CN106727775A - A kind of crocodile mouth spends anticancer liquid - Google Patents
A kind of crocodile mouth spends anticancer liquid Download PDFInfo
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- CN106727775A CN106727775A CN201710035397.1A CN201710035397A CN106727775A CN 106727775 A CN106727775 A CN 106727775A CN 201710035397 A CN201710035397 A CN 201710035397A CN 106727775 A CN106727775 A CN 106727775A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a kind of crocodile mouth flower anticancer liquid, prepared using following steps：1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；2), distilled water is cleaned 2 times, is freezed, and is ground into the fragment of 10~20mm, and 50 DEG C are incubated 1~2 hour；3) distilled water of 100~120 times of fresh leaf fragment volume, is added, adds the complex enzyme formulation of 0.02~0.05 times of leaf quality to be digested；4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, linalool to final concentration one thousandth is added；5), 4~8 DEG C of preservations.Crocodile mouth of the invention spends anticancer liquid, and preparation method is simple, and the inhibiting rate to cancer cells such as liver cancer, stomach cancer, breast cancer, lung cancer reaches more than 50%；Long-term taking is not needed, the various complication such as various cardiovascular and cerebrovascular diseases, hypertension, high fat of blood, ephrosis can be effectively controlled, the microcirculation of conditioning each organ of human body, improves metabolic function comprehensively, and nontoxic pair of effect can long-term taking, security reliability.
Description
Technical field
The present invention relates to cancer therapy drug, specially a kind of crocodile mouth flower anticancer liquid.
Background technology
Crocodile mouth spends (Clinacanthus nutans (Burm.f.) Lindau), is commonly called as excellent (sorrow) and escapes that grass, bamboo branch are yellow, turn round sequence
Flower etc., belong to Acanthaceae crocodile mouth flower category, be wide variety of medicinal plant in the conventional medicament of Southeast Asia, be distributed in South East Asia Mainland,
The ground such as the ground such as Guangxi, Guangdong, Yunnan, Hainan of the Malay Peninsula and China's Mainland, and Malaysia, Thailand.Crocodile mouth flower is made
Bitten for a kind of traditional medicinal material is used to treating stress, diabetes, rheumatoid arthritis, snake, hepatitis and antiviral
Deng, wherein, the extract of crocodile mouth Paphio-pedilum micranthum Tang et Wang can treat bite by mosquitos as antiphlogistic, and skin bleb is treated as remedial measure
With the varicella zoster of viral lesion.Additionally, crocodile mouth spend in chlorophyll a and chlorophyll b derivative have anti-herpesvirus
Activity
With the development of modern medicine, researcher has found that crocodile mouth flower also has antitumaous effect and therefore extremely medical research people
The attention of member, but, current crocodile mouth flower anticancer component and the mechanism of action are still not clear, not on having using crocodile mouth flower anticancer
Efficacious prescriptions case.
The content of the invention
The technical problem to be solved in the present invention is to overcome the defect not on the effective scheme using crocodile mouth flower anticancer, is carried
Detected for a kind of Rh blood group antigens and blocked.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
A kind of crocodile mouth spends anticancer liquid, is prepared using following steps：
1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；
2), distilled water is cleaned 2 times, is freezed, and is ground into the fragment of 10~20mm, and 50 DEG C are incubated 1~2 hour；
3) distilled water of 100~120 times of fresh leaf fragment volume, is added, the compound of 0.02~0.05 times of leaf quality is added
Enzyme preparation is digested；
4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, adds linalool dense to end
Degree one thousandth；
5), 4~8 DEG C of preservations.
Further, the step 2) in when freezing, first -10~-20 DEG C of precoolings 3~6 hours, then -20 DEG C~-30
DEG C freezing 6~8 hours, then -30~-60 DEG C freeze 6~12 hours.Freeze by precooling and step by step, can be with maximum limit
The reservation crocodile mouth of degree spends the active ingredient of fresh blade；
Further crushing, the low-temperature grinding under conditions of -16 DEG C~-10 DEG C；By freezing and low-temperature grinding, can be with
Retain the active ingredient that crocodile mouth spends fresh blade more than 89%.
Further, the step 3) in complex enzyme formulation by peroxidase, protease, urase, laccase and acidity
Amylase is constituted.Preferably, complex enzyme formulation includes each component of following mass fraction：
1~5 part of peroxidase, proteinase 8~12 part, 4~10 parts of urase, 4~6 parts of laccase and acid starch enzyme composition
6~8 parts.Preferably, the step 3) in enzymolysis time be 10~20min.By processing with enzyme preparation, the cancer resistance that crocodile mouth is spent
Maximum can be performed to.
Crocodile mouth of the invention spends anticancer liquid, and preparation method is simple, the suppression to cancer cells such as liver cancer, stomach cancer, breast cancer, lung cancer
Rate processed reaches more than 50%；Long-term taking is not needed, various cardiovascular and cerebrovascular diseases, hypertension, high fat of blood, ephrosis can be effectively controlled
Etc. various complication, the microcirculation of conditioning each organ of human body comprehensively improves metabolic function, and nontoxic pair of effect can long-term taking, peace
Full property reliability.
Specific embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
A kind of crocodile mouth spends anticancer liquid, it is characterised in that prepared using following steps：
1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；
2), distilled water is cleaned 2 times, first -10 DEG C of precoolings 3 hours, and then -20 DEG C freeze 8 hours, then -60 DEG C of freezings 6
Hour；Into the fragment of 20mm, 50 DEG C are incubated 2 hours to low-temperature grinding under conditions of -10 DEG C；
3) distilled water of 100 times of fresh leaf fragment volume, is added, adding the complex enzyme formulation of 0.02 times of leaf quality carries out enzyme
Solution；The complex enzyme formulation includes each component of following mass fraction：1 part of peroxidase, 12 parts of protease, 4 parts of urase, paint
4 parts of enzyme and acid starch enzyme constitute 8 parts；Enzymolysis time is 20min；
4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, adds linalool dense to end
Degree one thousandth；
5), 4~8 DEG C of preservations.
Embodiment 2
A kind of crocodile mouth spends anticancer liquid, it is characterised in that prepared using following steps：
1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；
2), distilled water is cleaned 2 times, first -20 DEG C of precoolings 6 hours, and then -30 DEG C freeze 6 hours, then -30 DEG C of freezings
12 hours；Into the fragment of 10mm, 50 DEG C are incubated 1 hour to low-temperature grinding under conditions of -16 DEG C；
3) distilled water of 120 times of fresh leaf fragment volume, is added, adding the complex enzyme formulation of 0.05 times of leaf quality carries out enzyme
Solution；The complex enzyme formulation includes each component of following mass fraction：5 parts of peroxidase, proteinase 8 part, 10 parts of urase, paint
6 parts of enzyme and acid starch enzyme constitute 6 parts；Enzymolysis time is 10min；
4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, adds linalool dense to end
Degree one thousandth；
5), 4~8 DEG C of preservations.
Embodiment 3
A kind of crocodile mouth spends anticancer liquid, it is characterised in that prepared using following steps：
1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；
2), distilled water is cleaned 2 times, first -15 DEG C of precoolings 5 hours, and then -25 DEG C freeze 7 hours, then -45 DEG C of freezings
10 hours；Into the fragment of 15mm, 50 DEG C are incubated 1 hour to low-temperature grinding under conditions of -13 DEG C；
3) distilled water of 110 times of fresh leaf fragment volume, is added, adding the complex enzyme formulation of 0.03 times of leaf quality carries out enzyme
Solution；The complex enzyme formulation includes each component of following mass fraction：3 parts of peroxidase, 10 parts of protease, 7 parts of urase, paint
5 parts of enzyme and acid starch enzyme constitute 7 parts；Enzymolysis time is 15min；
4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, adds linalool dense to end
Degree one thousandth；
5), 4~8 DEG C of preservations.
Clinical trial
Proliferation experiment：
It is Cell Counting Kit-8 kits, has the dehydrogenase can be by the mitochondria in living cells
The WST-8 oxidation orange-yellow materials of production are dissolved in tissue culture medium (TCM), and it is deeper that cell breeds more fast then color.Cell propagation is real
Test result to show, crocodile mouth flower anticancer liquid of the invention can play HCC 7402 good mechanism work in culture medium in vitro
With, inhibiting rate reach 50% with.
The apoptosis experiment of cell
From Annexin V-FITC cell apoptosis detection kits.Annexin V selective binding phosphatidyl serines
(phosphatidylserine, abbreviation PS), the Annexin V marked with the fluorescence probe FITC with green fluorescence, i.e.,
Annexin V-FITC, it is possible to phosphatidyl silk ammonia simply and is directly detected very much with flow cytometer or fluorescence microscope
The key character of this Apoptosis that turns up of acid.The kit additionally provides propidium iodide stain liquid, and propidium iodide can contaminate
Color non-viable non-apoptotic cell or apoptosis late period lose the cell of cell membrane integrity, and red fluorescence is presented.For non-viable non-apoptotic cell, due to cell
The integrality of film has been lost, and Annexin V-FITC can be entered into cytoplasm, with the phosphatidyl on the inside of cell membrane
Serine is combined, so as to also make non-viable non-apoptotic cell that green fluorescence is presented.Our results of study to Apoptosis show that one is of the invention
Crocodile mouth flower anticancer liquid can be to playing very big lethal effect to cancer cell, flow cytomery result shows absolutely after 24 hours
Most cells are dead.
Internal anticancer experiment：
Bel7402 liver cancer tissues after fluorescent protein labeling are planted in the liver region of nude mice, mouse is entered after surviving
Row E Zui flower anticancer liquid gavage treatment of the invention, somatoscopy was carried out every 5 days to mouse tumor.Result shows that E Zui spends
The tumor suppression situation of anticancer liquid treatment group is substantially better than negative control group and low concentration treatment group.This preliminary E Zui flower that disclose is carried
Take thing inhibited for internal live tumor tissue.
The result of study of liver protection effect in male white rat body：
Due to finding that crocodile mouth flower anticancer liquid has the effect for promoting cancer cell multiplication in cell proliferation experiment in vitro, therefore
It is contemplated that whether E Zui contains the composition for promoting liver cell reparation and regeneration in spending.In order to verify the viewpoint we to great Bai
Mouse has carried out alcohol damaged nursing and has tested.Result finds, different degrees of in the rat liver of 80% white wine nursing 2 months to go out
Fat drop is showed, and the white mouse liver section that Hubei Province mouth pollen end is fed has been added in white wine substantially without Pathologic changes.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, it still may be used
Modified with to the technical scheme described in foregoing embodiments, or equivalent is carried out to which part technical characteristic.
All any modification, equivalent substitution and improvements within the spirit and principles in the present invention, made etc., should be included in of the invention
Within protection domain.
Claims (6)
1. a kind of crocodile mouth spends anticancer liquid, it is characterised in that prepared using following steps：
1), pluck crocodile mouth and spend fresh blade, cleaned up with clear water；
2), distilled water is cleaned 2 times, is freezed, and is ground into the fragment of 10~20mm, and 50 DEG C are incubated 1~2 hour；
3) distilled water of 100~120 times of fresh leaf fragment volume, is added, the complex enzyme system of 0.02~0.05 times of leaf quality is added
Agent is digested；
4) EDTA to final concentration 0.5mmol/L, is added, glycerine to final concentration 0.1g/L is added, linalool to final concentration thousand is added
/ mono-；
5), 4~8 DEG C of preservations.
2. crocodile mouth as claimed in claim 1 flower anticancer liquid, it is characterised in that the step 2) in when freezing, first -10~-20
DEG C precooling 3~6 hours, then -20 DEG C~-30 DEG C freeze 6~8 hours, and then -30~-60 DEG C freeze 6~12 hours.
3. crocodile mouth as claimed in claim 1 flower anticancer liquid, it is characterised in that the step 2) in crushing, -16 DEG C~-
Low-temperature grinding under conditions of 10 DEG C.
4. crocodile mouth as claimed in claim 1 flower anticancer liquid, it is characterised in that the step 3) in complex enzyme formulation by peroxide
Compound enzyme, protease, urase, laccase and acid starch enzyme composition.
5. crocodile mouth as claimed in claim 4 flower anticancer liquid, it is characterised in that the step 3) in complex enzyme formulation include with
The each component of lower mass fraction：
1~5 part of peroxidase, proteinase 8~12 part, 4~10 parts of urase, 4~6 parts of laccase and acid starch enzyme composition 6~8
Part.
6. crocodile mouth as claimed in claim 4 flower anticancer liquid, it is characterised in that the step 3) in enzymolysis time be 10~
20min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710035397.1A CN106727775A (en) | 2017-01-18 | 2017-01-18 | A kind of crocodile mouth spends anticancer liquid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710035397.1A CN106727775A (en) | 2017-01-18 | 2017-01-18 | A kind of crocodile mouth spends anticancer liquid |
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| Publication Number | Publication Date |
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| CN106727775A true CN106727775A (en) | 2017-05-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710035397.1A Pending CN106727775A (en) | 2017-01-18 | 2017-01-18 | A kind of crocodile mouth spends anticancer liquid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107648289A (en) * | 2017-10-18 | 2018-02-02 | 海南师范大学 | Sorrow escape careless extract preparation and its application in anti-tumor aspect |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103877133A (en) * | 2014-03-19 | 2014-06-25 | 江苏大学 | Extracting method of clinacanthus nutans anti-cancer active part and application thereof |
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN106190739A (en) * | 2016-07-22 | 2016-12-07 | 许备占 | Wild Hibisci Mutabilis dinner party with singsong girls in attendance |
-
2017
- 2017-01-18 CN CN201710035397.1A patent/CN106727775A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103877133A (en) * | 2014-03-19 | 2014-06-25 | 江苏大学 | Extracting method of clinacanthus nutans anti-cancer active part and application thereof |
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN106190739A (en) * | 2016-07-22 | 2016-12-07 | 许备占 | Wild Hibisci Mutabilis dinner party with singsong girls in attendance |
Non-Patent Citations (3)
| Title |
|---|
| LENG WEI KHOOA: "Phytochemical diversity of Clinacanthus nutans extracts and their bioactivity correlations elucidated by NMR based metabolomics", 《PHYTOCHEMISTRY LETTERS》 * |
| 张静: "《中药鉴定技术》", 29 February 2016 * |
| 陈长洲: "《微米中药——中药超细粉体的研究及应用》", 31 August 2007 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107648289A (en) * | 2017-10-18 | 2018-02-02 | 海南师范大学 | Sorrow escape careless extract preparation and its application in anti-tumor aspect |
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Application publication date: 20170531 |