CN106720176A - A kind of method that utilization electron beam irradiation ensures quality of large yellow croaker in Cold Chain Logistics transport - Google Patents
A kind of method that utilization electron beam irradiation ensures quality of large yellow croaker in Cold Chain Logistics transport Download PDFInfo
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- CN106720176A CN106720176A CN201611247950.XA CN201611247950A CN106720176A CN 106720176 A CN106720176 A CN 106720176A CN 201611247950 A CN201611247950 A CN 201611247950A CN 106720176 A CN106720176 A CN 106720176A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/015—Preserving by irradiation or electric treatment without heating effect
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/06—Freezing; Subsequent thawing; Cooling
Abstract
The invention belongs to food preservative technology field, and in particular to a kind of method that utilization electron beam irradiation ensures quality of large yellow croaker in Cold Chain Logistics transport, comprise the following steps：（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electron beam irradiation dosage is 1 4kGy；（3）Storage：By step（2）The middle rheum officinale fish sample through electron beam irradiation is placed in 18 0 DEG C -18 0 DEG C Cold Chain Logistics storages under temperature match curing conditions twice.The inventive method can effectively keep the quality of large yellow croaker, improve the cold storing and fresh-keeping time.
Description
Technical field
The invention belongs to food preservative technology field, and in particular to one kind ensures Cold Chain Logistics transport using electron beam irradiation
The method of middle quality of large yellow croaker.
Background technology
Large yellow croaker（Pseudosciaena crocea）Category Osteichthyes, Perciformes, Sciaenidae, yellow croaker category, are China
Traditional economy fish, because of delicious meat, rich in protein, fat, trace element etc., with anti-aging, resist immunity of organism
The effects such as power and be widely loved by the people.
The large yellow croaker of supermarket of China sale has two kinds:Chilled large yellow croaker and freezing large yellow croaker.At present, to chilled large yellow croaker
The research of preservation method, shelf life etc. is a lot, during such as Yang Xian under the conditions of 3 ± 0.5 DEG C, have studied gamma irradiation to iced storage
The influence of quality of large yellow croaker and shelf life, by total plate count, pseudomonad, produce vulcanization hydrogen bacteria, VBN and
The measure of trimethylamine, it is determined that fresh-keeping effect preferably, by shelf life extension to 30d, and can not have fat when irradiation dose is 1kGy
The taste that fat becomes sour；Li Tingting etc. at 4 DEG C, with the fresh-keeping large yellow croaker of the chitosan coating of various concentrations, it was demonstrated that 1.5% shitosan
Film can make large yellow croaker shelf life extension to 12d, can preferably keep large yellow croaker organoleptic quality；Will is worn far to wait in 4 ± 1 DEG C of conditions
Under, confirmed by the controlled atmospheric packing of gas with various ratio, when gas proportioning is 75%CO2/25%N2When, large yellow croaker shelf life is reachable
15d, 8d is extended than air-packing.But the research for freezing quality of large yellow croaker is little.Freezing large yellow croaker is by directly quick-frozen
Afterwards, then through Cold Chain Logistics stored and transported and sold, its shelf life is considerably longer than chilled large yellow croaker.Cold Chain Logistics refer to refrigerating class
Under low temperature environment of the food in links such as production and processing, storage, transport and sale all the time in regulation, to ensure food quality
Special supply chain system.Specify in Food Cold Chain Logistics technology and specification, quick-frozen aquatic product transportation preservation temperature should be maintained at -18
Below DEG C.But China's Cold Chain Logistics storaging and transport technology is not perfect, it is difficult to accomplish all the time within prescribed limit, cold chain transportation with
And during Storage in cold bank, due to the reason such as technology imperfection or freezer failure, aquatic products storage temperature can all sent out
It is raw to change, so as to influence its quality.The farsighted grade of glutinous rice flour dumplings is by simulating different logistics progresses, it was demonstrated that temperature fluctuation can influence tuna
Quality, and change frequency is more, flesh of fish deterioration is more notable；Boonsumrej etc. is studied tiger fur shrimp temperature fluctuation, as a result
Display is with the increase of number of freezing and thawing, the increase of meat fiber spacing；Sigurgisladottir etc. studies freeze/thaw to three texts
The influence of the microstructure of fish, finds the muscle fibers contract by the salmon of freeze thawing, and extracellular space is than fresh salmon
Greatly；Li Jinping show that temperature change can make beef color change by studying multigelation beef, influence physicochemical property,
Microstructure, PD etc., reduce its quality.Therefore, the fluctuation of temperature is noted in storage and transport process, to quality of large yellow croaker
Influence has important realistic meaning.But it is annual to become because aquatic products are corrupt caused by the failure of appearance in Cold Chain Logistics storage and transport process
The case of matter is still too numerous to enumerate, and certain loss is caused to China's economy.Irradiated targets technology as a kind of sterilization method,
Food can be rapidly and uniformly penetrated, by energy transmission, chemistry, physics and biological effect is produced in food, make hereditary thing
Matter loses replication capacity, so as to reach the effect of Pesticidal and sterilizing, so that on the basis of the quality for keeping food to greatest extent,
Extend the shelf life of food.If irradiation technique can be applied in frozen fish, to prevent the failure in cold chain transportation, one
Determine to slow down due to the harmful effect that temperature fluctuation brings in degree.
The content of the invention
In order to make up the deficiencies in the prior art, the technical problems to be solved by the invention are to provide a kind of using electron beam spoke
Electronic beam irradiation technology can be effectively applied to rheum officinale by the method according to quality of large yellow croaker in Cold Chain Logistics transport is ensured, the method
Fish preservation field.
In order to solve the above technical problems, the method that utilization electron beam irradiation provided by the present invention stores large yellow croaker, including
Following steps：
（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；
（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electricity
Beamlet irradiation dose is 1-4 kGy；
（3）Storage：By step（2）The middle rheum officinale fish sample through electron beam irradiation is placed in -18-0 DEG C--18-0 DEG C, twice alternating temperature
Under the conditions of Cold Chain Logistics storage.
Further, the electron beam irradiation dosage is 1,2 or 4kGy, preferably 2 kGy.
Further, the step（3）The reserve temperature of middle rheum officinale fish sample is -18 DEG C.
Beneficial effect of the present invention：The present invention using electron beam irradiation under specific irradiation dose to rheum officinale fish sample at
Reason, and the rheum officinale fish sample after treatment is placed in -18-0 DEG C--18-0 DEG C stores under temperature match curing conditions twice, by through this hair
Bright method treated rheum officinale fish sample VBN, total plate count, thiobarbituricacidα-, Electronic Nose, amino acid etc. refer to
Mark is timed detection, it is found that the inventive method can effectively keep the quality of large yellow croaker, improves the cold storing and fresh-keeping time.
Brief description of the drawings
Influence of Fig. 1 difference storage conditions to large yellow croaker total plate count；
Influence of Fig. 2 difference storage conditions to large yellow croaker VBN；
Fig. 3 difference storage conditions are to large yellow croaker barbital acid number（TBARS）Influence；
Influence of Fig. 4 difference irradiation doses to large yellow croaker total plate count；
Fig. 5 difference irradiation doses are to large yellow croaker barbital acid number（TBARS values）Influence；
PCA analysis of Fig. 6 difference irradiation doses to large yellow croaker Electronic Nose response；
Radar map of Fig. 7 difference irradiation doses to large yellow croaker Electronic Nose response.
Specific embodiment
The present invention is further described in detail with reference to specific embodiment.Heretofore described embodiment is only used for
It is bright to explain the present invention without being construed as limiting to the scope of the present invention.One of ordinary skill in the art can be from disclosed by the invention interior
Hold all deformations directly derived or associate, be considered as protection scope of the present invention.
Embodiment 1：
（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；
（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electricity
Beamlet irradiation dose is 1 kGy；
（3）Storage：By step（2）The middle rheum officinale fish sample through electron beam irradiation is placed in -18-0 DEG C--18-0 DEG C alternating temperature bar twice
Cold Chain Logistics storage under part.
Embodiment 2：
（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；
（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electricity
Beamlet irradiation dose is 2 kGy；
（3）Storage：By step（2）Cold Chain Logistics are stored under the conditions of the middle rheum officinale fish sample through electron beam irradiation is placed in -18 DEG C.
Embodiment 3：
（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；
（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electricity
Beamlet irradiation dose is 4 kGy；
（3）Storage：By step（2）The middle rheum officinale fish sample through electron beam irradiation is placed in -18-0 DEG C--18-0 DEG C alternating temperature bar twice
Cold Chain Logistics storage under part.
Test example：
1 materials and methods
1.1 materials
Fresh large yellow croaker is purchased from Wenzhou Pu Ting seafood market, is divided into six groups.One of which is placed in -18 DEG C of freezing storages all the time
Hide, another set is taken out at the 12nd day and the 26th day from -18 DEG C, is placed in and is thawed at room temperature, and hairtail temperature is down to 0
DEG C when, 24h is preserved under the conditions of 0 DEG C, remaining time preserves at -18 DEG C.Remaining four groups are stored in bubble chamber with ice bag
In, transporting Shanghai Academy of Agricultural Sciences to immediately carries out the radiation treatment of 10 MeV electron accelerators.Rheum officinale fish sample is laid in iron pan
In, the electron beam irradiation that dosage is 0,1,2,4 kGy is carried out at room temperature.After the completion of irradiation, sample is transported back under refrigerated conditions
Laboratory, in -18 DEG C -0 DEG C -- 18 DEG C -0 DEG C is stored under temperature match curing conditions in refrigerator twice, temperature change ibid, 0,7,14,
Sampling is detected at 21,28 days.All samples are in triplicate.
1.2 reagents and instrument
Magnesia, boric acid, hydrochloric acid, methyl red, bromocresol green, ethanol, sodium chloride, plate count agar, trichloroacetic acid, EDTA,
2- thiobarbituricacidα-s, chloroform analyzes pure, purity >=99%, Chemical Reagent Co., Ltd., Sinopharm Group.
PH meter（Plum Teller-support benefit instrument（Shanghai）Co., Ltd）, the type full-automatic azotometers of Kjeltec 2300,
AL104 electronic balances（Mettler-Toledo Instrument (Shanghai) Co., Ltd.）, (the rich news in Shanghai are limited for BJ-2CD superclean benches
Company), GR60DF high-pressure sterilizing pots（Xiamen causes common vetch instrument）, 5804R supercentrifuges（Eppendorf Co, Germany）, at a high speed
Mulser（Shanghai Fu Luluke fluid machinery manufactures have instrument limited company), electron accelerator 10MeV/20kW (Tongfangs
Prestige regards IS1020), Electronic Nose (French Alpha MOS companies).
1.3 test methods
1.3.1 the measure of total plate count
With reference to standard GB/T 4789.2-2010, weigh 5 g rheum officinale fish samples and put the sterile centrifugation for filling 45 mL physiological saline
Guan Zhong, about 1~2 min is mixed with high speed disperser, is made 1：10 sample diluting liquid.1 ml is taken from the dilution, with 9
The SPSS of ml carries out gradient dilution, is made 1：100 sample diluting liquid.By that analogy, therefrom select suitable dilute
Gradient is released, total number of bacteria is determined using dilution-plate method.
1.3.2 TVB-N content is determined
With reference to national marine industry standard SCT 3032-2007, the g of Pseudosciaena crocea meat 10.0 is weighed, add 90 mL perchloric acid
Solution, the min of homogeneous 2 is placed in 250 mL conical flasks, is filtered with qualitative filter paper after standing 30 min, takes the mL of supernatant 5
By kjeldahl apparatus, salt acid consumption is determined.TVB-N is calculated as follows：
1.3.3 TBARS value（TBARS values）Measure
With reference to methods such as Jia Qian, take the meat sample 10g for smashing to pieces and add 50ml, 7.5% trichloroacetic acid（Containing 0.1%EDTA）.Shaking
30min, double-layer filter paper is filtered twice, is taken 5ml supernatants and is added 5ml, the TBA solution of 0.02mol/L, 90 DEG C of water-baths（Boiling water
Bath）Middle insulation 40min, takes out cooling 1h, and 5min is centrifuged（1600r/min）, 5ml chloroforms shaking stratification is added in supernatant
After take supernatant, the colorimetric at 532nm and 600nm respectively records light absorption value, and TBARS values are calculated as follows.TBARS
(mg/100g)=(A532-A600)/155*(1/10)*72.6*100
1.3.4 determined amino acid method
Determined using automatic amino acid analyzer with reference to GB 5009.124-2003, concrete operations：Weigh about 60 mg ~ 70 mg
Sample, precise to 0.1mg adds the mL of 6 mol/L hydrochloric acid solutions 10,24 h is cleared up in 110 DEG C of baking ovens, extremely
Sample is in limpid brownish red.After sample after clearing up is filtered through fast grade filter paper, it is collected in 50 mL volumetric flasks, heartily
Deionized water constant volume.In 10 mL screw sockets bottle, nitrogen blows solution after taking 1 mL constant volumes.Add 5 mL, 0.02 mol/L salt
Acid solution is mixed, and is taken 1 mL mixing liquids and is crossed 0.45 μm of water system filter membrane and is collected in bottle, automatic with L-8900 amino acid
Analyzer is measured.
1.3.5 Electronic Nose is determined
5g Pseudosciaena crocea meats are weighed in 15mL beakers, are sealed, setting detection time is 100s, and scavenging period is 100s,
Detected with Electronic Nose successively.
1.3.6 data analysis
All data are with average value ± variance（Mean±SD）Represent, data carry out data analysis, are used in combination using SPSS 16.0
GarphPad Prism5 are mapped.
2 results and analysis
Influence of 2.1 temperature fluctuations to freezing quality of large yellow croaker
2.1.1 influence of the temperature fluctuation to large yellow croaker total plate count
As shown in Figure 1, occurrence temperature fluctuation can significantly affect the total plate count of large yellow croaker in storage and transport process, test early stage, temperature
When not changing, the total plate count of two groups of large yellow croakers does not have a significant difference, but after being fluctuated at the 12nd day, temperature
The total plate count of fluctuation group apparently higher than all the time be in -18 DEG C of groups because after the 14th day again large yellow croaker is stored in -18 DEG C,
So total plate count gathers way and substantially slows down, and when the 26th day, fish is thawed again, when being stored in 0 DEG C, total plate count
Substantially increase, be much higher than temperature stabilization group.If it is demonstrated experimentally that being in -18 DEG C, at the 28th day, the bacterium of large yellow croaker all the time
The sum that falls still only has 5.14 lgcfug-1, and the large yellow croaker of occurrence temperature fluctuation has reached 6 lgcfug on the 26th day-1,
More than national standard.
2.1.2 influence of the temperature fluctuation to large yellow croaker VBN
VBN is because protein degradation and non-protein nitrogen compound are produced, mainly by ammonia, secondary species
Constituted with amine, during aquatic products storage, it is likely to because the degraded of protein and microbial spoilage cause in muscle
's.Figure it is seen that after reserve temperature fluctuates, large yellow croaker TVB-N content gradually increases, and all the time
Large yellow croaker TVB-N content under the conditions of -18 DEG C does not change substantially, both significant differences.At storage end
Phase, the sample size under the conditions of -18 DEG C is only 12.14 mg N100g-1, and the content of temperature fluctuation group has reached
40.16mg N·100g-1.According to aquatic products freshness national standard[24], the mg N100g of TVBN ＜ 15-1It is 1 grade, TVBN ＜
30 mg N·100g-1It is 2 grades.If thus criterion obtains the temperature in storage and fluctuates, large yellow croaker shelf life
It is only 22.5 days, is arrived up to standard limit within 10 days or so after temperature fluctuation generation, illustrates temperature fluctuation to VBN
Have a great impact.
2.1.3 influence of the temperature fluctuation to large yellow croaker TBARS value
TBARS value is the index for describing fat oxidation degree.Unrighted acid oxidation Decomposition in flesh of fish fat
Produced MDA afterwards（MDA）Red material is generated with thiobarbituric acid reagent reaction, the material has maximum at 532nm
Absworption peak, can be used to represent the lipid oxidation degree of sample.As seen from Figure 3, the extension over time of TBA values and gradually
Increase, and and total plate count, VBN is the same, and when temperature fluctuates, fat oxidation degree is more obvious, advances the speed
Bigger, TBARS value is bigger.When there is first time temperature fluctuation, the TBARS value of large yellow croaker has reached
0.43mg MDA/100g, and still it is not up to this value, only 0.37mg in storage latter stage in -18 DEG C of large yellow croaker all the time
MDA/100g.Illustrate the fluctuation of temperature has apparent facilitation to large yellow croaker fat oxidation.
Influence of the 2.2 different irradiation doses to freezing quality of large yellow croaker
2.2.1 influence of the different irradiation doses to large yellow croaker amino acid
As can be seen from Table 1, by after the treatment of 2kGy and 4kGy electron beam irradiations, in addition to methionine, remaining 15 kinds of amino
Acid content is below the large yellow croaker irradiated without irradiation and through 1kGy, and fall is more than 5%, and such as glutamic acid contains
Amount fall is respectively 24%, 12%.And by the large yellow croaker amino acid of 1kGy irradiation only on lysine content and without spoke
According to have notable difference, fall is 6%.
Below in 16 kinds of amino acid must amino acid mainly have：Valine, threonine, methionine, leucine, different bright ammonia
Acid, tyrosine, phenylalanine and lysine.According to the idealized model of FAO/WHO, the food being of high nutritive value, its protein
Middle essential amino acid accounts for total amino acid（EAA/TAA）40% or so can be reached, it is necessary to the ratio between amino acid and nonessential amino acid
（EAA/NEAA）Then more than 60%.Be can be calculated by the method, the large yellow croaker after 0kGy, 1kGy, 2kGy, 4kGy treatment
EAA/TAA is respectively 44.34%, 43.92%, 44.28%, 44.67%, EAA/NEAA be respectively 79.67%, 78.32%, 79.47%,
80.72%, belong to good protein.Illustrate to comprehend at electron beam irradiation the content for influenceing amino acid to a certain extent, but not
Its nutritive value can be influenceed.
Influence of the different irradiation doses of table 1 to large yellow croaker amino acid
2.2.2 influence of the different irradiation doses to large yellow croaker total plate count
Fig. 4 is under the storage condition of occurrence temperature fluctuation, to be processed to large yellow croaker total plate count with various dose electron beam irradiation
Influence.As seen from the figure, extension over time, the total plate count of the large yellow croaker of all treatment all increased, especially
When fluctuating occurs in temperature, advance the speed and substantially accelerate, but compared to the large yellow croaker without irradiation, irradiated treatment
Total plate count value is increased slow, and dosage is bigger, increases slower.Simultaneously from the graph, it is apparent that in storage
It is initial stage, not irradiated, through 1kGy, 2kGy, 4kGy by the total plate count of large yellow croaker that irradiates significantly lower than without irradiation
Initial value be respectively 4.15 lgcfug-1、3.89 lgcfu·g-1、3.80 lgcfu·g-1、3.74 lgcfu·g-1, and
To latter stage is stored, each group total plate count arrives separately at 6.14 lgcfug-1、5.92 lgcfu·g-1、5.65 lgcfu·g-1
And 5.48 lgcfug-1, although compared with the large yellow croaker for being in -18 DEG C all the time, still there are a certain distance, but radiation treatment
Total plate count can be reduced, extends its shelf life, and irradiation dose is bigger, effect is better.
2.2.3 influence of the different irradiation doses to large yellow croaker TBARS value
As shown in Figure 5, with the extension of storage time, the TBARS value of all groups of large yellow croaker increases.But pass through
The experimental group of irradiation, storage the initial stage can be apparent observe its TBARS value be higher than non-irradiated group, illustrate electricity
Beamlet irradiation accelerates the fat oxidation of large yellow croaker, and it is had a certain impact.But except the large yellow croaker of 4kGy dosage is first from storage
Phase to storage latter stage all especially under conditions of occurrence temperature fluctuation, advances the speed higher than equilibrium temperature apparently higher than other groups
Outside period, the TBARS value of the large yellow croaker after 1kGy and 2kGy dose deliveries except being higher than at the beginning non-irradiated group,
In storage, its value is advanced the speed and relatively delay, to storage latter stage, two groups slightly below non-irradiated group.Illustrate in certain irradiation agent
In the range of amount, electron beam irradiation can slow down due to the increase of the TBARS value that temperature fluctuation and storage time bring
Amplitude.
2.2.4 influence of the different irradiation doses to large yellow croaker Electronic Nose
PCA is that the data that the sensor to being extracted is detected are changed and dimension-reduction treatment, is preserving the basis of main information
On, represented by several principal components, be converted to two-dimentional scatterplot diagram form.In Fig. 6, the 1st principal component（PC1）And Second principal component,
（PC2）Contribution rate sum reached 97.653%, illustrate by various dose electron beam irradiation treatment after sample room gas
Substantially, this 2 principal components can well reflect the multi objective information that sensor is detected to taste difference.
Also explanation irradiation can influence the smell of large yellow croaker simultaneously.This experiment can be seen that by the radar map of each dosage in Fig. 7
The Electronic Nose for using is made up of 6 chemosensing elements, respectively T70/2, PA/2, P30/2, LY2/AA, LY2/gCT.But warp
Cross irradiation large yellow croaker no matter dosage size, from radar map, each sensor to the response of its smell without significant difference,
And compared with non-irradiated large yellow croaker, the response of PA/2 and P30/2 is obviously reduced, this is probably, due to effect of irradiation, to make big
The specific scent that yellow croaker has in itself weakens.
3 discuss
This research preserve large yellow croaker under condition of different temperatures by setting, through total plate count, VBN, thio barbital
The physical and chemical indexs such as acid number detect, although finding all the time in -18 DEG C of large yellow croaker also the extending with the time during storing
Increased, but it is slow to advance the speed, and still preferable quality is maintained in 28 days during testing, but by the big of temperature fluctuation
Yellow croaker, by after alternating temperature twice, each index is increased with faster rate, makes shelf life be reduced to 22 days or so.The result and poplar
Influence result of the temperature and time to ice fresh hairtail quality is consistent during victory equality research Cold Chain Logistics, and this is probably due to temperature
Degree fluctuation causes recrystallization, destroys muscle cell core Fiber structure, the content of lysozyme in meat is increased, simultaneously because warm
The rising of degree, microbial activities is active, accelerates decomposition and the lipid oxidation rates of fish meat protein.And by various dose electricity
Large yellow croaker after the treatment of beamlet irradiation dose, its all kinds of amino acid content there occurs a certain degree of change, but not influence it to seek
Value is supported, still belongs to good protein, this evaluates one with electron beam irradiation in refined research is still nourished to the protein of cold fresh meat
Cause.And electron beam irradiation is to the inhibitory action of large yellow croaker total plate count, the low dosage electron beam irradiation transported with imperial common vetch is protected to clam
The result of study trend of Tibetan is consistent, illustrates that electron beam irradiation can suppress the growth of microorganism, slows down and oppresses putrid and deteriorated, from
At the storage initial stage, just it is significantly lower than without irradiation by the total plate count of the large yellow croaker for irradiating, when preserving for -18 DEG C, micro- life
Thing growth is suppressed, so comparatively there is no substantially change in total plate count, and when latter temperature changes, micro- life
The growth speed of thing can be more relatively slow, so as to by irradiation large yellow croaker total plate count can be less than not irradiated group, but
Growth and the increase of temperature fluctuation number of times over time, the total plate count of 1kGy and 2kGy are advanced the speed and gradually add
Hurry up, final result is expected that not irradiated large yellow croaker group can be approached.Meanwhile, research shows, irradiation can trigger and accelerate free radical
Generation so that in accelerating food fat oxidation.Fat oxidation is the one of the main reasons of high-fat foods quality deterioration.
Lewis etc. points out that the de- bone peeling Fresh Grade Breast of electron beam irradiation can accelerate its fat oxidation.In this experiment, electron beam irradiation is to big
The TBARS value of yellow croaker has a significant impact result unanimously, but the large yellow croaker of 1kGy and 2kGy groups stored by 28 days
Afterwards, its TBARS value is approached with not irradiated group of numerical value, or even is slightly less than non-irradiated group, illustrates necessarily illustrating in certain spoke
According in dosage range, electron beam irradiation can slow down due to the increasing degree of the TBARS value that temperature fluctuation brings, because
This is when radiation treatment is carried out, it should be noted that the selection of irradiation dose.Jia Qian is also once in research electron beam and gamma-ray irradiation to element
The fresh-keeping middle discovery of chicken, electron beam irradiation can slow down increasing degree of the steamed bean curd roll TBARS values with storage time.And in this experiment, electricity
Whether sub- nose can be able to be distinguished by irradiation and irradiation dose to the large yellow croaker after different disposal by PCA dimension-reduction treatment
Difference, but do not find out that the large yellow croaker after irradiation has very big change in the response of each sensor from the radar map of each group
Change, what is had is even less than non-irradiated group, and this may be relevant with the number of sensors of the instrument in experiment and species, it is impossible to will irradiate
Special odor afterwards is detected, to be detected the volatile flavor produced by irradiation, it is necessary to from other method
Further detected.
Although can slightly improve due to the influence that temperature fluctuation brings, due to huge by the large yellow croaker after radiation treatment
The big temperature difference will certainly affect with the quality of large yellow croaker and mouthfeel, so should by the large yellow croaker of temperature fluctuation
Sold as early as possible, it is impossible to preserved for a long time.Seen by this experimental result, if the genuine occurrence temperature ripple in storage and transport process
It is dynamic, only can not well maintain its quality by radiation treatment, it may be considered that carried out by composite fresh-keeping method fresh-keeping, carry certainly
Temperature control in cold chain transportation high and storage is the most key.
4 conclusions
By simulating temperature fluctuation during Cold Chain Logistics, large yellow croaker total plate count, VBN and thio bar are determined
Find that temperature fluctuates in storage, can substantially reduce the shelf life of large yellow croaker than appropriate acid number, and by electron beam spoke
After according to treatment, the harmful effect for thus causing can be alleviated, while comprehensive survey index, it is found that 2kGy and 1kGy is keeping rheum officinale
Fish products matter aspect effect be better than 4kGy, and irradiation dose it is big control total plate count in terms of it is more advantageous.So in reality
In cold chain transportation process, should try one's best and avoid the fluctuation of temperature, and in the irradiation of applying electronic beam, 2kGy is the recommendation for irradiating large yellow croaker
Dosage.
Claims (3)
1. a kind of method that utilization electron beam irradiation ensures quality of large yellow croaker in Cold Chain Logistics transport, it is characterised in that including following
Step：
（1）Sample pretreatment：Screening rheum officinale fish sample, packaging, sabot；
（2）Irradiation：By step（1）In the sample disc equipped with rheum officinale fish sample be placed in and carry out electron beam irradiation treatment at room temperature, electricity
Beamlet irradiation dose is 1-4kGy；
（3）Storage：By step（2）The middle rheum officinale fish sample through electron beam irradiation is placed in -18-0 DEG C--18-0 DEG C alternating temperature twice
Under the conditions of Cold Chain Logistics storage.
2. a kind of utilization electron beam irradiation according to claim 1 ensures the side of quality of large yellow croaker in Cold Chain Logistics transport
Method, it is characterised in that the electron beam irradiation dosage is 1,2 or 4kGy, preferably 2 kGy.
3. the method that a kind of utilization electron beam irradiation according to claim 1 or 2 ensures quality of large yellow croaker in Cold Chain Logistics transport,
It is characterized in that the step（3）The reserve temperature of middle rheum officinale fish sample is -18 DEG C.
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| CN107375965A (en) * | 2017-07-18 | 2017-11-24 | 江门华大生物科技有限公司 | The prepared slices of Chinese crude drugs are without sulphur desinsection method for preserving |
| CN111272697A (en) * | 2020-04-27 | 2020-06-12 | 江苏益客食品集团股份有限公司 | Thiobaturic acid content detection method based on near-infrared hyperspectrum |
| CN112314835A (en) * | 2021-01-06 | 2021-02-05 | 浙江大学 | Sterilizing method and system for inactivating novel coronavirus in cold chain food by ionizing radiation |
| WO2022105346A1 (en) * | 2020-11-19 | 2022-05-27 | 周星 | Novel coronavirus sterilization apparatus and sterilization method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375965A (en) * | 2017-07-18 | 2017-11-24 | 江门华大生物科技有限公司 | The prepared slices of Chinese crude drugs are without sulphur desinsection method for preserving |
| CN107375965B (en) * | 2017-07-18 | 2019-02-15 | 江门华大生物科技有限公司 | The prepared slices of Chinese crude drugs are without sulphur desinsection method for preserving |
| CN111272697A (en) * | 2020-04-27 | 2020-06-12 | 江苏益客食品集团股份有限公司 | Thiobaturic acid content detection method based on near-infrared hyperspectrum |
| WO2022105346A1 (en) * | 2020-11-19 | 2022-05-27 | 周星 | Novel coronavirus sterilization apparatus and sterilization method |
| CN112314835A (en) * | 2021-01-06 | 2021-02-05 | 浙江大学 | Sterilizing method and system for inactivating novel coronavirus in cold chain food by ionizing radiation |
| CN112314835B (en) * | 2021-01-06 | 2021-04-06 | 浙江大学 | Sterilizing method and system for inactivating novel coronavirus in cold chain food by ionizing radiation |
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