CN106631127A - Geotechnical material of microbial granule and production method thereof - Google Patents
Geotechnical material of microbial granule and production method thereof Download PDFInfo
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- CN106631127A CN106631127A CN201611247699.7A CN201611247699A CN106631127A CN 106631127 A CN106631127 A CN 106631127A CN 201611247699 A CN201611247699 A CN 201611247699A CN 106631127 A CN106631127 A CN 106631127A
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- cementing
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- calcium chloride
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B38/00—Porous mortars, concrete, artificial stone or ceramic ware; Preparation thereof
- C04B38/08—Porous mortars, concrete, artificial stone or ceramic ware; Preparation thereof by adding porous substances
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B18/00—Use of agglomerated or waste materials or refuse as fillers for mortars, concrete or artificial stone; Treatment of agglomerated or waste materials or refuse, specially adapted to enhance their filling properties in mortars, concrete or artificial stone
- C04B18/04—Waste materials; Refuse
- C04B18/18—Waste materials; Refuse organic
- C04B18/20—Waste materials; Refuse organic from macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
- C04B2111/20—Resistance against chemical, physical or biological attack
- C04B2111/24—Sea water resistance
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2201/00—Mortars, concrete or artificial stone characterised by specific physical values
- C04B2201/50—Mortars, concrete or artificial stone characterised by specific physical values for the mechanical strength
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/91—Use of waste materials as fillers for mortars or concrete
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Ceramic Engineering (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Structural Engineering (AREA)
- Materials Engineering (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Environmental & Geological Engineering (AREA)
- Civil Engineering (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Curing Cements, Concrete, And Artificial Stone (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a geotechnical material of microbial granule and a production method thereof. The material comprises, by weight, 1-5 parts of foam plastic particles or fragments, 80-95 parts of calcareous soil and 5-9 parts of Pasteurella multocida fluid. The geotechnical material prepared by the method has the advantages of being light in mass, medium in strength, and good in stability. The geotechnical material of microbial granule can be produced by using discarded foam material and calcareous material, thus the cost is low. The geotechnical material can be used as the filler material for man-made islands of roadbed and pipe, and can also be used for preparing building materials such as light building blocks.
Description
Technical field
The present invention relates to Material Field, the earth working material and production method of the cementing foam beads of specifically a kind of microorganism.
Background technology
Foamed material is the great invention of the mankind, and widely, demand is very vigorous for its use range.But while it is also
A kind of material for being difficult to degrade, conventional processing method is generally filled and burned.
Landfill can take substantial amounts of soil, and burning would generally produce a large amount of toxic gases.They are all unfavorable for advocating now
The green economy led and sustainable development.Foamed material material is more frivolous on sea, often has fish dead because eating by mistake, but also meeting
Hinder the photosynthetic purifying water body of algae.Thus the harmless treatment of froth foams has a very big significance.
Coastal area is densely populated to need to build large-scale public civilian installation, and all kinds of municipal works, highway engineering,
Man-made island is built and is directed to digging up and filling in, and reasonable selection filling material becomes the problem that must take into design.
It is conventional banket mostly from riverbed sandstone, cut into a mountain native stone etc., these material sources are limited and in being normally at
Land area, transports very uneconomical.If filling material severe is larger, secondary problem such as end of the bridge may be brought to new construction to jump
Car, fill it is unreal etc..
With some strength again light weight and backfilling material simple and easy to get can in a large number save construction costs, produce preferable
Economic benefit.
Calcareous sand hole is more, and severe is gently distributed more widely in coastal area, but itself soft intensity it is relatively low should not be used as it is mixed
The aggregate of soil is coagulated, is easily crushed without cementing calcareous sand, intensity is not high to be also unsuitable for being used as filler.
Recent study find microorganism can inductive formation calcarinate calcareous sand, the reinforcing body intensity of formation is reachable
Several MPas so that calcareous sand is possibly realized for building field.
The content of the invention
Present invention aim to address in prior art obsolete material be difficult to recycle, construction material high cost, construction it is difficult
The problems such as；Present invention achieves two kinds discarded or useless material carries out resource recycling, propose with reference to biological cement a kind of
Can be used for construction material, the cementing foam beads earth working material of microorganism of roadbed filling and its production method.
The technical scheme adopted to realize the object of the invention is a kind of such, geotechnique of the cementing foam beads of microorganism
Material, it is characterised in that including following raw material and its parts by weight：
1～5 part of foam grain or fragment；
80～95 parts of calcareous sand；
5～9 parts of Bacillus pasteurii bacterium solution.
The production method of the earth working material of the cementing foam beads of a kind of microorganism, it is characterised in that comprise the following steps：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
The weight ratio of the foamed plastics, calcareous sand and Bath bacillus bacterium solution for (1～5) ︰ (80～95) ︰ (5～
9)；
2) mixed solution of calcium chloride and urea is added in mixture A；
The mol ratio of the calcium chloride and urea is the ︰ 2 of 1 ︰ 1～1；
Concentration of the calcium chloride in mixed solution is 55.94～110.98g/L；
Further, the particle size range of the foam grain or fragment is 0.5～3mm.
Further, the particle size range of the calcareous sand is 0.01～2mm.
Further, the concentration of Bacillus pasteurii is 10 in the Bacillus pasteurii bacterium solution7-109CFU/mL。
Further, the foamed plastics, calcareous sand, calcium chloride and urea are commercially available.
Further, also comprise the following steps including a kind of production method of the earth working material for preparing given shape：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
The weight ratio of the foamed plastics, calcareous sand and Bath bacillus bacterium solution for (1～5) ︰ (80～95) ︰ (5～
9)；
2) mixture A is poured into the mould of respective shapes；
3) in step 2) mould in add the mixed solution of calcium chloride and urea, the demoulding after 4～8 days；
The mol ratio of the calcium chloride and urea is the ︰ 2 of 1 ︰ 1～1；
Concentration of the calcium chloride in mixed solution is 55.94～110.98g/L.
The solution have the advantages that it is mathematical, the present invention have advantages below：
1) extensive usability：Earth working material severe in the present invention is light, and dry density is in 0.3～0.8g/cm3.As road
Conduct oneself with dignity when base, airfield runway filler it is little can reduce settlement after construction, be conducive to long-distance transportation.For man-made island filler, salt tolerant
Alkali is effective against the corrosion function of waves of seawater.Under identical quality, the volume of the earth working material is larger, is suitable for ore body mined out
The backfill in area.Foam has good heat insulating effect, by light-weight brick made by the present invention can also masonry panel reach and build
Build the purpose of energy-conservation.
2) changing waste into resources is utilized：Have in raw material two kinds be without value or discarded object, made by earth working material
It is relatively cheap, while having reached the purpose of twice laid.General lightweight soil consolidator and cement, microorganism is cementing will not to be produced
The calcium carbonate environmentally safe that carbon emission is generated.
3) good adaptability：The amount that the urea and calcium chloride mixed solution for adding can as needed be controlled adjusts cementing
Degree reach different strength and stiffness.Foam beads stability after cementing is good.
Description of the drawings
The cylinder sample schematic diagram that Fig. 1 is formed for the cementing foam beads light soil of obtained microorganism in embodiment 1；
The cylinder sample schematic diagram that Fig. 2 is formed for the cementing foam beads light soil of obtained microorganism in embodiment 2；
Fig. 3 is that the cementing foam beads light soil of microorganism is used for roadbed material schematic diagram；
Fig. 4 fills schematic diagram for the cementing foam beads light soil of microorganism for man-made island；
Fig. 5 is that the cementing foam beads light soil of microorganism is used for barricade backfill schematic diagram.
Specific embodiment
With reference to embodiment, the invention will be further described, but should not be construed above-mentioned subject area of the invention only
It is limited to following embodiments.Without departing from the idea case in the present invention described above, according to ordinary skill knowledge and used
With means, various replacements and change are made, all should be included within the scope of the present invention.
Embodiment 1：
The preparation of 1-1 Bacillus pasteurii bacterium solutions：
1) Bacillus pasteurii is seeded into culture medium, at 30 DEG C, culture 30～40 is little in 121r/min constant temperature oscillators
When.
Contain yeast extract 20g, manganese sulfate 25mg, nickel chloride 5mg, ammonium chloride 15g in every liter of culture medium, use hydroxide
PH is adjusted to 9-10 by sodium.
2) taking-up nutrient solution is concentrated in 4 DEG C of centrifuges and makes Bacillus pasteurii bacterium solution.
The concentration of Bacillus pasteurii is 10 in obtained Bacillus pasteurii bacterium solution8CFU/mL
The earth working material of the cementing foam beads of a kind of microorganisms of 1-2, it is characterised in that including following raw material and its weight portion
Number：
4.3 parts of foam grain or fragment；
87 parts of calcareous sand；
8.7 parts of Bacillus pasteurii bacterium solution.
The particle size range of the foam grain or fragment is 2-3mm.
The particle size range of the calcareous sand is 0.01-1mm.
1-3 is followed the steps below using load weighted raw material in 1-2：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
2) mixture A is poured into cylindrical backing cylinders；
3) mixed solution of calcium chloride and urea is added in cylindrical backing cylinder；
The mol ratio of the calcium chloride and urea is 1 ︰ 1；
0.5M calcium chloride and urea mixed solution are constantly passed through with the speed of 20mL/h, have often been led to 50mL and has been stood 3 hours, 6
The demoulding after it, prepares the earth working material of the cementing foam beads of cylindrical microorganism as shown in Figure 1.
By showing without lateral spacing experiment, the compression strength of the material prepared in the present embodiment is 110.7kPa, density
For 0.73g/cm3, can be used for the filler of roadbed.
Embodiment 2：
The preparation of 2-1 Bacillus pasteurii bacterium solutions：
1) Bacillus pasteurii is seeded into culture medium, at 30 DEG C, culture 30～40 is little in 121r/min constant temperature oscillators
When.
Contain yeast extract 20g, manganese sulfate 25mg, nickel chloride 5mg, ammonium chloride 15g in every liter of culture medium, use hydroxide
PH is adjusted to 9-10 by sodium.
2) taking-up nutrient solution is concentrated in 4 DEG C of centrifuges and makes Bacillus pasteurii bacterium solution.
The concentration of Bacillus pasteurii is 10 in obtained Bacillus pasteurii bacterium solution9CFU/mL
The earth working material of the cementing foam beads of a kind of microorganisms of 2-2, it is characterised in that including following raw material and its weight portion
Number：
1.3 parts of foam grain or fragment；
92.8 parts of calcareous sand；
6.9 parts of Bacillus pasteurii bacterium solution.
The particle size range of the foam grain or fragment is 0.5～1mm.
The particle size range of the calcareous sand is 0.01～2mm.
2-3 is followed the steps below using load weighted raw material in 1-2：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
2) mixture A is poured into cylindrical backing cylinders；
3) mixed solution of calcium chloride and urea is added in cylindrical backing cylinder；
The mol ratio of the calcium chloride and urea is 1 ︰ 1；
0.5M calcium chloride and urea mixed solution are constantly passed through with the speed of 20mL/h, have often been led to 50mL and has been stood 3 hours, 6
The demoulding after it, prepares the earth working material of the cementing foam beads of cylindrical microorganism as shown in Figure 2.
By showing without lateral spacing experiment, the compression strength of the material prepared in the present embodiment is 73.8kPa, and density is
0.49g/cm3。
The material that the present invention is prepared has extensive usability：
1) as shown in figure 3, be used as to conduct oneself with dignity when roadbed, airfield runway filler it is little can reduce settlement after construction, be conducive to it is long away from
From transport.
2) as shown in figure 4, being used for man-made island filler, Salt And Alkali Tolerance is effective against the corrosion function of waves of seawater.
3) as shown in figure 5, being used for barricade backfill, under identical quality, the volume of the earth working material is larger, is suitable for ore deposit
The backfill in body goaf.
Foam has good heat insulating effect, by light-weight brick made by the present invention can also masonry panel reach building
The purpose of energy-conservation.
Claims (7)
1. the earth working material of the cementing foam beads of a kind of microorganism, it is characterised in that including following raw material and its parts by weight：
1～5 part of foam grain or fragment；
80～95 parts of calcareous sand；
5～9 parts of Bacillus pasteurii bacterium solution.
2. the production method of the earth working material of the cementing foam beads of a kind of microorganism, it is characterised in that comprise the following steps：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
The weight ratio of the foamed plastics, calcareous sand and Bath bacillus bacterium solution is (1～5) ︰ (80～95) ︰ (5～9)；
2) mixed solution of calcium chloride and urea is added in mixture A；
The mol ratio of the calcium chloride and urea is the ︰ 2 of 1 ︰ 1～1；
Concentration of the calcium chloride in mixed solution is 55.94～110.98g/L.
3. the earth working material and production method of the cementing foam beads of a kind of microorganism according to claim 1 and 2, its feature
It is：The particle size range of the foam grain or fragment is 0.5～3mm.
4. the earth working material and production method of the cementing foam beads of a kind of microorganism according to claim 1 and 2, its feature
It is：The particle size range of the calcareous sand is 0.01～2mm.
5. the earth working material and production method of the cementing foam beads of a kind of microorganism according to claim 1 and 2, its feature
It is：The concentration of Bacillus pasteurii is 10 in the Bacillus pasteurii bacterium solution7-109CFU/mL。
6. a kind of production method of the earth working material of the cementing foam beads of microorganism according to claim 2, its feature exists
In：The foamed plastics, calcareous sand, calcium chloride and urea are commercially available.
7. a kind of production method of the earth working material of the cementing foam beads of microorganism according to claim 2, its feature exists
In：Also include a kind of production method of the earth working material for preparing given shape, comprise the following steps：
1) by foamed plastics, calcareous sand and Bath bacillus bacterium solution, it is stirred to being well mixed, obtains mixture A；
The weight ratio of the foamed plastics, calcareous sand and Bath bacillus bacterium solution is (1～5) ︰ (80～95) ︰ (5～9)；
2) mixture A is poured into the mould of respective shapes；
3) in step 2) mould in add the mixed solution of calcium chloride and urea, the demoulding after 4～8 days；
The mol ratio of the calcium chloride and urea is the ︰ 2 of 1 ︰ 1～1；
Concentration of the calcium chloride in mixed solution is 55.94～110.98g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611247699.7A CN106631127A (en) | 2016-12-29 | 2016-12-29 | Geotechnical material of microbial granule and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611247699.7A CN106631127A (en) | 2016-12-29 | 2016-12-29 | Geotechnical material of microbial granule and production method thereof |
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Family
ID=58836254
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| CN201611247699.7A Pending CN106631127A (en) | 2016-12-29 | 2016-12-29 | Geotechnical material of microbial granule and production method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107326870A (en) * | 2017-07-05 | 2017-11-07 | 海南大学 | A kind of processing method of Reef in Chinese South Sea hydraulic reclamation malm ground |
| CN109056311A (en) * | 2018-07-24 | 2018-12-21 | 郭跃 | A kind of preparation method of heat-insulated ablation-resistant glass fiber fabric |
| CN109485367A (en) * | 2019-01-07 | 2019-03-19 | 中南大学 | A kind of preparation method and application of filling phosphorous gypsum body |
| CN110863836A (en) * | 2019-10-15 | 2020-03-06 | 中南大学 | Assembled microorganism grouting shock-absorbing layer and preparation method and application thereof |
| CN111253129A (en) * | 2020-02-20 | 2020-06-09 | 於孝牛 | Preparation method of seawater calcareous sand basalt fiber cement-based composite material |
| CN113300311A (en) * | 2021-05-31 | 2021-08-24 | 中电保力（北京）科技有限公司 | Waterproof method for cable well or cable channel |
| CN116515701A (en) * | 2023-05-12 | 2023-08-01 | 龙建路桥股份有限公司 | Cold region roadbed foundation soil in-situ microorganism culture solution and expanding culture method and application thereof |
| CN117003542A (en) * | 2023-08-22 | 2023-11-07 | 江西理工大学 | Preparation method of bio-based polyurethane foam soil |
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|---|---|---|---|---|
| CN1448356A (en) * | 2003-05-07 | 2003-10-15 | 河海大学 | Formed plastic and particle mixed light-weight earth and production method thereof |
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| CN102718445A (en) * | 2012-06-25 | 2012-10-10 | 东南大学 | Tailing sand biological prefabricated product and preparation method thereof |
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2016
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448356A (en) * | 2003-05-07 | 2003-10-15 | 河海大学 | Formed plastic and particle mixed light-weight earth and production method thereof |
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| CN102718445A (en) * | 2012-06-25 | 2012-10-10 | 东南大学 | Tailing sand biological prefabricated product and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107326870A (en) * | 2017-07-05 | 2017-11-07 | 海南大学 | A kind of processing method of Reef in Chinese South Sea hydraulic reclamation malm ground |
| CN109056311A (en) * | 2018-07-24 | 2018-12-21 | 郭跃 | A kind of preparation method of heat-insulated ablation-resistant glass fiber fabric |
| CN109485367A (en) * | 2019-01-07 | 2019-03-19 | 中南大学 | A kind of preparation method and application of filling phosphorous gypsum body |
| CN110863836A (en) * | 2019-10-15 | 2020-03-06 | 中南大学 | Assembled microorganism grouting shock-absorbing layer and preparation method and application thereof |
| CN111253129A (en) * | 2020-02-20 | 2020-06-09 | 於孝牛 | Preparation method of seawater calcareous sand basalt fiber cement-based composite material |
| CN113300311A (en) * | 2021-05-31 | 2021-08-24 | 中电保力（北京）科技有限公司 | Waterproof method for cable well or cable channel |
| CN116515701A (en) * | 2023-05-12 | 2023-08-01 | 龙建路桥股份有限公司 | Cold region roadbed foundation soil in-situ microorganism culture solution and expanding culture method and application thereof |
| CN117003542A (en) * | 2023-08-22 | 2023-11-07 | 江西理工大学 | Preparation method of bio-based polyurethane foam soil |
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Application publication date: 20170510 |
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| RJ01 | Rejection of invention patent application after publication |