CN106745822B - A method of active sludge microorganism suspend mode and loss are differentiated based on breathing map - Google Patents
A method of active sludge microorganism suspend mode and loss are differentiated based on breathing map Download PDFInfo
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- CN106745822B CN106745822B CN201710100609.XA CN201710100609A CN106745822B CN 106745822 B CN106745822 B CN 106745822B CN 201710100609 A CN201710100609 A CN 201710100609A CN 106745822 B CN106745822 B CN 106745822B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a kind of methods that active sludge microorganism suspend mode and loss are differentiated based on breathing map, comprising: 1) carries out breathing atlas analysis to the activated sludge of sewage treatment plant, measure autotrophic bacterium and heterotroph OUR value under field condition；2) standard is carried out to the activated sludge of reaction tank and breathes atlas analysis, measure autotrophic bacterium and heterotroph OUR value under standard conditions；3) if OUR reduces under field condition and OUR illustrates that autotrophic bacterium or heterotroph enter dormant state when significant change not occurring under standard conditions；If 4) OUR is obviously reduced under standard conditions, illustrate that autotrophic bacterium or heterotroph are lost.The present invention can breathe map according to activated sludge and differentiate active sludge microorganism suspend mode and attrition status.By the method, the technical work personnel of sewage plant can be made to understand the processing capacity of current active sludge, adjust operating parameter in time, provide guiding opinion with energy-saving for the reasonable operation of sewage treatment plant.
Description
Technical field
The invention belongs to sewage treatment fields, are related to a kind of by passing through activated sludge to activated sludge scene and standard
Condition breathes the analysis of map, determines the suspend mode of sewage plant activated sludge and is lost the method for situation.
Background technique
The main process of wastewater treatment in China factory sewage treatment at present mainly uses activated sludge process.Active sludge microorganism
Activity be directly related to the effect of sewage treatment, and temperature largely affects the activity of microorganism, to influence
The treatment effeciency of sewage treatment plant.When the temperature is low, part microbes enter dormant state and can not play sewage treatment
Effect, if temperature continues to reduce, microorganism can be lost, and seriously affect the sewage treatment capacity of activated sludge.China is cold
The operation of cryogenic region winter sewage treatment plant is still a problem.The suspend mode of active sludge microorganism and loss situation and sewage
The treatment effect for the treatment of plant is closely related, when active sludge microorganism in a dormant state when, only need appropriate adjustment operating parameter
Condition can make microorganism activity recovery；When active sludge microorganism is lost, then need to ensure out by increasing the modes such as sludge age
Water water quality reaching standard.But the suspend mode and loss of current microorganism still lack accurate judgment basis, so method is to sewage treatment plant
Operational management have guiding significance.
Summary of the invention
The purpose of the present invention is to provide a kind of sides that active sludge microorganism suspend mode and loss are differentiated based on breathing map
Method, this method are an activated sludge index using scene and microorganism oxygen consumption rate under standard conditions, pass through the scene of measurement
The situation of active sludge microorganism suspend mode and loss is determined with map is breathed under standard conditions, is taken enhancements in time, is
The overwintering water quality guarantee of sewage plant provides theoretical foundation.
The purpose of the present invention is what is be achieved through the following technical solutions.
What is provided according to embodiments of the present invention is a kind of based on the differentiation active sludge microorganism suspend mode of breathing map and loss
Method, which is characterized in that include the following steps:
1) sludge sewage is taken；
2) breathing map test under continuous 2~7 days field conditions is carried out to activated sludge, respectively obtained existing under field condition
Shape temperature OURs, quasi- endogenous oxygen consumption rate OURq, endogenous oxygen consumption rate OURePlus oxygen consumption rate OUR after nitrogen sourceenWith total consumption
Oxygen rate OURencMeasurement data；
3) it is calculated by measurement data
OURn=OURen-OURe,
OURc=OURenc-OURen；
Wherein, OURnFor autotrophic bacterium oxygen consumption rate under field condition；OURcFor heterotroph oxygen consumption rate under field condition；
4) breathing map test under continuous 2~7 days standard conditions is carried out to sewage plant activated sludge, respectively obtains standard bar
OUR ' under parts, quasi- endogenous oxygen consumption rate OUR 'q, endogenous oxygen consumption rate OUR 'ePlus oxygen consumption rate OUR ' after nitrogen sourceenWith total oxygen consumption
Rate OUR 'enc；
5) it is calculated by measurement data
OUR’n=OUR 'en-OUR’e,
OUR’c=OUR 'enc-OUR’en；
Wherein, OUR 'nFor autotrophic bacterium oxygen consumption rate under standard conditions；OUR'cFor heterotroph oxygen consumption rate under standard conditions；
6) in the continuous data of multiple groups:
As autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnStablize constant, and autotrophic bacterium oxygen consumption rate OUR under field conditionn
When reduction, then there are suspend mode situations for activated sludge autotrophic bacterium；If autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnWith live item
Autotrophic bacterium oxygen consumption rate OUR under partnDifference is bigger, then activated sludge autotrophic bacterium suspend mode amount is more；
As heterotroph oxygen consumption rate OUR ' under standard conditionscStablize constant, and heterotroph oxygen consumption rate OUR under field conditionc
When reduction, then there are suspend mode situations for activated sludge heterotroph；If heterotroph oxygen consumption rate OUR ' under standard conditionscWith live item
Heterotroph oxygen consumption rate OUR under partcDifference is bigger, then activated sludge heterotroph suspend mode amount is more；
7) in the continuous data of multiple groups:
As autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnWhen being obviously reduced, then activated sludge autotrophic bacterium starts to be lost, together
When standard conditions under autotrophic bacterium oxygen consumption rate OUR 'nWhat is reduced is more, and activated sludge autotrophy bacterium amount is lost more；
As heterotroph oxygen consumption rate OUR ' under standard conditionscWhen being obviously reduced, then activated sludge heterotroph starts to be lost, together
When standard conditions under heterotroph oxygen consumption rate OUR 'cWhat is reduced is more, and activated sludge heterotrophism bacterium amount is lost more.
Further, the detailed process of map test is breathed in step 2) under field condition are as follows:
In the case where temperature keeps with sewage plant reaction tank uniform condition, sludge sewage is taken, and with volume ratio 1:3
Ratio addition tap water dilution, measure field condition under status oxygen consumption rate OURs；By activated sludge sample by stirring, sinking
1/2 volume forming sediment, remove supernatant, being settled to activated sludge dilution, washes mud with PBS buffer solution, and the standard for measuring sludge is endogenous
Oxygen consumption rate OURq；Active sludge intermixture is settled to the original volume of activated sludge dilution by addition tap water later, is aerated,
Activated sludge enters endogenous respiration state, measures its endogenous oxygen consumption rate OURe；It is surveyed and is added than ammonium chloride is added by certain mass again
Oxygen consumption rate OUR after nitrogen sourceen=OURe+OURn；Enough anhydrous sodium acetates are eventually adding, guarantee that matrix is sufficient, measure total consumption
Oxygen rate OURenc=OURe+OURn+OURc。
Further, in step 3), the detailed process of map test is breathed under standard conditions are as follows:
Under the conditions of temperature is 20 DEG C, sludge sewage is taken, and add tap water with the ratio of volume ratio 1:3
Dilution measures status oxygen consumption rate OUR ' under standard conditionss；By activated sludge sample by stirring, precipitating, going supernatant, determine
Hold to 1/2 volume of activated sludge dilution, wash mud with PBS buffer solution, measures the endogenous oxygen consumption rate OUR ' of standard of sludgeq；
Active sludge intermixture is settled to the original volume of activated sludge dilution, aeration by addition tap water later, and activated sludge enters
Endogenous respiration state measures its endogenous oxygen consumption rate OUR 'e；Oxygen consumption after adding nitrogen source is surveyed than ammonium chloride is added by certain mass again
Rate OUR 'en=OUR 'e+OUR’n；Enough anhydrous sodium acetates are eventually adding, guarantees that matrix is sufficient, measures total oxygen consumption rate
OUR’enc=OUR 'e+OUR’n+OUR’c。
The buffer solution that this method uses is following components mixed liquor:
A:KH2PO4Concentration is 1.5~2.5mmolL-1；B:Na2HPO4Concentration is 8~12mmolL-1；C:NaCl concentration
For 135~140mmolL-1；D:KCl concentration is 2.5~3.0mmolL-1.It is washed mud 3~5 times with PBS buffer solution.
Further, active sludge intermixture is settled to the original volume of activated sludge dilution by addition tap water, and aeration 2~
4h。
Further, ammonium chloride is added according to 40~60mg/L.
Further, anhydrous sodium acetate is added according to 250~350mg/L.
The present invention can not differentiate active sludge microorganism suspend mode and loss situation thus can not for traditional wastewater treatment plant
The limitation for assessing the sewage treatment capacity of sewage plant provides one kind by the breathing map of activated sludge from sludge self-characteristic
Differentiate active sludge microorganism suspend mode and be lost the simple and easy method of situation, is the stable fortune of sewage treatment plant under the conditions of winter low temperature
Row provides theory significance with operation regulation.
Compared with the existing technology, present invention is characterized in that
1) the method for the present invention is fast and effective.Scene based on activated sludge determines activity with the breathing map under standard conditions
Sludge microbe suspend mode and loss situation, can effectively and rapidly determine the processing capacity of activated sludge, take reinforcing to arrange in time
It applies, ensures the stable operation of sewage treatment plant.
2) the method for the present invention is easy to detect.Step is simple and easy, test equipment automation.Such as use the green mark water ring in Xi'an
The WBM400 type sewage treatment wisdom that border Science and Technology Ltd. provides runs work station, can be automatic in the case where unattended
Change detects sludge to be detected.
Detailed description of the invention
The respectively scene Fig. 1 (a), Fig. 1 (b), Fig. 1 (c) is with autotrophic bacterium OUR, heterotroph OUR under standard conditions and always
The variation diagram of OUR；
Fig. 2 (a), Fig. 2 (b) are respectively that COD variation and water outlet NH are discharged in temperature-fall period4 +- N and TN variation.
Specific embodiment
Below by accompanying drawings and embodiments, the present invention will be further described.
The present invention determines active sludge microorganism by the breathing map of activated sludge under measurement scene and standard conditions
The situation of suspend mode and loss.
The present invention is based on the methods that breathing map differentiates active sludge microorganism suspend mode and loss, include the following steps:
1) taking certain treatment process is A2Sewage plant sludge without any processing in the sewage plant aerobic tank of O；
2) breathing map test under field condition is carried out to sewage plant activated sludge: keeps anti-with sewage treatment plant in temperature
It answers under the uniform condition of pond, take sewage plant sludge 0.3L and is diluted to 1.2L with tap water, measure status oxygen consumption rate OURs；Later
Mud sample is passed through and stirs 15s, precipitates 10min, supernatant is gone to be settled to 0.6L, is washed mud 3~5 times with PBS buffer solution, it is excellent
It is selected as 3 times；Measure the endogenous oxygen consumption rate OUR of standard of sludgeq；Then with tap water by active sludge intermixture constant volume in reactor
To 1.2L, by being aerated 2~4h, preferably 2h to mud sample；So that activated sludge is entered endogenous respiration state, it is endogenous to measure its
Oxygen consumption rate OURe；
Buffer solution is following components mixed liquor:
A:KH2PO4Concentration is 1.5~2.5mmolL-1；
B:Na2HPO4Concentration is 8~12mmolL-1；
C:NaCl concentration is 135~140mmolL-1；
D:KCl concentration is 2.5~3.0mmolL-1。
3) after step 2), 40~60mg/L of ammonium chloride, preferably 50mg/L are added into sludge；It surveys after adding nitrogen source
Oxygen consumption rate OURen=OURe+OURn；It is eventually adding the anhydrous sodium acetate of 250~350mg/L, preferably 300mg/L；To guarantee
Matrix is sufficient, measures total oxygen consumption rate OURenc=OURe+OURn+OURc.Oxygen consumption rate under available 5 field conditions: status
Oxygen consumption rate OURs, quasi- endogenous oxygen consumption rate OURq, endogenous oxygen consumption rate OURePlus oxygen consumption rate OUR after nitrogen sourceenWith total oxygen consumption
Rate OURenc, and make following calculate: OURn=OURen-OURe, OURc=OURenc-OURen；
4) breathing map test under standard conditions is carried out to sewage plant activated sludge: breathes map test under standard conditions and removes
Temperature condition is changed to outside 20 DEG C, remaining process is consistent with map test method is breathed under field condition.Respectively obtain standard conditions
Lower status oxygen consumption rate OUR 's, quasi- endogenous oxygen consumption rate OUR 'q, endogenous oxygen consumption rate OUR 'ePlus oxygen consumption rate after nitrogen source
OUR’enWith total oxygen consumption rate OUR 'enc；And make following calculate: OUR 'n=OUR 'en-OUR’e, OUR 'c=OUR 'enc-OUR’en。
Measurement data and calculating are shown in Table 1.
Oxygen consumption rate under 1 scene of table and standard conditions
5) according to autotrophic bacterium oxygen consumption rate OUR under field conditionn, heterotroph oxygen consumption rate OURcAnd total oxygen consumption rate
OURencWith autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsn, heterotroph oxygen consumption rate OUR 'cAnd total oxygen consumption rate OUR 'enc's
Variation respectively draw field condition under with autotrophic bacterium oxygen consumption rate, heterotroph oxygen consumption rate and total oxygen consumption rate under standard conditions
Variation diagram, see shown in Fig. 1 (a), Fig. 1 (b), Fig. 1 (c).
This experiment uses sbr reactor device, and it is A that seed sludge, which is derived from Xi'an treatment process,2The sewage plant aerobic tank of O.Instead
Answering device dischargeable capacity is 7L, and water-filling ratio is 1/3.The running temperature of reactor is controlled by water-bath layer external temperature controller
System.The reactor cycle of operation is 8h, operating condition in each period are as follows: water inlet 0.2h → aeration stirring 6h → precipitating 1.6h → row
Water 0.2h.
Experimental method uses respirometry, and it is dense to monitor the oxygen balance in constant temperature aerobic reactor by intellectualized analysis platform
Degree, is calculated oxygen absorption efficiency, and the measurement of different strain respiratory rate is realized in experiment by adding different substrates.Entire analysis
Process is carried out automatically controlling by instrument by PLC, is kept aeration quantity stabilization, is stirred evenly.Measure spirogram time spectrum temperature with
Reactor running temperature is defined as field condition breathing map when identical；The temperature for measuring spirogram time spectrum is controlled determines at 20 DEG C
Justice is that standard conditions breathe map.
Embodiment analysis:
Cool down in experimentation, what field condition breathing map reflected is the activity of microorganism under field condition, with water outlet
Water quality has substantial connection, can characterize the sewage treatment capacity of active sludge microorganism under certain condition.And standard conditions are exhaled
Inhale map reflection is the intrinsic characteristic of active sludge microorganism.As seen from the figure, gradually decreasing with temperature, field condition
Autotrophic bacterium oxygen consumption rate OURn, heterotroph oxygen consumption rate OURcWith total oxygen consumption rate OURencIt is gradually reduced.Illustrate temperature reduction
The activity that will lead to microorganism under field condition reduces, so as to cause sewage treatment capacity decline.
Standard conditions breathing map can be divided into three phases, first stage it can be seen from Fig. 1 (a), (b), (c)
Domestication culture to the stabilization sub stage, second stage is down to 10 DEG C of standard conditions by 20 DEG C for temperature and breathes maps not under the conditions of being 20 DEG C
Significant change stage, phase III are down to 5 DEG C by 10 DEG C for temperature, and standard breathing map starts the reduction stage.
The standard conditions breathing map of second stage is apparently higher than the first stage, this is because running in this stage reactors
Temperature gradually lowers, and 20 DEG C of measuring temperature of standard conditions breathing map are a kind of heating stimulation for microorganism, temperature
The unexpected raising of degree can make active sludge microorganism generate stress reaction, so that the oxygen consumption rate of microorganism increases.Second
The equal kept stable of standard conditions oxygen consumption rate in stage, and field condition oxygen consumption rate is gradually reduced, and is illustrated herein
The intrinsic activity of active microorganism does not reduce in stage, only at the scene under the conditions of sludge activity be gradually reduced, part
Microorganism is since temperature has been lowered into dormant state, if the quick activity recovery of activated sludge can be made by increasing temperature at this time.By
This explanation when field condition breathing map reduce and standard conditions breathing map significant change does not occur when, part microorganism into
Enter dormant state, the activity of quick activating microorganisms can be made by increasing temperature at this time.
The standard conditions breathing map of phase III is significantly less than second stage.By Fig. 2 (a), Fig. 2 (b) it is found that third rank
Section sludge loses nitrification ability substantially, and organic matter removal ability also sharply deteriorates, and microorganism is in this stage known to material balance
Slow growth, autotrophic bacterium even without growth, but due to timing spoil disposal cause active sludge microorganism especially autotrophic bacterium occur
It is lost, microorganism activity recovery can not also be made by increasing temperature at this time, therefore standard conditions breathing map is obviously reduced.Thus illustrate
When standard conditions oxygen consumption rate is obviously reduced, illustrate that being lost occurs in microorganism.
During second stage temperature is down to 10 DEG C by 20 DEG C, autotrophic bacterium oxygen consumption rate OUR under field conditionnWith heterotrophism
Bacterium oxygen consumption rate OURcThe range of decrease be respectively 40% and 25% or so, the standard conditions autotrophic bacterium oxygen consumption rate OUR ' of phase IIIn
31.48% is reduced compared to second stage, and standard conditions heterotroph oxygen consumption rate OUR 'c12.17% is reduced, is thus illustrated
Heterotroph is significantly stronger than autotrophic bacterium to the adaptability of low temperature, and under cryogenic conditions autotrophic bacterium more holds compared with heterotroph in activated sludge
Suspend mode easily occurs and is lost.
6) it can be seen from the figure that in the continuous data of multiple groups:
As autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnStablize constant, and autotrophic bacterium oxygen consumption rate OUR under field conditionn
When reduction, illustrate that there are suspend mode situations for autotrophic bacterium in activated sludge, while if autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsn
With autotrophic bacterium oxygen consumption rate OUR under field conditionnDifference is bigger, then the autotrophy bacterium amount of suspend mode is more in activated sludge；Work as standard
Under the conditions of heterotroph oxygen consumption rate OUR 'cStablize constant, and heterotroph oxygen consumption rate OUR under field conditioncWhen reduction, illustrate to live
There are suspend mode situations for heterotroph in property sludge；If heterotroph oxygen consumption rate OUR ' under standard conditions simultaneouslycUnder field condition
Heterotroph oxygen consumption rate OURcDifference is bigger, and the heterotrophism bacterium amount of suspend mode in activated sludge is more.
7) in the continuous data of multiple groups: as autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnWhen being obviously reduced, illustrate certainly
Bacteria starts to be lost, while autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnWhat is reduced is more, and autotrophy bacterium amount is lost more；When
Heterotroph oxygen consumption rate OUR ' under standard conditionscWhen being obviously reduced, illustrate that heterotroph starts to be lost in activated sludge, while standard
Under the conditions of heterotroph oxygen consumption rate OUR 'cWhat is reduced is more, and heterotrophism bacterium amount is lost more in activated sludge.
The method of the present invention can breathe map according to activated sludge and effectively differentiate active sludge microorganism suspend mode and be lost
State makes the technical work personnel of sewage plant understand the processing capacity of current active sludge, adjusts operating parameter in time, is winter
The stable operation and operation regulation of sewage treatment plant provide guiding opinion under cryogenic conditions.
The above is only embodiment of the present invention for invention application, can make those skilled in the art more comprehensively
Understanding the present invention, but not any restrictions are done to the present invention.According to technical solution of the present invention, above-described embodiment can be enumerated very much
Example.Range given by all technical solutions according to the present invention and any simple modification made to the above embodiment and change
More, the protection scope of technical solution of the present invention is belonged to.Largely the experimental results showed that, proposed in claims of the present invention
Range, can reach the purpose of the present invention.
Claims (6)
1. a kind of method for differentiating active sludge microorganism suspend mode and loss based on breathing map, which is characterized in that including following
Step:
1) sludge sewage is taken；
2) breathing map test under continuous 2~7 days field conditions is carried out to activated sludge, respectively obtains status under field condition and consumes
Oxygen rate OURs, quasi- endogenous oxygen consumption rate OURq, endogenous oxygen consumption rate OURePlus oxygen consumption rate OUR after nitrogen sourceenWith total oxygen consumption speed
Rate OURencMeasurement data；
3) it is calculated by measurement data
OURn=OURen-OURe,
OURc=OURenc-OURen；
Wherein, OURnFor autotrophic bacterium oxygen consumption rate under field condition；OURcFor heterotroph oxygen consumption rate under field condition；
4) breathing map test under continuous 2~7 days standard conditions is carried out to sewage plant activated sludge, respectively obtained under standard conditions
Status oxygen consumption rate OUR 's, quasi- endogenous oxygen consumption rate OUR 'q, endogenous oxygen consumption rate OUR 'ePlus oxygen consumption rate OUR ' after nitrogen sourceen
With total oxygen consumption rate OUR 'enc；
5) it is calculated by measurement data
OUR’n=OUR 'en-OUR’e,
OUR’c=OUR 'enc-OUR’en；
Wherein, OUR 'nFor autotrophic bacterium oxygen consumption rate under standard conditions；OUR'cFor heterotroph oxygen consumption rate under standard conditions；
6) in the continuous data of multiple groups:
As autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnStablize constant, and autotrophic bacterium oxygen consumption rate OUR under field conditionnReduce
When, then there are suspend mode situations for activated sludge autotrophic bacterium；If autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnUnder field condition
Autotrophic bacterium oxygen consumption rate OURnDifference is bigger, then activated sludge autotrophic bacterium suspend mode amount is more；
As heterotroph oxygen consumption rate OUR ' under standard conditionscStablize constant, and heterotroph oxygen consumption rate OUR under field conditioncReduce
When, then there are suspend mode situations for activated sludge heterotroph；If heterotroph oxygen consumption rate OUR ' under standard conditionscUnder field condition
Heterotroph oxygen consumption rate OURcDifference is bigger, then activated sludge heterotroph suspend mode amount is more；
7) in the continuous data of multiple groups:
As autotrophic bacterium oxygen consumption rate OUR ' under standard conditionsnWhen being obviously reduced, then activated sludge autotrophic bacterium starts to be lost, same to markers
Autotrophic bacterium oxygen consumption rate OUR ' under the conditions of standardnWhat is reduced is more, and activated sludge autotrophy bacterium amount is lost more；
As heterotroph oxygen consumption rate OUR ' under standard conditionscWhen being obviously reduced, then activated sludge heterotroph starts to be lost, same to markers
Heterotroph oxygen consumption rate OUR ' under the conditions of standardcWhat is reduced is more, and activated sludge heterotrophism bacterium amount is lost more；
The detailed process of map test is breathed in step 2) under field condition are as follows:
In the case where temperature keeps with sewage plant reaction tank uniform condition, sludge sewage is taken, and with the ratio of volume ratio 1:3
Example addition tap water dilution, measures status oxygen consumption rate OUR under field conditions；By activated sludge sample by stirring, precipitating,
1/2 volume removed supernatant, be settled to activated sludge dilution washes mud with PBS buffer solution, measures the endogenous oxygen consumption of standard of sludge
Rate OURq；Active sludge intermixture is settled to the original volume of activated sludge dilution, aeration, activity by addition tap water later
Sludge enters endogenous respiration state, measures its endogenous oxygen consumption rate OURe；Add again by certain mass than ammonium chloride, measurement is added
Oxygen consumption rate OUR after nitrogen sourceen=OURe+OURn；Enough anhydrous sodium acetates are eventually adding, guarantee that matrix is sufficient, measure total consumption
Oxygen rate OURenc=OURe+OURn+OURc；
In step 3), the detailed process of map test is breathed under standard conditions are as follows:
Under the conditions of temperature is 20 DEG C, sludge sewage is taken, and dilute with the ratio of volume ratio 1:3 addition tap water
It releases, measures status oxygen consumption rate OUR 's；Activated sludge sample is passed through and stirs, precipitate, removing supernatant, being settled to activated sludge
1/2 volume of dilution washes mud with PBS buffer solution, measures the endogenous oxygen consumption rate OUR ' of standard of sludgeq；It adds later originally
Active sludge intermixture is settled to the original volume of activated sludge dilution by water, and aeration, activated sludge enters endogenous respiration state,
Measure its endogenous oxygen consumption rate OUR 'e；Again by certain mass than ammonium chloride, oxygen consumption rate OUR ' after measurement plus nitrogen source is addeden=
OUR’e+OUR’n；Enough anhydrous sodium acetates are eventually adding, guarantees that matrix is sufficient, measures total oxygen consumption rate OUR 'enc=OUR 'e+
OUR’n+OUR’c。
2. a kind of method that active sludge microorganism suspend mode and loss are differentiated based on breathing map described in accordance with the claim 1,
It is characterized in that, buffer solution is following components mixed liquor:
A:KH2PO4Concentration is 1.5~2.5mmolL-1；
B:Na2HPO4Concentration is 8~12mmolL-1；
C:NaCl concentration is 135~140mmolL-1；
D:KCl concentration is 2.5~3.0mmolL-1。
3. a kind of method that active sludge microorganism suspend mode and loss are differentiated based on breathing map according to claim 2,
It is characterized in that, being washed mud 3~5 times with PBS buffer solution.
4. a kind of method that active sludge microorganism suspend mode and loss are differentiated based on breathing map described in accordance with the claim 1,
It is characterized in that, active sludge intermixture is settled to the original volume of activated sludge dilution by addition tap water, it is aerated 2~4h.
5. a kind of method that active sludge microorganism suspend mode and loss are differentiated based on breathing map described in accordance with the claim 1,
It is characterized in that, ammonium chloride is added according to 40~60mg/L.
6. a kind of method that active sludge microorganism suspend mode and loss are differentiated based on breathing map described in accordance with the claim 1,
It is characterized in that, anhydrous sodium acetate is added according to 250~350mg/L.
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| CN108483621B (en) * | 2018-04-17 | 2020-04-21 | 西安建筑科技大学 | Optimal region for determining multi-stage water inlet process of sewage treatment and adjusting method |
| CN108675439B (en) * | 2018-05-18 | 2020-08-28 | 西安建筑科技大学 | Method for selecting regulation and control measures in sewage treatment process |
| CN109001435B (en) * | 2018-07-16 | 2022-03-29 | 西安建筑科技大学 | Method for realizing accident early warning and management optimization of sewage treatment plant by adopting breathing pattern |
| CN109207616A (en) * | 2018-11-14 | 2019-01-15 | 北京工业大学 | A kind of method of dead bacterium or suspend mode bacterium in detection activated sludge |
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| EP1466869A1 (en) * | 2003-04-08 | 2004-10-13 | Gunnar Demoulin | Apparatus and process for wastewater purification |
| CN201903539U (en) * | 2010-12-03 | 2011-07-20 | 北京工业大学 | Frequency-converting speed-regulating fast online detecting device for specific oxygen uptake rate |
| US9475714B2 (en) * | 2011-07-04 | 2016-10-25 | Biological Petroleum Cleaning Ltd. | Method and system for treating waste material |
| CN105439285B (en) * | 2015-12-04 | 2019-01-08 | 中国科学院生态环境研究中心 | A kind of regulation method of sewage treatment |
| CN105645576B (en) * | 2016-01-14 | 2018-04-10 | 西安建筑科技大学 | A kind of judgement of Activated Sludge Growth state and regulation and control method |
| CN106082430B (en) * | 2016-07-26 | 2019-10-18 | 尚川（北京）水务有限公司 | A kind of aeration control system and aeration control method |
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