CN106620883A - Tissue-engineering three-dimensional porous bracket and manufacturing method thereof - Google Patents
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Abstract
The invention provides a tissue-engineering three-dimensional porous bracket and a manufacturing method thereof. The manufacturing method comprises the following steps: (1) adding modified starch, sodium hyaluronate, collagen protein, soybean protein isolate, whey protein and vital gluten into twice weight of water which is heated to 45-90 DEG C, and sufficiently stirring for 0.5-3 hours to obtain a mixed solution; (2) keeping the temperature of the mixed solution to be 30-90 DEG C, adding citric acid and acetic anhydride, and stirring for 1-4 hours; (3) continuously adding superfine red clay, feldspar powder, nano-hydroxyapatite, coral reef and mannitol, and continuously stirring; (4) weighing a solution obtained in the step (3), pouring the solution into a container, putting the container into a freezing chamber with the temperature of -80 DEG C to -20 DEG C to freeze for 12-48 hours, and transferring the container into a freeze drier to perform freeze-drying for 6-36 hours, thereby obtaining the tissue-engineering three-dimensional porous bracket. The three-dimensional porous bracket provided by the invention has very good substance exchange inside and outside the bracket, and has very good biomechanical property.
Description
Technical field
The present invention relates to field of medical materials, and in particular to a kind of organizational project three-dimensional porous rack and preparation method thereof.
Background technology
Organizational project is, using zoobiotism and engineering principles and method, to go the normal and diseased tissue for recognizing mammal
Structure-function relationship, to study, be developed for repair and safeguard or promote the various tissues of human body or the function after organ injury
With a new disciplines of form.The twentieth century organizational project eighties just causes worldwide concern once proposition.Through
The research of more than ten years, organizational project is developed rapidly.Timbering material for organizational project is good except having
Outside the biocompatible properties such as biocompatibility, cellular affinity, degradability, it is necessary to meet certain mechanical property and physics
Performance, such as：Suitable aperture, porosity, higher specific surface area etc..Say from the angle of cell culture, support only in this way
Just can play a supportive role, and the transport of favourable nutriment and metabolite is exchanged, and to cell good growing environment is provided.Although porous
The exploration from the initial stage of the technology of preparing of support has many performance requirements not reach gradually forward to clinical practice stage development
Arrive, such as：High porosity and it is capable of the three dimensional structure of internal UNICOM, such structure can be exchanged and generation for cell growth, nutrient
The circulation for thanking to product provides enough spaces；There are enough mechanical performances again simultaneously, meet the stress demand in internal milieu.
The content of the invention
The technical problem to be solved：It is an object of the invention to provide a kind of organizational project three-dimensional porous rack, porosity height,
Hole connectedness is good, and mechanical property is preferable, with mass exchange and biomechanical property inside and outside good support.
Technical scheme：A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：Modified starch
10-20 parts, hyaluronate sodium 15-35 parts, bone collagen 2-15 parts, nanometer hydroxyapatite 60-75 parts, coral reef 5-15
Part, citric acid 1.5-3 parts, acetic anhydride 1.8-3.6 parts, Mannitol 4.5-16.5 part, soybean protein isolate 12-30 parts, milk surum egg
White 10-25 parts, gluten meal 5-10 parts, ultra-fine red clay 2-8 parts, feldspar powder 2-8 parts.
It is further preferred that a kind of described organizational project three-dimensional porous rack, prepared by weight by following component and
Into：Modified starch 13-18 parts, hyaluronate sodium 20-30 parts, bone collagen 5-12 parts, nanometer hydroxyapatite 64-70 parts,
Coral reef 8-12 parts, citric acid 2-2.6 parts, acetic anhydride 2.5-3 parts, Mannitol 8-14 part, soybean protein isolate 18-25 parts, breast
Albumin 15-20 parts, gluten meal 6-9 parts, ultra-fine red clay 3-7 parts, feldspar powder 4-6 parts.
The preparation method of above-mentioned organizational project three-dimensional porous rack, comprises the following steps：
（1）By modified starch, hyaluronate sodium, bone collagen, soybean protein isolate, lactalbumin, gluten meal, 2 times are added
Weight portion, and be heated in 45-90 DEG C of water, 0.5-3h is sufficiently stirred for, obtain mixed solution；
（2）It is 30-90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1-4h；
（3）Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are continuously added, continues to stir；
（4）Weigh step（3）Solution is obtained, in pouring into container, freezing 12-48 hours in -20 DEG C of refrigerating chambers of -80- is subsequently placed in
Afterwards, then lyophilization 6-36 hours in freezer dryer are moved into, obtains final product organizational project three-dimensional porous rack.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（1）Middle water temperature is
60-80 DEG C, mixing time is 1-2h.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（2）Middle temperature is
45-75 DEG C, mixing time is 3h.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（3）In it is ultra-fine glutinous
The fineness of soil and feldspar powder is 200-300 mesh.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（4）Middle refrigerating chamber
Temperature be -30 DEG C of -65-, cooling time is 24-48h, the cryodesiccated time be 12-24h.
Beneficial effect：The porosity of the organizational project three-dimensional porous rack of the present invention has good between 85.5 to 92.1%
Hole connectivity；Comprcssive strength is can ensure that on the basis of high porosity is kept for 2.49-3.12MPa, it is ultra-fine adding
After red clay, feldspar powder, the mechanical property of porous support has significant raising.
Specific embodiment
Embodiment 1
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：20 parts of modified starch, hyaluronic acid
It is 35 parts of sodium, 2 parts of bone collagen, 60 parts of nanometer hydroxyapatite, 15 parts of coral reef, 1.5 parts of citric acid, 1.8 parts of acetic anhydride, sweet
4.5 parts of alcohol of dew, 12 parts of soybean protein isolate, 25 parts of lactalbumin, 5 parts of gluten meal, 2 parts of ultra-fine red clay, 2 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 45 DEG C of water, are sufficiently stirred for
3h, obtains mixed solution；（2）It is 30 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 4h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 48 hours in -20 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 6 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 2
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：10 parts of modified starch, hyaluronic acid
15 parts of sodium, 15 parts of bone collagen, 75 parts of nanometer hydroxyapatite, 5 parts of coral reef, 3 parts of citric acid, 3.6 parts of acetic anhydride, manna
16.5 parts of alcohol, 30 parts of soybean protein isolate, 10 parts of lactalbumin, 10 parts of gluten meal, 8 parts of ultra-fine red clay, 8 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 90 DEG C of water, are sufficiently stirred for
0.5h, obtains mixed solution；（2）It is 90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1h；（3）After
It is continuous to add ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）
Solution is obtained, in pouring into container, is subsequently placed in after freezing 12 hours in -80 DEG C of refrigerating chambers, then move into freezing in freezer dryer
It is dried 36 hours, obtains final product organizational project three-dimensional porous rack.
Embodiment 3
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：18 parts of modified starch, hyaluronic acid
20 parts of sodium, 5 parts of bone collagen, 64 parts of nanometer hydroxyapatite, 12 parts of coral reef, 2 parts of citric acid, 2.5 parts of acetic anhydride, manna
8 parts of alcohol, 25 parts of soybean protein isolate, 15 parts of lactalbumin, 6 parts of gluten meal, 3 parts of ultra-fine red clay, 6 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 60 DEG C of water, are sufficiently stirred for
2h, obtains mixed solution；（2）It is 45 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 24 hours in -65 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 12 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 4
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：13 parts of modified starch, hyaluronic acid
30 parts of sodium, 12 parts of bone collagen, 70 parts of nanometer hydroxyapatite, 8 parts of coral reef, 2.6 parts of citric acid, 3 parts of acetic anhydride, manna
14 parts of alcohol, 18 parts of soybean protein isolate, 20 parts of lactalbumin, 9 parts of gluten meal, 7 parts of ultra-fine red clay, 4 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 80 DEG C of water, are sufficiently stirred for
1h, obtains mixed solution；（2）It is 75 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 48 hours in -30 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 24 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 5
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：15 parts of modified starch, hyaluronic acid
It is 25 parts of sodium, 9 parts of bone collagen, 68 parts of nanometer hydroxyapatite, 10 parts of coral reef, 2.4 parts of citric acid, 2.7 parts of acetic anhydride, sweet
12 parts of alcohol of dew, 22 parts of soybean protein isolate, 18 parts of lactalbumin, 8 parts of gluten meal, 5 parts of ultra-fine red clay, 5 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 70 DEG C of water, are sufficiently stirred for
1.5h, obtains mixed solution；（2）It is 60 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）After
It is continuous to add ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）
Solution is obtained, in pouring into container, is subsequently placed in after freezing 36 hours in -40 DEG C of refrigerating chambers, then move into freezing in freezer dryer
It is dried 18 hours, obtains final product organizational project three-dimensional porous rack.
Comparative example 1
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：20 parts of modified starch, hyaluronic acid
It is 35 parts of sodium, 2 parts of bone collagen, 60 parts of nanometer hydroxyapatite, 15 parts of coral reef, 1.5 parts of citric acid, 1.8 parts of acetic anhydride, sweet
4.5 parts of alcohol of dew, 2 parts of ultra-fine red clay, 2 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, adds 2 times of weight portions, and is heated in 45 DEG C of water, is sufficiently stirred for 3h, obtains mixed solution；（2）Mixed solution is protected
Temperature is held for 30 DEG C, citric acid, acetic anhydride is added, 4h is stirred；（3）Continuously add ultra-fine red clay, feldspar powder, nano hydroxyapatite
Lime stone, coral reef, Mannitol, continue to stir；（4）Weigh step（3）Obtain solution, in pouring into container, be subsequently placed in -20 DEG C it is cold
Freeze after freezing 48 hours in room, then move into lyophilization 6 hours in freezer dryer, obtain final product organizational project three-dimensional porous rack.
Comparative example 2
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：10 parts of modified starch, hyaluronic acid
15 parts of sodium, 15 parts of bone collagen, 75 parts of nanometer hydroxyapatite, 5 parts of coral reef, 3 parts of citric acid, 3.6 parts of acetic anhydride, manna
16.5 parts of alcohol, 30 parts of soybean protein isolate, 10 parts of lactalbumin, 10 parts of gluten meal.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 90 DEG C of water, are sufficiently stirred for
0.5h, obtains mixed solution；（2）It is 90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1h；（3）After
It is continuous to add nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）Solution is obtained, container is poured into
In, it is subsequently placed in after freezing 12 hours in -80 DEG C of refrigerating chambers, then lyophilization 36 hours in freezer dryer are moved into, obtain final product group
Weaver's journey three-dimensional porous rack.
The method of testing of porosity：The porosity of porous support is measured with liquid substitution method.It is V first to measure its volume,
W is weighed as, ultrasound 5min in dehydrated alcohol is dipped in, is then placed under vacuum environment, negative pressure fully penetrates into dehydrated alcohol
Timbering material, taking-up is weighed as W1, and dehydrated alcohol density is designated as d, then the porosity of porous material：P=（W1-W）/（V·d）,
5 parallel tests are done, its meansigma methods is sought.
Mechanical measuring and calculation method：With Japanese Shimadzu material mechanical test machine（AGIC 50KN）Before and after determining porous material modification
Comprcssive strength, sample for 5mm × 5mm × 10mm cube, test speed is 0.5mm/min, and compression height ratio is 60%,
Every group of sample does 5 parallel laboratory tests.
The property indices of material of the present invention see the table below, it may be seen that the hole of this organizational project three-dimensional porous rack
Gap rate has good hole connectivity between 85.5 to 92.1%；Pressure resistance is can ensure that on the basis of high porosity is kept
Spend for 2.49-3.12MPa, after ultra-fine red clay, feldspar powder is added, the mechanical property of porous support has significant raising.
The property indices of the organizational project three-dimensional porous rack of table 1
| Name of product | Porosity（%） | Comprcssive strength（MPa） |
| Embodiment 1 | 85.5 | 2.49 |
| Embodiment 2 | 87.2 | 2.55 |
| Embodiment 3 | 88.5 | 2.65 |
| Embodiment 4 | 89.6 | 2.87 |
| Embodiment 5 | 92.1 | 3.12 |
| Comparative example 1 | 70.5 | 2.50 |
| Comparative example 2 | 86.1 | 1.41 |
Claims (7)
1. a kind of organizational project three-dimensional porous rack, it is characterised in that be prepared from by weight by following component：Modified starch
10-20 parts, hyaluronate sodium 15-35 parts, bone collagen 2-15 parts, nanometer hydroxyapatite 60-75 parts, coral reef 5-15
Part, citric acid 1.5-3 parts, acetic anhydride 1.8-3.6 parts, Mannitol 4.5-16.5 part, soybean protein isolate 12-30 parts, milk surum egg
White 10-25 parts, gluten meal 5-10 parts, ultra-fine red clay 2-8 parts, feldspar powder 2-8 parts.
2. a kind of organizational project three-dimensional porous rack according to claim 1, it is characterised in that by following component by weight
Part is prepared from：Modified starch 13-18 parts, hyaluronate sodium 20-30 parts, bone collagen 5-12 parts, nanometer hydroxyapatite
64-70 parts, coral reef 8-12 parts, citric acid 2-2.6 parts, acetic anhydride 2.5-3 parts, Mannitol 8-14 part, soybean protein isolate 18-
25 parts, lactalbumin 15-20 parts, gluten meal 6-9 parts, ultra-fine red clay 3-7 parts, feldspar powder 4-6 parts.
3. a kind of preparation method of organizational project three-dimensional porous rack, it is characterised in that comprise the following steps：
（1）By modified starch, hyaluronate sodium, bone collagen, soybean protein isolate, lactalbumin, gluten meal, 2 times are added
Weight portion, and be heated in 45-90 DEG C of water, 0.5-3h is sufficiently stirred for, obtain mixed solution；
（2）It is 30-90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1-4h；
（3）Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are continuously added, continues to stir；
（4）Weigh step（3）Solution is obtained, in pouring into container, freezing 12-48 hours in -20 DEG C of refrigerating chambers of -80- is subsequently placed in
Afterwards, then lyophilization 6-36 hours in freezer dryer are moved into, obtains final product organizational project three-dimensional porous rack.
4. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（1）Middle water temperature is 60-80 DEG C, and mixing time is 1-2h.
5. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（2）Middle temperature is 45-75 DEG C, and mixing time is 3h.
6. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（3）In ultra-fine clay and feldspar powder fineness be 200-300 mesh.
7. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：It is described
Step（4）The temperature of middle refrigerating chamber is -30 DEG C of -65-, and cooling time is 24-48h, and the cryodesiccated time is 12-24h.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601977A (en) * | 2022-03-28 | 2022-06-10 | 西湖大学 | Whey protein biological implant material, preparation method thereof and biological implant prepared from material |
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