CN106706927A - Thyroglobulin enzyme linked immunosorbent diagnostic kit - Google Patents
Thyroglobulin enzyme linked immunosorbent diagnostic kit Download PDFInfo
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- CN106706927A CN106706927A CN201710025001.5A CN201710025001A CN106706927A CN 106706927 A CN106706927 A CN 106706927A CN 201710025001 A CN201710025001 A CN 201710025001A CN 106706927 A CN106706927 A CN 106706927A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/046—Thyroid disorders
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Abstract
The invention belongs to the technical field of medical biology, and particularly relates to a thyroglobulin enzyme linked immunosorbent diagnostic kit, which is formed by a thyroglobulin polyclonal antibody-coated plate, a contrast buffer solution, an enzyme labeling solution, washing liquid, a developing solution, a substrate solution, a thyroglobulin standard substance and a stop solution. The thyroglobulin enzyme linked immunosorbent diagnostic kit has high sensitivity, high specificity, high accuracy, and favorable stability, the expiry date of the kit at the room temperature is 1 year, the expiry date of the kit at the temperature of 4 DEG C is 3 years, and the storage life of the kit is remarkably prolonged.
Description
Technical field
The invention belongs to technical field of pharmaceutical biotechnology, and in particular to a kind of thyroglobulin elisa diagnostic kit
Box.
Background technology
Thyroid disease is common disease, frequently-occurring disease in China.Thyroglobulin (TG) is distinctive a kind of anti-thyroid gland
Original, with important clinical meaning, can all have in various Patients With Various Thyroid Disorders serum and occur TG exceptions to some extent.Face
By determining the content of TG on bed, contribute to the diagnosis of thyroid disease.
TG is the 660ku glycoprotein of thyroid follicular epithelial cells secretion, and each TG there are about 2 thyroxine (T4) and 0.5
Triiodo thryonine (T3) molecule, is stored in follicular cavity.Lysosomal hydrolysis TG surface T4, T3 simultaneously is allowed to be released into blood, together
The TG of Shi Shaoliang is also released into blood, and part TG is secreted into blood through lymphatics of thyroid gland.TG in blood circulation is thin by the macrophage of liver
Born of the same parents remove.More common in the malignant tumour at thyroid gland position, such as TG is in follicular carcinoma of thyroid, thyroid gland breast for the change of serum TG
Head cancer and anaplasia cancer all may occur in which different degrees of rising；And medullary carcinoma of thyroid gland blood TG can normally or reduce.The former is main
Because thyroid destruction and tumor tissues secrete a certain amount of TG, and TG is raised in causing blood；The tumor tissues of the latter come
Parafollicular cells of thyroid gland, rather than Thyroid follicular epithelial cell are come from, therefore its serum TG is not raised.Hyperthyroidism, thyroid gland
The thyroid diseases such as knurl, subacute thyroiditis and chronic lymphocytic thyroiditis all may occur in which TG level liters in blood
It is high.For from the angle of detection, the method for being clinically used to determine TG at present mainly has radio immunoassay, enzyme linked immunological
Method (ELISA), chemiluminescence immunoassay etc..
Thyroglobulin (TG) of the past with radio immunoassay as representative determines kit and is limited by methodology,
Its sensitivity and antijamming capability wretched insufficiency, the drawbacks of have very big, substantially withdraw from the market；Chemiluminescence immune assay
The complex operation of method, the term of validity is short, is unfavorable for popularization and application；ELISA method as quantitative and semi-quantitative detection method, antigen,
The association reaction of antibody is carried out on solid phase (elisa plate reacting hole) surface, and its sensitivity, specificity are preferable, and economical
Material benefit, thus be apply most wide, most methods.But in enzyme-linked immunologic diagnosis kit when using, the stabilization of kit
Property be limit product the marketization key.The thyroglobulin enzyme-linked immunologic diagnosis kit of present market different manufacturers production
Generally existing the problems such as storage life is short, stability is poor, strongly limit thyroglobulin enzyme-linked immunologic diagnosis kit and exists
Clinically promotion and application.The patent and product of the existing multinomial relevant stabilizer of foreign countries come out, and the country is steady in diagnosis examination box
Agent aspect is determined still in the exploratory stage, and Study of Stabilizer turns into the technology bottle that China's enzyme-linked immunologic diagnosis kit research and development must pull against
Neck.At present, for stabilizers such as sucrose, trehalose, lactose, gelatin, glycerine, sorbierite, sodium glutamate, polyethylene glycol
Research.
The content of the invention
In order to the storage life for solving generally existing in existing thyroglobulin enzyme-linked immunologic diagnosis kit is short, stability
The problems such as difference, the invention provides a kind of thyroglobulin enzyme-linked immunologic diagnosis kit, the kit has good steady
Qualitative, the term of validity is 1 year at ambient temperature, and the term of validity significantly improved the preservation of kit up to 3 years under the conditions of 4 DEG C
Phase.
The thyroglobulin enzyme-linked immunologic diagnosis kit that the present invention is provided, by thyroglobulin polyclonal antibody bag
By plate, control buffer solution, enzyme marking fluid, cleaning solution, nitrite ion, substrate solution, thyroglobulin standard items and terminate liquid composition；
Wherein, the preparation process of the thyroglobulin polyclonal antibody coating plate is：By the thyroid gland ball of coating
After protein polyclone antibody is diluted with the carbonate buffer solution liquid of 0.05mol/L pH9.0, by 0.1mL/ holes coating to microwell plate
It is interior, after 4~8 DEG C of absorption 24~26 hours, with the phosphate buffer board-washing of 0.05mol/L pH9.0, then with confining liquid 4
~8 DEG C are closed 18~20 hours, and the unnecessary confining liquid of reject is obtained thyroglobulin Anti-TNF-α after vacuum dried treatment
Body is coated with plate；
The enzyme marking fluid be the horseradish peroxidase-labeled containing 0.5mg/L thyroglobulin monoclonal antibody,
The 15mmol/L pH8.0 phosphate buffers of the polyethylene glycol -200 of 0.1~0.6mg/L and the shitosan of 1~3mg/L.
Further, the control buffer solution is the phosphate buffer of 15mmol/L pH7.4.
Further, the cleaning solution is the phosphate buffer of the 15mmol/L pH7.4 of the Tween-20 containing 0.5g/L.
Further, the nitrite ion is the methanol solution of tetramethyl benzidine, and concentration is 0.1mg/mL.
Further, the terminate liquid is 3mol/L H2SO4Solution.
Further, the substrate solution is 3% hydrogenperoxide steam generator that pH7.4 phosphoric acid-citrate buffer solution is prepared, and
The Sodium Acid Pyrophosphate of 0.1mg/L in solution.
Further, the confining liquid is the egg of the sucrose containing 3~5g/L, the rhamnolipid of 0.5~2g/L and 1.5g/L
The phosphate buffer of the 20mmol/L pH9.0 of pure albumen.
Further, the thyroglobulin standard items are that the thyroglobulin for taking respective amount is dissolved in and contains 3g/L
The phosphate buffer of the 20mmol/L pH9.0 of chicken ovalbumin, the concentration of thyroglobulin standard items is followed successively by 1.0 μ
G/L, 2.5 μ g/L, 5.0 μ g/L, 10 μ g/L, 25 μ g/L, 50 μ g/L, 100 μ g/L and 200 μ g/L.
The Cleaning Principle of thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention：
This kit is in microwell plate endoperidium thyroglobulin polyclonal antibody, if there is thyroid gland ball egg in sample to be tested
In vain can be in combination, then the thyroglobulin monoclonal antibody reaction marked with enzyme, " Ag-Ab-enzyme is combined for formation
Thing ", enzymatic chromogenic reagent can determine whether the presence or absence of thyroglobulin, and determine thyroid gland ball egg according to colour developing degree
White content.In addition, kit of the present invention has used the confining liquid containing sucrose and rhamnolipid during envelope antigen,
Polyethylene glycol -200 and shitosan are with the addition of in enzyme marking fluid.The combination of sucrose and rhamnolipid, and polyethylene glycol -200
Combination with shitosan plays a very good protection as stabilizer to envelope antigen, enzyme labelled antibody, make envelope antigen,
Affinity, immunocompetence, sensitivity of enzyme labelled antibody etc. are not reduced, so as to significantly improve the storage life of kit of the present invention.
Therefore, compared with prior art, advantage of the invention is that：
The sensitivity of thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention is high, high specificity, the degree of accuracy are high, and tool
There is good stability, the term of validity is 1 year at ambient temperature, and the term of validity significantly improved examination up to 3 years under the conditions of 4 DEG C
The storage life of agent box, is conducive to the promotion and application clinically of thyroglobulin enzyme-linked immunologic diagnosis kit.
Specific embodiment
The present invention will further be described in detail below.It is pointed out that following explanation is only to application claims
Protection it is technical scheme for example, not to any limitation of these technical schemes.Protection scope of the present invention is with appended
The content that claims are recorded is defined.
In the present invention, thyroglobulin, thyroglobulin polyclonal antibody and horseradish peroxidase-labeled
Thyroglobulin monoclonal antibody can be obtained by commercial channel.Rhamnolipid (Beige powder, 45~60%, pH5~
6) purchased from Daqing Vertex Chemical Co., Ltd..This biotechnology is limited purchased from Xi'an south for shitosan (active principle content 99%)
Company.
In addition, method of the unreceipted specific experiment condition with operating in the following example, according to this area conventional method
Carry out.
Embodiment 1, thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention
The present embodiment kit is by thyroglobulin polyclonal antibody coating plate, control buffer solution, enzyme marking fluid, washing
Liquid, nitrite ion, substrate solution, thyroglobulin standard items and terminate liquid composition；
Wherein, the preparation process of the thyroglobulin polyclonal antibody coating plate is：By the thyroid gland ball of coating
After protein polyclone antibody is diluted with the carbonate buffer solution liquid of 0.05mol/L pH9.0, by 0.1mL/ holes coating to microwell plate
It is interior, after 4 DEG C of absorption 24 hours, with the phosphate buffer board-washing of 0.05mol/L pH9.0, then closed at 4 DEG C with confining liquid
18 hours, the unnecessary confining liquid of reject was obtained thyroglobulin polyclonal antibody coating plate after vacuum dried treatment；
The enzyme marking fluid be the horseradish peroxidase-labeled containing 0.5mg/L thyroglobulin monoclonal antibody,
The 15mmol/L pH8.0 phosphate buffers of the polyethylene glycol -200 of 0.1mg/L and the shitosan of 1mg/L.
The control buffer solution is the phosphate buffer of 15mmol/L pH7.4.
The cleaning solution is the phosphate buffer of the 15mmol/L pH7.4 of the Tween-20 containing 0.5g/L.
The nitrite ion is the methanol solution of tetramethyl benzidine, and concentration is 0.1mg/mL.
The terminate liquid is 3mol/L H2SO4Solution.
The substrate solution is 3% hydrogenperoxide steam generator that pH7.4 phosphoric acid-citrate buffer solution is prepared, and in solution
The Sodium Acid Pyrophosphate of 0.1mg/L.
The confining liquid is the egg white albumin of the sucrose containing 3g/L, the rhamnolipid of 0.5g/L and 1.5g/L
The phosphate buffer of 20mmol/L pH9.0.
The thyroglobulin standard items be take respective amount thyroglobulin be dissolved in it is pure containing 3g/L ovum gallinaceums
The phosphate buffer of the 20mmol/L pH9.0 of albumen, the concentration of thyroglobulin standard items is followed successively by 1.0 μ g/L, 2.5 μ
G/L, 5.0 μ g/L, 10 μ g/L, 25 μ g/L, 50 μ g/L, 100 μ g/L and 200 μ g/L.
Embodiment 2, thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention
The present embodiment kit is by thyroglobulin polyclonal antibody coating plate, control buffer solution, enzyme marking fluid, washing
Liquid, nitrite ion, substrate solution, thyroglobulin standard items and terminate liquid composition；
Wherein, the preparation process of the thyroglobulin polyclonal antibody coating plate is：By the thyroid gland ball of coating
After protein polyclone antibody is diluted with the carbonate buffer solution liquid of 0.05mol/L pH9.0, by 0.1mL/ holes coating to microwell plate
It is interior, after 8 DEG C of absorption 26 hours, with the phosphate buffer board-washing of 0.05mol/L pH9.0, then closed at 8 DEG C with confining liquid
20 hours, the unnecessary confining liquid of reject was obtained thyroglobulin polyclonal antibody coating plate after vacuum dried treatment；
The enzyme marking fluid be the horseradish peroxidase-labeled containing 0.5mg/L thyroglobulin monoclonal antibody,
The 15mmol/L pH8.0 phosphate buffers of the polyethylene glycol -200 of 0.6mg/L and the shitosan of 3mg/L.
The control buffer solution is the phosphate buffer of 15mmol/L pH7.4.
The cleaning solution is the phosphate buffer of the 15mmol/L pH7.4 of the Tween-20 containing 0.5g/L.
The nitrite ion is the methanol solution of tetramethyl benzidine, and concentration is 0.1mg/mL.
The terminate liquid is 3mol/L H2SO4Solution.
The substrate solution is 3% hydrogenperoxide steam generator that pH7.4 phosphoric acid-citrate buffer solution is prepared, and in solution
The Sodium Acid Pyrophosphate of 0.1mg/L.
The confining liquid is the 20mmol/L of the egg white albumin of the sucrose containing 5g/L, the rhamnolipid of 2g/L and 1.5g/L
The phosphate buffer of pH9.0.
The thyroglobulin standard items be take respective amount thyroglobulin be dissolved in it is pure containing 3g/L ovum gallinaceums
The phosphate buffer of the 20mmol/L pH9.0 of albumen, the concentration of thyroglobulin standard items is followed successively by 1.0 μ g/L, 2.5 μ
G/L, 5.0 μ g/L, 10 μ g/L, 25 μ g/L, 50 μ g/L, 100 μ g/L and 200 μ g/L.
Embodiment 3, thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention
The present embodiment kit is by thyroglobulin polyclonal antibody coating plate, control buffer solution, enzyme marking fluid, washing
Liquid, nitrite ion, substrate solution, thyroglobulin standard items and terminate liquid composition；
Wherein, the preparation process of the thyroglobulin polyclonal antibody coating plate is：By the thyroid gland ball of coating
After protein polyclone antibody is diluted with the carbonate buffer solution liquid of 0.05mol/L pH9.0, by 0.1mL/ holes coating to microwell plate
It is interior, after 6 DEG C of absorption 24 hours, with the phosphate buffer board-washing of 0.05mol/L pH9.0, then closed at 6 DEG C with confining liquid
18 hours, the unnecessary confining liquid of reject was obtained thyroglobulin polyclonal antibody coating plate after vacuum dried treatment；
The enzyme marking fluid be the horseradish peroxidase-labeled containing 0.5mg/L thyroglobulin monoclonal antibody,
The 15mmol/L pH8.0 phosphate buffers of the polyethylene glycol -200 of 0.4mg/L and the shitosan of 2mg/L.
The control buffer solution is the phosphate buffer of 15mmol/L pH7.4.
The cleaning solution is the phosphate buffer of the 15mmol/L pH7.4 of the Tween-20 containing 0.5g/L.
The nitrite ion is the methanol solution of tetramethyl benzidine, and concentration is 0.1mg/mL.
The terminate liquid is 3mol/L H2SO4Solution.
The substrate solution is 3% hydrogenperoxide steam generator that pH7.4 phosphoric acid-citrate buffer solution is prepared, and in solution
The Sodium Acid Pyrophosphate of 0.1mg/L.
The confining liquid is the 20mmol/L of the egg white albumin of the sucrose containing 4g/L, the rhamnolipid of 1g/L and 1.5g/L
The phosphate buffer of pH9.0.
The thyroglobulin standard items be take respective amount thyroglobulin be dissolved in it is pure containing 3g/L ovum gallinaceums
The phosphate buffer of the 20mmol/L pH9.0 of albumen, the concentration of thyroglobulin standard items is followed successively by 1.0 μ g/L, 2.5 μ
G/L, 5.0 μ g/L, 10 μ g/L, 25 μ g/L, 50 μ g/L, 100 μ g/L and 200 μ g/L.
Comparative example 1
Compared with Example 3, this comparative example is differed only in：Without rhamnolipid in the confining liquid, sucrose contains
Amount is changed to 5g/L by 4g/L.
Comparative example 2
Compared with Example 3, this comparative example is differed only in：Without sucrose in the confining liquid, rhamnolipid contains
Amount is changed to 5g/L by 1g/L.
Comparative example 3
Compared with Example 3, this comparative example is differed only in：Without shitosan in the enzyme marking fluid, poly- second two
The content of alcohol -200 is changed to 2.4mg/L by 0.4mg/L.
Comparative example 4
Compared with Example 3, this comparative example is differed only in：The content of shitosan is by 2mg/L in the enzyme marking fluid
It is changed to 4mg/L.
The detection of test example, thyroglobulin enzyme-linked immunologic diagnosis kit of the present invention：
1. the operating process of detection method：
(1) reagent prepares：Kit is balanced to room temperature；
(2) it is loaded：The thyroglobulin polyclonal antibody coated slab of requirement is fixed on grillage, is sequentially added
Enter 50 μ l test serums samples or thyroglobulin standard items；Experiment every time sets blank (control buffer solution)；Concussion is mixed
It is even, shrouding, 37 DEG C incubate 50 minutes, with cleaning solution board-washing 5 times, pat dry.
(3) it is enzyme-added：The μ l of enzyme marking fluid 100 are added per hole, concussion is mixed, shrouding, 37 DEG C incubate 50 minutes, are washed with cleaning solution
Plate 5 times, pats dry.
(4) develop the color readings：Substrate solution, each 50 μ l of nitrite ion are added per hole, is mixed, shrouding, put 37 DEG C of lucifuges and develop the color 20 points
Clock.Add the μ l of terminate liquid 50 per hole, mix, result is completed in 10 minutes and is determined.Each hole is determined under 450nm wavelength with ELIASA
OD values are simultaneously recorded.
It is logarithm abscissa with thyroglobulin content, with absorbance as ordinate, mark is drawn on logarithmic paper
Directrix curve, checks in the thyroglobulin content of each testing sample from standard curve.
2. performance indications：
The μ of μ g/L to 200 g/L of thyroglobulin standard curve range 1.0, corresponding absorbance is that 0.3~1.5. is curved
Performance.Blank absorbency is less than 0.2.
(1) sensitivity for analysis：It is respectively 0.25,0.5,1.0,2.0,4.0,8.0 μ g/L's to thyroglobulin content
Standard items and blank are respectively determined 12 times, obtain the average and standard deviation of each group.3 times of zero standards are added with blank average
Poor absorbance correspondence thyroglobulin amount is the detection lower bound of this law, is as a result 0.10 μ g/L.
The thyroid gland ball egg that the group with the CV of the replication value of a certain thyroglobulin closest to 20% has
White amount is the Functional Sensitivity of this law, you can the minimum of quantifying reporter, is defined as 1.0 μ g/L.
(2) precision：2 standard items are taken, concentration is 20 μ g/L and 100 μ g/L, and each standard items determines 8 again simultaneously
Pipe, variation within batch coefficient is 7~9%.2 standard items are taken, concentration is 20 μ g/L and 100 μ g/L, and each standard items determines 8 simultaneously
Secondary, interassay coefficient of variation is respectively 11~12%.
(3) accuracy：2 standard items are taken, concentration is 100 μ g/L and 200 μ g/L, respectively adds a certain amount of thyroid gland ball egg
White standard items, determine the rate of recovery of thyroglobulin between 93~106%, and average recovery rate is 101.6%.
(4) stability：
After kit prepared by embodiment 1~3 and comparative example 1~4 is placed 6 months and 12 months respectively at 20 DEG C, survey
Determine the sensitivity of kit, and data are carried out with regression analysis, calculate R2Value.Result see the table below.
As seen from the above table：
1) kit of the embodiment of the present invention 1~3 has good stability, clever after being placed 6 months and 12 months respectively at 20 DEG C
Sensitivity is constant, and linear good.In addition, the kit of the embodiment of the present invention 1~3 is preserved 36 months at 4 DEG C, its sensitivity and line
Property it is good, with the firm kit for preparing without significant difference.
2) sensitivity of the kit of comparative example 1~3 is decreased obviously after being placed 6 months and 12 months respectively at 20 DEG C, and line
Property is not good；And the sensitivity of the kit of comparative example 4 has only declined, but it is not linear good.
Present invention merely illustrates some claimed specific embodiments, one of them or more skill
Described technical characteristic can be combined with arbitrary one or more technical schemes in art scheme, these are combined and obtain
Technical scheme also in the application protection domain, technical scheme is disclosed in the present invention just as obtained from these are combined
It is specific in content to record the same.
Claims (8)
1. a kind of thyroglobulin enzyme-linked immunologic diagnosis kit, it is characterised in that by thyroglobulin polyclonal antibody
Coating plate, control buffer solution, enzyme marking fluid, cleaning solution, nitrite ion, substrate solution, thyroglobulin standard items and terminate liquid group
Into；
Wherein, the preparation process of the thyroglobulin polyclonal antibody coating plate is：By the thyroglobulin of coating
After polyclonal antibody is diluted with the carbonate buffer solution liquid of 0.05mol/L pH9.0, it is coated with microwell plate by 0.1mL/ holes,
After 4~8 DEG C of absorption 24~26 hours, with the phosphate buffer board-washing of 0.05mol/L pH9.0, then with confining liquid at 4~8 DEG C
Closing 18~20 hours, the unnecessary confining liquid of reject is obtained thyroglobulin polyclonal antibody bag after vacuum dried treatment
By plate；
The enzyme marking fluid be the thyroglobulin monoclonal antibody of the horseradish peroxidase-labeled containing 0.5mg/L, 0.1~
The 15mmol/L pH8.0 phosphate buffers of the polyethylene glycol -200 of 0.6mg/L and the shitosan of 1~3mg/L.
2. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the control buffering
Liquid is the phosphate buffer of 15mmol/L pH7.4.
3. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the cleaning solution is
The phosphate buffer of the 15mmol/L pH7.4 of the Tween-20 containing 0.5g/L.
4. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the nitrite ion is
The methanol solution of tetramethyl benzidine, concentration is 0.1mg/mL.
5. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the terminate liquid is
3mol/L H2SO4Solution.
6. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the substrate solution is
3% hydrogenperoxide steam generator that pH7.4 phosphoric acid-citrate buffer solution is prepared, and in solution 0.1mg/L Sodium Acid Pyrophosphate.
7. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the confining liquid is
The phosphoric acid of the 20mmol/L pH9.0 of the rhamnolipid of sucrose, 0.5~2g/L containing 3~5g/L and the egg white albumin of 1.5g/L
Salt buffer.
8. thyroglobulin enzyme-linked immunologic diagnosis kit as claimed in claim 1, it is characterised in that the thyroid gland ball
Protein standard substance is that the thyroglobulin for taking respective amount is dissolved in the 20mmol/L pH9.0 containing 3g/L chicken ovalbumins
Phosphate buffer, the concentration of thyroglobulin standard items be followed successively by 1.0 μ g/L, 2.5 μ g/L, 5.0 μ g/L, 10 μ g/L,
25 μ g/L, 50 μ g/L, 100 μ g/L and 200 μ g/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710025001.5A CN106706927A (en) | 2017-01-13 | 2017-01-13 | Thyroglobulin enzyme linked immunosorbent diagnostic kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710025001.5A CN106706927A (en) | 2017-01-13 | 2017-01-13 | Thyroglobulin enzyme linked immunosorbent diagnostic kit |
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| CN106706927A true CN106706927A (en) | 2017-05-24 |
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| CN201710025001.5A Pending CN106706927A (en) | 2017-01-13 | 2017-01-13 | Thyroglobulin enzyme linked immunosorbent diagnostic kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107870234A (en) * | 2017-10-19 | 2018-04-03 | 施康培医疗科技（武汉）有限公司 | A kind of test strips for detecting thyroglobulin concentration and preparation method thereof |
| CN114112957A (en) * | 2021-11-26 | 2022-03-01 | 浙江夸克生物科技有限公司 | Adiponectin determination kit and application thereof |
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| CN114112957A (en) * | 2021-11-26 | 2022-03-01 | 浙江夸克生物科技有限公司 | Adiponectin determination kit and application thereof |
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Application publication date: 20170524 |