CN106631799A - Method for extracting chlorogenic acid from honeysuckle - Google Patents
Method for extracting chlorogenic acid from honeysuckle Download PDFInfo
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- CN106631799A CN106631799A CN201611162882.7A CN201611162882A CN106631799A CN 106631799 A CN106631799 A CN 106631799A CN 201611162882 A CN201611162882 A CN 201611162882A CN 106631799 A CN106631799 A CN 106631799A
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- chlorogenic acid
- honeysuckle
- ionic liquid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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Abstract
The invention discloses a method for extracting chlorogenic acid from honeysuckle by utilizing ionic liquid ultrasonic enzymolysis. According to the method, honeysuckle is taken as a raw material, the ionic liquid is added in an extraction process and then an ultrasound-enzyme coupling method is adopted for acquiring the product. The method comprises the following steps: taking the honeysuckle as the raw material; adding the honeysuckle powder into the ionic liquids in different concentrations; setting ultrasonic temperature at 45 DEG C, enzyme dosage of 0.5-2% and ratio of material to liquid at (1:30)-(1:50); adjusting system pH to 3-5, ultrasonic power to 60-100W and ultrasonic time to 30min-60min; after the ending of extraction, filtering an extracting liquid; recycling ethyl alcohol; re-extracting with ethyl acetate and recycling the ionic liquid; and concentrating, thereby acquiring a raw extract of chlorogenic acid. The method has the advantages of short extraction time, high extraction efficiency, low extraction temperature, recyclable ionic liquid, and the like.
Description
Technical field
The invention belongs to biological technical field, relates to the use of the system extraction acquisition Chlorogenic Acid of Flos Lonicerae crude product of complexity
Method.Specifically, it is related to the method for ionic liquid ultrasound enzymolysis and extraction Chlorogenic Acid of Flos Lonicerae.
Background technology
Honeysuckle is the bud of caprifoliaceae plant, sweet in flavor and cold in property, with clearing heat and detoxicating, anti-inflammation, the carbuncle that disappears dissipate it is swollen and
The effect of hepatic cholagogic.Honeysuckle is distributed more widely in China, and medicinal history is long, and be that health ministry promulgates in the first batch is both food
Product are again one of 60 kinds of Chinese medicines of medicine.
Contain chlorogenic acid, chromocor compound and linalool isoreactivity composition in honeysuckle, wherein, chlorogenic acid is in honeysuckle
Main active, by internationally recognized for " plant gold ".And, chlorogenic acid is because with anti-inflammation, hepatic cholagogic, delaying
Various effects such as aging, are widely applied to the industries such as food, medicine, cosmetics, so extracting separating chlorogenic acid from plant
It is significant.
The liquid that ionic liquid is made up of the larger organic cation of inorganic acid radical anion and volume.It has excellent
Chemical property, such as steam forces down, and heat endurance is good within the scope of wider temperature, adjustable physicochemical properties etc. because
Liquid is presented under room temperature or close room temperature, is a class green solvent of rising in recent years also known as ionic liquid at room temperature.
There are ester bond, 3 l fractions of unsaturated double-bond and polyhydric phenols in the molecular structure of chlorogenic acid.In existing extraction skill
In art, the extractive technique means for generally adopting are for water extraction and organic solvent extraction.Wang Qian etc. [Wang Qian, Li Zhi, He Qi,
Deng. the research [J] of Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves extraction and separation process condition. ion exchange and absorption, 2008,24 (1): 73-
80.] using water as Extraction solvent, and water extraction conditions are optimized, are as a result shown, the recovery rate of chlorogenic acid is 1.48%.
Xiao Wen equalitys [Xiao Wenping, Li Juan. the extraction of Chlorogenic Acid of Flos Lonicerae and assay [J]. Agriculture of Anhui science, 2011,
39(35):21675-21676.] factor of impact Chlorogenic Acid of Flos Lonicerae recovery rate is investigated using ethanol refluxing process, and optimize
Extraction technique, as a result shows, the recovery rate of chlorogenic acid is 3.81%.Traditional chlorogenic acid extraction method shows extraction
The relatively low problem of rate, in addition, the of a relatively high temperature of long-time is possible to that the decomposition of chlorogenic acid can be caused, so as to cause separation pure
Chemical conversion increase originally.Therefore, new extraction separation method also needs appearance badly, and various new extraction sides have newly been risen in recent years
Method.For example, plum forests etc. [plum forests. Chlorogenic Acid of Flos Lonicerae Extraction Processes optimization [J]. Guangzhou chemistry, 2007,32
(4):30-34.] using enzyme process optimize honeysuckle in chlorogenic acid extraction process and drawn optimum extraction condition, as a result show
Show, chlorogenic acid yield is 3.57%.Liu Yamin et al. [Liu Yamin, Liu Yumin, Li Qiong, etc. ultrasonic assistant extracts mountain
Honeysuckle flower chlorogenic acid technique and its Study of Antioxidation [J]. Xu Wenjing et al. [Xu W J, Zhai J W, Cui Q, et
al. Ultra-turrax based ultrasound-assisted extraction of five organic acids
from honeysuckle (Lonicera japonica Thunb.) and optimization of extraction
process[J]. Separation and Purification Technology, 2016, 166:73-82.] using super
Chlorogenic acid in sound assisted extraction honeysuckle, optimizes extraction extraction process, and has drawn optimized parameter, as a result shows, green original
The extraction rate reached of acid is to 5.26%.Zhang Lijin et al. [Zhang L J, Liu J F, Zhang P P, et al.
Ionic liquid-based ultrasound-assisted extraction of chlorogenic acid from
Lonicera japonica Thunb[J]. Chromatographia, 2011, 73(1-2):129-133.] using hydrophilic
Property ionic liquid [BMIM] [BF4] chlorogenic acid in ultrasonic extraction honeysuckle, particle diameter is investigated, [BMIM] [BF4] concentration, pH carries
Take 6 factors such as temperature, ultrasonic power, ultrasonic time and draw optimal procedure parameters, as a result show：Chlorogenic acid under the method
Recovery rate is 5.29%, and more single ultrasonic method improves 37.9%, and compared with ethanol refluxing process 18.8% is improve.Highly polar ionic liquid
Body has stronger dissolving and extractability to natural products, and number of types of ionic liquid has stable to enzyme and activates
Effect, by ionic liquid, enzyme and ultrasonic triplicity, for the extraction of Chlorogenic Acid of Flos Lonicerae, development is a kind of more for the present invention
Efficient new method for extracting.
The content of the invention
The technical problem to be solved in the invention is the recovery rate for lifting extracting chlorogenci acid from honeysuckle in prior art, is dropped
The cost of low extraction, reduces the pollution in extraction process.
In order to solve the technical problem, the technical scheme is that：
A kind of method of the chlorogenic acid extracting from honeysuckle, wherein, the method is concretely comprised the following steps：
（1）Honeysuckle is smashed, in adding ionic liquid, mixture is uniformly mixed so as to obtain；
（2）Ethanol, 0.5-2% are added in the mixture（Account for the proportion of honeysuckle）Enzyme, and adjust mixture pH value be 3-5；
（3）Ultrasound to extraction terminates；
（4）Filter and obtain filtrate；
（5）First reduced pressure concentration simultaneously reclaims ethanol, then with ethyl acetate back extraction chlorogenic acid and reclaims ionic liquid, is concentrated to give green
Ortho acid crude extract.
Method of the present invention, wherein, the ionic liquid be methylimidazole villaumite, 1- butyl -3- ethyl imidazol(e) chlorine
Salt, 1- butyl -3- ethyl imidazol(e) formates, the one kind in 1- ethyl-3-methylimidazole acetate.
Method of the present invention, wherein, concentration of the ionic liquid in extraction system is 0.1-1.5mol/L.
Method of the present invention, wherein, the concentration of alcohol is 70%.
Method of the present invention, wherein, the enzyme dosage is the 0.5%-2% of honeysuckle dry weight.
Method of the present invention, wherein, described ultrasonic temperature is 45 DEG C, and ultrasonic time is 30-60min, ultrasonic work(
Rate is 60-100W.
Method of the present invention, wherein, step（5）In, first reduced pressure concentration reclaims ethanol, then ethyl acetate is added into filter
In liquid, treat to be separately recovered process after system layering, remove layer reduced pressure concentration and reclaim ionic liquid, take upper strata reduced pressure concentration and obtain green
Ortho acid crude extract.
Method of the present invention, wherein, the enzyme is pectase or cellulase.
Method of the present invention, wherein, the ratio of the honeysuckle dry weight and ethanol volume is 1:30-50.
In a preferred embodiment of the invention：
Using the method for ionic liquid ultrasonic extraction Chlorogenic Acid of Flos Lonicerae, comprise the steps：Honeysuckle powder is broken into powder
Boiling flask is placed, adds solid-liquid ratio to be 1:30-1:50 volumetric concentration is 70% ethanol, and mass concentration is 0.5-2%（Gold and silver
Flower dry weight）Pectase or cellulase, and add ionic liquid so that ionic liquid is dense in final extraction system
Spend for 0.1-1.5mol/L, regulation system pH is 3-5；45 DEG C of ultrasonic temperature, ultrasonic power is ultrasound in the equipment of 60-100 W
Time 30min-60min, filters extract, and concentration and recovery ethanol adds ethyl acetate in filtrate, treats to divide after system layering
Do not recycle, remove layer reduced pressure concentration and reclaim ionic liquid, take upper strata reduced pressure concentration and obtain final product chlorogenic acid crude extract.
The beneficial effects of the present invention is
（1）The present invention is using the chlorogenic acid in ionic liquid assisting ultrasonic Enzymatic Extraction honeysuckle, and extraction time is shorter, extracts effect
Rate has reported that document increases more.
（2）Present invention process is simple, mild condition, energy-conserving and environment-protective, solvent recoverable.
Specific embodiment
Embodiment 1：Ultrasonic method chlorogenic acid extracting
After the size-reduced machine of honeysuckle is crushed, sieve, dry, weigh Honeysuckle Flower 2g, be 1 in solid-liquid ratio:50, concentration of alcohol is
70%, system pH is 5, and ultrasonic temperature is 45 DEG C, and ultrasonic power is 180W, and ultrasonic time is to extract green original under conditions of 60min
Acid.Then filter, filter residue is the Honeysuckle Flower after extracting, filtrate adds in ethyl acetate Jing after ethanol is reclaimed in vacuum distillation
In filtrate, process respectively after system layering, remove layer reduced pressure concentration and reclaim ionic liquid, take upper strata reduced pressure concentration and be green original
Sour crude extract, with 0.45 μm of filtering with microporous membrane chlorogenic acid crude extract, is detected under HPLC, is as a result shown under the conditions of being somebody's turn to do
Chlorogenic acid yield can reach 4.73%.
Embodiment 2：Method of pectinase chlorogenic acid extracting
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, be 1 in solid-liquid ratio:50, ethanol is dense
Spend for 70%, pectin enzyme dosage is 1%, system pH is 5, heating-up temperature is the 60min chlorogenic acid extractings that flow back under conditions of 50 DEG C.So
After filter, filter residue be extract after Honeysuckle Flower, filtrate Jing vacuum distillation reclaim ethanol after, ethyl acetate is added into filtrate
In, process respectively after system layering, remove layer reduced pressure concentration and reclaim an ionic liquid, taking upper strata reduced pressure concentration, to be chlorogenic acid thick
Extract, with 0.45 μm of filtering with microporous membrane after, detected under HPLC, as a result show should under the conditions of chlorogenic acid yield can
To reach 4.20%.
3 ultrasounds of embodiment-cellulose enzyme process chlorogenic acid extracting
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 1 in solid-liquid ratio:50, cellulase consumption is 0.8%, and system pH is 5, and ultrasonic power is 150W, ultrasound
Time is chlorogenic acid extracting under conditions of 50min.Then filter, filter residue is the Honeysuckle Flower after extracting, filtrate Jing decompressions are steamed
After evaporating recovery ethanol, ethyl acetate is added in filtrate, processed respectively after system layering, removed layer reduced pressure concentration and reclaim ion
Liquid, takes upper strata reduced pressure concentration and is chlorogenic acid crude extract, with 0.45 μm of filtering with microporous membrane after, examined under HPLC
Survey, as a result show that chlorogenic acid yield can reach 5.73% under the conditions of being somebody's turn to do.
4 ultrasounds of embodiment-pectase Enzymatic Extraction chlorogenic acid
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 1 in solid-liquid ratio:50, pectin enzyme dosage is 1.2%, and system pH is 4, and ultrasonic power is 120W, when ultrasonic
Between for chlorogenic acid extracting under conditions of 40min.Then filter, filter residue be extract after Honeysuckle Flower, filtrate Jing vacuum distillations
After reclaiming ethanol, ethyl acetate is added in filtrate, processed respectively after system layering, removed layer reduced pressure concentration and reclaim ionic liquid
Body, takes upper strata reduced pressure concentration and is chlorogenic acid crude extract, with 0.45 μm of filtering with microporous membrane chlorogenic acid crude extract, under HPLC
Detected, as a result shown that chlorogenic acid yield can reach 6.01% under the conditions of being somebody's turn to do.
5 ultrasounds of embodiment-method of pectinase chlorogenic acid extracting（Methylimidazole villaumite）
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 0.6mol/L in ionic liquid methylimidazole villaumite, and solid-liquid ratio is 1:40, pectin enzyme dosage is 0.5%,
System pH is 4, and ultrasonic power is ultrasonic 50min under conditions of 90W.Then filter, filter residue is the Honeysuckle Flower after extracting,
Filtrate adds ethyl acetate in filtrate Jing after ethanol is reclaimed in vacuum distillation, processes respectively after system layering, removes layer decompression
Concentration and recovery ionic liquid, takes upper strata reduced pressure concentration and is chlorogenic acid crude extract, with 0.45 μm of filtering with microporous membrane chlorogenic acid
Crude extract, is detected under HPLC, is as a result shown, chlorogenic acid yield can reach 6.51% under the conditions of being somebody's turn to do.Reuse from
Sub- liquid 3 times, chlorogenic acid yield average is 6.23%.
6 ultrasounds of embodiment-method of pectinase chlorogenic acid extracting（1- ethyl-3-methylimidazole acetate）
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 0.5mol/L in ionic liquid 1- ethyl-3-methylimidazoles acetate, and solid-liquid ratio is 1:50, pectase
Consumption is 0.8%, and system pH is 4, and ultrasonic power is ultrasonic 40min under conditions of 80W.Then filter, filter residue is after extraction
Honeysuckle Flower, filtrate adds ethyl acetate in filtrate Jing after ethanol is reclaimed in vacuum distillation, locates respectively after system layering
Reason, removes layer reduced pressure concentration and reclaims ionic liquid, takes upper strata reduced pressure concentration and is chlorogenic acid crude extract, is filtered with 0.45 μm of micropore
After membrane filtration, detected under HPLC, as a result shown, under this condition, chlorogenic acid yield can be reached 6.42%.Repeat
Using ionic liquid 3 times, chlorogenic acid yield average is 6.39%.
7 ultrasounds of embodiment-method of pectinase chlorogenic acid extracting（1- butyl -3- ethyl imidazol(e) formates）
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 0.6mol/L in ionic liquid 1- butyl -3- ethyl imidazol(e)s formates, and solid-liquid ratio is 1:40, pectase
Consumption is 0.5%, and system pH is 4, and ultrasonic power is ultrasonic 40min under conditions of 90W.Then filter, filter residue is after extraction
Honeysuckle Flower, filtrate adds ethyl acetate in filtrate Jing after ethanol is reclaimed in vacuum distillation, locates respectively after system layering
Reason, removes layer reduced pressure concentration and reclaims ionic liquid, takes upper strata reduced pressure concentration and is chlorogenic acid crude extract, is filtered with 0.45 μm of micropore
Membrane filtration chlorogenic acid crude extract, is detected under HPLC, is as a result shown, under this condition chlorogenic acid yield can reach
6.50%.Reuse ionic liquid 3 times, chlorogenic acid yield average is 6.48%.
8 ultrasounds of embodiment-method of pectinase chlorogenic acid extracting（1- butyl -3- ethyl imidazol(e) villaumites）
After the size-reduced machine of honeysuckle is crushed, sieve, dry, precision weighs Honeysuckle Flower 2g, it is 70% to control concentration of alcohol, is surpassed
Sound temperature is 45 DEG C, is 0.9mol/L in ionic liquid 1- butyl -3- ethyl imidazol(e)s villaumite, and solid-liquid ratio is 1:30, pectase is used
Measure as 1.0%, system pH is 4, ultrasonic power is ultrasonic 30min under conditions of 70W.Then filter, filter residue is the gold after extracting
Honeysuckle flower powder, filtrate adds ethyl acetate in filtrate Jing after ethanol is reclaimed in vacuum distillation, processes respectively after system layering,
Remove layer reduced pressure concentration and reclaim ionic liquid, take upper strata reduced pressure concentration and be chlorogenic acid crude extract, with 0.45 μm of miillpore filter
Chlorogenic acid crude extract is filtered, is detected under HPLC, as a result shown, ortho acid recovery rate can reach 6.83% under the conditions of being somebody's turn to do.Weight
Again using ionic liquid 3 times, chlorogenic acid yield average is 6.82%.
Claims (9)
1. a kind of method of the chlorogenic acid extracting from honeysuckle, it is characterised in that the method is concretely comprised the following steps：
（1）Honeysuckle is smashed, in adding ionic liquid, mixture is uniformly mixed so as to obtain；
（2）Ethanol, 0.5-2% are added in the mixture（Honeysuckle dry weight）Enzyme, and adjust mixture pH value be 3-5；
（3）The ultrasonic system terminates to extraction；
（4）Filter and obtain filtrate；
（5）Concentration and recovery ethanol, and with ethyl acetate back extraction chlorogenic acid and ionic liquid is reclaimed, it is concentrated to give chlorogenic acid and slightly carries
Thing.
2. method according to claim 1, it is characterised in that the ionic liquid is methylimidazole villaumite, 1- butyl -3-
Ethyl imidazol(e) villaumite, 1- butyl -3- ethyl imidazol(e) formates, the one kind in 1- ethyl-3-methylimidazole acetate.In taking system
Concentration be 0.1-1.5mol/L.
3. method according to claim 1, it is characterised in that concentration of the ionic liquid in extraction system is 0.1-
1.5mol/L。
4. method according to claim 1, it is characterised in that described concentration of alcohol is 70%.
5. method according to claim 1, it is characterised in that the enzyme dosage accounts for the 0.5-2% of honeysuckle dry weight.
6. method according to claim 1, it is characterised in that described ultrasonic temperature is 45 DEG C, and ultrasonic time is 30-
60min, ultrasonic power is 60-100W.
7. method according to claim 1, it is characterised in that step（5）In, after first concentration and recovery ethanol, then by acetic acid
Ethyl ester is added in filtrate, treats to be separately recovered process after system layering, is removed layer reduced pressure concentration and is reclaimed ionic liquid, is taken upper strata and is subtracted
Pressure is concentrated to give chlorogenic acid crude extract.
8. method according to claim 1, it is characterised in that the enzyme is pectase or cellulase.
9. method according to claim 1, it is characterised in that the honeysuckle dry weight is 1 with ethanol volume ratio:30-50.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361204A (en) * | 2017-08-14 | 2017-11-21 | 胡玉涛 | The preparation method and animal feed of a kind of honeysuckle extract feed additive |
| CN108669533A (en) * | 2018-03-30 | 2018-10-19 | 杭州多源科技有限公司 | A kind of meal replacing food and preparation method thereof of conditioning shield stomach |
| CN108822305A (en) * | 2018-06-27 | 2018-11-16 | 西北农林科技大学 | A method of it is pre-processed based on glyoxaline ion liquid and extracts gutta-percha in eucommia bark |
| CN114028462A (en) * | 2021-11-26 | 2022-02-11 | 浙江宜格企业管理集团有限公司 | Method for extracting phenylpropanoid components from Prunus mume flower |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851163A (en) * | 2010-06-10 | 2010-10-06 | 桂阳济草堂药材种业有限公司 | Method for extracting high-purity chlorogenic acid from honey suckle flower |
| CN103086889A (en) * | 2013-02-22 | 2013-05-08 | 王星敏 | Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme |
| CN103435486A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing high-purity chlorogenic acid in honeysuckle |
| CN103524348A (en) * | 2013-09-29 | 2014-01-22 | 湖南金地生物科技有限公司 | Process for extracting chlorogenic acid from honeysuckle and dry leaves of honeysucklestem through biological enzyme |
| CN104628566A (en) * | 2013-11-09 | 2015-05-20 | 青岛中人智业生物科技有限公司 | Technology for extracting chlorogenic acid from honeysuckle flowers |
| CN106146309A (en) * | 2015-04-10 | 2016-11-23 | 浙江海洋学院 | A kind of method utilizing ionic liquid extract chlorogenic acid in Flos Chrysanthemi Indici |
-
2016
- 2016-12-15 CN CN201611162882.7A patent/CN106631799A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851163A (en) * | 2010-06-10 | 2010-10-06 | 桂阳济草堂药材种业有限公司 | Method for extracting high-purity chlorogenic acid from honey suckle flower |
| CN103086889A (en) * | 2013-02-22 | 2013-05-08 | 王星敏 | Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme |
| CN103435486A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing high-purity chlorogenic acid in honeysuckle |
| CN103524348A (en) * | 2013-09-29 | 2014-01-22 | 湖南金地生物科技有限公司 | Process for extracting chlorogenic acid from honeysuckle and dry leaves of honeysucklestem through biological enzyme |
| CN104628566A (en) * | 2013-11-09 | 2015-05-20 | 青岛中人智业生物科技有限公司 | Technology for extracting chlorogenic acid from honeysuckle flowers |
| CN106146309A (en) * | 2015-04-10 | 2016-11-23 | 浙江海洋学院 | A kind of method utilizing ionic liquid extract chlorogenic acid in Flos Chrysanthemi Indici |
Non-Patent Citations (3)
| Title |
|---|
| 居乃琥主编: "《酶工程手册》", 31 August 2011, 中国轻工业出版社 * |
| 李庆龙等: "离子液体微波辅助萃取金银花中绿原酸的研究", 《浙江海洋学院学报(自然科学版)》 * |
| 赵余庆等主编: "《食疗与保健食品原料功能因子手册》", 31 March 2013, 中国医药科技出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361204A (en) * | 2017-08-14 | 2017-11-21 | 胡玉涛 | The preparation method and animal feed of a kind of honeysuckle extract feed additive |
| CN108669533A (en) * | 2018-03-30 | 2018-10-19 | 杭州多源科技有限公司 | A kind of meal replacing food and preparation method thereof of conditioning shield stomach |
| CN108822305A (en) * | 2018-06-27 | 2018-11-16 | 西北农林科技大学 | A method of it is pre-processed based on glyoxaline ion liquid and extracts gutta-percha in eucommia bark |
| CN108822305B (en) * | 2018-06-27 | 2020-11-27 | 西北农林科技大学 | Method for extracting gutta-percha from eucommia bark based on imidazole ionic liquid pretreatment |
| CN114028462A (en) * | 2021-11-26 | 2022-02-11 | 浙江宜格企业管理集团有限公司 | Method for extracting phenylpropanoid components from Prunus mume flower |
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