CN106645492A - Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition - Google Patents
Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition Download PDFInfo
- Publication number
- CN106645492A CN106645492A CN201611241605.5A CN201611241605A CN106645492A CN 106645492 A CN106645492 A CN 106645492A CN 201611241605 A CN201611241605 A CN 201611241605A CN 106645492 A CN106645492 A CN 106645492A
- Authority
- CN
- China
- Prior art keywords
- animal
- real
- monoamine neurotransmitter
- time
- exposure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
Abstract
A method for real-time detection of monoamine neurotransmitter change in brains of animals under a smoke exposure condition is characterized by exposing the animals to smoke in real time by adopting an animal oro-nasal inhalation smoke exposure system, collecting an animal brain tissue sample through an on-line microdialysis technique, and quickly analyzing monoamine neurotransmitters and metabolite thereof in a brain dialysate sample by using a high performance liquid chromatography/coularray detector, so as to detect monoamine neurotransmitter change characteristics in the brains of the animals before and after smoke exposure in real time. According to the method provided by the invention, exposure to the smoke of the animals is more similar to the manner of human beings smoking tobacco products, the technical difficulty of incapability of real-time simulating and evaluating of monoamine neurotransmitter change caused by smoke inhaling (nicotine intaking) is overcome, the method has the advantages of simple operation, accurate and reliable result and the like, and compared with the prior art, a new method for monitoring monoamine neurotransmitter metabolic change in the brains of the animals after smoke exposure is created.
Description
Technical field
The present invention relates under the conditions of real-time detection fume exposure animal intracerebral monoamine neurotransmitter mutation analysis method,
This method collects the animal used as test brain tissue sample being exposed in real time in flue gas by on-line microdialysis technology, recycles efficient liquid
Phase chromatogram-coulomb array electrochemical detector is carried out quickly to the monoamine neurotransmitter and its metabolin in brain dialysate samples
On-line analysis, so as to Real-Time Evaluation flue gas suck exposure before and after monoamine neurotransmitter variation characteristic.
Background technology
Key character composition --- the nicotine of cigarette smoke and electronics smoke sol all containing tobacco（Nicotine）.Nicotine
Dependence is the main cause of smoking addiction, is also the basic reason of lasting smoking tobaccos product and then health risk.Take the photograph
Enter internal nicotine through blood-brain barrier and can be distributed widely in each region of brain tissue rapidly, and it is many that it mainly acts on middle limbic brain
Bar amine（Dopamine, DA）System and produce rewarding effect.In fact, including DA, serotonin（5-HT）Deng monoamine nerve
Mediator has been involved in the generating process of nicotine Neuropharmacology effect.Therefore, developing a kind of new detection method can be to flue gas
The release of the animal intracerebral monoamine neurotransmitter that exposure causes and metabolic alterations carry out Accurate Determining and Real-Time Evaluation, will be to take off
Show that the genesis mechanism of smoking addiction provides important means and supports.
HPLC-electrochemical detection being adopted in biological sample the measure of monoamine neurotransmitter more（HPLC-
ECD）, high selectivity that wherein high performance liquid chromatography-coulomb array electrochemical detector presents because its multichannel is responded and
High sensitivity, using relatively broad.It is combined using on-line microdialysis technology (Online microdialysis) and HPLC-ECD
Live body, the dynamic measurement to monoamine neurotransmitters such as DA, 5-HT can be realized, but the method is only capable of evaluating medicine exposure
（Such as nicotine injection）The monoamine neurotransmitters change for causing afterwards；Suction flue gas is integrally exposed using animal（By animal
It is placed in closed flue gas cabin）Exposure chamber cannot also synchronously complete flue gas in real time exposure and animal intracerebral monoamine nerve are passed
The on-line determination of matter.
The content of the invention
The present invention is exactly for cannot synchronously realize at present flue gas suction exposure and monoamine neurotransmitter on-line determination
Deficiency, and the analysis side that animal intracerebral monoamine neurotransmitter changes under the conditions of a kind of real-time monitoring fume exposure specially developed
Method.This method realizes animal mouth and nose formula fume exposure system by using improved animal used as test nose suction exposure clamper
The combination of system and Microdialysis sgstem, and then by using on-line microdialysis and high performance liquid chromatography-coulomb array electrochemical detection
Device GC-MS realizes the Real-time Collection and on-line determination that exposure animal used as test brain tissue sample is sucked to flue gas, has reached cigarette
Aspiration enters the technical requirements that exposure is synchronously carried out with intracerebral monoamine neurotransmitter change detection.In the method, animal used as test
Employ only mouth and nose suction（nose-only）Mode be exposed in flue gas, be closer to the side of mankind's smoking tobaccos product
Formula, overcoming cannot the suction of Real-Time Evaluation flue gas（Nicotine is taken in）The technical barrier of the monoamine neurotransmitter change for causing, has
Easy to operate, result accurately and reliably the advantages of.
The purpose of the present invention is achieved through the following technical solutions：Animal under the conditions of a kind of real-time detection fume exposure
The method of intracerebral monoamine neurotransmitter change, animal is exposed in real time cigarette using animal mouth and nose formula suction fume exposure system
In gas, animal brain's sample is collected by on-line microdialysis technology, recycle high performance liquid chromatography-coulomb array electrochemical inspection
Survey device quickly to analyze the monoamine neurotransmitter and its metabolin in brain dialysate samples, so as to real-time monitoring flue gas is sudden and violent
The variation characteristic of animal intracerebral monoamine neurotransmitter before and after dew, and then according to the analysis of DA, 5-HT in sample and its metabolin
The intracerebral monoamine neurotransmitter Changing Pattern that testing result Real-Time Evaluation fume exposure causes.
The method is comprised the following steps that：
A. fume exposure：Electronic cigarette liquid is set to produce aerosol using smoking machine smoking cigarette or using aerosol generator, by head
Animal equipped with microdialysis probe conduit is put into a special animal used as test geometrical clamp holder in portion, so that the clamper seizes dynamic on both sides by the arms
Thing is only exposed in certain density flue gas with mouth and nose suction；
B. encephalic micro-dialysis sample collection：Microdialysis probe is inserted continuous in the conduit of animal head after operation Microdialysis sgstem
Animal brain dislysate is collected in the quantitative loop of online injector, at regular intervals operation is automatically injected by online injector
High performance liquid chromatography in.
C. high performance liquid chromatography-coulomb array electrochemical detector analysis：Setting gradient elution program and sample introduction sequence are simultaneously
Operation high performance liquid chromatography-coulomb array electrochemical detector detection microdialysis sample so that the detection time of each sample is omited
Less than the acquisition time of the sample.
D. standard liquid is prepared and Specification Curve of Increasing：DA, 3,4 dihydroxyphenyl acetic acids (DOPAC), high-quality slender joss stick is weighed respectively
Oxalic acid (HVA), 5-HT and each 10mg of 5 hydroxyindole acetic acids (5-HIAA), 5 kinds of standard items, in being respectively placed in 100mL volumetric flasks, use
Methanol/water solution（20:80, volume ratio, similarly hereinafter）Scale is dissolved and be diluted to, is shaken up, it is respectively the standard of 100 μ g/mL to obtain concentration
Stock solution；The Standard Stock solutions of 1.0mL are pipetted respectively, in being placed in same 100mL volumetric flask, are diluted to methanol/water
Scale, shakes up, and it is respectively the one-level mixed standard solution of 1.0 μ g/mL to obtain concentration；Accurately pipette 0.01,0.05,0.10,0.50,
1.00th, 5.00,10.0 and 50.0mL one-level mixed standard solution, in being respectively placed in different 100mL volumetric flasks, uses compound chlorination
Sodium injection is diluted to scale, shakes up, and obtains concentration equal 0.1,0.5,1.0,5.0,10.0,50.0,100.0 and 500.0ng/mL
Serial mixed standard solution.Serial mixed standard solution is carried out into high performance liquid chromatography-storehouse by concentration order from low to high
Logical sequence array electrochemical detector is analyzed, with the chromatographic peak area of each target analytes（Y）To its corresponding concentration（X）Carry out linear
Return, obtain calibration curve regression equation.
E. data analysis and process：The content of each object in dialysate samples is calculated according to calibration curve method, according to knot
Fruit draws respectively the forward and backward each time point intracerebral monoamine neurotransmitter of fume exposure and its Concentration-time change of metabolin is bent
Line, discloses the rule that fume exposure causes intracerebral monoamine neurotransmitter to change.
One aspect of the present invention utilizes animal mouth and nose formula fume exposure system, can be being closer to mankind's smoking tobaccos product
Mode makes animal be exposed to by way of only nasal inhalation in flue gas, on the other hand also using on-line microdialysis and high-efficient liquid phase color
Spectrum-coulomb array electrochemical detector GC-MS, can not only in real time obtain the animal brain sample being exposed in flue gas simultaneously real
Existing on-line checking monoamine neurotransmitter, while the features such as also there is high sensitivity, short sampling interval time and fast analyze speed.
5 kinds of monoamine neurotransmitter compounds of animal intracerebral can not only be simultaneously detected using technical scheme, is also achieved
Online, the dynamic measurement for causing animal intracerebral monoamine neurotransmitter to change during fume exposure, achieves compared with prior art
It is substantially progressive, start a kind of new monitoring side for animal intracerebral Monoamine Metabolism change after fume exposure
Method.
Description of the drawings
Fig. 1 is test mode schematic diagram of the present invention.
Sequence number in Fig. 1：1 is microdialysis infusion pump, and 2 is only to pass through the animal that mouth and nose formula sucks flue gas in clamper, 3
For microdialysis probe, 4 is on-line automatic injector, and 5 is high performance liquid chromatography-coulomb array electrochemical detector system）.
Fig. 2 is the HPLC-ECD detection collection of illustrative plates of 5 kinds of objects in rat encephalic micro-dialysis sample；
In figure：a. DA；b. 5-HT；c. DOPAC；d. 5-HIAA；e. HVA.
Fig. 3 is the Concentration-time changing trend diagram of rat intracerebral DA before and after cigarette smoke exposure.
Fig. 4 is the Concentration-time changing trend diagram of rat intracerebral DOPAC before and after cigarette smoke exposure.
Fig. 5 is the Concentration-time changing trend diagram of rat intracerebral HVA before and after cigarette smoke exposure.
Fig. 6 is the Concentration-time changing trend diagram of rat intracerebral 5-HT before and after cigarette smoke exposure.
Fig. 7 is the Concentration-time changing trend diagram of rat intracerebral 5-HIAA before and after cigarette smoke exposure.
Fig. 8 is the Concentration-time changing trend diagram of rat intracerebral DA before and after electronic cigarette fume exposure.
Fig. 9 is the Concentration-time changing trend diagram of rat intracerebral DOPAC before and after electronic cigarette fume exposure.
Figure 10 is the Concentration-time changing trend diagram of rat intracerebral HVA before and after electronic cigarette fume exposure.
Figure 11 is the Concentration-time changing trend diagram of rat intracerebral 5-HT before and after electronic cigarette fume exposure.
Figure 12 is the Concentration-time changing trend diagram of rat intracerebral 5-HIAA before and after electronic cigarette fume exposure.
Figure 13 is the special animal used as test geometrical clamp holder structural representation of the present invention.
In figure：2-1 is front port, and 2-2 is that encephalic micro-dialysis sample skylight, and 2-3 is head lock chamber, and 2-4 is fixed for body portion
Chamber, 2-5 is push rod, and 2-6 is end cap, and 2-7 is hold-doun nut, and 2-8 is that arc pushes head.
Specific embodiment
The present invention is described further with the following Examples, but is not intended to limit the present invention.
1. experiment material：Male adult SD normal rat, 10 week old, body weight（200 ± 20）G, is raised using independent isolating
Foster cage tool is raised, and is freely intake, standard particle feed.
2. major experimental equipment：Animal mouth and nose suck fume exposure equipment, occur comprising rotating disc type smoking machine, aerosol
Device and animal mouth and nose suction exposing device（HRH-MNE63150, the intelligent Rong He companies in Beijing）；Microdialysis sgstem is (including CMA/402
Microdialysis pump, the on-line automatic injectors of CMA on-line injector, the microdialysis probes of CMA 12 and conduit, Sweden CMA is public
Department)；SN-1N type stereotaxic apparatus (Japanese Narishige companies)；High performance liquid chromatography-coulomb array electrochemical detector
（5600A CoulArray, Thermo companies of the U.S.）；Improved animal used as test nose suction exposure holder structure referring to
Figure 13（And separately apply for a patent）.
3. experimental technique：The method of the present invention is comprised the following steps：
（1）Microdialysis probe conduit brain is implanted into：After rat weight, 1.25 g/kg dosage are pressed with 20% urethane ester solution
Intraperitoneal injection of anesthesia, is fixed on stereotaxic instrument, opens cranium and removes part endocranium, according to the elements of a fix (AP：+2.5 mm；ML：‒
1.2 mm；DV：6.6 mm) it is implanted into rat brain nucleus accumbens septi and visits micro dialysis needle sleeve pipe and fixed with dental cement.Post operation,
Rat single cage is fed and is recovered 24 hours.
（2）The suction exposure of flue gas mouth and nose：Using rotating disc type smoking machine under the puffing regimenses of ISO 3308 (each pumping volume
For 35ml, 2s being aspirated, is spaced 60s) smoking cigarette produces cigarette smoke or tobacco juice for electronic smoke is added in aerosol generator
Produce electronic cigarette flue gas（Aerosol）, the experimental rat that brain is implanted with microdialysis probe conduit is placed in into improved animal used as test
In nose suction exposure clamper, in subsequently accessing animal mouth and nose suction exposing device；The suction exposure of precise control animal mouth and nose
The parameters such as temperature, the diluent air flow velocity of device, make experimental rat be exposed to constant TPM（TPM）In the flue gas of concentration.
（3）Microdialysis sampling：By microdialysis probe（CMA 12）In the probe catheter of loading test rat head and connect
Microdialysis pipeline, using the ringer's solution of filtration（Compound sodium chloride injection）Sampled with the speed perfusion of 2.0 μ L/min.
（4）Sample detection：By microdialysis sampling pipeline by on-line automatic injector and high performance liquid chromatography-coulomb array
Electrochemical detector connects the on-line automatic sample introduction to realize microdialysis sample and analyzes, according to the chromatogram row of 5 kinds of target compounds
For（Fig. 2）And analysis time（The detection of a sample can be completed in 14 min under following chromatographic conditions）Setting it is on-line automatic enter
The sampling interval of sample device is 15 min.
Chromatographic condition
Chromatographic column：Discovery HS F5 posts (4.6 mm × 150 mm, 3 μm)；Column temperature：30 ℃；Mobile phase：A：Methyl alcohol,
B：Buffering saline solution, containing 40 mmol/L citric acids, 20 mmol/L disodium hydrogen phosphates and 0.3 mmol/L EDTA；Flow velocity：0.25
mL/min；Condition of gradient elution is shown in Table 1；Sample size：20 µL；Coulomb array electrification is arranged according to the volt-ampere curve of each object
Learn detector 8 road work potentials be：‒50、0、50、175、200、350、400、500 mV.
The condition of gradient elution of table 1
| Time（min） | Mobile phase A（%） | Mobile phase B（%） |
| 0 | 10 | 90 |
| 4 | 25 | 75 |
| 13 | 25 | 75 |
| 14 | 10 | 90 |
（5）The foundation of calibration curve and sample data are analyzed
Serial mixed standard solution is carried out into HPLC-ECD point by concentration order from low to high in the case where Microdialysis sgstem is not connected
Analysis, with the chromatographic peak area of each object（Y）To its corresponding concentration（X）Linear regression is carried out, calibration curve recurrence side is obtained
Journey.
Quantitative analysis is carried out using 5 kinds of objects in the micro- sample of rat brain of the calibration curve method to detection, according to result
The Concentration-time change curve of the forward and backward each time point intracerebral monoamine neurotransmitter of fume exposure and its metabolin is drawn respectively,
Disclose affecting laws of the fume exposure to monoamine neurotransmitter.
Embodiment 1
Rat intracerebral monoamine neurotransmitter change detection test after certain reference cigarette smoke exposure
According to above-mentioned steps（1）Rat brain microdialysis probe conduit Implantation Test is carried out, subsequently according to step（2）Carry out reference
Cigarette smoking and the suction exposure of rat flue gas mouth and nose formula, make experimental rat be exposed to TPM concentration for 200 mg/m3Cigarette smoke
In, then according to step（3）Microdialysis experiments are carried out, according to step（4）Sample detection is carried out, finally according to step（5）Carry out
The data analysis of sample.
Fig. 3, Fig. 4, Fig. 5, Fig. 6 and Fig. 7 be respectively cigarette smoke exposure before and after rat intracerebral DA, DOPAC, HVA, 5-HT and
The Concentration-time change curve schematic diagram of 5-HIAA.
As a result show, during cigarette smoke exposes, the release of DA and 5-HT in rat nucleus accumbens septi continues to increase, the primary of DA
The content of metabolite DOPAC is without significant changes, and the content of DA and 5-HT respective whole level metabolite HVA and 5-HIAA is bright
It is aobvious to raise, illustrate that the inventive method can be used for the change of rat intracerebral monoamine neurotransmitter before and after the exposure of continuous monitoring cigarette smoke
Law.
Embodiment 2
Rat intracerebral monoamine neurotransmitter change detection test after certain electronic cigarette fume exposure
According to above-mentioned steps（1）Rat brain microdialysis probe conduit Implantation Test is carried out, subsequently according to step（2）It is molten using gas
Glue generator carries out the generation of electronics smoke sol and the suction exposure of rat flue gas mouth and nose formula, makes experimental rat be exposed to TPM dense
Spend for 1000 mg/m3Electronic cigarette flue gas in, then according to step（3）Microdialysis experiments are carried out, according to step（4）Carry out sample
Product examine is surveyed, finally according to step（5）Carry out the data analysis of sample.
Fig. 8, Fig. 9, Figure 10, Figure 11 and Figure 12 be respectively rat intracerebral DA, DOPAC before and after electronic cigarette fume exposure, HVA,
The Concentration-time change curve schematic diagram of 5-HT and 5-HIAA.As a result show, between electronic cigarette flue gas exposure period in rat nucleus accumbens septi
The change of DA and 5-HT presents and rule as cigarette smoke exposure phase, illustrates that the inventive method can also be used for real-time monitoring
The change information of rat intracerebral monoamine neurotransmitter before and after electronic cigarette fume exposure.
Claims (4)
1. a kind of method that animal intracerebral monoamine neurotransmitter changes under the conditions of real-time detection fume exposure, it is characterised in that：
Animal is exposed in real time in flue gas using animal mouth and nose formula suction fume exposure system, collects dynamic by on-line microdialysis technology
Thing brain tissue sample, recycles high performance liquid chromatography-coulomb array electrochemical detector to the monoamine in brain dialysate samples
Neurotransmitter and its metabolin are quickly analyzed, so as to animal intracerebral monoamine neurotransmitter before and after real-time monitoring fume exposure
Variation characteristic, comprise the following steps that：
A. fume exposure：Electronic cigarette liquid is set to produce aerosol using smoking machine smoking cigarette or using aerosol generator, by head
Animal equipped with microdialysis probe conduit is put into a special animal used as test geometrical clamp holder in portion, so that the clamper seizes dynamic on both sides by the arms
Thing is only exposed in certain density flue gas with mouth and nose suction；
B. encephalic micro-dialysis sample collection：Microdialysis probe is inserted continuous in the conduit of animal head after operation Microdialysis sgstem
Animal brain dislysate is collected in the quantitative loop of online injector, at regular intervals operation is automatically injected by online injector
High performance liquid chromatography in；
C. high performance liquid chromatography-coulomb array electrochemical detector analysis：Set gradient elution program and sample introduction sequence and run
High performance liquid chromatography-coulomb array electrochemical detector detection microdialysis sample so that the detection time of each sample is slightly less than
The acquisition time of the sample；
D. standard liquid is prepared and Specification Curve of Increasing：Prepare containing dopamine (DA), 3,4 dihydroxyphenyl acetic acids (DOPAC),
Homovanillic acid (HVA), 5 kinds of standard items of serotonin (5-HT) and 5 hydroxyindole acetic acids (5-HIAA) serial hybrid standard it is molten
Liquid, high performance liquid chromatography-coulomb array electrochemical detection is carried out by serial mixed standard solution by concentration order from low to high
Device is analyzed, with the chromatographic peak area of each target analytes（Y）To its corresponding concentration（X）Linear regression is carried out, standard is obtained bent
Line regression equation；
E. data analysis and process：The content of each object in dialysate samples is calculated according to calibration curve method, according to result point
Not Hui Zhi the forward and backward each time point intracerebral monoamine neurotransmitter of fume exposure and its metabolin Concentration-time change curve, take off
Show the rule that fume exposure causes intracerebral monoamine neurotransmitter to change.
2. the side that animal intracerebral monoamine neurotransmitter changes under the conditions of real-time detection fume exposure according to claim 1
Method, it is characterised in that：Standard liquid process for preparation is specific as follows：5 kinds of DA, DOPAC, HVA, 5-HT and 5-HIAA is weighed respectively
The each 10mg of standard items, in being respectively placed in 100mL volumetric flasks, uses methanol/water solution（20:80, volume ratio, similarly hereinafter）Dissolve and dilute
To scale, shake up, it is respectively the Standard Stock solutions of 100 μ g/mL to obtain concentration；The Standard Stock solutions of 1.0mL are pipetted respectively, are placed in
In same 100mL volumetric flask, scale is diluted to methanol/water, is shaken up, it is respectively the one-level mixing mark of 1.0 μ g/mL to obtain concentration
Quasi- solution；0.01,0.05,0.10,0.50,1.00,5.00,10.0 and 50.0mL one-level mixed standard solutions are accurately pipetted, point
In not being placed in different 100mL volumetric flasks, with compound sodium chloride injection scale is diluted to, is shaken up, obtain concentration it is equal 0.1,
0.5th, 1.0,5.0,10.0,50.0,100.0 and 500.0ng/mL serial mixed standard solution.
3. the side that animal intracerebral monoamine neurotransmitter changes under the conditions of real-time detection fume exposure according to claim 1
Method, it is characterised in that：Chromatographic condition in step c is specific as follows：
Chromatographic column：Discovery HS F5 posts, mm × 150 mm of specification 4.6,3 μm；Column temperature：30 ℃；Mobile phase：A：First
Alcohol, B：Buffering saline solution, containing 40 mmol/L citric acids, 20 mmol/L disodium hydrogen phosphates and 0.3 mmol/L EDTA；Flow velocity：
0.25 mL/min；Condition of gradient elution such as following table：
。
4. the side that animal intracerebral monoamine neurotransmitter changes under the conditions of real-time detection fume exposure according to claim 1
Method, it is characterised in that：The animal is mouse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611241605.5A CN106645492B (en) | 2016-12-29 | 2016-12-29 | Under the conditions of a kind of real-time detection fume exposure in animal brain monoamine neurotransmitter variation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611241605.5A CN106645492B (en) | 2016-12-29 | 2016-12-29 | Under the conditions of a kind of real-time detection fume exposure in animal brain monoamine neurotransmitter variation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106645492A true CN106645492A (en) | 2017-05-10 |
| CN106645492B CN106645492B (en) | 2019-06-07 |
Family
ID=58835416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611241605.5A Active CN106645492B (en) | 2016-12-29 | 2016-12-29 | Under the conditions of a kind of real-time detection fume exposure in animal brain monoamine neurotransmitter variation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645492B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459095A (en) * | 2018-01-17 | 2018-08-28 | 烟台大学 | Detection method that is a kind of while detecting eight kinds of neurotransmitters |
| CN108802250A (en) * | 2018-08-28 | 2018-11-13 | 上海中医药大学 | The method that ultra high efficiency LC-MS detects 11 kinds of neurotransmitters in encephalic micro-dialysis liquid |
| CN112730738A (en) * | 2020-12-07 | 2021-04-30 | 中国科学院深圳先进技术研究院 | Evaluation device and evaluation method for reward and addiction of atomized matter |
| CN114609300A (en) * | 2022-04-07 | 2022-06-10 | 国家烟草质量监督检验中心 | Method for evaluating influence of menthol addition on nicotine metabolism of oral nicotine based on animal model |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102455313A (en) * | 2010-11-01 | 2012-05-16 | 上海纳米技术及应用国家工程研究中心有限公司 | Lamina detection pool used for detecting dopamine and preparation method thereof |
| CN104020241A (en) * | 2014-06-11 | 2014-09-03 | 中国烟草总公司郑州烟草研究院 | Synchronous analysis method for trace nicotine in blood-brain samples of animal and main metabolites thereof |
| RU2013113102A (en) * | 2013-03-25 | 2014-09-27 | Татьяна Михайловна Рожнова | METHOD FOR FORECASTING RISK OF DEVELOPMENT OF DISORDERS OF BEHAVIOR OF A DEPENDENT CHARACTER IN THE FORM OF ADDICTION, ALCOHOLISM, TOBACCO SMOKING AND / OR THE ACCEPTANCE PHENOMENON |
| CN105891357A (en) * | 2016-04-05 | 2016-08-24 | 中国烟草总公司郑州烟草研究院 | Analysis method for dynamically tracing and monitoring nicotine and nicotine metabolites in animal |
-
2016
- 2016-12-29 CN CN201611241605.5A patent/CN106645492B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102455313A (en) * | 2010-11-01 | 2012-05-16 | 上海纳米技术及应用国家工程研究中心有限公司 | Lamina detection pool used for detecting dopamine and preparation method thereof |
| RU2013113102A (en) * | 2013-03-25 | 2014-09-27 | Татьяна Михайловна Рожнова | METHOD FOR FORECASTING RISK OF DEVELOPMENT OF DISORDERS OF BEHAVIOR OF A DEPENDENT CHARACTER IN THE FORM OF ADDICTION, ALCOHOLISM, TOBACCO SMOKING AND / OR THE ACCEPTANCE PHENOMENON |
| CN104020241A (en) * | 2014-06-11 | 2014-09-03 | 中国烟草总公司郑州烟草研究院 | Synchronous analysis method for trace nicotine in blood-brain samples of animal and main metabolites thereof |
| CN105891357A (en) * | 2016-04-05 | 2016-08-24 | 中国烟草总公司郑州烟草研究院 | Analysis method for dynamically tracing and monitoring nicotine and nicotine metabolites in animal |
Non-Patent Citations (3)
| Title |
|---|
| ANNA CZUBAK等: "Effect of venlafaxine and nicotine on the level of neurotransmitters and their metabolites in rat brains", 《JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY》 * |
| 肖卫强等: "不同模式口鼻式吸烟诱导急性肺损伤的实验研究", 《浙江大学学报（医学版）》 * |
| 邓少东等: "库仑阵列电化学-高效液相色谱法同时检测大鼠不同脑区中的8种单胺类神经递质", 《广东医学院学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459095A (en) * | 2018-01-17 | 2018-08-28 | 烟台大学 | Detection method that is a kind of while detecting eight kinds of neurotransmitters |
| CN108802250A (en) * | 2018-08-28 | 2018-11-13 | 上海中医药大学 | The method that ultra high efficiency LC-MS detects 11 kinds of neurotransmitters in encephalic micro-dialysis liquid |
| CN112730738A (en) * | 2020-12-07 | 2021-04-30 | 中国科学院深圳先进技术研究院 | Evaluation device and evaluation method for reward and addiction of atomized matter |
| CN114609300A (en) * | 2022-04-07 | 2022-06-10 | 国家烟草质量监督检验中心 | Method for evaluating influence of menthol addition on nicotine metabolism of oral nicotine based on animal model |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106645492B (en) | 2019-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106645492A (en) | Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition | |
| Kennedy | Emerging trends in in vivo neurochemical monitoring by microdialysis | |
| Robinson et al. | Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo | |
| Robinson et al. | Microdialysis in the neurosciences: techniques in the behavioral and neural sciences | |
| Kennedy et al. | In vivo monitoring of amino acids by direct sampling of brain extracellular fluid at ultralow flow rates and capillary electrophoresis | |
| Poirier et al. | Carcinogen macromolecular adducts and their measurement | |
| CN102012409B (en) | Analysis method for trace tobacco specific N-nitrosamine (TSNAs) in animal blood sample | |
| CN105891357B (en) | The analysis method of nicotine and its metabolin in a kind of dynamic tracer monitoring animal body | |
| Tao et al. | Alteration in regulation of serotonin release in rat dorsal raphe nucleus after prolonged exposure to morphine | |
| US11275077B2 (en) | Olfactory detector for early diagnosis of ovarian cancer | |
| CN103869041B (en) | A kind of detection method of cough-relieving combination of oral medication | |
| CN104020241A (en) | Synchronous analysis method for trace nicotine in blood-brain samples of animal and main metabolites thereof | |
| CN108181387A (en) | The new method of phospholipid fraction content detection in a kind of Curosurf bulk pharmaceutical chemicals | |
| CN104198619A (en) | Quality detection method of cough syrup for children | |
| Andrews et al. | Extensive connections of the canine olfactory pathway revealed by tractography and dissection | |
| WO2009029936A1 (en) | In vivo imaging of myelin | |
| CN1785281B (en) | Quality control method of spirit quieting oral liquid preparation | |
| CN102078403A (en) | Detection method of Chinese traditional medicine preparation of oral liquid for treating infant cough with lung heat | |
| US20130131470A1 (en) | Methods and systems for in vivo clinical data measurement of analytes | |
| Steiner et al. | Delivery efficiencies of constituents of combustion-derived aerosols across the air-liquid interface during in vitro exposures | |
| Li et al. | Direct Determination of Free Nicotine Content in Tobacco | |
| MEssERLI et al. | Electrochemical sensor applications to the study of molecular physiology and analyte flux in plants | |
| CN106525994A (en) | Method for determination of related substances of paracetamol and tramadol hydrochloride capsules | |
| Ahemaiti et al. | A multifunctional pipette for localized drug administration to brain slices | |
| Morales-Villagrán et al. | A capillary fraction collector coupled to a fluorescence reader: a novel device to continuously quantify glutamate during microdialysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |