CN106683823A - Magnetic field generating device for biological study - Google Patents
Magnetic field generating device for biological study Download PDFInfo
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- CN106683823A CN106683823A CN201710141955.2A CN201710141955A CN106683823A CN 106683823 A CN106683823 A CN 106683823A CN 201710141955 A CN201710141955 A CN 201710141955A CN 106683823 A CN106683823 A CN 106683823A
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- Prior art keywords
- coil
- cooling
- magnetic
- biological study
- field generator
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F7/00—Magnets
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- Electromagnetism (AREA)
- Engineering & Computer Science (AREA)
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- Magnetic Treatment Devices (AREA)
Abstract
The invention discloses a magnetic field generating device for biological study. The device comprises a coil and a cooling device, wherein the coil is a hollow coil with a hollow interior; a cooling medium is arranged in the coil; the coil is connected with the cooling device, so that the cooling medium can flow. According to the magnetic field generating device for biological study disclosed by the invention, the interior of the coil is hollow and the cooling medium is arranged for cooling the coil, so that the interior of the device can start to cool, no cooling dead angle exists, the cooling effect is excellent and the detaching is not required.
Description
Technical field
The present invention relates to magnetic technology field, more specifically to a kind of biological study field generator for magnetic.
Background technology
With continuing to develop for science and technology, application of the people to magnetic technology is more and more extensive, such as magnetic suspension train, medical treatment
CT examination etc. is carried out by magnetic resonance.That is especially applied in terms of medical treatment is more extensive, and medical experiment is being carried out by magnetic technology
When, its requirement to temperature, environment or magnetic field is higher, the issuable different experimental result of different temperature.Existing magnetic
Typically by coil electricity magnetisation, coil own temperature after long-time is powered can change field generating means, can typically lead to
Cross coil and remove and put cold, the external measure such as air-cooled and cooled down, but the above-mentioned type of cooling is present compared with big limitation, and it needs dismounting
The cost time is more long, influence experiment effect, external air-cooled coil cooling effect to be had differences according to wind direction difference.
In sum, the poor problem of the coil cooling effect of field generator for magnetic how is efficiently solved, is current sheet
Art personnel's urgent problem.
The content of the invention
In view of this, it is an object of the invention to provide a kind of biological study field generator for magnetic, to solve biology
Learn the poor problem of the coil cooling effect of research field generator for magnetic.
In order to achieve the above object, the present invention provides following technical scheme：
A kind of biological study field generator for magnetic, including coil and cooling device, the coil are inner hollow
Hollow coil, the inside of the coil is provided with cooling medium, and the coil is connected in order to the cooling with the cooling device
Media flow.
Preferably, in above-mentioned biological study field generator for magnetic, the cooling device also includes temperature controller
And the flow control valve being connected with the temperature controller, detection temperature regulating valve of the temperature controller according to the coil
The opening degree of door.
Preferably, in above-mentioned biological study field generator for magnetic, the cooling medium is specially refrigerant or anti-
Freeze liquid.
Preferably, in above-mentioned biological study field generator for magnetic, the coil is multi-layer helical coil.
Preferably, in above-mentioned biological study field generator for magnetic, the cross section of the coil is circular or square
Shape.
Preferably, in above-mentioned biological study field generator for magnetic, the temperature adjusting scope of the temperature controller
Between 35-40 degree.
Preferably, in above-mentioned biological study field generator for magnetic, the cooling device include condenser and with institute
The kinetic pump of condenser connection is stated, the cooling device is respectively arranged at the two ends of the coil in order to circulating cooling.
The biological study field generator for magnetic that the present invention is provided, including coil and cooling device.Wherein, coil is interior
Portion is hollow and is provided with cooling medium, and being connected with cooling device for coil is flowed in order to cooling device.There is provided using the present invention
Biological study field generator for magnetic, by being set to hollow by coil inside and being provided with cooling medium coil carried out
Cooling, can be begun to cool down by therein, in the absence of cooling dead angle, good cooling results and without dismounting.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of biological study field generator for magnetic provided in an embodiment of the present invention；
Fig. 2 is the cross section structure diagram of coil provided in an embodiment of the present invention；
Fig. 3 is the schematic diagram of the neural ball that undifferentiated NSC provided in an embodiment of the present invention is formed；
Fig. 4 is the RT-PCR result figures of c-jun genes provided in an embodiment of the present invention；
Fig. 5 is the RT-PCR result figures of c-myc genes provided in an embodiment of the present invention.
Marked in accompanying drawing as follows：
Coil 1, temperature controller 2, flow control valve 3, condenser 4, cooling medium 5.
Specific embodiment
The embodiment of the invention discloses a kind of biological study field generator for magnetic, to solve biological study magnetic field
The poor problem of the coil cooling effect of generating means.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-Fig. 2 is referred to, Fig. 1 is that the structure of biological study field generator for magnetic provided in an embodiment of the present invention is shown
It is intended to；Fig. 2 is the cross section structure diagram of coil provided in an embodiment of the present invention.
In a kind of specific embodiment, the biological study that the present invention is provided includes coil 1 with field generator for magnetic
And cooling device.Wherein, coil 1 is the hollow coil 1 of inner hollow, and coil 1 is internally provided with cooling medium 5.Coil 1 with
Cooling device connection is flowed in order to cooling medium 5.Coil 1 is typically connected with cooling device by pipeline, and in junction one
As set for sealing, cooling medium 5 circulate between coil 1 and cooling device, acts on coil 1, the temperature of reduction coil 1.It is cold
But medium 5 typically can be water, conduction oil etc..As long as identical technique effect can be reached, to concrete implementation form
It is not construed as limiting.
Using the biological study field generator for magnetic that above-described embodiment is provided, coil 1 can be carried out without dismounting
Cooling, and outwards cooled down by the inside of coil 1, cool down uniform and good cooling results.
Specifically, cooling device also includes temperature controller 2 and the flow control valve 3 being connected with temperature controller 2, temperature
Opening degree of the controller 2 according to the detection temperature control valve of coil 1.Flow control valve 3 is generally located on the pipeline of input port
On, certainly in other embodiments, it is also possible to be arranged at other positions, as long as identical technique effect can be reached.
Flow control valve 3 is generally electric flow control valve 3.The temperature sensor of temperature controller 2 is generally located on the side of coil 1,
Detected by the temperature to coil 1, signal return value temperature controller 2, temperature controller 2 is anti-by the temperature of coil 1
Feedback, adjusts the opening degree of flow control valve 3, to carry out fast cooling to coil 1.Certainly, in other embodiments, it is also possible to
Temperature sensor is arranged on pipeline, is estimated with the temperature to coil 1, but there is certain mistake in said temperature detection
Difference, specifically, can need voluntarily to set the position of temperature sensor in temperature controller 2 according to actual production, in this hair
In bright protection domain.
The device can be used for the operation of science of heredity, and experimental temperature that can be accurately to cell regulates and controls, and it is to magnetic base
The expression of cause produces regulating and controlling effect, and can produce response to magnetic albumen, can also promote the propagation of cell.
Further, cooling medium 5 is specially refrigerant or anti-icing fluid.Preferably it is refrigeration to optimize cooling effect
The medium such as agent or anti-icing fluid, only more preferred embodiment herein certainly, in other embodiments, can be according to actual production
Need voluntarily to carry out the selection of cooling medium 5.
Further, coil 1 is multi-layer helical coil 1.For cooling effect more preferably, coil 1 can be set to multilayer
Spiral winding 1 so that the flowing process that cooling medium 5 can be inside coil 1 increases, the contact area of increase and coil 1 is single
Position the time in temperature drop rate faster.Multilayer herein is included more than two-layer and two-layer.Certainly, in other embodiments,
Single-layer spiral coil 1 can be selected, within the scope of the present invention.
Specifically, the cross section of coil 1 is circular or square.In order to increase the contact surface of cooling medium 5 and coil 1
, can be provided in round or square for the cross section of coil 1 by product, or may be configured as the shapes such as ellipse, rectangle, in one kind
In specific embodiment, circle is may be configured as, its flow resistance to cooling medium 5 is small.As long as identical technology can be reached
Effect, is not construed as limiting to concrete implementation form.
Further, the temperature adjusting scope of temperature controller is between 35-40 degree.Temperature adjusting in the range of this meets life
The use scope of thing research, is conducive to carrying out control accurate to the temperature of the biological operations such as test cell line, certainly, in other realities
In applying example, it is also possible to which its temperature adjusting scope is voluntarily set as needed, within the scope of the present invention.
On the basis of the various embodiments described above, cooling device includes condenser 4 and the kinetic pump being connected with condenser 4, cold
But device is respectively arranged at the two ends of coil 1 in order to circulating cooling.
Used for the ease of being circulated to cooling medium 5, improve the utilization rate of cooling medium 5, can condensed by setting
Device 4 is cooled down to cooling medium 5, and the transmission of cooling medium 5 is carried out by kinetic pump, and cooling device is separately positioned on coil 1
Two ends, typically by pipeline connect, lowered the temperature by circulating for cooling medium 5.By the circulation to cooling medium 5
Utilize, reduce use cost and be conducive to environment-friendly.
In a kind of specific embodiment, the propagation of NSC is tested by field generator for magnetic：
NSC (neural stem cell) refers to be present in nervous system, neural with being divided into
The potential of unit, astroglia and oligodendroglia such that it is able to produce a large amount of brain cell tissues, and self can be carried out more
Newly, and it is enough to provide the cell mass of a large amount of brain tissue cells.
The Wistar rats of pregnant 16 days are taken, 18, blank control group, magnetic fields group, constant temperature magnetic field group is randomly divided into, often
Group 6, extracts NSC therein respectively.Wherein, blank control group is the experimental group for not carrying out magnetic field operation, and magnetic field is made
It is experimental group that NSC is cultivated in the magnetic field device for do not contain cooling device with group；Constant temperature magnetic field group be by
The experimental group that NSC is cultivated in the magnetic field device containing cooling device.
Experimental technique and result：
The separation of NSC and culture：To after death be sterilized at the Wistar rats of pregnant 16 days, operation takes embryo's immersion
In serum-free IMDM nutrient solutions, mouse embryo brain cortex tissue is obtained, filtering is ground, using 0.125% Trypsin Induced
It is single cell suspension, scrubbed, 500r/min centrifugations, using NSC special culture media IMDM (B27 additives, 20 μ g/
L rhbFGF-2,2mmol/L Pidolidones, 20nm/L progesterone) cell is suspended again, adjustment cell concentration to 1 × 106
Individual/mL is simultaneously inoculated in blake bottle, in 37 DEG C of temperature, humidity 100%, 5%CO2Incubator, passes on 1 time for 2 days.NSC
Suspension growth, forms the cell mass for suspending, i.e., so-called neural ball in the 6th day or so.And fibroblast, vascular endothelial cell then can
Adherent growth, cell is by changing blake bottle and passage, you can removal heteroproteose cell, the NSC for being purified.
The NSC that will be passed on is respectively in incubator, field generator for magnetic (intensity 1mT), the magnetic with cooling device
Culture in field generating means (intensity 1mT).
Brdu immunocyte fluorescence chemical labelling methods：Each group cell was made single cell suspension in 1 day before detection, added dense
Spend for 10 μm of ol/L Brdu are marked.Using immunocyte fluorescence chemical technology law, Brdu antibody expressions are detected, every 24h
Detection 1 time.The computational methods of cell proliferation rate：Brdu antibody expression positive cells are proliferative cell；TCS is used
DAPI dye nucleus numbers are represented；Brdu positive cell quantities/DAPI dye nucleus numbers are the proliferation rate of NSC.Brdu
Expression positive cell and DAPI contaminate nucleus under inverted microscope, and 5 visuals field of selection are calculated, and are averaged.
Cell proliferation results such as following table：
It can thus be seen that magnetic field can accelerate the growth rate of NSC, and there is the magnetic field of cooling device
Generating means can more promote the propagation of NSC.
The expression RT-PCR detections of c-jun, c-myc：Well-grown NSC is taken, is inoculated with 1 × 108/mL
In blake bottle, packet and treatment ibid, are cultivated 2 days, collect cell.Three groups of total serum IgEs of cell are extracted respectively, carry out reverse transcription
Polymerase chain reaction, 2% agarose gel electrophoresis, then row gel imaging analysis.
The RT-PCR conditions of c-jun, c-myc gene choose such as following table：
As shown in figure 4, be the RT-PCR result figures of c-jun genes, from left to right as blank control group, magnetic fields
The RT-PCR results of group, the c-jun genes of constant temperature magnetic field group, band above is c-jun genes, and band below is GAPDH
Gene.
As shown in figure 5, be the RT-PCR result figures of c-myc genes, from left to right as blank control group, magnetic fields
The RT-PCR results of group, the c-jun genes of constant temperature magnetic field group, band above is c-myc genes, and band below is GAPDH
Gene.
It can thus be seen that after cell is subject to magnetic stimulation, the up-regulated of c-jun, c-myc gene, and with cooling
The gene expression amount of the field generator for magnetic of device is more, promotes propagation and the differentiation of NSC.
As described above, the magnetic field generator with cooling device disclosure satisfy that the requirement of constant temperature in cell cultivation process,
And the magnetic field for producing can promote the propagation of NSC.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or uses the present invention.
Various modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention
The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one
The scope most wide for causing.
Claims (7)
1. a kind of biological study field generator for magnetic, it is characterised in that including coil and cooling device, the coil is interior
The hollow hollow coil in portion, the inside of the coil is provided with cooling medium, the coil be connected with the cooling device in order to
The cooling medium flowing.
2. biological study field generator for magnetic according to claim 1, it is characterised in that the cooling device is also wrapped
Include temperature controller and the flow control valve being connected with the temperature controller, inspection of the temperature controller according to the coil
The opening degree of testing temperature control valve.
3. biological study field generator for magnetic according to claim 2, it is characterised in that the cooling medium is specific
It is refrigerant or anti-icing fluid.
4. biological study field generator for magnetic according to claim 3, it is characterised in that the coil is multilayer spiral shell
Spin line circle.
5. biological study field generator for magnetic according to claim 4, it is characterised in that the cross section of the coil
It is circular or square.
6. biological study field generator for magnetic according to claim 5, it is characterised in that the temperature controller
Temperature adjusting scope is between 35-40 degree.
7. the biological study field generator for magnetic according to claim any one of 1-6, it is characterised in that the cooling
Device includes condenser and the kinetic pump being connected with the condenser, and the cooling device is respectively arranged at the two of the coil
End is in order to circulating cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710141955.2A CN106683823A (en) | 2017-03-10 | 2017-03-10 | Magnetic field generating device for biological study |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710141955.2A CN106683823A (en) | 2017-03-10 | 2017-03-10 | Magnetic field generating device for biological study |
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| Publication Number | Publication Date |
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| CN106683823A true CN106683823A (en) | 2017-05-17 |
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ID=58828715
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| CN201710141955.2A Pending CN106683823A (en) | 2017-03-10 | 2017-03-10 | Magnetic field generating device for biological study |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2323207A (en) * | 1997-03-11 | 1998-09-16 | Elscint Ltd | Flexible hollow electrical cable |
| CN1664607B (en) * | 2004-02-16 | 2011-01-12 | 特斯拉工程有限公司 | Cooling of gradient coils for a magnetic resonance imaging device |
| CN101947359A (en) * | 2010-09-16 | 2011-01-19 | 武汉依瑞德医疗设备新技术有限公司 | Magnetic field stimulator and cooling method thereof |
| CN102110510B (en) * | 2010-12-24 | 2012-07-04 | 中国科学院深圳先进技术研究院 | Coil of magnetic resonance imaging system, and cooling device and method thereof |
-
2017
- 2017-03-10 CN CN201710141955.2A patent/CN106683823A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2323207A (en) * | 1997-03-11 | 1998-09-16 | Elscint Ltd | Flexible hollow electrical cable |
| CN1664607B (en) * | 2004-02-16 | 2011-01-12 | 特斯拉工程有限公司 | Cooling of gradient coils for a magnetic resonance imaging device |
| CN101947359A (en) * | 2010-09-16 | 2011-01-19 | 武汉依瑞德医疗设备新技术有限公司 | Magnetic field stimulator and cooling method thereof |
| CN102110510B (en) * | 2010-12-24 | 2012-07-04 | 中国科学院深圳先进技术研究院 | Coil of magnetic resonance imaging system, and cooling device and method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周建宏等: "磁生物学效应研究中磁场发生装置的选择与温度控制", 《西安文理学院学报：自然科学版》 * |
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