CN106706617A - Detection method and kit for detecting furazolidone metabolite in animal tissues - Google Patents
Detection method and kit for detecting furazolidone metabolite in animal tissues Download PDFInfo
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- CN106706617A CN106706617A CN201611114150.0A CN201611114150A CN106706617A CN 106706617 A CN106706617 A CN 106706617A CN 201611114150 A CN201611114150 A CN 201611114150A CN 106706617 A CN106706617 A CN 106706617A
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention discloses a detection method and a kit for detecting a furazolidone metabolite in animal tissues, and relates to the technical field of detection of the furazolidone metabolite. The kit for detecting the furazolidone metabolite in the animal tissues comprises a box body; a protective film, a reaction film and a water absorption pad are sequentially installed in the box body from top to bottom; the box body is internally filled with ethanol and a sodium hydroxide solution. The detection method and the kit for detecting the furazolidone metabolite in the animal tissues achieve the effect of detecting whether the animal tissues contain the furazolidone metabolite or not by using the ethanol, a sodium chloride solution, dimethyl formamide, sodium nitroprusside and the sodium hydroxide solution, thus being convenient to use by a user, facilitating the furazolidone metabolite detection of detection personnel, and enabling the method to be conveniently used.
Description
Technical field
The present invention relates to the detection technique field of Furaxone metabolite, furazolidone generation in specially a kind of animal tissue
Thank to the detection method and kit of thing.
Background technology
Furazolidone, former name claims, furazolidone, is a kind of Nitrofuran antibiotics, can be used to treat bacterium and protozoon
The gastrointestinal distress such as the dysentery, enteritis, the gastric ulcer that cause, furazolidone is broad spectrum antibiotic, to common Gram-negative bacteria
There is inhibitory action with positive bacteria, furazolidone is classified as the medicine for prohibitting the use of for The Ministry of Agriculture of the People's Republic of China, MOA, must not be dynamic
Detected in physical property food, prohibit itrofurans, including furazolidone within 2002, the use in animal food,
Furaxone metabolite is itrofurans antimicrobial, there is certain antibacterial action, including sramana to Grain-positive and negative bacterium
Pseudomonas, Shigella, Escherichia coli, Klebsiella Pneumoniae, Enterobacter, S. aureus L-forms, enterococcus faecalis, micrococcus scarlatinae, suddenly
Random vibrios, Campylobacter, Bacteroides etc., also active to trichmonad, giardia lamblia stiles under finite concentration, its mechanism of action
To disturb bacterial oxidation reductase so as to block the eubolism of bacterium.
Kit is the box for holding the chemical reagent such as detection chemical composition, medicament residue, viral species, general doctor
Institute, pharmacy corporation are used, and in the detection of Furaxone metabolite in being related to animal tissue, will be related to furan in animal tissue
Mutter oxazolone metabolin detection method and kit use.
But it is difficult to detect Furaxone metabolite in animal tissue in the market, so as to the person of being not convenient to use
Operation, whether contain Furaxone metabolite in animal tissue so as to be difficult to clear and definite easily detecting, it is also difficult to detect
The content of Furaxone metabolite.
The content of the invention
（One）The technical problem of solution
In view of the shortcomings of the prior art, the detection method the invention provides Furaxone metabolite in a kind of animal tissue and examination
Agent box, Furaxone metabolite is not easy to detection in solving the problems, such as animal tissue.
（Two）Technical scheme
To realize object above, the present invention is achieved by the following technical programs：Furazolidone metabolite in a kind of animal tissue
The detection method of thing, comprises the following steps：
A1：Furaxone metabolite is differentiated；
A2：Furaxone metabolite is checked；
A3：Furaxone metabolite content after detection is measured.
Preferably, described A1 is comprised the following steps：
S1：Furaxone metabolite about 20mg is taken to be placed in box body.
S2：Plus ethanol 5ml~5.5ml and sodium hydroxide solution 3ml, that is, show red；
S3：After adding dimethylformamide 0.1ml~0.15ml dissolvings, the 5ml~6ml that adds water, sodium nitroprusside test solution 1ml
~1.6ml and sodium hydroxide test solution 1ml~1.3ml, is shaken up, and places 2 minutes, and olive-green is shown at the beginning of solution, is faded to blackish green；
S4：Furaxone metabolite 1g is taken, add water 100ml~150ml, shaken 15 minutes, filtering；
S5：Furaxone metabolite about 50mg is taken, it is accurately weighed, put in 10ml measuring bottles, dimethylformamide 5ml~8ml is added,
Tepor makes dissolving in putting water-bath, lets cool, and scale is diluted to acetone, shakes up, and as need testing solution, separately takes 5- nitryl furfurals two
Acetic acid esters reference substance is appropriate, with dimethylformamide-acetone (1:1) solution is quantitatively made the solution containing 50 μ g in every 1ml, as
Reference substance solution, draws each 20 μ l~25 μ l of above two solution, puts respectively on same silica gel g thin-layer plate, with toluene-Isosorbide-5-Nitrae
Dioxane (95:5) it is solvent, launches, dry, in 105 DEG C of dryings 5 minutes, spray (took phenylhydrazine hydrochloride with phenylhydrazine hydrochloride solution
0.75g~0.9g, plus ethanol 10ml~13ml makes dissolving, is diluted with water to 50ml~55ml, with activated carbon decolorizing, takes filtrate,
Plus hydrochloric acid 25ml, add water to 200ml), heated 5 minutes at 105 DEG C, need testing solution is molten with reference substance as shown the impurity spot
Liquid principal spot compares.
Preferably, described A2 includes that step is as follows：
S6：Lucifuge is operated, and takes Furaxone metabolite about 20mg, accurately weighed, is put in 250ml measuring bottles, adds dimethyl formyl
Amine 40ml~43ml, shaking makes dissolving, is diluted with water to scale, shakes up, and precision measures 10ml, dilute with water in putting 100ml measuring bottles
Release to scale, shake up, used as need testing solution, the mensuration absorbance at the wavelength of 367nm separately takes furazolidone reference substance and fits
Amount, it is accurately weighed, it is measured in the same method, calculate, obtain final product.
Preferably, described A3 includes that step is as follows:
S7：Need testing solution compares, if being deeper than 1.0%, animal groups as shown the impurity spot with reference substance solution principal spot
Contain Furaxone metabolite in knitting.
Present invention also offers Furaxone metabolite kit in a kind of animal tissue, including box body, the box body
Inside is installed with diaphragm, reaction film and adsorptive pads successively from top to bottom.
Preferably, the inside of the box body is placed with ethanol and sodium hydroxide solution.
Preferably, the adsorptive pads are made up of the different cotton pad in two-layer aperture.
（Three）Beneficial effect
The invention provides the detection method and kit of Furaxone metabolite in a kind of animal tissue.Possesses following beneficial effect
Really：
（1）, in the animal tissue Furaxone metabolite detection method and kit, by ethanol, sodium chloride solution, two
Whether the application method of NMF, sodium nitroprusside and sodium hydroxide test solution, reached and contain in detection animal tissue
There is the effect of Furaxone metabolite, consequently facilitating the use of user, has been also convenient for testing staff to Furaxone metabolite
Detection, be also convenient for the use of the method.
（2）, in the animal tissue Furaxone metabolite detection method and kit, by the medicine reaction time and
The method of the control of temperature, has reached the effect for making fully to be reacted between medicine, so that the speed of detection is more quick, also carries
The efficiency of detection high, facilitates the use of tester, has been also convenient for the use of the method.
Brief description of the drawings
Fig. 1 is the structural representation of Furaxone metabolite kit in animal tissue of the present invention.
In figure：1 box body, 2 diaphragms, 3 reaction films, 4 adsorptive pads.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Embodiment one
The detection method of Furaxone metabolite, comprises the following steps in a kind of animal tissue：
S1：Furaxone metabolite about 20mg is taken to be placed in box body；
S2：Plus ethanol 5ml and sodium hydroxide solution 3ml, that is, show red；
S3：After adding dimethylformamide 0.1ml dissolvings, the 5ml that adds water, sodium nitroprusside test solution 1ml and NaOH are tried
Liquid 1ml, is shaken up, and places 2 minutes, and olive-green is shown at the beginning of solution, is faded to blackish green；
S4：Furaxone metabolite 1g is taken, add water 100ml, shaken 15 minutes, filtering；
S5：Furaxone metabolite about 50mg is taken, it is accurately weighed, put in 10ml measuring bottles, dimethylformamide 5ml is added, put water
Tepor makes dissolving in bath, lets cool, and scale is diluted to acetone, shakes up, and as need testing solution, separately takes 5- nitryl furfural oxalic acid
Ester reference substance is appropriate, with dimethylformamide-acetone (1:1) solution is quantitatively made the solution containing 50 μ g in every 1ml, used as control
Product solution, draws each 20 μ l of above two solution, puts respectively on same silica gel g thin-layer plate, with toluene-Isosorbide-5-Nitrae dioxane
(95:5) be solvent, launch, dry, in 105 DEG C of dryings 5 minutes, spray with phenylhydrazine hydrochloride solution (take phenylhydrazine hydrochloride 0.75g, plus
Ethanol 10ml makes dissolving, is diluted with water to 50ml, with activated carbon decolorizing, takes filtrate, plus hydrochloric acid 25ml, adds water to 200ml),
105 DEG C are heated 5 minutes, and need testing solution compares as shown the impurity spot with reference substance solution principal spot；
S6：Lucifuge is operated, and takes Furaxone metabolite about 20mg, accurately weighed, is put in 250ml measuring bottles, adds dimethyl formyl
Amine 40ml, shaking makes dissolving, is diluted with water to scale, shakes up, and precision measures 10ml, puts in 100ml measuring bottles, is diluted with water to quarter
Degree, shakes up, and used as need testing solution, the mensuration absorbance at the wavelength of 367nm separately takes furazolidone reference substance in right amount, accurate
It is weighed, it is measured in the same method, calculate, obtain final product；
S7：Need testing solution compares, if being deeper than 1.0%, animal groups as shown the impurity spot with reference substance solution principal spot
Contain Furaxone metabolite in knitting.
Embodiment two
The detection method of Furaxone metabolite, comprises the following steps in a kind of animal tissue：
S1：Furaxone metabolite about 20mg is taken to be placed in box body；
S2：Plus ethanol 5.3ml and sodium hydroxide solution 3ml, that is, show red；
S3：After adding dimethylformamide 0.12ml dissolvings, the 5.5ml that adds water, sodium nitroprusside test solution 1.3ml and hydroxide
Sodium test solution 1.2ml, is shaken up, and places 2 minutes, and olive-green is shown at the beginning of solution, is faded to blackish green；
S4：Furaxone metabolite 1g is taken, add water 125ml, shaken 15 minutes, filtering；
S5：Furaxone metabolite about 50mg is taken, it is accurately weighed, put in 10ml measuring bottles, dimethylformamide 6.5ml is added, put
Tepor makes dissolving in water-bath, lets cool, and scale is diluted to acetone, shakes up, and as need testing solution, separately takes 5- nitryl furfural diethyls
Acid esters reference substance is appropriate, with dimethylformamide-acetone (1:1) solution is quantitatively made the solution containing 50 μ g in every 1ml, used as right
According to product solution, each 22.5 μ l of above two solution are drawn, put respectively on same silica gel g thin-layer plate, with toluene-Isosorbide-5-Nitrae dioxy six
Ring (95:5) be solvent, launch, dry, in 105 DEG C of dryings 5 minutes, spray with phenylhydrazine hydrochloride solution (take phenylhydrazine hydrochloride 0.8g,
Plus ethanol 11ml makes dissolving, is diluted with water to 52.5ml, with activated carbon decolorizing, filtrate, plus hydrochloric acid 25ml are taken, added water to
200ml), heated 5 minutes at 105 DEG C, need testing solution compares as shown the impurity spot with reference substance solution principal spot；
S6：Lucifuge is operated, and takes Furaxone metabolite about 20mg, accurately weighed, is put in 250ml measuring bottles, adds dimethyl formyl
Amine 41ml, shaking makes dissolving, is diluted with water to scale, shakes up, and precision measures 10ml, puts in 100ml measuring bottles, is diluted with water to quarter
Degree, shakes up, and used as need testing solution, the mensuration absorbance at the wavelength of 367nm separately takes furazolidone reference substance in right amount, accurate
It is weighed, it is measured in the same method, calculate, obtain final product；
S7：Need testing solution compares, if being deeper than 1.0%, animal groups as shown the impurity spot with reference substance solution principal spot
Contain Furaxone metabolite in knitting.
The embodiment of the present invention provides Furaxone metabolite kit in a kind of animal tissue, refers to 1, including box body 1,
The inside of box body 1 is placed with ethanol and sodium hydroxide solution, and the inside of box body 1 is installed with diaphragm successively from top to bottom
2nd, reaction film 3 and adsorptive pads 4, adsorptive pads 4 are made up of the different cotton pad in two-layer aperture, the setting of diaphragm 2, reach convenient examination
The normal reaction of agent, so as to be protected to kit, the setting of adsorptive pads 4 and reaction film 3, reached convenience is carried out to reagent
The effect of reaction.
In sum, in the animal tissue Furaxone metabolite detection method and kit, by ethanol, chlorination
The application method of sodium solution, dimethylformamide, sodium nitroprusside and sodium hydroxide test solution, has reached detection animal tissue
In whether the effect containing Furaxone metabolite, consequently facilitating the use of user, has been also convenient for testing staff to furans azoles
The detection of bupropion metabolite thing, has been also convenient for the use of the method.
Also, by the method to medicine reaction time and the control of temperature, having reached makes between medicine fully reaction
Effect, so that the speed of detection is more quick, also improves the efficiency of detection, facilitates the use of tester, is also convenient for this
The use of method.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (7)
1. in a kind of animal tissue Furaxone metabolite detection method, it is characterised in that：Comprise the following steps：
A1：Furaxone metabolite is differentiated；
A2：Furaxone metabolite is checked；
A3：Furaxone metabolite content after detection is measured.
2. in a kind of animal tissue according to claim 1 Furaxone metabolite detection method, it is characterised in that：Institute
The A1 for stating is comprised the following steps：
S1：Furaxone metabolite about 20mg is taken to be placed in box body；
S2：Plus ethanol 5ml~5.5ml and sodium hydroxide solution 3ml, that is, show red；
S3：After adding dimethylformamide 0.1ml~0.15ml dissolvings, the 5ml~6ml that adds water, sodium nitroprusside test solution 1ml
~1.6ml and sodium hydroxide test solution 1ml~1.3ml, is shaken up, and places 2 minutes, and olive-green is shown at the beginning of solution, is faded to blackish green；
S4：Furaxone metabolite 1g is taken, add water 100ml~150ml, shaken 15 minutes, filtering；
S5：Furaxone metabolite about 50mg is taken, it is accurately weighed, put in 10ml measuring bottles, dimethylformamide 5ml~8ml is added,
Tepor makes dissolving in putting water-bath, lets cool, and scale is diluted to acetone, shakes up, and as need testing solution, separately takes 5- nitryl furfurals two
Acetic acid esters reference substance is appropriate, with dimethylformamide-acetone (1:1) solution is quantitatively made the solution containing 50 μ g in every 1ml, as
Reference substance solution, draws each 20 μ l~25 μ l of above two solution, puts respectively on same silica gel g thin-layer plate, with toluene-Isosorbide-5-Nitrae
Dioxane (95:5) it is solvent, launches, dry, in 105 DEG C of dryings 5 minutes, spray (took phenylhydrazine hydrochloride with phenylhydrazine hydrochloride solution
0.75g~0.9g, plus ethanol 10ml~13ml makes dissolving, is diluted with water to 50ml~55ml, with activated carbon decolorizing, takes filtrate,
Plus hydrochloric acid 25ml, add water to 200ml), heated 5 minutes at 105 DEG C, need testing solution is molten with reference substance as shown the impurity spot
Liquid principal spot compares.
3. in a kind of animal tissue according to claim 1 Furaxone metabolite detection method, it is characterised in that：Institute
The A2 for stating includes that step is as follows：
S6：Lucifuge is operated, and takes Furaxone metabolite about 20mg, accurately weighed, is put in 250ml measuring bottles, adds dimethyl formyl
Amine 40ml~43ml, shaking makes dissolving, is diluted with water to scale, shakes up, and precision measures 10ml, dilute with water in putting 100ml measuring bottles
Release to scale, shake up, used as need testing solution, the mensuration absorbance at the wavelength of 367nm separately takes furazolidone reference substance and fits
Amount, it is accurately weighed, it is measured in the same method, calculate, obtain final product.
4. in a kind of animal tissue according to claim 1 Furaxone metabolite detection method, it is characterised in that：Institute
The A3 for stating includes that step is as follows：
S7：Need testing solution compares, if being deeper than 1.0%, animal groups as shown the impurity spot with reference substance solution principal spot
Contain Furaxone metabolite in knitting.
5. a kind of Furaxone metabolite kit in animal tissue, including box body（1）, it is characterised in that：The box body（1）'s
Inside is installed with diaphragm successively from top to bottom（2）, reaction film（3）And adsorptive pads（4）.
6. Furaxone metabolite kit in a kind of animal tissue according to claim 5, it is characterised in that：The box body
（1）Inside be placed with ethanol and sodium hydroxide solution.
7. Furaxone metabolite kit in a kind of animal tissue according to claim 5, it is characterised in that：The water suction
Pad（4）It is made up of the different cotton pad in two-layer aperture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611114150.0A CN106706617A (en) | 2016-12-07 | 2016-12-07 | Detection method and kit for detecting furazolidone metabolite in animal tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611114150.0A CN106706617A (en) | 2016-12-07 | 2016-12-07 | Detection method and kit for detecting furazolidone metabolite in animal tissues |
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| CN106706617A true CN106706617A (en) | 2017-05-24 |
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ID=58936112
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611114150.0A Pending CN106706617A (en) | 2016-12-07 | 2016-12-07 | Detection method and kit for detecting furazolidone metabolite in animal tissues |
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| CN1766624A (en) * | 2005-11-03 | 2006-05-03 | 北京望尔生物技术有限公司 | ELISA kit for detecting furazolidone metabolites and detection method thereof |
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Application publication date: 20170524 |
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