CN106749434A - A kind of Application way of FOS chromatogram raffinate - Google Patents
A kind of Application way of FOS chromatogram raffinate Download PDFInfo
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- CN106749434A CN106749434A CN201611047756.7A CN201611047756A CN106749434A CN 106749434 A CN106749434 A CN 106749434A CN 201611047756 A CN201611047756 A CN 201611047756A CN 106749434 A CN106749434 A CN 106749434A
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- Prior art keywords
- fos
- chromatogram
- nanofiltration
- raffinate
- application way
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0051—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Fructofuranans, e.g. beta-2,6-D-fructofuranan, i.e. levan; Derivatives thereof
Abstract
The present invention relates to a kind of Application way of FOS chromatogram raffinate, comprise the following steps：（1）By the content of monose in nanofiltration reduction FOS chromatogram raffinate, the content of wherein sucrose and FOS is improved；（2）The dispensing link that trapped fluid after nanofiltration is returned in set to FOS production.The utilization rate of sucrose can be improved by aforesaid operations, and then is increased economic efficiency.
Description
Technical field
The invention belongs to the technical field of oligosaccharide, it is related to a kind of Application way of FOS chromatogram raffinate.
Background technology
FOS（Fructooligosaccharides,FOS）Refer to that fructosyl is formed by connecting through β -2,1 glycosidic bonds
The degree of polymerization is 2 ~ 9 functional oligose, belongs to food ingredient.By structure, FOS can be divided into sugarcane-fruit type and fruit-fruit type
Two kinds.Industrial production FOS mainly has two methods：Enzyme hydrolysis method with inulin as raw material and with sucrose as raw material
Enzyme transforming process.The former gained FOS polymerization scope is larger（DP2-DP9）, belong to fruit-fruit type；The latter's gained FOS gathers
Right scope is smaller（DP3-DP6）, belong to sugarcane-fruit type.Research display, animal or human body are ingested FOS, can improve enterobacteriaceae
Group, mitigation constipation symptom, the absorption, the strengthen immunity that improve lipid-metabolism, suppression enteron aisle corruption, promote the mineral elements such as calcium and magnesium
Deng.FOS good physicochemical property and physiological function, make it obtained in the industries such as food, health products, feed extensively
Using.
It is industrial have microbial liquid tank fermentation method in the method that sucrose is raw material production FOS, dissociate enzyme process and
Immobilized enzyme method.The purity of which kind of method gained FOS is not high（No more than 62%）, remaining composition include 25-30%
Monose（Mainly glucose, also a small amount of fructose）With the sucrose of 10-15%.Contents of monosaccharides higher, limits FOS
Edible crowd.Such as, diabetes patient is with regard to inedibility.This requires to produce high-purity fructo oligosaccharides.General FOS
Purity is more than 90%, it is considered to be high-purity fructo oligosaccharides.
The production method of high-purity fructo oligosaccharides mainly has fermentation method, nano filtering process and chromatography.Fermentation method is conventional early stage
The method for preparing high-purity fructo oligosaccharides, the shortcoming of the method is that the accessory substance that fermentation is produced is difficult to remove；Nano filtering process is a kind of
Physical separation method, in theory without loss of material, but the method water consumption is big, and feed concentration is relatively low after nanofiltration, later stage concentration
It is relatively costly；Chromatography is the method widely used during current high-purity fructo oligosaccharides are produced.FOS liquid is through chromatogram point
Extract solution can be produced after（BD liquid）And raffinate（AD liquid）, BD liquid is the feed liquid containing the FOS of purity more than 95%, AD liquid
It is glucose content feed liquid higher.AD liquid it is general it is concentrated after sell.
The A of patent CN 104878056 disclose a kind of method for producing high-purity fructo oligosaccharides, using the color of continuous automatic control
Spectrum technique is separated, reclaim after a chromatographic isolation containing FOS, sucrose, glucose extract solution, by two dimensional chromatography point
Separate out sucrose therein and FOS, then make sugar composition generation FOS therein by enzymatic conversion, and by this thing
Material is recycled as a raw material for chromatogram, so as to reduce the raw material consumption of high-purity fructo oligosaccharides.Once carried described in the patent
Take liquid（AD liquid i.e. in this patent）, secondary retained liquid（BD liquid after two dimensional chromatography）It is both needed to further locate by concentration
Reason, and gained trapped fluid can directly return set to saccharification link after this patent nanofiltration, without concentration, cost is relatively low.
AD liquid sells that profit is relatively low, the invention provides a kind of Application way of AD liquid, to improve raw material sucrose
Utilization rate, and then increase economic efficiency.
The content of the invention
The invention provides a kind of Application way of FOS chromatogram raffinate.
A kind of Application way of FOS chromatogram raffinate, comprises the following steps：
（1）By the content of monose in nanofiltration reduction FOS chromatogram raffinate, improve wherein sucrose and FOS and contain
Amount.The molecular cut off scope of NF membrane is 150 ~ 600D；The running temperature of nanofiltration be 35 ~ 55 DEG C, operating pressure be 20 ~
40bar；It is dense that feed liquid is concentrated into sugar（Brix）25 ~ 35% start to add water, and control in the concentration range, and the total amount of water of nanofiltration is
3 ~ 5 times of feed volume.
（2）The dispensing link that nanofiltration gained trapped fluid is used in FOS production, that is, return set.Return dry in set feed liquid
Base is 1 with the mass ratio of raw material sucrose:4~10.
Beneficial effect
The content for improving sucrose and FOS in FOS chromatogram raffinate is processed by nanofiltration, and returns set to dispensing ring
Section, can put forward the utilization rate of sucrose, and then increase economic efficiency.
Specific embodiment
Technical scheme is described further with reference to embodiment, but institute's protection domain of the present invention is not limited to
This.
Embodiment 1
（1）Nanofiltration treatment is carried out to FOS chromatogram raffinate：The molecular cut off scope of NF membrane is 150 ~ 300D；Nanofiltration
40 ~ 45 DEG C of running temperature, 30 ~ 35bar of operating pressure；It is dense that feed liquid is concentrated into sugar（Brix）25 ~ 28% start to add water, and control
In the concentration range, the total amount of water of nanofiltration is 5 times of feed volume.
（2）The dispensing link that above-mentioned nanofiltration gained trapped fluid is returned in set to FOS production, returns dry in set feed liquid
The ratio of base and raw material sucrose is 1:4.
Embodiment 2
（1）Nanofiltration treatment is carried out to FOS chromatogram raffinate：The molecular cut off scope of NF membrane is 300 ~ 500D；Nanofiltration
45 ~ 50 DEG C of running temperature, 25 ~ 30bar of operating pressure；It is dense that feed liquid is concentrated into sugar（Brix）28 ~ 31% start to add water, and control
In the concentration range, the total amount of water of nanofiltration is 4 times of feed volume.
（2）The dispensing link that above-mentioned nanofiltration gained trapped fluid is returned in set to FOS production, returns dry in set feed liquid
The ratio of base and raw material sucrose is 1:6.
Embodiment 3
（1）Nanofiltration treatment is carried out to FOS chromatogram raffinate：The molecular cut off scope of NF membrane is 400 ~ 600D；Nanofiltration
35 ~ 40 DEG C of running temperature, 35 ~ 40bar of operating pressure；It is dense that feed liquid is concentrated into sugar（Brix）31 ~ 35% start to add water, and control
In the concentration range, the total amount of water of nanofiltration is 3 times of feed volume.
（2）The dispensing link that above-mentioned nanofiltration gained trapped fluid is returned in set to FOS production, returns dry in set feed liquid
The ratio of base and raw material sucrose is 1:8.
Embodiment 4
（1）Nanofiltration treatment is carried out to FOS chromatogram raffinate：The molecular cut off scope of NF membrane is 300 ~ 500D；Nanofiltration
50 ~ 55 DEG C of running temperature, 30 ~ 35bar of operating pressure；It is dense that feed liquid is concentrated into sugar（Brix）29 ~ 32% start to add water, and control
In the concentration range, the total amount of water of nanofiltration is 4 times of feed volume.
（2）The dispensing link that above-mentioned nanofiltration gained trapped fluid is returned in set to FOS production, returns dry in set feed liquid
The ratio of base and raw material sucrose is 1:10.
Embodiment 5
（1）Nanofiltration treatment is carried out to FOS chromatogram raffinate：The molecular cut off scope of NF membrane is 150 ~ 300D；Nanofiltration
45 ~ 50 DEG C of running temperature, 32 ~ 37bar of operating pressure；It is dense that feed liquid is concentrated into sugar（Brix）26 ~ 29% start to add water, and control
In the concentration range, the total amount of water of nanofiltration is 5 times of feed volume.
（2）The dispensing link that above-mentioned nanofiltration gained trapped fluid is returned in set to FOS production, returns dry in set feed liquid
The ratio of base and raw material sucrose is 1:5.
Above-described embodiment is not limited for the present invention preferably implementation method, but embodiments of the present invention by embodiment
System, other it is any without departing from Spirit Essence of the invention and the change, modification made under principle, combine, substitute, simplify and should be
Equivalence replacement mode, is included within protection scope of the present invention.
Claims (5)
1. a kind of Application way of FOS chromatogram raffinate, it is characterised in that comprise the following steps：
（1）By the content of monose in nanofiltration reduction FOS chromatogram raffinate, improve wherein sucrose and FOS and contain
Amount；
（2）The dispensing link that trapped fluid after nanofiltration is returned in set to FOS production, sells after permeate is concentrated.
2. the Application way of FOS chromatogram raffinate according to claim 1, it is characterised in that the step（1）
In, the molecular cut off scope of NF membrane used is 150 ~ 600D.
3. the Application way of FOS chromatogram raffinate according to claim 1, it is characterised in that the step（1）
In, nanofiltration running temperature is 35 ~ 55 DEG C, and operating pressure is 20 ~ 40bar.
4. the Application way of FOS chromatogram raffinate according to claim 1, it is characterised in that the step（1）
In, it is dense that feed liquid is concentrated into sugar（Brix）25 ~ 35% start to add water, and control in the concentration range, and the total amount of water of nanofiltration is charging
3 ~ 5 times of volume.
5. the Application way of FOS chromatogram raffinate according to claim 1, it is characterised in that the step（2）
In, when the trapped fluid after nanofiltration returns set, the butt returned in set feed liquid is 1 with the mass ratio of raw material sucrose:4~10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611047756.7A CN106749434A (en) | 2016-11-25 | 2016-11-25 | A kind of Application way of FOS chromatogram raffinate |
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| CN201611047756.7A CN106749434A (en) | 2016-11-25 | 2016-11-25 | A kind of Application way of FOS chromatogram raffinate |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376901A (en) * | 2008-09-27 | 2009-03-04 | 江南大学 | Preparation of high-purity fructo oligosaccharides |
| CN101691538A (en) * | 2009-09-29 | 2010-04-07 | 保龄宝生物股份有限公司 | Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same |
| CN103980382A (en) * | 2014-05-28 | 2014-08-13 | 南京工业大学 | Method for purifying fructose oligosaccharide |
| CN104531809A (en) * | 2014-12-04 | 2015-04-22 | 青海威德生物技术有限公司 | Preparation and purification method of fructo-oligosaccharide |
| CN104878056A (en) * | 2015-04-27 | 2015-09-02 | 量子高科(中国)生物股份有限公司 | Method for producing high-purity fructo-oligose |
| CN105801717A (en) * | 2016-03-30 | 2016-07-27 | 青海威德生物技术有限公司 | Method for producing fructo-oligosaccharide through membrane separation method |
| CN105950684A (en) * | 2016-07-01 | 2016-09-21 | 白银熙瑞生物工程有限公司 | Method for producing fructooligosaccharides by using jerusalem artichoke as raw material |
-
2016
- 2016-11-25 CN CN201611047756.7A patent/CN106749434A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376901A (en) * | 2008-09-27 | 2009-03-04 | 江南大学 | Preparation of high-purity fructo oligosaccharides |
| CN101691538A (en) * | 2009-09-29 | 2010-04-07 | 保龄宝生物股份有限公司 | Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same |
| CN103980382A (en) * | 2014-05-28 | 2014-08-13 | 南京工业大学 | Method for purifying fructose oligosaccharide |
| CN104531809A (en) * | 2014-12-04 | 2015-04-22 | 青海威德生物技术有限公司 | Preparation and purification method of fructo-oligosaccharide |
| CN104878056A (en) * | 2015-04-27 | 2015-09-02 | 量子高科(中国)生物股份有限公司 | Method for producing high-purity fructo-oligose |
| CN105801717A (en) * | 2016-03-30 | 2016-07-27 | 青海威德生物技术有限公司 | Method for producing fructo-oligosaccharide through membrane separation method |
| CN105950684A (en) * | 2016-07-01 | 2016-09-21 | 白银熙瑞生物工程有限公司 | Method for producing fructooligosaccharides by using jerusalem artichoke as raw material |
Non-Patent Citations (2)
| Title |
|---|
| 冯文亮 等: "纳滤技术生产高纯度低聚果糖的研究", 《乳业科学与技术》 * |
| 武金良: "高纯低聚果糖分离纯化技术的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
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Application publication date: 20170531 |