CN106665347A - Detoxification and rapid propagation method of sweet potatoes of Yizi 138 - Google Patents
Detoxification and rapid propagation method of sweet potatoes of Yizi 138 Download PDFInfo
- Publication number
- CN106665347A CN106665347A CN201610632415.XA CN201610632415A CN106665347A CN 106665347 A CN106665347 A CN 106665347A CN 201610632415 A CN201610632415 A CN 201610632415A CN 106665347 A CN106665347 A CN 106665347A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- detoxification
- word
- stem
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a detoxification and rapid propagation method of sweet potatoes of Yizi 138. The detoxification and rapid propagation method comprises the following specific steps: treating stem tips at the top of the sweet potato of Yizi 138 to obtain a stem tip meristematic tissue; inoculating the stem tip meristematic tissue to a stem tip induction medium for culture to obtain a stem tip test-tube plantlet; firstly weeding out weak seedlings and symptomatic appearance seedlings through a visual observation method, then authenticating sweet potato viruses by a serological method, and weeding out non-detoxified sweet potato seedlings of Yizi 138; under a germ-free condition, cutting the high-quality sweet potato seedling stem tips subjected to stem tip meristematic tissue culture and detoxification authentication into single sections, inoculating the single sections into a common culture medium for continuous switching culture, and carrying out rapid propagation on the detoxified sweet potato seedlings of Yizi 138. According to the simple and feasible detoxification and rapid propagation method of the sweet potatoes of Yizi 138, an extremely good detoxification effect can be achieved; a large quantity of high-quality seedlings are provided; the high-quality seedlings and healthy seedlings are used for rapid propagation, so that the planting benefit can be increased, and most farmers and development enterprises are benefited.
Description
Technical field
The present invention relates to Sweetpotato Viruses Elimination field, more particularly to a kind of detoxifying fast breeding method for losing the sweet potato of word 138.
Background technology
Lose the sweet potato food flavor of word 138 good, sugar content is high, glue that softness is good, be that the both urban and rural residents likes one of edible kind.It is fitted
Preferably cultivate as spring, summer potato, preferably, block number is more, and potato wedges size is more uniform moderate for resistance to fertilizer, waterlogging tolerance.The sweet potato of word 138 is lost with potato
The nutrition organs such as block and vines carries out vegetative propagation, introducing a fine variety, breed and production process in easily cause the accumulation of Virus Diseases of Sweet Potato
With spread, so as to cause yield reduction, quality decline, plant sexual involution the problems such as.Hence with health seedling carry out it is fast it is numerous just into
For inevitable choice, but general poison-removing method detoxification efficiency is not it is obvious that not only expend raw material, to have an effect on income.
The content of the invention
Present invention seek to address that the deficiencies in the prior art, and a kind of detoxifying fast breeding method for losing the sweet potato of word 138 is provided.
The present invention for achieving the above object, is employed the following technical solutions：
A kind of detoxifying fast breeding method for losing the sweet potato of word 138, concretely comprises the following steps：
(1) acquisition of aseptic explant：
Choose the stem apex for losing word 138 sweet potato seedling top 1.5cm length, first with 70% alcohol-pickled 10-15s, then with 0.1%
Mercury chloride sterilization 6-8min, then used aseptic water washing 6-8 time, on superclean bench, entered using dissection sem observation
The stripping of row stem apex, obtains the shoot apical meristem of 0.3-0.4mm；
(2) stem-apex Meristem culture detoxification：
The shoot apical meristem for peeling off acquisition is seeded on Stem tip induction culture medium, temperature is statically placed in for 30 DEG C, illumination
Intensity is under conditions of 3000lx, 18h illumination cultivations to be carried out daily, and incubation time is 18-25 days, is proceeded to ordinary culture medium
On, 15h illumination cultivations are carried out daily, incubation time is 55-60 days, obtains stem apex test tube seedling；
(3) detoxification identification：
Ocular estimate is taken first, weak seedling and aobvious disease seedling is eliminated, and is then eliminated not using serological method identification sweet potato viruses again
There is the sweet potato seedling of something lost word 138 of detoxification；
(4) Virus-free Sweetpotato seedling is numerous soon：
Aseptically, the high-quality after stem-apex Meristem culture and detoxification identification is lost into the sweet potato seedling stem of word 138
Point, is cut into single-unit and is inoculated on ordinary culture medium, is statically placed in temperature for 30 DEG C, and intensity of illumination is under conditions of 3000lx, daily
Carry out 15h illumination cultivations, incubation time is 20-23 days, constantly transfer culture by this program, carry out the detoxification something lost sweet potato of word 138
Seedling it is fast numerous.
The Stem tip induction culture medium consists of the agar+1mg/L6-BA of MS+3% sucrose+0.7%.
The ordinary culture medium consists of the agar of MS+3% sucrose+0.7%.
The serological method identifies that the concrete operation method for losing the sweet potato viruses of word 138 is：Base portion 2-3 piece leaves are taken per test tube seedling
In being fitted into polybag, 1mlTBS-DIECA Extraction buffers are added, gently rolled, sample is mixed with tissue fluid, stood at 4 DEG C
70-80min, takes supernatant liquid spotting on ready celluloid sample film, carries out dot enzyme-linked immuno adsorption experiment, then
Rinse, colour developing, if present bluish violet be without detoxification, it is colourless to slough virus.
The invention has the beneficial effects as follows：The invention provides a kind of simple fast numerous side of something lost word 138 Sweetpotato Viruses Elimination
Method, can reach good detoxification efficiency, there is provided substantial amounts of high quality seedling, carried out using this high-quality health seedling it is fast numerous,
Plantation income can be improved, vast Planting household and exploitation enterprise is benefited.
Specific embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1：
A kind of detoxifying fast breeding method for losing the sweet potato of word 138, concretely comprises the following steps：
(1) acquisition of aseptic explant：
Choose the stem apex for losing word 138 sweet potato seedling top 1.5cm length, first with 70% alcohol-pickled 10s, then with 0.1%
Mercury chloride is sterilized 8min, is then used aseptic water washing 8 times, on superclean bench, using sem observation is dissected stem apex is carried out
Stripping, obtain 0.3mm shoot apical meristem；
(2) stem-apex Meristem culture detoxification：
The shoot apical meristem for peeling off acquisition is seeded on Stem tip induction culture medium, temperature is statically placed in for 30 DEG C, illumination
Intensity is under conditions of 3000lx, 18h illumination cultivations to be carried out daily, and incubation time is 18 days, is proceeded on ordinary culture medium,
15h illumination cultivations are carried out daily, and incubation time is 60 days, obtains stem apex test tube seedling；
(3) detoxification identification：
Ocular estimate is taken first, weak seedling and aobvious disease seedling is eliminated, and is then eliminated not using serological method identification sweet potato viruses again
There is the sweet potato seedling of something lost word 138 of detoxification；
(4) Virus-free Sweetpotato seedling is numerous soon：
Aseptically, the high-quality after stem-apex Meristem culture and detoxification identification is lost into the sweet potato seedling stem of word 138
Point, is cut into single-unit and is inoculated on ordinary culture medium, is statically placed in temperature for 30 DEG C, and intensity of illumination is under conditions of 3000lx, daily
Carry out 15h illumination cultivations, incubation time is 23 days, constantly transfer culture by this program, carry out the detoxification something lost sweet potato seedling of word 138
It is fast numerous.
The Stem tip induction culture medium consists of the agar+1mg/L6-BA of MS+3% sucrose+0.7%.
The ordinary culture medium consists of the agar of MS+3% sucrose+0.7%.
The serological method identifies that the concrete operation method for losing the sweet potato viruses of word 138 is：2 leaf dresses of base portion are taken per test tube seedling
In entering polybag, 1mlTBS-DIECA Extraction buffers are added, gently rolled, sample is mixed with tissue fluid, stood at 4 DEG C
70min, takes supernatant liquid spotting on ready celluloid sample film, carries out dot enzyme-linked immuno adsorption experiment, Ran Houchong
Wash, develop the color, if present bluish violet be without detoxification, it is colourless to slough virus.
The invention provides a kind of simple fast numerous method of something lost word 138 Sweetpotato Viruses Elimination, can reach de- well
Toxic effect fruit, there is provided substantial amounts of high quality seedling, is carried out fast numerous, it is possible to increase plantation income, favour using this high-quality health seedling
And vast Planting household and exploitation enterprise.
Embodiment 2：
A kind of detoxifying fast breeding method for losing the sweet potato of word 138, concretely comprises the following steps：
(1) acquisition of aseptic explant：
Choose the stem apex for losing word 138 sweet potato seedling top 1.5cm length, first with 70% alcohol-pickled 15s, then with 0.1%
Mercury chloride is sterilized 6min, is then used aseptic water washing 6 times, on superclean bench, using sem observation is dissected stem apex is carried out
Stripping, obtain 0.4mm shoot apical meristem；
(2) stem-apex Meristem culture detoxification：
The shoot apical meristem for peeling off acquisition is seeded on Stem tip induction culture medium, temperature is statically placed in for 30 DEG C, illumination
Intensity is under conditions of 3000lx, 18h illumination cultivations to be carried out daily, and incubation time is 25 days, is proceeded on ordinary culture medium,
15h illumination cultivations are carried out daily, and incubation time is 55 days, obtains stem apex test tube seedling；
(3) detoxification identification：
Ocular estimate is taken first, weak seedling and aobvious disease seedling is eliminated, and is then eliminated not using serological method identification sweet potato viruses again
There is the sweet potato seedling of something lost word 138 of detoxification；
(4) Virus-free Sweetpotato seedling is numerous soon：
Aseptically, the high-quality after stem-apex Meristem culture and detoxification identification is lost into the sweet potato seedling stem of word 138
Point, is cut into single-unit and is inoculated on ordinary culture medium, is statically placed in temperature for 30 DEG C, and intensity of illumination is under conditions of 3000lx, daily
Carry out 15h illumination cultivations, incubation time is 20 days, constantly transfer culture by this program, carry out the detoxification something lost sweet potato seedling of word 138
It is fast numerous.
The Stem tip induction culture medium consists of the agar+1mg/L6-BA of MS+3% sucrose+0.7%.
The ordinary culture medium consists of the agar of MS+3% sucrose+0.7%.
The serological method identifies that the concrete operation method for losing the sweet potato viruses of word 138 is：3 leaf dresses of base portion are taken per test tube seedling
In entering polybag, 1mlTBS-DIECA Extraction buffers are added, gently rolled, sample is mixed with tissue fluid, stood at 4 DEG C
80min, takes supernatant liquid spotting on ready celluloid sample film, carries out dot enzyme-linked immuno adsorption experiment, Ran Houchong
Wash, develop the color, if present bluish violet be without detoxification, it is colourless to slough virus.
The invention provides a kind of simple fast numerous method of something lost word 138 Sweetpotato Viruses Elimination, can reach de- well
Toxic effect fruit, there is provided substantial amounts of high quality seedling, is carried out fast numerous, it is possible to increase plantation income, favour using this high-quality health seedling
And vast Planting household and exploitation enterprise.
Embodiment 3：
A kind of detoxifying fast breeding method for losing the sweet potato of word 138, concretely comprises the following steps：
(1) acquisition of aseptic explant：
Choose the stem apex for losing word 138 sweet potato seedling top 1.5cm length, first with 70% alcohol-pickled 13s, then with 0.1%
Mercury chloride is sterilized 7min, is then used aseptic water washing 7 times, on superclean bench, using sem observation is dissected stem apex is carried out
Stripping, obtain 0.3mm shoot apical meristem；
(2) stem-apex Meristem culture detoxification：
The shoot apical meristem for peeling off acquisition is seeded on Stem tip induction culture medium, temperature is statically placed in for 30 DEG C, illumination
Intensity is under conditions of 3000lx, 18h illumination cultivations to be carried out daily, and incubation time is 22 days, is proceeded on ordinary culture medium,
15h illumination cultivations are carried out daily, and incubation time is 57 days, obtains stem apex test tube seedling；
(3) detoxification identification：
Ocular estimate is taken first, weak seedling and aobvious disease seedling is eliminated, and is then eliminated not using serological method identification sweet potato viruses again
There is the sweet potato seedling of something lost word 138 of detoxification；
(4) Virus-free Sweetpotato seedling is numerous soon：
Aseptically, the high-quality after stem-apex Meristem culture and detoxification identification is lost into the sweet potato seedling stem of word 138
Point, is cut into single-unit and is inoculated on ordinary culture medium, is statically placed in temperature for 30 DEG C, and intensity of illumination is under conditions of 3000lx, daily
Carry out 15h illumination cultivations, incubation time is 21 days, constantly transfer culture by this program, carry out the detoxification something lost sweet potato seedling of word 138
It is fast numerous.
The Stem tip induction culture medium consists of the agar+1mg/L6-BA of MS+3% sucrose+0.7%.
The ordinary culture medium consists of the agar of MS+3% sucrose+0.7%.
The serological method identifies that the concrete operation method for losing the sweet potato viruses of word 138 is：3 leaf dresses of base portion are taken per test tube seedling
In entering polybag, 1mlTBS-DIECA Extraction buffers are added, gently rolled, sample is mixed with tissue fluid, stood at 4 DEG C
75min, takes supernatant liquid spotting on ready celluloid sample film, carries out dot enzyme-linked immuno adsorption experiment, Ran Houchong
Wash, develop the color, if present bluish violet be without detoxification, it is colourless to slough virus.
The invention provides a kind of simple fast numerous method of something lost word 138 Sweetpotato Viruses Elimination, can reach de- well
Toxic effect fruit, there is provided substantial amounts of high quality seedling, is carried out fast numerous, it is possible to increase plantation income, favour using this high-quality health seedling
And vast Planting household and exploitation enterprise.
The present invention is exemplarily described above, it is clear that the present invention is implemented and is not subject to the restrictions described above,
As long as employing method of the present invention design and the various improvement that carry out of technical scheme, or not improved directly apply to other fields
Close, within protection scope of the present invention.
Claims (4)
1. it is a kind of lose the sweet potato of word 138 detoxifying fast breeding method, it is characterised in that concretely comprise the following steps：
(1) acquisition of aseptic explant：
Choose the stem apex for losing word 138 sweet potato seedling top 1.5cm length, first with 70% alcohol-pickled 10-15s, then with 0.1% chlorine
Change mercury sterilization 6-8min, then used aseptic water washing 6-8 time, on superclean bench, stem is carried out using dissection sem observation
The stripping of point, obtains the shoot apical meristem of 0.3-0.4mm；
(2) stem-apex Meristem culture detoxification：
The shoot apical meristem for peeling off acquisition is seeded on Stem tip induction culture medium, temperature is statically placed in for 30 DEG C, intensity of illumination
Under conditions of for 3000lx, 18h illumination cultivations are carried out daily, incubation time is 18-25 days, is proceeded on ordinary culture medium,
15h illumination cultivations are carried out daily, and incubation time is 55-60 days, obtains stem apex test tube seedling；
(3) detoxification identification：
Ocular estimate is taken first, weak seedling and aobvious disease seedling is eliminated, and is then eliminated without de- using serological method identification sweet potato viruses again
The sweet potato seedling of something lost word 138 of poison；
(4) Virus-free Sweetpotato seedling is numerous soon：
Aseptically, the high-quality after stem-apex Meristem culture and detoxification identification is lost into the sweet potato seedling stem apex of word 138, is cut
It is inoculated on ordinary culture medium into single-unit, is statically placed in temperature for 30 DEG C, intensity of illumination is under conditions of 3000lx, to carry out daily
15h illumination cultivations, incubation time is 20-23 days, is constantly transferred culture by this program, carries out the detoxification something lost sweet potato seedling of word 138
It is fast numerous.
2. it is according to claim 1 it is a kind of lose the sweet potato of word 138 detoxifying fast breeding method, it is characterised in that the stem apex is lured
That leads culture medium consists of the agar+1mg/L6-BA of MS+3% sucrose+0.7%.
3. it is according to claim 2 it is a kind of lose the sweet potato of word 138 detoxifying fast breeding method, it is characterised in that the common training
Foster base consists of the agar of MS+3% sucrose+0.7%.
4. it is according to claim 3 it is a kind of lose the sweet potato of word 138 detoxifying fast breeding method, it is characterised in that the serology
Method identifies that the concrete operation method for losing the sweet potato viruses of word 138 is：Base portion 2-3 piece leaves are taken per test tube seedling to be fitted into polybag, add
1mlTBS-DIECA Extraction buffers, gently roll, and sample is mixed with tissue fluid, and at 4 DEG C 70-80min is stood, and take supernatant
Point sample carries out dot enzyme-linked immuno adsorption experiment on ready celluloid sample film, then rinses, develops the color, if being in
Existing bluish violet be without detoxification, it is colourless to slough virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610632415.XA CN106665347A (en) | 2016-08-03 | 2016-08-03 | Detoxification and rapid propagation method of sweet potatoes of Yizi 138 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610632415.XA CN106665347A (en) | 2016-08-03 | 2016-08-03 | Detoxification and rapid propagation method of sweet potatoes of Yizi 138 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106665347A true CN106665347A (en) | 2017-05-17 |
Family
ID=58839295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610632415.XA Pending CN106665347A (en) | 2016-08-03 | 2016-08-03 | Detoxification and rapid propagation method of sweet potatoes of Yizi 138 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106665347A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810247A (en) * | 2019-12-05 | 2020-02-21 | 天津丰华裕隆农业发展股份有限公司 | Sweet potato stem tip detoxification and breeding method |
| CN114051927A (en) * | 2021-11-09 | 2022-02-18 | 延安市农业科学研究所 | Simplified breeding method for virus-free sweet potato seedlings |
-
2016
- 2016-08-03 CN CN201610632415.XA patent/CN106665347A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810247A (en) * | 2019-12-05 | 2020-02-21 | 天津丰华裕隆农业发展股份有限公司 | Sweet potato stem tip detoxification and breeding method |
| CN114051927A (en) * | 2021-11-09 | 2022-02-18 | 延安市农业科学研究所 | Simplified breeding method for virus-free sweet potato seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Singh et al. | Micropropagation for production of quality banana planting material in Asia-Pacific | |
| Pornpienpakdee et al. | Improving the micropropagation efficiency of hybrid Dendrobium orchids with chitosan | |
| CN105145359B (en) | Tissue culture and rapid propagation method for asparagus filicinus | |
| CN106069753A (en) | A kind of Bougainvillea spectabilis aseptic seeding and method for quickly breeding | |
| CN107047320A (en) | A kind of bigflower centranthera root method for tissue culture | |
| Rafiq et al. | Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv.‘Ravenna’ | |
| CN103651111B (en) | Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method | |
| CN108522275A (en) | A kind of disease-resistant homozygotic breeding method of tomato | |
| CN105340741B (en) | A method of improving radix stemonae tuberosae tissue-cultured seedling rooting rate | |
| Jain et al. | Regeneration ofjuvenile'plants of black plum, Syzygium cuminii Skeels, from nodal exp of mature trees | |
| Iqbal et al. | Optimization of in vitro micropropagation protocol for banana (Musa sapientum L.) under different hormonal concentrations and growth media. | |
| Shahriyar et al. | In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration | |
| Parab et al. | Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack | |
| Patil et al. | In vitro micropropagation of Lilium candidum bulb by application of multiple hormone concentrations using plant tissue culture technique | |
| CN106665347A (en) | Detoxification and rapid propagation method of sweet potatoes of Yizi 138 | |
| CN108901858A (en) | A kind of phoenix head ginger selenium-rich original silkworm egg quick-breeding method | |
| Chamandoosti | Citrus tissue culture with two different approaches | |
| CN104823849A (en) | Rapid propagation method of cassava virus-free seedlings | |
| CN104521758B (en) | A kind of Xian Jing hand over gene introgression blast resisting breeding new method | |
| Manawadu et al. | Colchicine induced tetraploids of radish (Raphanus sativus L.). | |
| CN106386494B (en) | A kind of sweet potato stem tip detoxification and breeding method | |
| CN104429958A (en) | Method for in-vitro rejuvenation of ulmus pumila tissue culture seedlings | |
| CN108056021A (en) | A kind of purslane callus induction and the method for producing flavonoids | |
| CN107439211A (en) | A kind of method for shortening the eggplant budding seedling time | |
| Verma et al. | Standardization of protocol for pre-treatment, surface sterilization, regeneration, elongation and acclimatization of Chrysanthemum morifolium Ramat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170517 |
|
| WD01 | Invention patent application deemed withdrawn after publication |