - A kind of collagen polypeptide solution defecation method - Google Patents
A kind of collagen polypeptide solution defecation method Download PDFInfo
- Publication number
- CN106749628A CN106749628A CN201611034468.8A CN201611034468A CN106749628A CN 106749628 A CN106749628 A CN 106749628A CN 201611034468 A CN201611034468 A CN 201611034468A CN 106749628 A CN106749628 A CN 106749628A
- Authority
- CN
- China
- Prior art keywords
- collagen polypeptide
- solution
- shitosan
- polypeptide solution
- defecation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 28
- 108010035532 Collagen Proteins 0.000 title claims abstract description 28
- 229920001436 collagen Polymers 0.000 title claims abstract description 28
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 24
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000013872 defecation Effects 0.000 title claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 12
- 229920001661 Chitosan Polymers 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 230000000850 deacetylating effect Effects 0.000 claims 1
- 230000003301 hydrolyzing effect Effects 0.000 claims 1
- 238000005352 clarification Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 10
- 238000005189 flocculation Methods 0.000 description 9
- 230000016615 flocculation Effects 0.000 description 9
- 238000001914 filtration Methods 0.000 description 8
- 230000006196 deacetylation Effects 0.000 description 4
- 238000003381 deacetylation reaction Methods 0.000 description 4
- 239000003643 water by type Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000003311 flocculating effect Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention discloses a kind of collagen polypeptide solution defecation method, using shitosan as flocculant, for the solution clarification in collagen polypeptide production process, step is simple, it is easy to operate.
Description
Technical field
The invention belongs to technical field of biochemical industry, more particularly, to a kind of collagen polypeptide solution defecation method.
Background technology
Collagen (collagen) accounts for the 30~40% of people's vivo protein total amount, is the most albumen of in-vivo content
Matter, amino acid composition is special, and glycine (Gly) accounts for 30%, and proline (Pro) and hydroxyproline (Hyp) account for about 25% altogether.
The solution that protease hydrolyzed method extracts obtained by collagen polypeptide from animal tissue typically contains many insoluble matters,
Feed liquid is caused not clarified, these insoluble particles will not quickly be assembled or settle.Filtering is that collagen polypeptide conventional at present is clear
Clearing method, but the generally effect on driving birds is not good of clarification, filtration time are more long, and feed liquid is apt to deteriorate.Adding flocculant can effectively promote
Feeding liquid is clarified and is conducive to shortening filtration time.
From shitosan as the existing document report of flocculant.Chinese patent " the flocculation clarification side of a Plant Extracts
Mention in method (application number 201510902137.0) " being shared using shitosan and polyacrylamide and realize the clear of Chinese medical extract
Clearly.It is usually to remove the insoluble protein in solution as flocculant to use shitosan at present, uses shitosan in the present invention
In the clarification of collagen solution, remove insoluble matter, the method for retaining protein is there is not yet document report.
The content of the invention
It is clear a kind of effective collagen polypeptide solution to be provided it is an object of the invention to improve the deficiency of prior art
Clearing method.The defecation method of feed liquid is different types of filtering in existing collagen polypeptide production technology.After protease hydrolyzed
Collagen polypeptide solution typically contain many insoluble matters, the direct rate of filtration is slower.If it is therein to add flocculant to make
Insoluble component rapid flocculation is settled, then will improve the rate of filtration and filter quality.Selection shitosan as flocculant, be due to
It comes from natural products, and operating procedure is simple, no toxic and side effect, reach and can remove completely after flocculating effect, do not influence
End product quality, the flocculation sediment for being formed can be kept for a very long time, even if mild agitation will not also be broken up.
The object of the present invention is achieved like this, the collagen polypeptide solution after protease hydrolyzed, adds shitosan to make
Be flocculant, stirring to insoluble matter flocculation it is agglomerating after, stand appropriate time after filter clarification after.
The addition of shitosan is the important influence factor of clarification.The addition weight of shitosan is that collagen polypeptide is molten
The 0.01~0.05% of liquid weight.It is directed to for collagen polypeptide solution, the addition less than 0.01% is typically not enough to
All insoluble matters are precipitated, the addition more than 0.05%, it is possible to the waste of shitosan can be caused, and it is possible to anti-
And have slight adverse effect to the final turbidity of feed liquid.
The shitosan of selection deacetylation (70~90%) higher holds because its quality, especially viscosity is more stable
Preferable flocculating effect is easily reached in different process.Shitosan need to dissolve in advance before.Because shitosan is dissolved in
Sour environment, therefore chitosan solution pH is adjusted using hydrochloric acid or acetic acid, stabilize it 2.5~3.0.This pH scope
Under, shitosan can fully dissolve, and will not decompose quickly causes the viscosity to reduce, so as to influence flocculating effect.Shitosan is matched somebody with somebody
Being made concentration of aqueous solution should be limited to 1~3%, and too low concentration is not easy to operate aborning, and too high concentration can increase shell and gather
The dissolution time of sugar, it is necessary to lower pH can just be completely dissolved when chitosan solution concentration increases.Shitosan is configured to the aqueous solution
After should use as early as possible, the resting period was no more than 12 hours.Ionic strength higher is unfavorable for the flocculation of shitosan, the material for the treatment of
Liquid should avoid hypersaline environment.Shitosan can be dissolved in acid solution, therefore collagen polypeptide solution should keep partial neutral or alkalescence
Environment.Feed temperature is too high also to influence the precipitation of flocculation, therefore feed temperature should to control below 40 DEG C.
Specific embodiment：
Embodiment 1, a kind of collagen polypeptide solution defecation method, by 100 kilograms of input retort of dry fish scale after decalcification
In, 1000 liters of purified waters are added, boil 30 minutes；55~65 DEG C are cooled the temperature to, pH 5.5 is controlled, 1 kilogram of neutral egg is added
White enzyme, digests 4 hours, and enzymolysis is warming up to 90 DEG C after terminating, and is incubated 30 minutes；Solution is cooled to 10 DEG C, now feed liquid turbidity
More than 200NTU.
Extemporaneous preparation chitosan solution, takes 100 grams of shitosans of deacetylation 70%, is placed in 10 liters of purified waters, uses salt
Acid maintains pH 3.0, and stirring dissolves shitosan.Chitosan solution is slowly added into collagen polypeptide hydrolyzate and kept
Stirring, stops stirring and standing after occurring visible flocculation after about 10 minutes, and supernatant is taken in different time of repose, fixed using middling speed
Property filter paper filter under gravity after detect turbidity such as following table.
| Time of repose/hour | 1 | 4 | 8 |
| Turbidity/NTU | 9.4 | 8.9 | 8.7 |
Embodiment 2, a kind of collagen polypeptide solution defecation method, enzyme solution is with embodiment 1, and gained feed liquid turbidity is big
In 200NTU, pH 6.5,20 DEG C of temperature are adjusted.
Extemporaneous preparation chitosan solution, takes 200 grams of shitosans of deacetylation 80%, is placed in 10 liters of purified waters, uses vinegar
Acid maintains pH 2.8, and stirring dissolves shitosan.Chitosan solution is slowly added into collagen polypeptide hydrolyzate and keeps stirring
Mix, stop stirring and standing after occurring visible flocculation after about 10 minutes, supernatant is taken in different time of repose, it is qualitative using middling speed
Filter paper detects turbidity such as following table after filtering under gravity.
| Time of repose/hour | 1 | 4 | 8 |
| Turbidity/NTU | 5.0 | 4.9 | 4.7 |
Embodiment 3, a kind of collagen polypeptide solution defecation method, enzyme solution is with embodiment 1, and gained feed liquid turbidity is big
In 200NTU, pH 7.5,40 DEG C of temperature are adjusted.
Extemporaneous preparation chitosan solution, takes 500 grams of shitosans of deacetylation 90%, is placed in 20 liters of purified waters, uses salt
Acid maintains pH 2.5, and stirring dissolves shitosan.Chitosan solution is slowly added into collagen polypeptide hydrolyzate and keeps stirring
Mix, stop stirring and standing after occurring visible flocculation after about 10 minutes, supernatant is taken in different time of repose, it is qualitative using middling speed
Filter paper detects turbidity such as following table after filtering under gravity.
| Time of repose/hour | 1 | 4 | 8 |
| Turbidity/NTU | 4.1 | 3.9 | 4.1 |
Claims (3)
1. a kind of collagen polypeptide solution defecation method, it is characterized in that in the protease hydrolytic liquid of collagen, adding molten
The shitosan of liquid weight 0.01~0.05% is flocculated, the insoluble matter in removal hydrolyzate.
2. a kind of collagen polypeptide solution defecation method according to claim 1, the deacetylating degree of chitosan for being used
70~90%, it is using hydrochloric acid or acetic acid that pH value of solution is steady using the preceding aqueous solution that shitosan is configured to concentration 1~3% in advance
It is scheduled on 2.5~3.0.
3. a kind of collagen polypeptide solution defecation method according to claim 1, before adding chitosan solution, need to first by
Collagen polypeptide solution adjusts pH5.0~8.0,10~40 DEG C of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611034468.8A CN106749628A (en) | 2016-11-15 | 2016-11-15 | A kind of collagen polypeptide solution defecation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611034468.8A CN106749628A (en) | 2016-11-15 | 2016-11-15 | A kind of collagen polypeptide solution defecation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106749628A true CN106749628A (en) | 2017-05-31 |
Family
ID=58971884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611034468.8A Pending CN106749628A (en) | 2016-11-15 | 2016-11-15 | A kind of collagen polypeptide solution defecation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106749628A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370446A (en) * | 2018-12-19 | 2019-02-22 | 潍坊学院 | A kind of method of waste residue comprehensive utilization clarification gelatin solution |
| CN112515033A (en) * | 2020-12-07 | 2021-03-19 | 武汉纺织大学 | Collagen active peptide for balancing hormone in vivo and production process thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329843A (en) * | 2011-08-05 | 2012-01-25 | 蚌埠丰原明胶有限公司 | Enzyme method for preparing gelatin |
| CN105331665A (en) * | 2015-12-09 | 2016-02-17 | 梁尚文 | Method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis |
| CN106086139A (en) * | 2016-08-10 | 2016-11-09 | 柳江县渡庄生物科技有限公司 | A kind of method utilizing fresh-water fishes noggin enzymolysis to prepare fish head polypeptides |
| CN106282283A (en) * | 2016-08-10 | 2017-01-04 | 柳江县渡庄生物科技有限公司 | A kind of microwave-assisted prepares the method for freshwater fish bones protein polypeptide |
-
2016
- 2016-11-15 CN CN201611034468.8A patent/CN106749628A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329843A (en) * | 2011-08-05 | 2012-01-25 | 蚌埠丰原明胶有限公司 | Enzyme method for preparing gelatin |
| CN105331665A (en) * | 2015-12-09 | 2016-02-17 | 梁尚文 | Method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis |
| CN106086139A (en) * | 2016-08-10 | 2016-11-09 | 柳江县渡庄生物科技有限公司 | A kind of method utilizing fresh-water fishes noggin enzymolysis to prepare fish head polypeptides |
| CN106282283A (en) * | 2016-08-10 | 2017-01-04 | 柳江县渡庄生物科技有限公司 | A kind of microwave-assisted prepares the method for freshwater fish bones protein polypeptide |
Non-Patent Citations (3)
| Title |
|---|
| RAN YANG，HAIJIANG LI，MU HUANG，HU YANG，AIMIN LI: "A review on chitosan-based flocculants and their applications in water treatment", 《WATER RESEARCH》 * |
| 姜燕,尤婷婷,柳佳齐,鲍慧娟.: "壳聚糖絮凝作用在中药和果酒澄清中的应用", 《食品工业》 * |
| 谢婷玉,荆肇乾,王郑,徐佳莹: "壳聚糖的絮凝性能及其在废水脱色处理中的应用", 《化工环保》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370446A (en) * | 2018-12-19 | 2019-02-22 | 潍坊学院 | A kind of method of waste residue comprehensive utilization clarification gelatin solution |
| CN112515033A (en) * | 2020-12-07 | 2021-03-19 | 武汉纺织大学 | Collagen active peptide for balancing hormone in vivo and production process thereof |
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Application publication date: 20170531 |