CN106727431A - TPGS modification carries tigecycline nano grain of silver and preparation and application - Google Patents

TPGS modification carries tigecycline nano grain of silver and preparation and application Download PDF

Info

Publication number
CN106727431A
CN106727431A CN201710047249.1A CN201710047249A CN106727431A CN 106727431 A CN106727431 A CN 106727431A CN 201710047249 A CN201710047249 A CN 201710047249A CN 106727431 A CN106727431 A CN 106727431A
Authority
CN
China
Prior art keywords
silver
tigecycline
nano grain
tpgs
modification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710047249.1A
Other languages
Chinese (zh)
Other versions
CN106727431B (en
Inventor
杜永忠
康旭琪
姜赛平
应晓英
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang University ZJU
Original Assignee
Zhejiang University ZJU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang University ZJU filed Critical Zhejiang University ZJU
Priority to CN201710047249.1A priority Critical patent/CN106727431B/en
Publication of CN106727431A publication Critical patent/CN106727431A/en
Application granted granted Critical
Publication of CN106727431B publication Critical patent/CN106727431B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/513—Organic macromolecular compounds; Dendrimers
    • A61K9/5146—Organic macromolecular compounds; Dendrimers obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/65—Tetracyclines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/38—Silver; Compounds thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Nanotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Inorganic Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Manufacture Of Metal Powder And Suspensions Thereof (AREA)
  • Medicinal Preparation (AREA)

Abstract

The present invention provides a kind of load tigecycline nano grain of silver of TPGS modification.First under the stabilization of TPGS, hollow nano grain of silver is formed by Kinkendal Effect, synthesizing polyethylene glycol VE-succinate modifies nano grain of silver, improves the stability of hollow nano grain of silver, reduces the toxicity caused by nano grain of silver oxidation.The hollow structure pay(useful) load antibacterials tigecycline of tigecycline nano grain of silver is carried using TPGS modification.The nanoparticle has less particle diameter, may pass through the cell membrane of bacterium, destruction cell membrane, into cytoplasm, and by nano grain of silver and the synergetic antibacterial effect of tigecycline, the minimal inhibitory concentration and MBC of medicine are significantly reduced, Escherichia coli are overcome to the drug resistance of tigecycline.Antibacterium medicine-resistant medicine can be prepared as novel antibacterial nano material, be applied to life science and pharmaceutical field.

Description

TPGS modification carries tigecycline nano grain of silver and preparation With application
Technical field
The invention belongs to the preparation of nanoparticle, it is related to a kind of load tigecycline nano grain of silver, and in particular to polyethylene glycol dimension life Plain E succinates modify nano grain of silver and preparation method, and TPGS modification nano grain of silver in system Application in standby drug-resistance bacteria medicine tigecycline.
Background technology
In recent years, as antibacterials are in clinical extensive use, multidrug resistant and general antibody-resistant bacterium are increasing, motionless Bacillus occurs in that the even full persister of multidrug resistant, causes Partial Hospitals to break out acinetobacter calcoaceticus prevalence, to the treatment of clinical infection Bring stern challenge.Tigecycline (Tigecycline) is as first (in June, 2005) by United States food and drag administration The Tigecycline class antibacterials of the wide spectrum of new generation of (Food and Drug Administration, FDA) approval, energy Overcome the tetracycline resistance caused by row outside bacterium and ribosomes protect, good is kept to common pathogen or multi-drug resistant bacteria Good antibacterial activity, " finally selecting " as the clinical multidrug resistant of control or general resistance acinetobacter, but also have research It was found that acinetobacter calcoaceticus there is also drug resistance to tigecycline, its resistance mechanism may actively outer row be relevant with extra-pumping system.
Nano material has excellent comprehensive at aspects such as mechanics, optics, magnetic, superconductivity, dielectric properties, optical properties Performance is closed, therefore has attracted the extensive concern of national governments and researcher.Nano silver grain is used as a kind of emerging function Nano material, with specific surface area and surface-active very high, in catalysis material, antistatic material, low temperature superconducting material and life The aspects such as thing sensor material are widely used.The anti-microbial property of nano grain of silver early has research, can kill be in contact with it most Number bacteriums, fungi, mould, the microorganism such as spore, and to pathogenic bacteria of drug-resistant such as antibiotic-resistance E. coli, resistance golden staphylococci, resistance to Medicine Pseudomonas aeruginosa, streptococcus pyogenes, resistance enterococcus, anaerobic bacteria etc. have comprehensive antibacterial activity, are led in anti-biotic material in recent years Domain is widely used.Generally believe that the sterilization idiocratic of nano grain of silver may be carried out by following different approaches at present：(1) by with The protein molecule of bacterium surface is combined, and cracks the permeability of proton pump, regulation memebrane protein or phospholipid bilayer so that H+Outward Leakage, causes the cell membrane lysis of bacterium and plays bactericidal action；(2) suppress various thin with the electron donor reaction containing sulfydryl The activity of enzyme, plays antibacterial action on after birth；(3) with protein and nucleic acid reaction, the inhereditary material of bacterium is destroyed, suppresses to increase Grow；(4) nano grain of silver enters thalline and is combined with its inhereditary material, forms irreversible condensate, causes the change of DNA configurations Suppress its breeding.With going deep into that nano grain of silver is studied, its potential toxic and side effect is increasingly highlighted, such as internal accumulative, god Through toxicity and embryonic development toxicity etc..Nano grain of silver surface is oxidized easily, and ion is easily discharged in water, body fluid, organism State silver is the main cause of nano grain of silver toxic action.
TPGS (D- α-Tocopherol polyethylene glycol succinate, TPGS it is) a kind of safe, excellent nonionic surface active agent, is widely used in polymer nanoparticle, polymer micelle, receives In the nano medicament carrying systems such as Mizhi plastid.It has the structure of Renascin, there is certain oxidation resistance, possesses polyethylene glycol Advantage (internal long circulating, Increased Plasma Half-life, the cellular uptake for improving medicine), and with the outer row function of P glycoprotein is suppressed, can It is applied to suppress drug-resistant bacteria efflux pump, realization overcomes resistance.
The content of the invention
First purpose of the invention is to provide a kind of TPGS modification load tigecycline silver and receives The grain of rice, the nanoparticle is made up of TPGS, nano grain of silver and tigecycline, its mass percent For：TPGS 63.13%-94.51%；Nano grain of silver 1.82%-33.96%；Tigecycline 2.54%-3.84%.The molecular weight ranges of TPGS are 1513~10513, and structural formula is as follows：
Wherein the degree of polymerization (n) of polyglycol chain is 23~230.
Second object of the present invention is to provide TPGS modification and carries tigecycline nano grain of silver Preparation method, especially by following approach realize：
1. TPGS modifies the synthesis of nano grain of silver
Take 15.6mL deionized waters to be placed in 30mL cillin bottles, be positioned in ice-water bath Ultrasonic Pulverization instrument, first add 0.9mL Silver nitrate aqueous solution (10mM), be subsequently added the 120 μ L TPGSs aqueous solution (concentration be 10mM~ 80mM) ultrasonic mixing, adds sodium hydrate aqueous solution, and reaction solution quickly becomes faint yellow, and it is left that solution ph rises a height of 12 The right side, now forms silver oxide nanocrystal.It is freshly prepared to being rapidly added in prepared silver oxide nanocrystal solution 1.08mL sodium borohydride solutions (10mM), under continuation lucifuge ice-water bath after ultrasound 1h, product are placed in centrifuge tube 12000rpm is centrifuged 10min, removes supernatant, and collection is precipitated and is washed with deionized three times, and poly- second two is obtained after freezing Alcohol VE-succinate modifies nano grain of silver.
2. TPGS modification carries the preparation of tigecycline nano grain of silver
Precision weighs tigecycline 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL 10mL；In 5mL test tubes add 877mg TPGS modification nano grain of silver, then by for plus ring Element：The mass percent 20%~42.6% of TPGS modification nano grain of silver adds 0.5mg/mL's The tigecycline aqueous solution is appropriate, and being eventually adding the deionized water of certain volume makes final solution volume for 2mL, and 37 are placed in after mixing In DEG C constant temperature gas bath oscillator, after 100r/min concussions are incubated 24h, collect heavy by solution 12, after 000rpm high speed centrifugations 10min Form sediment, be precipitated after being washed with deionized 3 times and carry tigecycline silver nanoparticle as TPGS modification Grain.
Third object of the present invention is to provide a kind of TPGS modification load tigecycline silver and receives Application of the grain of rice in the antibacterium medicine-resistant medicine for carrying tigecycline is prepared.TPGS modification silver is received The hollow structure of the grain of rice can pay(useful) load antibacterials tigecycline, reach the slow release of medicine, using its small particle enter Bacterium realizes medicine delivery in bacterium, and can be tieed up by the synergetic antibacterial effect and polyethylene glycol of nano grain of silver and tigecycline The effect of the suppression efflux protein of raw element E succinates, improves the intake of bacteria medicine tigecycline, significantly reduces and replaces Plus the minimal inhibitory concentration and MBC of ring element, overcome the drug resistance of insensitive Escherichia coli.Described nano grain of silver Can be absorbed by sensitive Escherichia coli and insensitive Escherichia coli, penetration cell wall, into bacterial cytoplasm, it is in sensitive large intestine Minimal inhibitory concentration and MBC on bacillus are significantly reduced compared to the tigecycline aqueous solution, are shown stronger Synergetic antibacterial effect.Minimal inhibitory concentration and MBC on insensitive Escherichia coli are less than the tigecycline aqueous solution The modification of minimal inhibitory concentration and MBC on sensitive Escherichia coli, i.e. TPGS is carried Tigecycline nano grain of silver overcomes resistance completely on insensitive Escherichia coli, realizes and overcomes drug-fast bacteria resistance effect.
By the present invention in that being repaiied to hollow nano grain of silver as stabilizer with TPGS Decorations, the nano grain of silver of synthesizing stable low toxicity；Replaced plus ring further with the hollow structure pay(useful) load antibacterials of nano grain of silver Element prepares TPGS modification and carries tigecycline nano grain of silver；In polyethylene glycol tocopheryl succinate Under the modification of acid esters the nanoparticle can more stable safe performance synergetic antibacterial effect, significantly reduce the minimum antibacterial of medicine Concentration and MBC, realization overcome resistance.The TPGS modification for providing of the invention is carried for adding Ring element nano grain of silver, can be carried out efficiently by its hollow structure as the novel nano anti-biotic material for stablizing low toxicity to medicine Encapsulating and then Synergistic antimicrobial；Medicine delivery in bacterium is realized using its less particle diameter, the fungistatic effect of medicine is significantly improved, it is real Resistance now is overcome to drug-fast bacteria, life science and pharmaceutical field is can be widely applied to.
The present invention is modified hollow nano grain of silver surface by TPGS, is improved hollow The stability of nano grain of silver, reduces the toxicity caused by nano grain of silver oxidation；Using the structural load antibacterial of hollow nano grain of silver Medicine tigecycline；The nanoparticle has less particle diameter, may pass through bacterium cell membrane, destruction cell membrane, into cytoplasm, And by nano grain of silver and the synergetic antibacterial effect of tigecycline, minimal inhibitory concentration and the minimum sterilization for significantly reducing medicine are dense Degree, overcomes Escherichia coli to the drug resistance of tigecycline.
Brief description of the drawings
Fig. 1 is the transmission electron microscope observing result that TPGS modifies nano grain of silver.A is in figure Transmission electron microscope photo under 80KV accelerating potentials, B is high resolution TEM photo under 200KV accelerating potentials.
Fig. 2 is the drug release patterns in vitro that TPGS modification carries tigecycline nano grain of silver.
Fig. 3 is the bacterium intake section transmission that TPGS modification carries tigecycline nano grain of silver Electron microscopic observation result.
Specific embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
The modification of the TPGS of embodiment one carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (40mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, the rapid blackening of solution is subsequent It is changed into the yellow solution with kermesinus, under continuation lucifuge ice-water bath after ultrasound 1h, product is placed in 12 in centrifuge tube, 000rpm is centrifuged 10min, removes supernatant, and collection is precipitated and is washed with deionized three times, and polyethylene glycol is obtained after freezing VE-succinate modifies nano grain of silver.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 477nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.Observation result is as shown in figure 1, the particle size that circle can be observed is the nano grain of silver with hollow structure of 10nm or so Distribution.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.84 ± 0.86nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=28.5% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 3.84%.
The modification of the TPGS of embodiment two carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension (40mM, the ultrasonic mixing of the polyglycol chain degree of polymerization (n)=230), adds NaOH water-soluble for the raw element E succinates aqueous solution Liquid, reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To system Be rapidly added freshly prepared 1.08mL sodium borohydride solutions (10mM), the rapid blackening of solution in silver oxide nanocrystal solution The yellow solution with kermesinus is subsequently changed to, under continuation lucifuge ice-water bath after ultrasound 1h, product is placed in centrifuge tube 12,000rpm centrifugation 10min, remove supernatant, and collection is precipitated and is washed with deionized three times, and poly- second two is obtained after freezing Alcohol VE-succinate modifies nano grain of silver.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 477nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.Observation result is as shown in figure 1, the particle size that circle can be observed is the nano grain of silver with hollow structure of 10nm or so Distribution.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.84 ± 0.86nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=28.5% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 3.67%.
The modification of the TPGS of embodiment three carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (40mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 477nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.Observation result is as shown in Fig. 2 the particle size that circle can be observed is the nano grain of silver with hollow structure of 10nm or so Distribution.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.84 ± 0.86nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=20.0% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 2.71%.
The modification of example IV TPGS carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (40mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 477nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.Observation result is as shown in Fig. 2 the particle size that circle can be observed is the nano grain of silver with hollow structure of 10nm or so Distribution.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.84 ± 0.86nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=42.6% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 3.02%.
The modification of the TPGS of embodiment five carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (10mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 478nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.81 ± 0.59nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=20.0% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 2.54%.
The modification of the TPGS of embodiment six carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (10mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 478nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.81 ± 0.59nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=42.6% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 3.11%.
The modification of the TPGS of embodiment seven carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (80mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 481nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.93 ± 0.54nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=20.0% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 2.68%.
The modification of the TPGS of embodiment eight carries the preparation of tigecycline nano grain of silver
TPGS modification carries tigecycline nano grain of silver and is carried out in two steps synthesis, and the first step is closed Into TPGS modification nano grain of silver is obtained, take 15.6mL deionized waters and be placed in 30mL cillin bottles, put It is placed in ice-water bath Ultrasonic Pulverization instrument, first adds 0.9mL silver nitrate aqueous solutions (10mM), is subsequently added 120 μ L polyethylene glycol dimension The raw element E succinates aqueous solution (80mM, the ultrasonic mixing of polyglycol chain polymerization degree n=23), adds sodium hydrate aqueous solution, Reaction solution quickly becomes faint yellow, and solution ph rises a height of 12 or so, now forms silver oxide nanocrystal.To prepared oxygen Freshly prepared 1.08mL sodium borohydride solutions (10mM) are rapidly added in change silver nanoparticle crystalloid solution, are continued under lucifuge ice-water bath After ultrasonic 1h, product is placed in centrifuge tube 10min is centrifuged in 12,000rpm, remove supernatant, collection is precipitated and spent Ion water washing three times, obtains TPGS modification nano grain of silver after freezing.
Spectral quality point is carried out to TPGS modification nano grain of silver using ultraviolet specrophotometer Analysis.Taking polyethylene glycol VE-succinate modification nano grain of silver is scattered in deionized water, makes its concentration for 0.1mg/mL, Its ultraviolet spectra is scanned in the range of 300~800nm of wavelength by ultraviolet specrophotometer, according to the peak height and maximum of absworption peak The Ultraviolet Absorption Characteristics of the position analysis nano grain of silver of absworption peak.Result display gained TPGS is repaiied Decorations nano grain of silver maximal ultraviolet absorption peak, in 477nm, is hollow nano grain of silver.
Crystal structure confirmation is carried out to TPGS modification nano grain of silver using X-ray diffractometer. Take lyophilized TPGS modification nano grain of silver powder 0.5g.Recorded with X-ray diffractometer, scan model It is 2 θ=10 °~90 ° to enclose, and according to the position (2 θ) of x-ray diffraction line and its corresponding diffracted ray relative intensity (I/I0), look into The powder diffraction data card of known standard is looked for, Discriminating materials are carried out, crystal structure analysis is carried out to gained nano grain of silver.As a result Display gained TPGS modification nano grain of silver is the silver-colored crystal of face-centred cubic structure.
Take a certain amount of dry TPGS modification nano grain of silver powder and polyethylene glycol vitamin E succinate powders, mix and grind uniform with appropriate KI powder, and transparent sheet is pressed into by tablet press machine, use Fu Vertical leaf transformation infrared spectrometer carries out IR spectrum scanning analysis to sample, and sweep limits is 400~4000cm-1, it is red by its Outer absorption peak position and intensity carry out characteristic peak identification and Discriminating materials to sample.Result display gained polyethylene glycol vitamin E Succinate modification nano grain of silver surface confirmation has the coating of TPGS.
Apparent form sight is carried out to TPGS modification nano grain of silver using transmission electron microscope Examine.Weigh certain nano grain of silver and be scattered in deionized water the nanoparticle solution for obtaining that concentration is 0.13mg/mL, then by sample drop It is big by transmission electron microscope (80kV) observation nanoparticle form and particle diameter after drying at room temperature in being coated with the copper mesh of carbon film It is small.
Taking polyethylene glycol VE-succinate modification nano grain of silver solution is appropriate, is surveyed with particle size and surface potential Determine the particle diameter that instrument determines nanoparticle with surface potential detection instrument.After measured, TPGS modification silver nanoparticle The particle diameter of grain is 9.84 ± 0.86nm.
Second step prepares TPGS modification and carries tigecycline nano grain of silver, and precision is weighed for adding Ring element 5mg, is dissolved in deionized water and preparing the tigecycline aqueous solution of 0.5mg/mL；877mg is added in 5mL test tubes TPGS modification nano grain of silver, then by tigecycline：TPGS The dosage of modification nano grain of silver=42.6% (w/w) adds the tigecycline aqueous solution of 0.5mg/mL appropriate, is eventually adding one Determining the deionized water of volume makes final solution volume for 2mL, is placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, 100r/min shakes After swinging incubation 24h, by solution 12, precipitation is collected after 000rpm high speed centrifugations 10min, sunk after being washed with deionized 3 times Form sediment and be TPGS modification load tigecycline nano grain of silver.
Using the content of ultraviolet spectrometry spectrographic determination tigecycline.Standard curve：Precision weighs 1.0mg tigecycline marks Quasi- product, with deionized water dissolving constant volume in 25mL volumetric flasks, obtain final product the standard reserving solution of 40 μ g/mL.Accurate absorption should respectively It is 5,6,9,12,15,18,21,24 μ g/mL that a certain amount of deionized water constant volume of storing solution obtains concentration in 10mL volumetric flasks Tigecycline standard liquid, then take 1mL respectively its absorbance is determined at 245nm by ultraviolet specrophotometer, with extinction Degree carries out linear regression to tigecycline solution concentration, draws the ultraviolet standard curve of tigecycline.
The medicine that TPGS modification carries tigecycline nano grain of silver is determined using supercentrifugal process Drugloading rate.Take the modification of 2mL TPGSs and carry tigecycline nano grain of silver solution 12,000rpm at a high speed Supernatant is collected after centrifugation 10min, after dilution suitable multiple, is added using dissociating in ultraviolet spectrometry spectrographic determination supernatant Ring element drug concentration, obtains TPGS and modifies unentrapped in load tigecycline nano grain of silver solution The content of tigecycline, free tigecycline content is subtracted with the tigecycline content=total content of nanometer particle loading.Press Formula calculates drug delivery amount respectively.
Drugloading rate=(medication amount/drug-carrying nanometer particle total amount in nanoparticle) × 100%
After measured, the drugloading rate that TPGS modification carries tigecycline nano grain of silver is 3.09%.
The TPGS of embodiment nine modifies nano grain of silver in antibacterials tigecycline is prepared Application
1. TPGS modification carries the vitro drug release behavior of tigecycline nano grain of silver
The phosphate buffer for preparing pH 5.5 and pH 7.4 simulates bacterial infections in vivo position and normal portions respectively Physiological environment condition.Weigh the modification of 7.9g TPGSs and carry tigecycline nano grain of silver (embodiment one) It is scattered in the release pipe equipped with 12mL dissolution mediums, the release of medicine meets sink conditions, in 37 DEG C, 70rpm waters bath with thermostatic control Vitro drug release experiment is carried out under vibration, particular point in time (0.5h, l h, 2h, 4h, 6h, 8h, 10h, 24h, 36h and 48h) sample, take 1mL mixed solutions 12,0.5mL supernatants are taken out after 000rpm centrifugations 10min, add the fresh releases of 0.5mL Continue isothermal vibration in the resuspended rear add-back release pipe of medium, by the drug concentration (inspection in high effective liquid chromatography for measuring sample Survey wavelength is 245nm), calculate the cumulative release amount and total release percentage of medicine.With method, three groups of parallel groups are set.
After measured, TPGS modification carries release behavior such as Fig. 2 institutes of tigecycline nano grain of silver Show, TPGS modification load tigecycline nano grain of silver release behavior in two kinds of dissolution mediums is similar, To 48h, Cumulative release amount respectively reaches 89.31% He in the dissolution medium of pH5.5 and pH7.4 for sustainable release 88.99%.
2. TPGS modification carries the bacterium intake research of tigecycline nano grain of silver
Sensitive Escherichia coli and insensitive Escherichia coli are cultivated as model bacterium, by polyethylene glycol VE succinic acid Ester modification carries after tigecycline nano grain of silver (embodiment 1) is incubated 20h with 37 DEG C of exponential phase bacterial suspension 5000rpm at a high speed After centrifugation 15min takes washing of precipitate, fixation of bacteria overnight, prepares section transmission electron microscope sample in 2.5% glutaraldehyde solution, leads to Cross bacterial structure and nanoparticle intake situation in transmission electron microscope observation section.Result is as shown in figure 3, can be observed particle diameter It is 10nm or so, the distribution of the nano grain of silver of profile rounding in bacterial cytoplasm.
3. TPGS modification carries the antibacterial property research of tigecycline nano grain of silver
The sensitive Escherichia coli of culture and insensitive Escherichia coli give polyethylene glycol tocopheryl succinate as model bacterium Acid esters modification carries tigecycline nano grain of silver and tigecycline evaluates its bacteriostasis property.Experiment is divided into polyethylene glycol vitamin E amber The modification of amber acid esters carries tigecycline nano grain of silver group, TPGS modification nano grain of silver group and for ring is added Plain group, quantitative minimal inhibitory concentration is carried out to sensitive Escherichia coli and insensitive Escherichia coli using micro broth dilution method (MIC) and sxemiquantitative MBC (MBC) determine, its fungistatic effect is evaluated.Result is as shown in table 1, in sensitivity On Escherichia coli, TPGS modification carry tigecycline nano grain of silver group compared to tigecycline group and TPGS modifies nano grain of silver group, and its minimal inhibitory concentration and MBC are significantly reduced, and assists With performance antibacterial action so that antibacterials tigecycline can more preferably play drug effect；On insensitive Escherichia coli, polyethylene glycol VE-succinate modification carries the minimal inhibitory concentration and MBC of tigecycline nano grain of silver group less than for ring is added The plain group of minimal inhibitory concentration and MBC in sensitive Escherichia coli, that is, reached and overcome insensitive large intestine bar completely Bacterium resistance effect.
Table 1：TPGS modification carries tigecycline nano grain of silver, polyethylene glycol tocopheryl succinate Acid esters modifies the MBC and minimal inhibitory concentration of nano grain of silver and tigecycline

Claims (4)

1. a kind of load tigecycline nano grain of silver that TPGS is modified, it is characterised in that the nanoparticle It is made up of TPGS, nano grain of silver and tigecycline, its mass percent is：Polyethylene glycol is tieed up Raw element E succinates 63.13%-94.51%；Nano grain of silver 1.82%-33.96%；Tigecycline 2.54%-3.84%, gathers The molecular weight ranges of ethylene glycol VE-succinate are 1513~10513, and structural formula is as follows：
Wherein polymerization degree n=23~230 of polyglycol chain.
2. a kind of TPGS modification according to claim 1 carries tigecycline nano grain of silver, its It is characterised by, tigecycline therein is sustainable in two kinds of dissolution mediums of pH5.5 and pH7.4 to be discharged to 48h.
3. a kind of preparation of the load tigecycline nano grain of silver of TPGS modification described in claim 1 Method, it is characterised in that realized by following steps：
(1) TPGS modifies the synthesis of nano grain of silver
Remove ionized water to be placed in cillin bottle, be positioned in ice-water bath Ultrasonic Pulverization instrument, first add silver nitrate aqueous solution, then add Enter the TPGS aqueous solution that concentration is 10mM~80mM, ultrasonic mixing adds NaOH water-soluble Liquid, reaction solution quickly becomes faint yellow, and solution ph rises a height of 12, now silver oxide nanocrystal is formed, to prepared oxygen Freshly prepared sodium borohydride solution is rapidly added in change silver nanoparticle crystalloid solution, under continuation lucifuge ice-water bath after ultrasound, will be anti- Answer product to be placed in centrifuge tube and 10min is centrifuged in 12000rpm, remove supernatant, collect and precipitate and be washed with deionized three It is secondary, TPGS modification nano grain of silver is obtained after freezing；
(2) TPGS modification carries the preparation of tigecycline nano grain of silver
Precision weighs tigecycline, be dissolved in deionized water prepare by the tigecycline aqueous solution, added in test tube poly- Ethylene glycol VE-succinate modifies nano grain of silver, then by tigecycline：TPGS is modified The mass percent 20%~42.6% of nano grain of silver adds the tigecycline aqueous solution of 0.5mg/mL, is eventually adding deionized water Make final solution volume for 2mL, be placed in after mixing in 37 DEG C of constant temperature gas bath oscillators, after 100r/min concussions are incubated 24h, will be molten Liquid 12, collects precipitation after 000rpm high speed centrifugations, be precipitated after being washed with deionized 3 times as polyethylene glycol vitamin E Succinate modification carries tigecycline nano grain of silver.
4. a kind of TPGS modification described in claim 1 carries tigecycline nano grain of silver and is preparing Carry the application in the antibacterium medicine-resistant medicine of tigecycline, it is characterised in that significantly reduce the minimal inhibitory concentration and most of medicine Small bacteriocidal concentration.
CN201710047249.1A 2017-01-22 2017-01-22 Polyethylene glycol vitamin E succinate modification carries tigecycline nano grain of silver and preparation and application Active CN106727431B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710047249.1A CN106727431B (en) 2017-01-22 2017-01-22 Polyethylene glycol vitamin E succinate modification carries tigecycline nano grain of silver and preparation and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710047249.1A CN106727431B (en) 2017-01-22 2017-01-22 Polyethylene glycol vitamin E succinate modification carries tigecycline nano grain of silver and preparation and application

Publications (2)

Publication Number Publication Date
CN106727431A true CN106727431A (en) 2017-05-31
CN106727431B CN106727431B (en) 2019-10-29

Family

ID=58944006

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710047249.1A Active CN106727431B (en) 2017-01-22 2017-01-22 Polyethylene glycol vitamin E succinate modification carries tigecycline nano grain of silver and preparation and application

Country Status (1)

Country Link
CN (1) CN106727431B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109908175A (en) * 2019-03-21 2019-06-21 华南农业大学 A kind of organic/inorganic joint bactericidal composition and the preparation method and application thereof
CN112336858A (en) * 2020-10-09 2021-02-09 华南理工大学 Bismuth-manganese-based composite particle and preparation method and application thereof
CN113304122A (en) * 2021-06-02 2021-08-27 浙江大学 Ts antibacterial peptide-TPGS modified composite nano drug delivery system and preparation and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101880381A (en) * 2010-06-30 2010-11-10 清华大学深圳研究生院 Segmented copolymer modified by polyethylene glycol 1000 vitamin E succinic acid ester, preparation method and applications thereof
CN104368010A (en) * 2014-11-26 2015-02-25 苏州纳思达生物医药有限公司 Application of vitamin E polyethylene glycol succinate and derivatives thereof in preparation of hydrogel nanoparticle preparation of prodrug of hydrophilic medicine
CN104473965A (en) * 2005-08-04 2015-04-01 林成龙 Composition containing silver nanoparticles and application thereof
CN105213358A (en) * 2015-10-29 2016-01-06 广东工业大学 A kind of loading curcumin or the nano silver targeted drug-supplying system of curcumin derivate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104473965A (en) * 2005-08-04 2015-04-01 林成龙 Composition containing silver nanoparticles and application thereof
CN101880381A (en) * 2010-06-30 2010-11-10 清华大学深圳研究生院 Segmented copolymer modified by polyethylene glycol 1000 vitamin E succinic acid ester, preparation method and applications thereof
CN104368010A (en) * 2014-11-26 2015-02-25 苏州纳思达生物医药有限公司 Application of vitamin E polyethylene glycol succinate and derivatives thereof in preparation of hydrogel nanoparticle preparation of prodrug of hydrophilic medicine
CN105213358A (en) * 2015-10-29 2016-01-06 广东工业大学 A kind of loading curcumin or the nano silver targeted drug-supplying system of curcumin derivate

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109908175A (en) * 2019-03-21 2019-06-21 华南农业大学 A kind of organic/inorganic joint bactericidal composition and the preparation method and application thereof
CN112336858A (en) * 2020-10-09 2021-02-09 华南理工大学 Bismuth-manganese-based composite particle and preparation method and application thereof
CN112336858B (en) * 2020-10-09 2021-09-21 华南理工大学 Bismuth-manganese-based composite particle and preparation method and application thereof
CN113304122A (en) * 2021-06-02 2021-08-27 浙江大学 Ts antibacterial peptide-TPGS modified composite nano drug delivery system and preparation and application thereof

Also Published As

Publication number Publication date
CN106727431B (en) 2019-10-29

Similar Documents

Publication Publication Date Title
Hammami et al. Gold nanoparticles: Synthesis properties and applications
Tabassum et al. Zirconium oxide (ZrO2) nanoparticles from antibacterial activity to cytotoxicity: A next-generation of multifunctional nanoparticles
Mondal et al. Biosynthesis of silver nanoparticles using culture supernatant of Shewanella sp. ARY1 and their antibacterial activity
Thenmozhi et al. Antifungal activity of Streptomyces sp. VITSTK7 and its synthesized Ag2O/Ag nanoparticles against medically important Aspergillus pathogens
CN106727431B (en) Polyethylene glycol vitamin E succinate modification carries tigecycline nano grain of silver and preparation and application
Mittal et al. Unveiling the cytotoxicity of phytosynthesised silver nanoparticles using Tinospora cordifolia leaves against human lung adenocarcinoma A549 cell line
Bafghi et al. Biosynthesis of selenium nanoparticles by Aspergillus flavus and Candida albicans for antifungal applications
Mandhata et al. Isolation, biosynthesis and antimicrobial activity of gold nanoparticles produced with extracts of Anabaena spiroides
CN103961712A (en) Superparamagnetic ferroferric oxide nano particle drug carrier, preparation method and application thereof
Zhao et al. Facile one-pot synthesis of multifunctional protamine sulfate-derived carbon dots for antibacterial applications and fluorescence imaging of bacteria
Alshawwa et al. In Situ biosynthesis of reduced alpha hematite (α-Fe2O3) nanoparticles by Stevia Rebaudiana L. leaf extract: Insights into antioxidant, antimicrobial, and anticancer properties
Luzala et al. A critical review of the antimicrobial and antibiofilm activities of green-synthesized plant-based metallic nanoparticles
Zhao et al. Preparation of two types of silver-doped fluorescent carbon dots and determination of their antibacterial properties
Lucío et al. Bactericidal effect of 5-Mercapto-2-nitrobenzoic Acid-Coated silver nanoclusters against multidrug-resistant Neisseria gonorrhoeae
Amna et al. Apoptosis induced by copper oxide quantum dots in cultured C2C12 cells via caspase 3 and caspase 7: a study on cytotoxicity assessment
Young et al. Chondroitin sulfate-stabilized silver nanoparticles: Improved synthesis and their catalytic, antimicrobial, and biocompatible activities
Tiwari et al. Molecular weight of polyethylenimine-dependent transfusion and selective antimicrobial activity of functional silver nanoparticles
Ortega et al. Polymer mediated synthesis of cationic silver nanoparticles as an effective anti-fungal and anti-biofilm agent against Candida species
Zhou et al. Preparation of fluorescently labeled chitosan-quercetin drug-loaded nanoparticles with excellent antibacterial properties
Khatua et al. CTAB‐PLGA Curcumin Nanoparticles: Preparation, Biophysical Characterization and Their Enhanced Antifungal Activity against Phytopathogenic Fungus Pythium ultimum
Zainab et al. Fluconazole and biogenic silver nanoparticles-based nano-fungicidal system for highly efficient elimination of multi-drug resistant Candida biofilms
Hashem et al. Sustainable biosynthesized bimetallic ZnO@ SeO nanoparticles from pomegranate peel extracts: antibacterial, antifungal and anticancer activities
Yadav et al. Grewia tenax‐Mediated Silver Nanoparticles as Efficient Antibacterial and Antifungal Agents
Xue et al. Amphotericin B and curcumin co-loaded porous microparticles as a sustained release system against Candida albicans
Preeti et al. Synthesis of nano-ZnO by chemical reduction method and their micro biocide activity against bacterial skin pathogens

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant