CN106727750A - A kind of Suaeda salsa seed Extraction of carotenoid pigment technique - Google Patents
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique Download PDFInfo
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- CN106727750A CN106727750A CN201611040492.2A CN201611040492A CN106727750A CN 106727750 A CN106727750 A CN 106727750A CN 201611040492 A CN201611040492 A CN 201611040492A CN 106727750 A CN106727750 A CN 106727750A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention discloses a kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprise the following steps：（1）Suaeda salsa seed is fully ground under liquid nitrogen；（2）It is 3 to use volume ratio：1~1：4 petroleum ether and the mixed solvent of acetone as digestion agent, by digestion agent and step（1）Sample mixing after middle grinding, makes solid-liquid ratio be 1：（45~80）；（3）By step（2）In mixed solution carry out ultrasonic wave extraction, the ultrasonic extraction time is 8 ~ 18 min, and ultrasonic extraction number of times is 2 ~ 3 times, merges extract solution, and centrifugation is taken supernatant and dried up using nitrogen, obtains final product Suaeda salsa seed carotenoid.The present invention has filled up the blank of Extraction of carotenoid pigment method in Suaeda salsa seed, and screening and optimizing obtains a kind of preferably effective extracting method, extracts carotenoid for the deep processing of Suaeda salsa seed and recycling and scale and provide experiment basis.
Description
Technical field
The present invention relates to technical field of plant extraction, and in particular to a kind of Suaeda salsa seed Extraction of carotenoid pigment technique.
Background technology
In global range, the salination of soil and secondary salinization phenomenon generally existing.China is to possess saline and alkaline in the world
One of more country in ground, the salination phenomenon of coastal area is particularly acute.Halophytes can be normal in salination environment
Grow and complete the history of life, necessarily there is its specific salt tolerant mode.Suaeda salsa is that one kind is distributed widely in the Yellow River triangle
Continent area the annual halophytes of typical dilute salt form, its dimorphism seed seed color, Germination characteristics with
And salt tolerant aspect shows great difference, and the color of dimorphism seed is mainly determined by kind of a skin, therefore plants pigment product in skin
Tire out in terms of adaptation of the Suaeda salsa to environment and to having great importance in terms of the protection of seed.
Chromatic colour all is presented containing certain carotenoid in flower, fruit, the seed of plant, so as to attract to move
Thing propagates pollen, spore or seed, promotes the breeding of plant.Carotenoid is also applied in aquaculture, and chicken feed addition class is recklessly
The feed of radish element can deepen egg yellow, improve the nutritive value and healthcare function of yolk.In photosynthesis, class is recklessly
Radish element participates in light capture and transmits as accessory pigments, and participates in light protection and resistance oxidative damage (Liebler, 1993).
Suaeda salsa seed content has substantial amounts of carotenoid, its seed adapt to environment and to the protection of seed in terms of have it is important
Effect.Additionally, carotenoid can suppress, remove the free radical produced in body metabolic process, the work of anti-aging is played
With.Therefore, efficiently extracting the carotenoid of vegetable seeds has potential healthy benefits and economic worth.
Carotenoid generally exists in the form of carboxylate, therefore it isolates and purifies and usually needs saponification, and saponification can
To destroy chlorophyll, carotenoid mixture is set more to simplify.Carotenoid in alkaline environment can with stable existence, therefore one
As under room temperature or heating condition using potassium hydroxide methanol solution saponification (Britton, 1985).Chromatography often by with
In purifying carotenoid, thin layer chromatography (thin layer chromatography, TLC) is cheap, easy to be excellent because of its
Gesture and be widely used, high performance liquid chromatography (high-performance liquid chromatography, HPLC) can be right
Carotenoid does qualitative and quantitative analysis, have the advantages that sensitivity high, good resolution, it is quick (Biacs and Daood,
1994).However, but never being reported about the extracting method of carotenoid in Suaeda salsa seed.
The content of the invention
For prior art, the invention provides a kind of Suaeda salsa seed Extraction of carotenoid pigment technique, the extraction process
The carotenoid effect that method is extracted in Suaeda salsa seed is obvious, and method is effective.
The technical solution adopted by the present invention is as follows：
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
（1）Suaeda salsa seed is fully ground under liquid nitrogen；
（2）It is 3 to use volume ratio：1~1：4 petroleum ether and the mixed solvent of acetone as digestion agent, by digestion agent and step
（1）Sample mixing after middle grinding, makes solid-liquid ratio be 1g：（45~80）mL；
（3）By step（2）In mixed solution carry out ultrasonic wave extraction, each ultrasonic extraction time is 8 ~ 18min, and ultrasound is carried
Number of times is taken for 2 ~ 3 times, merges extract solution, centrifugation is taken supernatant and dried up using nitrogen, obtains final product Suaeda salsa seed carotenoid.
Step（1）In, there is vital influence on extraction efficiency on the pretreatment process of specific extraction object, this will
Determine the size of last recovery rate.Using liquid nitrogen grinding, can fully by Suaeda salsa seed grind into powder preferably will
Extraction of carotenoid pigment in material is out.Compare by same method for distinguishing, the method extraction efficiency highest.
Step（2）In, different digestion agents have different influences to extracting for carotenoid, and the present invention is for saline land
The fluffy seed of alkali this extracting object, screening and optimizing has obtained petroleum ether and acetone as Suaeda salsa from substantial amounts of organic solvent
The digestion agent of seed, the extracting effect using this digestion agent is excellent.By lot of experiment validation and analysis, the petroleum ether and
The volume ratio of acetone is 3：1~1：When 4, extracting effect is preferable, it is further preferred that the ratio between both volumes are 2：1~1：2, most
Preferably, the ratio between both volumes are 1：1.
The solid-liquid ratio is the ratio between volume of sample quality and the digestion agent after grinding, and solid-liquid ratio of the invention is to extracting
The content of carotenoids has material impact, by substantial amounts of experimental verification and analysis, solid-liquid ratio is arranged on into 1 g：（45~80）
During mL, the recovery rate of carotenoids is higher；Experiment proves to be more than 1 when solid-liquid ratio：45 and less than 1：When 80, Extraction of carotenoid pigment rate
It is significant lower.It is further preferred that the solid-liquid ratio is 1：45~60.Most preferably, the solid-liquid ratio is 1：When 50, carotenoids
The recovery rate of element is optimal.
Step（3）In, the extraction time of the ultrasonic wave is 2 times, and each extraction time is 8 ~ 10 min, most preferably
10 min.Go out from the experimental results, less than 2 times, the recovery rate of carotenoid is substantially reduced ultrasonic number of times, with number of times
Increase, the recovery rate of carotenoid also decreases.In theory, extraction time is more, the carotenoid dissolution in sample
More, recovery rate is got over and levels off to a certain steady state value, but in the present invention Extraction of carotenoid pigment rate with extraction time continue increase anti-
And reduce, extraction time reaches highest recovery rate for 2 times, and recovery rate does not increase with the increase of extraction time, and this is main
Be due in experimentation operation cause because extraction time is more, transfer, merge etc. operation increase, extract loss also compared with
Greatly, while extraction time is also corresponding long, therefore 2 i.e. available Extraction of carotenoid pigment rates higher of ultrasonic extraction of the present invention.
Supersonic frequency during ultrasonic extraction is 550 ~ 650 W, and ultrasonic temperature is 30 ~ 35 DEG C.Preferably, supersonic frequency is
600 W, ultrasonic temperature is 30 DEG C.Extraction of the ultrasonic power to Suaeda salsa carotenoid has large effect, through excessive
The experimental verification of amount, the supersonic frequency that the present invention chooses is 550 ~ 650 W, enables to the recovery rate of carotenoid higher, if
When being that ultrasonic power is less than 550 W, the recovery rate of carotenoid is relatively low；If be greater than 650 W, because active force is larger, instead
And carotenoid is decomposed, it is relatively low so as to cause recovery rate.
The present invention also protects the Suaeda salsa seed carotenoid that described technique is prepared.
Above-mentioned technical proposal has the advantages that：
（1）Suaeda salsa is being capable of the heavy salinized aerial that can not be grown in crops and a kind of salt that can complete the history of life
Plant, while salt-soda soil can be improved by absorbing substantial amounts of salinity, is referred to as " pionner " in salt-soda soil, and its
Hip number is big, if it is possible to a large amount of from Suaeda salsa seed to extract carotenoid, for grocery trade and industrial production, salt
Undoubtedly one kind is good material to the fluffy seed of ground alkali, it is also possible to reach more preferably improvement and the purpose using salt-soda soil.
At present, do not have the report of correlation on Extraction of carotenoid pigment in Suaeda salsa seed, and proposition of the invention,
Fill up the blank of Extraction of carotenoid pigment method in Suaeda salsa seed, and screening and optimizing obtains a kind of preferably effectively carrying
Method is taken, extracting carotenoid for the deep processing of Suaeda salsa seed and recycling and scale provides experiment basis.Fortune
The carotenoid in Suaeda salsa seed is extracted with the method, convenient material drawing, flow is simple, and strong operability, cost is relatively low, should
Extracting method is the most simple and easy to do, and recovery rate is higher, and up to 92.7%, extraction yield is 194.47 mg kg-1 DW。
（2）Compared with the nutrition organs of plant, the pigment in Suaeda salsa seed can typically be formed Suaeda salsa seed
A kind of more stable structure of matter（Play a part of protection vegetable seeds until sprouting）, therefore want from Suaeda salsa seed
Middle extraction pigment, it is necessary to fully crush seed material, farthest this pigment separated out, present invention employs liquid nitrogen
Under the conditions of the method that is fully ground, this can extract the key technology hand of carotenoid for the present invention from Suaeda salsa seed
Section.In addition, in extraction process, the technological parameter of the extraction of raw material is also extremely important.The present invention by screening and optimizing obtained compared with
The good technique for extracting carotenoid in Suaeda salsa seed, the recovery rate that the technique extracts carotenoid is high.
（3）The present invention chooses Suaeda salsa seed as raw material, and research obtains a kind of rapidly and effectively carotenoid and carries
Take method, for inquire into seed in adaptation sexual intercourse of the carotenoid content with Suaeda salsa seed to environment lay the foundation, also for
In industry, carotenoid is offered reference during grocery trade extracts Suaeda salsa seed on a large scale, and in research Suaeda salsa seed
The function of carotenoid, using and ecological significance lay the foundation.
Brief description of the drawings
Fig. 1：The ultrasonic extraction method technique of Suaeda salsa seed carotenoid, A：Solid-liquid ratio；B：Petroleum ether/acetone body
Product ratio；C：Extraction time；D：Extraction time.
Fig. 2：The ultraviolet-visible spectrophotometer spectrum of Suaeda salsa seed Extraction of carotenoid pigment liquid.
Specific embodiment
With reference to embodiment, the present invention is further described.
Embodiment 1
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
1）The g of Suaeda salsa seed 0.1 is weighed respectively, in mortar, is fully ground under liquid nitrogen.
2）The ratio for determining digestion agent is petroleum ether：Acetone=1:1.
3）Add 5 mL digestion agent (petroleum ethers：Acetone volume ratio=2:1) solid-liquid ratio, is made to be 1：50.
4）Each min of ultrasonic wave extraction 10, supersonic frequency is 600 W, and ultrasonic temperature is 30 DEG C.
5）Ultrasonic wave extraction 2 times, it is 1 that each ultrasonic extraction controls solid-liquid ratio：50（After first time ultrasonic wave extraction, mistake
Filter, the residue that will be obtained carries out second ultrasonic wave extraction）.
6）Merge extract solution, 5000 rpm are centrifuged 5 min, take supernatant and dried up with nitrogen, that is, obtain Suaeda salsa kind
Subclass carrotene.
Embodiment 2
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
1）The g of Suaeda salsa seed 0.1 is weighed respectively, in mortar, is fully ground under liquid nitrogen.
2）The ratio for determining digestion agent is petroleum ether：Acetone=1:1.
3）It is separately added into 2,3,5 and 10 mL digestion agent (petroleum ethers：Acetone volume ratio=1:1) solid-liquid ratio, is made to be 1:
20、1：30、1：50、1：100.
4）Each min of ultrasonic wave extraction 8, supersonic frequency is 580 W, and ultrasonic temperature is 35 DEG C.
5）Ultrasonic wave extraction 2 times, it is 1 that each ultrasonic extraction controls solid-liquid ratio：50.
6）Merge extract solution, 5000 rpm are centrifuged 5 min, take supernatant and dried up with nitrogen, that is, obtain Suaeda salsa kind
Subclass carrotene.Then 3 mL are settled to acetone, light absorbs is determined at 445 nm.
The carotenoid content in Suaeda salsa seed is extracted to shown in A in such as Fig. 1 by controlling solid-liquid ratio.
Embodiment 3
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
1）The g of Suaeda salsa seed 0.1 is weighed respectively, in mortar, is fully ground under liquid nitrogen.
2）The ratio for determining digestion agent is petroleum ether：Acetone=3：1、2:1、1：1、1：2 and 1：4.
3）It is separately added into 5 mL digestion agent (petroleum ethers：The score of acetone volume Wei 3：1、2:1、1：1、1：2 and 1：4),
Solid-liquid ratio is set to be 1：50.
4）Each min of ultrasonic wave extraction 8, supersonic frequency is 580 W, and ultrasonic temperature is 30 DEG C.
5）Ultrasonic wave extraction 2 times, it is 1 that each ultrasonic extraction controls solid-liquid ratio：50.
6）Merge extract solution, 5000 rpm are centrifuged 5 min, take supernatant and dried up with nitrogen, that is, obtain Suaeda salsa kind
Subclass carrotene.Then 3 mL are settled to acetone, light absorbs is determined at 445 nm.
The carotenoid content in Suaeda salsa seed is extracted to such as scheming by controlling the volume ratio of petroleum ether and acetone
In 1 shown in B.
Embodiment 4
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
1）The g of Suaeda salsa seed 0.1 is weighed respectively, in mortar, is fully ground under liquid nitrogen.
2）The ratio for determining digestion agent is petroleum ether：Acetone=1.5:1.
3）Add 5 mL digestion agent (petroleum ethers：Acetone volume ratio=1.5:1) solid-liquid ratio, is made to be 1：50.
4）Each ultrasonic wave extraction 2,5,10,20 and 30 min respectively, supersonic frequency is 620 W, and ultrasonic temperature is 30
℃。
5）Ultrasonic wave extraction 2 times, it is 1 that each ultrasonic extraction controls solid-liquid ratio：50.
6）Merge extract solution, 5000 rpm are centrifuged 5 min, take supernatant and dried up with nitrogen, that is, obtain Suaeda salsa kind
Subclass carrotene.Then 3 mL are settled to acetone, light absorbs is determined at 445 nm.
The carotenoid content in Suaeda salsa seed is extracted to shown in C in such as Fig. 1 by controlling extraction time.
Embodiment 5
A kind of Suaeda salsa seed Extraction of carotenoid pigment technique, comprises the following steps：
1）3 parts of g of Suaeda salsa seed 0.1 are weighed respectively, in mortar, are fully ground under liquid nitrogen.
2）The ratio for determining digestion agent is petroleum ether：Acetone=1.5:1.
3）Add 5 mL digestion agent (petroleum ethers：Acetone volume ratio=1.5:1) solid-liquid ratio, is made to be 1：50.
4）Each min of ultrasonic wave extraction 8, supersonic frequency is 620 W, and ultrasonic temperature is 30 DEG C.
5）Difference ultrasonic wave extraction 1,2 and 3 times, it is 1 that each ultrasonic extraction controls solid-liquid ratio：50.
6）Merge extract solution, 5000 rpm are centrifuged 5 min, take supernatant and dried up with nitrogen, that is, obtain Suaeda salsa kind
Subclass carrotene.Then 3 mL are settled to acetone, light absorbs is determined at 445 nm.
The carotenoid content in Suaeda salsa seed is extracted to D institutes in such as Fig. 1 by controlling ultrasonic extraction number of times
Show.
Based on the carotenoid for extracting it is more stable on the premise of, present invention determine that carrying at utmost, rapidly and efficiently
The preferred plan for taking carotenoid in Suaeda salsa seed is（Described in embodiment 1）：The g of Suaeda salsa seed 0.1, digestion agent
Ratio is petroleum ether：Acetone=1:1, solid-liquid ratio is 1:50, the min of ultrasonic wave extraction 10, extract 2 times, 5000 rpm centrifugations 5
Min, takes supernatant and is dried up with nitrogen.
(Fig. 1) is contrasted by extracting the carotenoid content in Suaeda salsa seed with different process method, can be seen
Go out, the g of Suaeda salsa seed 0.1, the ratio of digestion agent is petroleum ether：Acetone=1:1, solid-liquid ratio is 1:50, each ultrasonic wave is carried
10 min are taken, is extracted 2 times, 5000 rpm are centrifuged 5 min, taken supernatant and dried up with nitrogen, 3 mL, 445 are settled to acetone
Light absorbs are determined at nm（Fig. 2）, the maximum amount of carotenoid can be obtained, recovery rate is 92.7%, and extraction yield is 194.47 mg
kg-1DW, extracts yield（mg kg-1DW）The extracted amount of carotenoid in=optimised process（mg）The dry weight of/material（kg）.
As seen from Figure 1, the carotenoid effect extracted in Suaeda salsa seed using the process for extracting is obvious, compared to
Other process difference on effect significantly, illustrate that the method is effective.
Above-described embodiment is the present invention preferably implementation method, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from Spirit Essence of the invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of Suaeda salsa seed Extraction of carotenoid pigment technique, it is characterized in that, comprise the following steps：
（1）Suaeda salsa seed is fully ground under liquid nitrogen；
（2）It is 3 to use volume ratio：1~1：4 petroleum ether and the mixed solvent of acetone as digestion agent, by digestion agent and step
（1）Sample mixing after middle grinding, makes solid-liquid ratio be 1g：（45~80）mL；
（3）By step（2）In mixed solution carry out ultrasonic wave extraction, ultrasonic power is 550 ~ 650 W, and ultrasonic temperature is 30
~ 35 DEG C, each ultrasonic extraction time is 8 ~ 15 min, and ultrasonic extraction number of times is 2 ~ 3 times, merges extract solution, and centrifugation takes supernatant
Liquid is dried up using nitrogen, obtains final product Suaeda salsa seed carotenoid.
2. extraction process as claimed in claim 1, it is characterized in that：Step（2）In, the digestion agent petrochina ether and acetone
Volume ratio is 2：1~1：2.
3. extraction process as claimed in claim 2, it is characterized in that：Step（2）In, the digestion agent petrochina ether and acetone
Volume ratio is 1：1.
4. extraction process as claimed in claim 1, it is characterized in that：Step（2）In, the solid-liquid ratio is 1 g：（45~60）
mL。
5. extraction process as claimed in claim 4, it is characterized in that：Step（2）In, the solid-liquid ratio is 1 g：50 mL.
6. extraction process as claimed in claim 1, it is characterized in that：Step（3）In, the extraction time is 2 times.
7. extraction process as claimed in claim 1, it is characterized in that：Step（3）In, each extraction time is 8 ~ 10
min。
8. extraction process as claimed in claim 7, it is characterized in that：Step（3）In, each extraction time is 10 min.
9. extraction process as claimed in claim 1, it is characterized in that：The g of Suaeda salsa seed 0.1, the volume of digestion agent
Ratio is petroleum ether：Acetone=1:1, solid-liquid ratio is 1:50, the min of each ultrasonic wave extraction 10, extract 2 times, 5000 rpm centrifugations 5
Min, takes supernatant and is dried up with nitrogen.
10. the Suaeda salsa seed carotenoid for being prepared using the technique any one of claim 1 ~ 9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659570A (en) * | 2018-05-14 | 2018-10-16 | 汕头大学 | A kind of modification method of the internal total carotinoid of extraction Chlamys nobilis " Nan'ao gold shellfish " |
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2016
- 2016-11-10 CN CN201611040492.2A patent/CN106727750B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
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| 张巍等: "超声波法提取锦灯笼色素及稳定性的研究", 《安徽农业科学》 * |
| 赵海林等: "盐地碱蓬食用价值的研究", 《安徽农业科学》 * |
| 高玉龙等: "新鲜烟叶不同处理对质体色素含量的影响", 《内蒙古农业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659570A (en) * | 2018-05-14 | 2018-10-16 | 汕头大学 | A kind of modification method of the internal total carotinoid of extraction Chlamys nobilis " Nan'ao gold shellfish " |
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