CN106729923A - 一种人工敷料的制备方法 - Google Patents

一种人工敷料的制备方法 Download PDF

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CN106729923A
CN106729923A CN201610990333.2A CN201610990333A CN106729923A CN 106729923 A CN106729923 A CN 106729923A CN 201610990333 A CN201610990333 A CN 201610990333A CN 106729923 A CN106729923 A CN 106729923A
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    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract

本发明公开了一种人工敷料的制备方法。对该纤维膜进行抑菌试验，含有氧化石墨烯的所述聚乳酸纳米纤维膜出现抑菌圈，抑菌率大于94％，因此其可以在伤口敷料领域进行应用。

Description

一种人工敷料的制备方法
技术领域
本发明涉及医疗材料技术领域，尤其涉及一种人工敷料的制备方法。
背景技术
通过静电纺丝技术制备的纳米纤维在生物医学领域得到了广泛的应用，比如：药物控制释放、组织工程支架、伤口敷料、医疗保健、仿生材料等。
采用静电纺丝制备的多孔纤维膜具有可控的纤维直径、较高的比表面积、多孔结构、一定的力学强度等优点，符合仿生人体细胞外基质的要求，广泛应用于多孔支架材料的制备，成为组织工程领域宠儿。
静电纺丝纤维较大的比表面积能够快速挥发溶剂，这对药物的均匀分散很有利，提高药物的利用率。静电纺丝因为具有载药量高、药物释放可控等优点而在药物传输应用中有着很好的前景。
吉林大学以添加硅胶银的PLGA为纺丝原料对其进行静电纺丝制备复合人工敷料，实验发现其促进皮肤伤口愈合的效果很好。随后大最实验结果表明通过静电纺丝方法制备的复合人工敷料能对伤口进行止血提供保护作用，并且促进伤口的愈合。静电纺丝制备的纤维轻薄孔隙率高，能够与伤口充分接触，保护创面并增强细胞修复，所以在伤口敷料方面有着绝对的发展优势。
创伤是不可忽视的一类疾病，伤口敷料一直是生活以及医药领域的必需品。传统敷料已难以满足人们的要求，所以近年来，可降解性合成聚合物纳米纤维敷料成为大家研究的热点。聚乳酸(PLA)有着难以比拟的优良生物降解性、生物相容性和一定的强度，广泛应用于生物民药领域。近来，有关石墨烯的抗菌性以及其细胞毒性的研究很多，结果表明石墨烯具冇很好的抑菌效果且对细胞无毒性，冋时氧化石墨烯有着很好的亲水性能，这为石墨烯在生物医药领域的应用提供了坚实的理论基础。目前为止，尚未发现有关将石墨烯加入聚乳酸进行静电纺丝，应用于生物伤口敷料的相关报道。
发明内容
本发明的目的在于提出一种人工敷料的制备方法，能够使得敷料的抑菌效果增强。
为达此目的，本发明采用以下技术方案：
一种人工敷料的制备方法，包括：
(1)将2-5重量份的鳞片石墨，300-500重量份的浓硫酸和30-60重量份的磷酸混合均匀，缓慢加入15-20重量份的高锰酸钾，于45-55℃下搅拌9-18小时；
(2)在0-5℃下，向混合物中加入300-500重量份的去离子水，和20-50重量份的10-30wt％浓度的双氧水，并搅拌5-10分钟；
(3)分离并先后用去离子水、10-30wt％浓度的盐酸和无水乙醇洗涤产物，真空干燥得到氧化石墨烯；
(4)将所述氧化石墨烯分散于二甲基甲酰胺中，形成2-5mg/mL的氧化石墨烯溶液；
(5)按聚乳酸:氧化石墨烯为1000:(2-5)的重量比，将聚乳酸与氧化石墨烯混合均匀，加入二甲基甲酰胺，搅拌至少12小时，获得氧化石墨烯纺丝液；
(6)对所述纺丝液进行静电纺丝，获得氧化石墨烯掺杂的聚乳酸纳米纤维膜。
对静电纺丝获得的纤维膜进行红外光谱以及拉曼光谱测试，发现氧化石墨烯的存在，证明本申请的方法制备得到了目标产物。
对该纤维膜进行抑菌试验，含有氧化石墨烯的所述聚乳酸纳米纤维膜出现抑菌圈，抑菌率大于94％，因此其可以在伤口敷料领域进行应用。
具体实施方式
下面通过具体实施方式来进一步说明本发明的技术方案。
实施例1
一种人工敷料的制备方法，包括：
(1)将2重量份的鳞片石墨，300重量份的浓硫酸和30重量份的磷酸混合均匀，缓慢加入15重量份的高锰酸钾，于45℃下搅拌9小时；
(2)在0℃下，向混合物中加入300重量份的去离子水，和20重量份的10％浓度的双氧水，并搅拌5分钟；
(3)分离并先后用去离子水、10wt％浓度的盐酸和无水乙醇洗涤产物，真空干燥得到氧化石墨烯；
(4)将所述氧化石墨烯分散于二甲基甲酰胺中，形成2mg/mL的氧化石墨烯溶液；
(5)按聚乳酸:氧化石墨烯为1000:2-的重量比，将聚乳酸与氧化石墨烯混合均匀，加入二甲基甲酰胺，搅拌至少12小时，获得氧化石墨烯纺丝液；
(6)对所述纺丝液进行静电纺丝，获得氧化石墨烯掺杂的聚乳酸纳米纤维膜。
实施例2
一种人工敷料的制备方法，包括：
(1)将5重量份的鳞片石墨，500重量份的浓硫酸和60重量份的磷酸混合均匀，缓慢加入20重量份的高锰酸钾，于55℃下搅拌18小时；
(2)在5℃下，向混合物中加入500重量份的去离子水，和50重量份的30wt％浓度的双氧水，并搅拌10分钟；
(3)分离并先后用去离子水、30wt％浓度的盐酸和无水乙醇洗涤产物，真空干燥得到氧化石墨烯；
(4)将所述氧化石墨烯分散于二甲基甲酰胺中，形成5mg/mL的氧化石墨烯溶液；
(5)按聚乳酸:氧化石墨烯为1000:2的重量比，将聚乳酸与氧化石墨烯混合均匀，加入二甲基甲酰胺，搅拌至少12小时，获得氧化石墨烯纺丝液；
(6)对所述纺丝液进行静电纺丝，获得氧化石墨烯掺杂的聚乳酸纳米纤维膜。
实施例3
一种人工敷料的制备方法，包括：
(1)将3重量份的鳞片石墨，400重量份的浓硫酸和40重量份的磷酸混合均匀，缓慢加入20重量份的高锰酸钾，于50℃下搅拌12小时；
(2)在0℃下，向混合物中加入400重量份的去离子水，和30重量份的20wt％浓度的双氧水，并搅拌8分钟；
(3)分离并先后用去离子水、20wt％浓度的盐酸和无水乙醇洗涤产物，真空干燥得到氧化石墨烯；
(4)将所述氧化石墨烯分散于二甲基甲酰胺中，形成3mg/mL的氧化石墨烯溶液；
(5)按聚乳酸:氧化石墨烯为1000:3的重量比，将聚乳酸与氧化石墨烯混合均匀，加入二甲基甲酰胺，搅拌至少12小时，获得氧化石墨烯纺丝液；
(6)对所述纺丝液进行静电纺丝，获得氧化石墨烯掺杂的聚乳酸纳米纤维膜。
对实施例1-3制备得到的纤维膜进行抑菌试验，含有氧化石墨烯的所述聚乳酸纳米纤维膜出现抑菌圈，抑菌率大于94％，因此其可以在伤口敷料领域进行应用。

Claims (1)

1.一种人工敷料的制备方法，包括：
(1)将2-5重量份的鳞片石墨，300-500重量份的浓硫酸和30-60重量份的磷酸混合均匀，缓慢加入15-20重量份的高锰酸钾，于45-55℃下搅拌9-18小时；
(2)在0-5℃下，向混合物中加入300-500重量份的去离子水，和20-50重量份的10-30wt％浓度的双氧水，并搅拌5-10分钟；
(3)分离并先后用去离子水、10-30wt％浓度的盐酸和无水乙醇洗涤产物，真空干燥得到氧化石墨烯；
(4)将所述氧化石墨烯分散于二甲基甲酰胺中，形成2-5mg/mL的氧化石墨烯溶液；
(5)按聚乳酸:氧化石墨烯为1000:(2-5)的重量比，将聚乳酸与氧化石墨烯混合均匀，加入二甲基甲酰胺，搅拌至少12小时，获得氧化石墨烯纺丝液；
(6)对所述纺丝液进行静电纺丝，获得氧化石墨烯掺杂的聚乳酸纳米纤维膜。
CN201610990333.2A 2016-11-10 2016-11-10 一种人工敷料的制备方法 Withdrawn CN106729923A (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109137264A (zh) * 2018-07-07 2019-01-04 东莞市联洲知识产权运营管理有限公司 一种采用静电纺丝制备抗菌医用敷料的方法
CN114108184A (zh) * 2021-11-22 2022-03-01 华中科技大学 可降解的石墨烯复合静电纺丝纤维薄膜、制备方法及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199809A (zh) * 2011-04-02 2011-09-28 中国科学院长春应用化学研究所 医用敷料及其制备方法
CN105908374A (zh) * 2016-06-23 2016-08-31 盐城工业职业技术学院 一种多孔纳米纤维膜的制备方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199809A (zh) * 2011-04-02 2011-09-28 中国科学院长春应用化学研究所 医用敷料及其制备方法
CN105908374A (zh) * 2016-06-23 2016-08-31 盐城工业职业技术学院 一种多孔纳米纤维膜的制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109137264A (zh) * 2018-07-07 2019-01-04 东莞市联洲知识产权运营管理有限公司 一种采用静电纺丝制备抗菌医用敷料的方法
CN114108184A (zh) * 2021-11-22 2022-03-01 华中科技大学 可降解的石墨烯复合静电纺丝纤维薄膜、制备方法及应用

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Application publication date: 20170531