CN106622439A - Quantitative constant-temperature agar pouring device - Google Patents
Quantitative constant-temperature agar pouring device Download PDFInfo
- Publication number
- CN106622439A CN106622439A CN201611028584.9A CN201611028584A CN106622439A CN 106622439 A CN106622439 A CN 106622439A CN 201611028584 A CN201611028584 A CN 201611028584A CN 106622439 A CN106622439 A CN 106622439A
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- temperature
- agar
- constant
- plunger
- bottle
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L99/00—Subject matter not provided for in other groups of this subclass
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a quantitative constant-temperature agar pouring device. The quantitative constant-temperature agar pouring device is mainly applied to a biological or medical experiment. The quantitative constant-temperature agar pouring device comprises a liquid discharging switch, a quantifying valve, a plunger, a one-way valve, a sample feeding port, a bottle cover, an insulating layer, a storage bottle, a plunger cavity, a return spring, an inlet pipe, a fixing groove, a partition board, electric heating plates, a charging interface, a storage battery, a storage battery switch, a control circuit, a temperature sensor, a temperature display, a control cabinet, a sample feeding table, a constant-temperature water bath, a vent hole and an outlet pipe. The storage bottle with agar is put into the small charged constant-temperature water bath for thermal insulation, and during operation, the quantitative constant-temperature agar pouring device can be placed on a sterile working platform, and through adjustment of the quantifying valve below the liquid discharging switch, the agar pouring volume is controlled to quantitatively pour the constant-temperature agar so as to meet requirements of a pouring culture method, bacterium-containing agar plate preparation and other experiments on sterile operation as well as precise control over the agar pouring volume and the temperature. The quantitative constant-temperature agar pouring device is convenient to move, easy and safe to operate and low in cost, and has relatively high popularization and application values and very strong creativity.
Description
Technical field
The present invention relates to a kind of biology or medical experiment apparatus technical field, and in particular to a kind of quantitative constant-temperature agar inclines
Note device.
Background technology
Biology and many experiments of medical science need for 45 DEG C of constant temperature sterile letheen to be poured into addition containing germy sample
Mix in the culture dish of solution, or be poured into again in culture dish after bacterial suspension is first mixed with 45 DEG C of constant temperature sterile letheens.Fine jade
The temperature of fat is too high can be too low by solidification by the boiling hot dead, temperature of bacterium, generally agar need to be carried out into 45 DEG C of constant temperature insulations.In addition, being
Ensureing the repeatability of experiment need to quantitatively pour into agar, and agar to pour into needs complete (such as superclean bench) in gnotobasis
Into.Therefore, the stability of agar temperature, the accuracy of agar volume, agar pour into the direct shadow of three factors of aseptic of environment
Ring the success or failure of experiment.But so far satisfactory, the special agar of still ununified energy pours into device.
The method of at present agar constant temperature insulation is：The flask for holding agar is placed in the water-bath for setting temperature, is needed
When wanting the agar of good heat insulation taken out from water-bath again, be then poured in culture dish in aseptic working platform.Due to water-bath
Pot product is larger, and needs connection socket to power, and agar must generally be taken out and is placed in again in superclean bench by mobile more inconvenience
Pour into, thus 45 DEG C of agar also need to place a period of time at ambient temperature from after insulating box taking-up, easily cause agar because of temperature
Degree is reduced and solidified, and if agar temperature is improved with additive method bacterial death in agar is easily caused, so as to affect experiment knot
Really.Accordingly, it is capable to no in aseptic working platform be directly poured into constant temperature agar in culture dish is particularly important.
During agar is poured into culture dish, existing method is typically placed on the agar in conical flask according to Jing
Test and pour into into culture dish, this method is it is difficult to ensure that pour into every time identical to the agar amount of culture dish.If with pipettor or pipette
Waste time and energy Deng pouring into agar again and then operating after quantitative absorption, and easily solidify because agar is cooled down in operation.Therefore,
Can fast quantification to pour into constant temperature agar be also problem demanding prompt solution.
In sum, research fast quantification constant temperature can pour into the device of agar and have important application in aseptic working platform
Value.
The content of the invention
(1) technical problem for solving
For the problems referred to above, water-bath insulation and the manual empirical method for pouring into agar are taken in present invention change, propose
A kind of quantitative constant-temperature agar pours into device；Originally pour into device to be easy to move and integrate storage, insulation and quantitatively pour into function.
(2) technical scheme
To realize object above, the present invention is achieved by the following technical programs：
A kind of quantitative constant-temperature agar pours into device, including：Go out liquid switch, plunger, check valve, adding mouth, sample-adding platform, bottle cap,
Holding bottle, plunger cavity, passage, outlet, retracting spring, inlet tube, fixing groove, dividing plate, charging inlet, battery, electric power storage
Pond switch, control circuit, heat-insulation layer, proportional valve, temperature sensor, temperature indicator, control cabinet, Water Tank with Temp.-controlled and electric hot plate；
Dividing plate is provided with the Water Tank with Temp.-controlled, dividing plate is provided with through hole and fixing groove；Temperature sensor is provided with the Water Tank with Temp.-controlled, temperature
Temperature in Water Tank with Temp.-controlled is fed back to degree sensor the control circuit in control cabinet, and control circuit is by contrast design temperature and biography
Sensor feedback temperature is deciding whether to be powered to electric hot plate so that Water Tank with Temp.-controlled is in constant temperature, with meet pour plate method and
Agar temperature is precisely controlled in the experiments such as the preparation of flat board containing bacterio-agar, and pours into device and be provided with the temperature being connected with temperature sensor
Degree display, can temperature in display channel in real time.
Preferably, the plunger cavity top is provided with passage, passage film containing bacteriological filtration, it is ensured that air pressure and extraneous gas in bottle
Flatten weighing apparatus and the aseptic gas Jing after EK are into the gas in bottle.
Preferably, the dividing plate can avoid holding bottle and electric hot plate directly contact, so as to prevent agar moment temperature in bottle
Height is spent, the water above and below the dividing plate can be by through hole convection current, so as to accelerate the heating to agar, fixing groove and storage
Bottle forms snap-in structure, can prevent moving horizontally and because of moving up and down that buoyancy causes, the dividing plate and fixing groove for holding bottle
The security in heating process is can ensure that, through hole can ensure the stability of heating process.
Preferably, the bottle cap lower section connection plunger cavity, is provided with retracting spring in plunger cavity, and retracting spring top is post
Plug, plunger can be moved in plunger cavity and air-tightness is good, and plunger is provided with scale, and proportional valve can be moved on plunger, will be fixed
Bolt is tightened and can fix its position on amount valve, can quantitatively control to pour into agar every time by adjusting out the proportional valve below liquid switch
Volume, with meeting pour plate method and flat board containing bacterio-agar and preparing etc. agar volume is precisely controlled in experiment, and quantitatively
Valve is simple and quick.
Preferably, the outlet, plunger, plunger cavity, bottle cap, inlet tube are detachably and by high pressure resistant steam sterilizing material
Material is made, and facilitates its cleaning and sterilizes.
Preferably, control cabinet top is provided with sample-adding platform, make agar export from culture dish closer to and sample-adding platform is also protected
Hold constant temperature.The culture dish for needing to add agar is positioned on sample-adding platform, can avoid being made because the table top of culture dish holding is subcooled
The rapid cooled and solidified of agar, while it also avoid the appearance of the phenomenons such as agar drippage or pollution test stand.
Preferably, rechargeable type battery, the rechargeable type battery and the battery are provided with the control cabinet
Switch be connected, for the power supply of control cabinet, which obviate because socket need to be connected it is caused using limitation, and pour into body product compared with
Little, conveniently moving can be independently positioned over used in aseptic working platform.
Preferably, the holding bottle is wide-mouth bottle, and easy to clean, bottle cap is provided with the adding mouth with tegillum, can pass through
Adding mouth is loaded, and the trouble for turning on storage bottle cap is eliminated, while decreasing the pollution that screw capping is caused.
Preferably, described quantitative constant-temperature agar pours into device and can be used to quantitatively pour into constant temperature agar or appoint for quantitatively pouring into
What his isothermal liquid.
(3) beneficial effect
Relative to the insulation of existing agar and dump method, beneficial effects of the present invention are embodied in：
First, the present invention can store agar and be at temperature constant state, and due to being provided with battery, be movable to aseptic work
Where making required for any experiment such as platform, so as to directly pour into into culture dish, the container because agar need to be will be equipped with is solved
It is transferred to from attemperator and pours into agar in the aseptic operating platform of room temperature and caused agar solidifies because of temperature drop
Puzzlement, improve conventional efficient.
2nd, the present invention is provided with proportional valve, can quantitatively pour into the agar of temperature constant state into culture dish, so as to ensure same group
Agar amount is identical in each culture dish in test, makes experimental result more accurate, and simple and quick, improves the behaviour of experiment
Make speed and accuracy.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the connection control figure of the present invention；
Label in figure is represented respectively：
1st, liquid switch is gone out；2nd, proportional valve；3rd, plunger；4th, check valve；5th, adding mouth；6th, bottle cap；7th, heat-insulation layer；8th, store
Bottle；9th, plunger cavity；10th, retracting spring；11st, check valve；12nd, inlet tube；13rd, fixing groove；14th, dividing plate；15th, electric hot plate；16th, fill
Electrical interface；17th, battery；18th, battery switch；19th, control circuit；20th, temperature sensor；21st, temperature indicator；22nd, control
Case processed；23rd, it is loaded platform；24th, Water Tank with Temp.-controlled；25th, passage；26th, outlet.
Specific embodiment
To make purpose, technical scheme and the advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention, rather than the embodiment of whole.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Referring to Fig. 1, a kind of quantitative constant-temperature agar pours into device, including：Go out liquid switch, proportional valve, plunger, check valve, sample-adding
Mouth, bottle cap, heat-insulation layer, holding bottle, plunger cavity, retracting spring, inlet tube, fixing groove, dividing plate, electric hot plate, charging inlet, electric power storage
Pond, battery switch, control circuit, temperature sensor, temperature indicator, control cabinet, sample-adding platform, Water Tank with Temp.-controlled, passage, go out
Mouth pipe.The device left side is poured into for Water Tank with Temp.-controlled 24, the right is control cabinet 22, the outer layer of Water Tank with Temp.-controlled 24 is one layer of heat preservation layer 7, thermostatted water
The bottom of groove 24 is provided with electric hot plate 15, and to be provided with fixing groove 13 and the dividing plate with through hole 14, holding bottle 8 can lead to the top of electric hot plate 15
Cross fixing groove 13 to be fixed on dividing plate 14, can contain suitable quantity of water and surround the bottle of holding bottle 8 in Water Tank with Temp.-controlled 24.In holding bottle 8
Inlet tube 12, plunger cavity 9, plunger 3 are from bottom to top followed successively by, plunger 3 connects liquid switch 1 and outlet 26, the bottom of plunger cavity 9
Be equipped with direction check valve 4 upwards inside plunger 3, the top of plunger 3 is provided with proportional valve 2 and indicates scale.When slow pressing
When going out liquid switch 1, plunger 3 is moved from top to bottom in plunger cavity 9, and retracting spring 10 is compressed, and now check valve 11 is closed, single
Open to valve 4, agar is pressed out in the culture dish being placed on sample-adding platform 23, when proportional valve 2 drops to bottle cap in plunger cavity 9
At 6, direction switch 1 stops declining, and the agar volume of extrusion is the scale amount pointed by proportional valve 2.After hand unclamps, quilt
The retracting spring 10 of compression up carries out plunger 3, and now check valve 4 is closed, and check valve 11 is opened, and agar is by the quilt of holding bottle 8
It is drawn in plunger cavity 9.The top of plunger cavity 9 is provided with passage 25, it is ensured that air pressure is flattened with air in holding bottle 8 during this
Weighing apparatus.Repeat this process, you can successively quantitatively pour into agar to culture dish.
Referring to Fig. 2, battery switch 18 is opened, battery is powered by control circuit 19 to electric hot plate 15, TEMP
Device 20 is determined in Water Tank with Temp.-controlled 24 and temperature and transmits signals to control circuit 19.When temperature is warm less than setting in Water Tank with Temp.-controlled 24
Degree, then control circuit 19 continues to be powered to electric hot plate 15, when temperature reaches design temperature, then control circuit 19 in Water Tank with Temp.-controlled 24
Interrupt to the power supply of electric hot plate 15, so that temperature keeps constant in Water Tank with Temp.-controlled 24.Temperature indicator 21 can in real time show perseverance
Temperature in warm water tank 24, when the temperature stabilization of Water Tank with Temp.-controlled 24 is to design temperature, you can carry out agar and pour into work.Experiment is completed
Afterwards, battery switch 18 is closed.
Relative to the insulation of existing agar and dump method, beneficial effects of the present invention are embodied in：
First, the present invention can store agar and be at temperature constant state, and due to being provided with battery, be movable to aseptic work
Where making required for any experiment such as platform, so as to directly pour into into culture dish, the container because agar need to be will be equipped with is solved
It is transferred to from attemperator and pours into agar in the aseptic operating platform of room temperature and caused agar solidifies because of temperature drop
Puzzlement, improve conventional efficient.
2nd, the present invention is provided with proportional valve, can quantitatively pour into the agar of temperature constant state into culture dish, so as to ensure same group
Agar amount is identical in each culture dish in test, makes experimental result more accurate, and simple and quick, improves the behaviour of experiment
Make speed and accuracy.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that a series of process, method, article or equipment including key elements not only will including those
Element, but also including other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
Above example only to illustrate technical scheme, rather than a limitation；Although with reference to the foregoing embodiments
The present invention has been described in detail, it will be understood by those within the art that：It still can be to aforementioned each enforcement
Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic；And these modification or
Replace, do not make the spirit and scope of the essence disengaging various embodiments of the present invention technical scheme of appropriate technical solution.
Claims (9)
1. a kind of quantitative constant-temperature agar pours into device, including：Go out liquid switch, plunger, check valve, adding mouth, sample-adding platform, bottle cap, deposit
Storage bottle, plunger cavity, passage, outlet, retracting spring, inlet tube, fixing groove and dividing plate, it is characterised in that：Including charging
Mouth, battery, battery switch, control circuit, heat-insulation layer, proportional valve, temperature sensor, temperature indicator, control cabinet, constant temperature
Tank and electric hot plate；Dividing plate is provided with the Water Tank with Temp.-controlled, dividing plate is provided with through hole and fixing groove；It is provided with the Water Tank with Temp.-controlled
Temperature in Water Tank with Temp.-controlled is fed back to temperature sensor, temperature sensor the control circuit in control cabinet, and control circuit is by right
Decide whether to be powered to electric hot plate with sensor feedback temperature than design temperature, so that Water Tank with Temp.-controlled is in constant temperature, with full
Agar temperature is precisely controlled in the experiment such as sufficient pour plate method and the preparation of flat board containing bacterio-agar, and pours into device and be provided with and temperature
The temperature indicator of sensor connection, can temperature in display channel in real time.
2. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The plunger cavity top is provided with ventilation
Hole, passage film containing bacteriological filtration, it is ensured that air pressure is balanced with ambient pressure and is Jing EKs into the gas in bottle in bottle
Aseptic gas afterwards.
3. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The dividing plate can avoid holding bottle
With electric hot plate directly contact, so as to prevent agar instantaneous temperature in bottle too high, the water above and below the dividing plate can be by logical
Hole convection current, so as to accelerate the heating to agar, fixing groove and holding bottle to form snap-in structure, moving horizontally for holding bottle can be prevented
With moving up and down for causing because of buoyancy, the dividing plate and fixing groove can ensure that the security in heating process, and through hole can ensure
The stability of heating process.
4. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The bottle cap lower section connection plunger
Chamber, is provided with retracting spring in plunger cavity, retracting spring top is plunger, and plunger can be moved in plunger cavity and air-tightness is good,
Plunger is provided with scale, and proportional valve can be moved on plunger, and bolt on proportional valve is tightened can fix its position, by adjusting out
Proportional valve below liquid switch can quantitatively control to pour into the volume of agar every time, to meet pour plate method and flat board containing bacterio-agar
Agar volume is precisely controlled in the experiment such as preparation, and proportional valve is simple and quick.
5. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The outlet, plunger, plunger
Chamber, bottle cap, inlet tube are detachable and are made up of high pressure resistant steam sterilizing material, facilitate its cleaning and sterilize.
6. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The control cabinet top is provided with sample-adding
Platform, make agar export from culture dish closer to and make sample-adding platform also keep constant temperature.Will need add agar culture dish be positioned over plus
On sample platform, can avoid making the rapid cooled and solidified of agar because the table top of culture dish holding is subcooled, while it also avoid agar drippage
Or the appearance of the phenomenon such as pollution test stand.
7. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：It is provided with chargeable in the control cabinet
Formula battery, the rechargeable type battery is connected with the battery switch, for the power supply of control cabinet, which obviates because needing
Connect socket and caused use limitation, and pour into device small volume, conveniently moving, independently can be positioned in aseptic working platform makes
With.
8. quantitative constant-temperature agar according to claim 1 pours into device, it is characterised in that：The holding bottle is wide-mouth bottle, clearly
Wash conveniently, bottle cap is provided with the adding mouth with tegillum, can be loaded by adding mouth, eliminate and turn on storage bottle cap
Trouble, while decreasing the pollution that screw capping is caused.
9. the quantitative constant-temperature agar according to claim 1-8 pours into the using method of device, and described quantitative constant-temperature agar inclines
Note device can be used to quantitatively pour into constant temperature agar or for quantitatively pouring into any other isothermal liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611028584.9A CN106622439B (en) | 2016-11-18 | 2016-11-18 | A kind of quantitative constant-temperature agar pours into device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611028584.9A CN106622439B (en) | 2016-11-18 | 2016-11-18 | A kind of quantitative constant-temperature agar pours into device |
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| Publication Number | Publication Date |
|---|---|
| CN106622439A true CN106622439A (en) | 2017-05-10 |
| CN106622439B CN106622439B (en) | 2018-09-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611028584.9A Active CN106622439B (en) | 2016-11-18 | 2016-11-18 | A kind of quantitative constant-temperature agar pours into device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107617450A (en) * | 2017-09-06 | 2018-01-23 | 张健 | Disposable quantitative liquid feeding group cup |
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| CN203470018U (en) * | 2013-09-10 | 2014-03-12 | 浙江农林大学 | Device for metering amount of added liquid through nitrogen filling and oxygen dispensing |
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| CN204685126U (en) * | 2015-06-09 | 2015-10-07 | 孟如苗 | A kind of efficient constant-temperature water-bath |
| CN105857933A (en) * | 2016-05-16 | 2016-08-17 | 中国人民解放军总医院第附属医院 | Medicine storage device |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2937068A1 (en) * | 1979-09-13 | 1981-04-02 | Fa. Johannes Bockemühl, 5270 Gummersbach | Tempering unit with retaining grip for bottles - storing photographic film developing fluids at set temperatures |
| DE3327688A1 (en) * | 1983-08-01 | 1985-02-21 | Friedrich 4790 Paderborn Fafflok | Container for storing and/or transporting sensitive products |
| CN1187449A (en) * | 1997-01-06 | 1998-07-15 | 黄添财 | Simple liquid drawing device |
| CN203187662U (en) * | 2013-01-30 | 2013-09-11 | 广西师范大学 | Lab culture medium preparing and split charging device |
| CN203470018U (en) * | 2013-09-10 | 2014-03-12 | 浙江农林大学 | Device for metering amount of added liquid through nitrogen filling and oxygen dispensing |
| CN204503137U (en) * | 2015-01-30 | 2015-07-29 | 河南健康源生物科技有限公司 | A kind of thermostat water bath |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107617450A (en) * | 2017-09-06 | 2018-01-23 | 张健 | Disposable quantitative liquid feeding group cup |
| CN107617450B (en) * | 2017-09-06 | 2019-06-21 | 张健 | Disposable quantitative liquid feeding group cup |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106622439B (en) | 2018-09-18 |
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Application publication date: 20170510 Assignee: Hefei Ronghong Technology Co.,Ltd. Assignor: Anhui University of Science and Technology Contract record no.: X2023980032327 Denomination of invention: A quantitative thermostatic agar pouring device Granted publication date: 20180918 License type: Common License Record date: 20230220 |