CN106725931A - A kind of tooth implant for the bioactivity surface containing antibacterial peptide - Google Patents
A kind of tooth implant for the bioactivity surface containing antibacterial peptide Download PDFInfo
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- CN106725931A CN106725931A CN201611207447.1A CN201611207447A CN106725931A CN 106725931 A CN106725931 A CN 106725931A CN 201611207447 A CN201611207447 A CN 201611207447A CN 106725931 A CN106725931 A CN 106725931A
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- tooth implant
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0012—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy
- A61C8/0013—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy with a surface layer, coating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0012—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy
- A61C8/0016—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy polymeric material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0018—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the shape
- A61C8/0022—Self-screwing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0018—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the shape
- A61C8/0028—Pins, needles; Head structures therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0018—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the shape
- A61C8/0037—Details of the shape
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
- A61L2300/608—Coatings having two or more layers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
Abstract
The present invention discloses a kind of tooth implant for the bioactivity surface containing antibacterial peptide, is specifically improving tooth implant structure simultaneously, and outer surface is improved, and constructs the tooth implant with different number of plies coatings.Its coating relies primarily on cation and anion electrostatic attraction each other, the molecular coatings of ordered arrangement is formed in the planting body surface of solids, because shitosan and AMP composite layers are the biomaterials with good biodegradability properties and biocompatibility.Due to the presence of top layer chondroitin sulfate layer antibacterial peptide layer lamination layer structure, with lasting, efficient sterilized, anti-infection ability, its antibacterial, anti-apoptotic activities, adhesion, survival, growth and the differentiation of various kinds of cell (such as Gegenbaur's cell, cartilage cell) can be supported, is had broad application prospects.
Description
Technical field
The invention belongs to tooth implant technical field, and in particular to a kind of tooth for the bioactivity surface containing antibacterial peptide
Planting body.
Background technology
With continuing to develop for tooth implant technology, and widely approved by doctor patient, be also to send out in dentistry in recent years
A most fast specialty of exhibition, constantly makes a breakthrough, for more tooth disease patients provide specialty help.However, not
It is that existing intervention device is easy to infect after implanting to obtain another present situation not recognized.Main infection mechanism is
Material surface is sticked to first into internal bacterium, and due to nutrient environment abundant in vivo, bacterium breeds, and forms one
The biomembrane of layer protectiveness.The presence of biomembrane causes that the infection that medical apparatus trigger is particularly refractory.
Roos.Jansaker etc. carries out follow-up for many years to plantation postoperative patient, and bleeding is examined while not occurring there is spy
Deossification carries out clinical case statistics for diagnostic criteria, as a result shows, the incidence of disease of peri-implant mucositis is about 79%；Very
To there are some researches show the planting body for about having more than 90% spy occurred and examines bleeding.And peri-implantitiss can cause peri-implant
The defect of bone tissue, forfeiture, such as treatment can cause the loose or dislocation of planting body not in time.
Biologically active peptide be in protein 20 natural amino acids with different compositions and arrangement mode constituted from dipeptides to
The general name of complicated linear, loop configuration different peptides, is derived from the multi-functional compounds of protein.Active peptide has various
Body metabolism and physiological regulation function, absorption easy to digest have immune promotion, hormone control, antibacterial, antiviral, hypotensive, drop blood
Fat etc. is acted on.
Because the main component of dental implant surface is generally processed as negatively charged state.And will be poly- containing electropositive groups
Compound such as shitosan has preferable adhesion with dental implant surface.In addition containing with hydroxyl hydrogen-bond donor/acceptor each other
The polymer of group, such as sodium alginate, hydroxypropyl cellulose are likely to have certain adhesion with dental implant surface.
So, surface nature requirement of the tooth implant to material is very high.Therefore, new dental implant surface is explored to change
Property method has been increasingly becoming the direction of scientific rersearch of field of planting.
The content of the invention
For the technical scheme that solution above-mentioned technical problem is used is：Disclose a kind of for the bioactivity table containing antibacterial peptide
The tooth implant in face, manual tooth planting's external surface is provided with screw thread, and the implanting body of artificial tooth two ends are respectively neck
And head, it is characterised in that helical form self tapping groove, the spiral of the helical form self tapping groove are provided with the outer surface of the head
Direction is identical with hand of thread spiral, and the lead angle of the helical form self tapping groove is different from the lead angle of screw thread, described anti-
To be covered in thread surface, basic unit is titanium dioxide nanotube array layer to bacterium peptide layer, then by positive charge chitosan layer and negative electricity
Lotus AMP composite layers are alternately assembled, the structure of outer layer load chondroitin sulfate layer and antibacterial peptide layer composition.
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, the antibacterial peptide layer includes following operating procedure：
(1) by surface for the tooth implant cleaning of titanium dioxide nanotube array layer is standby；
(2) chitosan solution of 0.5~5mg/ml is prepared：Concentration is 1mg/mlAMP complex solutions；
(3) by the tooth implant described in step (1) be immersed in step (2) gained chitosan solution in 10~20min so that
Dental implant surface becomes positively charged lotus；Fully cleaned with deionized water again；
(4) it is 5~10min in 1mg/mlAMP complex solutions the tooth implant described in step (3) to be immersed in into concentration,
So that negative electrical charge on dental implant surface band；Fully cleaned with deionized water again；
The number of plies for assembling as needed, repeat step (3), (4) and (3) is that can obtain the tooth kind with different number of plies coatings
Implant；
(5) tooth implant described in step (4) is soaked in concentration is for the chondroitin sulfate sodium sulfate salt solution of 2mg/mL
5~15min of bubble, solution system control ph is 6, then is spontaneously dried after fully being cleaned with deionized water；Sulfuric acid is coated with again
It is 7 that the tooth implant of chondroitin is soaked in pH value, and concentration is 5~15min in the antibacterial peptide solution of 0.5mg/mL, then uses deionization
Water is spontaneously dried after fully cleaning, and acquisition is coated with the material of chondroitin sulfate layer-antibacterial peptide layer composite bed.
In above-mentioned technical proposal, the AMP is cationic antimicrobial small peptide, English name:cationic
antimicrobial peptides。
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, described cleaning refers to deionized water, and ethanol and acetone are cleaned by ultrasonic, after being dried up with nitrogen, are put in vacuum drying chamber,
Dried 24 hours under conditions of 60 DEG C.
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, the deacetylation of described shitosan is that 50~98%, Mw is 100~1500kDa.
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, during described chitosan solution is the acetic acid solution for dissolve chitosan in 0.3~3%, it is made into the shell of 0.5~5mg/ml
Glycan solution, resulting solution is mixed with atoleine, and volume ratio is 0.1~0.4, and it is 0.5~1% to add volumetric concentration
Sorbester p17, while adding dispersant magnesium stearate, sorbester p17 is 2~4: 1 with the mass ratio of magnesium stearate, stirring gained Water-In-Oil
Emulsion, is added thereto to the crosslinking agent vanillic aldehyde 1ml of 5~10mg/ml, is reacted 4~6 hours under 40 DEG C~60 DEG C stirrings
Afterwards；Standing takes supernatant；The chitosan particle of gained is added into a small amount of glacial acetic acid solution, magnetic stirring apparatus with aqueous suspension again
Under stirring condition, NaCl is added, its final concentration is reached 0.15M；The solution is positively charged.
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, the standing takes supernatant and refers to, stands more than 12 hours oil-water separation phases, takes lower aqueous solution, and regulation pH value is 8~
9, sediment is centrifuged off, take supernatant and obtain chitosan particle suspension.
For above-mentioned technical proposal, it is preferred in the case of, the tooth implant of the described bioactivity surface containing antibacterial peptide,
Wherein, weigh 50mg AMP composite powders to add to after the sterilizing of 50ml in deionized water, stirring is on the rocks fully to being completely dissolved
It is 5.0 that acetic acid adjusts pH, and this solution carries positive charge.
For above-mentioned technical proposal, it is preferred in the case of, the self tapping groove is made up of two arc surfaces, two arc surfaces
Binding site is tangent connection；The self tapping groove number is two or more, and described two above self tapping grooves are distributed on the outer of head
Surface；The head is frustum, and near neck, the head is circular arc away from one end of neck to the bigger diameter end of the head frustum
Face；The neck is frustum, and the bigger diameter end of the neck frustum is near head；The depth of the self tapping groove is from the small of head frustum
Footpath end gradually becomes shallower as to the bigger diameter end of head frustum.
For above-mentioned technical proposal, it is preferred in the case of, the blind hole is coaxial with planting body, and the blind hole is including coaxially suitable
The conical bore of secondary connection, quincunx hole, cylindrical hole and screwed hole, the conical bore are close to blind hole openend, and the conical bore is
Meet the conical bore in Morse's cone face；The quincunx hole is that cross section is quincunx through hole.
Character of innovation of the invention is：
The tooth implant with different number of plies coatings prepared by the present invention.Its coating relies primarily on cation and anion
Electrostatic attraction each other, the molecular coatings of ordered arrangement are formed in the planting body surface of solids, because shitosan and AMP are compound
Thing is the biomaterial with good biodegradability properties and biocompatibility.Because top layer chondroitin sulfate layer-antibacterial peptide layer is multiple
The presence of sheet combination structure, with lasting, efficient sterilized, anti-infection ability, its antibacterial, anti-apoptotic activities, it would be preferable to support various
Adhesion, survival, growth and the differentiation of cell (such as Gegenbaur's cell, cartilage cell), have broad application prospects.
Brief description of the drawings
Fig. 1 is the front view of planting body of the present invention；
Specific embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.Any one skilled in the art in the technical scope of present disclosure, according to
Technical scheme and its inventive concept carry out equivalent or change belongs to protection category of the present invention.
Configuration concentration is the AMP complex solutions of lmg/ml；
50mg AMP composite powders are weighed to add to after the sterilizing of 50ml in deionized water, stirring fully to being completely dissolved,
It is 5.0 that acetic acid on the rocks adjusts pH, and this solution carries positive charge.
Configuration concentration is 0.5mg/mL antibacterial peptide solutions
5mg antibacterial peptides are weighed, is dissolved in 10mL deionized waters, it is 7 to be prepared into pH value, and concentration is anti-for 0.5mg/mL
Bacterium peptide solution.
In following embodiments, implanting body of artificial tooth used has following architectural feature：
Manual tooth planting's external surface is provided with screw thread, as shown in figure 1, the plantation external surface is provided with screw thread,
Planting body two ends are respectively neck 6 and head 5, and helical form self tapping groove 2, helical form self tapping groove 2 are provided with the outer surface of head 5
The hand of spiral of the hand of spiral and screw thread be dextrorotation, the lead angle of the lead angle more than screw thread of helical form self tapping groove 2.
Self tapping groove 2 is made up of the first arc surface 3 and the second arc surface 4, and the binding site of the first arc surface 3 and the second arc surface 4 is phase
Connection is cut, the stress concentration that angle can be avoided to slot around the corner improves planting body intensity.The present embodiment head 5 is frustum,
The taper conical surface of head frustum and the angle of horizontal plane are about 80 °, the bigger diameter end of the frustum of head 5 near neck 6, head 5 away from
One end of neck 6 is arc surface 1；Neck 6 is frustum, and the bigger diameter end of the frustum of neck 6 is near head 5.The depth of self tapping groove 2 is from head
The miner diameter end of the frustum of portion 5 gradually becomes shallower as to the bigger diameter end of the frustum of head 5, with bone tissue after volume and the screw thread extruding of self tapping groove 2
Volume is consistent.To be covered in thread surface, basic unit is titanium dioxide nanotube array layer to the antibacterial peptide layer, then by positive charge
Chitosan layer and negative electrical charge AMP composite layers are alternately assembled, the structure of outer layer load chondroitin sulfate layer and antibacterial peptide layer composition.
Embodiment 1
(1) it is the tooth implant of titanium dioxide nanotube array layer by surface, successively with deionized water, ethanol and acetone are super
Sound is cleaned, and after being dried up with nitrogen, is put in vacuum drying chamber, is dried 24 hours under conditions of 60 DEG C, is placed standby；
(2) dissolve chitosan in 0.3% acetic acid solution, be made into the chitosan solution of 0.5mg/ml, described shell
The deacetylation of glycan is 1000kDa for 50%, Mw.
(3) step (2) resulting solution is mixed with atoleine, volume ratio is 0.1, and it is 0.5% to add volumetric concentration
Sorbester p17, while adding dispersant magnesium stearate, the mass ratio of sorbester p17 and magnesium stearate is 2: 1, is stirred 1 hour at room temperature
More than, form uniform water-in-oil emulsion；
(4) the crosslinking agent vanillic aldehyde 1ml of 5mg/ml, 40 DEG C of stirring shapes will be being added in step (3) gained water-in-oil emulsion
Reacted 4 hours under state, obtain chitosan particle；
(5) step (4) products therefrom is stood into more than 12 hours oil-water separation phases, takes lower aqueous solution, regulation pH value is
8, sediment is centrifuged off, take supernatant and obtain chitosan particle suspension
(6) to the chitosan particle suspension added in deionized water obtained by step (5), the glacial acetic acid solution of lml is added,
Under the conditions of magnetic stirrer, NaCl is added, its final concentration is reached 0.15M；The solution is positively charged；
Embodiment 2
(1) it is the tooth implant of titanium dioxide nanotube array layer by surface, successively with deionized water, ethanol and acetone are super
Sound is cleaned, and after being dried up with nitrogen, is put in vacuum drying chamber, is dried 24 hours under conditions of 60 DEG C, is placed standby；
(2) dissolve chitosan in 3% acetic acid solution, be made into the chitosan solution of 5mg/ml, described shitosan
Deacetylation for 98%, Mw be 1500kDa.
(3) step (2) resulting solution is mixed with atoleine, volume ratio is 0.4, and it is 1% to add volumetric concentration
Sorbester p17, while adding dispersant magnesium stearate, the mass ratio of sorbester p17 and magnesium stearate is 4: 1, stir at room temperature 1 hour with
On, form uniform water-in-oil emulsion；
(4) the crosslinking agent vanillic aldehyde 1ml of 10mg/ml, 60 DEG C of stirring shapes will be being added in step (3) gained water-in-oil emulsion
Reacted 6 hours under state, obtain chitosan particle；
(5) step (4) products therefrom is stood into more than 12 hours oil-water separation phases, takes lower aqueous solution, regulation pH value is
9, sediment is centrifuged off, take supernatant and obtain chitosan particle suspension
(6) to the chitosan particle suspension added in deionized water obtained by step (5), the glacial acetic acid solution of lml is added,
Under the conditions of magnetic stirrer, NaCl is added, its final concentration is reached 0.15M；The solution is positively charged；
Embodiment 3
The tooth implant that step (1) treatment in embodiment 1 is obtained is immersed in the shitosan described in example 1 above and 2
10~20min in solution, the lotus so that dental implant surface becomes positively charged；Fully cleaned with deionized water again；
By the tooth implant after above-mentioned cleaning be immersed in concentration mentioned above for 10 in 1mg/mlAMP complex solutions~
20min, so that negative electrical charge on dental implant surface band；Fully cleaned with deionized water again；
The tooth implant that treatment is obtained is immersed in 10~20min in the chitosan solution described in example 1 above and 2, with
Dental implant surface is set to become positively charged lotus；Fully cleaned with deionized water again；
The step of number of plies for assembling as needed, repetition embodiment 3 more than 2 times, you can obtain with different number of plies coatings
Tooth implant.
Embodiment 4
The tooth implant of different number of plies coatings will be as needed assembled in embodiment 3, in concentration for the sulfuric acid of 2mg/mL is soft
10~20min of immersion in ossein sodium sulfate salt solution (polysaccharide solution with negative electrical charge), solution system control ph is 6, then
Spontaneously dried after fully being cleaned with deionized water；
The material for having chondroitin sulfate will be coated and be soaked in pH value is 7, and concentration is in the antibacterial peptide solution of 0.5mg/mL
15min, then spontaneously dry after fully being cleaned with deionized water, acquisition is coated with chondroitin sulfate layer-antibacterial peptide layer composite bed
Material.
For the tooth implant that the above method is prepared, surface contact angle analysis is carried out, its result is from hydrophily and profit
The presence of coating is also demonstrate that on moist.Understood according to analysis, the Dental implantion with different number of plies coatings prepared by the present invention
Body.Its coating relies primarily on cation and anion electrostatic attraction each other, and row in order is formed in the planting body surface of solids
The molecular coatings of row, because shitosan and AMP compounds are the biomaterials with good biodegradability properties and biocompatibility.
Due to the presence of top layer chondroitin sulfate layer-antibacterial peptide layer lamination layer structure, with lasting, efficient sterilized, anti-infection ability,
Its antibacterial, anti-apoptotic activities, it would be preferable to support the adhesion of various kinds of cell (such as Gegenbaur's cell, cartilage cell), survival, growth with point
Change, have broad application prospects.
Claims (9)
1. a kind of tooth implant of the bioactivity surface containing antibacterial peptide, manual tooth planting's external surface is provided with screw thread,
The planting body two ends are respectively neck and head, it is characterised in that helical form self tapping is provided with the outer surface of the head
Groove, the hand of spiral of the helical form self tapping groove is identical with hand of thread spiral, the lead angle of the helical form self tapping groove with
The lead angle of screw thread is different, and to be covered in thread surface, basic unit is titanium dioxide nanotube array layer to the antibacterial peptide layer,
Alternately assembled by positive charge chitosan layer and negative electrical charge AMP composite layers again, outer layer loads chondroitin sulfate layer and antibacterial peptide layer
The structure of composition.
2. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：The antibacterial
The preparation method of peptide layer includes following operating procedure：
(1) by surface for the tooth implant cleaning of titanium dioxide nanotube array layer is standby；
(2) chitosan solution of 0.5~5mg/ml is prepared：Concentration is 1mg/mlAMP complex solutions；
(3) by the tooth implant described in step (1) be immersed in step (2) gained chitosan solution in 10~20min so that tooth kind
Implant surface becomes positively charged lotus；Fully cleaned with deionized water again；
(4) it is 5~10min in 1mg/mlAMP complex solutions the tooth implant described in step (3) to be immersed in into concentration, so that
Negative electrical charge on dental implant surface band；Fully cleaned with deionized water again；
The number of plies for assembling as needed, repeat step (3), (4) and (3) is that can obtain the Dental implantion with different number of plies coatings
Body；
(5) by the tooth implant described in step (4) concentration for 2mg/mL chondroitin sulfate sodium sulfate salt solution in immersion 5~
15min, solution system control ph is 6, then is spontaneously dried after fully being cleaned with deionized water；Chondroitin sulfate is coated with again
Tooth implant be soaked in pH value for 7, concentration is 5~15min in the antibacterial peptide solution of 0.5mg/mL, then abundant with deionized water
Spontaneously dried after cleaning, acquisition is coated with the material of chondroitin sulfate layer-antibacterial peptide layer composite bed.
3. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：Described is clear
It refers to deionized water to wash, and ethanol and acetone are cleaned by ultrasonic, and after being dried up with nitrogen, are put in vacuum drying chamber, in 60 DEG C of condition
Lower drying 24 hours.
4. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：Described shell
The deacetylation of glycan is that 50~98%, Mw is 100~1500kDa.
5. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：Described shell
During glycan solution is the acetic acid solution for dissolve chitosan in 0.3~3%, the chitosan solution of 0.5~5mg/ml is made into, by institute
Obtain solution to mix with atoleine, volume ratio is 0.1~0.4, and adds the sorbester p17 that volumetric concentration is 0.5~1%, while plus
Enter dispersant magnesium stearate, sorbester p17 is 2~4: 1 with the mass ratio of magnesium stearate, stirring gained water-in-oil emulsion adds thereto
Enter the crosslinking agent vanillic aldehyde 1ml of 5~10mg/ml, after being reacted 4~6 hours under 40 DEG C~60 DEG C stirrings；Standing takes supernatant
Liquid；The chitosan particle of gained is added into a small amount of glacial acetic acid solution with aqueous suspension again, under the conditions of magnetic stirrer, then
NaCl is added, its final concentration is reached 0.15M；The solution is positively charged.
6. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 5, it is characterised in that：The standing
Taking supernatant refers to, stands more than 12 hours oil-water separation phases, takes lower aqueous solution, and regulation pH value is 8~9, is centrifuged off sinking
Starch, takes supernatant and obtains chitosan particle suspension.
7. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：The concentration
For the collocation method of 1mg/mlAMP complex solutions is：Weigh the compound medicinal powders of 50mgAMP, be placed in after the sterilizing of 100ml go from
In sub- water, in sterile closed container, magnetic agitation to pulvis is completely dissolved, and this solution carries negative electrical charge.
8. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：The self tapping
Groove is made up of two arc surfaces, and two binding sites of arc surface are tangent connection；The self tapping groove number is two or more, institute
State the outer surface that two or more self tapping groove is distributed on head；The head is frustum, and the bigger diameter end of the head frustum is near neck
Portion, the head is arc surface away from one end of neck；The neck is frustum, and the bigger diameter end of the neck frustum is near head
Portion；The depth of the self tapping groove gradually becomes shallower as from the miner diameter end of head frustum to the bigger diameter end of head frustum.
9. the tooth implant of the bioactivity surface containing antibacterial peptide according to claim 1, it is characterised in that：The blind hole
Coaxial with planting body, the blind hole includes the conical bore, quincunx hole, cylindrical hole and the screwed hole that are coaxially sequentially connected with, the circle
Near blind hole openend, the conical bore is the conical bore for meeting Morse's cone face to taper hole；The quincunx hole is for cross section
Quincunx through hole.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110510878A (en) * | 2019-10-09 | 2019-11-29 | 山东健能陶瓷科技有限公司 | Ceramic glaze with antibacterial functions and preparation method thereof and ceramics |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102327645A (en) * | 2011-09-26 | 2012-01-25 | 浙江大学 | Preparation method of bionic coating carrying bioactive factors |
| CN103550004A (en) * | 2013-11-18 | 2014-02-05 | 大连三生科技发展有限公司 | Artificial dental implant, artificial dental implanting system and implanting method |
| CN104941002A (en) * | 2015-05-28 | 2015-09-30 | 重庆大学 | Preparation method of bacterial responsive titanium-based antibacterial implantation material for hyaluronidase secreting type bacteria |
| CN105126165A (en) * | 2015-01-21 | 2015-12-09 | 中国人民解放军第三〇七医院 | Titanium dental implant material and preparing method and application of titanium dental implant material |
| CN106178125A (en) * | 2016-07-20 | 2016-12-07 | 太原理工大学 | A kind of preparation method of nanometer hydroxyapatite chitosan compound microsphere |
-
2016
- 2016-12-23 CN CN201611207447.1A patent/CN106725931A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102327645A (en) * | 2011-09-26 | 2012-01-25 | 浙江大学 | Preparation method of bionic coating carrying bioactive factors |
| CN103550004A (en) * | 2013-11-18 | 2014-02-05 | 大连三生科技发展有限公司 | Artificial dental implant, artificial dental implanting system and implanting method |
| CN105126165A (en) * | 2015-01-21 | 2015-12-09 | 中国人民解放军第三〇七医院 | Titanium dental implant material and preparing method and application of titanium dental implant material |
| CN104941002A (en) * | 2015-05-28 | 2015-09-30 | 重庆大学 | Preparation method of bacterial responsive titanium-based antibacterial implantation material for hyaluronidase secreting type bacteria |
| CN106178125A (en) * | 2016-07-20 | 2016-12-07 | 太原理工大学 | A kind of preparation method of nanometer hydroxyapatite chitosan compound microsphere |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110510878A (en) * | 2019-10-09 | 2019-11-29 | 山东健能陶瓷科技有限公司 | Ceramic glaze with antibacterial functions and preparation method thereof and ceramics |
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