CN106645469A - Method for detecting aldehyde ketone compound - Google Patents
Method for detecting aldehyde ketone compound Download PDFInfo
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- CN106645469A CN106645469A CN201611110444.6A CN201611110444A CN106645469A CN 106645469 A CN106645469 A CN 106645469A CN 201611110444 A CN201611110444 A CN 201611110444A CN 106645469 A CN106645469 A CN 106645469A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention discloses a method for detecting an aldehyde ketone compound. The method comprises the following steps: enabling the aldehyde ketone compound to react with dansyl hydrazine under an acid condition to obtain a hydrazone compound; and separating the hydrazone compound by virtue of a high-performance liquid chromatography, and performing qualitative and quantitative analysis by adopting a fluorescent detector. The method is simple, high in sensitivity, low in detection limit, and capable of being well applied to the actual sample analysis, and providing evidence for the low-content aldehyde ketone detection method.
Description
Technical field
The present invention relates to a kind of method of detection group compounds of aldehydes and ketones, more particularly to one kind is using red sulfohydrazide as aldoketones
Compound conversion reagent, by the method for fluoroscopic examination group compounds of aldehydes and ketones, belongs to technical field of analysis and detection.
Background technology
Aldoketones material is common conventional chemical reagent, including formaldehyde, acetaldehyde, propionic aldehyde, butyraldehyde, acetone etc., these
Belong to small molecule, low boiling aldehyde ketone, being easily released in air or be transferred on contactant directly or indirectly causes various diseases
Disease.Wherein, formaldehyde can cause to damage to nasal cavity, lung, the hemopoietic system of human body, and improve the risk that cancer is suffered from each position, state
Formaldehyde is attributed to suspect carcinogen class by border Agency for Research on Cancer (IARC).Other aldehyde ketone Long Term Contacts, can be to a certain degree
Upper infringement health, or even cause a series of complication.
At present, the detection method of aldehyde ketone conventional both at home and abroad is DNPH (DNPH) column front derivation-HPLC/
PDA detected, the following reaction equation 1 of its derivative principle, and related conventional detection method is also based on this principle in industry
Carry out.But based on the restriction of PDA detector sensitivities, its test limit is in the level of a high value, for sample
The detection of the aldehyde ketone of low concentration in product is unable to reach with monitoring.
Wherein, R1And R2It is independent to select hydrogen, Methylethyl etc.；
Reaction equation 1：The derivative aldehyde ketone principles of DNPH
And fluorescence detector (FLD) sensitivity is higher than PDA detector, but selection of the fluorescence detector to derivatization reagent
There is higher requirement, needing its own to send fluorescence or product can send fluorescence, therefore, find suitable derivatization reagent
It is the key for realizing fluoroscopic examination group compounds of aldehydes and ketones.
The content of the invention
The defect that the method for group compounds of aldehydes and ketones is present is detected for existing PDA, it is to provide that the purpose of the present invention is
A kind of method is simple, and sensitivity is high, the analyzing detecting method of the low low content aldehyde ketone of detection limit, and the method is based on fluoroscopic examination aldehyde
Ketone compounds, can well be applied to the detection of sample in real life, be conducive to popularization and application.
In order to realize above-mentioned technical purpose, the invention provides a kind of method of detection group compounds of aldehydes and ketones, in acid bar
Under part, by group compounds of aldehydes and ketones and red sulphonyl hydrazine reaction, hydrazone compounds are obtained；The hydrazone compounds pass through high-efficient liquid phase color
After spectrum is separated, qualitative and quantitative analysis are carried out using fluorescence detector.
Technical scheme makes aldehyde first from red sulfohydrazide (DNSH) as the conversion reagent of group compounds of aldehydes and ketones
Ketone compounds are changed into and can be used for high performance liquid chromatography mark, and the hydrazone compounds that can be detected by fluorescence detector, with reality
Existing high performance liquid chromatography separation and fluoroscopic examination (FLD), to reach high sensitivity and low detection limits group compounds of aldehydes and ketones is detected
Purpose.
Technical solution of the present invention, under acid medium existence condition, DNSH is easy to be reacted life with group compounds of aldehydes and ketones
Into metastable hydrazone compounds, because its structure itself is comprising having big conjugated structure, can be used for high performance liquid chromatography
Mark, and can be detected by fluorescence detector.The reaction mechanism such as reaction equation 2 of DNSH and group compounds of aldehydes and ketones.The present invention's
The method that technical scheme exactly establishes detection group compounds of aldehydes and ketones using the principle, the method has simple, sensitivity height, inspection
The low feature of rising limit, the detection for low content aldehyde ketone provides method foundation.
Reaction equation 2：The derivative aldehyde ketone principles of DNSH
Preferred scheme, group compounds of aldehydes and ketones reacts with red sulfohydrazide in glacial acetic acid/acetonitrile mixed solution, obtains hydrazone class
Compound.DNSH is soluble in polar solvent such as ethanol, acetonitrile etc., but decomposes easily in water, and stability is poor, the technology of the present invention
Scheme is reacted in nonaqueous solvents.Meanwhile, active stronger lone pair electrons are contained on red sulfohydrazide ammonia nitrogen, in acid condition
Under, there is nucleophilic addition in energy attack carbonyl carbon.
More preferably scheme, reaction temperature is 5～45 DEG C, and the reaction time is 12～30h.
More preferably scheme, group compounds of aldehydes and ketones includes at least one of formaldehyde, acetaldehyde, propionic aldehyde, butyraldehyde, acetone.
More preferably scheme, the liquid chromatogram column type number that the high performance liquid chromatography is adopted：C18 (2),
4.6mm × 250mm, 5 μm；
Mobile phase A：Water, Mobile phase B：Acetonitrile；
Elution program：Gradient elution, 0min, 55vol%A+45vol%B；12min, 55vol%A+45vol%B；
15min, 40vol%A+60vol%B；20min, 10vol%A+90vol%B；21min, 55vol%A+45vol%B,
25min, 55vol%A+45vol%B；
Flow velocity：1.0mL·min-1；
Sample size：10μL；
Column temperature：30℃.
More preferably scheme, fluorescence detector is 2475 fluorescence detectors, Detection wavelength λ ex=330nm, λ em=
484nm。
In technical scheme, red sulfohydrazide adopts acetic acid/acetonitrile solution to be configured to concentration for 500mgL-1Pellet
Sulphonyl hydrazine solution, concrete compound method is：In accurately weighing red sulfohydrazide (DNSH) 25mg to 50mL brown volumetric flasks, add suitable
3% (percentage by volume) glacial acetic acid/acetonitrile mixed solution of amount, it is molten with glacial acetic acid/acetonitrile mixing after vibration is completely dissolved DNSH
Liquid is settled to scale.
The group compounds of aldehydes and ketones that technical scheme can be used in detection sample, such as paper, the aldehyde in sample
Ketone compounds are first extracted using acetonitrile, then change into group compounds of aldehydes and ketones using red sulphonyl hydrazine solution can to carry out fluorescence inspection
The hydrazone compounds of survey.Content of group compounds of aldehydes and ketones is specifically described in detect paper：Weigh testing sample (such as paper)
1.5g adds acetonitrile 15mL, oscillation extraction 30min in 50mL conical flask with cover, toward conical flask, and about 8mL upper stratas are taken after standing
Clear liquid is centrifuged 10min to 10mL centrifuge tubes under 12000r/min rotating speeds, takes the supernatant liquor 5mL after centrifugation red to 2mL is equipped with
In the 10mL brown volumetric flasks of sulphonyl hydrazine solution and 90 μ L glacial acetic acid, and scale is settled to acetonitrile, reacts 24h, cross 0.45 μm
After organic phase filter membrane, using efficient liquid phase chromatographic analysis and fluoroscopic examination.
The compound method of the fluorescence detection Plays working solution of the present invention：
1) configuration of hybrid standard storing solution
In accurately pipetting sulphonyl hydrazine solution 5mL to 10mL brown volumetric flasks, then each group compounds of aldehydes and ketones is accurately pipetted respectively
The each 20 μ L of standard items after vibration is mixed, with acetonitrile solution scale are settled to in above-mentioned volumetric flask, are configured to each aldoketones chemical combination
The hydrazone compounds concentration of thing is 2mgL-1One-level hybrid standard storing solution.Accurately pipette 5.00mL one-level hybrid standards
Storing solution is settled to scale in 10mL brown volumetric flasks with 1.5vol.% glacial acetic acid/acetonitrile solution, used as two grades of mixing marks
Quasi- storing solution.
2) configuration of hybrid standard working solution
Accurately pipette 0.100 respectively, 0.200,0.500,1.000,2.000, bis- grades of hybrid standard storing solutions of 4.000mL arrive
In 10mL brown volumetric flasks, with 1.5vol.% glacial acetic acid/acetonitrile solution scale is settled to, obtain concentration be respectively 10.000,
20.000、50.000、100.000、200.000、400.000μg·L-1Hybrid standard working solution.
Hinge structure, the Advantageous Effects that technical scheme is brought：Technical scheme is first
From red sulfohydrazide (DNSH) as the conversion reagent of group compounds of aldehydes and ketones, change into group compounds of aldehydes and ketones and can be used for efficient liquid
Phase chromatogram is marked, and the hydrazone compounds that can be detected by fluorescence detector, realizes the fluoroscopic examination of group compounds of aldehydes and ketones
(FLD), with simple, sensitivity is high, the low feature of detection limit, for low content group compounds of aldehydes and ketones detection provide method according to
According to.
Description of the drawings
【Fig. 1】Process chart.
【Fig. 2】For the chromatogram of the group compounds of aldehydes and ketones of 5 kinds of selections in standard liquid and certain outturn；
Wherein, 1 is formaldehyde-pellet sulphonyl hydrazone, and 2 is acetaldehyde-pellet sulphonyl hydrazone；3 is acetone-pellet sulphonyl hydrazone；4 is propionic aldehyde-pellet sulphur
Acylhydrazone；5 is butyraldehyde-pellet sulphonyl hydrazone.
Specific embodiment
It is right below by taking the assay method that the derivative-HPLC/FLD methods of DNSH determine five kinds of aldehyde ketones in certain paper simultaneously as an example
The present invention is further detailed, without limiting the claims in the present invention protection domain.
Embodiment 1
1st, instrument, reagent and material
Instrument：Waters e2695 high performance liquid chromatographs (Waters, US), is equipped with automatic sampler, quaternary
Pump, column oven and 2475 fluorescence detectors；BSA224S-CW assay balances (German Sartorius electronic balances company)；HY-6
Double-deck klystron oscillator instrument (Guo Hua Electrical Appliances Co., Ltd)；(Kunshan ultrasonic instrument is limited for KQ-600DE types numerical control supersonic cleaning device
Company)；TGL-16M desk centrifuges (Xiang Yi centrifuges Instrument Ltd.)；The Milli-Q50 ultra-pure water instrument (U.S.
Millipore companies)；1uL-5mL piston type liquid-transfering guns (German Eppendorf companies)；C18(2)(4.6mm
× 250mm, 5 μm) liquid-phase chromatographic column.
Reagent：
The standard items of table 1 and reagent
Water used is ultra-pure water.
2nd, the preparation of solution
DNSH solution：In accurately weighing red sulfohydrazide (DNSH) 25mg to 50mL brown volumetric flasks, appropriate 3% (body is added
Product percentage) glacial acetic acid/acetonitrile mixed solution, after vibration is completely dissolved DNSH, with the solution scale is settled to.DNSH solution
Middle DNSH concentration is 500mgL-1。
The preparation of hybrid standard storing solution：In accurately pipetting derivatization reagent 5mL to 10mL brown volumetric flasks, then standard respectively
The each 20 μ L of formaldehyde, acetaldehyde, acetone, propionic aldehyde, butyraldehyde standard items are really pipetted in above-mentioned volumetric flask, is vibrated after mixing, it is molten with acetonitrile
Liquid is settled to scale, is configured to formaldehyde-hydrazone, acetaldehyde-hydrazone, propionic aldehyde-hydrazone, butyraldehyde-hydrazone, acetone-hydrazone concentration and is 2mg
L-1One-level hybrid standard storing solution.5.00mL one-level hybrid standard storing solutions are accurately pipetted in 10mL brown volumetric flasks, is used
1.5% glacial acetic acid/acetonitrile mixed solution is settled to scale, used as two grades of hybrid standard storing solutions.
The preparation of hybrid standard working solution：Accurately pipette 0.100 respectively, 0.200,0.500,1.000,2.000,
Bis- grades of hybrid standard storing solutions of 4.000mL are settled to in 10mL brown volumetric flasks with 1.5% glacial acetic acid/acetonitrile mixed solution
Scale, obtains concentration and is respectively 10.000,20.000,50.000,100.000,200.000,400.000 μ gL-1 mixing marks
Quasi- working solution.Keep in dark place in 4 DEG C of refrigerators, the term of validity 3 months.
3rd, sample pre-treatments
Weigh by regulation cutting paper chips 1.5g in 50mL conical flask with cover, acetonitrile 15mL added toward conical flask,
Oscillation extraction 30min, takes about 8mL supernatant liquors to 10mL centrifuge tubes after standing, 10min is centrifuged under 12000r/min rotating speeds,
The supernatant liquor 5mL after centrifugation is taken in the 10mL brown volumetric flasks equipped with 2mL derivatization reagents and 90 μ L glacial acetic acid, and uses second
Nitrile is settled to scale, and derivative reaction 24h then crosses 0.45 μm of organic phase filter membrane and treats efficient liquid phase chromatographic analysis.
4th, detect
Instrument condition
Liquid-phase chromatographic column：C18 (2) (4.6mm × 250mm, 5 μm)；Detector：2475 fluorescence detectors, λ
Ex=330nm, λ em=484nm；Mobile phase A：Water, Mobile phase B：Acetonitrile；Elution program：Gradient elution, 0min, (55%A+
45%B), 12min, (55%A+45%B), 15min, (40%A+60%B), 20min (10%A+90%B), 21min, (55%
A+45%B), 25min, (55%A+45%B)；Flow velocity：1.0mL·min-1；Sample size：10μL；Column temperature：30℃.Using reservation
Time is qualitative, quantified by external standard method.
Analysis detecting step：
Series standard working solution is measured under chromatographic condition, it is dense to its quality according to the peak area (μ V*S) for measuring
Degree ρ (μ gL-1) carries out linear regression, with peak area (μ V*S) as Y-axis, maps by X-axis of mass concentration ρ (μ gL-1), obtains
To equation of linear regression.The range of linearity of each aldehyde ketone, equation of linear regression, coefficient correlation, detection limit and quantitative limit are shown in Table 2, side
The recovery of standard addition and precision of method is shown in Table 3.
The range of linearity of table 2, equation of linear regression, coefficient correlation, detection limit and quantitative limit
The method rate of recovery of table 3 and precision result (n=5)
The detection of outturn：Sample is detected under the same conditions, by the 5 kinds of Carbonyl compounds to be measured for obtaining
Chromatographic peak area is brought in corresponding calibration curve regression equation, you can obtain containing for corresponding 5 kinds of Carbonyl compounds in sample
Amount.
5th, cubage
The measurement result of 5 kinds of Carbonyl compounds, calculates according to the following formula in certain paper：
Q=c × V/m
In formula：
Q is 5 kinds of Carbonyl compounds contents in paper, and unit is μ g/kg；
C is the concentration of 5 kinds of Carbonyl compounds in paper, and unit is μ g/L；
V is extract volume (mL)；
M be claim take outturn weight, unit is g.
The measure of 5 kinds of Carbonyl compounds contents adopts the inventive method in test specimen, and measurement result is as shown in table 4：
The outturn analysis result of table 4
Note："-" is represented less than quantitative limit, not detected.
Claims (6)
1. it is a kind of detection group compounds of aldehydes and ketones method, it is characterised in that：In acid condition, group compounds of aldehydes and ketones and red sulphonyl
Hydrazine reaction, obtains hydrazone compounds；The hydrazone compounds are carried out after high performance liquid chromatography separation using fluorescence detector
Qualitative and quantitative analysis.
2. it is according to claim 1 detection group compounds of aldehydes and ketones method, it is characterised in that：Group compounds of aldehydes and ketones and red sulphur
Hydrazides reacts in glacial acetic acid/acetonitrile mixed solution, obtains hydrazone compounds.
3. it is according to claim 2 detection group compounds of aldehydes and ketones method, it is characterised in that：Reaction temperature is 5～45 DEG C,
Reaction time is 12～30h.
4. it is according to claim 2 detection group compounds of aldehydes and ketones method, it is characterised in that：Described group compounds of aldehydes and ketones
Including at least one of formaldehyde, acetaldehyde, propionic aldehyde, butyraldehyde, acetone.
5. according to any one of Claims 1 to 4 detection group compounds of aldehydes and ketones method, it is characterised in that：
The liquid chromatogram column type number that the high performance liquid chromatography is adopted：C18 (2), 4.6mm × 250mm, 5 μm；Stream
Dynamic phase A：Water, Mobile phase B：Acetonitrile；
Elution program：Gradient elution,
0min, 55vol%A+45vol%B；
12min, 55vol%A+45vol%B；
15min, 40vol%A+60vol%B；
20min, 10vol%A+90vol%B；
21min, 55vol%A+45vol%B；
25min, 55vol%A+45vol%B；
Flow velocity：1.0mL·min-1；
Sample size：10μL；
Column temperature：30℃.
6. according to any one of Claims 1 to 4 detection group compounds of aldehydes and ketones method, it is characterised in that：Described is glimmering
Photodetector be 2475 fluorescence detectors, Detection wavelength λ ex=330nm, λ em=484nm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333277A (en) * | 2018-05-11 | 2018-07-27 | 东莞市中鼎检测技术有限公司 | The detection method of acetaldehyde in a kind of dyestuff |
| CN109239039A (en) * | 2018-09-30 | 2019-01-18 | 河南省农业科学院农业质量标准与检测技术研究所 | A kind of acetaldehyde detection method and its application based on fluorescence probe |
| CN112114081A (en) * | 2020-08-14 | 2020-12-22 | 广州中科检测技术服务有限公司 | Detection method for rapidly identifying quality of cold-pressed peanut oil |
| CN113138246A (en) * | 2021-04-25 | 2021-07-20 | 深圳市中医院 | Detection method for targeted determination of short-chain fatty acid in biological sample |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103128A (en) * | 2011-03-24 | 2011-06-22 | 中国烟草总公司郑州烟草研究院 | Method for determining contents of formaldehyde, acetaldehyde and acetone in water-borne adhesives for cigarettes |
-
2016
- 2016-12-06 CN CN201611110444.6A patent/CN106645469A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103128A (en) * | 2011-03-24 | 2011-06-22 | 中国烟草总公司郑州烟草研究院 | Method for determining contents of formaldehyde, acetaldehyde and acetone in water-borne adhesives for cigarettes |
Non-Patent Citations (2)
| Title |
|---|
| J.ZHANG 等: "Development of the Personal Aldehydes and Ketones Sampler Based upon DNSH Derivatization on Solid Sorbent", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
| 余晶晶 等: "LC-ESI-MS/MS 法检测卷烟滤嘴中8 种挥发性羰基化合物", 《烟草科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333277A (en) * | 2018-05-11 | 2018-07-27 | 东莞市中鼎检测技术有限公司 | The detection method of acetaldehyde in a kind of dyestuff |
| CN109239039A (en) * | 2018-09-30 | 2019-01-18 | 河南省农业科学院农业质量标准与检测技术研究所 | A kind of acetaldehyde detection method and its application based on fluorescence probe |
| CN109239039B (en) * | 2018-09-30 | 2020-10-27 | 河南省农业科学院农业质量标准与检测技术研究所 | Acetaldehyde detection method based on fluorescent probe and application thereof |
| CN112114081A (en) * | 2020-08-14 | 2020-12-22 | 广州中科检测技术服务有限公司 | Detection method for rapidly identifying quality of cold-pressed peanut oil |
| CN113138246A (en) * | 2021-04-25 | 2021-07-20 | 深圳市中医院 | Detection method for targeted determination of short-chain fatty acid in biological sample |
| CN113138246B (en) * | 2021-04-25 | 2023-02-10 | 深圳市中医院 | Detection method for targeted determination of short-chain fatty acid in biological sample |
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Application publication date: 20170510 |