CN106632708B - Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method - Google Patents
Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method Download PDFInfo
- Publication number
- CN106632708B CN106632708B CN201610121973.XA CN201610121973A CN106632708B CN 106632708 B CN106632708 B CN 106632708B CN 201610121973 A CN201610121973 A CN 201610121973A CN 106632708 B CN106632708 B CN 106632708B
- Authority
- CN
- China
- Prior art keywords
- alcohol
- polysaccharide
- dendrobium devonianum
- homogeneous polysaccharide
- thick many
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- High Energy & Nuclear Physics (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Since dendrobium devonianum polysaccharide viscosity is high, high activity polysaccharide segment molecule amount is big, thus its isolate and purify and structure elucidation in terms of there are certain difficulty.It thus the invention discloses a kind of method of separating-purifying dendrobium devonianum homogeneous polysaccharide, is first separated with substep alcohol precipitating method, then through gel column purification, the homogeneous polysaccharide of pure dendrobium devonianum can be obtained.In addition, the present invention also identifies the skeleton structure of dendrobium devonianum homogeneous polysaccharide using multispectral parse, can primary structure precise Identification to the polysaccharide, specifying the main polysaccharide in dendrobium devonianum is acetyl group glucomannans.
Description
Technical field
This case is related to dendrobium polysaccharide field more particularly to a kind of method for isolating and purifying dendrobium devonianum polysaccharide, and to this
The method of polysaccharide progress primary structure identification.
Background technique
Polysaccharide is important bioactive macromolecule, is improved immunity, anti-inflammatory, anti-aging, anti-oxidant and other effects.It is more
A large amount of hydrophilic hydroxy groups that sugar is linked, make it have strong absorptive, emulsibility, high viscosity.
Dendrobium nobile is first of nine big mesonas, and dendrobium devonianum polysaccharide has preferable raising immunity, antitumor and other effects, polysaccharide
Rich content, up to 35-42%.It is found through experiments that early period, dendrobium devonianum polysaccharide is antitumor, mention compared with ganoderma lucidum polysaccharide
High efficacy of immunity is more significant.The extracting method of dendrobium polysaccharide mainly uses water extraction, reflux extraction, ultrasound at present
Wave/microwave loss mechanisms, enzyme extraction method, ultrahigh-pressure extraction method etc..But since dendrobium devonianum polysaccharide viscosity is high, high activity polysaccharide section point
Son amount is big, isolate and purify and structure elucidation in terms of there are certain difficulty.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of separating-purifying dendrobium devonianum homogeneous polysaccharide, utilize water extract-alcohol precipitation point
From means such as, gel column purifications, the homogeneous polysaccharide of dendrobium devonianum can be obtained.
Another object of the present invention identifies the skeleton structure of dendrobium devonianum homogeneous polysaccharide using multispectral parsing.
In order to achieve the above object, the invention adopts the following technical scheme:
The method of separating-purifying dendrobium devonianum homogeneous polysaccharide is first divided water extract-alcohol precipitation Thick many candies with substep alcohol precipitating method
From obtaining the homogeneous polysaccharide of dendrobium devonianum then through gel column purification.
Wherein carrying out separation using substep alcohol precipitating method includes: to redissolve Thick many candies in water, adds alcohol to 50% progress second
Alcohol alcohol precipitation, obtains sediment, which is removed, and continues plus alcohol is to system alcohol 70%, obtain flocculent deposit, this is cotton-shaped
It is precipitated as 50-70% alcohol precipitation Thick many candies segment.The purity of 50-70% alcohol precipitation Thick many candies segment is 90% or more.
Include: to redissolve 50-70% alcohol precipitation Thick many candies segment in water using gel column purification, purified with gel column,
With Phenol-sulphate acid method tracking and monitoring collect, be concentrated by evaporation, dialyse, freeze-drying, obtain gel column separating purification cross it is uniform more
Sugar.Wherein preferably purified using superdex-200 gel column；It is preferred that collecting symmetrical peak 33-45 pipe；It is preferred that using rotation
Evaporimeter concentration, concentration time 40-80 minutes.
Following technique can be used in water extract-alcohol precipitation Thick many candies: dendrobium devonianum being decocted in water, and is separated by solid-liquid separation, gained
Liquid is Aqueous extracts；Ethyl alcohol alcohol precipitation is added into Aqueous extracts, makes concentration of alcohol 70%-80% in system, obtains Thick many candies.
The present invention also provides a kind of primary structure identification methods of dendrobium devonianum homogeneous polysaccharide:
Homogeneous polysaccharide is measured with HPGPC analysis instrument, high performance liquid chromatograph, evaporative light scattering detector ELSD first
Molecular weight is 1.94 × 105Da；
Then homogeneous polysaccharide is methylated and is derived, compareed using gas chromatograph-mass spectrometer (GC-MS) with monosaccharide standard, measured
Monosaccharide composition；Mannose in monosaccharide composition: the molar ratio of glucose is 0.77:0.23；
Glycosidic bond → 4 are inferred finally by nmr analysis)-man- (1 →, → 4)-Glc- (1 → and → 4) -2-o-
Acetyl-man- (1 → ratio are as follows: 1.46:1.74:1.00.Nuclear magnetic spectrogram structural analysis is as follows:
According to carbon spectrum signal, the anomeric carbon signals peak of dendrobium polysaccharide is that δ 101.3 can be determined by de pt135 map
The signal peak of C6 is δ 61.7；The chemical shift of C2, C3, C4, C5 are distributed in the region 70~80ppm of δ；77.76 signal peak of δ is answered
C4 is belonged to, the on-link mode (OLM) for thus deducing dendrobium polysaccharide may be Isosorbide-5-Nitrae-Man；
In addition, having signal peak in δ 20.3-21.0 and δ 172.9-173.9, the methyl and carbonyl letter of acetate should be belonged to
Number, thus infer in the polysaccharide structures of dendrobium polysaccharide, C2, the corresponding hydroxyl of C3 may be replaced by acetate；
As can be seen that the anomeric carbon signals peak of glucose glycoside should be distributed in 101.2/4.7 in HSQC map, and C4 believes
Number peak should be 79.1/4.07, and the chemical potential of other C6 is dispersed with 61-62.3ppm, C2, C3, and the chemical shift of C5 should be distributed in
Between 70-77ppm, dendrobium polysaccharide presence → 4 are thus inferred)-Glc- (1 → glycosidic bond；
There is signal peak to be integrated δ 99-103, the δ 20.3-21.0 and δ 172.9-173.9 of carbon spectrum, available three
The integral ratio of peak-to-peak signal are as follows: 1.02:1.12:3.22, since δ 99-103 is the different region Tou Feng, integrated value should be → 4)-man-
(1 →, → 4) -2-o-acetyl-man- (1 → and → 4)-Glc- (summation of 1 → glycosidic bond, and δ 20.3-21.0 and δ
172.9-173.9 is → 4) and -2-o-acetyl-ma n- (the distinctive signal peak of 1 → glycosidic bond, and ratio (1.02:1.12 :) connects
It is bordering on 1:1, and the ratio of mannose and glucose is 0.77:0.23, it can therefore be concluded that glycosidic bond → 4 out)-man- (1 →,
→ 4)-Glc- (1 → and → 4) -2-o-acetyl-man- (1 → ratio answer are as follows: 1.46:0.74:1.00.
It can purify to obtain pure homogeneous polysaccharide by the above method, substep alcohol precipitation can replace cellulose column point completely
From, reach good effect, saves resource, it is time saving and energy saving；And can primary structure precise Identification to the polysaccharide, specify tooth
Main polysaccharide in valve dendrobium nobile is acetyl group glucomannans.
Detailed description of the invention
Fig. 1 is sample HPGPC figure；
Fig. 2 is monosaccharide standard spectrogram；
Fig. 3 is simple monosaccharide composition figure；
Fig. 4 is sample1H NMR spectra；
Fig. 5 is sample13C NMR spectra；
Fig. 6 is sample hsqc spectrum figure.
Specific embodiment
The present invention is further described with relevant drawings combined with specific embodiments below, the advantages and features of the present invention will
It is apparent with description.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.This field
Technical staff should be understood that without departing from the spirit and scope of the invention can details to technical solution of the present invention and
Form is modified or is replaced, but these modifications and replacement are fallen within the protection scope of the present invention.
Embodiment 1: passing through following steps separating-purifying dendrobium devonianum homogeneous polysaccharide: dendrobium devonianum decocted in water, and
It is separated by solid-liquid separation, obtains Aqueous extracts；Ethyl alcohol alcohol precipitation is added in Aqueous extracts makes concentration of alcohol 70% in system, stands
Night obtains Thick many candies；By Thick many candies redissolve in water, completely dissolve in water polysaccharide, add alcohol to 50% carry out ethyl alcohol alcohol precipitation,
Sediment is obtained, which is removed, continues plus alcohol is to system alcohol 70%, obtain the 50-70% of 90% or more purity
Alcohol precipitation Thick many candies segment；Thick many candies segment is redissolved in water, is purified with superdex-200, is tracked and is supervised with Phenol-sulphate acid method
It surveys and collects, collect symmetrical peak 33-45 pipe, be concentrated 50 minutes using Rotary Evaporators, dialysed, freeze-drying obtains gel column point
From the homogeneous polysaccharide purified.
Embodiment 2: passing through following steps separating-purifying dendrobium devonianum homogeneous polysaccharide: dendrobium devonianum decocted in water, and
It is separated by solid-liquid separation, obtains Aqueous extracts；Ethyl alcohol alcohol precipitation is added in Aqueous extracts makes concentration of alcohol 80% in system, stands
Night obtains Thick many candies；By Thick many candies redissolve in water, completely dissolve in water polysaccharide, add alcohol to 50% carry out ethyl alcohol alcohol precipitation,
Sediment is obtained, which is removed, continues plus alcohol is to system alcohol 70%, obtain the 50-70% of 90% or more purity
Alcohol precipitation Thick many candies segment；Thick many candies segment is redissolved in water, is purified with superdex-200, is tracked and is supervised with Phenol-sulphate acid method
It surveys and collects, collect symmetrical peak 35-42 pipe, be concentrated 50 minutes using Rotary Evaporators, dialysed, freeze-drying obtains gel column point
From the homogeneous polysaccharide purified.
Embodiment 3: structure elucidation is carried out as following steps homogeneous polysaccharide to obtained by:
Molecular weight determination: it with HPGPC analysis instrument, high performance liquid chromatograph (U.S. A gilent1100series), steams
Light Scattering Detector ELSD measurement, by sample preparation at 2mg/ml, sample volume 10ul.Fig. 1 is the HPGPC figure of gained sample.
With the glucans of different relative molecular masses (Mw1270,5220,11600,48600,80900,147600,273000,
409800) standard items are used as, standard curve is made, measures the purity and relative molecular mass of polysaccharide, finally obtain homogeneous polysaccharide
Molecular weight is 1.94 × 105Da。
Monosaccharide composition measuring: homogeneous polysaccharide is methylated and is derived, gas chromatograph-mass spectrometer (GC-MS) and monosaccharide standard are utilized
Control, wherein monosaccharide standard spectrogram is shown in that Fig. 2, simple monosaccharide composition figure are shown in Fig. 3, is compareed by two figures, obtains mannose: glucose
(molar ratio) are as follows: 0.77:0.23.
Derived from homogeneous polysaccharide methylation specifically comprises the processes of: by the 2M trifluoroacetic acid hydrolysis 90min of polysaccharide 2mg 1ml, rotation
Turn evaporimeter to be evaporated, 2ml methanol is added, is evaporated, 2 times repeatedly.2ml distilled water is added in residue, and 60mg sodium borohydride reduction 8 is small
When, glacial acetic acid is added and neutralizes, methanol 3ml is added, 3 times repeatedly, then 1ml second is added in revolving to powder, 110 degree of baking oven drying
Acid anhydrides acetylation.100 DEG C of reaction 1h, it is cooling, 3mL toluene is then added, reduced pressure is evaporated, and is repeated 4-5 times, extra to remove
Aceticanhydride.It is transferred to separatory funnel after product after acetylation is dissolved with 3mL chloroform, a small amount of distilled water is added and fullys shake
Afterwards, upper layer aqueous solution is removed, is so repeated 4 times.Chloroform layer is dry with suitable anhydrous sodium sulfate, is settled to 10mL and waits for GC-MS
Analysis.
Nmr analysis: it is inferred to glycosidic bond → 4)-man- (1 →, → 4)-Glc- (1 → and → 4) -2-o-acetyl-
Man- (1 → ratio answer are as follows: 1.46:1.74:1.00.In conjunction with Fig. 4-6, specific nuclear magnetic spectrogram structural analysis is as follows:
According to the carbon spectrum signal of Fig. 5, the anomeric carbon signals peak of dendrobium polysaccharide is δ 101.3, can be with by dept135 map
The signal peak for determining C6 is δ 61.7.The chemical shift of C2, C3, C4, C5 are distributed in the region 70~80ppm of δ.77.76 signal of δ
Peak should belong to C4, since in C4 substitution occurs for O- and cause to migrate to low field.Therefore, the link of dendrobium polysaccharide can be deduced
Mode may be 1,4-Man.
In addition, having signal peak in δ 20.3-21.0 and δ 172.9-173.9, the methyl and carbonyl letter of acetate should be belonged to
Number.It can therefore be concluded that the corresponding hydroxyl of C2, C3 may be replaced by acetate in the polysaccharide structures of dendrobium polysaccharide.
Result is formed by monosaccharide it is found that dendrobium polysaccharide by mannose and glucose group at (molar ratio 77:23), due to Portugal
The ratio that grape sugar occupies is fewer, and is difficult to observe the signal of glucose glycoside key in carbon spectrum, and in the HSQC map of Fig. 6
In, it can see some signals reluctantly, the anomeric carbon signals peak of glucose glycoside should be distributed in 101.2/4.7, and C4 signal peak
It should be 79.1/4.07, the chemical potential of other C6 is dispersed with 61-62.3ppm, C2, C3, and the chemical shift of C5 should be distributed in 70-
Between 77ppm.It could therefore be concluded that dendrobium polysaccharide presence → 4)-Glc- (1 → glycosidic bond.
Using bruker Topspin3.0 software, have to δ 99-103, the δ 20.3-21.0 and δ 172.9-173.9 of carbon spectrum
Signal peak is integrated, the integral ratio of available three peak-to-peak signals are as follows: 1.02:1.12:3.22, since δ 99-103 is different
The region Tou Feng, integrated value should be → 4)-man- (1 →, → 4) -2-o-acetyl-man- (1 → and → 4)-Glc- (1 → glucosides
The summation of key, and δ 20.3-21.0 and δ 172.9-173.9 are → 4) -2-o-acetyl-man- (distinctive signal of 1 → glycosidic bond
Peak, and ratio (1.02:1.12 :) is close to 1:1, and the ratio of mannose and glucose is 0.77:0.23, it can therefore be concluded that
Glycosidic bond → 4 out)-man- (1 →, → 4)-Glc- (1 → and → 4) -2-o-acetyl-man- (1 → ratio answer are as follows: 1.46:
0.74:1.00。
Claims (4)
1. a kind of method of separating-purifying dendrobium devonianum homogeneous polysaccharide, it is characterised in that water extract-alcohol precipitation Thick many candies are first used substep alcohol
Heavy method is separated, and then through gel column purification, obtains the homogeneous polysaccharide of dendrobium devonianum；
Wherein, carrying out separation using substep alcohol precipitating method includes: to redissolve Thick many candies in water, adds alcohol to 50% progress ethyl alcohol alcohol
It is heavy, sediment is obtained, which is removed, continues plus alcohol is to system alcohol 70%, obtain flocculent deposit, the flocculent deposit
For 50-70% alcohol precipitation Thick many candies segment；
Gel column purification includes: to redissolve 50-70% alcohol precipitation Thick many candies segment in water, is purified with gel column, with sulfuric acid benzene
Phenol method tracking and monitoring is collected, and is concentrated by evaporation, and is dialysed, and freeze-drying obtains the homogeneous polysaccharide that gel column separating purification is crossed；
Glycosidic bond → 4 of purified dendrobium devonianum homogeneous polysaccharide are inferred by nmr analysis)-man- (1 →, → 4)-Glc- (1 →
With → 4) -2-o-acetyl-man- (1 → ratio are as follows: 1.46:1.74:1.00, the main polysaccharide in dendrobium devonianum are acetyl
Base glucomannans；
The molecular weight of the homogeneous polysaccharide is 1.94 × 105Da；
Its monosaccharide molar ratio composition are as follows: mannose: glucose=0.77:0.23.
2. the method for separating-purifying dendrobium devonianum homogeneous polysaccharide as described in claim 1, which is characterized in that the 50-70% alcohol
The purity of heavy Thick many candies segment is 90% or more.
3. the method for separating-purifying dendrobium devonianum homogeneous polysaccharide as described in claim 1, it is characterised in that use superdex-
200 gel columns are purified；Collect symmetrical peak 33-45 pipe；It is concentrated using Rotary Evaporators, concentration time 40-80 minutes.
4. the method for separating-purifying dendrobium devonianum homogeneous polysaccharide as described in claim 1, it is characterised in that water extract-alcohol precipitation Thick many candies
It include: to decoct dendrobium devonianum in water, and be separated by solid-liquid separation, gained liquid is Aqueous extracts；Ethyl alcohol is added into Aqueous extracts
Alcohol precipitation makes concentration of alcohol 70%-80% in system, obtains Thick many candies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610121973.XA CN106632708B (en) | 2016-03-03 | 2016-03-03 | Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610121973.XA CN106632708B (en) | 2016-03-03 | 2016-03-03 | Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106632708A CN106632708A (en) | 2017-05-10 |
| CN106632708B true CN106632708B (en) | 2019-07-30 |
Family
ID=58848666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610121973.XA Active CN106632708B (en) | 2016-03-03 | 2016-03-03 | Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106632708B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107188980A (en) * | 2017-05-24 | 2017-09-22 | 中国科学院上海药物研究所 | The different xylans of β 1,4, its sulfated derivative and its production and use |
| CN108956676B (en) * | 2018-04-08 | 2020-12-15 | 温州医科大学 | Dendrobium officinale powder adulteration ratio detection method based on nuclear magnetic resonance characteristic spectrum |
| CN108530554A (en) * | 2018-05-23 | 2018-09-14 | 劲牌有限公司 | It is soluble in the polysaccharide and preparation method thereof of wine, health liquor and preparation method thereof |
| CN109400742B (en) * | 2018-11-09 | 2021-10-29 | 浙江省医学科学院 | Dendrobium devonianum refined polysaccharide and preparation method and application thereof |
-
2016
- 2016-03-03 CN CN201610121973.XA patent/CN106632708B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| Purification, composition analysis and antioxidant activity of the polysaccharides;AoXue Luo et.al,;《Carbohydrate Polymers》;20091021;第79卷;pages 1014-1019 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106632708A (en) | 2017-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106632708B (en) | Dendrobium devonianum homogeneous polysaccharide isolate and purify and primary structure identification method | |
| Bai et al. | Structural elucidation, anti-inflammatory activity and intestinal barrier protection of longan pulp polysaccharide LPIIa | |
| Hua et al. | Structural characterization and DPPH· radical scavenging activity of a polysaccharide from Guara fruits | |
| Wang et al. | Structural characterization and in vitro antitumor activity of polysaccharides from Zizyphus jujuba cv. Muzao | |
| Liu et al. | Brönsted acidic ionic liquid based ultrasound-microwave synergistic extraction of pectin from pomelo peels | |
| Capek et al. | Characterization of immunomodulatory polysaccharides from Salvia officinalis L. | |
| Boucelkha et al. | Production of guluronate oligosaccharide of alginate from brown algae Stypocaulon scoparium using an alginate lyase | |
| Zheng et al. | Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity | |
| Huo et al. | Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells | |
| Huang et al. | Structural characterization and osteoprotective effects of a novel oligo-glucomannan obtained from the rhizome of Cibotium barometz by alkali extraction | |
| CN108727509B (en) | Moso bamboo shoot shell arabinogalactan and preparation and application thereof | |
| CN102277398B (en) | Process for preparing modified pectin with high bioavailability and antitumor application of modified pectin | |
| Zhu et al. | Purification, in-depth structure analysis and antioxidant stress activity of a novel pectin-type polysaccharide from Ziziphus Jujuba cv. Muzaoresidue | |
| US11474100B2 (en) | Chromophore-labeled oligosaccharide markers and methods of use thereof | |
| Sudharsan et al. | Isolation, characterization and bioactive potential of sulfated galactans from Spyridia hypnoides (Bory) Papenfuss | |
| CN102675483B (en) | Homogeneous pectic polysaccharides and method for obtaining same from tea polysaccharides | |
| CN114591448A (en) | Phellinus igniarius sporophore mannogalactan and preparation and application thereof | |
| Cao et al. | Structural elucidation of an active polysaccharide from Radix Puerariae lobatae and its protection against acute alcoholic liver disease | |
| CN103613684B (en) | A kind of isolation and purification method of N-acetylation shell six sugar of different acetyl degree | |
| Sui et al. | Removal and recovery of deep eutectic solvent with membrane-based methodology: A promising strategy to enhance extraction and purification of Dendrobium officinale flavonoids | |
| Zhang et al. | Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin | |
| Guo et al. | Structural and functional characteristics of pectins from three cultivars of apple (Malus pumila Mill.) pomaces | |
| CN102827305A (en) | Pleurotus eryngii polysaccharide capable of reducing accumulation of foam cell lipid and preparation method thereof | |
| CN106589158A (en) | Helicteres angustifolia polysaccharide and preparation method and application thereof | |
| Chen et al. | Structural characterization, molecular dynamic simulation, and conformational visualization of a water-soluble glucan with high molecular weight from Gastrodia elata Blume |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |