CN106729615A - A kind of antibacterial biological film and preparation method thereof - Google Patents
A kind of antibacterial biological film and preparation method thereof Download PDFInfo
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- CN106729615A CN106729615A CN201611037500.8A CN201611037500A CN106729615A CN 106729615 A CN106729615 A CN 106729615A CN 201611037500 A CN201611037500 A CN 201611037500A CN 106729615 A CN106729615 A CN 106729615A
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- antibacterial
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- pectin
- antibacterial peptide
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/732—Pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7015—Drug-containing film-forming compositions, e.g. spray-on
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a kind of antibacterial biological film and preparation method thereof, first using antibacterial peptide amidated pectin, the pectin of antibacterial peptide modification is obtained；Then the pectolysis antibacterial peptide modified, obtains the pectin solution of antibacterial peptide modification, and it is mixed with collagen solution, is homogenized, and obtains film forming solution；Finally the film forming solution is prepared to form antibacterial biological film.The present invention carries out amidatioon using antibacterial peptide to pectin, then meets with collagen and obtains antibacterial biological film so that the antibacterial biological film have efficiently, wide spectrum the characteristics of, with wide Research Prospects and practical value.
Description
Technical field
The present invention relates to field of medical technology, and in particular to a kind of antibacterial biological film and preparation method thereof.
Background technology
Antibacterial peptide is the important composition composition of organism innate immune system, is various different with antibacterial activity alkalescence
The general designation of peptide molecule.Antibacterial peptide have efficiently, broad spectrum antibiotic activity, can quick killing target, mainly may act on leather orchid
Family name's negative bacteria, gram-positive bacterium, fungi, parasite etc..Antibacterial peptide also has potential antitumor activity, and can add
The function such as fast immunity of organism and wound healing process, makes it possible to turn into the one kind for replacing conventional antibiotic and antineoplastic newly
Type medicine.
But at present, existing antibacterial biological film generally uses the chitosan material with medical functions such as antibacterial, anti-inflammatory
Prepare, but shitosan is easily degraded, so obtaining antibacterial biological film has unstable problem of easy degradation.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of antibacterial biological film and preparation method thereof.
The technical solution used in the present invention is：
A kind of preparation method of antibacterial biological film, comprises the following steps：
S1：Antibacterial peptide amidated pectin, obtains the pectin of antibacterial peptide modification；
S2：The pectolysis that the antibacterial peptide is modified, obtains the pectin solution of antibacterial peptide modification, by itself and collagen
Solution mixes, homogenizing, obtains film forming solution；
S3：Prepared by the film forming solution to form antibacterial biological film.
Some preferred embodiment in, the S1's concretely comprises the following steps：Antibacterial peptide solution and pectin solution mixing are taken,
Reaction, filtering, taking precipitate washing is dried, wherein, antibacterial peptide and pectin in the antibacterial peptide solution and the pectin solution
Mass ratio is (0.8-5)：1.
Such scheme preferred embodiment in, pectin is HM.
Such scheme preferred embodiment in, it is anti-in 10-15 DEG C after taking antibacterial peptide solution and pectin solution mixing
Answer 1-3h.
Such scheme preferred embodiment in, washing in the S1 include use alcoholic solution washing, pH for
The alcoholic solution washing of 2-5 and alcoholic solution wash three steps.
Some preferred embodiment in, the S2's concretely comprises the following steps：The pectin that the antibacterial peptide is modified is dissolved in
In acetic acid solution, glycerine is added, obtain the pectin solution of antibacterial peptide modification, it is mixed with collagen solution, be homogenized, adjusted
Section pH to 3-6.5, obtains film forming solution.
Such scheme preferred embodiment in, in the film forming solution antibacterial peptide modification pectin and collagen
Mass ratio be 1：(0.3-0.8).
Such scheme preferred embodiment in, the fruit of antibacterial peptide modification in the pectin solution of antibacterial peptide modification
Glue is 1 with the mass ratio of glycerine：(1-0.1).
Such scheme preferred embodiment in, the concentration of the acetic acid solution is 0.5-2wt%.
Additionally, present invention also offers the antibacterial life prepared using the preparation method of antibacterial biological film as described above
Thing film.
The beneficial effects of the invention are as follows：
For the current unstable problem of easy degradation of antibacterial biological film, the invention provides a kind of antibacterial biological film and its
Preparation method, first using antibacterial peptide amidated pectin, obtains the pectin of antibacterial peptide modification；Then the antibacterial peptide is modified
Pectolysis, obtains the pectin solution of antibacterial peptide modification, and it is mixed with collagen solution, is homogenized, and obtains film forming solution；Most
Afterwards the film forming solution is prepared to form antibacterial biological film.Antibacterial peptide has obvious amphipathic, and its N-terminal molecule is rich in hydrophily
Amino acid residue, particularly lysine and arginine, and C-terminal then contains more hydrophobic amino acid residues, and major part is by acyl
Amination, and amidatioon plays an important roll to antibacterial activity, and antibacterial peptide can be protected from the degradation of enzyme so as to prolong
The affinity of half life or increase peptide and acceptor in vivo long；Pectin is a kind of complicated polysaccharide polymer, can be in alkaline bar
Amidation process is carried out under part and prepares amidated pectin, amidated pectin can be used in pharmaceuticals industry be used as to treat anti-diarrhea drug
Constituent and promotion wound healing etc.；Collagen is a kind of biological polymer substance, with high-hydrophilic, low toxicity
The advantages of property, poor antigen, good biocompatibility, complete biodegradability；The present invention is entered using antibacterial peptide to pectin
Row amidatioon, then meets with collagen and obtains antibacterial biological film so that the antibacterial biological film has the efficient, spy of wide spectrum
Point, with wide Research Prospects and practical value.
Specific embodiment
Embodiment 1：
The concentrate that pectin is obtained during HM is produced is taken, makes its concentration of pectin 2%；By antibacterial peptide and 95%
Alcohol mixing, obtain antibacterial peptide solution, control antibacterial peptide concentration is 7%；Above-mentioned antibacterial peptide solution and pectin solution are pressed
The ratio mixing of volume ratio 3: 1,12 DEG C of reaction 2h；Reaction takes out sediment after terminating, and filters, 60% ethanol wash of filter cake,
Washed with the 60% of pH3.5 alcoholic solution again, then use 60% ethanol wash；Filter cake dry, pulverize at 60 DEG C, antibacterial peptide
The pectin of modification.
By concentration be 3wt% antibacterial peptides modification pectolysis concentration be 1wt% acetic acid solutions in, then thereto plus
Enter glycerine, the dosage of glycerine is the 25% of the quality of the pectin of antibacterial peptide modification, and the pectin for preparing antibacterial peptide modification is molten
Liquid.By concentration for 10wt% collagens are dissolved in distilled water and add glycerine, the dosage of glycerine is the matter of collagen
The 25% of amount, obtains collagen solution.The pectin solution and collagen solution that antibacterial peptide is modified mix, and reach antibacterial peptide
The pectin of modification is 1 with the mass ratio of collagen：0.6.The compound mixture for obtaining with homogenizer (PT10-35,
Kinematica) it is homogenized 60 seconds with the rotating speed of 3000rpm, pH is adjusted to 5 with sodium hydroxide solution, obtains film forming solution.
Film forming solution is filtered by nylon mesh, to remove insoluble residue and deaerate under vacuo.It is again that film forming is molten
On the glass plate that flat Teflon is coated with, area is 260x260mm to liquid curtain coating, to reach about 70 μm of homogeneous film thickness
Degree.After 25 DEG C of dryings 2 days, dry film is peeled off from plate, obtain antibacterial biological film, and cut into slices for analyzing.
Antibacterial effect to antibacterial biomembrane is analyzed, and obtains experimental result such as table 1, measures the antibacterial effect of biomembrane
Method be CLSI standard methods, i.e., (pectin is directly combined the life of preparation to blank original film without antibacterial peptide modification with collagen
Thing film) it is cut into the disk (diameter 2cm) of formed objects respectively with the antibacterial biological film for preparing, it is placed in agar plates and trains
Support 24 hours, 37 DEG C of temperature, strain is Escherichia coli (Escherichiacoli, numbering：GIM1.355, is purchased from the micro- life in Guangdong Province
Wu Suo DSMZs).Sample boundary area diameter is measured after the completion of culture, clear area is antibacterial area, does not have strain to give birth to
It is long.Clear area then represents greatly good anti-bacterial effect, otherwise represents that antibacterial effect is poor.As can be seen from Table 1, antibacterial biological of the invention
Film has obvious antibacterial effect.
The antibacterial effect of the antibacterial biological film of table 1
Embodiment 2：
The present embodiment is substantially the same manner as Example 1, and difference is：Concentration of pectin is 2% in the pectin solution；Will
Alcohol of the antibacterial peptide with 95% mixes, and obtains antibacterial peptide solution, and control antibacterial peptide concentration is 6%；Reaction takes out precipitation after terminating
Thing, filtering, 60% ethanol wash of filter cake, then washed with the 60% of pH2 alcoholic solution, then use 60% ethanol wash；Will
Above-mentioned antibacterial peptide solution and pectin solution by volume 3: 1 ratio mixing, 10 DEG C reaction 3h；It is 3wt% antibacterials by concentration
Then the pectolysis of peptide modification is added thereto to glycerine in concentration is 0.5wt% acetic acid solutions, and the dosage of glycerine is anti-
The quality of the pectin of bacterium peptide modification, prepares the pectin solution of antibacterial peptide modification；Antibacterial peptide modification in the film forming solution
Pectin is 1 with the mass ratio of collagen：0.3, the pH of the film forming solution is 3.
Embodiment 3：
The present embodiment is substantially the same manner as Example 1, and difference is：Concentration of pectin is 5% in the pectin solution；Will
Alcohol of the antibacterial peptide with 95% mixes, and obtains antibacterial peptide solution, and control antibacterial peptide concentration is 1%；Reaction takes out precipitation after terminating
Thing, filtering, 60% ethanol wash of filter cake, then washed with the 60% of pH5 alcoholic solution, then use 60% ethanol wash；Will
Above-mentioned antibacterial peptide solution and pectin solution by volume 1: 1 ratio mixing, 15 DEG C reaction 1h；It is 3wt% antibacterials by concentration
Then the pectolysis of peptide modification is added thereto to glycerine in concentration is 2wt% acetic acid solutions, and the dosage of glycerine is antibacterial
The 10% of the quality of the pectin of peptide modification；The pectin of antibacterial peptide modification and the mass ratio of collagen are 1 in the film forming solution：
0.8, the pH of the film forming solution is 6.5.
Claims (10)
1. a kind of preparation method of antibacterial biological film, it is characterised in that comprise the following steps：
S1：Antibacterial peptide amidated pectin, obtains the pectin of antibacterial peptide modification；
S2：The pectolysis that the antibacterial peptide is modified, obtains the pectin solution of antibacterial peptide modification, by itself and collagen solution
Mixing, homogenizing, obtains film forming solution；
S3：Prepared by the film forming solution to form antibacterial biological film.
2. the preparation method of antibacterial biological film according to claim 1, it is characterised in that the S1's concretely comprises the following steps：
Take antibacterial peptide solution and pectin solution mixing, reaction, filtering, taking precipitate washing, dry, wherein, the antibacterial peptide solution and
Antibacterial peptide is with the mass ratio of pectin in the pectin solution（0.8-5）：1.
3. the preparation method of antibacterial biological film according to claim 2, it is characterised in that pectin is HM.
4. the preparation method of the antibacterial biological film according to Claims 2 or 3, it is characterised in that take antibacterial peptide solution and fruit
After sol solution mixing, 1-3h is reacted in 10-15 DEG C.
5. the preparation method of the antibacterial biological film according to Claims 2 or 3, it is characterised in that the washing bag in the S1
Include and use alcoholic solution washing, three steps are washed in the alcoholic solution washing that pH is 2-5 and alcoholic solution.
6. the preparation method of antibacterial biological film according to claim 1, it is characterised in that the S2's concretely comprises the following steps：
The pectin that the antibacterial peptide is modified is dissolved in acetic acid solution, glycerine is added, the pectin solution of antibacterial peptide modification is obtained, by it
Mix with collagen solution, be homogenized, pH is to 3-6.5 for regulation, obtains film forming solution.
7. the preparation method of antibacterial biological film according to claim 6, it is characterised in that antibacterial peptide in the film forming solution
The pectin of modification is 1 with the mass ratio of collagen：（0.3-0.8）.
8. the preparation method of the antibacterial biological film according to claim 6 or 7, it is characterised in that the antibacterial peptide modification
The pectin of antibacterial peptide modification and the mass ratio of glycerine are 1 in pectin solution：（1-0.1）.
9. the preparation method of the antibacterial biological film according to claim 6 or 7, it is characterised in that the acetic acid solution it is dense
It is 0.5-2wt% to spend.
10. the antibacterial biological that a kind of preparation method of the antibacterial biological film described in use claim any one of 1-9 is prepared
Film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037500.8A CN106729615A (en) | 2016-11-23 | 2016-11-23 | A kind of antibacterial biological film and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037500.8A CN106729615A (en) | 2016-11-23 | 2016-11-23 | A kind of antibacterial biological film and preparation method thereof |
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| CN106729615A true CN106729615A (en) | 2017-05-31 |
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| CN201611037500.8A Pending CN106729615A (en) | 2016-11-23 | 2016-11-23 | A kind of antibacterial biological film and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110997004A (en) * | 2017-06-08 | 2020-04-10 | S·I·切尔内什 | Method for disrupting and preventing the formation of bacterial biofilms using insect antimicrobial peptide complexes |
| CN115490894A (en) * | 2022-09-27 | 2022-12-20 | 巢湖学院 | Preparation method of pectin/ZnO/eugenol antibacterial active food packaging film |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1531419A (en) * | 2001-02-28 | 2004-09-22 | ʵ | In-situ gel formation of pectins |
-
2016
- 2016-11-23 CN CN201611037500.8A patent/CN106729615A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1531419A (en) * | 2001-02-28 | 2004-09-22 | ʵ | In-situ gel formation of pectins |
Non-Patent Citations (5)
| Title |
|---|
| GLADSTONE CHRISTOPHER JAYAKUMAR等: "Preparation of antibacterial collagen–pectin particles for biotherapeutics", 《RSC ADV.》 * |
| HE J等: "design and activity of a "dual-targeted" antimicrobial peptide", 《ANTIMICROB AGENTS》 * |
| MARIA KRATCHANOVA等: "Interaction of pectin with amino acids and other amino compounds in aqueous solution", 《FOOD HYDROCOLLOIDS》 * |
| 杨建等: "抗菌肽改性蚕丝蛋白膜的细胞相容性研究", 《丝绸》 * |
| 陈曼等: "抗菌肽PGG酰胺化合成物的生物学特性研究", 《实用医学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110997004A (en) * | 2017-06-08 | 2020-04-10 | S·I·切尔内什 | Method for disrupting and preventing the formation of bacterial biofilms using insect antimicrobial peptide complexes |
| CN115490894A (en) * | 2022-09-27 | 2022-12-20 | 巢湖学院 | Preparation method of pectin/ZnO/eugenol antibacterial active food packaging film |
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Application publication date: 20170531 |