CN106645116A - Smartphone two-dimensional code based biological rapid detection method - Google Patents
Smartphone two-dimensional code based biological rapid detection method Download PDFInfo
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- CN106645116A CN106645116A CN201610863598.6A CN201610863598A CN106645116A CN 106645116 A CN106645116 A CN 106645116A CN 201610863598 A CN201610863598 A CN 201610863598A CN 106645116 A CN106645116 A CN 106645116A
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- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a smartphone two-dimensional code based biological rapid detection method. According to the rapid detection method, a detection reaction vessel is prepared, a reaction graph is formed by splicing modules, the modules are same in size and different in color and can be freely dismantled and assembled, a detection agent is added into the detection reaction vessel, a chromogenic reaction is conducted, a phone camera is used to scan the reaction result, the detection result is processed and displayed by a smartphone detection procedure after the reaction, and the rapid detection is conducted. The biological reaction is rapidly detected on the basis of a smartphone, and the smartphone two-dimensional code based biological rapid detection method has the advantages of high compatibility, convenience in operation, low cost and the like, and is suitable for rapid detection of various creatures on site.
Description
Technical field
The present invention relates to a kind of biological method for quick, has specifically write a kind of collection automatic using Java language
The software of one is encoded, decodes, being detected on, the detection reaction vessels printed using 3D printer, the molecule based on colorimetric method is known
Not, required according to detection, the two-dimensional bar generated needed for experiment is set, be capable of achieving any biological color development reaction, by reaction knot
The mobile phone A pp scanning for designing of result after beam, draws testing result, enters but realizes the colour developing of the quantitative determination to sample
Reaction.
Background technology
In the 21 century that economic globalization, information networking, production internationalization are with rapid changepl. never-ending changes and improvements, new and high technology is not only composition
The key element of overall national strength, and the every field in the national economic development has been permeated, as information technology important composition portion
Point two-dimensional bar code this new and high technology, be on the premise of bar code cannot meet modern information industry technology growth requirement
Produce.It solves the problems, such as to perplex being described " article " with bar code of people always, makes bar code veritably become letter
Breath storage and the effective tool for recognizing.Two-dimensional bar code has information capacity big relative to bar code, and reliability is high, can represent figure
The kinds of words information such as picture, Chinese character, the advantages of security and antiforge purpose is strong.Two-dimensional bar code be mainly characterized by two-dimensional bar code in level and
Vertical both direction represents data message.According to its generating principle and planform, the row's of going formula two-dimensional bar code can be classified as
And matrix two-dimensional barcode.Row row's formula two-dimensional bar code is on the basis of bar code, after two rows or multirow are highly truncated
Bar code accumulation, realize that information is represented on the basis of row identification, the characteristic such as error correcting is increased.Matrix type two-dimension
Bar code feature with matrix in planform.It based on computer image processing technology, in matrix respective element
On position, represented binary " 1 " with the appearance of square point, round dot etc., occurring without for point represents binary " 0 ", by point
Different permutation and combination represent data message.Jiang et al.(Y. Zhang, L. B. Qiao, Y. K. Ren, X. W.
Wang, M. Gao, Y. F. Tang, J. Z. J. Xi, T-Y Fu and X. Y. Jiang,
Biomicrofludics, 2013, 7, 034110, Two dimensional barcode-inspired automatic
analysis for arrayed microfluidic immunoassays)By Data Matrix and QR in two-dimensional bar code
Code has exciting for automatic positioning function, devises the microchannel of two-dimensional structure, and the design of the microchannel is by asymmetric
Red stripes are surrounded to determine detection zone, then reacted result are scanned with hand-held scanning means, last profit
Result is analyzed with computer software, realizes that high flux is detected, reach the purpose of quantitative determination.The quick letter of the method operation
Just, just with the concept of Quick Response Code automatic positioning function, coding rule is not incorporated, is not two dimension truly
Code.Meanwhile, the seminar(Y. Zhang, J. Sun, Y. Zou, W. W. Chen, W. Zhang, J. Z. J. Xi,
and X. Y. Jiang, Anal. Chem. 2015, 87, 900-906, Barcode Microchips for
Biomolecular Assays)Using the structure and its coding rule of one-dimensional bar code, according to requirement of experiment by bar code
Each " bar " is designed to microchannel with " sky ", completes the detection of human body inhibition of HIV, and the microchannel can be used for inspection simultaneously
Multiple pathogens are surveyed, finally reacted result is scanned with hand held scanner, and then determines the property of sample.The method has
Consumption agent is few, and passage is novel in design, detects the advantages of flux is high.
The A of Publication No. CN 103272656 discloses a kind of " Bar code micro fluidic chip and purposes ", and the invention is related to one
Micro-fluidic chip is planted, its micro-fluidic chip includes chip unit, and the chip unit includes example reaction passage, in the sample
When there is the chromogenic reaction of sample in reaction channel with the chromogenic reaction that sample does not occur, the reacted bar code figure for being formed
Case can be different characters by bar code reader recognition, so that it is determined that the property of sample.
In order to reach a kind of more convenience-for-people detection architecture, and smart mobile phone become increasingly popular not only brought it is huge
Big facility, while also having attracted the extensive concern of numerous domestic and international researchers.Due to the powerful image procossing of smart mobile phone
Function and open application development environment, many researchers are applied in biological detection.With bar code
Popularization, many researchers are by studying observation, it was found that the architectural feature of bar code, and using this architectural feature as life
Analyte detection passage.Such as Li et al.(J. Guo, J. X. H. Wong, C. E. Cui, X. C. Li and H-Z. Yu,
A smartphone-readable barcode assay for the detection and quantitation of
pesticide residues, Analyst, 2015, 140, 5518-5525)Using 39 yards of "+", "-" structures in bar code
Particularity, by the PDMS passages for designing and homochromy 39 yards of "+" composition " bio-barcode " for printing so that the biological bar
Shape code can be read by mobile phone A PP, reach the purpose of a qualitative detection.And then in order to realize further detecting, Yu etc.
People (J. X. H. Wong, X. C. Li, F. S. F. Liu and H-Z. Yu, Direct Reading of Bona
Fide Barcode Assays for Diagnostics with Smartphone Apps, Scientific Reports
2015,5,11727) using mobile phone A pp, with reference to " the biology that 39 yards of "+", particularity of "-" structure in bar code are constituted
Bar code " realizes the quantitative determination of hCG.The method does not only need expensive instrument, and quickly examining suitable for scene
It is disconnected.
Therefore, with reference to the coding rule of two-dimensional bar, the module that 3D printer is printed is designed to detect reacts the present invention
Vessel, using smart mobile phone program is detected, a kind of self-editing mobile phone A pp that can collect coding, decode, be detected on one realizes life
Change reaction quick detection purpose.
The content of the invention
The invention solves the problems that concrete technical problems be existing biochemical reaction detection method be generally required for costliness specialty
Instrument;And be based on smart mobile phone detecting system, using Quick Response Code pattern as sense channel and incorporate coding rule biochemistry it is anti-
Detection method is answered not to be suggested at present.The module that the purpose of the present invention prints 3D printer is utilized as detection reaction vessels
The characteristics of it can freely dismantle, assemble, combined with intelligent mobile phone detection program realizes quick, the portable detection to biochemical reaction
Method, that is, set up a kind of biological method for quick based on smart phone two-dimensional code style.
The present invention coding rule part of Quick Response Code is dissolved among the design of chip, and write collection coding, decoding,
It is detected on mobile phone A pp of one.
The measure that the present invention is taken is based on the biological method for quick of smart phone two-dimensional code style, methods described
Be by the use of 3D printer print module as detection reaction vessels, the characteristics of can arbitrarily dismantling, assemble using it, with reference to oneself
The mobile phone A pp program write, automatically generates required pattern in 2 D code, is assembled, is spliced according to the pattern for generating, and makes
Detection reaction vessels needed for reaction, then by mobile phone decoding program, are scanned to the result after chromogenic reaction, according to sweeping
Character after retouching is contrasted with blank pattern, determines the property of sample, intercepts reacted picture, and picture is analyzed,
Reach the purpose of quantitative determination.
The detection reaction vessels of present invention design include sample chromogenic reaction and two kinds of sample chromogenic reaction do not occur
Situation, the pattern generated after the reaction be enough to read result by mobile phone A pp.
Add sample that the colour contrast rate after chromogenic reaction occurs in the detection reaction vessels mentioned in the present invention enough
Greatly.
The concrete grammar that its described speed is surveyed is as follows.
Based on the biological method for quick of smart phone two-dimensional code style, the method for quick is by following step
Suddenly carry out:
For the preparation that biological respinse detects vessel;
It is anti-for realizing the colour developing that sample amounts are detected for adding reagent to be detected in the detection reaction vessels
Should;
For the smart mobile phone detection program of biological respinse detection, testing result is shown;
The detection reaction vessels are by 3DMax Software for Design, with the detection that photosensitive resin is printed as material by 3D printer
Reaction vessels;
The detection reaction vessels are freely to be dismantled according to requirement of experiment, assembled.
Further, the method for quick follow these steps to carry out:
(1)It is that, by several size identical module compositions, each module foreign minister is for the preparation of biological detection reaction vessels
It is 3mm that 8mm, width are 8mm, height, and interior length is 7mm, and wide is 7mm, and height is 1 ㎜, moves freely, assembled constitutes a Quick Response Code pattern
Check pattern;
2)For adding reagent to be detected in the biological detection reaction vessels, realize that the colour developing that sample amounts are detected is anti-
Should, it is that sample is equipped with the biological detection reaction vessels, there is the chromogenic reaction of sample in example reaction vessel and do not send out
Under the chromogenic reaction two states of raw sample, the color of example reaction passage is able to be read accordingly by mobile phone reading device
Data result, by compared with results of comparison, the property of sample further being determined according to acquired results, intercepts reacted picture letter
Breath, the picture to obtaining is analyzed, and shows testing result;
(3)The smart mobile phone detection program for biological respinse detection, shows testing result, is write simultaneously by Java language
Operate on Android phone platform.
Further, additional technical feature is as follows.
Example reaction in the detection reaction vessels is same sample reaction or different example reactions, and same detection is reacted
Example reaction detection reagent in vessel is same reaction detection reagent or differential responses detection reagent.
The sample detection reaction vessels are that same sample detects reaction vessels or different sample detection reaction vessels, same
Example reaction in example reaction detection vessel is same sample reaction or different example reactions.
The module is made up of one group of structure, including position sensing area, position sensing marker space, positioning area, correction zone,
Version information area, format information area and data field.
The module of the Quick Response Code is detection reaction vessels region, and remaining module is the non-reaction vessels region of detection.
The detection reaction vessels region is reacted in data field, and it is non-detection reaction not react in data field
Vessel region.
The detection reaction vessels quantity of the Quick Response Code is required and the number of determination detection reaction vessels according to experiment detection
Amount.
The module is made up of the different little module of color according to detection demand each unit.
The fast detection method is to make sample be enough to occur under conditions of chromogenic reaction in detection sample reaction vessels, profit
Passage is distinguished with mobile phone recognition App, the property of sample is determined according to the character of recognition, intercepted after reaction with mobile phone A pp
Picture is obtained, picture is analyzed, realize Quantitative detection.
The above-mentioned biological method for quick based on smart phone two-dimensional code style is realized, the fast determining method is to prepare life
Change reaction detection vessel, its detection reaction vessels is that, by size identical, arbitrarily dismounting, the module of assembling is spliced;In device
Detection reagent is added in ware, chromogenic reaction occurs, reacted result is scanned using mobile phone camera, by intelligent hand
Machine testing program processes and shows the colour developing result after chromogenic reaction.The present invention realizes the quick of biochemical reaction based on smart mobile phone
Detection, it is easy to operate with easy to carry, it is with low cost, the advantages of quick, it is adaptable to field quick detection.
Compared with prior art, this fast determining method embody a concentrated reflection of it is following some:
(1)The generation of two-dimensional bar can be determined according to the demand of detection, and then determine the quantity of detection vessel.
(2)Detection reaction vessels can arbitrarily be dismantled according to requirement of experiment, assembled.
(3)Reagent dosage is few, greatly reduces testing cost.
(4)The colour developing result after program recognition chromogenic reaction is detected using smart mobile phone, a step quick detection is really realized,
Substantially reduce detection time.
(5)Whole detection process only needs a smart mobile phone, i.e., arbitrarily generate experiment institute with self-editing mobile phone A pp
The two-dimensional bar figure for needing, assembles with reference to the module that 3D printer is printed according to the two-dimension code pattern splicing for being generated, and is counting
According to the sample that requirement of experiment is added where the determination in area, there is chromogenic reaction, recycle mobile phone A pp to the knot after chromogenic reaction
Fruit is scanned, and is contrasted with above-mentioned assembled original two dimensional code pattern, detection sample property, intercepts reacted picture letter
Breath, is analyzed to the picture of the acquisition, shows testing result.Above-mentioned technical requirements popularization scope is wide.
Description of the drawings
Fig. 1 is the detection reaction vessels top view printed with 3D printer in the present invention.
Fig. 2 is the detection reaction vessels upward view printed with 3D printer in the present invention.
Fig. 3 is the pattern in 2 D code generated using mobile phone coded program as a example by 03.
Fig. 4 is the pattern in 2 D code after the module assembly printed using 3D printer.
Fig. 5 is the smart mobile phone detection program runnable interface of present invention design.
Fig. 6 is smart mobile phone decoding interface of the present invention.
Fig. 7 is smart mobile phone decoded result of the present invention.
Fig. 8 is preview interface of the present invention.
Fig. 9 is interpretation of result interface of the present invention.
Specific embodiment
Below by taking parathion-methyl in detection fruits and vegetables as an example, as control as a example by 03 pattern generated using Quick Response Code, to this
The specific embodiment of invention is further described in detail.
A kind of above-mentioned the provided smart phone two-dimensional code biology method for quick of the present invention, the quick detection side are provided
Method is comprised the following steps that:
(1)03 character is input into using Encode, the pattern of Quick Response Code pattern is generated.
(2)The preparation of microchannel:Using 3D laser printers, with bright resin as material, by polishing, make outer a length of
8mm, a width of 8 ㎜, a height of 3mm, the example reaction head of district is 7mm, a width of 7 ㎜, a height of 1mm, and base thickness is anti-for the square detection of 1mm
Answer vessel to be spliced into the pattern in 2 D code needed for experiment, make biological respinse vessel.
(3)Parathion-methyl standard curve making:The PBS of configuration 510mL(pH=8), 1mL concentration is taken for 100
The parathion-methyl titer ultra-pure water of g/mL(Buffer solution)10 g/mL are diluted to, volumetric flask are placed in and are kept away in 4 DEG C of refrigerator
Light is preserved, standby.It is dissolved in PBS as mother liquor using the parathion-methyl titer of 10 g/mL, is configured to concentration difference
The L of parathion-methyl solution 200 for 0,0.5,2,4,6 g/mL is placed in brown centrifuge tube, be separately added into 10 L developers and
The mixing of 10 L enzyme liquids shakes up, and is placed in 37 DEG C of insulating box and cultivates 30min.10 L substrates are added in above-mentioned centrifuge tube, timing,
Mixing shakes up.It is each triplicate.The parathion-methyl solution for once taking the above-mentioned variable concentrations of the configuration of 49 L respectively adds step
In microchannel described in rapid 1, scanning and analysis result after 10min.
(4)The detection of parathion-methyl in fruits and vegetables:As a example by parathion-methyl in detect apple.Will be from local supermarket
The apples peeling bought back.Weigh 5g apple epidermises to be dissolved in the phosphate buffer of 10mL, quickly shake up 5min, extract above-mentioned molten
Liquid, is centrifuged 3min in centrifuge, goes 200 μ L of supernatant liquid to add centrifuge tube to be detected as the residual liquid to be checked of agriculture.
(5)Smart mobile phone detects program reading process:Icon is clicked on mobile phone screen and enters main interface, main interface includes
Four buttons, respectively New Test, Decode, Encode, Quit.Click on Decode buttons and enter mobile phone decoding program, show
Show the result after chromogenic reaction, click on return push-button and enter main interface.Click on New Test buttons and enter mobile phone camera preview circle
Face, now can show a pre-set screen interception square alignment box, by the reaction bar after colour developing in preview interface
After band is to dead square bar frame, Capture buttons are clicked on, program can automatically intercept the picture in square-shaped frame and calculate in RGB
B values be saved in mobile phone, then click on Analyses buttons, program can automatically calculate the B values of inframe, and according to methyl pair
The concentration of sulphur phosphorus titer and corresponding B values ratio fit Calibration equation, with reference to this Calibration equation B values meter corresponding with liquid to be checked
The concentration value in parathion-methyl sample in testing sample is calculated, finally testing result is directly displayed on interface.
Claims (8)
1. the biological method for quick of smart phone two-dimensional code style is based on, and the method for quick is to follow these steps to
Carry out:
For the preparation that biological respinse detects vessel;
It is anti-for realizing the colour developing that sample amounts are detected for adding reagent to be detected in the detection reaction vessels
Should;
For the smart mobile phone detection program of biological respinse detection, testing result is shown;
The detection reaction vessels are by 3DMax Software for Design, with the detection that photosensitive resin is printed as material by 3D printer
Reaction vessels;
The detection reaction vessels are freely to be dismantled according to requirement of experiment, assembled.
2. biology method for quick as claimed in claim 1, the biological method for quick is to follow these steps to carry out
's:
(1)It is that, by several size identical module compositions, each module foreign minister is for the preparation of biological detection reaction vessels
It is 3mm that 8mm, width are 8mm, height, and interior length is 7mm, and wide is 7mm, and height is 1 ㎜, moves freely, assembled constitutes a Quick Response Code pattern
Check pattern;
(2)For adding reagent to be detected in the detection reaction vessels, the chromogenic reaction that sample amounts are detected is realized,
It is to be equipped with sample in the detection reaction vessels, the chromogenic reaction of sample occurs in example reaction vessel and sample does not occur
Under chromogenic reaction two states, the color of example reaction passage is able to read corresponding data knot by mobile phone reading device
Really, by compared with results of comparison, the property of sample further being determined according to acquired results, reacted pictorial information is intercepted, it is right
The picture of acquisition is analyzed, and shows testing result;
(3)The smart mobile phone detection program for biological respinse detection, shows testing result, is write simultaneously by Java language
Operate on Android phone platform.
3. biology method for quick as claimed in claim 1, the example reaction of the detection reaction vessels is same sample
Reaction or different example reactions, the example reaction detection reagent in same reaction vessels is same reaction detection reagent or different anti-
Answer detection reagent.
4. biology method for quick as claimed in claim 1, the detection reaction vessels are that same sample detects reactor
Ware or different sample detection reaction vessels.
5. biology method for quick as claimed in claim 1 or 2, the module be by each unit according to detection demand by
The different little module of color is constituted, including position sensing area, position sensing marker space, positioning area, correction zone, version information area,
Format information area and data field.
6. biology method for quick as claimed in claim 1, the detection reaction vessels quantity of the Quick Response Code is according to inspection
Survey and require what is determined;The module section of the Quick Response Code is conversion zone, and remaining module is non-conversion zone.
7. detection reaction vessels as claimed in claim 1 can freely be dismantled according to requirement of experiment, assembled.
8. biology method for quick as claimed in claim 1 or 2, the fast detection method is to make sample anti-in sample detection
To answer be enough in vessel and occur under conditions of chromogenic reaction, the pattern after chromogenic reaction is distinguished using mobile phone A pp, according to knowledge
The character of reading determines the property of sample, is intercepted with mobile phone A pp and obtain after reaction picture, and picture is analyzed, and realizes fast quantification
Detection.
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Inventor after: Zhang Xiaoliang Inventor after: Li Xiaochun Inventor after: Gao Qin Inventor after: Zhang Yuhang Inventor after: Yu Huazhong Inventor before: Li Xiaochun Inventor before: Gao Qin Inventor before: Zhang Xiaoliang Inventor before: Yu Huazhong |
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GR01 | Patent grant | ||
GR01 | Patent grant |