CN106725633B - A kind of genetic test special sampler - Google Patents
A kind of genetic test special sampler Download PDFInfo
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- CN106725633B CN106725633B CN201710019382.6A CN201710019382A CN106725633B CN 106725633 B CN106725633 B CN 106725633B CN 201710019382 A CN201710019382 A CN 201710019382A CN 106725633 B CN106725633 B CN 106725633B
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- compression bar
- noumenon
- ontology
- piston
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
Abstract
The invention discloses a kind of genetic test special samplers, including the first noumenon, second ontology, first piston, second piston, first, which takes out compression bar and second, takes out compression bar, penetrating fluid is equipped in the first noumenon and the second ontology, the first noumenon inner cavity is protruded into first piston one end, the other end is connected with first pumping compression bar one end, the second chamber body is protruded into second piston one end, the other end is connected with second pumping compression bar one end, first pumping compression bar and the second pumping compression bar stretch out body part and pass through shaft interconnection, and first takes out between compression bar and the second pumping compression bar equipped with reset spring, macromolecule permeable membrane is equipped in the first noumenon and second ontology one end, the configuration of the present invention is simple, it is easy to operate, intraoral saliva cell and upper jaw cast-off cells can be acquired simultaneously, it and is not in cross-infection in operating process, and will not counterpart it is intracavitary Portion's skin damages.
Description
Technical field
The present invention relates to gene sampling technical field, specially a kind of genetic test special sampler.
Background technique
Genetic engineering is by importing in recipient cell after foreign gene is passed through vitro recombination, to enable this gene
It replicates, transcribe, accurate translation in recipient cell.Genetic engineering is with artificial method by required a certain donor organism
--- DNA is extracted, after in vitro is cut with proper implements enzyme, using it and as the DNA of carrier
Molecule connects, and then imports together with carrier in a certain recipient cell for being easier to growth, breeding, to allow allogenic material at it
It is middle normally to be replicated and expressed, to obtain a kind of brand-new technology of new species.Currently, there is a kind of acquisition DNA gene
Method, using swab scraping oral epithelium tissue cell and be applied on sheet glass, carry out genetic analysis, but this side
The hand that method operates cotton balls is easy pollution, causes cross-infection, and larger using the method testing result error.
Summary of the invention
It is mentioned above in the background art to solve the purpose of the present invention is to provide a kind of genetic test special sampler
Problem.
To achieve the above object, the invention provides the following technical scheme: a kind of genetic test special sampler, including
One ontology, the second ontology, first piston, second piston, first take out compression bar and second and take out compression bar, the first noumenon and described the
Penetrating fluid is equipped in two ontologies, the first noumenon inner cavity is protruded into described first piston one end, and the other end and first take out compression bar one end
It is connected, the second chamber body is protruded into described second piston one end, and the other end is connected with second pumping compression bar one end, and described first takes out pressure
Bar and described second takes out compression bar stretching the first noumenon and the second body part by shaft interconnection, and described first takes out compression bar
It is equipped with reset spring between the second pumping compression bar, is equipped with macromolecule in the first noumenon and second ontology one end
Permeable membrane.
Preferably, the penetrating fluid mainly includes sodium lactate 1-5 parts, 10-15 parts of glucose, sodium chloride 5- in parts by weight
10 parts, 4-8 parts of magnesium chloride and 30-40 parts of water.
Preferably, the macromolecule permeable membrane is prepared by the raw material of following portions by weight: 30-50 parts of non-woven fabrics, two
5-10 parts of methylformamide, 1-3 parts of polysulfones, 2-6 parts of inositol, 3-8 parts of sorbierite, 8-12 parts of isothiazolinone and sericin
10-15 parts of aqueous solution.
Preferably, the macromolecule permeable membrane preparation process the following steps are included:
A, it after mixing dimethylformamide, polysulfones, inositol at normal temperature, stirs evenly, casting solution is made；
B, casting solution is coated on non-woven fabrics and forms support counterdie；
C, inositol, sorbierite, isothiazolinone and sericin aqueous solution are mixed in 30 DEG C of reaction kettle, and
It is stirred continuously, mixing time 30min stands 10min later, obtains macromolecule mixture；
D, macromolecule mixture is sprayed on support counterdie, and be put into film-making machine, after film-making machine is warming up to 130 DEG C
Polymeric chemical reaction occurs, permeable membrane is made.
Preferably, sampler application method the following steps are included:
A, it holds first and takes out compression bar and the second pumping compression bar cross section, the first noumenon and the second ontology are protruded into oral cavity；
B, macromolecule permeable membrane is close to tongue surface and maxillary surface respectively；
C, it stirs the first pumping compression bar and second and takes out compression bar, pull out first piston and second piston respectively；
D, tongue surface and maxillary surface cell are sucked high score by suction function by the first noumenon and the second body interior
On sub- permeable membrane, enter in penetrating fluid by osmosis, completes sampling.
Compared with prior art, the beneficial effects of the present invention are:
(1) the configuration of the present invention is simple, easy to operate, can acquire intraoral saliva cell simultaneously and the upper jaw falls off carefully
Born of the same parents, and be not in cross-infection in operating process, and cavity interior skin will not be damaged.
(2) the macromolecule permeable membrane manufacture craft that the present invention uses is simple, and producing principle is environment friendly and pollution-free, and infiltration obtained
Permeable membrane also has antibacterial, bactericidal effect, further prevents occurring cross-infection in oral cavity.
Detailed description of the invention
Fig. 1 is overall structure diagram of the invention；
In figure: 1, the first noumenon；2, the second ontology；3, first piston；4, second piston；5, first compression bar is taken out；6, second
Take out compression bar；7, penetrating fluid；8, shaft；9, reset spring；10, macromolecule permeable membrane.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of genetic test special sampler, including the first noumenon
1, the second ontology 2, first piston 3, second piston 4, first take out compression bar 5 and second and take out compression bar 6, the first noumenon 1 and the second ontology 2
It is inside equipped with penetrating fluid 7,1 inner cavity of the first noumenon is protruded into 3 one end of first piston, and the other end is connected with first pumping 5 one end of compression bar, the
2 inner cavity of the second ontology is protruded into two pistons, 3 one end, and the other end is connected with second pumping 6 one end of compression bar, and first, which takes out compression bar 5 and second, takes out
Compression bar 6 stretches out the first noumenon 1 and 2 part of the second ontology by 8 interconnection of shaft, and first takes out compression bar 5 and the second pumping compression bar 6
Between be equipped with reset spring 9, be equipped with macromolecule permeable membrane 10 in the first noumenon 1 and 2 one end of the second ontology.
Penetrating fluid 7 mainly includes sodium lactate 1-5 parts, 10-15 parts of glucose, 5-10 parts of sodium chloride, chlorination in parts by weight
4-8 parts of magnesium and 30-40 parts of water；Macromolecule permeable membrane 10 is prepared by the raw material of following portions by weight: non-woven fabrics 30-50
Part, 5-10 parts of dimethylformamide, 1-3 parts of polysulfones, 2-6 parts of inositol, 3-8 parts of sorbierite, 8-12 parts of isothiazolinone and silk
10-15 parts of glue protein aqueous solution.
Embodiment one:
Penetrating fluid 7 in parts by weight mainly include 2 parts of sodium lactate, 12 parts of glucose, 6 parts of sodium chloride, 5 parts of magnesium chloride and
32 parts of water；Macromolecule permeable membrane 10 is prepared by the raw material of following portions by weight: 30 parts of non-woven fabrics, 5 parts of dimethylformamide,
1 part of polysulfones, 2 parts of inositol, 3 parts of sorbierite, 8 parts of isothiazolinone and 10 parts of sericin aqueous solution.
In the present embodiment, the preparation process of macromolecule permeable membrane the following steps are included:
A, it after mixing dimethylformamide, polysulfones, inositol at normal temperature, stirs evenly, casting solution is made；
B, casting solution is coated on non-woven fabrics and forms support counterdie；
C, inositol, sorbierite, isothiazolinone and sericin aqueous solution are mixed in 30 DEG C of reaction kettle, and
It is stirred continuously, mixing time 30min stands 10min later, obtains macromolecule mixture；
D, macromolecule mixture is sprayed on support counterdie, and be put into film-making machine, after film-making machine is warming up to 130 DEG C
Polymeric chemical reaction occurs, permeable membrane is made.
In the present embodiment, the application method of sampler the following steps are included:
A, it holds first and takes out compression bar 5 and the second pumping 6 cross section of compression bar, the first noumenon 1 and the second ontology 2 are protruded into oral cavity
It is interior；
B, macromolecule permeable membrane 10 is close to tongue surface and maxillary surface respectively；
C, it stirs the first pumping compression bar 5 and second and takes out compression bar 6, pull out first piston 3 and second piston 4 respectively；
D, tongue surface and maxillary surface cell are sucked high inside the first noumenon 1 and the second ontology 2 by suction function
On molecule infiltration film, enter in penetrating fluid 7 by osmosis, completes sampling.
Embodiment two:
Penetrating fluid 7 mainly includes sodium lactate 1-5 parts, 10-15 parts of glucose, 5-10 parts of sodium chloride, chlorination in parts by weight
4-8 parts of magnesium and 30-40 parts of water；Macromolecule permeable membrane 10 is prepared by the raw material of following portions by weight: 50 parts of non-woven fabrics, two
10 parts of methylformamide, 3 parts of polysulfones, 6 parts of inositol, 8 parts of sorbierite, 12 parts of isothiazolinone and sericin aqueous solution 15
Part.
In the present embodiment, the preparation process of macromolecule permeable membrane the following steps are included:
A, it after mixing dimethylformamide, polysulfones, inositol at normal temperature, stirs evenly, casting solution is made；
B, casting solution is coated on non-woven fabrics and forms support counterdie；
C, inositol, sorbierite, isothiazolinone and sericin aqueous solution are mixed in 30 DEG C of reaction kettle, and
It is stirred continuously, mixing time 30min stands 10min later, obtains macromolecule mixture；
D, macromolecule mixture is sprayed on support counterdie, and be put into film-making machine, after film-making machine is warming up to 130 DEG C
Polymeric chemical reaction occurs, permeable membrane is made.
In the present embodiment, the application method of sampler the following steps are included:
A, it holds first and takes out compression bar 5 and the second pumping 6 cross section of compression bar, the first noumenon 1 and the second ontology 2 are protruded into oral cavity
It is interior；
B, macromolecule permeable membrane 10 is close to tongue surface and maxillary surface respectively；
C, it stirs the first pumping compression bar 5 and second and takes out compression bar 6, pull out first piston 3 and second piston 4 respectively；
D, tongue surface and maxillary surface cell are sucked high inside the first noumenon 1 and the second ontology 2 by suction function
On molecule infiltration film, enter in penetrating fluid 7 by osmosis, completes sampling.
Embodiment three:
Penetrating fluid 7 in parts by weight mainly include 3 parts of sodium lactate, 13 parts of glucose, 7 parts of sodium chloride, 6 parts of magnesium chloride and
35 parts of water；Macromolecule permeable membrane 10 is prepared by the raw material of following portions by weight: 40 parts of non-woven fabrics, 8 parts of dimethylformamide,
2 parts of polysulfones, 4 parts of inositol, 6 parts of sorbierite, 10 parts of isothiazolinone and 12 parts of sericin aqueous solution.
In the present embodiment, the preparation process of macromolecule permeable membrane the following steps are included:
A, it after mixing dimethylformamide, polysulfones, inositol at normal temperature, stirs evenly, casting solution is made；
B, casting solution is coated on non-woven fabrics and forms support counterdie；
C, inositol, sorbierite, isothiazolinone and sericin aqueous solution are mixed in 30 DEG C of reaction kettle, and
It is stirred continuously, mixing time 30min stands 10min later, obtains macromolecule mixture；
D, macromolecule mixture is sprayed on support counterdie, and be put into film-making machine, after film-making machine is warming up to 130 DEG C
Polymeric chemical reaction occurs, permeable membrane is made.
In the present embodiment, the application method of sampler the following steps are included:
A, it holds first and takes out compression bar 5 and the second pumping 6 cross section of compression bar, the first noumenon 1 and the second ontology 2 are protruded into oral cavity
It is interior；
B, macromolecule permeable membrane 10 is close to tongue surface and maxillary surface respectively；
C, it stirs the first pumping compression bar 5 and second and takes out compression bar 6, pull out first piston 3 and second piston 4 respectively；
D, tongue surface and maxillary surface cell are sucked high inside the first noumenon 1 and the second ontology 2 by suction function
On molecule infiltration film, enter in penetrating fluid 7 by osmosis, completes sampling.
Experimental example:
Antibacterial desalination experiment is carried out to reverse osmosis membrane made from ordinary RO membrane and various embodiments of the present invention, obtains data
It is as follows:
| Salt rejection rate (%) | Sterilizing rate (%) | |
| Common permeable film | 90.5 | 35.5 |
| Embodiment one | 98.9 | 92.5 |
| Embodiment two | 99.1 | 92.9 |
| Embodiment three | 99.5 | 93.5 |
As above table data it is found that permeable membrane made from embodiment three has optimum efficiency.
It is the configuration of the present invention is simple, easy to operate, intraoral saliva cell and upper jaw cast-off cells can be acquired simultaneously,
And is not in cross-infection in operating process, and cavity interior skin will not be damaged；In addition the present invention uses
Macromolecule permeable membrane manufacture craft it is simple, producing principle is environment friendly and pollution-free, and permeable membrane obtained also have antibacterial, sterilization make
With further preventing occurring cross-infection in oral cavity.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (1)
1. a kind of genetic test special sampler, including the first noumenon (1), the second ontology (2), first piston (3), the second work
Fill in (4), the first pumping compression bar (5) and second takes out compression bar (6), it is characterised in that: the first noumenon (1) and second ontology
(2) it is equipped with penetrating fluid (7) in, the first noumenon (1) inner cavity is protruded into described first piston (3) one end, and the other end and first, which are taken out, presses
Bar (5) one end is connected, and the second ontology (2) inner cavity is protruded into described second piston (3) one end, and the other end and second take out compression bar (6) one
End is connected, and first pumping compression bar (5) and second pumping compression bar (6) stretching the first noumenon (1) and the second ontology (2) part are led to
Shaft (8) interconnection is crossed, and is equipped with reset spring (9) between first pumping compression bar (5) and second pumping compression bar (6),
Macromolecule permeable membrane (10) are equipped in the first noumenon (1) and second ontology (2) one end；
The penetrating fluid (7) mainly includes sodium lactate 1-5 parts, 10-15 parts of glucose, 5-10 parts of sodium chloride, chlorine in parts by weight
Change magnesium 4-8 parts and 30-40 parts of water；
The macromolecule permeable membrane (10) is prepared by the raw material of following portions by weight: 30-50 parts of non-woven fabrics, dimethyl formyl
5-10 parts of amine, 1-3 parts of polysulfones, 2-6 parts of inositol, 3-8 parts of sorbierite, 8-12 parts of isothiazolinone and sericin aqueous solution
10-15 parts；
The preparation process of the macromolecule permeable membrane the following steps are included:
A, it after mixing dimethylformamide, polysulfones, inositol at normal temperature, stirs evenly, casting solution is made；
B, casting solution is coated on non-woven fabrics and forms support counterdie；
C, inositol, sorbierite, isothiazolinone and sericin aqueous solution are mixed in 30 DEG C of reaction kettle, and constantly
Stirring, mixing time 30min stand 10min later, obtain macromolecule mixture；
D, macromolecule mixture is sprayed on support counterdie, and be put into film-making machine, occurred after film-making machine is warming up to 130 DEG C
Polymeric chemical reaction, is made permeable membrane；
The application method of the genetic test special sampler the following steps are included:
A, it holds first and takes out compression bar (5) and second pumping compression bar (6) cross section, the first noumenon (1) and the second ontology (2) are protruded into
In oral cavity；
B, macromolecule permeable membrane (10) is close to tongue surface and maxillary surface respectively；
C, it stirs the first pumping compression bar (5) and second and takes out compression bar (6), pull out first piston (3) and second piston (4) respectively；
D, the first noumenon (1) and the second ontology (2) are internal by suction function, tongue surface and maxillary surface cell are sucked high
On molecule infiltration film, enter in penetrating fluid (7) by osmosis, completes sampling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710019382.6A CN106725633B (en) | 2017-01-11 | 2017-01-11 | A kind of genetic test special sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710019382.6A CN106725633B (en) | 2017-01-11 | 2017-01-11 | A kind of genetic test special sampler |
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| CN106725633A CN106725633A (en) | 2017-05-31 |
| CN106725633B true CN106725633B (en) | 2019-07-09 |
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Citations (8)
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| CN1535182A (en) * | 2001-04-30 | 2004-10-06 | Reagent delivery system | |
| CN101406388A (en) * | 2007-10-09 | 2009-04-15 | 刘旭 | Vaginal speculum |
| CN201996588U (en) * | 2011-03-28 | 2011-10-05 | 李学农 | Novel mouth sampling device for children |
| CN102527252A (en) * | 2012-01-11 | 2012-07-04 | 杭州天创环境科技股份有限公司 | Antibacterial composite reverse osmosis membrane |
| CN102527253A (en) * | 2012-01-11 | 2012-07-04 | 杭州天创环境科技股份有限公司 | Antibacterial antioxidative composite reverse osmosis membrane |
| CN102716681A (en) * | 2012-06-01 | 2012-10-10 | 森泰环保（湖南）科技有限公司 | Reverse osmosis membrane and manufacturing method for reverse osmosis membrane |
| CN103356243A (en) * | 2012-03-27 | 2013-10-23 | 马广 | Novel skin sampler |
| CN104840224A (en) * | 2015-05-29 | 2015-08-19 | 齐天杰 | Respiratory tract lower airway droplet collector |
-
2017
- 2017-01-11 CN CN201710019382.6A patent/CN106725633B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1535182A (en) * | 2001-04-30 | 2004-10-06 | Reagent delivery system | |
| CN101406388A (en) * | 2007-10-09 | 2009-04-15 | 刘旭 | Vaginal speculum |
| CN201996588U (en) * | 2011-03-28 | 2011-10-05 | 李学农 | Novel mouth sampling device for children |
| CN102527252A (en) * | 2012-01-11 | 2012-07-04 | 杭州天创环境科技股份有限公司 | Antibacterial composite reverse osmosis membrane |
| CN102527253A (en) * | 2012-01-11 | 2012-07-04 | 杭州天创环境科技股份有限公司 | Antibacterial antioxidative composite reverse osmosis membrane |
| CN103356243A (en) * | 2012-03-27 | 2013-10-23 | 马广 | Novel skin sampler |
| CN102716681A (en) * | 2012-06-01 | 2012-10-10 | 森泰环保（湖南）科技有限公司 | Reverse osmosis membrane and manufacturing method for reverse osmosis membrane |
| CN104840224A (en) * | 2015-05-29 | 2015-08-19 | 齐天杰 | Respiratory tract lower airway droplet collector |
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| CN106725633A (en) | 2017-05-31 |
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Address after: Room 408-1, 4th Floor, Incubator 5 Building, Science Park, Wenzhou National University, No. 50 Dongfangnan Road, Ouhai Economic Development Zone, Wenzhou City, Zhejiang Province, 325000 Applicant after: Wenzhou Zhongyi Technology Research Institute Co., Ltd. Address before: Room 6011, Block B, 188 Lanjiang Road, Wenzhou High-tech Industrial Development Zone, Zhejiang Province, 325000 Applicant before: Wenzhou Zhongyi Technology Service Co., Ltd. |
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Inventor after: Xu Xianshu Inventor after: Xu Yimeng Inventor after: Fang Rui Inventor after: Zheng Lifang Inventor after: Liu Panpan Inventor before: Xu Xian Shu |
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Effective date of registration: 20211122 Address after: 401122 No. 256, fangzheng Avenue, Beibei District, Shuitu street, Chongqing Patentee after: Chongqing Liangyi Biotechnology Co.,Ltd. Address before: Room 408-1, 4th Floor, Incubator 5 Building, Science Park, Wenzhou National University, No. 50 Dongfangnan Road, Ouhai Economic Development Zone, Wenzhou City, Zhejiang Province, 325000 Patentee before: WENZHOU ZHONGYI TECHNOLOGY RESEARCH INSTITUTE Co.,Ltd. |