CN106676031A - Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides - Google Patents
Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides Download PDFInfo
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- CN106676031A CN106676031A CN201611000660.5A CN201611000660A CN106676031A CN 106676031 A CN106676031 A CN 106676031A CN 201611000660 A CN201611000660 A CN 201611000660A CN 106676031 A CN106676031 A CN 106676031A
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- Prior art keywords
- bacillus amyloliquefaciens
- seed liquor
- cgmcc
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
Abstract
The invention discloses bacillus amyloliquefaciens which is bacillus amyloliquefaciens JX-6 with a preservation number of CGMCC No. 13143 preserved in the China General Microbiological Culture Collection Center. The bacillus amyloliquefaciens JX-6 with the preservation number of CGMCC No. 13143, disclosed by the invention, can effectively degrade the carbamate pesticides and can be used for bioremediation or biological purification of soil and water bodies polluted by substances to protect ecological environment, thereby being excellent in application prospect.
Description
Technical field
The present invention relates to a bacillus amyloliquefaciens and its application in degraded carbamate chemicals for agriculture.
Background technology
Carbamate (Carbamate；General formula R O (CO) NRR) class agricultural chemicals grows up after organophosphorus ester
Synthetic pesticide, in being widely used in the agricultural productions such as deinsectization, mite killing, weeding.Carbamate chemicals for agriculture typically without special odor,
It is stable under sour environment, caustic digestion is met, solubility is higher in water.Most of kind toxicity are low compared with organophosphorus compounds, and
Deadly poisonous compound, but with carcinogenicity, can Jing respiratory tracts, alimentary canal invade body, also can percutaneously skin mucous membrane slow-absorbing.
Carbamate chemicals for agriculture has mutagenesis, teratogenesis and carcinogenesis.Sevin is for example processed in a variety of ways mouse and big
Mouse, can cause canceration, and have teratogenesis to cavy, dog, mouse, pig, chicken and duck.Acute carbamate pesticides poisoning
It is the muscarine caused because internal cholinesterase activity declines in the short time after close contact carbamate insecticides
Sample, nicotine sample and the systemic disease based on central nervous system symptom.Therefore, international cancer research institution was in handle in 2007
Carbamates is classified as 2A class carcinogenic substances.These harm how are eliminated, has been received significant attention, at present with microbial degradation
It is more effective method.
Carbamate chemicals for agriculture is conventional insecticide, before new alternative compounds are not yet found, following tens
Will be widely used in year, hence it is imperative that the degraded of research remains of pesticide.
Microbial degradation is more green and efficient one kind in various degradation of pesticide methods.However, existing bacterium is to ammonia
The degradation speed of carbamate class agricultural chemicals is slow such as, and bacterial action is in urethane compound disclosed in Publication No. CN1723276A
After thing when 10 days, degradation rate only 60%.
Be badly in need of provide it is a kind of can quickly, the new bacterial strain of efficient degradation carbamate chemicals for agriculture.
The content of the invention
The invention provides a kind of new bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and its
Application in degraded.
The invention provides a kind of bacillus amyloliquefaciens, it is common by China Committee for Culture Collection of Microorganisms
The preserving number of microorganism center preservation：The bacillus amyloliquefaciens Bacillus of CGMCC No.13143
amyloliquefaciens JX-6。
Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention, on October 24th, 2016
Preservation is China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), and preservation place is court of Beijing
The institute 3 of positive area's North Star West Road 1, preserving number is CGMCC No.13143.
Present invention also offers purposes of the bacillus amyloliquefaciens in degraded carbamate chemicals for agriculture.
Wherein, the bacillus amyloliquefaciens are the bacillus amyloliquefaciens that preserving number is CGMCC No.13143
Bacillus amyloliquefaciens JX-6。
Wherein, the carbamate chemicals for agriculture is (1- naphthyls)-N- methyl carbamate, i.e. sevin.
Present invention also offers a kind of method of degrading pesticide, comprises the steps：
(1) bacillus amyloliquefaciens are taken, recovery prepares seed liquor；
(2) seed liquor is added in pending sample, is mixed, you can.
In step (1), the bacillus amyloliquefaciens are the bacillus amyloliquefaciens of preserving number CGMCC No.13143
Bacillus amyloliquefaciens JX-6。
In step (1), the concentration of the seed liquor is not less than 106cfu/mL。
Preferably, the concentration of the seed liquor is 106~109cfu/mL。
The seed liquor is prepared as follows：Bacillus amyloliquefaciens are taken, LB medium cultures are used, is adjusted dense
Degree, you can.
Bacillus amyloliquefaciens JX-6 (Bacillus amyloliquefaciens JX-6) of the present invention, its can efficiently,
Fast degradation carbamate chemicals for agriculture, for the soil of these Substances Pollutions, the biological prosthetic or biological cleaning of water body, protection
Ecological environment, with fabulous application prospect.
Obviously, the above of the invention, according to the ordinary technical knowledge and customary means of this area, without departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification of other various ways can also be made, is replaced or is changed.
By the following examples the specific embodiment of form, remakes further specifically to the above of the present invention
It is bright.But this scope for being interpreted as above-mentioned theme of the invention should not be only limitted to Examples below.It is all based on the above of the present invention
The technology realized belongs to the scope of the present invention.
Description of the drawings
Fig. 1 is the bacterium colony cultural characteristic that degradation bacteria strains JX-6 cultivates 48h.
Fig. 2 is the cellular morphology figure of degradation bacteria strains JX-6.
Fig. 3 is the degraded situation of degradation bacteria strains JX-6 in experimental example 1.
Fig. 4 is the chromatogram of degradation bacteria strains JX-6 degraded sevins and its product in experimental example 1.
Specific embodiment
Culture medium of the present invention：
Luria-Bertani culture mediums (LB)：Yeast extract (Yeast extract) 5.0g；Tryptone (Casein
tryptone)10g；Sodium chloride (Sodium chloride) 10g；Distilled water (H2O)1000mL；Adjust pH to 7.0；This solid training
Foster base is added again made by 20g/L agar (Agar) in liquid medium within.Culture is based on 121 DEG C, and 20min is standby for sterilizing.
The separation identification of the bacillus amyloliquefaciens of the present invention of embodiment 1
1st, separate
Take vinegar and pay for 4 parts of sample, mix thoroughly respectively, respectively take 5g, in being placed in the 250mL conical flasks equipped with 30mL LB culture mediums, in
10min is heated at 80 DEG C, be cooled to room temperature add sevin to make its mass concentration is 50 mg/L, is vibrated in 30 DEG C, 180r/min
Culture 48h.Culture carries out successively again 3 enrichment cultures after terminating by 5% inoculum concentration, and 48h is cultivated every time, and steps up culture
Sevin concentration in base, is allowed to respectively reach 100mg/L, 200mg/L, 400mg/L, screens the one plant of pesticide degradation bacteria for obtaining,
Numbering is JX-6.
2nd, identify
(1) form, physiological and biochemical property
Form：Picking bacterial strain JX-6 to LB solid medium flat board cultures, record its colony morphology characteristic, picking single bacterium colony
Smear, Gram's staining are carried out, observed and recorded color, size, shape, arrangement etc. under 10 × 100 times of oil mirrors.
Physiology and biochemistry identification referring to《The outstanding Bacteria Identification handbook of uncle》8th edition.
The Morphological Identification (as shown in Figure 1 and Figure 2) of bacterial strain JX-6：Gram-positive, bacillus, bacterium colony is trained in LB
It is in pale opaque colony on foster base, non-reflective, surface ruffle has protuberance, and edge is irregular.
The bacterial strain JX-6 Physiology and biochemistry qualification results of table 1
(2) 16S rDNA identifications
The PCR of bacterial strain 16S rDNA genes is expanded, PCR conditions：94 DEG C of denaturations 5min；Then 94 DEG C, 45s, 56 DEG C,
45s, 72 DEG C, 90s, circulate 30 times；Last 72 DEG C of extensions 10min；PCR primer Jing electrophoresis detection, amplified production holds up section by Chengdu
Zi Xi Bioisystech Co., Ltd is sequenced, and the 16S rDNA sequences of bacterial strain JX-6 are as shown in SEQ ID NO.1:
CTGGCTCTAAAGGTTACCTCACCGACTTCGGGTGTTAAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGG
GAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGAT
CCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCG
GTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCAT
CCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCAACTAAGATCAAGGGTT
GCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCC
CGAAGGGGACGTCCTATCTCTAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAA
ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGG
AGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTGGAC
TACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAGAGTCGCCTTCG
CCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTCAAGTT
CCCCAGTTTCCAATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAACCGCCTGCGAGCCCTTT
ACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCT
GGTTAGGTACCGTCAAGGTGCCGCCCTATTTGAACGGCACTTGTTCTTCCCTAACAACAGAGCTTTACGATCCGAAA
ACCTTCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAG
GAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCC
GTTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTCTGAACCA
TGCGGTTCAAACAACCATCCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAGTCTTACAGGCAGGTTACCCACGTG
TTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCCCATC。
Determine, sequence carries out Blast homology alignments in sequencing result and GenBank databases, the strain sequence with
Bacillus amyloliquefaciens strain gene sequence very high homologies.
To sum up, combining form feature, physiological and biochemical property, 16S rDNA qualification results, by separation strains JX-6 of the present invention mirror
It is set to bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and is named as bacillus amyloliquefaciens
Bacillus amyloliquefaciens JX-6, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
Center, preserving number is CGMCC No.13143.
The sevin that embodiment 2 is degraded in soil using bacillus amyloliquefaciens of the present invention
Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention are taken, preserving number is CGMCC
No.13143, abbreviation bacterial strain JX-6.Actication of culture：In picking bacterial strain JX-6 to LB fluid nutrient mediums, 30 DEG C, 180r/min vibrations
Culture 24h；Then continuously line, the culture of picking single bacterium colony twice (30 DEG C), picking single bacterium colony is seeded to the culture of 30 DEG C of LB inclined-planes
2d is standby.Thalline is washed on lower inclined plane with SPSS and adjust cell concentration for 106Cfu/mL, makes seed liquor.
Seed liquor is added in pending soil, is mixed, you can.
Embodiment 3 is using the sevin in bacillus amyloliquefaciens degradation water of the present invention
Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention are taken, preserving number is CGMCC
No.13143, abbreviation bacterial strain JX-6.Actication of culture：In picking bacterial strain JX-6 to LB fluid nutrient mediums, 30 DEG C, 180r/min vibrations
Culture 24h；Then continuously line, the culture of picking single bacterium colony twice (30 DEG C), picking single bacterium colony is seeded to the culture of 30 DEG C of LB inclined-planes
2d is standby.Thalline is washed on lower inclined plane with SPSS and adjust cell concentration for 108Cfu/mL, makes seed liquor.
Seed liquor is added in pending sample water, is mixed, you can.
The sevin that embodiment 4 is degraded in soil using bacillus amyloliquefaciens of the present invention
Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention are taken, preserving number is CGMCC
No.13143, abbreviation bacterial strain JX-6.Actication of culture：In picking bacterial strain JX-6 to LB fluid nutrient mediums, 30 DEG C, 180r/min vibrations
Culture 24h；Then continuously line, the culture of picking single bacterium colony twice (30 DEG C), picking single bacterium colony is seeded to the culture of 30 DEG C of LB inclined-planes
2d is standby.Thalline is washed on lower inclined plane with SPSS and adjust cell concentration for 109Cfu/mL, makes seed liquor.
Seed liquor is added in pending soil, is mixed, you can.
Prove beneficial effects of the present invention with the mode of experimental example below：
The method that experimental example 1 adopts bacillus amyloliquefaciens of the present invention degraded sevin
1 material and instrument
1.1 materials
Bacterial classification：Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention, preserving number is
CGMCC No.13143, abbreviation bacterial strain JX-6.
Sevin：Purchased from Chinese Industrial Standards (CIS) material center.
1.2 key instruments
WondaSil C18 posts, purchased near (Shanghai) the commerce and trade Co., Ltd of Shimadzu skill.
2 test methods
Actication of culture：In picking bacterial strain JX-6 to LB fluid nutrient mediums, 30 DEG C, 180r/min shaken cultivations 24h；Then connect
Twice (30 DEG C), it is standby that picking single bacterium colony is seeded to 30 DEG C of LB inclined-planes culture 2d for continuous line, the culture of picking single bacterium colony
Thalline is washed on lower inclined plane with SPSS and adjust cell concentration for 106Cfu/mL, makes seed liquor.
The seed liquor of degradation bacteria strains JX-6 is inoculated in by 5% inoculum concentration western containing 100mg/L, 200mg/L, 400mg/L
In the LB fluid nutrient mediums of denapon, 30mL/250mL conical flasks are dispensed, if 5% sterilized water of inoculation is blank, 30 DEG C,
180r/min shaken cultivations, sample time is 1 bottle of the sampling per 12h during 0h-96h, then uniform nutrient solution is taken from conical flask
1mL, with methanol constant volume to 10mL, mixes and 10min is centrifuged after 10000r/min, takes supernatant and analyzes for liquid chromatogram (HPLC)
With.
Condition determination：Chromatographic column is WondaSil C18 posts (150mm × 4.60mm, 5.0 μm)；Mobile phase is acetonitrile:Water
(85:15, V/V), flow velocity 0.5mL/min；UV-detector, its wavelength is 220nm；The μ L of sample size 10.
3 results and analysis
As a result as shown in figure 3, degradation bacteria strains JX-6 is tieed up in 72h to 50mg/L, 100mg/L, 200mg/L, 400mg/L west
The degradation rate of cause is successively 82%, 75%, 75%, 62%.
Experimental result illustrates that bacillus amyloliquefaciens JX-6 of the present invention can effectively degrade sevin, and degradation speed is fast, drop
Solution rate is high；Such as Fig. 4, degradation bacteria strains JX-6 can degrade the intermediates of sevin, and 8.57min peaks are target in chromatogram
Peak (sevin), As time goes on, sevin target peak (0-72h) while diminishing occurs one newly in 9.12min
Peak and gradually rise (0-72h), and in 72-120h reduce, judge 9.12min peaks for sevin degraded centre product
Thing.
The sevin that experimental example 2 is degraded in sewage using bacillus amyloliquefaciens of the present invention
1 material and instrument
1.1 materials
Bacterial classification：Bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of the present invention, preserving number is
CGMCC No.13143, abbreviation bacterial strain JX-6.
Sevin：Purchased from Chinese Industrial Standards (CIS) material center.
1.2 key instruments
WondaSil C18 posts, purchased near (Shanghai) the commerce and trade Co., Ltd of Shimadzu skill.
2 test methods
Actication of culture：In picking bacterial strain JX-6 to LB fluid nutrient mediums, 30 DEG C, 180r/min shaken cultivations 24h；Then connect
Twice (30 DEG C), it is standby that picking single bacterium colony is seeded to 30 DEG C of LB inclined-planes culture 2d for continuous line, the culture of picking single bacterium colony.
Thalline is washed on lower inclined plane with SPSS and adjust cell concentration for 106Cfu/mL, makes seed liquor.
A certain amount of pedotheque is taken from the vegetable soil of Sichuan Province Chengdu, addition sevin makes its mass concentration about
For 100mg/L, the seed liquor of degradation bacteria strains is inoculated with into (experimental group) by 5% inoculum concentration, if 5% sterilized water of inoculation is blank right
According to 30 DEG C of cultures, the sampling per 24h states the residual quantity that method determines DBP in pedotheque with experimental example 1.
By the residual quantity of sevin in HPLC detection samples, sevin changes of contents is less in blank sewage sample, real
The sewage sample for testing group is nearly no detectable sevin (table 2) in 4d.
Applications of the bacterial strain JX-6 of table 2 in sewage
Experimental result illustrates that bacillus amyloliquefaciens JX-6 of the present invention can effectively degrade sevin, and degradation speed is fast, drop
Solution rate is high.
To sum up, the isolated one plant of new bacillus amyloliquefaciens JX-6 (Bacillus of the present invention
Amyloliquefaciens JX-6), its can efficiently, the carbamate chemicals for agriculture such as fast degradation sevin, environment is repaiied
Multiple effect is good, with fabulous application prospect.
SEQUENCE LISTING
<110>Chengdu Medical College
<120>A kind of bacillus amyloliquefaciens and its application in degraded carbamate chemicals for agriculture
<130> GY044-16P1500
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1394
<212> DNA
<213> 16S rDNA
<400> 1
ctggctctaa aggttacctc accgacttcg ggtgttaaaa ctctcgtggt gtgacgggcg 60
gtgtgtacaa ggcccgggaa cgtattcacc gcggcatgct gatccgcgat tactagcgat 120
tccagcttca cgcagtcgag ttgcagactg cgatccgaac tgagaacaga tttgtgggat 180
tggcttaacc tcgcggtttc gctgcccttt gttctgtcca ttgtagcacg tgtgtagccc 240
aggtcataag gggcatgatg atttgacgtc atccccacct tcctccggtt tgtcaccggc 300
agtcacctta gagtgcccaa ctgaatgctg gcaactaaga tcaagggttg cgctcgttgc 360
gggacttaac ccaacatctc acgacacgag ctgacgacaa ccatgcacca cctgtcactc 420
tgcccccgaa ggggacgtcc tatctctagg attgtcagag gatgtcaaga cctggtaagg 480
ttcttcgcgt tgcttcgaat taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat 540
tcctttgagt ttcagtcttg cgaccgtact ccccaggcgg agtgcttaat gcgttagctg 600
cagcactaag gggcggaaac cccctaacac ttagcactca tcgtttacgg cgtggactac 660
cagggtatct aatcctgttc gctccccacg ctttcgctcc tcagcgtcag ttacagacca 720
gagagtcgcc ttcgccactg gtgttcctcc acatctctac gcatttcacc gctacacgtg 780
gaattccact ctcctcttct gcactcaagt tccccagttt ccaatgaccc tccccggttg 840
agccgggggc tttcacatca gacttaagaa accgcctgcg agccctttac gcccaataat 900
tccggacaac gcttgccacc tacgtattac cgcggctgct ggcacgtagt tagccgtggc 960
tttctggtta ggtaccgtca aggtgccgcc ctatttgaac ggcacttgtt cttccctaac 1020
aacagagctt tacgatccga aaaccttcat cactcacgcg gcgttgctcc gtcagacttt 1080
cgtccattgc ggaagattcc ctactgctgc ctcccgtagg agtctgggcc gtgtctcagt 1140
cccagtgtgg ccgatcaccc tctcaggtcg gctacgcatc gtcgccttgg tgagccgtta 1200
cctcaccaac tagctaatgc gccgcgggtc catctgtaag tggtagccga agccaccttt 1260
tatgtctgaa ccatgcggtt caaacaacca tccggtatta gccccggttt cccggagtta 1320
tcccagtctt acaggcaggt tacccacgtg ttactcaccc gtccgccgct aacatcaggg 1380
agcaagctcc catc 1394
Claims (9)
1. a kind of bacillus amyloliquefaciens, it is characterised in that：It is commonly micro- by China Committee for Culture Collection of Microorganisms
The preserving number of Bio-Centers preservation：The bacillus amyloliquefaciens Bacillus amyloliquefaciens of CGMCC No.13143
JX-6。
2. bacillus amyloliquefaciens degraded carbamate chemicals for agriculture in purposes.
3. purposes according to claim 2, it is characterised in that：It is CGMCC that the bacillus amyloliquefaciens are preserving numbers
The bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of No.13143.
4. purposes according to claim 2, it is characterised in that：The carbamate chemicals for agriculture is (1- naphthyls)-N- first
Aminocarbamic acid ester.
5. a kind of method of degrading pesticide, it is characterised in that：Comprise the steps：
(1) bacillus amyloliquefaciens are taken, recovery prepares seed liquor；
(2) seed liquor is added in pending sample, is mixed, you can.
6. method according to claim 5, it is characterised in that：In step (1), the bacillus amyloliquefaciens are preserving numbers
The bacillus amyloliquefaciens Bacillus amyloliquefaciens JX-6 of CGMCC No.13143.
7. method according to claim 6, it is characterised in that：In step (1), the concentration of the seed liquor is not less than
106cfu/mL。
8. method according to claim 7, it is characterised in that：The concentration of the seed liquor is 106~109cfu/mL。
9. the method according to claim 7 or 8, it is characterised in that：The seed liquor is prepared as follows：Take solution
Bacillus amyloliquefacienses, use LB medium cultures, adjust concentration, you can.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101994072B1 (en) * | 2019-03-13 | 2019-06-27 | 양국산 | Biological microbial treating agent for removing agrochemical |
| CN111235053A (en) * | 2019-12-25 | 2020-06-05 | 宁波市农业科学研究院 | Bacillus amyloliquefaciens Z1112 and application thereof |
| KR102553903B1 (en) * | 2022-10-24 | 2023-07-11 | 재단법인 농축산용미생물산업육성지원센터 | Composition for decomposing pesticides |
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| US5958758A (en) * | 1997-08-04 | 1999-09-28 | Biosun Systems Corporation | Treatment of animal waste |
| CN102776146A (en) * | 2012-08-19 | 2012-11-14 | 南京农业大学 | Bacillus amyloliquefaciens and application thereof |
| CN103013948A (en) * | 2012-12-27 | 2013-04-03 | 江南大学 | Method for producing ethyl carbamate enzyme by purifying lysinibacillus fusiformis |
| CN105420140A (en) * | 2015-09-17 | 2016-03-23 | 河北农业大学 | Bacillus amyloliquefaciens and application thereof |
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| KR101994072B1 (en) * | 2019-03-13 | 2019-06-27 | 양국산 | Biological microbial treating agent for removing agrochemical |
| WO2020184771A1 (en) * | 2019-03-13 | 2020-09-17 | 양국산 | Microbial biological treatment agent for removing agricultural chemicals |
| CN111235053A (en) * | 2019-12-25 | 2020-06-05 | 宁波市农业科学研究院 | Bacillus amyloliquefaciens Z1112 and application thereof |
| CN111235053B (en) * | 2019-12-25 | 2021-08-03 | 宁波市农业科学研究院 | Bacillus amyloliquefaciens Z1112 and application thereof |
| KR102553903B1 (en) * | 2022-10-24 | 2023-07-11 | 재단법인 농축산용미생물산업육성지원센터 | Composition for decomposing pesticides |
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