CN106667994A - Application of oligomeric polyphenol compounds to preparation of gram-positive drug-resistant bacterium resistant products - Google Patents
Application of oligomeric polyphenol compounds to preparation of gram-positive drug-resistant bacterium resistant products Download PDFInfo
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- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
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Abstract
The invention discloses application of oligomeric polyphenol compounds to preparation of gram-positive drug-resistant bacterium resistant products. The oligomeric polyphenol compounds are dimer and trimer oligomeric polyphenol compounds of a trans form, the dimer and trimer resveratrol oligomeric polyphenol compounds are a compound 1: trans-D-viniferin and a compound 2: gentian H, the drug-resistant bacterium resistance of the dimer is higher than that of the trimer, an MIC (minimal inhibitory concentration) value of the drug-resistant bacterium resistance of the trimer trans-D-viniferin is 8 mu g/mL, and an MIC value of the drug-resistant bacterium resistance of the trimer gentian H is 16 mu g/mL. The drug-resistant bacterium resistant spectrum of the two compounds is disclosed for the first time to be a gram-positive drug-resistant bacterium, and an evaluation result of the MIC values of the drug-resistant bacterium resistance shows that the compounds are natural compounds with antibiotic effects, and has great scientific significant and high application value for researches on the natural oligomeric polyphenol compounds.
Description
Technical field
The invention belongs to natural product application, and in particular to oligomeric polyphenol compound is preparing resisting gram-positive
The application of fastbacteria product.
Background technology
Dimer and the oligomeric polyphenol compound of trimer resveratrol, be with stilbene skeleton unit and its
The general name of polymer.Lot of documents reports that such oligomeric polyphenol structure has strong antibacterial action.With isolation technics and spectrum
The progress of analytical technology, fitochemical studies report, increasing such polymer compounds are found.From nineteen ninety-five so far
Till, document report is respectively from the Radix Stemonae, Fructus Cannabiss, papilionaceous flower, pulse family Chrysolophus, Compositae, Fructus Momordicae charantiae section, orchid family, Gnetaceae, Fructus Vitis viniferae
Isolate in the 33 not equal platymisciums such as section, Moraceae, Paeoniaceae and obtain such oligomeric polyphenol compound more than 500.
The activity of such compound is mainly manifested in：Antiinflammatory, antioxidation, antitumor, anti-AIDS and hepatoprotective effect；To epoxidase, front
Row parathyrine H2 synzyme, Protein kinase C etc. have strong inhibitory activity and antifungal and antibacterial activity etc..In recent years we grind
Study carefully discovery, because such compound has the multiformity of structure diversity and biological activity, the molecule of this kind of oligomer in part is also
Strong antimicrobial agent activity is shown, thus the antibiotic sample having is acted on.The nature of lot of documents research report is oligomeric
Polyphenol compound has extensive antibacterial activity.We are it has been found that the antimicrobial agent that has of trans-D- grape elements and Radix Gentianae H
Activity, and declared patent " natural antibiotics feed additive containing Radix Gentianae H and its preparation method and application "（Shen
Please number：2015108551149）.
The content of the invention
The goal of the invention of the present invention is to disclose oligomeric polyphenol compound to prepare resisting gram-positive fastbacteria product
Using, and described oligomeric polyphenol compound is the dimer and the oligomeric polyphenol compound of trimer of transconfiguration.
Further, described dimer and the oligomeric polyphenol compound of trimer resveratrol are compound 1：Instead
Formula-D- grape elements and compound 2：Radix Gentianae H, structural formula is respectively：
Compound 1
Compound 2
Further, described dimer antimicrobial agent is higher compared with the antimicrobial agent of trimer, and described dimer is anti-
The antimicrobial agent MIC value of formula-D- grape elements is 8 μ g/mL, and the antimicrobial agent MIC value of trimer Radix Gentianae H is 16 μ g/mL.
Compared with prior art, the present invention at least tool has the advantage that and beneficial effect：
The present invention first public two compound antimicrobial agents spectrum is Gram-positive fastbacteria, and antimicrobial agent is minimum antibacterial dense
The evaluation result of degree MIC value, is the native compound for having antibiotic effect, and the result is to such natural oligomeric polyphenolic substance
Research has important scientific meaning and using value.Such compound is contained with the seed food of Vitaceae, Moraceae and Paeoniaceae these plants
Amount highest, they are widely present in the fruit of medicine food dual purpose plant and seed food, can be used as functional health care product additive and important
Antibiotic sample medicinal raw material, i.e., oligomeric polyphenol compound will prepare resisting gram-positive fastbacteria medicine, additive, health care
Have a wide range of applications on the products such as product, food.
Specific embodiment
First, with regard to the antimicrobial agent evaluation of two compounds
Minimal inhibitory concentration MICs (Minimum Inhibitory Concentration) is continuous using external constant meat soup
Dilution.Concrete grammar is as follows.
1. prepared by antibacterials stock solution
Positive control antibiotic vancomycin and all bacterial strains, directly grind purchased from Beijing Chinese Academy of Medical Sciences medical biotechnology
Study carefully institute.
Concrete configuration refers to NCCLS sensitivity testing to antibacterials operation standards, tested antibacterials Formulas I and Formula II two
Oligomeric phenolic configuration concentration is not lower than 1000 μ g/ml.Such as need to prepare the antibiotic storage that 100 ml concentration are 1280 μ g/ml
Liquid, sets the effective force of its medicine as 750 μ g/mg.The amount for accurately weighing antibiotic powder with analytical balance is 182.6mg.Root
Calculating required diluent consumption according to formula is：（182.6mg×750μg/ml）/ 1280 μ g/ml=107.0ml, then by 182.6
Mg antibiotic powders are dissolved in 107.0ml diluent.The antibacterials stock solution for preparing should be stored in less than -60 DEG C rings
Border, storage life was less than 4-6 month.
2. prepared by drug sensitive test culture medium
According to NCCLS sensitivity testing to antibacterials operation standards, culture medium uses Mueller-Hinton（MH）Meat soup,
pH7.2~7.4.In addition 2% is added in meat soup（W/V）Sodium Chloride.The MH meat soups of requirement are prepared by the requirement of manufacturing firm.
3. the preparation of inoculum adopts direct bacterium colony suspension preparation method.
Methicillin-resistant Staphylococcus directly take 18 ~ 24h of culture.Take 1-2 methicillin-resistant Staphylococcus bacterium colony to put
In above-mentioned 2 culture fluid, bacteria suspension of 0.5 Maxwell than turbid standard is deployed into.
Take MH meat soups by above-mentioned bacteria suspension carry out 1: 1 dilution after it is standby.Note to be inoculated with 15 minutes and prepare
Inoculum, and a inoculum Secondary Culture on non-selective agar flat board is taken, to check inoculum purity.
4. the preparation and bacterium solution inoculation of antibacterials are diluted
Take sterile test tube（13x100mm）13, it is arranged in a row, in addition to the 1st pipe adds 1.6mlMH meat soups, remaining often pipe addition MH
Meat soup 1ml, in the 1st pipe Formulas I antibacterials stock solution is added（Such as 1280 μ g/ml）0.4ml is mixed, and is then drawn 1ml to the 2nd and is managed,
Draw the pipes of 1ml to the 3rd after mixing again, so continuous doubling dilution is to the 11st pipe, and absorption 1ml is discarded from the 11st pipe, the 12nd
Manage as the growth control of not drug containing.Now each pipe drug level be followed successively by 256,128,64,32,16,8,4,2,1,0.5,
0.25μg/ml.Then each 1ml of the above-mentioned inoculum for preparing is added in every pipe, make the final bacterial concentration of every pipe be about 5 ×
105CFU/ml.1st pipe is respectively 128,64,32,16,8,4,2,1,0.5,0.25,0.125 μ g/ to the 11st pipe drug level
Ml. equally, Formula II oligomeric phenolic compound and positive control vancomycin are as above equally operated.By the dilution pipe close being inoculated with
Good stopper incubation, puts and be incubated in 35 DEG C of normal air incubators full 24h.
5th, result judges and explains
Before the MIC of reading and report institute test strain, should check whether the bacterial growth situation of growth control pipe is good, while
The Secondary Culture situation of inoculum should also be checked to determine if pollution, whether the MIC value of Quality-control strains is in Quality Control scope.
With perusal, medicine least concentration pipe is without bacterial growth person, the MIC of as tested bacterium.Compare suppression with Growth positive control tube
80% bacterial growth pipe drug level is made for tested bacterium MIC.
The natural oligomeric polyphenolic substance I of table 1. and II antimicrobial agents are determined(MIC Platings, unit μ g/ml)
As above, natural oligomeric polyphenolic substance I and II antimicrobial agent measurement results are shown in Table shown in 1..
As can be known from Table 1,1）Formula I（Trans- ε-Viniferin, trans-D- grape elements）Anti- methicillin-resistant
Staphylococcuses（MRSA）With resistance to staphylococcus epidermidiss（MSSE）It is 8 μ g/ml；Formula II（Gentin H, Radix Gentianae H）Resist resistance to epidermis
Staphylococcuses（MSSE）For 16 μ g/ml, overriding resistance enterococcus faecalis（VRE）MIC value be 16 μ g/ml.They show strong resisting
Gram-positive fastbacteria activity, 128 μ g/ml, therefore nonreactive gram-negative are all higher than to all of Gram-negative fastbacteria
The activity of property fastbacteria.2）From Formula I to Formula II, the change actually from dimer to trimer, water solublity is gradually
Reduce, antimicrobial agent reduced activity.Therefore compound I dimer antimicrobial agents are higher.3）By to two compound structure effects
Relation comparative study understands that compound I and II are anti-configuration；Contrary cis-configuration either I or formula II, experiment
Show without antimicrobial agent activity.
2nd, natural oligomeric polyphenolic substance I and II antimicrobial agents determine operation embodiment：
Embodiment 1. extracts compound I and II from Semen Vitis viniferae and antimicrobial agent is determined
（1）The extraction of compound I and II：
7 kilograms of Semen Vitis viniferae, is soaked with 70% alcohol at normal temperature, continues 3 days, is repeated twice.By the leachate vacuum distillation for obtaining, obtain
To the g of ethanol extract 400.300 g ethanol extracts are taken, after ethanol extract is dissolved with solvent, after taking macroporous resin decolouring, then is used
800 g silica gel（100-200 mesh）Mix sample to load.First eluting is carried out with the ethyl acetate of 5 times of column volumes, then with 5 times of cylinders
Long-pending acetone carries out eluting.Eluent does respectively vacuum distillation, obtains the g of ethyl acetate extract 80 and the g extractum of acetone 87.
Acetone extract is taken, by Waters high performance liquid preparative chromatographies（Methanol：Water 55:45, flow velocity 8ml/min, YMC
ODS C18,250*20mm）, component F1 is obtained, F2, F3 and F4. component F1 passes through Waters high performance liquid chromatography（Methanol：Water
50:50, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound II（Gnetin H）；Component F3 passes through Waters
High performance liquid chromatography（Methanol：Water 45:55, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound I（Trans-
Trans-D- the grape elements of ε-Viniferin）.
（2）The antimicrobial agent of compound I and II is determined：
1. prepared by antibacterials stock solution
Positive control antibiotic vancomycin and all bacterial strains, directly grind purchased from Beijing Chinese Academy of Medical Sciences medical biotechnology
Study carefully institute.
Concrete configuration refers to NCCLS sensitivity testing to antibacterials operation standards, tested antibacterials Formulas I and Formula II two
Oligomeric phenolic configuration concentration is not lower than 1000 μ g/ml.The amount for accurately weighing antibiotic powder with analytical balance is
171.2mg.According to formula calculate needed for diluent consumption be：（171.2mg×750μg/ml）/ 1200 μ g/ml=107.0ml, so
171.2mg antibiotic powders are dissolved in 107.0ml diluent afterwards.The antibacterials stock solution for preparing should be stored in -60
Environment below DEG C, storage life was less than 4-6 month.
2. prepared by drug sensitive test culture medium
According to NCCLS sensitivity testing to antibacterials operation standards, culture medium uses Mueller-Hinton（MH）Meat soup,
pH7.2~7.4.In addition 2% is added in meat soup（W/V）Sodium Chloride.The MH meat soups of requirement are prepared by the requirement of manufacturing firm.
3. the preparation of inoculum adopts direct bacterium colony suspension preparation method.Methicillin-resistant Staphylococcus directly take culture 18 ~
24h.Take 1-2 methicillin-resistant Staphylococcus bacterium colony to put in above-mentioned 2 culture fluid, be deployed into 0.5 Maxwell than turbid standard
Bacteria suspension.
Take MH meat soups by above-mentioned bacteria suspension carry out 1: 1 dilution after it is standby.Note to be inoculated with 15 minutes and prepare
Inoculum, and a inoculum Secondary Culture on non-selective agar flat board is taken, to check inoculum purity.
4. the preparation and bacterium solution inoculation of antibacterials are diluted
Take sterile test tube（13x100mm）13, it is arranged in a row, in addition to the 1st pipe adds 1.6mlMH meat soups, remaining often pipe addition MH
Meat soup 1ml, in the 1st pipe Formulas I antibacterials stock solution is added（Such as 1200 μ g/ml）0.4ml is mixed, and is then drawn 1ml to the 2nd and is managed,
Draw the pipes of 1ml to the 3rd after mixing again, so continuous doubling dilution is to the 11st pipe, and absorption 1ml is discarded from the 11st pipe, the 12nd
Manage as the growth control of not drug containing.Now each pipe drug level be followed successively by 256,128,64,32,16,8,4,2,1,0.5,
0.25μg/ml.Then each 1ml of the above-mentioned inoculum for preparing is added in every pipe, make the final bacterial concentration of every pipe be about 5 ×
105CFU/ml.1st pipe is respectively 128,64,32,16,8,4,2,1,0.5,0.25,0.125 μ g/ to the 11st pipe drug level
Ml. equally, Formula II oligomeric phenolic compound and positive control vancomycin are as above equally operated.By the dilution pipe close being inoculated with
Good stopper incubation, puts and be incubated in 35 DEG C of normal air incubators full 24h.
5th, result judges and explains
Before the MIC of reading and report institute test strain, should check whether the bacterial growth situation of growth control pipe is good, while
The Secondary Culture situation of inoculum should also be checked to determine if pollution, whether the MIC value of Quality-control strains is in Quality Control scope.
With perusal, medicine least concentration pipe is without bacterial growth person, the MIC of as tested bacterium.Compare suppression with Growth positive control tube
80% bacterial growth pipe drug level is made for tested bacterium MIC value.
The present embodiment two compound antimicrobial agent MIC value test results of gained are as shown in table 1.
Embodiment 2. extracts compound I and II from Paeonia ostii white peony seed and antimicrobial agent is determined
（1）The extraction of compound I and II：
7 kilograms of Paeonia ostii white peony seed is purchased, shelling is dried.Seed of Flos Moutan shell is crushed and is soaked with 70% alcohol at normal temperature, continue 3 days, weight
Again twice.By the leachate vacuum distillation for obtaining, the g of ethanol extract 400 is obtained.300 g ethanol extracts are taken, ethanol extract is used
After solvent dissolving, take after macroporous resin decolourizes, then with 800 g silica gel（100-200 mesh）Mix sample to load.First with 5 times of column volumes
Ethyl acetate carry out eluting, then carry out eluting with the acetone of 5 times of column volumes.Eluent does respectively vacuum distillation, obtains second
The g of acetoacetic ester extractum 110 and the g extractum of acetone 150.
Acetone extract is taken, by Waters high performance liquid preparative chromatographies（Methanol：Water 55:45, flow velocity 8ml/min, YMC
ODS C18,250*20mm）, component F1 is obtained, F2, F3 and F4. component F1 passes through Waters high performance liquid chromatography（Methanol：Water
50:50, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound II（Gnetin H）；Component F3 passes through Waters
High performance liquid chromatography（Methanol：Water 45:55, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound I（Trans-
Trans-D- the grape elements of ε-Viniferin）.
（2）The antimicrobial agent of compound I and II is determined：
1. prepared by antibacterials stock solution
Positive control antibiotic vancomycin and all bacterial strains, directly grind purchased from Beijing Chinese Academy of Medical Sciences medical biotechnology
Study carefully institute.
Concrete configuration refers to NCCLS sensitivity testing to antibacterials operation standards, tested antibacterials Formulas I and Formula II two
Oligomeric phenolic configuration concentration is not lower than 1000 μ g/ml.Such as need to prepare the antibiotic storage that 100 ml concentration are 1150 μ g/ml
Liquid, sets the effective force of its medicine as 750 μ g/mg.The amount for accurately weighing antibiotic powder with analytical balance is 164.1mg.Root
Calculating required diluent consumption according to formula is：（164.1mg×750μg/ml）/ 1150 μ g/ml=107.0ml, then by 164.1
Mg antibiotic powders are dissolved in 107.0ml diluent.The antibacterials stock solution for preparing should be stored in less than -60 DEG C rings
Border, storage life was less than 4-6 month.
2. prepared by drug sensitive test culture medium
According to NCCLS sensitivity testing to antibacterials operation standards, culture medium uses Mueller-Hinton（MH）Meat soup,
pH7.2~7.4.In addition 2% is added in meat soup（W/V）Sodium Chloride.The MH meat soups of requirement are prepared by the requirement of manufacturing firm.
3. the preparation of inoculum adopts direct bacterium colony suspension preparation method.Methicillin-resistant Staphylococcus directly take culture 18 ~
24h.Take 1-2 methicillin-resistant Staphylococcus bacterium colony to put in above-mentioned 2 culture fluid, be deployed into 0.5 Maxwell than turbid standard
Bacteria suspension.
Take MH meat soups by above-mentioned bacteria suspension carry out 1: 1 dilution after it is standby.Note to be inoculated with 15 minutes and prepare
Inoculum, and a inoculum Secondary Culture on non-selective agar flat board is taken, to check inoculum purity.
4. the preparation and bacterium solution inoculation of antibacterials are diluted
Take sterile test tube（13x100mm）13, it is arranged in a row, in addition to the 1st pipe adds 1.6mlMH meat soups, remaining often pipe addition MH
Meat soup 1ml, in the 1st pipe Formulas I antibacterials stock solution is added（Such as 1150 μ g/ml）0.4ml is mixed, and is then drawn 1ml to the 2nd and is managed,
Draw the pipes of 1ml to the 3rd after mixing again, so continuous doubling dilution is to the 11st pipe, and absorption 1ml is discarded from the 11st pipe, the 12nd
Manage as the growth control of not drug containing.Now each pipe drug level be followed successively by 256,128,64,32,16,8,4,2,1,0.5,
0.25μg/ml.Then each 1ml of the above-mentioned inoculum for preparing is added in every pipe, make the final bacterial concentration of every pipe be about 5 ×
105CFU/ml.1st pipe is respectively 128,64,32,16,8,4,2,1,0.5,0.25,0.125 μ g/ to the 11st pipe drug level
Ml. equally, Formula II oligomeric phenolic compound and positive control vancomycin are as above equally operated.By the dilution pipe close being inoculated with
Good stopper incubation, puts and be incubated in 35 DEG C of normal air incubators full 24h.
5. result judges and explains
Before the MIC of reading and report institute test strain, should check whether the bacterial growth situation of growth control pipe is good, while
The Secondary Culture situation of inoculum should also be checked to determine if pollution, whether the MIC value of Quality-control strains is in Quality Control scope.
With perusal, medicine least concentration pipe is without bacterial growth person, the MIC of as tested bacterium.Compare suppression with Growth positive control tube
80% bacterial growth pipe drug level is made for tested bacterium MIC value.
The present embodiment two compound antimicrobial agent MIC value test results of gained are as shown in table 1.
Embodiment 3. extracts compound I and II from paeonia rockii seed and antimicrobial agent is determined
（1）The extraction of compound I and II：
7 kilograms of Paeonia ostii Paeonia suffruticosa seed is purchased, shelling is dried.Seed of Flos Moutan shell is crushed and is soaked with 70% alcohol at normal temperature, continue 3 days, repeated
Twice.By the leachate vacuum distillation for obtaining, the g of ethanol extract 400 is obtained.300 g ethanol extracts are taken, by ethanol extract with molten
After agent dissolving, take after macroporous resin decolourizes, then with 800 g silica gel（100-200 mesh）Mix sample to load.First with 5 times of column volumes
Ethyl acetate carries out eluting, then carries out eluting with the acetone of 5 times of column volumes.Eluent does respectively vacuum distillation, obtains acetic acid
The g of ethyl ester extractum 110 and the g extractum of acetone 150.
Acetone extract is taken, by Waters high performance liquid preparative chromatographies（Methanol：Water 55:45, flow velocity 8ml/min, YMC
ODS C18,250*20mm）, component F1 is obtained, F2, F3 and F4. component F1 passes through Waters high performance liquid chromatography（Methanol：Water
50:50, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound II Radix Gentianae H（Gnetin H）；Component F3 passes through
Waters high performance liquid chromatography（Methanol：Water 45:55, flow velocity 8ml/min, YMC ODS C18,250*20mm）, obtain compound I
Trans-D- grape elements（Trans-ε-Viniferin）.
（2）The antimicrobial agent of compound I and II is determined：
1. prepared by antibacterials stock solution
Positive control antibiotic vancomycin and all bacterial strains, directly grind purchased from Beijing Chinese Academy of Medical Sciences medical biotechnology
Study carefully institute.
Concrete configuration refers to NCCLS sensitivity testing to antibacterials operation standards, tested antibacterials Formulas I and Formula II two
Oligomeric phenolic configuration concentration is not lower than 1000 μ g/mL.Such as need to prepare the antibiotic storage that 100 mL concentration are 1150 μ g/mL
Liquid, sets the effective force of its medicine as 750 μ g/mg.The amount for accurately weighing antibiotic powder with analytical balance is 164.1 μ g.Root
Calculating required diluent consumption according to formula is：（164.1μg×750μg/mL）/ 1150 μ g/mL=107.0mL, then by 164.1 μ
G antibiotic powders are dissolved in 107.0mL diluent.The antibacterials stock solution for preparing should be stored in less than -60 DEG C environment,
Storage life was less than 4-6 month.
2. prepared by drug sensitive test culture medium
According to NCCLS sensitivity testing to antibacterials operation standards, culture medium uses Mueller-Hinton（MH）Meat soup,
pH7.2~7.4.In addition 2% is added in meat soup（W/V）Sodium Chloride.The MH meat soups of requirement are prepared by the requirement of manufacturing firm.
3. the preparation of inoculum adopts direct bacterium colony suspension preparation method.Methicillin-resistant Staphylococcus directly take culture 18 ~
24h.Take 1-2 methicillin-resistant Staphylococcus bacterium colony to put in above-mentioned 2 culture fluid, be deployed into 0.5 Maxwell than turbid standard
Bacteria suspension.
Take MH meat soups by above-mentioned bacteria suspension carry out 1: 1 dilution after it is standby.Note to be inoculated with 15 minutes and prepare
Inoculum, and a inoculum Secondary Culture on non-selective agar flat board is taken, to check inoculum purity.
4. the preparation and bacterium solution inoculation of antibacterials are diluted
Take sterile test tube（13x100mm）13, it is arranged in a row, in addition to the 1st pipe adds 1.6mlMH meat soups, remaining often pipe addition MH
Meat soup 1mLl, in the 1st pipe Formulas I antibacterials stock solution is added（Such as 1150 μ g/mL）0.4ml is mixed, and is then drawn 1mL to the 2nd and is managed,
Draw the pipes of 1mL to the 3rd after mixing again, so continuous doubling dilution is to the 11st pipe, and absorption 1mL is discarded from the 11st pipe, the 12nd
Manage as the growth control of not drug containing.Now each pipe drug level be followed successively by 256,128,64,32,16,8,4,2,1,0.5,
0.25μg/mL.Then each 1ml of the above-mentioned inoculum for preparing is added in every pipe, make the final bacterial concentration of every pipe be about 5 ×
105CFU/mL.1st pipe is respectively 128,64,32,16,8,4,2,1,0.5,0.25,0.125 μ g/ to the 11st pipe drug level
ML. equally, Formula II oligomeric phenolic compound and positive control vancomycin are as above equally operated.By the dilution pipe close being inoculated with
Good stopper incubation, puts and be incubated in 35 DEG C of normal air incubators full 24h.
5. result judges and explains
Before the MIC of reading and report institute test strain, should check whether the bacterial growth situation of growth control pipe is good, while
The Secondary Culture situation of inoculum should also be checked to determine if pollution, whether the MIC value of Quality-control strains is in Quality Control scope.
With perusal, medicine least concentration pipe is without bacterial growth person, the MIC of as tested bacterium.Compare suppression with Growth positive control tube
80% bacterial growth pipe drug level is made for tested bacterium MIC value.
The present embodiment two compound antimicrobial agent MIC value test results of gained are as shown in table 1.
Claims (4)
1. oligomeric polyphenol compound is preparing the application of resisting gram-positive fastbacteria product, it is characterised in that：Described is low
Poly- polyphenol compound is the dimer and the oligomeric polyphenol compound of trimer of transconfiguration.
2. oligomeric polyphenol compound as claimed in claim 1 is in the application for preparing resisting gram-positive fastbacteria product, institute
The oligomeric polyphenol compound of dimer and trimer stated is compound 1：Trans-D- grape elements and compound 2：Radix Gentianae H, structure
Formula is respectively：
Compound 1
Compound 2.
3. oligomeric polyphenol compound as claimed in claim 1 is in the application for preparing resisting gram-positive fastbacteria product, its
It is characterised by：Described dimer antimicrobial agent is higher compared with the antimicrobial agent of trimer.
4. oligomeric polyphenol compound as claimed in claim 3 is in the application for preparing resisting gram-positive fastbacteria product, its
It is characterised by：The antimicrobial agent MIC value of the trans-D- grape elements of described dimer be 8 μ g/mL, the overriding resistance of trimer Radix Gentianae H
Bacterium MIC value is 16 μ g/mL.
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| CN113015809A (en) * | 2018-11-13 | 2021-06-22 | 韩国生命工学研究院 | Method for producing stilbene dimer by using plant callus culture solution |
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| CN105325702A (en) * | 2015-11-30 | 2016-02-17 | 河南科技大学 | Gentian H-containing natural antibiotic feed additive as well as preparation and application thereof |
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|---|---|---|---|---|
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